Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23306.t1 | XP_003606964.1 | 95.6 | 225 | 10 | 0 | 1 | 225 | 1 | 225 | 2.00E-109 | 405.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23306.t1 | G7JHF1 | 95.6 | 225 | 10 | 0 | 1 | 225 | 1 | 225 | 1.4e-109 | 405.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene23306 | 0.81406 | 1.87E-51 | -1.69E-46 |
| MS.gene050680 | MS.gene23306 | 0.802262 | 6.10E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene23306 | 0.816195 | 6.27E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene23306 | 0.810404 | 1.17E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene23306 | 0.80646 | 8.14E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene23306 | 0.801661 | 8.10E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene23306 | 0.808373 | 3.20E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene23306 | 0.813791 | 2.14E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene23306 | 0.817566 | 3.09E-52 | -1.69E-46 |
| MS.gene054183 | MS.gene23306 | 0.803827 | 2.89E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene23306 | 0.803199 | 3.91E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene23306 | 0.80995 | 1.47E-50 | -1.69E-46 |
| MS.gene054559 | MS.gene23306 | 0.823219 | 1.56E-53 | -1.69E-46 |
| MS.gene054713 | MS.gene23306 | 0.81151 | 6.75E-51 | -1.69E-46 |
| MS.gene054983 | MS.gene23306 | 0.819638 | 1.05E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene23306 | 0.828937 | 6.81E-55 | -1.69E-46 |
| MS.gene055358 | MS.gene23306 | 0.836449 | 9.30E-57 | -1.69E-46 |
| MS.gene055701 | MS.gene23306 | 0.819781 | 9.70E-53 | -1.69E-46 |
| MS.gene057639 | MS.gene23306 | 0.942591 | 5.48E-102 | -1.69E-46 |
| MS.gene059128 | MS.gene23306 | 0.821673 | 3.56E-53 | -1.69E-46 |
| MS.gene061114 | MS.gene23306 | -0.851644 | 7.76E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23306.t1 | MTR_4g070660 | 95.556 | 225 | 10 | 0 | 1 | 225 | 1 | 225 | 2.83e-146 | 407 |
| MS.gene23306.t1 | MTR_4g070640 | 72.566 | 226 | 48 | 5 | 1 | 225 | 1 | 213 | 7.71e-94 | 273 |
| MS.gene23306.t1 | MTR_4g070630 | 65.000 | 180 | 52 | 3 | 46 | 225 | 1 | 169 | 6.79e-72 | 216 |
| MS.gene23306.t1 | MTR_3g117050 | 37.984 | 258 | 101 | 12 | 9 | 221 | 10 | 253 | 4.64e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23306.t1 | AT5G66440 | 30.705 | 241 | 141 | 8 | 1 | 220 | 1 | 236 | 3.01e-23 | 94.4 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCGTGTTCCTGTTCTTT+TGG | 0.253242 | 4.3:+34504344 | None:intergenic |
| CGTTGAAGTGTGTGTTGTTT+TGG | 0.285321 | 4.3:-34504528 | MS.gene23306:CDS |
| CAAACAACGTGATGATTATA+AGG | 0.288441 | 4.3:+34504390 | None:intergenic |
| AAACTCGAGCGAGTATTTAA+AGG | 0.299130 | 4.3:-34504462 | MS.gene23306:CDS |
| CTCGAGTTTCTTACCACAAT+TGG | 0.332741 | 4.3:+34504476 | None:intergenic |
| GAGAAAATGAAAACTAAAAC+AGG | 0.335030 | 4.3:-34504203 | MS.gene23306:CDS |
| TGCTGCTGGTGAGGAGAAAA+TGG | 0.340169 | 4.3:-34504063 | MS.gene23306:CDS |
| AGAAGGGTTATTGTTATAGA+AGG | 0.356480 | 4.3:-34504175 | MS.gene23306:CDS |
| TTGCGTTGGCTTTCTCTGCT+TGG | 0.357630 | 4.3:+34504597 | None:intergenic |
| TCATAGAAAGCCTTGAAATC+AGG | 0.416707 | 4.3:+34504504 | None:intergenic |
| TTCTATGAGGTTACCAATTG+TGG | 0.435111 | 4.3:-34504489 | MS.gene23306:CDS |
| CGATGATATTTATGAAAAGT+TGG | 0.442726 | 4.3:-34504327 | MS.gene23306:CDS |
| CGTTGGCTTTCTCTGCTTGG+AGG | 0.453384 | 4.3:+34504600 | None:intergenic |
| GATATTTATGAAAAGTTGGC+AGG | 0.457338 | 4.3:-34504323 | MS.gene23306:CDS |
| TAAAACATCATAAGCTTCAA+AGG | 0.465357 | 4.3:-34504571 | MS.gene23306:CDS |
| AAAGGGCAACAACTTTGTTA+CGG | 0.468035 | 4.3:-34504553 | MS.gene23306:CDS |
| AGCTGCTCCTGCTGCTGGTG+AGG | 0.474801 | 4.3:-34504072 | MS.gene23306:CDS |
| ATTGAAGCTGCTCCTGCTGC+TGG | 0.500424 | 4.3:-34504077 | MS.gene23306:CDS |
| AACTAAAACAGGGTTGAAGA+AGG | 0.504135 | 4.3:-34504192 | MS.gene23306:CDS |
| AAGCAACAAAGAGGATTTGA+GGG | 0.514889 | 4.3:-34503969 | MS.gene23306:CDS |
| AAAACATCATAAGCTTCAAA+GGG | 0.531611 | 4.3:-34504570 | MS.gene23306:CDS |
| AATGGTGAGAATTTCGTATC+CGG | 0.535348 | 4.3:-34504045 | MS.gene23306:CDS |
| AAAGCAACAAAGAGGATTTG+AGG | 0.536464 | 4.3:-34503970 | MS.gene23306:CDS |
| ATCATCACGTTGTTTGCTCA+AGG | 0.539246 | 4.3:-34504383 | MS.gene23306:CDS |
| AAATATGATAGCACAAAAGA+AGG | 0.540013 | 4.3:-34504263 | MS.gene23306:CDS |
| TCGTTGCTCATCTCATCCTC+CGG | 0.543243 | 4.3:+34504026 | None:intergenic |
| TTTATTGCAAAGCAACAAAG+AGG | 0.547495 | 4.3:-34503978 | MS.gene23306:CDS |
| TGATTTCAAGGCTTTCTATG+AGG | 0.550039 | 4.3:-34504502 | MS.gene23306:CDS |
| ACTAAAACAGGGTTGAAGAA+GGG | 0.598384 | 4.3:-34504191 | MS.gene23306:CDS |
| GGAGTTTCGTCGTACTGTAG+AGG | 0.600879 | 4.3:-34504003 | MS.gene23306:CDS |
| GTGAGAGTGAGAACAGCGGA+AGG | 0.613601 | 4.3:-34504106 | MS.gene23306:CDS |
| GAACAAGAGAATTGATGATG+CGG | 0.644326 | 4.3:-34504228 | MS.gene23306:CDS |
| GGTGAGAATTTCGTATCCGG+AGG | 0.672479 | 4.3:-34504042 | MS.gene23306:CDS |
| AGATGTGAGAGTGAGAACAG+CGG | 0.688021 | 4.3:-34504110 | MS.gene23306:CDS |
| AGAAAATGAAAACTAAAACA+GGG | 0.723999 | 4.3:-34504202 | MS.gene23306:CDS |
| AGCAACAAAGAGGATTTGAG+GGG | 0.729509 | 4.3:-34503968 | MS.gene23306:CDS |
| CGATAGCGAAGACAAAACAG+TGG | 0.743095 | 4.3:+34504417 | None:intergenic |
| GGAGGATGAGATGAGCAACG+AGG | 0.778929 | 4.3:-34504024 | MS.gene23306:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAAATGAAAACTAAAACA+GGG | - | chr4.3:34504383-34504402 | MS.gene23306:CDS | 20.0% |
| ! | AAAAAACATTCCAAAAGAAC+AGG | - | chr4.3:34504231-34504250 | MS.gene23306:CDS | 25.0% |
| ! | AAAACATCATAAGCTTCAAA+GGG | - | chr4.3:34504015-34504034 | MS.gene23306:CDS | 25.0% |
| ! | AAATATGATAGCACAAAAGA+AGG | - | chr4.3:34504322-34504341 | MS.gene23306:CDS | 25.0% |
| ! | CGATGATATTTATGAAAAGT+TGG | - | chr4.3:34504258-34504277 | MS.gene23306:CDS | 25.0% |
| ! | GAGAAAATGAAAACTAAAAC+AGG | - | chr4.3:34504382-34504401 | MS.gene23306:CDS | 25.0% |
| ! | TAAAACATCATAAGCTTCAA+AGG | - | chr4.3:34504014-34504033 | MS.gene23306:CDS | 25.0% |
| !!! | AAACTGAGATTTTGAAGAAA+CGG | - | chr4.3:34504437-34504456 | MS.gene23306:CDS | 25.0% |
| !!! | TTTGAAGCTTATGATGTTTT+AGG | + | chr4.3:34504015-34504034 | None:intergenic | 25.0% |
| AGAAGGGTTATTGTTATAGA+AGG | - | chr4.3:34504410-34504429 | MS.gene23306:CDS | 30.0% | |
| CAAACAACGTGATGATTATA+AGG | + | chr4.3:34504198-34504217 | None:intergenic | 30.0% | |
| GATATTTATGAAAAGTTGGC+AGG | - | chr4.3:34504262-34504281 | MS.gene23306:CDS | 30.0% | |
| TTTATTGCAAAGCAACAAAG+AGG | - | chr4.3:34504607-34504626 | MS.gene23306:CDS | 30.0% | |
| AAACTCGAGCGAGTATTTAA+AGG | - | chr4.3:34504123-34504142 | MS.gene23306:CDS | 35.0% | |
| AAAGCAACAAAGAGGATTTG+AGG | - | chr4.3:34504615-34504634 | MS.gene23306:CDS | 35.0% | |
| AAAGGGCAACAACTTTGTTA+CGG | - | chr4.3:34504032-34504051 | MS.gene23306:CDS | 35.0% | |
| AACTAAAACAGGGTTGAAGA+AGG | - | chr4.3:34504393-34504412 | MS.gene23306:CDS | 35.0% | |
| AAGCAACAAAGAGGATTTGA+GGG | - | chr4.3:34504616-34504635 | MS.gene23306:CDS | 35.0% | |
| AATGGTGAGAATTTCGTATC+CGG | - | chr4.3:34504540-34504559 | MS.gene23306:CDS | 35.0% | |
| ACTAAAACAGGGTTGAAGAA+GGG | - | chr4.3:34504394-34504413 | MS.gene23306:CDS | 35.0% | |
| TCATAGAAAGCCTTGAAATC+AGG | + | chr4.3:34504084-34504103 | None:intergenic | 35.0% | |
| TGATTTCAAGGCTTTCTATG+AGG | - | chr4.3:34504083-34504102 | MS.gene23306:CDS | 35.0% | |
| TTCTATGAGGTTACCAATTG+TGG | - | chr4.3:34504096-34504115 | MS.gene23306:CDS | 35.0% | |
| !! | GAACAAGAGAATTGATGATG+CGG | - | chr4.3:34504357-34504376 | MS.gene23306:CDS | 35.0% |
| !!! | ATGATGTTTTAGGATTGCGT+TGG | + | chr4.3:34504005-34504024 | None:intergenic | 35.0% |
| !!! | TTGTTTTGGTCCTGATTTCA+AGG | - | chr4.3:34504071-34504090 | MS.gene23306:CDS | 35.0% |
| AGCAACAAAGAGGATTTGAG+GGG | - | chr4.3:34504617-34504636 | MS.gene23306:CDS | 40.0% | |
| ATCATCACGTTGTTTGCTCA+AGG | - | chr4.3:34504202-34504221 | MS.gene23306:CDS | 40.0% | |
| CTCGAGTTTCTTACCACAAT+TGG | + | chr4.3:34504112-34504131 | None:intergenic | 40.0% | |
| ! | CACTGTTTTGTCTTCGCTAT+CGG | - | chr4.3:34504169-34504188 | MS.gene23306:CDS | 40.0% |
| ! | TCATCGTGTTCCTGTTCTTT+TGG | + | chr4.3:34504244-34504263 | None:intergenic | 40.0% |
| !!! | CGTTGAAGTGTGTGTTGTTT+TGG | - | chr4.3:34504057-34504076 | MS.gene23306:CDS | 40.0% |
| AGATGTGAGAGTGAGAACAG+CGG | - | chr4.3:34504475-34504494 | MS.gene23306:CDS | 45.0% | |
| CGATAGCGAAGACAAAACAG+TGG | + | chr4.3:34504171-34504190 | None:intergenic | 45.0% | |
| GGAGTTTCGTCGTACTGTAG+AGG | - | chr4.3:34504582-34504601 | MS.gene23306:CDS | 50.0% | |
| GGTGAGAATTTCGTATCCGG+AGG | - | chr4.3:34504543-34504562 | MS.gene23306:CDS | 50.0% | |
| TCGTTGCTCATCTCATCCTC+CGG | + | chr4.3:34504562-34504581 | None:intergenic | 50.0% | |
| TGCTGCTGGTGAGGAGAAAA+TGG | - | chr4.3:34504522-34504541 | MS.gene23306:CDS | 50.0% | |
| ! | ATTTTCTCCTCACCAGCAGC+AGG | + | chr4.3:34504523-34504542 | None:intergenic | 50.0% |
| !! | TTGCGTTGGCTTTCTCTGCT+TGG | + | chr4.3:34503991-34504010 | None:intergenic | 50.0% |
| ATTGAAGCTGCTCCTGCTGC+TGG | - | chr4.3:34504508-34504527 | MS.gene23306:CDS | 55.0% | |
| GGAGGATGAGATGAGCAACG+AGG | - | chr4.3:34504561-34504580 | MS.gene23306:CDS | 55.0% | |
| GTGAGAGTGAGAACAGCGGA+AGG | - | chr4.3:34504479-34504498 | MS.gene23306:CDS | 55.0% | |
| !! | CGTTGGCTTTCTCTGCTTGG+AGG | + | chr4.3:34503988-34504007 | None:intergenic | 55.0% |
| AGCTGCTCCTGCTGCTGGTG+AGG | - | chr4.3:34504513-34504532 | MS.gene23306:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 34503965 | 34504642 | 34503965 | ID=MS.gene23306 |
| chr4.3 | mRNA | 34503965 | 34504642 | 34503965 | ID=MS.gene23306.t1;Parent=MS.gene23306 |
| chr4.3 | exon | 34503965 | 34504642 | 34503965 | ID=MS.gene23306.t1.exon1;Parent=MS.gene23306.t1 |
| chr4.3 | CDS | 34503965 | 34504642 | 34503965 | ID=cds.MS.gene23306.t1;Parent=MS.gene23306.t1 |
| Gene Sequence |
| Protein sequence |
