AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057639


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057639.t1 MTR_4g070660 96.000 225 9 0 1 225 1 225 7.10e-147 408
MS.gene057639.t1 MTR_4g070640 73.009 226 47 5 1 225 1 213 2.34e-94 275
MS.gene057639.t1 MTR_4g070630 65.000 180 52 3 46 225 1 169 3.80e-72 217
MS.gene057639.t1 MTR_3g117050 37.984 258 101 12 9 221 10 253 1.86e-32 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057639.t1 AT5G66440 30.705 241 141 8 1 220 1 236 1.23e-23 95.5
MS.gene057639.t1 AT4G34560 30.851 188 86 9 52 221 50 211 8.90e-11 60.1

Find 38 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCATGTTCCTGTTCTTT+TGG 0.223580 4.2:-36472144 None:intergenic
CGTTGAAGTGTGTGTTGTTT+TGG 0.285321 4.2:+36471960 MS.gene057639:CDS
CAAACAACGTGATGATTATA+AGG 0.288441 4.2:-36472098 None:intergenic
AAACTCGAGCGAGTATTTAA+AGG 0.299130 4.2:+36472026 MS.gene057639:CDS
CTCGAGTTTCTTACCACAAT+TGG 0.332741 4.2:-36472012 None:intergenic
GAGAAAATGAAAACTAAAAC+AGG 0.335030 4.2:+36472285 MS.gene057639:CDS
TGCTGCTGGTGAGGAGAAAA+TGG 0.340169 4.2:+36472425 MS.gene057639:CDS
AGAAGGGTTATTGTTATAGA+AGG 0.356480 4.2:+36472313 MS.gene057639:CDS
TTGCGTTGGCTTTCTCTGCT+TGG 0.357630 4.2:-36471891 None:intergenic
TCATAGAAAGCCTTGAAATC+AGG 0.416707 4.2:-36471984 None:intergenic
TTCTATGAGGTTACCAATTG+TGG 0.435111 4.2:+36471999 MS.gene057639:CDS
CGTTGGCTTTCTCTGCTTGG+AGG 0.453384 4.2:-36471888 None:intergenic
TGATGATATTTATGAAAAGT+TGG 0.457167 4.2:+36472161 MS.gene057639:CDS
GATATTTATGAAAAGTTGGC+AGG 0.457338 4.2:+36472165 MS.gene057639:CDS
TAAAACATCATAAGCTTCAA+AGG 0.465357 4.2:+36471917 MS.gene057639:CDS
AAAGGGCAACAACTTTGTTA+CGG 0.468035 4.2:+36471935 MS.gene057639:CDS
AGCTGCTCCTGCTGCTGGTG+AGG 0.474801 4.2:+36472416 MS.gene057639:CDS
ATTGAAGCTGCTCCTGCTGC+TGG 0.500424 4.2:+36472411 MS.gene057639:CDS
AACTAAAACAGGGTTGAAGA+AGG 0.504135 4.2:+36472296 MS.gene057639:CDS
AAGCAACAAAGAGGATTTGA+GGG 0.514889 4.2:+36472519 MS.gene057639:CDS
AAAACATCATAAGCTTCAAA+GGG 0.531611 4.2:+36471918 MS.gene057639:CDS
AATGGTGAGAATTTCGTATC+CGG 0.535348 4.2:+36472443 MS.gene057639:CDS
AAAGCAACAAAGAGGATTTG+AGG 0.536464 4.2:+36472518 MS.gene057639:CDS
ATCATCACGTTGTTTGCTCA+AGG 0.539246 4.2:+36472105 MS.gene057639:CDS
AAATATGATAGCACAAAAGA+AGG 0.540013 4.2:+36472225 MS.gene057639:CDS
TCGTTGCTCATCTCATCCTC+CGG 0.543243 4.2:-36472462 None:intergenic
TTTATTGCAAAGCAACAAAG+AGG 0.547495 4.2:+36472510 MS.gene057639:CDS
TGATTTCAAGGCTTTCTATG+AGG 0.550039 4.2:+36471986 MS.gene057639:CDS
ACTAAAACAGGGTTGAAGAA+GGG 0.598384 4.2:+36472297 MS.gene057639:CDS
GGAGTTTCGTCGTACTGTAG+AGG 0.600879 4.2:+36472485 MS.gene057639:CDS
GTGAGAGTGAGAACAGCGGA+AGG 0.613601 4.2:+36472382 MS.gene057639:CDS
GAACAAGAGAATTGATGATG+CGG 0.644326 4.2:+36472260 MS.gene057639:CDS
GGTGAGAATTTCGTATCCGG+AGG 0.672479 4.2:+36472446 MS.gene057639:CDS
AGATGTGAGAGTGAGAACAG+CGG 0.688021 4.2:+36472378 MS.gene057639:CDS
AGAAAATGAAAACTAAAACA+GGG 0.723999 4.2:+36472286 MS.gene057639:CDS
AGCAACAAAGAGGATTTGAG+GGG 0.729509 4.2:+36472520 MS.gene057639:CDS
CGATAACGAAGACAAAACAG+TGG 0.748693 4.2:-36472071 None:intergenic
GGAGGATGAGATGAGCAACG+AGG 0.778929 4.2:+36472464 MS.gene057639:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATGAAAACTAAAACA+GGG + chr4.2:36472286-36472305 MS.gene057639:CDS 20.0%
!! TGATGATATTTATGAAAAGT+TGG + chr4.2:36472161-36472180 MS.gene057639:CDS 20.0%
! AAAAAACATTCCAAAAGAAC+AGG + chr4.2:36472134-36472153 MS.gene057639:CDS 25.0%
! AAAACATCATAAGCTTCAAA+GGG + chr4.2:36471918-36471937 MS.gene057639:CDS 25.0%
! AAATATGATAGCACAAAAGA+AGG + chr4.2:36472225-36472244 MS.gene057639:CDS 25.0%
! GAGAAAATGAAAACTAAAAC+AGG + chr4.2:36472285-36472304 MS.gene057639:CDS 25.0%
! TAAAACATCATAAGCTTCAA+AGG + chr4.2:36471917-36471936 MS.gene057639:CDS 25.0%
!!! AAACTGAGATTTTGAAGAAA+CGG + chr4.2:36472340-36472359 MS.gene057639:CDS 25.0%
!!! TTTGAAGCTTATGATGTTTT+AGG - chr4.2:36471918-36471937 None:intergenic 25.0%
AGAAGGGTTATTGTTATAGA+AGG + chr4.2:36472313-36472332 MS.gene057639:CDS 30.0%
CAAACAACGTGATGATTATA+AGG - chr4.2:36472101-36472120 None:intergenic 30.0%
GATATTTATGAAAAGTTGGC+AGG + chr4.2:36472165-36472184 MS.gene057639:CDS 30.0%
TTTATTGCAAAGCAACAAAG+AGG + chr4.2:36472510-36472529 MS.gene057639:CDS 30.0%
AAACTCGAGCGAGTATTTAA+AGG + chr4.2:36472026-36472045 MS.gene057639:CDS 35.0%
AAAGCAACAAAGAGGATTTG+AGG + chr4.2:36472518-36472537 MS.gene057639:CDS 35.0%
AAAGGGCAACAACTTTGTTA+CGG + chr4.2:36471935-36471954 MS.gene057639:CDS 35.0%
AACTAAAACAGGGTTGAAGA+AGG + chr4.2:36472296-36472315 MS.gene057639:CDS 35.0%
AAGCAACAAAGAGGATTTGA+GGG + chr4.2:36472519-36472538 MS.gene057639:CDS 35.0%
AATGGTGAGAATTTCGTATC+CGG + chr4.2:36472443-36472462 MS.gene057639:CDS 35.0%
ACTAAAACAGGGTTGAAGAA+GGG + chr4.2:36472297-36472316 MS.gene057639:CDS 35.0%
TCATAGAAAGCCTTGAAATC+AGG - chr4.2:36471987-36472006 None:intergenic 35.0%
TGATTTCAAGGCTTTCTATG+AGG + chr4.2:36471986-36472005 MS.gene057639:CDS 35.0%
TTCTATGAGGTTACCAATTG+TGG + chr4.2:36471999-36472018 MS.gene057639:CDS 35.0%
! CACTGTTTTGTCTTCGTTAT+CGG + chr4.2:36472072-36472091 MS.gene057639:CDS 35.0%
! TCATCATGTTCCTGTTCTTT+TGG - chr4.2:36472147-36472166 None:intergenic 35.0%
!! GAACAAGAGAATTGATGATG+CGG + chr4.2:36472260-36472279 MS.gene057639:CDS 35.0%
!!! ATGATGTTTTAGGATTGCGT+TGG - chr4.2:36471908-36471927 None:intergenic 35.0%
!!! TTGTTTTGGTCCTGATTTCA+AGG + chr4.2:36471974-36471993 MS.gene057639:CDS 35.0%
AGCAACAAAGAGGATTTGAG+GGG + chr4.2:36472520-36472539 MS.gene057639:CDS 40.0%
ATCATCACGTTGTTTGCTCA+AGG + chr4.2:36472105-36472124 MS.gene057639:CDS 40.0%
CGATAACGAAGACAAAACAG+TGG - chr4.2:36472074-36472093 None:intergenic 40.0%
CTCGAGTTTCTTACCACAAT+TGG - chr4.2:36472015-36472034 None:intergenic 40.0%
!!! CGTTGAAGTGTGTGTTGTTT+TGG + chr4.2:36471960-36471979 MS.gene057639:CDS 40.0%
AGATGTGAGAGTGAGAACAG+CGG + chr4.2:36472378-36472397 MS.gene057639:CDS 45.0%
GGAGTTTCGTCGTACTGTAG+AGG + chr4.2:36472485-36472504 MS.gene057639:CDS 50.0%
GGTGAGAATTTCGTATCCGG+AGG + chr4.2:36472446-36472465 MS.gene057639:CDS 50.0%
TCGTTGCTCATCTCATCCTC+CGG - chr4.2:36472465-36472484 None:intergenic 50.0%
TGCTGCTGGTGAGGAGAAAA+TGG + chr4.2:36472425-36472444 MS.gene057639:CDS 50.0%
! ATTTTCTCCTCACCAGCAGC+AGG - chr4.2:36472426-36472445 None:intergenic 50.0%
!! TTGCGTTGGCTTTCTCTGCT+TGG - chr4.2:36471894-36471913 None:intergenic 50.0%
ATTGAAGCTGCTCCTGCTGC+TGG + chr4.2:36472411-36472430 MS.gene057639:CDS 55.0%
GGAGGATGAGATGAGCAACG+AGG + chr4.2:36472464-36472483 MS.gene057639:CDS 55.0%
GTGAGAGTGAGAACAGCGGA+AGG + chr4.2:36472382-36472401 MS.gene057639:CDS 55.0%
!! CGTTGGCTTTCTCTGCTTGG+AGG - chr4.2:36471891-36471910 None:intergenic 55.0%
AGCTGCTCCTGCTGCTGGTG+AGG + chr4.2:36472416-36472435 MS.gene057639:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 36471868 36472545 36471868 ID=MS.gene057639
chr4.2 mRNA 36471868 36472545 36471868 ID=MS.gene057639.t1;Parent=MS.gene057639
chr4.2 exon 36471868 36472545 36471868 ID=MS.gene057639.t1.exon1;Parent=MS.gene057639.t1
chr4.2 CDS 36471868 36472545 36471868 ID=cds.MS.gene057639.t1;Parent=MS.gene057639.t1
Gene Sequence

>MS.gene057639

ATGGAGTCGTTCAGCTTTAACCTCCAAGCAGAGAAAGCCAACGCAATCCTAAAACATCATAAGCTTCAAAGGGCAACAACTTTGTTACGGCTCGTTGAAGTGTGTGTTGTTTTGGTCCTGATTTCAAGGCTTTCTATGAGGTTACCAATTGTGGTAAGAAACTCGAGCGAGTATTTAAAGGATATTTCAGTCTTTATCAATAGCCACTGTTTTGTCTTCGTTATCGGAAACCTTATAATCATCACGTTGTTTGCTCAAGGTAATAGAAAAAACATTCCAAAAGAACAGGAACATGATGATATTTATGAAAAGTTGGCAGGCAAAAGTATAAAGCACGAAGAAAAAGAGAGAATAATAAAATATGATAGCACAAAAGAAGGTGCTAGCATAGAGAACAAGAGAATTGATGATGCGGAAGAGAAAATGAAAACTAAAACAGGGTTGAAGAAGGGTTATTGTTATAGAAGGTGTGAAACTGAGATTTTGAAGAAACGGCGTCGTGTGCTGCGAAGATGTGAGAGTGAGAACAGCGGAAGGAAAAAGATTGAAGCTGCTCCTGCTGCTGGTGAGGAGAAAATGGTGAGAATTTCGTATCCGGAGGATGAGATGAGCAACGAGGAGTTTCGTCGTACTGTAGAGGCTTTTATTGCAAAGCAACAAAGAGGATTTGAGGGGTAG

Protein sequence

>MS.gene057639.t1

MESFSFNLQAEKANAILKHHKLQRATTLLRLVEVCVVLVLISRLSMRLPIVVRNSSEYLKDISVFINSHCFVFVIGNLIIITLFAQGNRKNIPKEQEHDDIYEKLAGKSIKHEEKERIIKYDSTKEGASIENKRIDDAEEKMKTKTGLKKGYCYRRCETEILKKRRRVLRRCESENSGRKKIEAAPAAGEEKMVRISYPEDEMSNEEFRRTVEAFIAKQQRGFEG