AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049656


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049656.t1 MTR_3g064020 84.615 247 35 1 1 244 1 247 3.40e-155 431
MS.gene049656.t1 MTR_5g076250 62.500 248 77 6 1 239 1 241 1.32e-95 280
MS.gene049656.t1 MTR_3g063990 54.980 251 71 4 9 221 3 249 8.25e-84 251
MS.gene049656.t1 MTR_1g057650 51.653 242 103 6 1 236 1 234 1.01e-73 225
MS.gene049656.t1 MTR_7g117750 43.983 241 121 5 1 236 1 232 1.74e-60 191
MS.gene049656.t1 MTR_3g063980 56.164 73 28 1 167 239 56 124 2.01e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049656.t1 AT3G02700 57.917 240 89 6 1 236 1 232 3.14e-81 244
MS.gene049656.t1 AT5G06370 50.826 242 105 6 1 236 1 234 8.77e-74 225
MS.gene049656.t1 AT5G16360 47.101 276 96 8 1 236 1 266 5.11e-70 216
MS.gene049656.t1 AT4G00905 45.714 245 120 5 1 236 1 241 5.87e-63 197
MS.gene049656.t1 AT5G16330 47.325 243 98 8 1 236 9 228 7.72e-62 194
MS.gene049656.t1 AT1G01225 47.391 230 112 3 1 226 1 225 1.82e-61 194

Find 56 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTAGATTGCTTTCTTTC+AGG 0.124117 3.2:-59561639 MS.gene049656:CDS
GCTTTATATCTTCTTGAAAA+TGG 0.152944 3.2:-59561501 MS.gene049656:CDS
AAAAGATTGGTTTCTGATAT+TGG 0.189791 3.2:-59561273 MS.gene049656:CDS
ATGTTTCCGATCAGTCTTAT+TGG 0.247318 3.2:+59562049 None:intergenic
CACAGTTATTCTTGAAGATA+TGG 0.269187 3.2:+59561466 None:intergenic
TATCTTCTTGAAAATGGATT+TGG 0.299009 3.2:-59561495 MS.gene049656:CDS
TTTGCAGTTTATTGCAAAAC+AGG 0.337490 3.2:-59561438 MS.gene049656:CDS
TGCATGTGCCTCCTCTAGCT+TGG 0.338272 3.2:+59561565 None:intergenic
CCGCACCGTCACGACATCTT+TGG 0.342473 3.2:+59561691 None:intergenic
GAACTCTACCTCTTTCAATA+TGG 0.373949 3.2:-59561612 MS.gene049656:CDS
AACTTACTGCATTAAAAGAT+TGG 0.377765 3.2:-59561286 MS.gene049656:CDS
ATGGTGATCCATTTCACAAT+TGG 0.385236 3.2:-59561792 MS.gene049656:CDS
CTGGGGATCATATTTACTCT+TGG 0.413378 3.2:-59562019 MS.gene049656:CDS
CTTCCATCCATGGTCAAATC+AGG 0.416092 3.2:+59561198 None:intergenic
GCATGTGCCTCCTCTAGCTT+GGG 0.419205 3.2:+59561566 None:intergenic
CACATGCACCCTAGCTGCTT+CGG 0.423293 3.2:-59561550 MS.gene049656:CDS
ATCCATTTCACAATTGGATC+AGG 0.464773 3.2:-59561786 MS.gene049656:CDS
ATCGGAAACATCTAAAACCT+GGG 0.479101 3.2:-59562037 MS.gene049656:CDS
GGGATCATATTTACTCTTGG+AGG 0.483005 3.2:-59562016 MS.gene049656:CDS
ACAGGAATATATGTTGAAGA+TGG 0.483154 3.2:-59561816 MS.gene049656:intron
GTCCTGATCCAATTGTGAAA+TGG 0.487607 3.2:+59561784 None:intergenic
GGATCAGGACAGCAAGCTGC+TGG 0.487944 3.2:-59561771 MS.gene049656:CDS
CGAAAGAACTTGTAAAGAAA+CGG 0.498819 3.2:+59561730 None:intergenic
TCATATTTACTCTTGGAGGC+AGG 0.503930 3.2:-59562012 MS.gene049656:CDS
ATACCTGATTTGACCATGGA+TGG 0.505525 3.2:-59561201 MS.gene049656:CDS
GCTGGCAGCAGCTGAATAAG+AGG 0.511661 3.2:+59561377 None:intergenic
TGAAAGAAAGCAATCTAAGC+AGG 0.512899 3.2:+59561641 None:intergenic
ACATCTTTGGCATGGAATGT+TGG 0.518788 3.2:+59561704 None:intergenic
TTCAAGAAGATATAAAGCAC+GGG 0.519285 3.2:+59561506 None:intergenic
TGGAGACACCATATTGAAAG+AGG 0.529495 3.2:+59561604 None:intergenic
CAGTTGGATCCGAAGCAGCT+AGG 0.530992 3.2:+59561541 None:intergenic
AGTTGGATCCGAAGCAGCTA+GGG 0.533238 3.2:+59561542 None:intergenic
GTCGTGACGGTGCGGAAACA+AGG 0.535193 3.2:-59561683 MS.gene049656:CDS
AGTCTACTAGCTTCCATCCA+TGG 0.540139 3.2:+59561188 None:intergenic
GATCGGAAACATCTAAAACC+TGG 0.543021 3.2:-59562038 MS.gene049656:CDS
AAGAAACGGTATAAAATCGC+TGG 0.543391 3.2:+59561744 None:intergenic
TTTCAAGAAGATATAAAGCA+CGG 0.545122 3.2:+59561505 None:intergenic
CGGGGAAGGACCTCTTCAGT+TGG 0.547650 3.2:+59561525 None:intergenic
GGTAGGGCTTTGGTTTGCTG+TGG 0.552956 3.2:-59561309 MS.gene049656:CDS
TCAACCTCAAAAGGCTGCAG+TGG 0.553525 3.2:-59561408 MS.gene049656:CDS
TTAGATTGCTTTCTTTCAGG+AGG 0.554330 3.2:-59561636 MS.gene049656:CDS
TGCTTTCCAATAAGACTGAT+CGG 0.558642 3.2:-59562055 MS.gene049656:CDS
TGATGAAGAGTCTACAGTGC+TGG 0.563974 3.2:+59561359 None:intergenic
CAAGAGTAAATATGATCCCC+AGG 0.568302 3.2:+59562020 None:intergenic
CTGTAGACTCTTCATCACTT+CGG 0.584097 3.2:-59561353 MS.gene049656:CDS
CCGTCACGACATCTTTGGCA+TGG 0.598030 3.2:+59561696 None:intergenic
GAAGATATAAAGCACGGGGA+AGG 0.600493 3.2:+59561511 None:intergenic
AATATATGTTGAAGATGGAA+TGG 0.601381 3.2:-59561811 MS.gene049656:CDS
GCTTACCTCTATGCACATCA+TGG 0.604488 3.2:-59561990 MS.gene049656:intron
GGTGCGGAAACAAGGACTCA+TGG 0.614117 3.2:-59561675 MS.gene049656:CDS
GATGACCATGATGTGCATAG+AGG 0.641641 3.2:+59561985 None:intergenic
TGCTTCGGATCCAACTGAAG+AGG 0.647462 3.2:-59561535 MS.gene049656:CDS
CCAAAGATGTCGTGACGGTG+CGG 0.654870 3.2:-59561691 MS.gene049656:CDS
CCATGCCAAAGATGTCGTGA+CGG 0.658966 3.2:-59561696 MS.gene049656:CDS
TCAAGAAGATATAAAGCACG+GGG 0.677622 3.2:+59561507 None:intergenic
TCGGAAACATCTAAAACCTG+GGG 0.712111 3.2:-59562036 MS.gene049656:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTTGATACATAAGAAAA+TGG + chr3.2:59561349-59561368 None:intergenic 20.0%
! AACTTACTGCATTAAAAGAT+TGG - chr3.2:59561970-59561989 MS.gene049656:intron 25.0%
! AATATATGTTGAAGATGGAA+TGG - chr3.2:59561445-59561464 MS.gene049656:CDS 25.0%
! GCTTTATATCTTCTTGAAAA+TGG - chr3.2:59561755-59561774 MS.gene049656:CDS 25.0%
! TTTCAAGAAGATATAAAGCA+CGG + chr3.2:59561754-59561773 None:intergenic 25.0%
!! TATCTTCTTGAAAATGGATT+TGG - chr3.2:59561761-59561780 MS.gene049656:CDS 25.0%
!! TTGTGCAAGAAAGTATAAAT+TGG + chr3.2:59561319-59561338 None:intergenic 25.0%
!!! AAAAGATTGGTTTCTGATAT+TGG - chr3.2:59561983-59562002 MS.gene049656:intron 25.0%
!!! AAATCAGGTATTTTTTCAAC+TGG + chr3.2:59562046-59562065 None:intergenic 25.0%
ACAGGAATATATGTTGAAGA+TGG - chr3.2:59561440-59561459 MS.gene049656:CDS 30.0%
CACAGTTATTCTTGAAGATA+TGG + chr3.2:59561793-59561812 None:intergenic 30.0%
CGAAAGAACTTGTAAAGAAA+CGG + chr3.2:59561529-59561548 None:intergenic 30.0%
TCAGTATGATAGTTATGCAT+AGG + chr3.2:59561374-59561393 None:intergenic 30.0%
TTCAAGAAGATATAAAGCAC+GGG + chr3.2:59561753-59561772 None:intergenic 30.0%
TTGGGCTAAAAAATGTAGTT+TGG + chr3.2:59561675-59561694 None:intergenic 30.0%
TTTGCAGTTTATTGCAAAAC+AGG - chr3.2:59561818-59561837 MS.gene049656:intron 30.0%
! AAAAATACCTGATTTGACCA+TGG - chr3.2:59562051-59562070 MS.gene049656:CDS 30.0%
AAGAAACGGTATAAAATCGC+TGG + chr3.2:59561515-59561534 None:intergenic 35.0%
ATCCATTTCACAATTGGATC+AGG - chr3.2:59561470-59561489 MS.gene049656:CDS 35.0%
ATGGTGATCCATTTCACAAT+TGG - chr3.2:59561464-59561483 MS.gene049656:CDS 35.0%
CGGCATTTCTGTAATATAAC+AGG - chr3.2:59561422-59561441 MS.gene049656:CDS 35.0%
GAACTCTACCTCTTTCAATA+TGG - chr3.2:59561644-59561663 MS.gene049656:CDS 35.0%
TCAAGAAGATATAAAGCACG+GGG + chr3.2:59561752-59561771 None:intergenic 35.0%
TCATATTTGTGTCCTGCTAA+CGG - chr3.2:59561402-59561421 MS.gene049656:CDS 35.0%
TGAAAGAAAGCAATCTAAGC+AGG + chr3.2:59561618-59561637 None:intergenic 35.0%
TGCTTAGATTGCTTTCTTTC+AGG - chr3.2:59561617-59561636 MS.gene049656:CDS 35.0%
TGCTTTCCAATAAGACTGAT+CGG - chr3.2:59561201-59561220 MS.gene049656:CDS 35.0%
TTAGATTGCTTTCTTTCAGG+AGG - chr3.2:59561620-59561639 MS.gene049656:CDS 35.0%
! ATCGGAAACATCTAAAACCT+GGG - chr3.2:59561219-59561238 MS.gene049656:CDS 35.0%
! ATGTTTCCGATCAGTCTTAT+TGG + chr3.2:59561210-59561229 None:intergenic 35.0%
! TTTGTGAACTCCAGTCTTTT+TGG - chr3.2:59561926-59561945 MS.gene049656:intron 35.0%
!!! GGTCTTTTTTCAACCTCAAA+AGG - chr3.2:59561839-59561858 MS.gene049656:intron 35.0%
ACATCTTTGGCATGGAATGT+TGG + chr3.2:59561555-59561574 None:intergenic 40.0%
ATTACAGAAATGCCGTTAGC+AGG + chr3.2:59561417-59561436 None:intergenic 40.0%
CAAGAGTAAATATGATCCCC+AGG + chr3.2:59561239-59561258 None:intergenic 40.0%
CTGGGGATCATATTTACTCT+TGG - chr3.2:59561237-59561256 MS.gene049656:CDS 40.0%
CTGTAGACTCTTCATCACTT+CGG - chr3.2:59561903-59561922 MS.gene049656:intron 40.0%
GGGATCATATTTACTCTTGG+AGG - chr3.2:59561240-59561259 MS.gene049656:CDS 40.0%
GTCCTGATCCAATTGTGAAA+TGG + chr3.2:59561475-59561494 None:intergenic 40.0%
TCATATTTACTCTTGGAGGC+AGG - chr3.2:59561244-59561263 MS.gene049656:CDS 40.0%
TGGAGACACCATATTGAAAG+AGG + chr3.2:59561655-59561674 None:intergenic 40.0%
! ATACCTGATTTGACCATGGA+TGG - chr3.2:59562055-59562074 MS.gene049656:CDS 40.0%
! GATCGGAAACATCTAAAACC+TGG - chr3.2:59561218-59561237 MS.gene049656:CDS 40.0%
! TCGGAAACATCTAAAACCTG+GGG - chr3.2:59561220-59561239 MS.gene049656:CDS 40.0%
!! GAACTCCAGTCTTTTTGGTA+GGG - chr3.2:59561931-59561950 MS.gene049656:intron 40.0%
!! TGAACTCCAGTCTTTTTGGT+AGG - chr3.2:59561930-59561949 MS.gene049656:intron 40.0%
!!! CATTTTTTAGCCCAAGCTAG+AGG - chr3.2:59561680-59561699 MS.gene049656:CDS 40.0%
CAAAGCCCTACCAAAAAGAC+TGG + chr3.2:59561939-59561958 None:intergenic 45.0%
CTTCCATCCATGGTCAAATC+AGG + chr3.2:59562061-59562080 None:intergenic 45.0%
GAAGATATAAAGCACGGGGA+AGG + chr3.2:59561748-59561767 None:intergenic 45.0%
GATGACCATGATGTGCATAG+AGG + chr3.2:59561274-59561293 None:intergenic 45.0%
GCTTACCTCTATGCACATCA+TGG - chr3.2:59561266-59561285 MS.gene049656:CDS 45.0%
TGATGAAGAGTCTACAGTGC+TGG + chr3.2:59561900-59561919 None:intergenic 45.0%
!! CAGTCTTTTTGGTAGGGCTT+TGG - chr3.2:59561937-59561956 MS.gene049656:intron 45.0%
!! TTTTTAGCCCAAGCTAGAGG+AGG - chr3.2:59561683-59561702 MS.gene049656:CDS 45.0%
AGTTGGATCCGAAGCAGCTA+GGG + chr3.2:59561717-59561736 None:intergenic 50.0%
CCATGCCAAAGATGTCGTGA+CGG - chr3.2:59561560-59561579 MS.gene049656:CDS 50.0%
TCAACCTCAAAAGGCTGCAG+TGG - chr3.2:59561848-59561867 MS.gene049656:intron 50.0%
TGCTTCGGATCCAACTGAAG+AGG - chr3.2:59561721-59561740 MS.gene049656:CDS 50.0%
! TTGACCACTGCAGCCTTTTG+AGG + chr3.2:59561855-59561874 None:intergenic 50.0%
CACATGCACCCTAGCTGCTT+CGG - chr3.2:59561706-59561725 MS.gene049656:CDS 55.0%
CAGTTGGATCCGAAGCAGCT+AGG + chr3.2:59561718-59561737 None:intergenic 55.0%
CCAAAGATGTCGTGACGGTG+CGG - chr3.2:59561565-59561584 MS.gene049656:CDS 55.0%
CCGTCACGACATCTTTGGCA+TGG + chr3.2:59561563-59561582 None:intergenic 55.0%
GCTGGCAGCAGCTGAATAAG+AGG + chr3.2:59561882-59561901 None:intergenic 55.0%
GGTGCGGAAACAAGGACTCA+TGG - chr3.2:59561581-59561600 MS.gene049656:CDS 55.0%
TGCATGTGCCTCCTCTAGCT+TGG + chr3.2:59561694-59561713 None:intergenic 55.0%
! GCATGTGCCTCCTCTAGCTT+GGG + chr3.2:59561693-59561712 None:intergenic 55.0%
!! GGTAGGGCTTTGGTTTGCTG+TGG - chr3.2:59561947-59561966 MS.gene049656:intron 55.0%
CCGCACCGTCACGACATCTT+TGG + chr3.2:59561568-59561587 None:intergenic 60.0%
CGGGGAAGGACCTCTTCAGT+TGG + chr3.2:59561734-59561753 None:intergenic 60.0%
GGATCAGGACAGCAAGCTGC+TGG - chr3.2:59561485-59561504 MS.gene049656:CDS 60.0%
GTCGTGACGGTGCGGAAACA+AGG - chr3.2:59561573-59561592 MS.gene049656:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 59561194 59562084 59561194 ID=MS.gene049656
chr3.2 mRNA 59561194 59562084 59561194 ID=MS.gene049656.t1;Parent=MS.gene049656
chr3.2 exon 59561991 59562084 59561991 ID=MS.gene049656.t1.exon1;Parent=MS.gene049656.t1
chr3.2 CDS 59561991 59562084 59561991 ID=cds.MS.gene049656.t1;Parent=MS.gene049656.t1
chr3.2 exon 59561194 59561834 59561194 ID=MS.gene049656.t1.exon2;Parent=MS.gene049656.t1
chr3.2 CDS 59561194 59561834 59561194 ID=cds.MS.gene049656.t1;Parent=MS.gene049656.t1
Gene Sequence

>MS.gene049656

ATGGGAGTGCTTTCCAATAAGACTGATCGGAAACATCTAAAACCTGGGGATCATATTTACTCTTGGAGGCAGGCTTACCTCTATGCACATCATGGTCATCTCTTTTCTCTTATATTTATCTGCCAATTTATACTTTCTTGCACAACTATGAACCATTTTCTTATGTATCAAACTTATCCTATGCATAACTATCATACTGATGTTAATGTCATATTTGTGTCCTGCTAACGGCATTTCTGTAATATAACAGGAATATATGTTGAAGATGGAATGGTGATCCATTTCACAATTGGATCAGGACAGCAAGCTGCTGGAACACCAGCGATTTTATACCGTTTCTTTACAAGTTCTTTCGATGCCAACATTCCATGCCAAAGATGTCGTGACGGTGCGGAAACAAGGACTCATGGTGTCTTCTCATCCTGCTTAGATTGCTTTCTTTCAGGAGGTGAACTCTACCTCTTTCAATATGGTGTCTCCAAACTACATTTTTTAGCCCAAGCTAGAGGAGGCACATGCACCCTAGCTGCTTCGGATCCAACTGAAGAGGTCCTTCCCCGTGCTTTATATCTTCTTGAAAATGGATTTGGTGACTACCATATCTTCAAGAATAACTGTGAAGATTTTGCAGTTTATTGCAAAACAGGTCTTTTTTCAACCTCAAAAGGCTGCAGTGGTCAAGCAGCCTCTTATTCAGCTGCTGCCAGCACTGTAGACTCTTCATCACTTCGGTTTGTGAACTCCAGTCTTTTTGGTAGGGCTTTGGTTTGCTGTGGAACTTACTGCATTAAAAGATTGGTTTCTGATATTGGATTTCGTTATGAAGTAATTAAAGATGTAACTAAAGTTCCAGTTGAAAAAATACCTGATTTGACCATGGATGGAAGCTAG

Protein sequence

>MS.gene049656.t1

MGVLSNKTDRKHLKPGDHIYSWRQAYLYAHHGIYVEDGMVIHFTIGSGQQAAGTPAILYRFFTSSFDANIPCQRCRDGAETRTHGVFSSCLDCFLSGGELYLFQYGVSKLHFLAQARGGTCTLAASDPTEEVLPRALYLLENGFGDYHIFKNNCEDFAVYCKTGLFSTSKGCSGQAASYSAAASTVDSSSLRFVNSSLFGRALVCCGTYCIKRLVSDIGFRYEVIKDVTKVPVEKIPDLTMDGS