AlfalfaGEDB | MS.gene24069

M. sativa cultivar XinJiangDaYe / MS.gene24069

KEGG: K11252
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0032991 GO:0032993 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00125.27

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24069.t1	XP_003590511.1	100	136	0	0	1	136	1	136	1.00E-44	189.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24069.t1	O22582	100.0	97	0	0	40	136	51	147	3.6e-46	185.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24069.t1	G7IBM3	100.0	136	0	0	1	136	1	136	7.4e-45	189.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051003	MS.gene24069	0.810472	1.13E-50	-1.69E-46
MS.gene053058	MS.gene24069	0.827915	1.20E-54	-1.69E-46
MS.gene056181	MS.gene24069	0.830137	3.48E-55	-1.69E-46
MS.gene058976	MS.gene24069	0.905862	2.74E-80	-1.69E-46
MS.gene059146	MS.gene24069	0.800342	1.51E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene24069	MS.gene025035	PPI
MS.gene24069	MS.gene99525	PPI
MS.gene24069	MS.gene75219	PPI
MS.gene24069	MS.gene004127	PPI
MS.gene24069	MS.gene025426	PPI
MS.gene24069	MS.gene004126	PPI
MS.gene24069	MS.gene64653	PPI
MS.gene24069	MS.gene066300	PPI
MS.gene24069	MS.gene049824	PPI
MS.gene24069	MS.gene041623	PPI
MS.gene066348	MS.gene24069	PPI
MS.gene24069	MS.gene24295	PPI
MS.gene24069	MS.gene26950	PPI
MS.gene24069	MS.gene63143	PPI
MS.gene24069	MS.gene051008	PPI
MS.gene24069	MS.gene81051	PPI
MS.gene24069	MS.gene65007	PPI
MS.gene24069	MS.gene63953	PPI
MS.gene24069	MS.gene025425	PPI
MS.gene24069	MS.gene84498	PPI
MS.gene24069	MS.gene27326	PPI
MS.gene24069	MS.gene066301	PPI
MS.gene24069	MS.gene049822	PPI
MS.gene24069	MS.gene035396	PPI
MS.gene24069	MS.gene08465	PPI
MS.gene24069	MS.gene65249	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24069.t1	MTR_1g068600	99.265	136	1	0	1	136	1	136	1.04e-93	266
MS.gene24069.t1	MTR_4g063240	85.034	147	11	2	1	136	1	147	1.77e-81	236
MS.gene24069.t1	MTR_4g063220	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene24069.t1	MTR_4g063990	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene24069.t1	MTR_4g063200	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene24069.t1	MTR_4g064020	85.811	148	9	2	1	136	1	148	4.40e-78	228
MS.gene24069.t1	MTR_1g090780	91.304	138	9	2	1	136	1	137	5.11e-78	227
MS.gene24069.t1	MTR_1g492910	91.304	138	9	2	1	136	1	137	5.89e-78	227
MS.gene24069.t1	MTR_4g071180	85.811	148	9	2	1	136	1	148	4.30e-77	225
MS.gene24069.t1	MTR_4g070240	85.811	148	9	2	1	136	1	148	4.30e-77	225
MS.gene24069.t1	MTR_4g088025	86.713	143	12	2	1	136	1	143	9.26e-76	221
MS.gene24069.t1	MTR_4g070245	84.459	148	11	2	1	136	1	148	1.54e-75	221
MS.gene24069.t1	MTR_4g063275	83.108	148	13	2	1	136	1	148	4.86e-75	220
MS.gene24069.t1	MTR_4g064983	84.722	144	10	2	1	132	1	144	2.53e-73	219
MS.gene24069.t1	MTR_7g099960	95.370	108	4	1	30	136	31	138	2.89e-64	192
MS.gene24069.t1	MTR_2g084480	62.921	89	33	0	47	135	136	224	9.85e-38	128
MS.gene24069.t1	MTR_8g069990	71.591	88	23	2	8	95	3	88	6.01e-35	117
MS.gene24069.t1	MTR_4g063270	90.196	51	5	0	74	124	1	51	5.77e-27	95.5
MS.gene24069.t1	MTR_7g114580	90.244	41	4	0	96	136	11	51	7.05e-20	77.4
MS.gene24069.t1	MTR_4g032777	39.744	78	47	0	43	120	33	110	1.43e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene24069.t1	AT3G45980	83.333	150	11	2	1	136	1	150	6.00e-77	225
MS.gene24069.t1	AT2G37470	88.406	138	13	2	1	135	1	138	7.78e-76	221
MS.gene24069.t1	AT1G07790	83.784	148	12	2	1	136	1	148	2.33e-75	221
MS.gene24069.t1	AT2G28720	83.444	151	10	2	1	136	1	151	2.26e-74	218
MS.gene24069.t1	AT3G53650	87.681	138	15	2	1	136	1	138	1.55e-73	216
MS.gene24069.t1	AT5G59910	87.407	135	14	1	5	136	16	150	6.19e-73	214
MS.gene24069.t1	AT3G46030	88.372	129	12	1	11	136	17	145	1.85e-71	211
MS.gene24069.t1	AT5G02570	83.571	140	11	2	1	136	1	132	1.41e-68	203
MS.gene24069.t1	AT3G09480	80.147	136	17	1	1	136	1	126	1.23e-65	195
MS.gene24069.t1	AT5G22880	81.119	143	17	2	4	136	3	145	2.62e-65	195
MS.gene24069.t1	AT1G08170	62.353	85	32	0	49	133	151	235	8.86e-38	128

Find 34 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGAAGATGTAAATCTTAT+AGG	0.133504	1.4:-54905850	None:intergenic
AATTCAAGAACTCGTAAATT+TGG	0.207431	1.4:-54906099	None:intergenic
TTCTTTCGAGATCTTCTTCT+CGG	0.293193	1.4:-54905784	None:intergenic
AGCGGGTTTCTTCTCTGCCT+TGG	0.302939	1.4:-54905715	None:intergenic
GCTGTGCGTTTGGTTCTTCC+TGG	0.321985	1.4:+54906032	MS.gene24069:CDS
TTTGAAGAAATACCTATATC+AGG	0.334896	1.4:-54905891	None:intergenic
GCGGGTTTCTTCTCTGCCTT+GGG	0.359903	1.4:-54905714	None:intergenic
CATCTTCAAGGTTCTCAAAC+AGG	0.361477	1.4:+54905863	MS.gene24069:CDS
GAGATCTTCTTCTCGGCTCT+TGG	0.362586	1.4:-54905777	None:intergenic
CTTCTCTGCCTTGGGAGCCA+TGG	0.363767	1.4:-54905706	None:intergenic
CTATAAGATTTACATCTTCA+AGG	0.370441	1.4:+54905851	MS.gene24069:CDS
GATTCAAACCGCTGTGCGTT+TGG	0.390239	1.4:+54906022	MS.gene24069:CDS
TTTGGTTCTTCCTGGTGAAT+TGG	0.398260	1.4:+54906040	MS.gene24069:CDS
AAACAGGTTCATCCTGATAT+AGG	0.421612	1.4:+54905879	MS.gene24069:CDS
AGGTATTTCTTCAAAAGCTA+TGG	0.429032	1.4:+54905899	MS.gene24069:CDS
CTTGTTGTAACGAGCCAATC+TGG	0.445538	1.4:-54905976	None:intergenic
TCCGCGGGTTTCTTCTCAGC+GGG	0.464857	1.4:-54905732	None:intergenic
TCTGCTGGTGATTTCTCCGC+GGG	0.470002	1.4:-54905747	None:intergenic
CGCTCAGGAAGCTTCCAGAT+TGG	0.475450	1.4:+54905962	MS.gene24069:CDS
TGCTGTTTCTGAAGGAACTA+AGG	0.493611	1.4:+54906070	MS.gene24069:CDS
GCATGTTTCGCCAATTCACC+AGG	0.516345	1.4:-54906050	None:intergenic
GGTATTTCTTCAAAAGCTAT+GGG	0.517184	1.4:+54905900	MS.gene24069:CDS
GCGAAACATGCTGTTTCTGA+AGG	0.530754	1.4:+54906062	MS.gene24069:CDS
ATTCAGATCCATGGCTCCCA+AGG	0.541897	1.4:+54905698	None:intergenic
GAAACCGACGATTACGTCAC+GGG	0.556515	1.4:+54905998	MS.gene24069:CDS
CTCTGCTGGTGATTTCTCCG+CGG	0.560516	1.4:-54905748	None:intergenic
GAGAAGAAGATCTCGAAAGA+AGG	0.564931	1.4:+54905786	MS.gene24069:CDS
TATCTTTGAGAAACTCGCTC+AGG	0.586744	1.4:+54905947	MS.gene24069:CDS
ATCTCCCGTGACGTAATCGT+CGG	0.590908	1.4:-54906002	None:intergenic
CTCCGCGGGTTTCTTCTCAG+CGG	0.628471	1.4:-54905733	None:intergenic
AAGAAGATCTCGAAAGAAGG+AGG	0.628797	1.4:+54905789	MS.gene24069:CDS
AGAAACCGACGATTACGTCA+CGG	0.672005	1.4:+54905997	MS.gene24069:CDS
ACCCGCTGAGAAGAAACCCG+CGG	0.680467	1.4:+54905731	MS.gene24069:CDS
AGGAAGAACCAAACGCACAG+CGG	0.757762	1.4:-54906030	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CTATAAGATTTACATCTTCA+AGG	+	chr1.4:54905851-54905870	MS.gene24069:CDS	25.0%
!	CTTGAAGATGTAAATCTTAT+AGG	-	chr1.4:54905853-54905872	None:intergenic	25.0%
!	TTTGAAGAAATACCTATATC+AGG	-	chr1.4:54905894-54905913	None:intergenic	25.0%
	AGGTATTTCTTCAAAAGCTA+TGG	+	chr1.4:54905899-54905918	MS.gene24069:CDS	30.0%
	GGTATTTCTTCAAAAGCTAT+GGG	+	chr1.4:54905900-54905919	MS.gene24069:CDS	30.0%
	AAACAGGTTCATCCTGATAT+AGG	+	chr1.4:54905879-54905898	MS.gene24069:CDS	35.0%
	TTCTTTCGAGATCTTCTTCT+CGG	-	chr1.4:54905787-54905806	None:intergenic	35.0%
	AAGAAGATCTCGAAAGAAGG+AGG	+	chr1.4:54905789-54905808	MS.gene24069:CDS	40.0%
	CATCTTCAAGGTTCTCAAAC+AGG	+	chr1.4:54905863-54905882	MS.gene24069:CDS	40.0%
	GAGAAGAAGATCTCGAAAGA+AGG	+	chr1.4:54905786-54905805	MS.gene24069:CDS	40.0%
!	TATCTTTGAGAAACTCGCTC+AGG	+	chr1.4:54905947-54905966	MS.gene24069:CDS	40.0%
!!	TGCTGTTTCTGAAGGAACTA+AGG	+	chr1.4:54906070-54906089	MS.gene24069:CDS	40.0%
!!	TTTGGTTCTTCCTGGTGAAT+TGG	+	chr1.4:54906040-54906059	MS.gene24069:CDS	40.0%
	AGAAACCGACGATTACGTCA+CGG	+	chr1.4:54905997-54906016	MS.gene24069:CDS	45.0%
!	CTTGTTGTAACGAGCCAATC+TGG	-	chr1.4:54905979-54905998	None:intergenic	45.0%
!!	GCGAAACATGCTGTTTCTGA+AGG	+	chr1.4:54906062-54906081	MS.gene24069:CDS	45.0%
	AGGAAGAACCAAACGCACAG+CGG	-	chr1.4:54906033-54906052	None:intergenic	50.0%
	ATCTCCCGTGACGTAATCGT+CGG	-	chr1.4:54906005-54906024	None:intergenic	50.0%
	GAAACCGACGATTACGTCAC+GGG	+	chr1.4:54905998-54906017	MS.gene24069:CDS	50.0%
	GAGATCTTCTTCTCGGCTCT+TGG	-	chr1.4:54905780-54905799	None:intergenic	50.0%
	GATTCAAACCGCTGTGCGTT+TGG	+	chr1.4:54906022-54906041	MS.gene24069:CDS	50.0%
	GCATGTTTCGCCAATTCACC+AGG	-	chr1.4:54906053-54906072	None:intergenic	50.0%
!!	GCTCTTGGCTTTTTCTCTGC+TGG	-	chr1.4:54905765-54905784	None:intergenic	50.0%
	CGCTCAGGAAGCTTCCAGAT+TGG	+	chr1.4:54905962-54905981	MS.gene24069:CDS	55.0%
	CTCTGCTGGTGATTTCTCCG+CGG	-	chr1.4:54905751-54905770	None:intergenic	55.0%
	GCGGGTTTCTTCTCTGCCTT+GGG	-	chr1.4:54905717-54905736	None:intergenic	55.0%
	TCTGCTGGTGATTTCTCCGC+GGG	-	chr1.4:54905750-54905769	None:intergenic	55.0%
!	AGCGGGTTTCTTCTCTGCCT+TGG	-	chr1.4:54905718-54905737	None:intergenic	55.0%
!!	GCTGTGCGTTTGGTTCTTCC+TGG	+	chr1.4:54906032-54906051	MS.gene24069:CDS	55.0%
	ACCCGCTGAGAAGAAACCCG+CGG	+	chr1.4:54905731-54905750	MS.gene24069:CDS	60.0%
	CTCCGCGGGTTTCTTCTCAG+CGG	-	chr1.4:54905736-54905755	None:intergenic	60.0%
	TCCGCGGGTTTCTTCTCAGC+GGG	-	chr1.4:54905735-54905754	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	54905708	54906118	54905708	ID=MS.gene24069
chr1.4	mRNA	54905708	54906118	54905708	ID=MS.gene24069.t1;Parent=MS.gene24069
chr1.4	exon	54905708	54906118	54905708	ID=MS.gene24069.t1.exon1;Parent=MS.gene24069.t1
chr1.4	CDS	54905708	54906118	54905708	ID=cds.MS.gene24069.t1;Parent=MS.gene24069.t1

Gene Sequence

>MS.gene24069

ATGGCTCCCAAGGCAGAGAAGAAACCCGCTGAGAAGAAACCCGCGGAGAAATCACCAGCAGAGAAAAAGCCAAGAGCCGAGAAGAAGATCTCGAAAGAAGGAGGCGATAAGAAGAAGAAGCGCGTGAAGAAGAGCGTTGAAACCTATAAGATTTACATCTTCAAGGTTCTCAAACAGGTTCATCCTGATATAGGTATTTCTTCAAAAGCTATGGGAATTATGAACAGTTTCATCAATGATATCTTTGAGAAACTCGCTCAGGAAGCTTCCAGATTGGCTCGTTACAACAAGAAACCGACGATTACGTCACGGGAGATTCAAACCGCTGTGCGTTTGGTTCTTCCTGGTGAATTGGCGAAACATGCTGTTTCTGAAGGAACTAAGGCTGTTACCAAATTTACGAGTTCTTGA

Protein sequence

>MS.gene24069.t1

MAPKAEKKPAEKKPAEKSPAEKKPRAEKKISKEGGDKKKKRVKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS