AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004011

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004011.t1 MTR_4g064530 98.126 587 9 1 1 587 1 585 0.0 1177
MS.gene004011.t1 MTR_4g064530 97.459 551 12 1 1 551 1 549 0.0 1087
MS.gene004011.t1 MTR_7g060460 73.883 582 140 6 14 587 4 581 0.0 894
MS.gene004011.t1 MTR_7g058690 72.696 586 145 6 11 587 1 580 0.0 878
MS.gene004011.t1 MTR_7g458880 70.748 588 157 5 7 587 1 580 0.0 870
MS.gene004011.t1 MTR_7g062250 70.833 576 155 5 16 587 12 578 0.0 865
MS.gene004011.t1 MTR_7g062310 67.504 557 172 4 37 587 40 593 0.0 791
MS.gene004011.t1 MTR_5g069680 55.191 549 230 7 41 587 24 558 0.0 628
MS.gene004011.t1 MTR_3g462760 54.927 548 232 5 41 587 23 556 0.0 628
MS.gene004011.t1 MTR_4g015120 55.229 545 232 5 44 587 24 557 0.0 626
MS.gene004011.t1 MTR_5g020600 53.734 549 239 6 41 587 31 566 0.0 622
MS.gene004011.t1 MTR_5g020620 52.083 576 258 8 14 587 2 561 0.0 615
MS.gene004011.t1 MTR_5g083360 51.473 577 259 10 20 587 3 567 0.0 594
MS.gene004011.t1 MTR_5g081810 52.222 540 243 5 49 587 4 529 0.0 587
MS.gene004011.t1 MTR_5g073210 49.828 582 262 12 13 587 2 560 0.0 548
MS.gene004011.t1 MTR_3g071890 48.944 568 245 12 20 564 13 558 0.0 529
MS.gene004011.t1 MTR_8g027375 47.341 583 276 11 21 587 4 571 0.0 527
MS.gene004011.t1 MTR_2g008330 45.985 548 286 6 44 587 38 579 0.0 523
MS.gene004011.t1 MTR_7g065980 44.974 567 295 8 31 587 12 571 1.52e-174 506
MS.gene004011.t1 MTR_7g065970 47.569 576 278 11 25 587 6 570 9.53e-171 497
MS.gene004011.t1 MTR_4g019225 45.683 556 279 7 45 587 27 572 2.43e-170 496
MS.gene004011.t1 MTR_3g101635 46.235 571 285 10 13 577 2 556 7.98e-168 489
MS.gene004011.t1 MTR_3g101640 45.283 583 295 12 13 587 5 571 1.64e-167 489
MS.gene004011.t1 MTR_8g091390 31.083 563 325 18 44 578 34 561 5.58e-66 226
MS.gene004011.t1 MTR_5g081790 41.958 286 149 6 269 553 159 428 7.36e-62 211
MS.gene004011.t1 MTR_5g081790 51.136 176 73 2 19 187 5 174 1.97e-55 194
MS.gene004011.t1 MTR_3g078700 31.206 564 312 19 45 587 27 535 1.49e-61 213
MS.gene004011.t1 MTR_3g078740 31.336 584 329 21 40 578 22 578 2.36e-60 211
MS.gene004011.t1 MTR_3g078730 30.922 553 315 20 44 578 25 528 5.33e-59 206
MS.gene004011.t1 MTR_4g101650 27.667 553 336 14 19 552 7 514 6.65e-49 179
MS.gene004011.t1 MTR_3g008580 26.582 553 347 14 19 552 4 516 1.26e-46 173
MS.gene004011.t1 MTR_8g073840 44.330 194 69 4 37 229 23 178 9.96e-46 163
MS.gene004011.t1 MTR_1g090707 28.991 545 326 19 58 587 38 536 1.26e-42 162
MS.gene004011.t1 MTR_7g058670 74.074 81 20 1 101 180 13 93 1.04e-38 137
MS.gene004011.t1 MTR_7g051440 26.316 532 321 18 45 552 30 514 9.65e-36 142
MS.gene004011.t1 MTR_1g026410 24.908 546 366 14 44 587 29 532 2.76e-35 140
MS.gene004011.t1 MTR_3g115050 24.162 567 343 15 46 587 29 533 2.62e-33 134
MS.gene004011.t1 MTR_4g049390 25.048 519 319 14 44 547 27 490 2.28e-32 131
MS.gene004011.t1 MTR_4g075690 24.665 523 327 16 44 553 30 498 8.76e-31 127
MS.gene004011.t1 MTR_2g088080 24.668 527 311 14 44 547 30 493 1.07e-29 123
MS.gene004011.t1 MTR_4g048350 25.000 520 317 17 44 547 27 489 1.86e-29 122
MS.gene004011.t1 MTR_3g083450 25.094 530 352 15 45 568 27 517 2.09e-29 122
MS.gene004011.t1 MTR_3g083430 24.578 533 351 18 45 568 26 516 3.09e-29 122
MS.gene004011.t1 MTR_5g018270 25.545 505 330 14 44 547 31 490 6.96e-29 121
MS.gene004011.t1 MTR_3g083390 24.627 536 347 16 45 568 26 516 1.36e-28 120
MS.gene004011.t1 MTR_3g083400 24.440 536 348 16 45 568 26 516 2.00e-28 120
MS.gene004011.t1 MTR_3g083440 24.440 536 348 16 45 568 26 516 2.57e-28 119
MS.gene004011.t1 MTR_8g479380 24.376 521 346 16 33 547 13 491 1.34e-27 117
MS.gene004011.t1 MTR_4g083210 24.717 530 340 16 45 563 26 507 1.75e-27 117
MS.gene004011.t1 MTR_8g479390 23.892 519 324 16 44 547 31 493 2.71e-26 113
MS.gene004011.t1 MTR_3g083410 24.612 516 332 16 65 568 10 480 2.79e-26 113
MS.gene004011.t1 MTR_4g132320 24.706 510 332 16 44 547 31 494 1.52e-25 111
MS.gene004011.t1 MTR_4g075690 28.358 268 179 6 44 310 30 285 3.12e-25 109
MS.gene004011.t1 MTR_4g083190 25.000 532 356 17 19 547 6 497 8.87e-24 105
MS.gene004011.t1 MTR_2g088080 24.086 465 283 14 98 547 3 412 3.78e-20 94.0
MS.gene004011.t1 MTR_2g436410 56.338 71 27 2 388 457 5 72 1.48e-15 73.6
MS.gene004011.t1 MTR_3g079660 42.683 82 45 1 497 578 5 84 1.65e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004011.t1 AT2G29130 70.312 576 157 6 15 587 9 573 0.0 860
MS.gene004011.t1 AT5G60020 69.603 579 155 6 20 587 9 577 0.0 852
MS.gene004011.t1 AT5G60020 71.846 547 141 5 52 587 1 545 0.0 834
MS.gene004011.t1 AT5G60020 69.274 537 144 6 20 545 9 535 0.0 780
MS.gene004011.t1 AT2G38080 54.482 569 241 6 19 587 8 558 0.0 637
MS.gene004011.t1 AT5G58910 52.205 567 251 8 25 587 17 567 0.0 611
MS.gene004011.t1 AT5G03260 52.531 573 250 9 18 587 4 557 0.0 611
MS.gene004011.t1 AT5G58910 52.205 567 250 9 25 587 17 566 0.0 607
MS.gene004011.t1 AT2G40370 51.515 594 255 11 11 587 3 580 0.0 595
MS.gene004011.t1 AT5G05390 53.621 580 240 11 16 587 7 565 0.0 593
MS.gene004011.t1 AT5G01190 52.348 575 254 8 15 587 2 558 0.0 591
MS.gene004011.t1 AT1G18140 50.444 563 258 10 40 587 25 581 0.0 578
MS.gene004011.t1 AT5G58910 51.481 540 241 7 52 587 1 523 0.0 572
MS.gene004011.t1 AT2G30210 50.172 580 268 9 16 587 4 570 0.0 571
MS.gene004011.t1 AT5G01190 51.478 575 245 9 15 587 2 544 0.0 568
MS.gene004011.t1 AT5G07130 50.271 553 255 11 46 587 26 569 0.0 530
MS.gene004011.t1 AT2G46570 45.423 579 287 11 20 587 9 569 0.0 528
MS.gene004011.t1 AT5G09360 44.406 572 292 12 22 587 18 569 1.48e-172 501
MS.gene004011.t1 AT3G09220 46.801 547 272 8 32 570 15 550 2.28e-172 501
MS.gene004011.t1 AT5G48100 45.505 545 277 11 42 577 20 553 1.05e-163 479
MS.gene004011.t1 AT5G07130 50.304 493 225 11 106 587 1 484 1.73e-158 462
MS.gene004011.t1 AT5G07130 50.304 493 225 11 106 587 1 484 1.73e-158 462
MS.gene004011.t1 AT5G01050 43.134 568 296 10 32 587 16 568 3.30e-157 463
MS.gene004011.t1 AT2G46570 45.309 501 246 10 98 587 1 484 5.28e-155 454
MS.gene004011.t1 AT5G01040 42.756 566 299 10 32 587 16 566 2.97e-154 455
MS.gene004011.t1 AT3G09220 48.052 462 222 7 117 570 2 453 1.03e-143 424
MS.gene004011.t1 AT4G39830 30.823 571 324 19 44 578 36 571 2.99e-69 234
MS.gene004011.t1 AT5G21105 30.653 597 346 19 17 577 9 573 3.06e-68 232
MS.gene004011.t1 AT5G21100 30.036 556 313 17 59 578 39 554 3.61e-67 229
MS.gene004011.t1 AT5G21105 30.851 564 321 21 17 545 9 538 1.13e-59 208
MS.gene004011.t1 AT4G39830 29.746 511 285 17 105 578 3 476 3.87e-50 181
MS.gene004011.t1 AT4G39830 29.842 506 281 17 110 578 6 474 9.44e-50 179
MS.gene004011.t1 AT4G12420 27.186 526 324 13 46 552 27 512 3.24e-47 174
MS.gene004011.t1 AT4G12420 27.186 526 324 13 46 552 27 512 3.24e-47 174
MS.gene004011.t1 AT4G39830 28.824 510 278 16 105 578 3 463 1.81e-45 167
MS.gene004011.t1 AT5G51480 28.107 523 313 16 46 547 30 510 1.06e-44 167
MS.gene004011.t1 AT1G75790 28.383 532 337 17 20 547 4 495 1.76e-44 166
MS.gene004011.t1 AT4G25240 27.289 557 334 17 15 547 1 510 5.13e-44 166
MS.gene004011.t1 AT4G25240 27.289 557 334 17 15 547 1 510 5.13e-44 166
MS.gene004011.t1 AT5G21105 32.033 359 208 10 228 577 51 382 3.83e-38 145
MS.gene004011.t1 AT1G21860 25.185 540 344 14 23 553 8 496 5.10e-38 148
MS.gene004011.t1 AT5G48450 26.923 520 339 17 46 561 31 513 1.92e-37 147
MS.gene004011.t1 AT1G21850 24.382 566 345 17 1 553 1 496 2.68e-37 146
MS.gene004011.t1 AT5G48450 27.559 508 327 17 58 561 43 513 4.84e-37 146
MS.gene004011.t1 AT1G76160 25.271 554 340 17 13 553 4 496 5.06e-33 133
MS.gene004011.t1 AT4G22010 25.668 561 344 20 44 587 26 530 1.83e-31 129
MS.gene004011.t1 AT1G41830 23.894 565 386 14 24 587 10 531 4.78e-31 127
MS.gene004011.t1 AT5G66920 24.324 518 330 15 44 551 37 502 5.48e-31 127
MS.gene004011.t1 AT3G13390 25.540 509 335 14 45 547 29 499 1.80e-27 117
MS.gene004011.t1 AT4G28090 23.107 515 351 13 43 553 27 500 1.98e-27 117
MS.gene004011.t1 AT4G37160 24.025 487 328 13 62 547 49 494 5.62e-26 112
MS.gene004011.t1 AT1G55570 24.852 507 341 15 45 547 30 500 9.10e-26 112
MS.gene004011.t1 AT3G13400 25.000 504 343 14 45 547 28 497 9.74e-26 112
MS.gene004011.t1 AT3G13400 25.000 504 343 14 45 547 28 497 9.74e-26 112
MS.gene004011.t1 AT4G38420 21.722 511 364 13 44 553 30 505 1.63e-25 111
MS.gene004011.t1 AT2G23630 25.462 487 320 14 62 547 48 492 2.65e-24 107
MS.gene004011.t1 AT2G23630 25.462 487 320 14 62 547 53 497 3.01e-24 107
MS.gene004011.t1 AT1G55560 29.455 275 183 7 62 335 44 308 1.78e-23 105
MS.gene004011.t1 AT4G38420 22.645 499 326 15 44 529 30 481 3.92e-21 97.4
MS.gene004011.t1 AT4G38420 26.087 276 193 6 44 318 30 295 1.90e-20 94.7
MS.gene004011.t1 AT4G37160 27.016 248 169 4 62 308 49 285 4.79e-20 93.6

Find 150 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCACATTTGCAGGGTATTT+TGG 0.173773 4.3:-39426501 None:intergenic
TCTGATGCCTACACCATTAA+TGG 0.176037 4.3:+39425459 MS.gene004011:CDS
ATTGCAGGATACAAGCATTT+TGG 0.187461 4.3:+39426839 MS.gene004011:CDS
TTTGAATTTGAACTATTATT+TGG 0.212461 4.3:-39426603 None:intergenic
CTTCTCCAACAACATTTCTT+TGG 0.228697 4.3:+39426675 MS.gene004011:CDS
CATTTCTTTGGAACTAATAA+TGG 0.256030 4.3:+39426687 MS.gene004011:CDS
AACCTCCCCTGGATTTGTTT+TGG 0.259173 4.3:+39424281 MS.gene004011:CDS
TTTGTTGTTGGTCAAGGTTT+TGG 0.271917 4.3:+39426903 MS.gene004011:CDS
TAACTCTGACCTGTTTGAAT+TGG 0.297459 4.3:-39425007 None:intergenic
CTTTGATGTGCACCTTAGTT+GGG 0.307238 4.3:+39427489 MS.gene004011:CDS
AATACTGTTGCTGTGCCTTC+TGG 0.309543 4.3:+39426984 MS.gene004011:CDS
AATGTGGCATTTGGGTAATT+TGG 0.312994 4.3:-39426285 None:intergenic
ACGAGAAGGGGACAGATTAT+TGG 0.313664 4.3:+39424582 MS.gene004011:CDS
CAATGCAGGCTGATATTGTT+TGG 0.314456 4.3:-39424625 None:intergenic
TACACCATTAATGGGTTTCC+TGG 0.319870 4.3:+39425468 MS.gene004011:CDS
TTGGTGTAGTAATCAACATT+TGG 0.322522 4.3:-39427587 None:intergenic
TGGTGTAGTAATCAACATTT+GGG 0.326399 4.3:-39427586 None:intergenic
TCAACAAGATTAAACTTTGC+AGG 0.331305 4.3:-39426948 None:intergenic
AGTCATCCTTTGCATCTTCA+TGG 0.334535 4.3:+39426870 MS.gene004011:CDS
AAGGAAGTCCCCATTCTCTT+TGG 0.338186 4.3:+39425169 MS.gene004011:CDS
ACCTGAACTCTAGTGTTATA+TGG 0.345887 4.3:-39426813 None:intergenic
GCTTGTGCAATTACAGCTTC+AGG 0.346802 4.3:-39425408 None:intergenic
TGGTTAAGAGCTACTGTCTA+TGG 0.350969 4.3:+39425091 MS.gene004011:CDS
GGATTTAATTTCTTTGTTGT+TGG 0.356145 4.3:+39426891 MS.gene004011:CDS
TTACTAACCAAAGTATTGTT+TGG 0.366291 4.3:-39426768 None:intergenic
TTGCTGTGCCTTCTGGTGGT+TGG 0.366686 4.3:+39426991 MS.gene004011:CDS
CTGATGCCTACACCATTAAT+GGG 0.371595 4.3:+39425460 MS.gene004011:CDS
TATTTGGTCCTTGACAAGTT+TGG 0.374143 4.3:-39426587 None:intergenic
AGGTCCAGGAAACCCATTAA+TGG 0.377126 4.3:-39425472 None:intergenic
AACTCTGACCTGTTTGAATT+GGG 0.379719 4.3:-39425006 None:intergenic
AACAAAATTAGTATCATTGA+TGG 0.379750 4.3:-39426442 None:intergenic
CAACATTTGGGGAGATCCTT+AGG 0.380323 4.3:-39427574 None:intergenic
AGCATGTAGAATGTGGCATT+TGG 0.380488 4.3:-39426294 None:intergenic
GACAAAGAGGAACTCTCTTC+TGG 0.381182 4.3:+39425053 MS.gene004011:CDS
AAAAGCATGGTTACCGTGAA+TGG 0.383433 4.3:+39424532 MS.gene004011:CDS
AGGGGACAGATTATTGGTTA+AGG 0.383575 4.3:+39424588 MS.gene004011:CDS
GTACCCTGCCCTGTGAAATA+TGG 0.385825 4.3:-39426324 None:intergenic
TGCTGTGCCTTCTGGTGGTT+GGG 0.390745 4.3:+39426992 MS.gene004011:CDS
TAGAGTTCAGGTTATATTGC+AGG 0.393539 4.3:+39426824 MS.gene004011:CDS
TACCGTGAATGGGAAGTTCC+CGG 0.400570 4.3:+39424543 MS.gene004011:CDS
GAGAATGGGGACTTCCTTGT+AGG 0.406530 4.3:-39425164 None:intergenic
GTGTAGGCATCAGAAACATT+TGG 0.413299 4.3:-39425450 None:intergenic
GCATGTAGAATGTGGCATTT+GGG 0.416680 4.3:-39426293 None:intergenic
ACTTTGATGTGCACCTTAGT+TGG 0.416806 4.3:+39427488 MS.gene004011:CDS
ACCTTCCTCTTAACCTCCCC+TGG 0.418136 4.3:+39424270 MS.gene004011:CDS
GTTGGGGATTGAGGATGGCT+TGG 0.419155 4.3:+39427506 MS.gene004011:CDS
ACCATATAACACTAGAGTTC+AGG 0.422189 4.3:+39426812 MS.gene004011:CDS
GTATAGTTAAACGGCCTTAA+CGG 0.423945 4.3:-39426735 None:intergenic
TTCGACAATTCCACAGTAGC+TGG 0.425573 4.3:+39426348 MS.gene004011:CDS
AATTTCTTTGTTGTTGGTCA+AGG 0.431250 4.3:+39426897 MS.gene004011:CDS
TTAAGGCCGTTTAACTATAC+AGG 0.432215 4.3:+39426738 MS.gene004011:CDS
GATAAGATTCATTGTGCCTT+GGG 0.433565 4.3:-39425129 None:intergenic
CAAATCCAGGGGAGGTTAAG+AGG 0.435761 4.3:-39424275 None:intergenic
GTGTACAACTTCACCATTGT+TGG 0.440870 4.3:+39425031 MS.gene004011:CDS
TTCATATGTACCAAAGAGAA+TGG 0.444243 4.3:-39425179 None:intergenic
AACGGCCTTAACGGTACAAC+CGG 0.445112 4.3:-39426726 None:intergenic
TGTAGGCATCAGAAACATTT+GGG 0.450843 4.3:-39425449 None:intergenic
GCAACAGTATTCCTCTCAAC+AGG 0.458149 4.3:-39426972 None:intergenic
AACTCCTCCAAACAATACTT+TGG 0.459111 4.3:+39426761 MS.gene004011:CDS
TGCACCTTAGTTGGGGATTG+AGG 0.460367 4.3:+39427497 MS.gene004011:CDS
GGATAAGATTCATTGTGCCT+TGG 0.462220 4.3:-39425130 None:intergenic
CCTTAGTTGGGGATTGAGGA+TGG 0.469162 4.3:+39427501 MS.gene004011:CDS
TCATTGATGGCTGGAAGCAC+AGG 0.471762 4.3:-39426429 None:intergenic
AAAATAGTTCTTACCAATGC+AGG 0.472186 4.3:-39424639 None:intergenic
GGAGTTCCTGTATAGTTAAA+CGG 0.473322 4.3:-39426744 None:intergenic
TCATATGTACCAAAGAGAAT+GGG 0.475158 4.3:-39425178 None:intergenic
TCTGGCATGCTCATTTCTCA+TGG 0.475847 4.3:+39425071 MS.gene004011:CDS
AACAATACTTTGGTTAGTAA+TGG 0.478654 4.3:+39426771 MS.gene004011:CDS
TAGTTCCAAAGAAATGTTGT+TGG 0.480695 4.3:-39426680 None:intergenic
ATTATTTCTGCAGATAAGGT+TGG 0.482733 4.3:+39424483 MS.gene004011:intron
ATTGCACAAGCTCTGCAGAC+AGG 0.483287 4.3:+39425420 MS.gene004011:CDS
ACTCAATGCCCAATTCAAAC+AGG 0.483829 4.3:+39424998 MS.gene004011:CDS
CGATTCCTTGCTGACAATCC+AGG 0.486079 4.3:+39427023 MS.gene004011:CDS
GAAGTAGCCAAAACAAATCC+AGG 0.492773 4.3:-39424288 None:intergenic
TTCACCATTGTTGGACAAAG+AGG 0.495747 4.3:+39425040 MS.gene004011:CDS
GGGCATTGAGTTATGTATGA+TGG 0.498399 4.3:-39424986 None:intergenic
CCTTCTCGTACAACAATTCG+AGG 0.499048 4.3:-39424568 None:intergenic
TGGTCCAAGTATGATGGTAT+TGG 0.499764 4.3:-39426229 None:intergenic
GTATGGTTGAAACAGGTGTT+TGG 0.506981 4.3:+39427452 MS.gene004011:intron
ACCCGGGAACTTCCCATTCA+CGG 0.507216 4.3:-39424545 None:intergenic
GAGCAATTGTAAAGAGGTCC+AGG 0.507787 4.3:-39425486 None:intergenic
CTTCGTAGTGGTTGGTCGGA+TGG 0.508027 4.3:+39424962 MS.gene004011:CDS
CTCGAATTGTTGTACGAGAA+GGG 0.509000 4.3:+39424569 MS.gene004011:CDS
TGTTGGAGAAGTGCTATAGA+AGG 0.510179 4.3:-39426663 None:intergenic
CCTCTTTACAATTGCTCCAA+AGG 0.511152 4.3:+39425492 MS.gene004011:CDS
AAAGCATGGTTACCGTGAAT+GGG 0.513288 4.3:+39424533 MS.gene004011:CDS
AAAATTGCCTCCTCCTCCTA+AGG 0.514093 4.3:+39427558 MS.gene004011:CDS
ATGGCTTGGATTGTTGAGGA+TGG 0.516858 4.3:+39427520 MS.gene004011:CDS
ATTCATTGTGCCTTGGGAGA+AGG 0.517082 4.3:-39425123 None:intergenic
AGAATGGGGACTTCCTTGTA+GGG 0.517590 4.3:-39425163 None:intergenic
TTTGATGTGCACCTTAGTTG+GGG 0.517799 4.3:+39427490 MS.gene004011:CDS
TAGACCATATTTCACAGGGC+AGG 0.518387 4.3:+39426320 MS.gene004011:CDS
GACAGATACATACAAATTGA+AGG 0.527305 4.3:+39426077 MS.gene004011:intron
CTAGCTAGACCATATTTCAC+AGG 0.529517 4.3:+39426315 MS.gene004011:CDS
TACAAATTGAAGGTGAAGCC+AGG 0.530121 4.3:+39426087 MS.gene004011:CDS
GAGGATGGCTTGGATTGTTG+AGG 0.530967 4.3:+39427516 MS.gene004011:CDS
AGAACATTTGTGGTTTGACC+TGG 0.536892 4.3:-39426249 None:intergenic
CTAACCAAAGTATTGTTTGG+AGG 0.538509 4.3:-39426765 None:intergenic
TTGCACAAGCTCTGCAGACA+GGG 0.538737 4.3:+39425421 MS.gene004011:CDS
ACCGTGAATGGGAAGTTCCC+GGG 0.543276 4.3:+39424544 MS.gene004011:CDS
GAGTCCAATACCATCATACT+TGG 0.543746 4.3:+39426225 MS.gene004011:CDS
CCTCGAATTGTTGTACGAGA+AGG 0.546915 4.3:+39424568 MS.gene004011:CDS
CCATCCTCAATCCCCAACTA+AGG 0.552441 4.3:-39427501 None:intergenic
ACTGTTGCTGTGCCTTCTGG+TGG 0.553231 4.3:+39426987 MS.gene004011:CDS
CCTTTGGAGCAATTGTAAAG+AGG 0.556173 4.3:-39425492 None:intergenic
TCTGCAGATAAGGTTGGCAA+AGG 0.561585 4.3:+39424489 MS.gene004011:intron
TGTCACTGTTCCATTATCAA+AGG 0.562747 4.3:+39424218 MS.gene004011:CDS
GGGAGATCCTTAGGAGGAGG+AGG 0.564134 4.3:-39427565 None:intergenic
CAACAAGATTAAACTTTGCA+GGG 0.564792 4.3:-39426947 None:intergenic
CGTACAACAATTCGAGGACC+CGG 0.566305 4.3:-39424562 None:intergenic
TGAGACAACTTCGTAGTGGT+TGG 0.574177 4.3:+39424954 MS.gene004011:CDS
TTAAATCCATGAAGATGCAA+AGG 0.575251 4.3:-39426876 None:intergenic
TTCTAAAATTCCAGCTACTG+TGG 0.577452 4.3:-39426358 None:intergenic
TTGGGGAGATCCTTAGGAGG+AGG 0.578506 4.3:-39427568 None:intergenic
AGGAAACCCATTAATGGTGT+AGG 0.579472 4.3:-39425466 None:intergenic
AAATTAGTATCATTGATGGC+TGG 0.579956 4.3:-39426438 None:intergenic
AATCACCTGGATTGTCAGCA+AGG 0.580919 4.3:-39427028 None:intergenic
CAAACAATATCAGCCTGCAT+TGG 0.581284 4.3:+39424626 MS.gene004011:CDS
TTGACCTGGTCCAAGTATGA+TGG 0.586103 4.3:-39426235 None:intergenic
GACCTCTCATCCTTCTCCCA+AGG 0.596454 4.3:+39425113 MS.gene004011:CDS
TCCAGGGGAGGTTAAGAGGA+AGG 0.601150 4.3:-39424271 None:intergenic
ACAACTTCGTAGTGGTTGGT+CGG 0.604046 4.3:+39424958 MS.gene004011:CDS
AAGTAGCCAAAACAAATCCA+GGG 0.606149 4.3:-39424287 None:intergenic
ATCTTGTTGATCCTGTTGAG+AGG 0.606779 4.3:+39426961 MS.gene004011:CDS
AGTTCCTCTTTGTCCAACAA+TGG 0.608427 4.3:-39425044 None:intergenic
AGACCATATTTCACAGGGCA+GGG 0.613998 4.3:+39426321 MS.gene004011:CDS
TCGAATTGTTGTACGAGAAG+GGG 0.619585 4.3:+39424570 MS.gene004011:CDS
ATCGCGACCCAACCACCAGA+AGG 0.630640 4.3:-39426999 None:intergenic
CATTTGGGGAGATCCTTAGG+AGG 0.640212 4.3:-39427571 None:intergenic
TGCCTTGGGAGAAGGATGAG+AGG 0.645507 4.3:-39425115 None:intergenic
ATGATGGTATTGGACTCGAA+TGG 0.647830 4.3:-39426219 None:intergenic
CATATGTACCAAAGAGAATG+GGG 0.650276 4.3:-39425177 None:intergenic
TGGACTAGTTCCTAGTCCAA+CGG 0.652330 4.3:-39426553 None:intergenic
GACAAATATGCATAGGTGAG+TGG 0.653174 4.3:+39425373 MS.gene004011:intron
AGCCAAAACAAATCCAGGGG+AGG 0.654034 4.3:-39424283 None:intergenic
GTACAACAATTCGAGGACCC+GGG 0.658530 4.3:-39424561 None:intergenic
AAATATGCATAGGTGAGTGG+TGG 0.659012 4.3:+39425376 MS.gene004011:intron
TCTAGCTAGCATGTAGAATG+TGG 0.659413 4.3:-39426301 None:intergenic
TGCACAAGCTCTGCAGACAG+GGG 0.660853 4.3:+39425422 MS.gene004011:CDS
TAAGGTTGGCAAAGGTTACG+AGG 0.665174 4.3:+39424497 MS.gene004011:CDS
AAGATGCATCTGCTTCGACA+AGG 0.668532 4.3:-39426188 None:intergenic
GCACAAGCTCTGCAGACAGG+GGG 0.670081 4.3:+39425423 MS.gene004011:CDS
AACTAAGGTGCACATCAAAG+TGG 0.677837 4.3:-39427486 None:intergenic
TAGCTAGACCATATTTCACA+GGG 0.683532 4.3:+39426316 MS.gene004011:CDS
AATACCATCATACTTGGACC+AGG 0.697209 4.3:+39426231 MS.gene004011:CDS
TAGTAATGGTACAAAGACAG+TGG 0.701875 4.3:+39426785 MS.gene004011:CDS
AGTAGCCAAAACAAATCCAG+GGG 0.722267 4.3:-39424286 None:intergenic
AGGGGAGGTTAAGAGGAAGG+TGG 0.734018 4.3:-39424268 None:intergenic
GGTGTAGTAATCAACATTTG+GGG 0.734375 4.3:-39427585 None:intergenic
GGTGTGAGACAACTTCGTAG+TGG 0.741558 4.3:+39424950 MS.gene004011:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTATATGATATATAATATT+TGG - chr4.3:39427326-39427345 None:intergenic 10.0%
!! ATAAATAATTTATTATACTC+TGG + chr4.3:39425228-39425247 MS.gene004011:intron 10.0%
!! TTTATTATAAATTTATACTG+AGG - chr4.3:39425213-39425232 None:intergenic 10.0%
!! AAAATAGTATATGATATGAA+AGG + chr4.3:39425326-39425345 MS.gene004011:intron 15.0%
!! TTAAGTGATAAATATTTGAA+AGG - chr4.3:39424382-39424401 None:intergenic 15.0%
!! TTTGAATTTGAACTATTATT+TGG - chr4.3:39426606-39426625 None:intergenic 15.0%
!!! TTTTTATTTGTTTGATCATT+TGG - chr4.3:39426393-39426412 None:intergenic 15.0%
!! AACAAAATTAGTATCATTGA+TGG - chr4.3:39426445-39426464 None:intergenic 20.0%
!! AATAATTCATTTCATGCATA+TGG - chr4.3:39424878-39424897 None:intergenic 20.0%
!! ACTACATATGTTATACAATT+TGG - chr4.3:39424424-39424443 None:intergenic 20.0%
!! TGTACTAATATTGACAATAA+TGG + chr4.3:39424897-39424916 MS.gene004011:intron 20.0%
!!! AAATTAGTTTGTAAAATGAG+AGG - chr4.3:39425960-39425979 None:intergenic 20.0%
!!! AACTAATAATGGTTTTACAA+CGG + chr4.3:39426698-39426717 MS.gene004011:CDS 20.0%
!!! AAGTTTTTGTCAATAGTTTT+AGG - chr4.3:39426525-39426544 None:intergenic 20.0%
!!! AGTTTTTAATAGAACATTTG+TGG - chr4.3:39426262-39426281 None:intergenic 20.0%
!!! TTTTATTATTTCTGCAGATA+AGG + chr4.3:39424479-39424498 MS.gene004011:intron 20.0%
!!! TTTTTGAAAGATGAATTGAT+TGG + chr4.3:39426037-39426056 MS.gene004011:intron 20.0%
! AACAATACTTTGGTTAGTAA+TGG + chr4.3:39426771-39426790 MS.gene004011:CDS 25.0%
! ATATTGTATGTTGTACATCA+TGG + chr4.3:39424837-39424856 MS.gene004011:intron 25.0%
! CATATGTTATACAATTTGGT+AGG - chr4.3:39424420-39424439 None:intergenic 25.0%
! GAATAATGACTAGAATTGAT+TGG + chr4.3:39426006-39426025 MS.gene004011:intron 25.0%
! TATCTTTGACAAATATGCAT+AGG + chr4.3:39425366-39425385 MS.gene004011:intron 25.0%
!! AACATTTTCTTTATCCTAAG+TGG - chr4.3:39425912-39425931 None:intergenic 25.0%
!! ACTATTTTCATAAACTATCC+TGG + chr4.3:39427183-39427202 MS.gene004011:intron 25.0%
!! GGATTTAATTTCTTTGTTGT+TGG + chr4.3:39426891-39426910 MS.gene004011:CDS 25.0%
!!! AAAAACTTTTTCTTTACCGT+TGG + chr4.3:39426537-39426556 MS.gene004011:CDS 25.0%
!!! AAACTAAGTGTTGAGAAAAT+AGG + chr4.3:39424725-39424744 MS.gene004011:intron 25.0%
!!! ATCTATTTGCTATGAATGTA+TGG + chr4.3:39427435-39427454 MS.gene004011:intron 25.0%
!!! ATTGGTTTGTTATCATATCA+TGG + chr4.3:39426055-39426074 MS.gene004011:intron 25.0%
!!! CATTTCTTTGGAACTAATAA+TGG + chr4.3:39426687-39426706 MS.gene004011:CDS 25.0%
!!! CTGGAGTTTATACTTTTTTA+AGG + chr4.3:39425267-39425286 MS.gene004011:intron 25.0%
!!! TGGTATTTTATATTTCTAGC+TGG + chr4.3:39425248-39425267 MS.gene004011:intron 25.0%
!!! TTACTAACCAAAGTATTGTT+TGG - chr4.3:39426771-39426790 None:intergenic 25.0%
AAAATAGTTCTTACCAATGC+AGG - chr4.3:39424642-39424661 None:intergenic 30.0%
ACTTATTTAAGCTGATCTGT+TGG - chr4.3:39427299-39427318 None:intergenic 30.0%
CAACAAGATTAAACTTTGCA+GGG - chr4.3:39426950-39426969 None:intergenic 30.0%
GACAGATACATACAAATTGA+AGG + chr4.3:39426077-39426096 MS.gene004011:intron 30.0%
TAGTTCCAAAGAAATGTTGT+TGG - chr4.3:39426683-39426702 None:intergenic 30.0%
TATGAATGTATGGTTGAAAC+AGG + chr4.3:39427445-39427464 MS.gene004011:intron 30.0%
TCAACAAGATTAAACTTTGC+AGG - chr4.3:39426951-39426970 None:intergenic 30.0%
TCATATGTACCAAAGAGAAT+GGG - chr4.3:39425181-39425200 None:intergenic 30.0%
TGAGAAAATAGGATTTGAAC+TGG + chr4.3:39424736-39424755 MS.gene004011:intron 30.0%
TGCATGCATGATAAAAAAAC+AGG + chr4.3:39424924-39424943 MS.gene004011:intron 30.0%
TTAAATCCATGAAGATGCAA+AGG - chr4.3:39426879-39426898 None:intergenic 30.0%
TTCATATGTACCAAAGAGAA+TGG - chr4.3:39425182-39425201 None:intergenic 30.0%
! AAATTAGTATCATTGATGGC+TGG - chr4.3:39426441-39426460 None:intergenic 30.0%
! CGTAATAGATATGTTTTTCC+TGG - chr4.3:39426108-39426127 None:intergenic 30.0%
! TGTGAGTTTTGCAGTTAATT+AGG - chr4.3:39424704-39424723 None:intergenic 30.0%
!! AATTTCTTTGTTGTTGGTCA+AGG + chr4.3:39426897-39426916 MS.gene004011:CDS 30.0%
!! ATTATTTCTGCAGATAAGGT+TGG + chr4.3:39424483-39424502 MS.gene004011:intron 30.0%
!!! CTTGACAAGTTTGGTTTTTT+GGG - chr4.3:39426581-39426600 None:intergenic 30.0%
AACTCCTCCAAACAATACTT+TGG + chr4.3:39426761-39426780 MS.gene004011:CDS 35.0%
AACTCTGACCTGTTTGAATT+GGG - chr4.3:39425009-39425028 None:intergenic 35.0%
AAGTAGCCAAAACAAATCCA+GGG - chr4.3:39424290-39424309 None:intergenic 35.0%
AATGTGGCATTTGGGTAATT+TGG - chr4.3:39426288-39426307 None:intergenic 35.0%
ACAACGACATTTGATATGCT+TGG - chr4.3:39424765-39424784 None:intergenic 35.0%
ACCATATAACACTAGAGTTC+AGG + chr4.3:39426812-39426831 MS.gene004011:CDS 35.0%
ACCTGAACTCTAGTGTTATA+TGG - chr4.3:39426816-39426835 None:intergenic 35.0%
CAACGACATTTGATATGCTT+GGG - chr4.3:39424764-39424783 None:intergenic 35.0%
CATATGTACCAAAGAGAATG+GGG - chr4.3:39425180-39425199 None:intergenic 35.0%
CCAAAAAACCAAACTTGTCA+AGG + chr4.3:39426579-39426598 MS.gene004011:CDS 35.0%
CTTCTCCAACAACATTTCTT+TGG + chr4.3:39426675-39426694 MS.gene004011:CDS 35.0%
GAAAAAACCAAGTTCCACTT+AGG + chr4.3:39425895-39425914 MS.gene004011:intron 35.0%
GATAAGATTCATTGTGCCTT+GGG - chr4.3:39425132-39425151 None:intergenic 35.0%
GCAAAAAGAGCCTTTGATAA+TGG - chr4.3:39424231-39424250 None:intergenic 35.0%
GCATGATAAAAAAACAGGCA+TGG + chr4.3:39424929-39424948 MS.gene004011:intron 35.0%
GGAGTTCCTGTATAGTTAAA+CGG - chr4.3:39426747-39426766 None:intergenic 35.0%
GTATAGTTAAACGGCCTTAA+CGG - chr4.3:39426738-39426757 None:intergenic 35.0%
TAACTCTGACCTGTTTGAAT+TGG - chr4.3:39425010-39425029 None:intergenic 35.0%
TAGCTAGACCATATTTCACA+GGG + chr4.3:39426316-39426335 MS.gene004011:CDS 35.0%
TAGTAATGGTACAAAGACAG+TGG + chr4.3:39426785-39426804 MS.gene004011:CDS 35.0%
TATTTGGTCCTTGACAAGTT+TGG - chr4.3:39426590-39426609 None:intergenic 35.0%
TCTTTATCCTAAGTGGAACT+TGG - chr4.3:39425905-39425924 None:intergenic 35.0%
TGTACATCATGGATCTAGTT+AGG + chr4.3:39424848-39424867 MS.gene004011:intron 35.0%
TGTAGGCATCAGAAACATTT+GGG - chr4.3:39425452-39425471 None:intergenic 35.0%
TGTCACTGTTCCATTATCAA+AGG + chr4.3:39424218-39424237 MS.gene004011:CDS 35.0%
TTAAGGCCGTTTAACTATAC+AGG + chr4.3:39426738-39426757 MS.gene004011:CDS 35.0%
! ATTGCAGGATACAAGCATTT+TGG + chr4.3:39426839-39426858 MS.gene004011:CDS 35.0%
! TAGAGTTCAGGTTATATTGC+AGG + chr4.3:39426824-39426843 MS.gene004011:CDS 35.0%
! TCCTTTTCAAAAACCCTACA+AGG + chr4.3:39425150-39425169 MS.gene004011:CDS 35.0%
! TGATTGACTATGGTTCTGTT+TGG + chr4.3:39427046-39427065 MS.gene004011:intron 35.0%
! TTGCAGGATACAAGCATTTT+GGG + chr4.3:39426840-39426859 MS.gene004011:CDS 35.0%
! TTTTTCTTTACCGTTGGACT+AGG + chr4.3:39426543-39426562 MS.gene004011:CDS 35.0%
!! CTAACCAAAGTATTGTTTGG+AGG - chr4.3:39426768-39426787 None:intergenic 35.0%
!! CTTATTTTCGTAAGTTCTCC+AGG - chr4.3:39427204-39427223 None:intergenic 35.0%
!! TCCTTGTAGGGTTTTTGAAA+AGG - chr4.3:39425154-39425173 None:intergenic 35.0%
!! TGGTTTTACAACGGATTTTC+CGG + chr4.3:39426707-39426726 MS.gene004011:CDS 35.0%
!! TTCTAAAATTCCAGCTACTG+TGG - chr4.3:39426361-39426380 None:intergenic 35.0%
!!! ATAGTTTTAGGCACATTTGC+AGG - chr4.3:39426513-39426532 None:intergenic 35.0%
!!! CCTTGACAAGTTTGGTTTTT+TGG - chr4.3:39426582-39426601 None:intergenic 35.0%
!!! GAAGCACAGGTTTTAGAATA+TGG - chr4.3:39426419-39426438 None:intergenic 35.0%
!!! TAGTTTTAGGCACATTTGCA+GGG - chr4.3:39426512-39426531 None:intergenic 35.0%
!!! TTTGTTGTTGGTCAAGGTTT+TGG + chr4.3:39426903-39426922 MS.gene004011:CDS 35.0%
AAAGCATGGTTACCGTGAAT+GGG + chr4.3:39424533-39424552 MS.gene004011:CDS 40.0%
AAATATGCATAGGTGAGTGG+TGG + chr4.3:39425376-39425395 MS.gene004011:intron 40.0%
AACTAAGGTGCACATCAAAG+TGG - chr4.3:39427489-39427508 None:intergenic 40.0%
AATACCATCATACTTGGACC+AGG + chr4.3:39426231-39426250 MS.gene004011:CDS 40.0%
ACAATCCAGGTGATTGACTA+TGG + chr4.3:39427036-39427055 MS.gene004011:intron 40.0%
ACGTGCAGCTTGTATATAAC+TGG + chr4.3:39425518-39425537 MS.gene004011:intron 40.0%
ACTCAATGCCCAATTCAAAC+AGG + chr4.3:39424998-39425017 MS.gene004011:CDS 40.0%
ACTTTGATGTGCACCTTAGT+TGG + chr4.3:39427488-39427507 MS.gene004011:CDS 40.0%
AGAACATTTGTGGTTTGACC+TGG - chr4.3:39426252-39426271 None:intergenic 40.0%
AGCATGTAGAATGTGGCATT+TGG - chr4.3:39426297-39426316 None:intergenic 40.0%
AGGAAACCCATTAATGGTGT+AGG - chr4.3:39425469-39425488 None:intergenic 40.0%
AGTAGCCAAAACAAATCCAG+GGG - chr4.3:39424289-39424308 None:intergenic 40.0%
AGTCATCCTTTGCATCTTCA+TGG + chr4.3:39426870-39426889 MS.gene004011:CDS 40.0%
AGTTCCTCTTTGTCCAACAA+TGG - chr4.3:39425047-39425066 None:intergenic 40.0%
ATCTTGTTGATCCTGTTGAG+AGG + chr4.3:39426961-39426980 MS.gene004011:CDS 40.0%
CAAACAATATCAGCCTGCAT+TGG + chr4.3:39424626-39424645 MS.gene004011:CDS 40.0%
CAATGCAGGCTGATATTGTT+TGG - chr4.3:39424628-39424647 None:intergenic 40.0%
CCTCTTTACAATTGCTCCAA+AGG + chr4.3:39425492-39425511 MS.gene004011:CDS 40.0%
CTAGCTAGACCATATTTCAC+AGG + chr4.3:39426315-39426334 MS.gene004011:CDS 40.0%
CTCGAATTGTTGTACGAGAA+GGG + chr4.3:39424569-39424588 MS.gene004011:CDS 40.0%
CTGATGCCTACACCATTAAT+GGG + chr4.3:39425460-39425479 MS.gene004011:CDS 40.0%
CTTTGATGTGCACCTTAGTT+GGG + chr4.3:39427489-39427508 MS.gene004011:CDS 40.0%
GAAGTAGCCAAAACAAATCC+AGG - chr4.3:39424291-39424310 None:intergenic 40.0%
GACAAATATGCATAGGTGAG+TGG + chr4.3:39425373-39425392 MS.gene004011:intron 40.0%
GAGTCCAATACCATCATACT+TGG + chr4.3:39426225-39426244 MS.gene004011:CDS 40.0%
GCATGTAGAATGTGGCATTT+GGG - chr4.3:39426296-39426315 None:intergenic 40.0%
GGATAAGATTCATTGTGCCT+TGG - chr4.3:39425133-39425152 None:intergenic 40.0%
GTATGGTTGAAACAGGTGTT+TGG + chr4.3:39427452-39427471 MS.gene004011:intron 40.0%
GTGTACAACTTCACCATTGT+TGG + chr4.3:39425031-39425050 MS.gene004011:CDS 40.0%
GTGTAGGCATCAGAAACATT+TGG - chr4.3:39425453-39425472 None:intergenic 40.0%
GTGTGTGAGAGTAAAACTCA+TGG - chr4.3:39424796-39424815 None:intergenic 40.0%
TACAAATTGAAGGTGAAGCC+AGG + chr4.3:39426087-39426106 MS.gene004011:CDS 40.0%
TCGAATTGTTGTACGAGAAG+GGG + chr4.3:39424570-39424589 MS.gene004011:CDS 40.0%
TCTAGCTAGCATGTAGAATG+TGG - chr4.3:39426304-39426323 None:intergenic 40.0%
TCTGATGCCTACACCATTAA+TGG + chr4.3:39425459-39425478 MS.gene004011:CDS 40.0%
TGGTCCAAGTATGATGGTAT+TGG - chr4.3:39426232-39426251 None:intergenic 40.0%
TGGTTAAGAGCTACTGTCTA+TGG + chr4.3:39425091-39425110 MS.gene004011:CDS 40.0%
TTCACCATTGTTGGACAAAG+AGG + chr4.3:39425040-39425059 MS.gene004011:CDS 40.0%
TTTGATGTGCACCTTAGTTG+GGG + chr4.3:39427490-39427509 MS.gene004011:CDS 40.0%
! AAAAGCATGGTTACCGTGAA+TGG + chr4.3:39424532-39424551 MS.gene004011:CDS 40.0%
! AGGGGACAGATTATTGGTTA+AGG + chr4.3:39424588-39424607 MS.gene004011:CDS 40.0%
! ATGATGGTATTGGACTCGAA+TGG - chr4.3:39426222-39426241 None:intergenic 40.0%
! ATTTTCCGGTTGTACCGTTA+AGG + chr4.3:39426721-39426740 MS.gene004011:CDS 40.0%
!! CCTTTGGAGCAATTGTAAAG+AGG - chr4.3:39425495-39425514 None:intergenic 40.0%
!! GGAGGAGGCAATTTTTGATT+AGG - chr4.3:39427553-39427572 None:intergenic 40.0%
!! GGGCATTGAGTTATGTATGA+TGG - chr4.3:39424989-39425008 None:intergenic 40.0%
!! TACACCATTAATGGGTTTCC+TGG + chr4.3:39425468-39425487 MS.gene004011:CDS 40.0%
!! TGTTGGAGAAGTGCTATAGA+AGG - chr4.3:39426666-39426685 None:intergenic 40.0%
!!! CAAGTTTGGTTTTTTGGGCA+TGG - chr4.3:39426576-39426595 None:intergenic 40.0%
!!! GTTTTGGCTACTTCACAACA+TGG + chr4.3:39424297-39424316 MS.gene004011:CDS 40.0%
AAAATTGCCTCCTCCTCCTA+AGG + chr4.3:39427558-39427577 MS.gene004011:CDS 45.0%
AAGATGCATCTGCTTCGACA+AGG - chr4.3:39426191-39426210 None:intergenic 45.0%
AAGGAAGTCCCCATTCTCTT+TGG + chr4.3:39425169-39425188 MS.gene004011:CDS 45.0%
AATACTGTTGCTGTGCCTTC+TGG + chr4.3:39426984-39427003 MS.gene004011:CDS 45.0%
AATCACCTGGATTGTCAGCA+AGG - chr4.3:39427031-39427050 None:intergenic 45.0%
ACAACTTCGTAGTGGTTGGT+CGG + chr4.3:39424958-39424977 MS.gene004011:CDS 45.0%
ACGAGAAGGGGACAGATTAT+TGG + chr4.3:39424582-39424601 MS.gene004011:CDS 45.0%
AGAATGGGGACTTCCTTGTA+GGG - chr4.3:39425166-39425185 None:intergenic 45.0%
AGACCATATTTCACAGGGCA+GGG + chr4.3:39426321-39426340 MS.gene004011:CDS 45.0%
AGGTCCAGGAAACCCATTAA+TGG - chr4.3:39425475-39425494 None:intergenic 45.0%
ATTCATTGTGCCTTGGGAGA+AGG - chr4.3:39425126-39425145 None:intergenic 45.0%
CAACATTTGGGGAGATCCTT+AGG - chr4.3:39427577-39427596 None:intergenic 45.0%
CAGAACCATAGTCAATCACC+TGG - chr4.3:39427044-39427063 None:intergenic 45.0%
CCTCGAATTGTTGTACGAGA+AGG + chr4.3:39424568-39424587 MS.gene004011:CDS 45.0%
CCTTCTCGTACAACAATTCG+AGG - chr4.3:39424571-39424590 None:intergenic 45.0%
GACAAAGAGGAACTCTCTTC+TGG + chr4.3:39425053-39425072 MS.gene004011:CDS 45.0%
GAGCAATTGTAAAGAGGTCC+AGG - chr4.3:39425489-39425508 None:intergenic 45.0%
GCAACAGTATTCCTCTCAAC+AGG - chr4.3:39426975-39426994 None:intergenic 45.0%
GCTTGTGCAATTACAGCTTC+AGG - chr4.3:39425411-39425430 None:intergenic 45.0%
TAAGGTTGGCAAAGGTTACG+AGG + chr4.3:39424497-39424516 MS.gene004011:CDS 45.0%
TAGACCATATTTCACAGGGC+AGG + chr4.3:39426320-39426339 MS.gene004011:CDS 45.0%
TCTGCAGATAAGGTTGGCAA+AGG + chr4.3:39424489-39424508 MS.gene004011:intron 45.0%
TCTGGCATGCTCATTTCTCA+TGG + chr4.3:39425071-39425090 MS.gene004011:CDS 45.0%
TGAGACAACTTCGTAGTGGT+TGG + chr4.3:39424954-39424973 MS.gene004011:CDS 45.0%
TTCGACAATTCCACAGTAGC+TGG + chr4.3:39426348-39426367 MS.gene004011:CDS 45.0%
TTGACCTGGTCCAAGTATGA+TGG - chr4.3:39426238-39426257 None:intergenic 45.0%
! ATGGCTTGGATTGTTGAGGA+TGG + chr4.3:39427520-39427539 MS.gene004011:CDS 45.0%
!! AACCTCCCCTGGATTTGTTT+TGG + chr4.3:39424281-39424300 MS.gene004011:CDS 45.0%
!! CGGTAACCATGCTTTTTGTG+TGG - chr4.3:39424528-39424547 None:intergenic 45.0%
!! GGCACATTTGCAGGGTATTT+TGG - chr4.3:39426504-39426523 None:intergenic 45.0%
!! TGGACTAGTTCCTAGTCCAA+CGG - chr4.3:39426556-39426575 None:intergenic 45.0%
!! TAAAATATTATTAATTAAAG+TGG - chr4.3:39425860-39425879 None:intergenic 5.0%
AACGGCCTTAACGGTACAAC+CGG - chr4.3:39426729-39426748 None:intergenic 50.0%
ACAAGCTGCACGTGTACCTT+TGG - chr4.3:39425511-39425530 None:intergenic 50.0%
AGCCAAAACAAATCCAGGGG+AGG - chr4.3:39424286-39424305 None:intergenic 50.0%
ATTGCACAAGCTCTGCAGAC+AGG + chr4.3:39425420-39425439 MS.gene004011:CDS 50.0%
CAAATCCAGGGGAGGTTAAG+AGG - chr4.3:39424278-39424297 None:intergenic 50.0%
CATTTGGGGAGATCCTTAGG+AGG - chr4.3:39427574-39427593 None:intergenic 50.0%
CCATCCTCAATCCCCAACTA+AGG - chr4.3:39427504-39427523 None:intergenic 50.0%
CCTTAGTTGGGGATTGAGGA+TGG + chr4.3:39427501-39427520 MS.gene004011:CDS 50.0%
CGATTCCTTGCTGACAATCC+AGG + chr4.3:39427023-39427042 MS.gene004011:CDS 50.0%
CGTACAACAATTCGAGGACC+CGG - chr4.3:39424565-39424584 None:intergenic 50.0%
GAGAATGGGGACTTCCTTGT+AGG - chr4.3:39425167-39425186 None:intergenic 50.0%
GCTGTGCCACACAAAAAGCA+TGG + chr4.3:39424519-39424538 MS.gene004011:CDS 50.0%
GGTGTGAGACAACTTCGTAG+TGG + chr4.3:39424950-39424969 MS.gene004011:CDS 50.0%
GTACAACAATTCGAGGACCC+GGG - chr4.3:39424564-39424583 None:intergenic 50.0%
GTACCCTGCCCTGTGAAATA+TGG - chr4.3:39426327-39426346 None:intergenic 50.0%
TACCGTGAATGGGAAGTTCC+CGG + chr4.3:39424543-39424562 MS.gene004011:CDS 50.0%
TGCACCTTAGTTGGGGATTG+AGG + chr4.3:39427497-39427516 MS.gene004011:CDS 50.0%
TTGCACAAGCTCTGCAGACA+GGG + chr4.3:39425421-39425440 MS.gene004011:CDS 50.0%
! GAGGATGGCTTGGATTGTTG+AGG + chr4.3:39427516-39427535 MS.gene004011:CDS 50.0%
! TCATTGATGGCTGGAAGCAC+AGG - chr4.3:39426432-39426451 None:intergenic 50.0%
ACCCGGGAACTTCCCATTCA+CGG - chr4.3:39424548-39424567 None:intergenic 55.0%
ACCGTGAATGGGAAGTTCCC+GGG + chr4.3:39424544-39424563 MS.gene004011:CDS 55.0%
ACCTTCCTCTTAACCTCCCC+TGG + chr4.3:39424270-39424289 MS.gene004011:CDS 55.0%
ACTGTTGCTGTGCCTTCTGG+TGG + chr4.3:39426987-39427006 MS.gene004011:CDS 55.0%
AGGGGAGGTTAAGAGGAAGG+TGG - chr4.3:39424271-39424290 None:intergenic 55.0%
GACCTCTCATCCTTCTCCCA+AGG + chr4.3:39425113-39425132 MS.gene004011:CDS 55.0%
GTTGGGGATTGAGGATGGCT+TGG + chr4.3:39427506-39427525 MS.gene004011:CDS 55.0%
TCCAGGGGAGGTTAAGAGGA+AGG - chr4.3:39424274-39424293 None:intergenic 55.0%
TGCACAAGCTCTGCAGACAG+GGG + chr4.3:39425422-39425441 MS.gene004011:CDS 55.0%
TGCCTTGGGAGAAGGATGAG+AGG - chr4.3:39425118-39425137 None:intergenic 55.0%
TGCTGTGCCTTCTGGTGGTT+GGG + chr4.3:39426992-39427011 MS.gene004011:CDS 55.0%
TTGCTGTGCCTTCTGGTGGT+TGG + chr4.3:39426991-39427010 MS.gene004011:CDS 55.0%
TTGGGGAGATCCTTAGGAGG+AGG - chr4.3:39427571-39427590 None:intergenic 55.0%
! CTTCGTAGTGGTTGGTCGGA+TGG + chr4.3:39424962-39424981 MS.gene004011:CDS 55.0%
ATCGCGACCCAACCACCAGA+AGG - chr4.3:39427002-39427021 None:intergenic 60.0%
GCACAAGCTCTGCAGACAGG+GGG + chr4.3:39425423-39425442 MS.gene004011:CDS 60.0%
GGGAGATCCTTAGGAGGAGG+AGG - chr4.3:39427568-39427587 None:intergenic 60.0%
CAAGCTCTGCAGACAGGGGG+TGG + chr4.3:39425426-39425445 MS.gene004011:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 39424201 39427597 39424201 ID=MS.gene004011
chr4.3 mRNA 39424201 39427597 39424201 ID=MS.gene004011.t1;Parent=MS.gene004011
chr4.3 exon 39424201 39424347 39424201 ID=MS.gene004011.t1.exon1;Parent=MS.gene004011.t1
chr4.3 CDS 39424201 39424347 39424201 ID=cds.MS.gene004011.t1;Parent=MS.gene004011.t1
chr4.3 exon 39424496 39424647 39424496 ID=MS.gene004011.t1.exon2;Parent=MS.gene004011.t1
chr4.3 CDS 39424496 39424647 39424496 ID=cds.MS.gene004011.t1;Parent=MS.gene004011.t1
chr4.3 exon 39424946 39425190 39424946 ID=MS.gene004011.t1.exon3;Parent=MS.gene004011.t1
chr4.3 CDS 39424946 39425190 39424946 ID=cds.MS.gene004011.t1;Parent=MS.gene004011.t1
chr4.3 exon 39425388 39425513 39425388 ID=MS.gene004011.t1.exon4;Parent=MS.gene004011.t1
chr4.3 CDS 39425388 39425513 39425388 ID=cds.MS.gene004011.t1;Parent=MS.gene004011.t1
chr4.3 exon 39426082 39427044 39426082 ID=MS.gene004011.t1.exon5;Parent=MS.gene004011.t1
chr4.3 CDS 39426082 39427044 39426082 ID=cds.MS.gene004011.t1;Parent=MS.gene004011.t1
chr4.3 exon 39427467 39427597 39427467 ID=MS.gene004011.t1.exon6;Parent=MS.gene004011.t1
chr4.3 CDS 39427467 39427597 39427467 ID=cds.MS.gene004011.t1;Parent=MS.gene004011.t1
Gene Sequence

>MS.gene004011

ATGCATATCAAAATGATTGTCACTGTTCCATTATCAAAGGCTCTTTTTGCAGCTTTTCTCTTTGTCACCACCTTCCTCTTAACCTCCCCTGGATTTGTTTTGGCTACTTCACAACATGGAAGTGTTACAAGACACTACAAATTTGATGTATATACACTTTATATTATATAAATTAATTCCTTTCAAATATTTATCACTTAATTAATTAACTTAAAACCTACCAAATTGTATAACATATGTAGTGTAAGAAAAAAAAAAGTTTTATAGTATATCATTGTTTTTATTATTTCTGCAGATAAGGTTGGCAAAGGTTACGAGGCTGTGCCACACAAAAAGCATGGTTACCGTGAATGGGAAGTTCCCGGGTCCTCGAATTGTTGTACGAGAAGGGGACAGATTATTGGTTAAGGTTGTTAATCATGTTCCAAACAATATCAGCCTGCATTGGTAAGAACTATTTTTTTTAATTATTTGTAATTTTTCACATTTTTTTCAAAGAGCCTAATTAACTGCAAAACTCACAAAAACTAAGTGTTGAGAAAATAGGATTTGAACTGGCGCCCAAGCATATCAAATGTCGTTGTAGTTTTCACCATGAGTTTTACTCTCACACACACGCAATTTGTTTTTACATTTATATTGTATGTTGTACATCATGGATCTAGTTAGGATATCCATATGCATGAAATGAATTATTGTACTAATATTGACAATAATGGTGAATGCATGCATGATAAAAAAACAGGCATGGTGTGAGACAACTTCGTAGTGGTTGGTCGGATGGTCCATCATACATAACTCAATGCCCAATTCAAACAGGTCAGAGTTATGTGTACAACTTCACCATTGTTGGACAAAGAGGAACTCTCTTCTGGCATGCTCATTTCTCATGGTTAAGAGCTACTGTCTATGGACCTCTCATCCTTCTCCCAAGGCACAATGAATCTTATCCTTTTCAAAAACCCTACAAGGAAGTCCCCATTCTCTTTGGTACATATGAACAATCATTCCTCAGTATAAATTTATAATAAATAATTTATTATACTCTGGTATTTTATATTTCTAGCTGGAGTTTATACTTTTTTAAGGAAAGATATAATAAAGATATTTTTTTTATTTTACAAAAAAATAGTATATGATATGAAAGGTTTTGTAATCTTATACATATCTTTGACAAATATGCATAGGTGAGTGGTGGAATGCAGATCCTGAAGCTGTAATTGCACAAGCTCTGCAGACAGGGGGTGGCCCAAATGTTTCTGATGCCTACACCATTAATGGGTTTCCTGGACCTCTTTACAATTGCTCCAAAGGTACACGTGCAGCTTGTATATAACTGGCATTTCTAGTTATTTGTTTTTAATTAAGAACTAAATAAATTTTGTACATTTAATGTAATTTTTTTTACACTAATATATATATATATATATATATATATATATATATATATATATATATATATATATTAACATAACAAAGTGATATGATACTAAAATGTGATTTAATTGAATTTATGTTTAAAAAAGTTCTACATAGTTAAAGAGTTTTTTTTTCTTTCTCAATTCATTAAATAGAAGTAAATTGATTATATGTTGTTTATTTAGCTTAATTTCGTTTATAAATAACACAATATCTTACAAGTTGCTTTGCTTAACACCACTTTAATTAATAATATTTTATTTTATTTTATTAGAGAAAAAACCAAGTTCCACTTAGGATAAAGAAAATGTTTGAAATGAGTTTATAAATAGTGACACCTCTCATTTTACAAACTAATTTGTAAAAATCGAGTTAGATAAATTGACGAATAATGACTAGAATTGATTGGTTTTTTTTTTTTTGAAAGATGAATTGATTGGTTTGTTATCATATCATGGACAGATACATACAAATTGAAGGTGAAGCCAGGAAAAACATATCTATTACGTTTGATCAACGCTGCACTCAACGACGAACTCTTTTTCAGCATTGCTAATCACACATTAATCCTTGTCGAAGCAGATGCATCTTACATTAAACCATTCGAGTCCAATACCATCATACTTGGACCAGGTCAAACCACAAATGTTCTATTAAAAACTAAACCAAATTACCCAAATGCCACATTCTACATGCTAGCTAGACCATATTTCACAGGGCAGGGTACTTTCGACAATTCCACAGTAGCTGGAATTTTAGAATACACTAAACCAAATGATCAAACAAATAAAAATCTCCATATTCTAAAACCTGTGCTTCCAGCCATCAATGATACTAATTTTGTTGCTAATTTTAGTAACAAATTTCTTAGCTTAAATAGTCCAAAATACCCTGCAAATGTGCCTAAAACTATTGACAAAAACTTTTTCTTTACCGTTGGACTAGGAACTAGTCCATGCCCAAAAAACCAAACTTGTCAAGGACCAAATAATAGTTCAAATTCAAAATTTGCAGCTTCAATGAACAATGTATCATTTGCTTTACCTTCTATAGCACTTCTCCAACAACATTTCTTTGGAACTAATAATGGTTTTACAACGGATTTTCCGGTTGTACCGTTAAGGCCGTTTAACTATACAGGAACTCCTCCAAACAATACTTTGGTTAGTAATGGTACAAAGACAGTGGTGATACCATATAACACTAGAGTTCAGGTTATATTGCAGGATACAAGCATTTTGGGAGCTGAGAGTCATCCTTTGCATCTTCATGGATTTAATTTCTTTGTTGTTGGTCAAGGTTTTGGAAATTTTAATGCTAGTAGTGACCCTGCAAAGTTTAATCTTGTTGATCCTGTTGAGAGGAATACTGTTGCTGTGCCTTCTGGTGGTTGGGTCGCGATACGATTCCTTGCTGACAATCCAGGTGATTGACTATGGTTCTGTTTGGATAATCAGCTTAATTAAGCACTTATATCACAAGCACTTGTCATATAAGCGTTTATGTATAGCTATTTCTATAAAAAATGACAAAAAAGTCAAACTGTTTTTATATAAGCTATAAACTATTTTCATAAACTATCCTGGAGAACTTACGAAAATAAGTTAAAAACAATGTCGTAAGTTGTTTTCAATTTATTTTATAAGCTCTTCTAAACAGTCTTACAAGTGTTTATGCCAACAGATCAGCTTAAATAAGTCAATCCAAATATTATATATCATATAGTTTTTCTTATCTTGTATCTCAAGTTATTTATATGATACTTTGATCACTTTAATTATAAAGCATATAATGAATTAATTAGACTTTTTTTTGATCTATTTGCTATGAATGTATGGTTGAAACAGGTGTTTGGCTGATGCATTGCCACTTTGATGTGCACCTTAGTTGGGGATTGAGGATGGCTTGGATTGTTGAGGATGGAAAGTTACCTAATCAAAAATTGCCTCCTCCTCCTAAGGATCTCCCCAAATGTTGA
Protein sequence

>MS.gene004011.t1

MHIKMIVTVPLSKALFAAFLFVTTFLLTSPGFVLATSQHGSVTRHYKFDIRLAKVTRLCHTKSMVTVNGKFPGPRIVVREGDRLLVKVVNHVPNNISLHWHGVRQLRSGWSDGPSYITQCPIQTGQSYVYNFTIVGQRGTLFWHAHFSWLRATVYGPLILLPRHNESYPFQKPYKEVPILFGEWWNADPEAVIAQALQTGGGPNVSDAYTINGFPGPLYNCSKDTYKLKVKPGKTYLLRLINAALNDELFFSIANHTLILVEADASYIKPFESNTIILGPGQTTNVLLKTKPNYPNATFYMLARPYFTGQGTFDNSTVAGILEYTKPNDQTNKNLHILKPVLPAINDTNFVANFSNKFLSLNSPKYPANVPKTIDKNFFFTVGLGTSPCPKNQTCQGPNNSSNSKFAASMNNVSFALPSIALLQQHFFGTNNGFTTDFPVVPLRPFNYTGTPPNNTLVSNGTKTVVIPYNTRVQVILQDTSILGAESHPLHLHGFNFFVVGQGFGNFNASSDPAKFNLVDPVERNTVAVPSGGWVAIRFLADNPGVWLMHCHFDVHLSWGLRMAWIVEDGKLPNQKLPPPPKDLPKC