Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25343.t1 | ABN08740.1 | 80.3 | 61 | 12 | 0 | 25 | 85 | 127 | 187 | 7.60E-06 | 59.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25343.t1 | G7L3K3 | 80.3 | 61 | 12 | 0 | 25 | 85 | 127 | 187 | 5.5e-06 | 59.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene25343 | 0.804573 | 2.02E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene25343 | 0.809869 | 1.53E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene25343 | 0.819778 | 9.71E-53 | -1.69E-46 |
| MS.gene052926 | MS.gene25343 | 0.819027 | 1.44E-52 | -1.69E-46 |
| MS.gene05308 | MS.gene25343 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene25343 | 0.809727 | 1.64E-50 | -1.69E-46 |
| MS.gene054657 | MS.gene25343 | 0.81191 | 5.53E-51 | -1.69E-46 |
| MS.gene056498 | MS.gene25343 | 0.800274 | 1.56E-48 | -1.69E-46 |
| MS.gene056776 | MS.gene25343 | 0.804317 | 2.29E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene25343 | 0.813395 | 2.62E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene25343 | 0.803832 | 2.89E-49 | -1.69E-46 |
| MS.gene057414 | MS.gene25343 | 0.801514 | 8.69E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene25343 | 0.829237 | 5.76E-55 | -1.69E-46 |
| MS.gene058979 | MS.gene25343 | 0.800396 | 1.47E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene25343 | 0.810287 | 1.24E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene25343 | 0.803435 | 3.49E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene25343 | -0.802414 | 5.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGCCTAGTCCACATTTC+TGG | 0.233776 | 8.2:-38933615 | MS.gene25343:CDS |
| CTGGAACAACGTTTCGAAAC+TGG | 0.311123 | 8.2:-38933596 | None:intergenic |
| GAGATTGAAGGTGGAGAAGA+AGG | 0.384382 | 8.2:-38933793 | MS.gene25343:CDS |
| GTGGACTAAGGAACAAGAAT+TGG | 0.455229 | 8.2:-38933662 | MS.gene25343:CDS |
| AAGGTGGAGAAGAAGGGAAA+AGG | 0.458473 | 8.2:-38933786 | MS.gene25343:CDS |
| GCACGATGGTTGTGAAATAG+TGG | 0.487049 | 8.2:-38933761 | MS.gene25343:CDS |
| GGGAAGAAGGAAGAGATTGA+AGG | 0.500449 | 8.2:-38933805 | MS.gene25343:CDS |
| ATACAAAGTTTCAACTCAGA+AGG | 0.508154 | 8.2:-38933829 | MS.gene25343:CDS |
| AGGAAGAGAAAGCGCGGTGG+TGG | 0.525493 | 8.2:-38933700 | MS.gene25343:CDS |
| TACAAAGTTTCAACTCAGAA+GGG | 0.527223 | 8.2:-38933828 | MS.gene25343:CDS |
| GAAACGTTGTTCCAGAAATG+TGG | 0.531004 | 8.2:+38933604 | None:intergenic |
| GTGAAATAGTGGAAGAGTTG+AGG | 0.532067 | 8.2:-38933750 | MS.gene25343:CDS |
| AAGAAGGAAGAGATTGAAGG+TGG | 0.534159 | 8.2:-38933802 | MS.gene25343:CDS |
| AATAGTGGAAGAGTTGAGGA+AGG | 0.535399 | 8.2:-38933746 | MS.gene25343:CDS |
| AGATTGAAGGTGGAGAAGAA+GGG | 0.536092 | 8.2:-38933792 | MS.gene25343:CDS |
| ACAAAGTTTCAACTCAGAAG+GGG | 0.540390 | 8.2:-38933827 | MS.gene25343:CDS |
| TTGTTCCAGAAATGTGGACT+AGG | 0.553064 | 8.2:+38933610 | None:intergenic |
| ACAAGAATTGGCTCTGCGAA+CGG | 0.574620 | 8.2:-38933650 | MS.gene25343:CDS |
| AAGTTGAATGTGGTGTGAAA+AGG | 0.575392 | 8.2:-38933720 | MS.gene25343:CDS |
| AGTTGCACAAGGGTGGACTA+AGG | 0.587210 | 8.2:-38933674 | MS.gene25343:CDS |
| CAAAGTTTCAACTCAGAAGG+GGG | 0.603686 | 8.2:-38933826 | MS.gene25343:CDS |
| GGTGGTGGTGAAGTTGCACA+AGG | 0.634091 | 8.2:-38933685 | MS.gene25343:CDS |
| GTGGTGAAGTTGCACAAGGG+TGG | 0.656463 | 8.2:-38933681 | MS.gene25343:CDS |
| AGGAAGGAAGAAGTTGAATG+TGG | 0.671131 | 8.2:-38933730 | MS.gene25343:CDS |
| AAAGTTTCAACTCAGAAGGG+GGG | 0.695511 | 8.2:-38933825 | MS.gene25343:CDS |
| GTGGTGGTGAAGTTGCACAA+GGG | 0.698386 | 8.2:-38933684 | MS.gene25343:CDS |
| GTGAAAAGGAAGAGAAAGCG+CGG | 0.698757 | 8.2:-38933706 | MS.gene25343:CDS |
| GAAGGGAAAAGGAAGCACGA+TGG | 0.741513 | 8.2:-38933775 | MS.gene25343:CDS |
| AAAAGGAAGAGAAAGCGCGG+TGG | 0.749477 | 8.2:-38933703 | MS.gene25343:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATACAAAGTTTCAACTCAGA+AGG | - | chr8.2:38933599-38933618 | MS.gene25343:CDS | 30.0% | |
| TACAAAGTTTCAACTCAGAA+GGG | - | chr8.2:38933600-38933619 | MS.gene25343:CDS | 30.0% | |
| AAGTTGAATGTGGTGTGAAA+AGG | - | chr8.2:38933708-38933727 | MS.gene25343:CDS | 35.0% | |
| ACAAAGTTTCAACTCAGAAG+GGG | - | chr8.2:38933601-38933620 | MS.gene25343:CDS | 35.0% | |
| AAAGTTTCAACTCAGAAGGG+GGG | - | chr8.2:38933603-38933622 | MS.gene25343:CDS | 40.0% | |
| AAGAAGGAAGAGATTGAAGG+TGG | - | chr8.2:38933626-38933645 | MS.gene25343:CDS | 40.0% | |
| AGATTGAAGGTGGAGAAGAA+GGG | - | chr8.2:38933636-38933655 | MS.gene25343:CDS | 40.0% | |
| AGGAAGGAAGAAGTTGAATG+TGG | - | chr8.2:38933698-38933717 | MS.gene25343:CDS | 40.0% | |
| CAAAGTTTCAACTCAGAAGG+GGG | - | chr8.2:38933602-38933621 | MS.gene25343:CDS | 40.0% | |
| GAAACGTTGTTCCAGAAATG+TGG | + | chr8.2:38933827-38933846 | None:intergenic | 40.0% | |
| TTGTTCCAGAAATGTGGACT+AGG | + | chr8.2:38933821-38933840 | None:intergenic | 40.0% | |
| ! | AATAGTGGAAGAGTTGAGGA+AGG | - | chr8.2:38933682-38933701 | MS.gene25343:CDS | 40.0% |
| ! | GTGAAATAGTGGAAGAGTTG+AGG | - | chr8.2:38933678-38933697 | MS.gene25343:CDS | 40.0% |
| ! | GTGGACTAAGGAACAAGAAT+TGG | - | chr8.2:38933766-38933785 | MS.gene25343:CDS | 40.0% |
| AAGGTGGAGAAGAAGGGAAA+AGG | - | chr8.2:38933642-38933661 | MS.gene25343:CDS | 45.0% | |
| CAAAGCCTAGTCCACATTTC+TGG | - | chr8.2:38933813-38933832 | MS.gene25343:CDS | 45.0% | |
| GAGATTGAAGGTGGAGAAGA+AGG | - | chr8.2:38933635-38933654 | MS.gene25343:CDS | 45.0% | |
| GCACGATGGTTGTGAAATAG+TGG | - | chr8.2:38933667-38933686 | MS.gene25343:CDS | 45.0% | |
| GGGAAGAAGGAAGAGATTGA+AGG | - | chr8.2:38933623-38933642 | MS.gene25343:CDS | 45.0% | |
| GTGAAAAGGAAGAGAAAGCG+CGG | - | chr8.2:38933722-38933741 | MS.gene25343:CDS | 45.0% | |
| !! | ACAAGAATTGGCTCTGCGAA+CGG | - | chr8.2:38933778-38933797 | MS.gene25343:CDS | 45.0% |
| ! | AAAAGGAAGAGAAAGCGCGG+TGG | - | chr8.2:38933725-38933744 | MS.gene25343:CDS | 50.0% |
| ! | GAAGGGAAAAGGAAGCACGA+TGG | - | chr8.2:38933653-38933672 | MS.gene25343:CDS | 50.0% |
| ! | GTGGTGGTGAAGTTGCACAA+GGG | - | chr8.2:38933744-38933763 | MS.gene25343:CDS | 50.0% |
| !! | AGTTGCACAAGGGTGGACTA+AGG | - | chr8.2:38933754-38933773 | MS.gene25343:CDS | 50.0% |
| CAACTCAGAAGGGGGGAAGA+AGG | - | chr8.2:38933610-38933629 | MS.gene25343:CDS | 55.0% | |
| GTGGTGAAGTTGCACAAGGG+TGG | - | chr8.2:38933747-38933766 | MS.gene25343:CDS | 55.0% | |
| ! | GGTGGTGGTGAAGTTGCACA+AGG | - | chr8.2:38933743-38933762 | MS.gene25343:CDS | 55.0% |
| ! | AGGAAGAGAAAGCGCGGTGG+TGG | - | chr8.2:38933728-38933747 | MS.gene25343:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 38933597 | 38933853 | 38933597 | ID=MS.gene25343 |
| chr8.2 | mRNA | 38933597 | 38933853 | 38933597 | ID=MS.gene25343.t1;Parent=MS.gene25343 |
| chr8.2 | exon | 38933597 | 38933853 | 38933597 | ID=MS.gene25343.t1.exon1;Parent=MS.gene25343.t1 |
| chr8.2 | CDS | 38933597 | 38933853 | 38933597 | ID=cds.MS.gene25343.t1;Parent=MS.gene25343.t1 |
| Gene Sequence |
| Protein sequence |
