AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25898

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25898.t1 MTR_3g078740 94.324 599 32 1 1 599 1 597 0.0 1179
MS.gene25898.t1 MTR_3g078700 76.311 591 87 3 1 590 1 539 0.0 933
MS.gene25898.t1 MTR_3g078730 73.833 600 103 4 1 599 1 547 0.0 884
MS.gene25898.t1 MTR_8g091390 47.049 576 269 10 23 594 31 574 0.0 539
MS.gene25898.t1 MTR_7g058690 32.368 587 327 18 7 567 14 556 9.12e-71 239
MS.gene25898.t1 MTR_5g081810 33.880 549 288 22 41 569 14 507 1.21e-70 237
MS.gene25898.t1 MTR_5g069680 32.935 586 326 19 1 570 3 537 8.26e-70 236
MS.gene25898.t1 MTR_4g015120 32.358 581 331 22 1 569 5 535 6.45e-69 233
MS.gene25898.t1 MTR_7g060460 31.687 587 329 18 7 565 13 555 2.01e-68 233
MS.gene25898.t1 MTR_7g458880 31.472 591 329 19 4 565 11 554 1.11e-67 231
MS.gene25898.t1 MTR_7g062250 33.277 592 307 24 7 565 16 552 3.05e-67 229
MS.gene25898.t1 MTR_5g083360 30.229 612 351 20 1 584 1 564 5.75e-67 228
MS.gene25898.t1 MTR_5g020600 31.017 590 310 21 13 570 21 545 3.07e-65 224
MS.gene25898.t1 MTR_2g008330 30.717 586 336 17 7 569 19 557 5.10e-65 223
MS.gene25898.t1 MTR_4g064530 32.218 568 313 24 22 565 40 559 2.78e-64 221
MS.gene25898.t1 MTR_5g073210 30.565 602 332 23 9 579 4 550 2.99e-64 221
MS.gene25898.t1 MTR_3g462760 31.922 567 319 19 20 570 20 535 2.37e-63 218
MS.gene25898.t1 MTR_5g020620 30.508 590 319 21 8 569 13 539 4.54e-63 218
MS.gene25898.t1 MTR_8g027375 30.159 567 342 19 16 569 24 549 2.74e-62 216
MS.gene25898.t1 MTR_7g062310 31.966 585 321 21 26 580 47 584 6.37e-62 215
MS.gene25898.t1 MTR_3g101635 29.766 598 335 21 1 569 3 544 6.85e-62 214
MS.gene25898.t1 MTR_3g101640 30.288 591 348 23 1 569 1 549 7.56e-59 206
MS.gene25898.t1 MTR_4g064530 31.495 562 313 24 22 559 40 553 2.94e-57 202
MS.gene25898.t1 MTR_7g065980 28.995 607 327 26 7 569 3 549 8.19e-56 198
MS.gene25898.t1 MTR_4g132320 30.087 575 325 19 26 590 31 538 1.67e-55 197
MS.gene25898.t1 MTR_7g065970 27.529 603 348 22 1 569 1 548 7.92e-53 190
MS.gene25898.t1 MTR_8g479390 27.226 584 334 20 25 590 27 537 1.17e-52 189
MS.gene25898.t1 MTR_4g049390 27.367 581 327 17 26 587 27 531 3.02e-52 188
MS.gene25898.t1 MTR_3g071890 31.449 566 315 26 3 547 13 526 6.12e-52 187
MS.gene25898.t1 MTR_5g018270 28.862 589 319 22 20 587 22 531 6.91e-52 187
MS.gene25898.t1 MTR_1g090707 28.336 547 285 17 45 564 43 509 3.57e-50 183
MS.gene25898.t1 MTR_4g019225 29.152 566 334 20 28 569 28 550 5.08e-50 182
MS.gene25898.t1 MTR_3g115050 26.878 599 350 19 8 590 11 537 5.81e-50 182
MS.gene25898.t1 MTR_4g083190 25.993 604 362 22 7 592 7 543 2.23e-49 180
MS.gene25898.t1 MTR_3g083390 27.417 569 313 21 49 592 48 541 4.03e-49 179
MS.gene25898.t1 MTR_3g083440 27.434 565 318 21 49 592 48 541 7.27e-49 179
MS.gene25898.t1 MTR_3g083400 27.611 565 317 21 49 592 48 541 1.90e-48 177
MS.gene25898.t1 MTR_4g048350 26.817 578 333 17 26 587 27 530 1.92e-48 177
MS.gene25898.t1 MTR_3g083430 27.241 569 314 21 49 592 48 541 2.18e-48 177
MS.gene25898.t1 MTR_8g479380 26.534 603 337 23 12 587 9 532 3.32e-48 177
MS.gene25898.t1 MTR_3g083450 27.417 569 313 22 49 592 49 542 5.28e-48 176
MS.gene25898.t1 MTR_3g083410 27.128 564 321 20 49 592 12 505 8.54e-48 175
MS.gene25898.t1 MTR_4g101650 27.855 578 343 20 28 587 29 550 1.45e-47 176
MS.gene25898.t1 MTR_1g026410 26.325 585 337 18 26 591 29 538 2.98e-47 174
MS.gene25898.t1 MTR_4g075690 25.828 604 353 18 1 587 8 533 3.19e-47 174
MS.gene25898.t1 MTR_3g008580 26.399 572 339 17 38 587 41 552 1.38e-46 173
MS.gene25898.t1 MTR_7g051440 26.644 578 357 20 28 592 31 554 1.10e-45 171
MS.gene25898.t1 MTR_2g088080 25.734 579 339 15 26 587 30 534 2.94e-45 168
MS.gene25898.t1 MTR_3g079660 75.258 97 23 1 502 597 5 101 7.83e-44 152
MS.gene25898.t1 MTR_4g083210 26.585 568 315 21 49 592 48 537 6.89e-41 156
MS.gene25898.t1 MTR_4g075690 25.912 521 290 18 1 506 8 447 2.31e-35 139
MS.gene25898.t1 MTR_2g088080 24.190 525 308 14 80 587 2 453 3.18e-34 135
MS.gene25898.t1 MTR_2g009230 86.885 61 8 0 131 191 1 61 2.67e-31 117
MS.gene25898.t1 MTR_5g081790 31.629 313 160 14 257 549 142 420 3.49e-21 97.1
MS.gene25898.t1 MTR_5g081790 34.667 150 86 5 6 148 7 151 4.65e-17 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25898.t1 AT5G21105 62.957 575 186 3 23 595 35 584 0.0 763
MS.gene25898.t1 AT5G21100 62.872 571 178 6 30 595 28 569 0.0 732
MS.gene25898.t1 AT5G21105 62.571 529 171 3 23 549 35 538 0.0 694
MS.gene25898.t1 AT4G39830 48.097 578 263 9 23 591 33 582 0.0 558
MS.gene25898.t1 AT5G21105 61.029 408 132 3 190 595 11 393 0.0 517
MS.gene25898.t1 AT4G39830 47.758 513 231 9 88 591 3 487 3.13e-164 478
MS.gene25898.t1 AT4G39830 47.750 511 230 9 90 591 3 485 6.12e-164 477
MS.gene25898.t1 AT4G39830 45.809 513 228 10 88 591 3 474 7.10e-152 446
MS.gene25898.t1 AT5G58910 29.766 598 338 17 1 569 1 545 1.61e-65 224
MS.gene25898.t1 AT1G18140 30.241 582 337 19 20 572 23 564 8.49e-65 223
MS.gene25898.t1 AT5G03260 29.798 594 324 23 7 570 6 536 9.56e-65 222
MS.gene25898.t1 AT5G58910 29.933 598 336 18 1 569 1 544 2.08e-64 221
MS.gene25898.t1 AT2G29130 32.993 588 325 23 1 565 6 547 2.61e-64 221
MS.gene25898.t1 AT2G30210 29.706 579 332 19 17 569 19 548 6.20e-62 215
MS.gene25898.t1 AT5G48100 30.691 593 319 24 13 569 5 541 1.04e-61 214
MS.gene25898.t1 AT5G05390 29.443 574 324 18 23 569 24 543 1.25e-61 214
MS.gene25898.t1 AT2G46570 30.035 566 332 18 22 569 28 547 1.37e-61 214
MS.gene25898.t1 AT2G40370 29.223 592 346 20 8 569 10 558 2.04e-60 211
MS.gene25898.t1 AT5G07130 29.545 616 347 21 3 586 9 569 6.17e-60 209
MS.gene25898.t1 AT5G09360 29.630 594 335 20 1 569 12 547 7.42e-60 209
MS.gene25898.t1 AT5G60020 30.640 594 338 23 1 565 3 551 7.82e-60 209
MS.gene25898.t1 AT5G01040 29.762 588 318 21 16 569 18 544 1.58e-59 209
MS.gene25898.t1 AT3G09220 29.843 573 316 20 28 569 28 545 1.67e-59 208
MS.gene25898.t1 AT5G60020 31.227 554 310 20 41 565 8 519 3.64e-58 204
MS.gene25898.t1 AT5G01190 30.864 567 314 19 26 569 25 536 1.84e-57 202
MS.gene25898.t1 AT5G58910 29.422 554 306 17 41 569 8 501 4.26e-57 201
MS.gene25898.t1 AT2G38080 30.580 569 337 21 13 569 14 536 5.53e-57 201
MS.gene25898.t1 AT5G01050 29.776 581 329 21 16 569 18 546 1.89e-56 200
MS.gene25898.t1 AT5G01190 31.079 547 299 19 46 569 31 522 1.23e-55 197
MS.gene25898.t1 AT4G12420 27.036 614 365 23 1 592 1 553 7.56e-55 196
MS.gene25898.t1 AT4G12420 27.036 614 365 23 1 592 1 553 7.56e-55 196
MS.gene25898.t1 AT4G22010 28.179 582 322 19 26 587 26 531 8.63e-53 189
MS.gene25898.t1 AT5G60020 30.104 578 330 23 1 549 3 535 2.29e-52 188
MS.gene25898.t1 AT1G55570 29.038 582 316 22 30 587 33 541 7.92e-52 187
MS.gene25898.t1 AT1G75790 27.346 618 339 23 4 587 1 542 8.37e-52 187
MS.gene25898.t1 AT5G66920 26.460 548 317 16 28 564 39 511 1.11e-50 184
MS.gene25898.t1 AT4G28090 27.136 597 353 19 9 590 9 538 4.38e-50 182
MS.gene25898.t1 AT1G21860 26.396 591 349 17 16 591 16 535 5.37e-50 182
MS.gene25898.t1 AT1G21850 26.520 592 339 17 16 587 16 531 1.01e-49 181
MS.gene25898.t1 AT5G51480 26.700 603 353 20 16 592 17 556 1.30e-49 182
MS.gene25898.t1 AT4G25240 26.230 610 369 18 4 592 7 556 1.40e-49 181
MS.gene25898.t1 AT4G25240 26.230 610 369 18 4 592 7 556 1.40e-49 181
MS.gene25898.t1 AT3G13390 28.816 583 316 24 30 587 32 540 7.20e-49 179
MS.gene25898.t1 AT1G76160 25.624 601 360 17 6 590 5 534 3.08e-48 177
MS.gene25898.t1 AT5G48450 25.285 613 357 19 9 592 8 548 1.45e-47 176
MS.gene25898.t1 AT5G48450 25.285 613 357 19 9 592 8 548 2.97e-47 176
MS.gene25898.t1 AT2G46570 29.167 504 294 17 84 569 4 462 3.76e-47 172
MS.gene25898.t1 AT4G37160 24.877 611 359 18 3 591 7 539 1.23e-46 172
MS.gene25898.t1 AT2G23630 26.817 578 329 19 28 587 37 538 1.80e-45 169
MS.gene25898.t1 AT2G23630 26.817 578 329 19 28 587 32 533 2.18e-45 169
MS.gene25898.t1 AT5G07130 28.653 527 298 19 92 586 4 484 1.05e-43 163
MS.gene25898.t1 AT5G07130 28.653 527 298 19 92 586 4 484 1.05e-43 163
MS.gene25898.t1 AT1G41830 23.411 598 365 17 7 587 11 532 6.86e-42 159
MS.gene25898.t1 AT4G38420 24.831 592 357 17 19 590 20 543 7.04e-40 153
MS.gene25898.t1 AT3G09220 28.000 500 277 18 100 569 2 448 1.98e-39 150
MS.gene25898.t1 AT3G13400 25.690 580 340 18 2 564 5 510 5.50e-39 151
MS.gene25898.t1 AT3G13400 25.690 580 340 18 2 564 5 510 5.50e-39 151
MS.gene25898.t1 AT1G55560 25.683 549 329 17 28 564 28 509 1.25e-38 150
MS.gene25898.t1 AT4G37160 24.952 521 296 17 3 506 7 449 4.30e-34 135
MS.gene25898.t1 AT4G38420 24.528 530 317 15 19 533 20 481 1.86e-31 128
MS.gene25898.t1 AT4G38420 24.453 503 297 15 19 506 20 454 1.86e-28 119

Find 136 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGACACGTGCACCTTTA+AGG 0.136465 3.2:+69524104 MS.gene25898:CDS
CTGAGGGTGTTCACAAAATT+AGG 0.188685 3.2:+69524251 MS.gene25898:CDS
CCGTTACCGGTAGTTGTATT+TGG 0.192418 3.2:-69523914 None:intergenic
GTGATTGGTGGTGGTGAGTT+TGG 0.204305 3.2:-69523605 None:intergenic
AAAACAAATCATTTCCAAAA+TGG 0.220018 3.2:+69523667 MS.gene25898:CDS
CATATGGTTGGACTGCATTA+AGG 0.257525 3.2:+69524146 MS.gene25898:CDS
AATGGCTCTTTATGCCTCTT+TGG 0.282560 3.2:-69521051 None:intergenic
CTTACTCTGTAAGTCTTGTT+TGG 0.288383 3.2:-69522919 None:intergenic
GTAGTTGTATTTGGATTAAC+TGG 0.289880 3.2:-69523905 None:intergenic
ACAACCACGGTGTTCCTTAA+AGG 0.291405 3.2:-69524118 None:intergenic
CACTACCATTCCCATGTAAC+TGG 0.296143 3.2:-69523991 None:intergenic
TGGCTGTGGAGTTCCCATTT+TGG 0.299259 3.2:-69523681 None:intergenic
TGATTGGTGGTGGTGAGTTT+GGG 0.300160 3.2:-69523604 None:intergenic
GGCTTCACTTGAAGAATATA+TGG 0.305282 3.2:-69522898 None:intergenic
TGGATTGCAATTGGTGTTAG+AGG 0.327840 3.2:+69523440 MS.gene25898:CDS
AGGCAGATAATCCTGGTGTT+TGG 0.327845 3.2:+69524173 MS.gene25898:CDS
TTGTATGGTGTATATGGTTT+GGG 0.330107 3.2:+69520155 MS.gene25898:CDS
TAATCGGTAGTTACACGAAA+TGG 0.330187 3.2:+69523750 MS.gene25898:CDS
CACCACCAATCACTCCATTA+TGG 0.348399 3.2:+69523615 MS.gene25898:CDS
TGTGAATGCTTTGCTATGTT+TGG 0.349636 3.2:-69523645 None:intergenic
CTCAATGAAGGGTGTCGTTT+AGG 0.351859 3.2:-69524321 None:intergenic
ATCACCTTTGAAATTACATT+GGG 0.353634 3.2:-69522863 None:intergenic
GGGATGTTTGAGCTATCATT+TGG 0.355283 3.2:+69520175 MS.gene25898:CDS
ATTTCGTGTAACTACCGATT+AGG 0.356859 3.2:-69523748 None:intergenic
ATCTGCTCCTCAGTGATCTA+TGG 0.363548 3.2:+69521094 MS.gene25898:CDS
CATTGAACAACATTTGCATA+TGG 0.364666 3.2:+69524210 MS.gene25898:CDS
TCTGACTCAATCGGTGTTAG+AGG 0.366295 3.2:+69523515 MS.gene25898:CDS
TTAAATATGTCATAATCCTT+TGG 0.368436 3.2:-69523878 None:intergenic
GGAATCAATGGTCAGTTTCC+TGG 0.370438 3.2:+69520274 MS.gene25898:CDS
GGCAGATAATCCTGGTGTTT+GGG 0.374826 3.2:+69524174 MS.gene25898:CDS
ATCCTGCGGTGTGAGAATTT+TGG 0.380966 3.2:-69523704 None:intergenic
TTTGTATGGTGTATATGGTT+TGG 0.381937 3.2:+69520154 MS.gene25898:CDS
GTTAAACCTTGTGGCGTGTT+TGG 0.401829 3.2:-69523545 None:intergenic
AAACATGTAAACACCGTTAC+CGG 0.405766 3.2:-69523927 None:intergenic
TGTTGGTAACATTAATGATA+AGG 0.406661 3.2:-69520327 None:intergenic
GTTGGTAACATTAATGATAA+GGG 0.411709 3.2:-69520326 None:intergenic
GTTTGCAGCGTGGAACTCCT+TGG 0.412682 3.2:+69520508 MS.gene25898:intron
TCCTTCTGTAGAAAGATTGT+TGG 0.412761 3.2:-69520344 None:intergenic
TTTGTAGTTAAGGATTGTTA+TGG 0.412986 3.2:-69523486 None:intergenic
AAACAAATCATTTCCAAAAT+GGG 0.418944 3.2:+69523668 MS.gene25898:CDS
TGTTTGGTATCATTCCATAA+TGG 0.420105 3.2:-69523629 None:intergenic
TATTTCTATCATGGACACTA+TGG 0.424783 3.2:+69520982 MS.gene25898:CDS
CTCAACACACAAAACCTAAT+CGG 0.428454 3.2:+69523734 MS.gene25898:CDS
TATTTGATTGATCCTAAGTA+AGG 0.430267 3.2:-69523809 None:intergenic
TTGTATTGAAAAGTTTCTCC+TGG 0.430789 3.2:-69520570 None:intergenic
TTTGGAAATGTCTCTGGTGG+TGG 0.431712 3.2:-69523860 None:intergenic
CAACTTCAGCTCTGATAGTT+GGG 0.433763 3.2:-69520297 None:intergenic
CTTAATGCAGTCCAACCATA+TGG 0.434255 3.2:-69524145 None:intergenic
AAAGAGCCATTTCATTATGA+TGG 0.435519 3.2:+69521063 MS.gene25898:CDS
AACTCACCATCATAATGAAA+TGG 0.437277 3.2:-69521069 None:intergenic
TTTGCAGCGTGGAACTCCTT+GGG 0.437651 3.2:+69520509 MS.gene25898:intron
GGTTCACCGGTCCATCTCAT+TGG 0.443786 3.2:-69521159 None:intergenic
CAAAGAGCAGCAGGGTTGTA+TGG 0.444015 3.2:+69521009 MS.gene25898:CDS
TTTCAGACACTGCTCATCAA+TGG 0.444816 3.2:+69522787 MS.gene25898:intron
ATTGAACAACATTTGCATAT+GGG 0.447437 3.2:+69524211 MS.gene25898:CDS
CAAACAAGACTTACAGAGTA+AGG 0.451124 3.2:+69522920 MS.gene25898:CDS
ACCGTGGTTGTATTTCCATA+TGG 0.456218 3.2:+69524130 MS.gene25898:CDS
TAATCCTTTGGAAATGTCTC+TGG 0.456862 3.2:-69523866 None:intergenic
CCAACTTCAGCTCTGATAGT+TGG 0.458597 3.2:-69520298 None:intergenic
TCATTCCATAATGGAGTGAT+TGG 0.463471 3.2:-69523620 None:intergenic
GATCCTAAGTAAGGTGTTGT+TGG 0.466961 3.2:-69523800 None:intergenic
TTGGACTGCATTAAGGTTCA+AGG 0.470182 3.2:+69524153 MS.gene25898:CDS
CATCACCTTTGAAATTACAT+TGG 0.470295 3.2:-69522864 None:intergenic
AATCGGTAGTTACACGAAAT+GGG 0.479350 3.2:+69523751 MS.gene25898:CDS
CATGGTACATATTTCTATCA+TGG 0.481061 3.2:+69520973 MS.gene25898:CDS
CAGAATGCAAACCAGTTACA+TGG 0.482253 3.2:+69523980 MS.gene25898:CDS
CGTGGAACTCCTTGGGCTGA+TGG 0.482269 3.2:+69520516 MS.gene25898:CDS
ACCACATGCTCTATGCAATC+TGG 0.482605 3.2:-69520247 None:intergenic
TATGGTATGCAAAGAGCAGC+AGG 0.488270 3.2:+69521000 MS.gene25898:CDS
TTGCCAACAACACCTTACTT+AGG 0.489614 3.2:+69523797 MS.gene25898:CDS
AAAACAATCTCTGACTCAAT+CGG 0.491178 3.2:+69523506 MS.gene25898:CDS
CTTTGAAATTACATTGGGGT+AGG 0.493306 3.2:-69522858 None:intergenic
TTAAGGATTGTTATGGCTTG+TGG 0.496023 3.2:-69523479 None:intergenic
TCTTCACAAATTTAGCTGCC+AGG 0.508771 3.2:-69522831 None:intergenic
TGGTTGTATTTCCATATGGT+TGG 0.513701 3.2:+69524134 MS.gene25898:CDS
TCAATGTCATCGACTGCGAA+TGG 0.514346 3.2:-69523353 None:intergenic
AATGTATACCTGTGGTTCAC+CGG 0.516965 3.2:-69521172 None:intergenic
CAATGTCATCGACTGCGAAT+GGG 0.518080 3.2:-69523352 None:intergenic
ACAACATTTGCATATGGGAA+TGG 0.521997 3.2:+69524216 MS.gene25898:CDS
TTAAGGTTTGTTAAACCTTG+TGG 0.529917 3.2:-69523554 None:intergenic
CCAAATACAACTACCGGTAA+CGG 0.532128 3.2:+69523914 MS.gene25898:CDS
TCTCTTCAGCTCCAATGAGA+TGG 0.532184 3.2:+69521148 MS.gene25898:CDS
CAGAAGGAACTGTTATTCAC+TGG 0.532868 3.2:+69520359 MS.gene25898:CDS
CAATGAAACGCCCAAACACC+AGG 0.533302 3.2:-69524184 None:intergenic
CTTCACAAATTTAGCTGCCA+GGG 0.534636 3.2:-69522830 None:intergenic
GTTAATCCAAATACAACTAC+CGG 0.535206 3.2:+69523908 MS.gene25898:CDS
ACCAACAATCTTTCTACAGA+AGG 0.538143 3.2:+69520343 MS.gene25898:CDS
TTGCATAGAGCATGTGGTGA+TGG 0.538505 3.2:+69520252 MS.gene25898:CDS
TTTGAAATTACATTGGGGTA+GGG 0.539787 3.2:-69522857 None:intergenic
TCTCAGTGTGCTATAAATCC+AGG 0.548391 3.2:+69520552 MS.gene25898:CDS
TCAGCTCCAATGAGATGGAC+CGG 0.549768 3.2:+69521153 MS.gene25898:CDS
CAACATTTGCATATGGGAAT+GGG 0.550546 3.2:+69524217 MS.gene25898:CDS
TGAGTAACTAGTCTCAATGA+AGG 0.552455 3.2:-69524333 None:intergenic
GGTGTCGTTTAGGAACATCT+TGG 0.554463 3.2:-69524311 None:intergenic
TCAGCTCTGATAGTTGGGCC+AGG 0.554876 3.2:-69520292 None:intergenic
AGGTTCAAGGCAGATAATCC+TGG 0.555260 3.2:+69524166 MS.gene25898:CDS
ATTCATCCATGGCATTTGCA+TGG 0.556495 3.2:+69524016 MS.gene25898:CDS
GGAACTGTTATTCACTGGCA+TGG 0.556886 3.2:+69520364 MS.gene25898:CDS
GCAAACCAGTTACATGGGAA+TGG 0.558379 3.2:+69523986 MS.gene25898:CDS
AGGACAATTCAATTGTTCCC+TGG 0.563840 3.2:+69522813 MS.gene25898:CDS
GAAATTACATTGGGGTAGGG+TGG 0.566061 3.2:-69522854 None:intergenic
GAGATGGACCGGTGAACCAC+AGG 0.571005 3.2:+69521164 MS.gene25898:CDS
CACCAAAATTCTCACACCGC+AGG 0.571410 3.2:+69523702 MS.gene25898:CDS
CCAACTATCAGAGCTGAAGT+TGG 0.571850 3.2:+69520298 MS.gene25898:CDS
CTTGTTGTAGTAGAAGCAGA+TGG 0.582483 3.2:+69523317 MS.gene25898:CDS
CACAAGCTCACGTGAACAAG+AGG 0.584256 3.2:+69521119 MS.gene25898:CDS
GATGACATTGATATCTACTC+AGG 0.587375 3.2:+69523365 MS.gene25898:CDS
TCACTGGCATGGAATCAGAC+AGG 0.589797 3.2:+69520375 MS.gene25898:CDS
CTACCCCAATGTAATTTCAA+AGG 0.592553 3.2:+69522859 MS.gene25898:CDS
CATGTGGTGATGGGAATCAA+TGG 0.593479 3.2:+69520262 MS.gene25898:CDS
TGCATAGAGCATGTGGTGAT+GGG 0.594219 3.2:+69520253 MS.gene25898:CDS
TTCCATAATGGAGTGATTGG+TGG 0.606846 3.2:-69523617 None:intergenic
TCATGTCCATGCAAATGCCA+TGG 0.613062 3.2:-69524022 None:intergenic
AGCTCACGTGAACAAGAGGT+TGG 0.616753 3.2:+69521123 MS.gene25898:CDS
ACCAGATTGCATAGAGCATG+TGG 0.617569 3.2:+69520246 MS.gene25898:CDS
AGAAAACCAAACACGCCACA+AGG 0.625328 3.2:+69523539 MS.gene25898:CDS
GTCATTAGCTTCTCTCAACA+TGG 0.626821 3.2:+69522957 MS.gene25898:CDS
ATCTCATTGGAGCTGAAGAG+AGG 0.627315 3.2:-69521146 None:intergenic
CATAATGGAGTGATTGGTGG+TGG 0.630467 3.2:-69523614 None:intergenic
ATGGTAGTGAAATTCATCCA+TGG 0.631220 3.2:+69524005 MS.gene25898:CDS
ACCACCAGAGACATTTCCAA+AGG 0.631679 3.2:+69523862 MS.gene25898:CDS
TAATAGTAGATTTACCAAAG+AGG 0.633241 3.2:+69521037 MS.gene25898:CDS
GAGTAACTAGTCTCAATGAA+GGG 0.635616 3.2:-69524332 None:intergenic
TCCTTTGGAAATGTCTCTGG+TGG 0.636748 3.2:-69523863 None:intergenic
ATACAAATTCGAAGTTGACA+AGG 0.645769 3.2:+69520587 MS.gene25898:CDS
AGAATGCAAACCAGTTACAT+GGG 0.646441 3.2:+69523981 MS.gene25898:CDS
GCAGCAGTTCCATCAGCCCA+AGG 0.647903 3.2:-69520525 None:intergenic
CCTACAGAAGCTCTTACTTG+TGG 0.668732 3.2:+69524280 MS.gene25898:CDS
ACACTGCTCATCAATGGACG+AGG 0.682587 3.2:+69522793 MS.gene25898:CDS
ATGGTATGCAAAGAGCAGCA+GGG 0.689403 3.2:+69521001 MS.gene25898:CDS
ACCATATGGAAATACAACCA+CGG 0.699908 3.2:-69524131 None:intergenic
CCACAAGTAAGAGCTTCTGT+AGG 0.711257 3.2:-69524280 None:intergenic
TCACCTTTGAAATTACATTG+GGG 0.718792 3.2:-69522862 None:intergenic
TGTTGAGGAGAAGAATCCTG+CGG 0.770585 3.2:-69523718 None:intergenic
TGCACCTTTAAGGAACACCG+TGG 0.772778 3.2:+69524114 MS.gene25898:CDS
TTGTGTGCCATAGATCACTG+AGG 0.786407 3.2:-69521101 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+GGG + chr3.2:69521229-69521248 MS.gene25898:intron 0.0%
!! TATATATATATATATATATA+TGG + chr3.2:69521230-69521249 MS.gene25898:intron 0.0%
!! AAAAAAATAAAAAATACACA+CGG - chr3.2:69520760-69520779 None:intergenic 10.0%
!! AGAGTTATTAAAAAAAAAAA+AGG + chr3.2:69523134-69523153 MS.gene25898:intron 10.0%
!! TAGATAATTAAAGATATTAA+CGG + chr3.2:69522106-69522125 MS.gene25898:intron 10.0%
!! TGTGTATATATATATATATA+GGG - chr3.2:69521539-69521558 None:intergenic 10.0%
!! TTAATTTAATATTGCTTTAA+CGG + chr3.2:69520921-69520940 MS.gene25898:intron 10.0%
!!! TTTGTTTCAATTATTTTATT+GGG + chr3.2:69522458-69522477 MS.gene25898:intron 10.0%
!! AAAAAATGTGACATAAAAAT+AGG - chr3.2:69522399-69522418 None:intergenic 15.0%
!! GTGTATATATATATATATAG+GGG - chr3.2:69521538-69521557 None:intergenic 15.0%
!! GTGTGTATATATATATATAT+AGG - chr3.2:69521540-69521559 None:intergenic 15.0%
!! TAATTTAATATTGCTTTAAC+GGG + chr3.2:69520922-69520941 MS.gene25898:intron 15.0%
!! TATACATTAGTTAAAAAGTA+TGG - chr3.2:69522002-69522021 None:intergenic 15.0%
!! TGTATATATATATATATAGG+GGG - chr3.2:69521537-69521556 None:intergenic 15.0%
!! TTCAGATTAATAAGAAATTA+TGG + chr3.2:69520817-69520836 MS.gene25898:intron 15.0%
!!! ATTTATTTCAGTTGTTTTAT+TGG - chr3.2:69521720-69521739 None:intergenic 15.0%
!!! GTTTGTTTCAATTATTTTAT+TGG + chr3.2:69522457-69522476 MS.gene25898:intron 15.0%
!!! TATTATTTGTTTTTATCATG+TGG + chr3.2:69522234-69522253 MS.gene25898:intron 15.0%
!!! TGTAAATATTTTTTCTCTTT+GGG - chr3.2:69521817-69521836 None:intergenic 15.0%
!!! TTGTAAATATTTTTTCTCTT+TGG - chr3.2:69521818-69521837 None:intergenic 15.0%
!! AAAAACGAATGGAATAATTT+AGG + chr3.2:69523097-69523116 MS.gene25898:intron 20.0%
!! AAAAATAGGAATAGGAAATT+GGG - chr3.2:69522385-69522404 None:intergenic 20.0%
!! AAAACAAATCATTTCCAAAA+TGG + chr3.2:69523667-69523686 MS.gene25898:CDS 20.0%
!! AAACAAATCATTTCCAAAAT+GGG + chr3.2:69523668-69523687 MS.gene25898:CDS 20.0%
!! AAAGTTTAATAGAGACAAAA+TGG + chr3.2:69521436-69521455 MS.gene25898:intron 20.0%
!! CAAATGCATAATATGATAAA+AGG + chr3.2:69522490-69522509 MS.gene25898:intron 20.0%
!! TAAAAATAGGAATAGGAAAT+TGG - chr3.2:69522386-69522405 None:intergenic 20.0%
!! TACAAATAAGAAAAGAAGAT+TGG + chr3.2:69521764-69521783 MS.gene25898:intron 20.0%
!! TATTAAATTAAACACACAGT+TGG - chr3.2:69520912-69520931 None:intergenic 20.0%
!! TTAAATATGTCATAATCCTT+TGG - chr3.2:69523881-69523900 None:intergenic 20.0%
!!! AAAAAAAAAAAAGGTGTGAA+AGG + chr3.2:69523143-69523162 MS.gene25898:intron 20.0%
!!! AAAAAAAAAAAGGTGTGAAA+GGG + chr3.2:69523144-69523163 MS.gene25898:intron 20.0%
!!! AATCTTCTTTTCTTATTTGT+AGG - chr3.2:69521765-69521784 None:intergenic 20.0%
!!! TAAATTATTCCATTCGTTTT+TGG - chr3.2:69523098-69523117 None:intergenic 20.0%
!!! TAATTTTGATCATTCTAATC+TGG - chr3.2:69521885-69521904 None:intergenic 20.0%
!!! TATTTTTTTTTCGACAAAGA+TGG - chr3.2:69523020-69523039 None:intergenic 20.0%
!!! TGGAATAAAAATTAGTTTGT+GGG - chr3.2:69522611-69522630 None:intergenic 20.0%
!!! TGTGTGAATATTTTTTCTTT+GGG + chr3.2:69522333-69522352 MS.gene25898:intron 20.0%
!!! TTAACTATGCGTTTTTATAA+TGG - chr3.2:69522716-69522735 None:intergenic 20.0%
!!! TTTATGTTGCCAATTTTTAT+TGG + chr3.2:69522643-69522662 MS.gene25898:intron 20.0%
! AACACAAATAAATGTGGATA+TGG + chr3.2:69522515-69522534 MS.gene25898:intron 25.0%
! AAGGAAAACACAAATAAATG+TGG + chr3.2:69522509-69522528 MS.gene25898:intron 25.0%
! AATGCTAATTACAATTAGAC+TGG - chr3.2:69520449-69520468 None:intergenic 25.0%
! AGATCAAAAACTTACTGAAA+TGG - chr3.2:69522983-69523002 None:intergenic 25.0%
! AGTACAGTTTGTATGAAAAA+TGG - chr3.2:69520879-69520898 None:intergenic 25.0%
! ATAGTAATATGTAAATCTCC+AGG - chr3.2:69523063-69523082 None:intergenic 25.0%
! ATAGTGCATATTGCAAAAAT+TGG - chr3.2:69521961-69521980 None:intergenic 25.0%
! ATTCAACCACCAATAAAAAT+TGG - chr3.2:69522655-69522674 None:intergenic 25.0%
! ATTGAACAACATTTGCATAT+GGG + chr3.2:69524211-69524230 MS.gene25898:CDS 25.0%
! GAATGGAATAATTTAGGTAA+CGG + chr3.2:69523103-69523122 MS.gene25898:intron 25.0%
! GTACAGTTTGTATGAAAAAT+GGG - chr3.2:69520878-69520897 None:intergenic 25.0%
! TAATAGTAGATTTACCAAAG+AGG + chr3.2:69521037-69521056 MS.gene25898:CDS 25.0%
! TATTTGATTGATCCTAAGTA+AGG - chr3.2:69523812-69523831 None:intergenic 25.0%
! TGTGACATAAAAATAGGAAT+AGG - chr3.2:69522393-69522412 None:intergenic 25.0%
! TTAAGAAAGACTTGACTAAT+AGG + chr3.2:69523174-69523193 MS.gene25898:intron 25.0%
! TTTAATTCACCAAAAACGAA+TGG + chr3.2:69523086-69523105 MS.gene25898:intron 25.0%
! TTTGTAGTTAAGGATTGTTA+TGG - chr3.2:69523489-69523508 None:intergenic 25.0%
!! GATTTTCACGAAAAATATGA+AGG - chr3.2:69521671-69521690 None:intergenic 25.0%
!! GTGGAATAAAAATTAGTTTG+TGG - chr3.2:69522612-69522631 None:intergenic 25.0%
!!! AGTCTTTCTTTTTTGTTAAG+TGG + chr3.2:69522295-69522314 MS.gene25898:intron 25.0%
!!! ATATTTTTTCTTTGGGTCAA+TGG + chr3.2:69522340-69522359 MS.gene25898:intron 25.0%
!!! ATCACCTTTGAAATTACATT+GGG - chr3.2:69522866-69522885 None:intergenic 25.0%
!!! CTGTGTGAATATTTTTTCTT+TGG + chr3.2:69522332-69522351 MS.gene25898:intron 25.0%
!!! GACATGTATTTTAAAACTGA+GGG - chr3.2:69521939-69521958 None:intergenic 25.0%
!!! GTTGGTAACATTAATGATAA+GGG - chr3.2:69520329-69520348 None:intergenic 25.0%
!!! TATTTTTTCTTTGGGTCAAT+GGG + chr3.2:69522341-69522360 MS.gene25898:intron 25.0%
!!! TGGAATAAATCTTTTTTTCC+TGG - chr3.2:69521865-69521884 None:intergenic 25.0%
!!! TGTTGGTAACATTAATGATA+AGG - chr3.2:69520330-69520349 None:intergenic 25.0%
!!! TGTTTTAGTAAAGTACAAGT+AGG + chr3.2:69521459-69521478 MS.gene25898:intron 25.0%
!!! TGTTTTGGTAAATAACTAGT+AGG - chr3.2:69521349-69521368 None:intergenic 25.0%
AAAAACTACTGGATTGCAAT+TGG + chr3.2:69523431-69523450 MS.gene25898:CDS 30.0%
AAAACAATCTCTGACTCAAT+CGG + chr3.2:69523506-69523525 MS.gene25898:CDS 30.0%
AAAAGTTGAATGTATACCTG+TGG - chr3.2:69521183-69521202 None:intergenic 30.0%
AAAATGCTAGATATCACGAA+TGG - chr3.2:69521693-69521712 None:intergenic 30.0%
AAAGAGCCATTTCATTATGA+TGG + chr3.2:69521063-69521082 MS.gene25898:CDS 30.0%
AACATTAATATGTTAGGGTG+TGG - chr3.2:69520851-69520870 None:intergenic 30.0%
AACTCACCATCATAATGAAA+TGG - chr3.2:69521072-69521091 None:intergenic 30.0%
ACATTAATATGTTAGGGTGT+GGG - chr3.2:69520850-69520869 None:intergenic 30.0%
ATACAAATTCGAAGTTGACA+AGG + chr3.2:69520587-69520606 MS.gene25898:CDS 30.0%
ATATAATAATGTTTGCAGCG+TGG + chr3.2:69520498-69520517 MS.gene25898:intron 30.0%
CATTGAACAACATTTGCATA+TGG + chr3.2:69524210-69524229 MS.gene25898:CDS 30.0%
CCATATACACCATACAAAAA+TGG - chr3.2:69520152-69520171 None:intergenic 30.0%
GACAAAAAACTAATTTCCAC+TGG - chr3.2:69521617-69521636 None:intergenic 30.0%
GCTGGAACATTAATATGTTA+GGG - chr3.2:69520856-69520875 None:intergenic 30.0%
GTAGTTGTATTTGGATTAAC+TGG - chr3.2:69523908-69523927 None:intergenic 30.0%
GTTAATCCAAATACAACTAC+CGG + chr3.2:69523908-69523927 MS.gene25898:CDS 30.0%
TAATTACCATAGTGTGTGAT+AGG - chr3.2:69522763-69522782 None:intergenic 30.0%
TGGGTGTTTGATTATACTTA+CGG + chr3.2:69520945-69520964 MS.gene25898:intron 30.0%
TGTTTGGTATCATTCCATAA+TGG - chr3.2:69523632-69523651 None:intergenic 30.0%
TTGTATGGTGTATATGGTTT+GGG + chr3.2:69520155-69520174 MS.gene25898:CDS 30.0%
TTGTATTGAAAAGTTTCTCC+TGG - chr3.2:69520573-69520592 None:intergenic 30.0%
TTTAATATTGCTTTAACGGG+TGG + chr3.2:69520925-69520944 MS.gene25898:intron 30.0%
TTTGTATGGTGTATATGGTT+TGG + chr3.2:69520154-69520173 MS.gene25898:CDS 30.0%
! ATAGAAAAACACGAGTGTTT+TGG - chr3.2:69521364-69521383 None:intergenic 30.0%
! ATGTTGCCAATTTTTATTGG+TGG + chr3.2:69522646-69522665 MS.gene25898:intron 30.0%
! ATTTGCACCTTAGTGTTTTT+AGG - chr3.2:69521314-69521333 None:intergenic 30.0%
! ATTTGCATGGACATGATTTT+TGG + chr3.2:69524029-69524048 MS.gene25898:CDS 30.0%
! CATGGTACATATTTCTATCA+TGG + chr3.2:69520973-69520992 MS.gene25898:CDS 30.0%
! TATTTCTATCATGGACACTA+TGG + chr3.2:69520982-69521001 MS.gene25898:CDS 30.0%
! TTAATATTGCTTTAACGGGT+GGG + chr3.2:69520926-69520945 MS.gene25898:intron 30.0%
! TTTGAAATTACATTGGGGTA+GGG - chr3.2:69522860-69522879 None:intergenic 30.0%
! TTTGCATGGACATGATTTTT+GGG + chr3.2:69524030-69524049 MS.gene25898:CDS 30.0%
! TTTTAACCTATCACACACTA+TGG + chr3.2:69522754-69522773 MS.gene25898:intron 30.0%
! TTTTCTTTCTCTTGTTCTAG+TGG + chr3.2:69520402-69520421 MS.gene25898:intron 30.0%
!! AACATAAACTAACTTGCTAG+TGG - chr3.2:69522631-69522650 None:intergenic 30.0%
!! CAGAGATTGTTTTGTAGTTA+AGG - chr3.2:69523499-69523518 None:intergenic 30.0%
!! CATCACCTTTGAAATTACAT+TGG - chr3.2:69522867-69522886 None:intergenic 30.0%
!! CCATTTTTGTATGGTGTATA+TGG + chr3.2:69520149-69520168 MS.gene25898:CDS 30.0%
!! CTTGGACATGTATTTTGAAA+CGG + chr3.2:69522164-69522183 MS.gene25898:intron 30.0%
!! TCACCTTTGAAATTACATTG+GGG - chr3.2:69522865-69522884 None:intergenic 30.0%
!! TTAAGGTTTGTTAAACCTTG+TGG - chr3.2:69523557-69523576 None:intergenic 30.0%
!!! ATTTTTTCTTTGGGTCAATG+GGG + chr3.2:69522342-69522361 MS.gene25898:intron 30.0%
!!! GGACATGTATTTTAAAACTG+AGG - chr3.2:69521940-69521959 None:intergenic 30.0%
!!! TGTTCTAGTGGTTTTAGTTA+TGG + chr3.2:69520414-69520433 MS.gene25898:intron 30.0%
!!! TGTTTTAATGAGTACTGAGT+AGG - chr3.2:69523396-69523415 None:intergenic 30.0%
!!! TTTTTTCTCTTTGGGTGAAT+AGG - chr3.2:69521809-69521828 None:intergenic 30.0%
AAACATGTAAACACCGTTAC+CGG - chr3.2:69523930-69523949 None:intergenic 35.0%
AATCGGTAGTTACACGAAAT+GGG + chr3.2:69523751-69523770 MS.gene25898:CDS 35.0%
ACAACATTTGCATATGGGAA+TGG + chr3.2:69524216-69524235 MS.gene25898:CDS 35.0%
ACCAACAATCTTTCTACAGA+AGG + chr3.2:69520343-69520362 MS.gene25898:CDS 35.0%
AGAATGCAAACCAGTTACAT+GGG + chr3.2:69523981-69524000 MS.gene25898:CDS 35.0%
AGACTCATATTACACATACC+AGG + chr3.2:69521844-69521863 MS.gene25898:intron 35.0%
AGTTTGTATGAAAAATGGGC+TGG - chr3.2:69520874-69520893 None:intergenic 35.0%
ATCACTTATTTGTGCACTAG+TGG + chr3.2:69520708-69520727 MS.gene25898:intron 35.0%
CAAACAAGACTTACAGAGTA+AGG + chr3.2:69522920-69522939 MS.gene25898:CDS 35.0%
CAACATTTGCATATGGGAAT+GGG + chr3.2:69524217-69524236 MS.gene25898:CDS 35.0%
CTACCCCAATGTAATTTCAA+AGG + chr3.2:69522859-69522878 MS.gene25898:CDS 35.0%
CTCAACACACAAAACCTAAT+CGG + chr3.2:69523734-69523753 MS.gene25898:CDS 35.0%
CTGCAGCATAGACAAATTAT+GGG - chr3.2:69523288-69523307 None:intergenic 35.0%
GATGACATTGATATCTACTC+AGG + chr3.2:69523365-69523384 MS.gene25898:CDS 35.0%
GGCTGGAACATTAATATGTT+AGG - chr3.2:69520857-69520876 None:intergenic 35.0%
GGCTTCACTTGAAGAATATA+TGG - chr3.2:69522901-69522920 None:intergenic 35.0%
GTACATTGACATATGTGAAG+TGG + chr3.2:69522137-69522156 MS.gene25898:intron 35.0%
GTTCATTCAATTTACACCAG+TGG + chr3.2:69521598-69521617 MS.gene25898:intron 35.0%
TAATCGGTAGTTACACGAAA+TGG + chr3.2:69523750-69523769 MS.gene25898:CDS 35.0%
TAATGAATTTCCTCCTCTAG+TGG + chr3.2:69520614-69520633 MS.gene25898:intron 35.0%
TCCTTCTGTAGAAAGATTGT+TGG - chr3.2:69520347-69520366 None:intergenic 35.0%
TCTGCAGCATAGACAAATTA+TGG - chr3.2:69523289-69523308 None:intergenic 35.0%
TGCAGCATAGACAAATTATG+GGG - chr3.2:69523287-69523306 None:intergenic 35.0%
TGGTTGTATTTCCATATGGT+TGG + chr3.2:69524134-69524153 MS.gene25898:CDS 35.0%
TTAAGGATTGTTATGGCTTG+TGG - chr3.2:69523482-69523501 None:intergenic 35.0%
TTGAAGAATGATCTGATCCA+TGG - chr3.2:69523255-69523274 None:intergenic 35.0%
TTTCATCGTTAATTTCGCCA+TGG + chr3.2:69523235-69523254 MS.gene25898:intron 35.0%
! ACTTGCACCTTAGTGTTTTT+AGG + chr3.2:69521494-69521513 MS.gene25898:intron 35.0%
! ATGGTAGTGAAATTCATCCA+TGG + chr3.2:69524005-69524024 MS.gene25898:CDS 35.0%
! ATTTCGTGTAACTACCGATT+AGG - chr3.2:69523751-69523770 None:intergenic 35.0%
! GCAAAACGCATCTTTTCATT+TGG - chr3.2:69521639-69521658 None:intergenic 35.0%
! GGGAGTATGTTTATATCATG+TGG - chr3.2:69521919-69521938 None:intergenic 35.0%
! TAATCCTTTGGAAATGTCTC+TGG - chr3.2:69523869-69523888 None:intergenic 35.0%
! TCATTCCATAATGGAGTGAT+TGG - chr3.2:69523623-69523642 None:intergenic 35.0%
! TGTGAATGCTTTGCTATGTT+TGG - chr3.2:69523648-69523667 None:intergenic 35.0%
! TTGAAACTTTTCGCATCACT+TGG - chr3.2:69524085-69524104 None:intergenic 35.0%
!! CTTACTCTGTAAGTCTTGTT+TGG - chr3.2:69522922-69522941 None:intergenic 35.0%
!! CTTTGAAATTACATTGGGGT+AGG - chr3.2:69522861-69522880 None:intergenic 35.0%
!!! AATGGGTGTTATTTTTGCTG+AGG + chr3.2:69524234-69524253 MS.gene25898:CDS 35.0%
!!! AGACAGAGCTTGTTTTGTTA+AGG - chr3.2:69523574-69523593 None:intergenic 35.0%
!!! ATGGGTGTTATTTTTGCTGA+GGG + chr3.2:69524235-69524254 MS.gene25898:CDS 35.0%
!!! CAATCCAGTAGTTTTTTGAG+GGG - chr3.2:69523427-69523446 None:intergenic 35.0%
!!! GATTTTTGGGTTTTGGGATA+TGG + chr3.2:69524043-69524062 MS.gene25898:CDS 35.0%
!!! GCAATCCAGTAGTTTTTTGA+GGG - chr3.2:69523428-69523447 None:intergenic 35.0%
!!! GGACATGATTTTTGGGTTTT+GGG + chr3.2:69524037-69524056 MS.gene25898:CDS 35.0%
!!! TGCAATCCAGTAGTTTTTTG+AGG - chr3.2:69523429-69523448 None:intergenic 35.0%
!!! TGGACATGATTTTTGGGTTT+TGG + chr3.2:69524036-69524055 MS.gene25898:CDS 35.0%
!!! TGTTGAGAGCCATTTTTGTA+TGG + chr3.2:69520140-69520159 MS.gene25898:CDS 35.0%
AAGACCCCTCAAAAAACTAC+TGG + chr3.2:69523420-69523439 MS.gene25898:CDS 40.0%
AATGGCTCTTTATGCCTCTT+TGG - chr3.2:69521054-69521073 None:intergenic 40.0%
AATGTATACCTGTGGTTCAC+CGG - chr3.2:69521175-69521194 None:intergenic 40.0%
AGGACAATTCAATTGTTCCC+TGG + chr3.2:69522813-69522832 MS.gene25898:CDS 40.0%
ATTATACTTACGGTGCAGCA+TGG + chr3.2:69520955-69520974 MS.gene25898:intron 40.0%
ATTCATCCATGGCATTTGCA+TGG + chr3.2:69524016-69524035 MS.gene25898:CDS 40.0%
ATTTCATTGTTCGCACTTCC+TGG + chr3.2:69523042-69523061 MS.gene25898:intron 40.0%
ATTTGACACGTGCACCTTTA+AGG + chr3.2:69524104-69524123 MS.gene25898:CDS 40.0%
CAGAAGGAACTGTTATTCAC+TGG + chr3.2:69520359-69520378 MS.gene25898:CDS 40.0%
CAGAATGCAAACCAGTTACA+TGG + chr3.2:69523980-69523999 MS.gene25898:CDS 40.0%
CATATGGTTGGACTGCATTA+AGG + chr3.2:69524146-69524165 MS.gene25898:CDS 40.0%
CATATGTGAAGTGGTTGACT+TGG + chr3.2:69522146-69522165 MS.gene25898:intron 40.0%
CCAAATACAACTACCGGTAA+CGG + chr3.2:69523914-69523933 MS.gene25898:CDS 40.0%
CTTAATGCAGTCCAACCATA+TGG - chr3.2:69524148-69524167 None:intergenic 40.0%
CTTCACAAATTTAGCTGCCA+GGG - chr3.2:69522833-69522852 None:intergenic 40.0%
GGGATGTTTGAGCTATCATT+TGG + chr3.2:69520175-69520194 MS.gene25898:CDS 40.0%
TCTCAGTGTGCTATAAATCC+AGG + chr3.2:69520552-69520571 MS.gene25898:CDS 40.0%
TCTTCACAAATTTAGCTGCC+AGG - chr3.2:69522834-69522853 None:intergenic 40.0%
TTGCCAACAACACCTTACTT+AGG + chr3.2:69523797-69523816 MS.gene25898:CDS 40.0%
TTGGACTGCATTAAGGTTCA+AGG + chr3.2:69524153-69524172 MS.gene25898:CDS 40.0%
TTTCAGACACTGCTCATCAA+TGG + chr3.2:69522787-69522806 MS.gene25898:intron 40.0%
! CAACTTCAGCTCTGATAGTT+GGG - chr3.2:69520300-69520319 None:intergenic 40.0%
! CTTGTTGTAGTAGAAGCAGA+TGG + chr3.2:69523317-69523336 MS.gene25898:CDS 40.0%
! GTCATTAGCTTCTCTCAACA+TGG + chr3.2:69522957-69522976 MS.gene25898:CDS 40.0%
! TGAGAGTGTGATCTTCTACT+TGG - chr3.2:69523200-69523219 None:intergenic 40.0%
! TTCCATAATGGAGTGATTGG+TGG - chr3.2:69523620-69523639 None:intergenic 40.0%
!! ATATGAAGAAGTGCCACTAG+AGG - chr3.2:69520630-69520649 None:intergenic 40.0%
!! CGATTAGGTTTTGTGTGTTG+AGG - chr3.2:69523736-69523755 None:intergenic 40.0%
!! CTGAGGGTGTTCACAAAATT+AGG + chr3.2:69524251-69524270 MS.gene25898:CDS 40.0%
!! GATCCTAAGTAAGGTGTTGT+TGG - chr3.2:69523803-69523822 None:intergenic 40.0%
!! GTTATGGCTTGTGGTGTTTT+TGG - chr3.2:69523473-69523492 None:intergenic 40.0%
!! TGGATTGCAATTGGTGTTAG+AGG + chr3.2:69523440-69523459 MS.gene25898:CDS 40.0%
!!! AGTAGTTTTTTGAGGGGTCT+TGG - chr3.2:69523421-69523440 None:intergenic 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG + chr3.2:69521498-69521517 MS.gene25898:intron 40.0%
!!! GCACCTTAGTGTTTTTAGGT+GGG - chr3.2:69521517-69521498 None:intergenic 40.0%
!!! GGACATGTATTTTGAAACGG+AGG + chr3.2:69522167-69522186 MS.gene25898:intron 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG + chr3.2:69521497-69521516 MS.gene25898:intron 40.0%
!!! TGCACCTTAGTGTTTTTAGG+TGG - chr3.2:69521516-69521497 None:intergenic 40.0%
ACCACATGCTCTATGCAATC+TGG - chr3.2:69520250-69520269 None:intergenic 45.0%
ACCACCAGAGACATTTCCAA+AGG + chr3.2:69523862-69523881 MS.gene25898:CDS 45.0%
ACCAGATTGCATAGAGCATG+TGG + chr3.2:69520246-69520265 MS.gene25898:CDS 45.0%
AGAAAACCAAACACGCCACA+AGG + chr3.2:69523539-69523558 MS.gene25898:CDS 45.0%
AGGCAGATAATCCTGGTGTT+TGG + chr3.2:69524173-69524192 MS.gene25898:CDS 45.0%
AGGTTCAAGGCAGATAATCC+TGG + chr3.2:69524166-69524185 MS.gene25898:CDS 45.0%
ATCTCATTGGAGCTGAAGAG+AGG - chr3.2:69521149-69521168 None:intergenic 45.0%
ATCTGCTCCTCAGTGATCTA+TGG + chr3.2:69521094-69521113 MS.gene25898:CDS 45.0%
ATGGTATGCAAAGAGCAGCA+GGG + chr3.2:69521001-69521020 MS.gene25898:CDS 45.0%
CACCACCAATCACTCCATTA+TGG + chr3.2:69523615-69523634 MS.gene25898:CDS 45.0%
CACTACCATTCCCATGTAAC+TGG - chr3.2:69523994-69524013 None:intergenic 45.0%
CCAACTATCAGAGCTGAAGT+TGG + chr3.2:69520298-69520317 MS.gene25898:CDS 45.0%
CCACAAGTAAGAGCTTCTGT+AGG - chr3.2:69524283-69524302 None:intergenic 45.0%
CCGTTACCGGTAGTTGTATT+TGG - chr3.2:69523917-69523936 None:intergenic 45.0%
CCTACAGAAGCTCTTACTTG+TGG + chr3.2:69524280-69524299 MS.gene25898:CDS 45.0%
GAAATTACATTGGGGTAGGG+TGG - chr3.2:69522857-69522876 None:intergenic 45.0%
GCAAACCAGTTACATGGGAA+TGG + chr3.2:69523986-69524005 MS.gene25898:CDS 45.0%
GCACCCACCTAAAAACACTA+AGG + chr3.2:69521304-69521323 MS.gene25898:intron 45.0%
GCACCCACCTAAAAACACTA+AGG - chr3.2:69521323-69521304 None:intergenic 45.0%
GGAACTGTTATTCACTGGCA+TGG + chr3.2:69520364-69520383 MS.gene25898:CDS 45.0%
GGAATCAATGGTCAGTTTCC+TGG + chr3.2:69520274-69520293 MS.gene25898:CDS 45.0%
GGCAGATAATCCTGGTGTTT+GGG + chr3.2:69524174-69524193 MS.gene25898:CDS 45.0%
GTTAAACCTTGTGGCGTGTT+TGG - chr3.2:69523548-69523567 None:intergenic 45.0%
TATGGTATGCAAAGAGCAGC+AGG + chr3.2:69521000-69521019 MS.gene25898:CDS 45.0%
TCATGTCCATGCAAATGCCA+TGG - chr3.2:69524025-69524044 None:intergenic 45.0%
TCTCTTCAGCTCCAATGAGA+TGG + chr3.2:69521148-69521167 MS.gene25898:CDS 45.0%
TGCATAGAGCATGTGGTGAT+GGG + chr3.2:69520253-69520272 MS.gene25898:CDS 45.0%
TGTTGAGGAGAAGAATCCTG+CGG - chr3.2:69523721-69523740 None:intergenic 45.0%
TTGCATAGAGCATGTGGTGA+TGG + chr3.2:69520252-69520271 MS.gene25898:CDS 45.0%
TTGTGTGCCATAGATCACTG+AGG - chr3.2:69521104-69521123 None:intergenic 45.0%
TTTGGAAATGTCTCTGGTGG+TGG - chr3.2:69523863-69523882 None:intergenic 45.0%
! ATCCTGCGGTGTGAGAATTT+TGG - chr3.2:69523707-69523726 None:intergenic 45.0%
! CAATGTCATCGACTGCGAAT+GGG - chr3.2:69523355-69523374 None:intergenic 45.0%
! CATAATGGAGTGATTGGTGG+TGG - chr3.2:69523617-69523636 None:intergenic 45.0%
! CCAACTTCAGCTCTGATAGT+TGG - chr3.2:69520301-69520320 None:intergenic 45.0%
! TCAATGTCATCGACTGCGAA+TGG - chr3.2:69523356-69523375 None:intergenic 45.0%
! TCCTTTGGAAATGTCTCTGG+TGG - chr3.2:69523866-69523885 None:intergenic 45.0%
!! CATGTGGTGATGGGAATCAA+TGG + chr3.2:69520262-69520281 MS.gene25898:CDS 45.0%
!! GGTGTCGTTTAGGAACATCT+TGG - chr3.2:69524314-69524333 None:intergenic 45.0%
!! TCTGACTCAATCGGTGTTAG+AGG + chr3.2:69523515-69523534 MS.gene25898:CDS 45.0%
!! TGATTGGTGGTGGTGAGTTT+GGG - chr3.2:69523607-69523626 None:intergenic 45.0%
!! TGTGAGAATTTTGGTGGCTG+TGG - chr3.2:69523698-69523717 None:intergenic 45.0%
!!! GGGTTTTGGGATATGGAGAA+GGG + chr3.2:69524050-69524069 MS.gene25898:CDS 45.0%
!!! TGGGTTTTGGGATATGGAGA+AGG + chr3.2:69524049-69524068 MS.gene25898:CDS 45.0%
ACACTGCTCATCAATGGACG+AGG + chr3.2:69522793-69522812 MS.gene25898:CDS 50.0%
AGCTCACGTGAACAAGAGGT+TGG + chr3.2:69521123-69521142 MS.gene25898:CDS 50.0%
CAAAGAGCAGCAGGGTTGTA+TGG + chr3.2:69521009-69521028 MS.gene25898:CDS 50.0%
CAATGAAACGCCCAAACACC+AGG - chr3.2:69524187-69524206 None:intergenic 50.0%
CACAAGCTCACGTGAACAAG+AGG + chr3.2:69521119-69521138 MS.gene25898:CDS 50.0%
CACCAAAATTCTCACACCGC+AGG + chr3.2:69523702-69523721 MS.gene25898:CDS 50.0%
TCACTGGCATGGAATCAGAC+AGG + chr3.2:69520375-69520394 MS.gene25898:CDS 50.0%
TCAGCTCCAATGAGATGGAC+CGG + chr3.2:69521153-69521172 MS.gene25898:CDS 50.0%
TTTGCAGCGTGGAACTCCTT+GGG + chr3.2:69520509-69520528 MS.gene25898:intron 50.0%
! CTGCGGTGTGAGAATTTTGG+TGG - chr3.2:69523704-69523723 None:intergenic 50.0%
! TGGCTGTGGAGTTCCCATTT+TGG - chr3.2:69523684-69523703 None:intergenic 50.0%
!! GTGATTGGTGGTGGTGAGTT+TGG - chr3.2:69523608-69523627 None:intergenic 50.0%
!! TGAAGAAGTGCCACTAGAGG+AGG - chr3.2:69520627-69520646 None:intergenic 50.0%
GGTTCACCGGTCCATCTCAT+TGG - chr3.2:69521162-69521181 None:intergenic 55.0%
GTTTGCAGCGTGGAACTCCT+TGG + chr3.2:69520508-69520527 MS.gene25898:intron 55.0%
! TCAGCTCTGATAGTTGGGCC+AGG - chr3.2:69520295-69520314 None:intergenic 55.0%
CGTGGAACTCCTTGGGCTGA+TGG + chr3.2:69520516-69520535 MS.gene25898:CDS 60.0%
GAGATGGACCGGTGAACCAC+AGG + chr3.2:69521164-69521183 MS.gene25898:CDS 60.0%
GCAGCAGTTCCATCAGCCCA+AGG - chr3.2:69520528-69520547 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69520133 69524342 69520133 ID=MS.gene25898
chr3.2 mRNA 69520133 69524342 69520133 ID=MS.gene25898.t1;Parent=MS.gene25898
chr3.2 exon 69520133 69520396 69520133 ID=MS.gene25898.t1.exon1;Parent=MS.gene25898.t1
chr3.2 CDS 69520133 69520396 69520133 ID=cds.MS.gene25898.t1;Parent=MS.gene25898.t1
chr3.2 exon 69520516 69520608 69520516 ID=MS.gene25898.t1.exon2;Parent=MS.gene25898.t1
chr3.2 CDS 69520516 69520608 69520516 ID=cds.MS.gene25898.t1;Parent=MS.gene25898.t1
chr3.2 exon 69520973 69521185 69520973 ID=MS.gene25898.t1.exon3;Parent=MS.gene25898.t1
chr3.2 CDS 69520973 69521185 69520973 ID=cds.MS.gene25898.t1;Parent=MS.gene25898.t1
chr3.2 exon 69522793 69522987 69522793 ID=MS.gene25898.t1.exon4;Parent=MS.gene25898.t1
chr3.2 CDS 69522793 69522987 69522793 ID=cds.MS.gene25898.t1;Parent=MS.gene25898.t1
chr3.2 exon 69523308 69524342 69523308 ID=MS.gene25898.t1.exon5;Parent=MS.gene25898.t1
chr3.2 CDS 69523308 69524342 69523308 ID=cds.MS.gene25898.t1;Parent=MS.gene25898.t1
Gene Sequence

>MS.gene25898

ATGAGGTTGTTGAGAGCCATTTTTGTATGGTGTATATGGTTTGGGATGTTTGAGCTATCATTTGGAGCAAAAGAAAGAAACTACAAGTTTGATGTTGAATACATATACAAAAAACCAGATTGCATAGAGCATGTGGTGATGGGAATCAATGGTCAGTTTCCTGGCCCAACTATCAGAGCTGAAGTTGGTGACACCCTTATCATTAATGTTACCAACAATCTTTCTACAGAAGGAACTGTTATTCACTGGCATGGAATCAGACAGGTAAATTTTCTTTCTCTTGTTCTAGTGGTTTTAGTTATGGAGCATATTACCAGTCTAATTGTAATTAGCATTACATTTTTTAATTTGTTGTTGATTAATATATATAATAATGTTTGCAGCGTGGAACTCCTTGGGCTGATGGAACTGCTGCAATATCTCAGTGTGCTATAAATCCAGGAGAAACTTTTCAATACAAATTCGAAGTTGACAAGGTGATTAATGAATTTCCTCCTCTAGTGGCACTTCTTCATATACATGTTGATTTTCATATAAATGTTGATCTTTCTGTTTTCATATACATGTTGATTTTGATCACTTATTTGTGCACTAGTGGCACTTCTTCATATTTCTCTTTTGTTTCCGTGTGTATTTTTTATTTTTTTTTCAAATTAATAGTTTCTAGCAATAACTCACATTGAGTTCAGATTAATAAGAAATTATGGATTTGAACCCACACCCTAACATATTAATGTTCCAGCCCATTTTTCATACAAACTGTACTGATCACGTAACCAACTGTGTGTTTAATTTAATATTGCTTTAACGGGTGGGTGTTTGATTATACTTACGGTGCAGCATGGTACATATTTCTATCATGGACACTATGGTATGCAAAGAGCAGCAGGGTTGTATGGTTCTCTAATAGTAGATTTACCAAAGAGGCATAAAGAGCCATTTCATTATGATGGTGAGTTCAATCTGCTCCTCAGTGATCTATGGCACACAAGCTCACGTGAACAAGAGGTTGGCCTCTCTTCAGCTCCAATGAGATGGACCGGTGAACCACAGGTATACATTCAACTTTTCATATATACAAAAATATATCTTCAAAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGGGTGCTGTTAACTTGCACCCACCTAAAAACACTAAGGTGCAAATTAACAAGCTGCACCTACTAGTTATTTACCAAAACACTCGTGTTTTTCTATTATAAACAAATATTATTTATGCTATTTACAAAAATGTCATTTTAATTAAAATAAAGTTTAATAGAGACAAAATGGTGTTTTAGTAAAGTACAAGTAGGTGCAACTTGTTAACTTGCACCTTAGTGTTTTTAGGTGGGTGCAAGTTAACAGCCCCCTATATATATATATATACACACACAAACAAACAAAAAGTCTTTCAAAATATATATATCATGTTCATTCAATTTACACCAGTGGAAATTAGTTTTTTGTCCAAATGAAAAGATGCGTTTTGCTAACTTAGACCTTCATATTTTTCGTGAAAATCCATTCGTGATATCTAGCATTTTTCATCCAATAAAACAACTGAAATAAATAAATCACAACACATTTGACGTACCTACAAATAAGAAAAGAAGATTGGAAAATAATAGACTCGTAATCCTATTCACCCAAAGAGAAAAAATATTTACAATAAAAAAGACTCATATTACACATACCAGGAAAAAAAGATTTATTCCAGATTAGAATGATCAAAATTAATAAGCAAATACCACATGATATAAACATACTCCCTCAGTTTTAAAATACATGTCCAATTTTTGCAATATGCACTATTCACTTGACTTACTTTGACCATACTTTTTAACTAATGTATAAATACAAATATTAGCATGTAAGATGTTGTTTGATTTGTCTCGACAAATATTTTCAAAATATTAAATTTTTATAATTTTTTACTTTAGATAATTAAAGATATTAACGGTCAAAATTGTACATTGACATATGTGAAGTGGTTGACTTGGACATGTATTTTGAAACGGAGGAAGTATAACATAATCTTTATTTTAAATATTTATAAAAAGAAGATTATTATTTGTTTTTATCATGTGGTATTTGCTTATTAATTATGATCACTCTCATATGAAATTAGTCTTTCTTTTTTGTTAAGTGGAATGATTCTTTTTTCTGTGTGAATATTTTTTCTTTGGGTCAATGGGGATGTGTTTTTATTAGTTCCCAATTTCCTATTCCTATTTTTATGTCACATTTTTTTAAATAAATTATTCTTTTATGTCACATGTGTGTGAGTTGTTTGTTTCAATTATTTTATTGGGTTTACAGATCAAATGCATAATATGATAAAAGGAAAACACAAATAAATGTGGATATGGTGCATATGTCACAAATAATCTATGCAGCAATCAATAAGAATTAGTCACATGAATAAATTTACAAGTGATACCCACAAACTAATTTTTATTCCACTAGCAAGTTAGTTTATGTTGCCAATTTTTATTGGTGGTTGAATAAATTCTCTACAATTTTTCAAATGTAGTATAGAGAAAACCATTATAAAAACGCATAGTTAATTTTATTTTTGAGAACATTTTTAACCTATCACACACTATGGTAATTAACTTTTTCAGACACTGCTCATCAATGGACGAGGACAATTCAATTGTTCCCTGGCAGCTAAATTTGTGAAGACCACCCTACCCCAATGTAATTTCAAAGGTGATGAAGAATGTGCACCATATATTCTTCAAGTGAAGCCAAACAAGACTTACAGAGTAAGGATTGCGAGTACAACGTCATTAGCTTCTCTCAACATGGCCATTTCAGTAAGTTTTTGATCTATTTCTAGTTATGACCATCTTTGTCGAAAAAAAAATATCATTTCATTGTTCGCACTTCCTGGAGATTTACATATTACTATAAGTTTAATTCACCAAAAACGAATGGAATAATTTAGGTAACGGTGAAAGTTAGAGTTATTAAAAAAAAAAAAGGTGTGAAAGGGATGTAGCTTAAGAAAGACTTGACTAATAGGCCAAGTAGAAGATCACACTCTCATCATCATTTAATTAGTTTCATCGTTAATTTCGCCATGGATCAGATCATTCTTCAATTAACAATGCCCCATAATTTGTCTATGCTGCAGAATCACAAACTTGTTGTAGTAGAAGCAGATGGAAACTACGTGCACCCATTCGCAGTCGATGACATTGATATCTACTCAGGTGAAACCTACTCAGTACTCATTAAAACAAACCAAGACCCCTCAAAAAACTACTGGATTGCAATTGGTGTTAGAGGTAGAAAGCCAAAAACACCACAAGCCATAACAATCCTTAACTACAAAACAATCTCTGACTCAATCGGTGTTAGAGGTAGAAAACCAAACACGCCACAAGGTTTAACAAACCTTAACAAAACAAGCTCTGTCTTTTCTGTTATCCCAAACTCACCACCACCAATCACTCCATTATGGAATGATACCAAACATAGCAAAGCATTCACAAAACAAATCATTTCCAAAATGGGAACTCCACAGCCACCAAAATTCTCACACCGCAGGATTCTTCTCCTCAACACACAAAACCTAATCGGTAGTTACACGAAATGGGCTATTAACAATGTTTCATTAACATTGCCAACAACACCTTACTTAGGATCAATCAAATATAAACTAAACAATACATTTGATAAAAATCCACCACCAGAGACATTTCCAAAGGATTATGACATATTTAAAAATCCAGTTAATCCAAATACAACTACCGGTAACGGTGTTTACATGTTTAATAAAGATGAGATTGTTGATGTGATACTACAGAATGCAAACCAGTTACATGGGAATGGTAGTGAAATTCATCCATGGCATTTGCATGGACATGATTTTTGGGTTTTGGGATATGGAGAAGGGAAATTCAAACCAAGTGATGCGAAAAGTTTCAATTTGACACGTGCACCTTTAAGGAACACCGTGGTTGTATTTCCATATGGTTGGACTGCATTAAGGTTCAAGGCAGATAATCCTGGTGTTTGGGCGTTTCATTGTCACATTGAACAACATTTGCATATGGGAATGGGTGTTATTTTTGCTGAGGGTGTTCACAAAATTAGGAAAGTACCTACAGAAGCTCTTACTTGTGGTGCTACAGCCAAGATGTTCCTAAACGACACCCTTCATTGA
Protein sequence

>MS.gene25898.t1

MRLLRAIFVWCIWFGMFELSFGAKERNYKFDVEYIYKKPDCIEHVVMGINGQFPGPTIRAEVGDTLIINVTNNLSTEGTVIHWHGIRQRGTPWADGTAAISQCAINPGETFQYKFEVDKHGTYFYHGHYGMQRAAGLYGSLIVDLPKRHKEPFHYDGEFNLLLSDLWHTSSREQEVGLSSAPMRWTGEPQTLLINGRGQFNCSLAAKFVKTTLPQCNFKGDEECAPYILQVKPNKTYRVRIASTTSLASLNMAISNHKLVVVEADGNYVHPFAVDDIDIYSGETYSVLIKTNQDPSKNYWIAIGVRGRKPKTPQAITILNYKTISDSIGVRGRKPNTPQGLTNLNKTSSVFSVIPNSPPPITPLWNDTKHSKAFTKQIISKMGTPQPPKFSHRRILLLNTQNLIGSYTKWAINNVSLTLPTTPYLGSIKYKLNNTFDKNPPPETFPKDYDIFKNPVNPNTTTGNGVYMFNKDEIVDVILQNANQLHGNGSEIHPWHLHGHDFWVLGYGEGKFKPSDAKSFNLTRAPLRNTVVVFPYGWTALRFKADNPGVWAFHCHIEQHLHMGMGVIFAEGVHKIRKVPTEALTCGATAKMFLNDTLH