AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22581.t1 MTR_5g011280 91.004 667 56 2 1 664 93 758 0.0 1213
MS.gene22581.t1 MTR_5g011270 80.995 663 124 2 1 663 93 753 0.0 1071
MS.gene22581.t1 MTR_5g011260 80.514 662 127 2 3 664 95 754 0.0 1068
MS.gene22581.t1 MTR_5g011340 66.018 668 219 4 2 663 87 752 0.0 894
MS.gene22581.t1 MTR_5g011320 66.467 668 215 5 2 663 83 747 0.0 893
MS.gene22581.t1 MTR_5g011190 66.218 669 218 4 2 664 90 756 0.0 891
MS.gene22581.t1 MTR_5g011310 65.269 668 224 4 2 663 90 755 0.0 891
MS.gene22581.t1 MTR_5g011370 66.317 668 217 4 2 663 110 775 0.0 889
MS.gene22581.t1 MTR_4g102400 60.118 677 246 13 1 663 88 754 0.0 779
MS.gene22581.t1 MTR_7g098630 44.684 696 343 15 1 662 74 761 0.0 565
MS.gene22581.t1 MTR_7g075900 44.733 693 346 13 1 663 88 773 0.0 540
MS.gene22581.t1 MTR_2g042130 43.453 695 352 13 3 663 87 774 2.29e-180 532
MS.gene22581.t1 MTR_5g016120 44.428 691 343 13 1 659 77 758 3.07e-177 523
MS.gene22581.t1 MTR_4g095360 43.290 693 351 15 1 661 72 754 1.32e-175 519
MS.gene22581.t1 MTR_8g021130 43.812 703 340 18 1 660 83 773 1.27e-172 512
MS.gene22581.t1 MTR_4g073540 41.926 706 356 17 1 661 71 767 3.82e-170 505
MS.gene22581.t1 MTR_4g125180 44.429 700 338 17 2 663 67 753 4.80e-169 502
MS.gene22581.t1 MTR_8g022870 44.272 707 344 16 1 661 73 775 5.30e-169 503
MS.gene22581.t1 MTR_0047s0120 43.353 692 351 14 1 660 73 755 1.34e-168 501
MS.gene22581.t1 MTR_1g102350 41.311 702 363 13 1 664 89 779 5.06e-164 490
MS.gene22581.t1 MTR_1g018510 42.143 700 355 13 2 660 34 724 9.42e-164 488
MS.gene22581.t1 MTR_3g102810 41.954 696 358 13 2 660 67 753 6.00e-157 471
MS.gene22581.t1 MTR_3g082200 39.568 695 378 13 1 661 83 769 5.93e-150 453
MS.gene22581.t1 MTR_8g072440 40.203 689 367 16 1 661 39 710 2.67e-147 444
MS.gene22581.t1 MTR_8g024420 39.747 712 343 21 1 661 45 721 4.55e-147 444
MS.gene22581.t1 MTR_8g072440 40.203 689 367 16 1 661 81 752 6.93e-147 445
MS.gene22581.t1 MTR_8g024390 38.659 701 368 18 1 661 79 757 4.02e-146 443
MS.gene22581.t1 MTR_8g083220 39.628 699 371 16 1 661 89 774 2.04e-145 442
MS.gene22581.t1 MTR_7g098070 37.393 698 386 15 1 661 78 761 1.67e-142 434
MS.gene22581.t1 MTR_1g079120 38.627 699 370 18 1 661 83 760 6.25e-142 432
MS.gene22581.t1 MTR_4g134320 37.911 699 382 15 1 661 81 765 1.04e-141 432
MS.gene22581.t1 MTR_7g118080 38.088 701 364 16 1 661 85 755 1.11e-141 432
MS.gene22581.t1 MTR_5g025010 40.091 656 350 17 1 626 44 686 4.71e-141 429
MS.gene22581.t1 MTR_8g079900 39.913 689 358 17 1 661 81 741 4.72e-141 429
MS.gene22581.t1 MTR_3g082200 39.602 654 353 13 42 661 1 646 9.72e-141 426
MS.gene22581.t1 MTR_5g025010 40.091 656 350 17 1 626 76 718 1.43e-140 429
MS.gene22581.t1 MTR_8g024380 38.000 700 368 15 1 661 86 758 1.44e-139 426
MS.gene22581.t1 MTR_2g049200 38.928 709 353 20 1 661 79 755 2.98e-139 425
MS.gene22581.t1 MTR_8g033150 39.623 689 360 16 1 661 64 724 3.75e-139 424
MS.gene22581.t1 MTR_8g033150 39.623 689 360 16 1 661 91 751 6.46e-139 424
MS.gene22581.t1 MTR_7g075890 38.752 689 366 16 1 661 237 897 2.32e-138 427
MS.gene22581.t1 MTR_7g104200 39.829 703 343 27 1 662 79 742 3.06e-138 422
MS.gene22581.t1 MTR_4g103490 39.466 712 372 17 1 663 84 785 2.60e-137 421
MS.gene22581.t1 MTR_1g078110 38.298 705 355 20 1 661 85 753 4.70e-137 419
MS.gene22581.t1 MTR_4g103450 39.230 701 379 15 1 663 54 745 7.25e-137 419
MS.gene22581.t1 MTR_3g104930 39.398 698 374 19 1 659 72 759 1.20e-136 419
MS.gene22581.t1 MTR_5g096750 40.086 696 367 18 1 661 84 764 1.40e-136 419
MS.gene22581.t1 MTR_8g079900 39.910 669 346 17 21 661 3 643 3.30e-136 414
MS.gene22581.t1 MTR_8g042670 38.808 688 383 15 1 661 81 757 2.83e-135 415
MS.gene22581.t1 MTR_4g103480 38.802 701 382 15 1 663 97 788 2.06e-134 414
MS.gene22581.t1 MTR_4g103495 42.373 649 328 14 1 610 81 722 3.03e-133 410
MS.gene22581.t1 MTR_4g103430 38.913 699 379 16 1 661 80 768 4.20e-133 410
MS.gene22581.t1 MTR_7g079310 37.074 704 374 20 1 661 86 763 5.66e-133 409
MS.gene22581.t1 MTR_3g082200 39.837 615 334 12 75 661 25 631 1.76e-132 404
MS.gene22581.t1 MTR_2g018125 40.195 719 364 19 1 663 77 785 6.70e-131 404
MS.gene22581.t1 MTR_4g103400 37.394 706 388 15 1 661 80 776 8.14e-131 404
MS.gene22581.t1 MTR_8g024370 39.045 712 353 25 1 661 75 756 1.75e-130 402
MS.gene22581.t1 MTR_2g018135 39.806 721 365 19 1 663 75 784 4.70e-130 402
MS.gene22581.t1 MTR_7g079300 37.677 706 366 21 1 661 89 765 8.03e-130 401
MS.gene22581.t1 MTR_8g042710 37.127 703 389 14 1 661 72 763 3.72e-128 397
MS.gene22581.t1 MTR_4g053630 39.093 706 360 24 1 661 49 729 6.06e-128 395
MS.gene22581.t1 MTR_3g114420 38.374 701 378 18 1 661 78 764 2.04e-127 395
MS.gene22581.t1 MTR_3g061110 37.698 695 377 18 1 661 80 752 1.63e-125 389
MS.gene22581.t1 MTR_4g103495 41.224 621 324 12 75 661 10 623 6.41e-125 384
MS.gene22581.t1 MTR_8g042670 38.684 623 349 13 62 661 21 633 7.25e-122 376
MS.gene22581.t1 MTR_4g053630 39.286 672 342 22 33 661 4 652 1.97e-121 376
MS.gene22581.t1 MTR_7g081420 37.691 719 372 23 1 661 84 784 2.80e-121 379
MS.gene22581.t1 MTR_7g081750 36.791 723 387 20 1 661 83 797 1.59e-120 378
MS.gene22581.t1 MTR_8g006680 37.500 672 381 18 1 661 79 722 6.35e-120 374
MS.gene22581.t1 MTR_8g005300 37.500 672 381 18 1 661 79 722 6.35e-120 374
MS.gene22581.t1 MTR_8g005640 37.500 672 381 18 1 661 79 722 6.35e-120 374
MS.gene22581.t1 MTR_4g053635 37.303 697 368 20 8 661 2 672 3.94e-119 370
MS.gene22581.t1 MTR_7g034380 36.095 676 392 15 1 661 41 691 5.47e-119 371
MS.gene22581.t1 MTR_5g055920 37.594 665 362 16 36 663 20 668 2.52e-118 368
MS.gene22581.t1 MTR_6g055570 35.942 690 372 18 1 661 81 729 5.59e-116 364
MS.gene22581.t1 MTR_1g007030 36.750 683 379 21 1 661 81 732 8.29e-116 363
MS.gene22581.t1 MTR_7g081500 37.604 718 373 25 1 661 83 782 1.21e-114 362
MS.gene22581.t1 MTR_1g048110 36.136 678 388 18 1 661 81 730 1.52e-114 360
MS.gene22581.t1 MTR_1g006990 36.589 686 378 22 1 661 81 734 2.80e-114 360
MS.gene22581.t1 MTR_5g085690 36.111 720 391 19 1 662 81 789 4.81e-114 360
MS.gene22581.t1 MTR_7g081580 36.886 713 385 24 1 661 84 783 1.35e-113 360
MS.gene22581.t1 MTR_7g081380 36.205 743 372 24 1 661 84 806 1.05e-112 357
MS.gene22581.t1 MTR_3g010080 36.765 680 373 19 1 656 80 726 2.09e-112 355
MS.gene22581.t1 MTR_8g005710 34.971 692 385 17 1 661 41 698 2.51e-111 351
MS.gene22581.t1 MTR_3g464790 38.150 692 356 22 1 659 77 729 1.85e-110 350
MS.gene22581.t1 MTR_7g481600 37.027 713 384 24 1 661 84 783 9.75e-109 347
MS.gene22581.t1 MTR_3g114410 43.160 519 241 17 1 481 78 580 5.58e-105 331
MS.gene22581.t1 MTR_6g055410 34.688 689 364 19 1 661 81 711 1.67e-103 331
MS.gene22581.t1 MTR_8g042710 39.771 523 269 12 1 484 72 587 2.14e-95 308
MS.gene22581.t1 MTR_2g044630 33.187 684 375 19 1 661 81 705 1.07e-90 297
MS.gene22581.t1 MTR_2g042640 35.906 635 308 23 1 615 50 605 4.99e-90 292
MS.gene22581.t1 MTR_2g046740 32.349 745 401 24 1 663 117 840 1.17e-85 286
MS.gene22581.t1 MTR_2g037850 34.100 739 383 31 1 659 97 811 2.34e-85 285
MS.gene22581.t1 MTR_3g452290 33.538 650 337 21 22 661 4 568 2.67e-84 276
MS.gene22581.t1 MTR_3g451220 33.538 650 337 21 22 661 4 568 2.67e-84 276
MS.gene22581.t1 MTR_4g071560 38.386 508 256 11 1 482 44 520 4.72e-84 275
MS.gene22581.t1 MTR_4g127480 33.605 735 392 32 1 659 97 811 2.64e-83 280
MS.gene22581.t1 MTR_8g089230 31.701 735 416 25 1 662 125 846 2.29e-81 275
MS.gene22581.t1 MTR_4g132620 30.323 742 412 25 1 662 99 815 1.27e-73 253
MS.gene22581.t1 MTR_2g042650 30.997 642 331 18 36 661 97 642 1.15e-69 239
MS.gene22581.t1 MTR_5g081100 36.364 429 213 12 1 417 46 426 1.29e-61 212
MS.gene22581.t1 MTR_7g079295 42.910 268 133 5 1 250 88 353 4.39e-55 196
MS.gene22581.t1 MTR_7g079295 35.294 153 87 4 515 661 363 509 1.50e-17 86.7
MS.gene22581.t1 MTR_8g027260 39.446 289 171 3 1 287 81 367 4.74e-55 199
MS.gene22581.t1 MTR_8g027260 38.944 303 161 9 371 661 361 651 5.78e-50 185
MS.gene22581.t1 MTR_6g075370 36.228 334 189 7 1 316 79 406 3.88e-53 194
MS.gene22581.t1 MTR_6g075370 39.841 251 132 3 394 633 421 663 1.46e-49 184
MS.gene22581.t1 MTR_5g011300 84.536 97 15 0 450 546 1 97 2.09e-49 167
MS.gene22581.t1 MTR_3g114160 40.333 300 141 7 1 294 15 282 6.70e-49 176
MS.gene22581.t1 MTR_2g070250 34.311 341 188 11 77 413 98 406 7.48e-39 148
MS.gene22581.t1 MTR_5g081280 35.741 263 127 9 1 252 66 297 4.54e-36 138
MS.gene22581.t1 MTR_4g126230 33.613 238 138 4 361 587 20 248 7.69e-36 135
MS.gene22581.t1 MTR_3g114140 35.938 256 148 6 375 617 169 421 3.98e-33 132
MS.gene22581.t1 MTR_3g114140 40.110 182 64 4 118 283 31 183 3.98e-22 100
MS.gene22581.t1 MTR_5g044060 47.794 136 62 2 387 513 3 138 9.67e-30 116
MS.gene22581.t1 MTR_8g092940 36.634 202 118 6 1 198 36 231 1.75e-28 114
MS.gene22581.t1 MTR_4g053665 33.597 253 118 9 416 661 1 210 4.51e-28 112
MS.gene22581.t1 MTR_2g070260 34.146 205 122 5 450 651 1 195 2.76e-26 107
MS.gene22581.t1 MTR_7g097250 32.864 213 103 7 258 467 1 176 5.57e-20 88.6
MS.gene22581.t1 MTR_5g081130 32.727 165 101 3 503 663 15 173 1.94e-19 87.4
MS.gene22581.t1 MTR_7g081660 36.025 161 95 6 503 661 398 552 8.12e-15 78.2
MS.gene22581.t1 MTR_3g065240 24.372 398 148 14 157 548 4 254 8.21e-14 72.8
MS.gene22581.t1 MTR_3g005755 64.444 45 16 0 442 486 238 282 2.56e-12 68.6
MS.gene22581.t1 MTR_5g011380 63.636 44 16 0 620 663 15 58 2.75e-12 63.2
MS.gene22581.t1 MTR_8g027660 27.179 195 107 7 474 661 80 246 2.40e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22581.t1 AT1G04110 43.652 701 346 17 1 661 79 770 0.0 546
MS.gene22581.t1 AT3G14067 44.349 699 343 15 1 661 77 767 0.0 538
MS.gene22581.t1 AT5G67360 44.348 690 347 14 1 661 73 754 0.0 536
MS.gene22581.t1 AT3G14240 43.059 706 355 16 1 663 70 771 1.69e-175 519
MS.gene22581.t1 AT2G05920 43.106 689 355 13 1 660 68 748 3.12e-173 513
MS.gene22581.t1 AT4G34980 41.261 698 368 13 1 661 66 758 5.62e-169 502
MS.gene22581.t1 AT5G51750 40.773 699 371 14 1 664 87 777 1.08e-158 476
MS.gene22581.t1 AT1G01900 41.176 697 366 16 1 663 87 773 1.66e-158 476
MS.gene22581.t1 AT5G59810 39.250 693 378 13 1 661 93 774 6.90e-148 448
MS.gene22581.t1 AT2G04160 40.202 694 376 14 1 661 83 770 4.46e-145 441
MS.gene22581.t1 AT5G59090 40.620 677 361 16 1 661 77 728 4.90e-140 427
MS.gene22581.t1 AT5G59090 40.325 677 361 17 1 661 77 726 6.34e-138 421
MS.gene22581.t1 AT5G59090 40.473 677 357 17 1 661 77 723 4.23e-137 419
MS.gene22581.t1 AT5G59120 39.678 683 359 17 1 661 76 727 7.19e-137 418
MS.gene22581.t1 AT5G03620 39.347 704 355 25 1 659 80 756 1.28e-135 416
MS.gene22581.t1 AT5G59100 39.124 685 367 17 1 661 79 737 1.74e-135 415
MS.gene22581.t1 AT1G20160 38.571 700 378 17 1 661 36 722 3.94e-135 414
MS.gene22581.t1 AT4G00230 39.913 689 359 19 1 661 82 743 5.44e-135 414
MS.gene22581.t1 AT1G20160 38.571 700 378 17 1 661 75 761 1.35e-134 414
MS.gene22581.t1 AT4G10550 38.298 705 373 19 1 661 24 710 3.57e-132 406
MS.gene22581.t1 AT4G10550 38.298 705 373 19 1 661 24 710 3.57e-132 406
MS.gene22581.t1 AT4G10550 38.298 705 373 19 1 661 80 766 1.18e-131 406
MS.gene22581.t1 AT4G10550 38.298 705 373 19 1 661 96 782 2.86e-131 405
MS.gene22581.t1 AT4G10520 37.736 689 382 15 1 661 75 744 1.63e-130 402
MS.gene22581.t1 AT5G67090 36.297 686 361 17 1 649 68 714 5.28e-129 398
MS.gene22581.t1 AT1G32960 37.660 701 384 15 1 661 78 765 6.04e-129 399
MS.gene22581.t1 AT4G10540 37.892 702 381 16 1 661 76 763 7.32e-129 399
MS.gene22581.t1 AT4G10510 38.177 702 377 17 1 661 9 694 9.01e-129 397
MS.gene22581.t1 AT4G10510 38.177 702 377 17 1 661 24 709 1.94e-128 396
MS.gene22581.t1 AT4G10510 38.177 702 377 17 1 661 68 753 5.22e-128 396
MS.gene22581.t1 AT4G10510 38.177 702 377 17 1 661 80 765 8.23e-128 396
MS.gene22581.t1 AT5G45650 39.535 731 357 20 1 661 73 788 1.56e-127 396
MS.gene22581.t1 AT5G45650 39.535 731 357 20 1 661 73 788 1.56e-127 396
MS.gene22581.t1 AT1G32940 37.090 701 388 17 1 661 24 711 3.94e-127 393
MS.gene22581.t1 AT1G32940 37.090 701 388 17 1 661 24 711 3.94e-127 393
MS.gene22581.t1 AT1G32940 37.090 701 388 17 1 661 75 762 2.40e-126 392
MS.gene22581.t1 AT5G59190 39.051 653 347 18 1 632 40 662 2.55e-126 390
MS.gene22581.t1 AT5G58840 38.166 676 354 19 1 661 80 706 5.42e-126 389
MS.gene22581.t1 AT5G59130 37.962 677 373 18 1 661 41 686 1.00e-124 386
MS.gene22581.t1 AT5G59130 37.962 677 373 18 1 661 80 725 2.58e-124 386
MS.gene22581.t1 AT3G46850 38.270 682 375 20 1 661 78 734 4.89e-124 385
MS.gene22581.t1 AT5G58830 37.463 678 357 18 1 661 78 705 5.36e-124 384
MS.gene22581.t1 AT1G32950 38.000 700 382 16 1 661 24 710 6.66e-124 384
MS.gene22581.t1 AT1G32950 38.000 700 382 16 1 661 75 761 1.50e-123 385
MS.gene22581.t1 AT3G46840 38.653 683 370 21 1 661 79 734 1.53e-122 381
MS.gene22581.t1 AT5G59130 37.962 677 369 19 1 661 80 721 1.61e-122 381
MS.gene22581.t1 AT1G20150 37.219 712 382 22 1 661 73 770 6.51e-122 381
MS.gene22581.t1 AT5G59130 37.962 677 363 20 1 661 80 715 7.73e-122 379
MS.gene22581.t1 AT4G10530 35.890 691 383 14 1 661 75 735 7.97e-122 380
MS.gene22581.t1 AT1G66220 36.127 692 381 14 1 661 83 744 3.04e-121 378
MS.gene22581.t1 AT4G21630 36.364 704 370 14 1 661 85 753 1.87e-118 371
MS.gene22581.t1 AT4G26330 36.582 708 387 18 3 663 31 723 6.68e-118 369
MS.gene22581.t1 AT4G21650 36.530 709 367 14 1 661 87 760 3.93e-117 368
MS.gene22581.t1 AT5G58820 37.574 676 358 17 1 661 74 700 1.45e-116 365
MS.gene22581.t1 AT1G66210 37.000 700 367 19 1 661 86 750 3.81e-115 363
MS.gene22581.t1 AT4G21326 36.075 693 382 15 1 661 24 687 1.12e-114 359
MS.gene22581.t1 AT4G26330 35.873 722 387 19 3 663 31 737 6.02e-114 359
MS.gene22581.t1 AT5G03620 44.031 511 241 18 1 481 80 575 3.49e-113 352
MS.gene22581.t1 AT5G11940 35.530 698 385 17 1 661 83 752 1.42e-112 356
MS.gene22581.t1 AT4G21323 35.622 699 381 22 1 661 126 793 2.43e-107 343
MS.gene22581.t1 AT4G15040 35.788 679 384 18 1 661 41 685 9.80e-107 338
MS.gene22581.t1 AT4G15040 35.788 679 384 18 1 661 41 685 9.80e-107 338
MS.gene22581.t1 AT5G45640 35.782 735 383 22 1 664 38 754 1.60e-106 340
MS.gene22581.t1 AT1G32970 35.863 672 360 18 29 661 83 722 1.84e-105 337
MS.gene22581.t1 AT4G21640 34.517 704 355 14 1 661 87 727 1.22e-104 334
MS.gene22581.t1 AT4G00230 43.141 503 248 14 1 481 82 568 2.18e-102 324
MS.gene22581.t1 AT5G58840 39.431 492 253 11 1 484 80 534 1.80e-94 302
MS.gene22581.t1 AT1G32940 38.343 519 273 14 1 481 75 584 1.49e-89 291
MS.gene22581.t1 AT2G39850 31.623 721 405 24 1 661 73 765 2.32e-89 295
MS.gene22581.t1 AT2G19170 33.199 744 384 29 1 659 93 808 6.54e-87 289
MS.gene22581.t1 AT2G19170 33.199 744 384 29 1 659 93 808 6.54e-87 289
MS.gene22581.t1 AT1G30600 31.684 748 402 24 1 663 105 828 1.80e-83 280
MS.gene22581.t1 AT4G20430 31.436 738 414 26 1 661 126 848 7.04e-83 279
MS.gene22581.t1 AT4G20430 31.436 738 414 26 1 661 126 848 7.04e-83 279
MS.gene22581.t1 AT4G30020 33.850 709 370 29 1 629 93 782 1.22e-82 278
MS.gene22581.t1 AT4G30020 33.850 709 370 29 1 629 93 782 1.22e-82 278
MS.gene22581.t1 AT4G30020 33.850 709 370 29 1 629 93 782 1.22e-82 278
MS.gene22581.t1 AT4G30020 33.850 709 370 29 1 629 93 782 1.22e-82 278
MS.gene22581.t1 AT1G20160 40.498 442 230 10 1 417 75 508 1.93e-81 268
MS.gene22581.t1 AT5G44530 30.311 739 421 24 1 662 113 834 2.47e-81 275
MS.gene22581.t1 AT5G44530 29.450 764 420 26 1 662 113 859 1.35e-76 262
MS.gene22581.t1 AT1G62340 32.392 744 401 28 1 661 105 829 1.17e-70 246
MS.gene22581.t1 AT5G58840 37.560 418 223 10 1 417 80 460 5.23e-67 228
MS.gene22581.t1 AT5G44530 30.769 494 276 15 1 434 113 600 3.70e-48 179
MS.gene22581.t1 AT4G20430 37.050 278 145 6 1 250 126 401 7.48e-43 166
MS.gene22581.t1 AT4G20430 33.333 285 161 11 397 661 549 824 2.25e-33 137
MS.gene22581.t1 AT1G32980 43.548 186 100 3 446 628 3 186 4.68e-41 148
MS.gene22581.t1 AT5G59110 35.928 167 93 7 506 664 8 168 3.86e-23 97.4

Find 168 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAGAGCCTTTGCTTCTTC+TGG 0.112206 5.2:+4047380 None:intergenic
ACCTCTATTCTCTTTGTCTT+TGG 0.124601 5.2:+4045519 None:intergenic
GGATCTATCAAGTGGGTTTC+TGG 0.160470 5.2:-4045478 MS.gene22581:CDS
TCAGAATTCCTGGGCTTTCT+TGG 0.175760 5.2:+4046181 None:intergenic
GTACTTTCAATCTCCCTTAA+TGG 0.186326 5.2:-4046846 MS.gene22581:CDS
TTATTCAAAGGAACTGTTAT+TGG 0.212356 5.2:-4046252 MS.gene22581:CDS
AATCACCTGGTTTAAGTCTT+TGG 0.215322 5.2:+4045926 None:intergenic
CTCGGTAAAGGGATCATAAT+TGG 0.217745 5.2:-4047242 MS.gene22581:CDS
CCTCTATTCTCTTTGTCTTT+GGG 0.245793 5.2:+4045520 None:intergenic
CCGCCATATAACATTATTTC+AGG 0.247732 5.2:-4046090 MS.gene22581:CDS
AGTTGCTCAGCTGGAAATTT+TGG 0.249923 5.2:-4046750 MS.gene22581:CDS
CTTAACATCAACGTTCTTCA+TGG 0.254863 5.2:+4046503 None:intergenic
GGGCCTTCCAGCGGCCGTGC+TGG 0.266267 5.2:+4047028 None:intergenic
TATGATCCCTTTACCGAGAT+TGG 0.274813 5.2:+4047247 None:intergenic
CTAGAGCCTTTGCTTCTTCT+GGG 0.278482 5.2:+4047381 None:intergenic
TGATCCAGGTTCTGATGTTC+AGG 0.285228 5.2:-4046364 MS.gene22581:CDS
AAGGGGTGGAGGGTCCATTA+AGG 0.291400 5.2:+4046832 None:intergenic
TGAGTTTATATAATCTTTGA+TGG 0.297149 5.2:+4046299 None:intergenic
CTCTTTGTCTTTGGGTGTAA+TGG 0.300037 5.2:+4045528 None:intergenic
ACTCACACACCAAGCTTCTT+GGG 0.311370 5.2:-4047302 MS.gene22581:CDS
ATGTCTGGTTTCAGAATTCC+TGG 0.311991 5.2:+4046171 None:intergenic
AACTCACACACCAAGCTTCT+TGG 0.313947 5.2:-4047303 MS.gene22581:CDS
CATGAAGAACGTTGATGTTA+AGG 0.314329 5.2:-4046502 MS.gene22581:CDS
TCAACGTTCTTCATGGAAAT+AGG 0.324824 5.2:+4046510 None:intergenic
TAGATTGCCAAATGTGCTTC+TGG 0.329353 5.2:+4046951 None:intergenic
TGCAACAGAGTCGAGGATTA+TGG 0.343350 5.2:-4047277 MS.gene22581:CDS
ATTCCTGGGCTTTCTTGGCT+TGG 0.349261 5.2:+4046186 None:intergenic
ATTCTGAAACCAGACATAAT+TGG 0.361585 5.2:-4046165 MS.gene22581:CDS
CATGGCACCAGAAGCACATT+TGG 0.372753 5.2:-4046958 MS.gene22581:CDS
GTACCTGAAATAATGTTATA+TGG 0.374557 5.2:+4046087 None:intergenic
TGCATTTAGTGCAAATCAAA+AGG 0.376754 5.2:-4046784 MS.gene22581:CDS
TAATTTAAACCACACAAATA+AGG 0.380966 5.2:+4045817 None:intergenic
ATGAAGAACGTTGATGTTAA+GGG 0.383158 5.2:-4046501 MS.gene22581:CDS
CTCATGAATGGTAAACTTCA+AGG 0.387470 5.2:-4046390 MS.gene22581:CDS
AATTATGACAACTGCTTATG+AGG 0.393448 5.2:-4045977 MS.gene22581:CDS
TTAGACGTTGTAATATAATC+AGG 0.395652 5.2:+4046726 None:intergenic
TTCTTCTGGGGTCAATTTCA+CGG 0.399692 5.2:+4047394 None:intergenic
CCAGGTGATTTATTTGCAAC+TGG 0.402856 5.2:-4045913 MS.gene22581:CDS
TGTAGAAATTGATGTTCCTT+TGG 0.403989 5.2:-4045623 MS.gene22581:CDS
TGCCTTCTCAACCTGCGAAA+TGG 0.404789 5.2:-4047166 MS.gene22581:CDS
GCTTATGAGGTTAATCTTCA+AGG 0.405892 5.2:-4045964 MS.gene22581:CDS
GGATAATTTAGTTGCGCCTC+TGG 0.406130 5.2:+4045730 None:intergenic
ATTTGTAACAAGAAGATCAT+TGG 0.406451 5.2:-4047113 MS.gene22581:CDS
AAATAAGGAACATAATCATT+TGG 0.413071 5.2:+4045832 None:intergenic
GATTTATTTGCAACTGGTGC+TGG 0.417610 5.2:-4045907 MS.gene22581:CDS
ACTCCAGGTCCAATTATGTC+TGG 0.423015 5.2:+4046156 None:intergenic
AGTGCTAATGGTACTGCAAT+AGG 0.426292 5.2:-4046981 MS.gene22581:CDS
CAATAGCTAAACTTAGCAAT+GGG 0.426944 5.2:-4046647 MS.gene22581:CDS
GTGCAAGGTGCTAATGTCTA+TGG 0.428109 5.2:-4047005 MS.gene22581:CDS
AGGTGATAACAAAATTTCTC+AGG 0.428850 5.2:-4045500 MS.gene22581:CDS
TCATACCAAAACGTCATGAA+TGG 0.429593 5.2:-4047422 None:intergenic
GAACATAATCATTTGGTTCA+AGG 0.430056 5.2:+4045839 None:intergenic
TCAGTGAATGTTATTTGAGC+AGG 0.430511 5.2:+4045580 None:intergenic
CCTGGATCGTTTGCTTTCGA+AGG 0.435626 5.2:+4045874 None:intergenic
GCAATTACATCTTCAAAGAA+AGG 0.436124 5.2:+4046813 None:intergenic
TCCCTTCCATATGATGATAA+TGG 0.440303 5.2:-4047068 MS.gene22581:CDS
AATTATACTGATAGACAAGT+TGG 0.443269 5.2:-4045799 MS.gene22581:CDS
AAAATTCCAATCTCGGTAAA+GGG 0.444969 5.2:-4047253 MS.gene22581:CDS
AGTGTTGATGCAAAGTCTTT+TGG 0.446736 5.2:+4046594 None:intergenic
ATAGCAGCTGGAGACCAATC+TGG 0.448497 5.2:+4046009 None:intergenic
AGCAGCATGGCATGCATCAT+TGG 0.455904 5.2:-4046124 MS.gene22581:CDS
AATGTCTATGGCAGTGCTAA+TGG 0.456995 5.2:-4046993 MS.gene22581:CDS
AGGGATCATAATTGGAATCC+TGG 0.459598 5.2:-4047234 MS.gene22581:CDS
CCATGACCATTATCATCATA+TGG 0.465367 5.2:+4047062 None:intergenic
ATTGCTGATTTAATAGCAGC+TGG 0.469867 5.2:+4045997 None:intergenic
TTGAATAAAATTGTGGCCAT+TGG 0.470772 5.2:+4046267 None:intergenic
ACTAGAACATTGACGAATGT+TGG 0.473220 5.2:-4045667 MS.gene22581:CDS
CAATTACATCTTCAAAGAAA+GGG 0.481376 5.2:+4046814 None:intergenic
GATGAAAGTGCAGCATTAGC+AGG 0.483030 5.2:-4046900 MS.gene22581:CDS
AGGGGTGGAGGGTCCATTAA+GGG 0.483877 5.2:+4046833 None:intergenic
CTGGATCGTTTGCTTTCGAA+GGG 0.488084 5.2:+4045875 None:intergenic
CAGTGGCCGTTCCATTTCGC+AGG 0.488156 5.2:+4047155 None:intergenic
CACACAGCCAGCACGGCCGC+TGG 0.491935 5.2:-4047035 MS.gene22581:CDS
TTTACCATTCATGAGGATCA+TGG 0.499927 5.2:+4046398 None:intergenic
CAGAAAAGGAAGGATGGGAG+AGG 0.502238 5.2:+4047198 None:intergenic
CATGGAAATAGGACCACAAA+AGG 0.502542 5.2:+4046521 None:intergenic
AGGCATTCCCTCGTCAGAAA+AGG 0.503857 5.2:+4047184 None:intergenic
ATCTTCAAAGAAAGGGGTGG+AGG 0.509300 5.2:+4046821 None:intergenic
CCATATGATGATAATGGTCA+TGG 0.510595 5.2:-4047062 MS.gene22581:CDS
GCCGCTGGAAGGCCCGTGCA+AGG 0.511491 5.2:-4047020 MS.gene22581:CDS
TCAAGGTCATAAACAAGTCC+TGG 0.511948 5.2:+4045856 None:intergenic
ACTTTCATCACAACGACCAC+TGG 0.513443 5.2:+4046914 None:intergenic
AGTTCCTTTGAATAAAATTG+TGG 0.515236 5.2:+4046260 None:intergenic
GACCATTATCATCATATGGA+AGG 0.518281 5.2:+4047066 None:intergenic
TGTCTGGTTTCAGAATTCCT+GGG 0.519701 5.2:+4046172 None:intergenic
TTCCATTTCGCAGGTTGAGA+AGG 0.521348 5.2:+4047164 None:intergenic
CTCTGGTATGCTCTTTACCT+CGG 0.522478 5.2:+4045747 None:intergenic
CCTTCGAAAGCAAACGATCC+AGG 0.522836 5.2:-4045874 MS.gene22581:CDS
GAGTAACTTACATGTGTAGC+AGG 0.522925 5.2:+4046333 None:intergenic
ATGAAAAGTAAGCTACTTGA+GGG 0.525431 5.2:+4046217 None:intergenic
ACATTGTAAGTCATGTTGAC+AGG 0.526484 5.2:+4045643 None:intergenic
CAGCCAGCACGGCCGCTGGA+AGG 0.528367 5.2:-4047031 MS.gene22581:CDS
GATCCAGGTTCTGATGTTCA+GGG 0.529907 5.2:-4046363 MS.gene22581:CDS
CCCAAAGACAAAGAGAATAG+AGG 0.530769 5.2:-4045520 MS.gene22581:CDS
GCAATAGCTAAACTTAGCAA+TGG 0.531778 5.2:-4046648 MS.gene22581:CDS
TGTTGCAACCCCAAGAAGCT+TGG 0.535770 5.2:+4047293 None:intergenic
AGAGGGATATTCCTGTGTCC+AGG 0.538307 5.2:+4047216 None:intergenic
ATAGACATTAGCACCTTGCA+CGG 0.544705 5.2:+4047007 None:intergenic
AAGTGCAGCATTAGCAGGAA+TGG 0.545404 5.2:-4046895 MS.gene22581:CDS
CCTCGTCAGAAAAGGAAGGA+TGG 0.547531 5.2:+4047192 None:intergenic
ACACATGTAAGTTACTCTGC+TGG 0.549190 5.2:-4046327 MS.gene22581:CDS
GGACCAAGCCAAGAAAGCCC+AGG 0.552124 5.2:-4046189 MS.gene22581:CDS
CCAGTTGCAAATAAATCACC+TGG 0.552195 5.2:+4045913 None:intergenic
CTCGTCAGAAAAGGAAGGAT+GGG 0.555705 5.2:+4047193 None:intergenic
AGCAAAGGCTCTAGAAGAGA+AGG 0.561267 5.2:-4047372 MS.gene22581:CDS
GCATTTAGTGCAAATCAAAA+GGG 0.562724 5.2:-4046783 MS.gene22581:CDS
TGGAAGGGATGAATTTACTA+AGG 0.563042 5.2:+4047082 None:intergenic
TAGAGCCTTTGCTTCTTCTG+GGG 0.563775 5.2:+4047382 None:intergenic
ACCTTGCACGGGCCTTCCAG+CGG 0.564302 5.2:+4047019 None:intergenic
GAACGTTGATGTTAAGGGAA+AGG 0.564400 5.2:-4046496 MS.gene22581:CDS
GATTATGTTCCTTATTTGTG+TGG 0.564467 5.2:-4045826 MS.gene22581:CDS
GATGAAAAGTAAGCTACTTG+AGG 0.564885 5.2:+4046216 None:intergenic
AACGGCCACTGTGAATTCAC+AGG 0.565850 5.2:-4047143 MS.gene22581:CDS
GCTGCTAGAATGTTCACTCC+AGG 0.567644 5.2:+4046141 None:intergenic
CTCACACACCAAGCTTCTTG+GGG 0.569819 5.2:-4047301 MS.gene22581:CDS
TTTCTCAGGGATCTATCAAG+TGG 0.570728 5.2:-4045486 MS.gene22581:CDS
CTTGAAGTTTACCATTCATG+AGG 0.579948 5.2:+4046391 None:intergenic
GGTGATAACAAAATTTCTCA+GGG 0.581829 5.2:-4045499 MS.gene22581:CDS
ACCATTATCATCATATGGAA+GGG 0.582756 5.2:+4047067 None:intergenic
TCTTCAAAGAAAGGGGTGGA+GGG 0.584147 5.2:+4046822 None:intergenic
CTGTTGCCACTTGTGTATGC+AGG 0.587692 5.2:-4046573 MS.gene22581:CDS
AAACTCAACTTCAACACCAA+TGG 0.587710 5.2:-4046283 MS.gene22581:CDS
ACTGTGAATTCACAGGGGAA+AGG 0.589203 5.2:-4047136 MS.gene22581:CDS
TTTGAAGATGTAATTGCACT+TGG 0.595521 5.2:-4046807 MS.gene22581:CDS
AATAGGACCACAAAAGGCAA+TGG 0.596454 5.2:+4046527 None:intergenic
TTCTCAGGGATCTATCAAGT+GGG 0.598929 5.2:-4045485 MS.gene22581:CDS
TAAGCTACTTGAGGGGCGAG+TGG 0.600122 5.2:+4046225 None:intergenic
ACGCCCTGAACATCAGAACC+TGG 0.602996 5.2:+4046360 None:intergenic
TTGGGGTTGCAACAGAGTCG+AGG 0.603368 5.2:-4047284 MS.gene22581:CDS
AGAAAAGGAAGGATGGGAGA+GGG 0.610457 5.2:+4047199 None:intergenic
GGCTCTAGAAGAGAAGGAGG+AGG 0.610956 5.2:-4047366 MS.gene22581:CDS
GCACCAACAGTTAGAATCCA+CGG 0.611936 5.2:+4046696 None:intergenic
TGGAACTCACACAGCCAGCA+CGG 0.614452 5.2:-4047042 MS.gene22581:CDS
ACAGAACCTGCATACACAAG+TGG 0.615610 5.2:+4046567 None:intergenic
ATTGACCCCAGAAGAAGCAA+AGG 0.616646 5.2:-4047387 MS.gene22581:CDS
TACATCTTCAAAGAAAGGGG+TGG 0.616672 5.2:+4046818 None:intergenic
GACAGATATAGGTATTCTAA+CGG 0.618937 5.2:+4045447 None:intergenic
AAAGGCTCTAGAAGAGAAGG+AGG 0.622215 5.2:-4047369 MS.gene22581:CDS
TAGACATTAGCACCTTGCAC+GGG 0.627751 5.2:+4047008 None:intergenic
GAGTGAACATTCTAGCAGCA+TGG 0.629667 5.2:-4046137 MS.gene22581:CDS
CAAGCAGTTAAAGACGCTGG+TGG 0.630093 5.2:-4046426 MS.gene22581:CDS
GCTCAAGCAGTTAAAGACGC+TGG 0.632815 5.2:-4046429 MS.gene22581:CDS
ATGTCTTGTCCTCATCTCAG+TGG 0.632923 5.2:-4046060 MS.gene22581:CDS
ACGGCCACTGTGAATTCACA+GGG 0.634794 5.2:-4047142 MS.gene22581:CDS
ATAGTGATATCAACTGCCAA+AGG 0.636493 5.2:+4045607 None:intergenic
TAATGGTACTGCAATAGGCA+TGG 0.638046 5.2:-4046976 MS.gene22581:CDS
ATAATTGGAATCCTGGACAC+AGG 0.640358 5.2:-4047227 MS.gene22581:CDS
AAACCAGACATAATTGGACC+TGG 0.641671 5.2:-4046159 MS.gene22581:CDS
GCTGCCATGATCCTCATGAA+TGG 0.641925 5.2:-4046402 MS.gene22581:CDS
GCAGCAATTCCACTGAGATG+AGG 0.644059 5.2:+4046051 None:intergenic
TCTCAACCTGCGAAATGGAA+CGG 0.644123 5.2:-4047161 MS.gene22581:CDS
ACAAGAAGATCATTGGAGCA+AGG 0.644785 5.2:-4047106 MS.gene22581:CDS
ATGGACGCTGCAGTTGAAGA+CGG 0.649497 5.2:-4046876 MS.gene22581:CDS
ATTCCCTCGTCAGAAAAGGA+AGG 0.650060 5.2:+4047188 None:intergenic
GAGATGAGGACAAGACATTG+AGG 0.654510 5.2:+4046065 None:intergenic
TACAAAGTATGCGCAACCAG+TGG 0.656203 5.2:-4046930 MS.gene22581:CDS
TGTGAGTTGTATGTAATGAG+AGG 0.657109 5.2:+4047318 None:intergenic
AATTACATCTTCAAAGAAAG+GGG 0.660792 5.2:+4046815 None:intergenic
GCGCCGTGGATTCTAACTGT+TGG 0.673712 5.2:-4046699 MS.gene22581:CDS
CCTGAAATAATGTTATATGG+CGG 0.691602 5.2:+4046090 None:intergenic
CGAGGTAAAGAGCATACCAG+AGG 0.691664 5.2:-4045746 MS.gene22581:CDS
CAACGTCTAATGAAGCGCCG+TGG 0.694575 5.2:-4046713 MS.gene22581:CDS
AATAGCTAAACTTAGCAATG+GGG 0.694754 5.2:-4046646 MS.gene22581:CDS
TTAGACCAAAGACTTAAACC+AGG 0.738619 5.2:-4045931 MS.gene22581:CDS
TGAAAAGTAAGCTACTTGAG+GGG 0.748832 5.2:+4046218 None:intergenic
TTTCCCCTGTGAATTCACAG+TGG 0.753630 5.2:+4047138 None:intergenic
ACATAAAGTGAAATGCTCCG+AGG 0.760693 5.2:-4045764 MS.gene22581:CDS
CGGCCACTGTGAATTCACAG+GGG 0.810723 5.2:-4047141 MS.gene22581:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAGGAACATAATCATT+TGG + chr5.2:4047013-4047032 None:intergenic 20.0%
!! TAATTTAAACCACACAAATA+AGG + chr5.2:4047028-4047047 None:intergenic 20.0%
!! TGAGTTTATATAATCTTTGA+TGG + chr5.2:4046546-4046565 None:intergenic 20.0%
!!! CATCATTTTCTATTTTTTTG+GGG - chr5.2:4047134-4047153 MS.gene22581:CDS 20.0%
!!! CCATCATTTTCTATTTTTTT+GGG - chr5.2:4047133-4047152 MS.gene22581:CDS 20.0%
!!! TCCATCATTTTCTATTTTTT+TGG - chr5.2:4047132-4047151 MS.gene22581:CDS 20.0%
! AATTACATCTTCAAAGAAAG+GGG + chr5.2:4046030-4046049 None:intergenic 25.0%
! AATTATACTGATAGACAAGT+TGG - chr5.2:4047043-4047062 MS.gene22581:CDS 25.0%
! AGTTCCTTTGAATAAAATTG+TGG + chr5.2:4046585-4046604 None:intergenic 25.0%
! ATTTGTAACAAGAAGATCAT+TGG - chr5.2:4045729-4045748 MS.gene22581:CDS 25.0%
! CAATTACATCTTCAAAGAAA+GGG + chr5.2:4046031-4046050 None:intergenic 25.0%
! CCCAAAAAAATAGAAAATGA+TGG + chr5.2:4047136-4047155 None:intergenic 25.0%
! GTACCTGAAATAATGTTATA+TGG + chr5.2:4046758-4046777 None:intergenic 25.0%
! TTAGACGTTGTAATATAATC+AGG + chr5.2:4046119-4046138 None:intergenic 25.0%
! TTATGGAAAAATTCCAATCT+CGG - chr5.2:4045582-4045601 MS.gene22581:CDS 25.0%
! TTATTCAAAGGAACTGTTAT+TGG - chr5.2:4046590-4046609 MS.gene22581:CDS 25.0%
!!! TATTGCTTCTATTTTTCTGT+CGG + chr5.2:4046180-4046199 None:intergenic 25.0%
AAAAATTCCAATCTCGGTAA+AGG - chr5.2:4045588-4045607 MS.gene22581:CDS 30.0%
AAAATTCCAATCTCGGTAAA+GGG - chr5.2:4045589-4045608 MS.gene22581:CDS 30.0%
AATAGCTAAACTTAGCAATG+GGG - chr5.2:4046196-4046215 MS.gene22581:CDS 30.0%
AATTATGACAACTGCTTATG+AGG - chr5.2:4046865-4046884 MS.gene22581:CDS 30.0%
ACCATTATCATCATATGGAA+GGG + chr5.2:4045778-4045797 None:intergenic 30.0%
AGCAATGGGGAAAAATATAT+TGG - chr5.2:4046209-4046228 MS.gene22581:CDS 30.0%
AGGTGATAACAAAATTTCTC+AGG - chr5.2:4047342-4047361 MS.gene22581:CDS 30.0%
ATGAAAAGTAAGCTACTTGA+GGG + chr5.2:4046628-4046647 None:intergenic 30.0%
ATGAAGAACGTTGATGTTAA+GGG - chr5.2:4046341-4046360 MS.gene22581:CDS 30.0%
CAATAGCTAAACTTAGCAAT+GGG - chr5.2:4046195-4046214 MS.gene22581:CDS 30.0%
CACACAAAAATGACAGATAT+AGG + chr5.2:4047409-4047428 None:intergenic 30.0%
CCTGAAATAATGTTATATGG+CGG + chr5.2:4046755-4046774 None:intergenic 30.0%
GAAGCAAAAAGTTACATACT+CGG - chr5.2:4047288-4047307 MS.gene22581:CDS 30.0%
GACAGATATAGGTATTCTAA+CGG + chr5.2:4047398-4047417 None:intergenic 30.0%
GCAATTACATCTTCAAAGAA+AGG + chr5.2:4046032-4046051 None:intergenic 30.0%
GCATTTAGTGCAAATCAAAA+GGG - chr5.2:4046059-4046078 MS.gene22581:CDS 30.0%
GGTGATAACAAAATTTCTCA+GGG - chr5.2:4047343-4047362 MS.gene22581:CDS 30.0%
TGCATTTAGTGCAAATCAAA+AGG - chr5.2:4046058-4046077 MS.gene22581:CDS 30.0%
TGTAGAAATTGATGTTCCTT+TGG - chr5.2:4047219-4047238 MS.gene22581:CDS 30.0%
TTGAATAAAATTGTGGCCAT+TGG + chr5.2:4046578-4046597 None:intergenic 30.0%
TTTGAAGATGTAATTGCACT+TGG - chr5.2:4046035-4046054 MS.gene22581:CDS 30.0%
! ATGGCCACAATTTTATTCAA+AGG - chr5.2:4046578-4046597 MS.gene22581:CDS 30.0%
! GAACATAATCATTTGGTTCA+AGG + chr5.2:4047006-4047025 None:intergenic 30.0%
! GATTATGTTCCTTATTTGTG+TGG - chr5.2:4047016-4047035 MS.gene22581:CDS 30.0%
!! ATTCTGAAACCAGACATAAT+TGG - chr5.2:4046677-4046696 MS.gene22581:CDS 30.0%
AAACTCAACTTCAACACCAA+TGG - chr5.2:4046559-4046578 MS.gene22581:CDS 35.0%
AATCACCTGGTTTAAGTCTT+TGG + chr5.2:4046919-4046938 None:intergenic 35.0%
ACATTGTAAGTCATGTTGAC+AGG + chr5.2:4047202-4047221 None:intergenic 35.0%
ACCTCTATTCTCTTTGTCTT+TGG + chr5.2:4047326-4047345 None:intergenic 35.0%
ACTAGAACATTGACGAATGT+TGG - chr5.2:4047175-4047194 MS.gene22581:CDS 35.0%
ATAGTGATATCAACTGCCAA+AGG + chr5.2:4047238-4047257 None:intergenic 35.0%
CATGAAGAACGTTGATGTTA+AGG - chr5.2:4046340-4046359 MS.gene22581:CDS 35.0%
CCATGACCATTATCATCATA+TGG + chr5.2:4045783-4045802 None:intergenic 35.0%
CCGCCATATAACATTATTTC+AGG - chr5.2:4046752-4046771 MS.gene22581:CDS 35.0%
CCTCTATTCTCTTTGTCTTT+GGG + chr5.2:4047325-4047344 None:intergenic 35.0%
CTCATGAATGGTAAACTTCA+AGG - chr5.2:4046452-4046471 MS.gene22581:CDS 35.0%
CTTAACATCAACGTTCTTCA+TGG + chr5.2:4046342-4046361 None:intergenic 35.0%
CTTGAAGTTTACCATTCATG+AGG + chr5.2:4046454-4046473 None:intergenic 35.0%
GACCATTATCATCATATGGA+AGG + chr5.2:4045779-4045798 None:intergenic 35.0%
GATGAAAAGTAAGCTACTTG+AGG + chr5.2:4046629-4046648 None:intergenic 35.0%
GCAATAGCTAAACTTAGCAA+TGG - chr5.2:4046194-4046213 MS.gene22581:CDS 35.0%
GCTTATGAGGTTAATCTTCA+AGG - chr5.2:4046878-4046897 MS.gene22581:CDS 35.0%
GTACTTTCAATCTCCCTTAA+TGG - chr5.2:4045996-4046015 MS.gene22581:CDS 35.0%
TCAACGTTCTTCATGGAAAT+AGG + chr5.2:4046335-4046354 None:intergenic 35.0%
TCCCTTCCATATGATGATAA+TGG - chr5.2:4045774-4045793 MS.gene22581:CDS 35.0%
TGAAAAACTCTCATCCAGAT+TGG - chr5.2:4046819-4046838 MS.gene22581:CDS 35.0%
TGAAAAGTAAGCTACTTGAG+GGG + chr5.2:4046627-4046646 None:intergenic 35.0%
TGGAAGGGATGAATTTACTA+AGG + chr5.2:4045763-4045782 None:intergenic 35.0%
TGTGAGTTGTATGTAATGAG+AGG + chr5.2:4045527-4045546 None:intergenic 35.0%
TTAGACCAAAGACTTAAACC+AGG - chr5.2:4046911-4046930 MS.gene22581:CDS 35.0%
TTTACCATTCATGAGGATCA+TGG + chr5.2:4046447-4046466 None:intergenic 35.0%
! GATAATTCCATTGCCTTTTG+TGG - chr5.2:4046308-4046327 MS.gene22581:CDS 35.0%
! TCAGTGAATGTTATTTGAGC+AGG + chr5.2:4047265-4047284 None:intergenic 35.0%
! TGTAATGAGAGGATCTTTTC+TGG + chr5.2:4045516-4045535 None:intergenic 35.0%
!! AAACTTCAAGGTTTTGATCC+AGG - chr5.2:4046464-4046483 MS.gene22581:CDS 35.0%
!! ATTGCTGATTTAATAGCAGC+TGG + chr5.2:4046848-4046867 None:intergenic 35.0%
!! CCATATGATGATAATGGTCA+TGG - chr5.2:4045780-4045799 MS.gene22581:CDS 35.0%
!! GTAGCTTACTTTTCATCAAG+AGG - chr5.2:4046632-4046651 MS.gene22581:CDS 35.0%
!!! AGTGTTGATGCAAAGTCTTT+TGG + chr5.2:4046251-4046270 None:intergenic 35.0%
AAACCAGACATAATTGGACC+TGG - chr5.2:4046683-4046702 MS.gene22581:CDS 40.0%
AATAGGACCACAAAAGGCAA+TGG + chr5.2:4046318-4046337 None:intergenic 40.0%
AATGTCTATGGCAGTGCTAA+TGG - chr5.2:4045849-4045868 MS.gene22581:CDS 40.0%
ACAAGAAGATCATTGGAGCA+AGG - chr5.2:4045736-4045755 MS.gene22581:CDS 40.0%
ACACATGTAAGTTACTCTGC+TGG - chr5.2:4046515-4046534 MS.gene22581:CDS 40.0%
ACATAAAGTGAAATGCTCCG+AGG - chr5.2:4047078-4047097 MS.gene22581:CDS 40.0%
AGGGATCATAATTGGAATCC+TGG - chr5.2:4045608-4045627 MS.gene22581:CDS 40.0%
AGTGCTAATGGTACTGCAAT+AGG - chr5.2:4045861-4045880 MS.gene22581:CDS 40.0%
ATAATTGGAATCCTGGACAC+AGG - chr5.2:4045615-4045634 MS.gene22581:CDS 40.0%
ATAGACATTAGCACCTTGCA+CGG + chr5.2:4045838-4045857 None:intergenic 40.0%
CATGGAAATAGGACCACAAA+AGG + chr5.2:4046324-4046343 None:intergenic 40.0%
CCAGGTGATTTATTTGCAAC+TGG - chr5.2:4046929-4046948 MS.gene22581:CDS 40.0%
CCAGTTGCAAATAAATCACC+TGG + chr5.2:4046932-4046951 None:intergenic 40.0%
CCCAAAGACAAAGAGAATAG+AGG - chr5.2:4047322-4047341 MS.gene22581:CDS 40.0%
CTCGGTAAAGGGATCATAAT+TGG - chr5.2:4045600-4045619 MS.gene22581:CDS 40.0%
CTCTTTGTCTTTGGGTGTAA+TGG + chr5.2:4047317-4047336 None:intergenic 40.0%
GAACGTTGATGTTAAGGGAA+AGG - chr5.2:4046346-4046365 MS.gene22581:CDS 40.0%
GAGTAACTTACATGTGTAGC+AGG + chr5.2:4046512-4046531 None:intergenic 40.0%
GATTTATTTGCAACTGGTGC+TGG - chr5.2:4046935-4046954 MS.gene22581:CDS 40.0%
TAATGGTACTGCAATAGGCA+TGG - chr5.2:4045866-4045885 MS.gene22581:CDS 40.0%
TACATCTTCAAAGAAAGGGG+TGG + chr5.2:4046027-4046046 None:intergenic 40.0%
TAGATTGCCAAATGTGCTTC+TGG + chr5.2:4045894-4045913 None:intergenic 40.0%
TATGATCCCTTTACCGAGAT+TGG + chr5.2:4045598-4045617 None:intergenic 40.0%
TCAAGGTCATAAACAAGTCC+TGG + chr5.2:4046989-4047008 None:intergenic 40.0%
TTCTCAGGGATCTATCAAGT+GGG - chr5.2:4047357-4047376 MS.gene22581:CDS 40.0%
TTTCTCAGGGATCTATCAAG+TGG - chr5.2:4047356-4047375 MS.gene22581:CDS 40.0%
! AGTTGCTCAGCTGGAAATTT+TGG - chr5.2:4046092-4046111 MS.gene22581:CDS 40.0%
! ATGTCTGGTTTCAGAATTCC+TGG + chr5.2:4046674-4046693 None:intergenic 40.0%
! ATTTTCGTTAGTTGCTCAGC+TGG - chr5.2:4046083-4046102 MS.gene22581:CDS 40.0%
! TGTCTGGTTTCAGAATTCCT+GGG + chr5.2:4046673-4046692 None:intergenic 40.0%
!! TTCTTCTGGGGTCAATTTCA+CGG + chr5.2:4045451-4045470 None:intergenic 40.0%
!!! AAGGTAGTTTTGTGTGAGGA+AGG - chr5.2:4046365-4046384 MS.gene22581:CDS 40.0%
AACTCACACACCAAGCTTCT+TGG - chr5.2:4045539-4045558 MS.gene22581:CDS 45.0%
AAGTGCAGCATTAGCAGGAA+TGG - chr5.2:4045947-4045966 MS.gene22581:CDS 45.0%
ACAGAACCTGCATACACAAG+TGG + chr5.2:4046278-4046297 None:intergenic 45.0%
ACTCACACACCAAGCTTCTT+GGG - chr5.2:4045540-4045559 MS.gene22581:CDS 45.0%
ACTCCAGGTCCAATTATGTC+TGG + chr5.2:4046689-4046708 None:intergenic 45.0%
ACTGTGAATTCACAGGGGAA+AGG - chr5.2:4045706-4045725 MS.gene22581:CDS 45.0%
ACTTTCATCACAACGACCAC+TGG + chr5.2:4045931-4045950 None:intergenic 45.0%
AGAAAAGGAAGGATGGGAGA+GGG + chr5.2:4045646-4045665 None:intergenic 45.0%
ATCTTCAAAGAAAGGGGTGG+AGG + chr5.2:4046024-4046043 None:intergenic 45.0%
ATGTCTTGTCCTCATCTCAG+TGG - chr5.2:4046782-4046801 MS.gene22581:CDS 45.0%
ATTCCCTCGTCAGAAAAGGA+AGG + chr5.2:4045657-4045676 None:intergenic 45.0%
ATTGACCCCAGAAGAAGCAA+AGG - chr5.2:4045455-4045474 MS.gene22581:CDS 45.0%
CTAGAGCCTTTGCTTCTTCT+GGG + chr5.2:4045464-4045483 None:intergenic 45.0%
CTCGTCAGAAAAGGAAGGAT+GGG + chr5.2:4045652-4045671 None:intergenic 45.0%
CTCTGGTATGCTCTTTACCT+CGG + chr5.2:4047098-4047117 None:intergenic 45.0%
CTGGATCGTTTGCTTTCGAA+GGG + chr5.2:4046970-4046989 None:intergenic 45.0%
GAGATGAGGACAAGACATTG+AGG + chr5.2:4046780-4046799 None:intergenic 45.0%
GAGTGAACATTCTAGCAGCA+TGG - chr5.2:4046705-4046724 MS.gene22581:CDS 45.0%
GCACCAACAGTTAGAATCCA+CGG + chr5.2:4046149-4046168 None:intergenic 45.0%
GGATAATTTAGTTGCGCCTC+TGG + chr5.2:4047115-4047134 None:intergenic 45.0%
TACAAAGTATGCGCAACCAG+TGG - chr5.2:4045912-4045931 MS.gene22581:CDS 45.0%
TAGACATTAGCACCTTGCAC+GGG + chr5.2:4045837-4045856 None:intergenic 45.0%
TAGAGCCTTTGCTTCTTCTG+GGG + chr5.2:4045463-4045482 None:intergenic 45.0%
TCAGAATTCCTGGGCTTTCT+TGG + chr5.2:4046664-4046683 None:intergenic 45.0%
TCTCAACCTGCGAAATGGAA+CGG - chr5.2:4045681-4045700 MS.gene22581:CDS 45.0%
TCTTCAAAGAAAGGGGTGGA+GGG + chr5.2:4046023-4046042 None:intergenic 45.0%
TGCAACAGAGTCGAGGATTA+TGG - chr5.2:4045565-4045584 MS.gene22581:CDS 45.0%
TTTCCCCTGTGAATTCACAG+TGG + chr5.2:4045707-4045726 None:intergenic 45.0%
! CATCCTTCCTTTTCTGACGA+GGG - chr5.2:4045651-4045670 MS.gene22581:CDS 45.0%
! GATCCAGGTTCTGATGTTCA+GGG - chr5.2:4046479-4046498 MS.gene22581:CDS 45.0%
! GATGAAAGTGCAGCATTAGC+AGG - chr5.2:4045942-4045961 MS.gene22581:CDS 45.0%
! GGATCTATCAAGTGGGTTTC+TGG - chr5.2:4047364-4047383 MS.gene22581:CDS 45.0%
! GTGCAAGGTGCTAATGTCTA+TGG - chr5.2:4045837-4045856 MS.gene22581:CDS 45.0%
! TCTAGAGCCTTTGCTTCTTC+TGG + chr5.2:4045465-4045484 None:intergenic 45.0%
! TGATCCAGGTTCTGATGTTC+AGG - chr5.2:4046478-4046497 MS.gene22581:CDS 45.0%
! TTCCATTTCGCAGGTTGAGA+AGG + chr5.2:4045681-4045700 None:intergenic 45.0%
!! AAAGGCTCTAGAAGAGAAGG+AGG - chr5.2:4045473-4045492 MS.gene22581:CDS 45.0%
!! AGCAAAGGCTCTAGAAGAGA+AGG - chr5.2:4045470-4045489 MS.gene22581:CDS 45.0%
!!! GGGAAAGGTAGTTTTGTGTG+AGG - chr5.2:4046361-4046380 MS.gene22581:CDS 45.0%
!!! GTAGTTTTGTGTGAGGAAGG+TGG - chr5.2:4046368-4046387 MS.gene22581:CDS 45.0%
AACGGCCACTGTGAATTCAC+AGG - chr5.2:4045699-4045718 MS.gene22581:CDS 50.0%
ACGGCCACTGTGAATTCACA+GGG - chr5.2:4045700-4045719 MS.gene22581:CDS 50.0%
AGAGGGATATTCCTGTGTCC+AGG + chr5.2:4045629-4045648 None:intergenic 50.0%
AGCAGCATGGCATGCATCAT+TGG - chr5.2:4046718-4046737 MS.gene22581:CDS 50.0%
AGGCATTCCCTCGTCAGAAA+AGG + chr5.2:4045661-4045680 None:intergenic 50.0%
ATAGCAGCTGGAGACCAATC+TGG + chr5.2:4046836-4046855 None:intergenic 50.0%
ATGGACGCTGCAGTTGAAGA+CGG - chr5.2:4045966-4045985 MS.gene22581:CDS 50.0%
ATTCCTGGGCTTTCTTGGCT+TGG + chr5.2:4046659-4046678 None:intergenic 50.0%
CAAGCAGTTAAAGACGCTGG+TGG - chr5.2:4046416-4046435 MS.gene22581:CDS 50.0%
CAGAAAAGGAAGGATGGGAG+AGG + chr5.2:4045647-4045666 None:intergenic 50.0%
CCTCGTCAGAAAAGGAAGGA+TGG + chr5.2:4045653-4045672 None:intergenic 50.0%
CCTGGATCGTTTGCTTTCGA+AGG + chr5.2:4046971-4046990 None:intergenic 50.0%
CCTTCGAAAGCAAACGATCC+AGG - chr5.2:4046968-4046987 MS.gene22581:CDS 50.0%
CTCACACACCAAGCTTCTTG+GGG - chr5.2:4045541-4045560 MS.gene22581:CDS 50.0%
GCAGCAATTCCACTGAGATG+AGG + chr5.2:4046794-4046813 None:intergenic 50.0%
GCTCAAGCAGTTAAAGACGC+TGG - chr5.2:4046413-4046432 MS.gene22581:CDS 50.0%
GCTGCCATGATCCTCATGAA+TGG - chr5.2:4046440-4046459 MS.gene22581:CDS 50.0%
GCTGCTAGAATGTTCACTCC+AGG + chr5.2:4046704-4046723 None:intergenic 50.0%
TGCCTTCTCAACCTGCGAAA+TGG - chr5.2:4045676-4045695 MS.gene22581:CDS 50.0%
TGTTGCAACCCCAAGAAGCT+TGG + chr5.2:4045552-4045571 None:intergenic 50.0%
! CATGGCACCAGAAGCACATT+TGG - chr5.2:4045884-4045903 MS.gene22581:CDS 50.0%
! CCATCCTTCCTTTTCTGACG+AGG - chr5.2:4045650-4045669 MS.gene22581:CDS 50.0%
! CGAGGTAAAGAGCATACCAG+AGG - chr5.2:4047096-4047115 MS.gene22581:CDS 50.0%
! CTGTTGCCACTTGTGTATGC+AGG - chr5.2:4046269-4046288 MS.gene22581:CDS 50.0%
AAGGGGTGGAGGGTCCATTA+AGG + chr5.2:4046013-4046032 None:intergenic 55.0%
ACGCCCTGAACATCAGAACC+TGG + chr5.2:4046485-4046504 None:intergenic 55.0%
AGGGGTGGAGGGTCCATTAA+GGG + chr5.2:4046012-4046031 None:intergenic 55.0%
CGGCCACTGTGAATTCACAG+GGG - chr5.2:4045701-4045720 MS.gene22581:CDS 55.0%
GCGCCGTGGATTCTAACTGT+TGG - chr5.2:4046143-4046162 MS.gene22581:CDS 55.0%
TAAGCTACTTGAGGGGCGAG+TGG + chr5.2:4046620-4046639 None:intergenic 55.0%
TGGAACTCACACAGCCAGCA+CGG - chr5.2:4045800-4045819 MS.gene22581:CDS 55.0%
TTGGGGTTGCAACAGAGTCG+AGG - chr5.2:4045558-4045577 MS.gene22581:CDS 55.0%
!! CAACGTCTAATGAAGCGCCG+TGG - chr5.2:4046129-4046148 MS.gene22581:CDS 55.0%
!! GGCTCTAGAAGAGAAGGAGG+AGG - chr5.2:4045476-4045495 MS.gene22581:CDS 55.0%
CAGTGGCCGTTCCATTTCGC+AGG + chr5.2:4045690-4045709 None:intergenic 60.0%
GGACCAAGCCAAGAAAGCCC+AGG - chr5.2:4046653-4046672 MS.gene22581:CDS 60.0%
ACCTTGCACGGGCCTTCCAG+CGG + chr5.2:4045826-4045845 None:intergenic 65.0%
CACACAGCCAGCACGGCCGC+TGG - chr5.2:4045807-4045826 MS.gene22581:CDS 75.0%
CAGCCAGCACGGCCGCTGGA+AGG - chr5.2:4045811-4045830 MS.gene22581:CDS 75.0%
GCCGCTGGAAGGCCCGTGCA+AGG - chr5.2:4045822-4045841 MS.gene22581:CDS 75.0%
! GGGCCTTCCAGCGGCCGTGC+TGG + chr5.2:4045817-4045836 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 4045435 4047429 4045435 ID=MS.gene22581
chr5.2 mRNA 4045435 4047429 4045435 ID=MS.gene22581.t1;Parent=MS.gene22581
chr5.2 exon 4045435 4047429 4045435 ID=MS.gene22581.t1.exon1;Parent=MS.gene22581.t1
chr5.2 CDS 4045435 4047429 4045435 ID=cds.MS.gene22581.t1;Parent=MS.gene22581.t1
Gene Sequence

>MS.gene22581

ATGAATGGATTCGCCGTGAAATTGACCCCAGAAGAAGCAAAGGCTCTAGAAGAGAAGGAGGAGGTTCTATCAATTCGACCAGAAAAGATCCTCTCATTACATACAACTCACACACCAAGCTTCTTGGGGTTGCAACAGAGTCGAGGATTATGGAAAAATTCCAATCTCGGTAAAGGGATCATAATTGGAATCCTGGACACAGGAATATCCCTCTCCCATCCTTCCTTTTCTGACGAGGGAATGCCTTCTCAACCTGCGAAATGGAACGGCCACTGTGAATTCACAGGGGAAAGGATTTGTAACAAGAAGATCATTGGAGCAAGGACCTTAGTAAATTCATCCCTTCCATATGATGATAATGGTCATGGAACTCACACAGCCAGCACGGCCGCTGGAAGGCCCGTGCAAGGTGCTAATGTCTATGGCAGTGCTAATGGTACTGCAATAGGCATGGCACCAGAAGCACATTTGGCAATCTACAAAGTATGCGCAACCAGTGGTCGTTGTGATGAAAGTGCAGCATTAGCAGGAATGGACGCTGCAGTTGAAGACGGTGTTGATGTACTTTCAATCTCCCTTAATGGACCCTCCACCCCTTTCTTTGAAGATGTAATTGCACTTGGTGCATTTAGTGCAAATCAAAAGGGAATTTTCGTTAGTTGCTCAGCTGGAAATTTTGGTCCTGATTATATTACAACGTCTAATGAAGCGCCGTGGATTCTAACTGTTGGTGCTAGCACAACCGACAGAAAAATAGAAGCAATAGCTAAACTTAGCAATGGGGAAAAATATATTGGAGAATCAGTGTTTCAACCAAAAGACTTTGCATCAACACTGTTGCCACTTGTGTATGCAGGTTCTGTTAATTCAAGTGATAATTCCATTGCCTTTTGTGGTCCTATTTCCATGAAGAACGTTGATGTTAAGGGAAAGGTAGTTTTGTGTGAGGAAGGTGGACTTGTTTCACAAGCAGCAAAAGCTCAAGCAGTTAAAGACGCTGGTGGAGCTGCCATGATCCTCATGAATGGTAAACTTCAAGGTTTTGATCCAGGTTCTGATGTTCAGGGCGTTTTACCTGCTACACATGTAAGTTACTCTGCTGGTTTATCCATCAAAGATTATATAAACTCAACTTCAACACCAATGGCCACAATTTTATTCAAAGGAACTGTTATTGGAAATCCACTCGCCCCTCAAGTAGCTTACTTTTCATCAAGAGGACCAAGCCAAGAAAGCCCAGGAATTCTGAAACCAGACATAATTGGACCTGGAGTGAACATTCTAGCAGCATGGCATGCATCATTGGACAACAATATACCGCCATATAACATTATTTCAGGTACCTCAATGTCTTGTCCTCATCTCAGTGGAATTGCTGCTCTGTTGAAAAACTCTCATCCAGATTGGTCTCCAGCTGCTATTAAATCAGCAATTATGACAACTGCTTATGAGGTTAATCTTCAAGGAAAAGCTATATTAGACCAAAGACTTAAACCAGGTGATTTATTTGCAACTGGTGCTGGACATGTCAACCCTTCGAAAGCAAACGATCCAGGACTTGTTTATGACCTTGAACCAAATGATTATGTTCCTTATTTGTGTGGTTTAAATTATACTGATAGACAAGTTGGAATTATTCTGCAACATAAAGTGAAATGCTCCGAGGTAAAGAGCATACCAGAGGCGCAACTAAATTATCCATCATTTTCTATTTTTTTGGGGTCTACTTCTCAGTTTTATACTAGAACATTGACGAATGTTGGACCTGTCAACATGACTTACAATGTAGAAATTGATGTTCCTTTGGCAGTTGATATCACTATAAAGCCTGCTCAAATAACATTCACTGAGAAGAAGCAAAAAGTTACATACTCGGTTGCCATTACACCCAAAGACAAAGAGAATAGAGGTGATAACAAAATTTCTCAGGGATCTATCAAGTGGGTTTCTGGAAAGTATACCGTTAGAATACCTATATCTGTCATTTTTGTGTGA
Protein sequence

>MS.gene22581.t1

MNGFAVKLTPEEAKALEEKEEVLSIRPEKILSLHTTHTPSFLGLQQSRGLWKNSNLGKGIIIGILDTGISLSHPSFSDEGMPSQPAKWNGHCEFTGERICNKKIIGARTLVNSSLPYDDNGHGTHTASTAAGRPVQGANVYGSANGTAIGMAPEAHLAIYKVCATSGRCDESAALAGMDAAVEDGVDVLSISLNGPSTPFFEDVIALGAFSANQKGIFVSCSAGNFGPDYITTSNEAPWILTVGASTTDRKIEAIAKLSNGEKYIGESVFQPKDFASTLLPLVYAGSVNSSDNSIAFCGPISMKNVDVKGKVVLCEEGGLVSQAAKAQAVKDAGGAAMILMNGKLQGFDPGSDVQGVLPATHVSYSAGLSIKDYINSTSTPMATILFKGTVIGNPLAPQVAYFSSRGPSQESPGILKPDIIGPGVNILAAWHASLDNNIPPYNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTAYEVNLQGKAILDQRLKPGDLFATGAGHVNPSKANDPGLVYDLEPNDYVPYLCGLNYTDRQVGIILQHKVKCSEVKSIPEAQLNYPSFSIFLGSTSQFYTRTLTNVGPVNMTYNVEIDVPLAVDITIKPAQITFTEKKQKVTYSVAITPKDKENRGDNKISQGSIKWVSGKYTVRIPISVIFV