Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057665.t1 | AES89402.1 | 98.5 | 137 | 2 | 0 | 1 | 137 | 1 | 137 | 1.60E-67 | 265.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057665.t1 | Q84WS8 | 63.4 | 142 | 47 | 2 | 3 | 141 | 2 | 141 | 3.3e-42 | 172.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057665.t1 | A0A072URE6 | 98.5 | 137 | 2 | 0 | 1 | 137 | 254 | 390 | 1.1e-67 | 265.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04984 | MS.gene057665 | 0.816696 | 4.84E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene057665 | 0.801889 | 7.28E-49 | -1.69E-46 |
| MS.gene050762 | MS.gene057665 | 0.806033 | 1.00E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene057665 | 0.808592 | 2.87E-50 | -1.69E-46 |
| MS.gene052856 | MS.gene057665 | 0.813584 | 2.38E-51 | -1.69E-46 |
| MS.gene052858 | MS.gene057665 | 0.804773 | 1.84E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene057665 | 0.824427 | 8.12E-54 | -1.69E-46 |
| MS.gene057665 | MS.gene060084 | 0.808535 | 2.95E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene06739 | 0.800918 | 1.15E-48 | -1.69E-46 |
| MS.gene057665 | MS.gene06957 | 0.801354 | 9.37E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene072564 | 0.805951 | 1.04E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene072565 | 0.807248 | 5.54E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene07352 | -0.815649 | 8.29E-52 | -1.69E-46 |
| MS.gene057665 | MS.gene08608 | 0.813883 | 2.04E-51 | -1.69E-46 |
| MS.gene057665 | MS.gene09136 | 0.804004 | 2.66E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene21434 | -0.807449 | 5.03E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene27393 | 0.800397 | 1.47E-48 | -1.69E-46 |
| MS.gene057665 | MS.gene29986 | 0.800565 | 1.36E-48 | -1.69E-46 |
| MS.gene057665 | MS.gene31059 | 0.809602 | 1.74E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene32589 | 0.80284 | 4.63E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene35822 | 0.809782 | 1.60E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene35867 | 0.80487 | 1.75E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene42101 | 0.819152 | 1.35E-52 | -1.69E-46 |
| MS.gene057665 | MS.gene44007 | 0.835967 | 1.23E-56 | -1.69E-46 |
| MS.gene057665 | MS.gene45498 | 0.802735 | 4.87E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene48054 | 0.830039 | 3.68E-55 | -1.69E-46 |
| MS.gene057665 | MS.gene50595 | 0.804478 | 2.12E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene63887 | 0.838605 | 2.60E-57 | -1.69E-46 |
| MS.gene057665 | MS.gene68874 | 0.802641 | 5.10E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene68877 | 0.818822 | 1.60E-52 | -1.69E-46 |
| MS.gene057665 | MS.gene68933 | 0.817388 | 3.38E-52 | -1.69E-46 |
| MS.gene057665 | MS.gene68959 | 0.816828 | 4.52E-52 | -1.69E-46 |
| MS.gene057665 | MS.gene69935 | 0.811082 | 8.36E-51 | -1.69E-46 |
| MS.gene057665 | MS.gene70811 | 0.810592 | 1.07E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene70819 | 0.847772 | 9.36E-60 | -1.69E-46 |
| MS.gene057665 | MS.gene73273 | 0.830673 | 2.57E-55 | -1.69E-46 |
| MS.gene057665 | MS.gene75509 | 0.801897 | 7.25E-49 | -1.69E-46 |
| MS.gene057665 | MS.gene78247 | 0.809396 | 1.93E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene80298 | 0.822755 | 2.00E-53 | -1.69E-46 |
| MS.gene057665 | MS.gene81381 | 0.845777 | 3.29E-59 | -1.69E-46 |
| MS.gene057665 | MS.gene87037 | 0.809923 | 1.49E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene87771 | 0.810204 | 1.29E-50 | -1.69E-46 |
| MS.gene057665 | MS.gene99462 | 0.80762 | 4.62E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057665.t1 | MTR_4g074450 | 96.429 | 140 | 5 | 0 | 1 | 140 | 1 | 140 | 1.81e-97 | 277 |
| MS.gene057665.t1 | MTR_4g108000 | 96.429 | 140 | 5 | 0 | 1 | 140 | 254 | 393 | 5.86e-95 | 280 |
| MS.gene057665.t1 | MTR_4g108000 | 96.429 | 140 | 5 | 0 | 1 | 140 | 269 | 408 | 1.17e-94 | 279 |
| MS.gene057665.t1 | MTR_4g074450 | 92.254 | 142 | 9 | 1 | 1 | 142 | 1 | 140 | 9.07e-94 | 268 |
| MS.gene057665.t1 | MTR_4g108000 | 91.724 | 145 | 8 | 2 | 1 | 141 | 269 | 413 | 1.56e-90 | 270 |
| MS.gene057665.t1 | MTR_1g012600 | 44.048 | 84 | 45 | 1 | 49 | 132 | 47 | 128 | 1.28e-17 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057665.t1 | AT3G07860 | 63.380 | 142 | 47 | 2 | 3 | 141 | 2 | 141 | 1.52e-51 | 161 |
| MS.gene057665.t1 | AT3G07860 | 63.380 | 142 | 47 | 2 | 3 | 141 | 2 | 141 | 1.52e-51 | 161 |
| MS.gene057665.t1 | AT3G07860 | 65.414 | 133 | 43 | 1 | 3 | 132 | 2 | 134 | 3.49e-50 | 157 |
| MS.gene057665.t1 | AT5G25340 | 40.964 | 83 | 47 | 1 | 50 | 132 | 19 | 99 | 4.57e-17 | 74.7 |
| MS.gene057665.t1 | AT4G32270 | 33.333 | 84 | 54 | 1 | 49 | 132 | 37 | 118 | 5.69e-15 | 69.7 |
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGATGTGCTTCAAAATT+TGG | 0.144653 | 4.4:+34956824 | MS.gene057665:CDS |
| GTAATTAACTTTCTTCTTGA+TGG | 0.243951 | 4.4:-34956592 | None:intergenic |
| ATCTTCAAGCGTAGGATTCT+TGG | 0.255078 | 4.4:-34956448 | None:intergenic |
| AACTCTCATTGGACTTGAAT+TGG | 0.259182 | 4.4:+34956476 | MS.gene057665:CDS |
| TTGATTGCAGGAGGAGTGTT+TGG | 0.266654 | 4.4:+34956757 | MS.gene057665:intron |
| TTACATGGAACAATCTAGTA+TGG | 0.294231 | 4.4:+34956611 | MS.gene057665:CDS |
| GAAGGTGTTGAAAGCATTAT+TGG | 0.319812 | 4.4:+34956401 | MS.gene057665:CDS |
| TGGGGCATCGTCACATTTCT+TGG | 0.329427 | 4.4:+34956631 | MS.gene057665:CDS |
| TATCTATTCTATGTCATTGC+TGG | 0.345615 | 4.4:+34956879 | MS.gene057665:CDS |
| TATGAATACGGCAACGCTTA+AGG | 0.362851 | 4.4:+34956557 | MS.gene057665:CDS |
| TGATGTGATTCTTATGAATA+CGG | 0.363774 | 4.4:+34956545 | MS.gene057665:CDS |
| GAAGCACATCATCATCGTTA+AGG | 0.405288 | 4.4:-34956815 | None:intergenic |
| ACTCTCATTGGACTTGAATT+GGG | 0.418691 | 4.4:+34956477 | MS.gene057665:CDS |
| TTGGGTACATCTGCAAGTAT+TGG | 0.422385 | 4.4:-34956429 | None:intergenic |
| GATGATGTGCTTCAAAATTT+GGG | 0.438672 | 4.4:+34956825 | MS.gene057665:CDS |
| TGATTGCAGGAGGAGTGTTT+GGG | 0.443491 | 4.4:+34956758 | MS.gene057665:intron |
| TCTTCAAGCGTAGGATTCTT+GGG | 0.457805 | 4.4:-34956447 | None:intergenic |
| GGGCGTTCGAAATAATTCTC+AGG | 0.483534 | 4.4:+34956845 | MS.gene057665:CDS |
| ATACAAGAGCGGTTTGAAGA+AGG | 0.490103 | 4.4:+34956377 | MS.gene057665:CDS |
| GAAGATGTGGAAACTCTCAT+TGG | 0.517568 | 4.4:+34956465 | MS.gene057665:CDS |
| GAATCCTACGCTTGAAGATG+TGG | 0.526900 | 4.4:+34956452 | MS.gene057665:CDS |
| CAAGAAGAAAGTTAATTACA+TGG | 0.538196 | 4.4:+34956596 | MS.gene057665:CDS |
| GTTTCCACATCTTCAAGCGT+AGG | 0.543211 | 4.4:-34956456 | None:intergenic |
| TACATGGAACAATCTAGTAT+GGG | 0.546371 | 4.4:+34956612 | MS.gene057665:CDS |
| GGTGTTGAAAGCATTATTGG+AGG | 0.583098 | 4.4:+34956404 | MS.gene057665:CDS |
| TTGTGCGATGAGATGGGAGA+AGG | 0.646862 | 4.4:+34956348 | None:intergenic |
| ACATGGAACAATCTAGTATG+GGG | 0.649258 | 4.4:+34956613 | MS.gene057665:CDS |
| GAGCGGTTTGAAGAAGGCGA+AGG | 0.652966 | 4.4:+34956383 | MS.gene057665:CDS |
| CGATGAGATGGGAGAAGGTG+CGG | 0.664098 | 4.4:+34956353 | None:intergenic |
| GAAGGTGCGGAATACAAGAG+CGG | 0.727904 | 4.4:+34956366 | MS.gene057665:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAGAAGAAAGTTAATTACA+TGG | + | chr4.4:34956596-34956615 | MS.gene057665:CDS | 25.0% |
| ! | GTAATTAACTTTCTTCTTGA+TGG | - | chr4.4:34956595-34956614 | None:intergenic | 25.0% |
| ! | TGATGTGATTCTTATGAATA+CGG | + | chr4.4:34956545-34956564 | MS.gene057665:CDS | 25.0% |
| GATGATGTGCTTCAAAATTT+GGG | + | chr4.4:34956825-34956844 | MS.gene057665:CDS | 30.0% | |
| GCAACACATTGAAAAATGAT+TGG | + | chr4.4:34956699-34956718 | MS.gene057665:intron | 30.0% | |
| TACATGGAACAATCTAGTAT+GGG | + | chr4.4:34956612-34956631 | MS.gene057665:CDS | 30.0% | |
| TATCTATTCTATGTCATTGC+TGG | + | chr4.4:34956879-34956898 | MS.gene057665:CDS | 30.0% | |
| TTACATGGAACAATCTAGTA+TGG | + | chr4.4:34956611-34956630 | MS.gene057665:CDS | 30.0% | |
| ! | TGATGATGTGCTTCAAAATT+TGG | + | chr4.4:34956824-34956843 | MS.gene057665:CDS | 30.0% |
| ! | TTCAATGTGTTGCTAATCAA+TGG | - | chr4.4:34956692-34956711 | None:intergenic | 30.0% |
| !!! | ATCTGATTGATTTTGATTGC+AGG | + | chr4.4:34956745-34956764 | MS.gene057665:intron | 30.0% |
| !!! | GCGAATTTCTGTTTTGAAAT+TGG | + | chr4.4:34956509-34956528 | MS.gene057665:CDS | 30.0% |
| AACTCTCATTGGACTTGAAT+TGG | + | chr4.4:34956476-34956495 | MS.gene057665:CDS | 35.0% | |
| ACATGGAACAATCTAGTATG+GGG | + | chr4.4:34956613-34956632 | MS.gene057665:CDS | 35.0% | |
| ACTCTCATTGGACTTGAATT+GGG | + | chr4.4:34956477-34956496 | MS.gene057665:CDS | 35.0% | |
| !! | GAAGGTGTTGAAAGCATTAT+TGG | + | chr4.4:34956401-34956420 | MS.gene057665:CDS | 35.0% |
| !!! | TGATTGATTTTGATTGCAGG+AGG | + | chr4.4:34956748-34956767 | MS.gene057665:intron | 35.0% |
| ATACAAGAGCGGTTTGAAGA+AGG | + | chr4.4:34956377-34956396 | MS.gene057665:CDS | 40.0% | |
| ATCTTCAAGCGTAGGATTCT+TGG | - | chr4.4:34956451-34956470 | None:intergenic | 40.0% | |
| GAAGATGTGGAAACTCTCAT+TGG | + | chr4.4:34956465-34956484 | MS.gene057665:CDS | 40.0% | |
| GAAGCACATCATCATCGTTA+AGG | - | chr4.4:34956818-34956837 | None:intergenic | 40.0% | |
| TATGAATACGGCAACGCTTA+AGG | + | chr4.4:34956557-34956576 | MS.gene057665:CDS | 40.0% | |
| TCTTCAAGCGTAGGATTCTT+GGG | - | chr4.4:34956450-34956469 | None:intergenic | 40.0% | |
| TTGGGTACATCTGCAAGTAT+TGG | - | chr4.4:34956432-34956451 | None:intergenic | 40.0% | |
| !! | GGTGTTGAAAGCATTATTGG+AGG | + | chr4.4:34956404-34956423 | MS.gene057665:CDS | 40.0% |
| GAATCCTACGCTTGAAGATG+TGG | + | chr4.4:34956452-34956471 | MS.gene057665:CDS | 45.0% | |
| GGGCGTTCGAAATAATTCTC+AGG | + | chr4.4:34956845-34956864 | MS.gene057665:CDS | 45.0% | |
| GTTTCCACATCTTCAAGCGT+AGG | - | chr4.4:34956459-34956478 | None:intergenic | 45.0% | |
| !! | TGATTGCAGGAGGAGTGTTT+GGG | + | chr4.4:34956758-34956777 | MS.gene057665:intron | 45.0% |
| !! | TTGATTGCAGGAGGAGTGTT+TGG | + | chr4.4:34956757-34956776 | MS.gene057665:intron | 45.0% |
| ! | GAAGGTGCGGAATACAAGAG+CGG | + | chr4.4:34956366-34956385 | MS.gene057665:CDS | 50.0% |
| !! | TGGGGCATCGTCACATTTCT+TGG | + | chr4.4:34956631-34956650 | MS.gene057665:CDS | 50.0% |
| GAGCGGTTTGAAGAAGGCGA+AGG | + | chr4.4:34956383-34956402 | MS.gene057665:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 34956360 | 34956914 | 34956360 | ID=MS.gene057665 |
| chr4.4 | mRNA | 34956360 | 34956914 | 34956360 | ID=MS.gene057665.t1;Parent=MS.gene057665 |
| chr4.4 | exon | 34956360 | 34956652 | 34956360 | ID=MS.gene057665.t1.exon1;Parent=MS.gene057665.t1 |
| chr4.4 | CDS | 34956360 | 34956652 | 34956360 | ID=cds.MS.gene057665.t1;Parent=MS.gene057665.t1 |
| chr4.4 | exon | 34956767 | 34956914 | 34956767 | ID=MS.gene057665.t1.exon2;Parent=MS.gene057665.t1 |
| chr4.4 | CDS | 34956767 | 34956914 | 34956767 | ID=cds.MS.gene057665.t1;Parent=MS.gene057665.t1 |
| Gene Sequence |
| Protein sequence |
