AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70354

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70354.t1 MTR_8g091390 98.440 577 8 1 1 576 1 577 0.0 1180
MS.gene70354.t1 MTR_3g078740 48.522 575 258 11 31 573 23 591 0.0 551
MS.gene70354.t1 MTR_3g078730 49.209 569 250 9 13 573 5 542 0.0 550
MS.gene70354.t1 MTR_3g078700 47.544 570 261 7 6 569 1 538 0.0 542
MS.gene70354.t1 MTR_5g020600 34.024 579 325 19 1 552 1 549 1.96e-85 276
MS.gene70354.t1 MTR_5g020620 33.628 565 325 14 7 549 3 539 2.15e-83 271
MS.gene70354.t1 MTR_5g081810 34.549 521 294 15 49 549 14 507 5.00e-79 259
MS.gene70354.t1 MTR_5g069680 33.635 553 324 14 14 549 10 536 1.03e-78 259
MS.gene70354.t1 MTR_7g458880 31.615 582 337 20 19 564 14 570 8.99e-78 257
MS.gene70354.t1 MTR_5g083360 31.810 591 334 20 15 566 7 567 8.65e-77 254
MS.gene70354.t1 MTR_3g462760 33.457 541 311 16 30 549 22 534 1.24e-76 253
MS.gene70354.t1 MTR_7g058690 32.107 598 335 23 4 561 1 567 1.59e-75 251
MS.gene70354.t1 MTR_7g062250 32.387 599 338 23 7 566 8 578 2.76e-75 250
MS.gene70354.t1 MTR_7g060460 32.186 581 334 20 18 562 13 569 3.80e-75 250
MS.gene70354.t1 MTR_2g008330 32.241 580 327 18 7 549 7 557 8.18e-74 246
MS.gene70354.t1 MTR_7g062310 32.927 574 314 22 28 560 41 584 1.99e-71 240
MS.gene70354.t1 MTR_8g027375 31.397 551 331 15 22 549 23 549 4.18e-70 236
MS.gene70354.t1 MTR_5g073210 31.716 577 334 20 17 563 5 551 1.04e-69 235
MS.gene70354.t1 MTR_4g064530 30.369 596 349 19 1 560 6 571 2.34e-68 232
MS.gene70354.t1 MTR_3g101640 31.524 571 323 20 15 549 11 549 7.26e-68 230
MS.gene70354.t1 MTR_7g065980 29.776 581 347 17 18 565 10 562 1.13e-66 227
MS.gene70354.t1 MTR_7g065970 29.791 574 338 19 15 550 3 549 2.17e-66 226
MS.gene70354.t1 MTR_3g101635 31.327 565 319 19 21 549 13 544 1.93e-65 224
MS.gene70354.t1 MTR_3g071890 32.150 535 289 17 49 546 48 545 6.35e-65 222
MS.gene70354.t1 MTR_4g019225 31.540 539 304 20 49 550 41 551 1.02e-64 222
MS.gene70354.t1 MTR_4g064530 29.772 571 338 18 1 535 6 549 5.06e-61 211
MS.gene70354.t1 MTR_8g479390 28.295 569 328 20 28 571 25 538 6.27e-55 194
MS.gene70354.t1 MTR_5g018270 28.142 565 331 16 28 571 25 535 9.36e-53 189
MS.gene70354.t1 MTR_4g132320 27.444 583 343 15 15 571 11 539 6.38e-52 186
MS.gene70354.t1 MTR_3g008580 27.444 583 359 17 17 570 8 555 8.55e-52 187
MS.gene70354.t1 MTR_4g049390 28.219 567 320 16 34 571 27 535 1.26e-51 186
MS.gene70354.t1 MTR_4g048350 27.739 566 333 15 29 571 22 534 9.00e-51 183
MS.gene70354.t1 MTR_4g101650 29.472 587 352 19 8 568 1 551 1.14e-50 184
MS.gene70354.t1 MTR_3g115050 26.094 594 346 18 10 571 6 538 7.33e-50 181
MS.gene70354.t1 MTR_3g083440 28.082 584 351 18 9 570 3 539 2.64e-49 179
MS.gene70354.t1 MTR_2g088080 26.678 596 347 15 8 571 1 538 7.15e-49 178
MS.gene70354.t1 MTR_3g083400 28.082 584 351 18 9 570 3 539 7.34e-49 178
MS.gene70354.t1 MTR_1g026410 26.633 597 341 18 11 571 2 537 7.65e-49 178
MS.gene70354.t1 MTR_3g083390 27.911 584 352 17 9 570 3 539 1.26e-48 177
MS.gene70354.t1 MTR_3g083450 28.315 558 334 17 35 570 27 540 5.64e-48 176
MS.gene70354.t1 MTR_3g083430 27.740 584 353 17 9 570 3 539 6.72e-48 176
MS.gene70354.t1 MTR_1g090707 27.593 540 325 19 52 568 42 538 1.19e-47 176
MS.gene70354.t1 MTR_4g075690 26.034 580 352 16 14 571 13 537 7.05e-47 172
MS.gene70354.t1 MTR_7g051440 29.159 559 331 20 35 567 30 549 1.98e-46 172
MS.gene70354.t1 MTR_4g083210 28.130 583 348 19 9 570 3 535 4.38e-46 170
MS.gene70354.t1 MTR_8g479380 26.460 582 343 19 15 570 13 535 2.24e-45 168
MS.gene70354.t1 MTR_3g083410 27.881 538 322 17 55 570 10 503 4.51e-45 167
MS.gene70354.t1 MTR_4g083190 26.562 576 364 16 14 570 6 541 6.45e-44 164
MS.gene70354.t1 MTR_4g075690 25.767 489 292 13 14 485 13 447 9.43e-38 145
MS.gene70354.t1 MTR_2g088080 25.681 514 296 13 87 571 1 457 3.12e-37 144
MS.gene70354.t1 MTR_4g015120 41.615 161 90 2 389 549 379 535 2.67e-32 131
MS.gene70354.t1 MTR_3g079660 50.000 96 47 1 481 575 5 100 8.68e-30 113
MS.gene70354.t1 MTR_5g081790 35.981 214 121 5 338 541 225 432 7.95e-26 110
MS.gene70354.t1 MTR_5g081790 31.970 269 152 11 12 256 3 264 1.50e-22 100
MS.gene70354.t1 MTR_2g009230 44.262 61 32 1 139 197 1 61 2.88e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70354.t1 AT4G39830 65.901 566 184 4 14 570 16 581 0.0 813
MS.gene70354.t1 AT4G39830 67.355 484 149 4 96 570 3 486 0.0 699
MS.gene70354.t1 AT4G39830 67.288 483 149 4 97 570 2 484 0.0 697
MS.gene70354.t1 AT4G39830 65.083 484 147 5 96 570 3 473 0.0 663
MS.gene70354.t1 AT5G21105 49.911 563 269 7 20 573 24 582 0.0 567
MS.gene70354.t1 AT5G21100 48.381 556 275 7 26 573 16 567 0.0 551
MS.gene70354.t1 AT5G21105 48.282 524 258 7 15 529 19 538 6.34e-173 501
MS.gene70354.t1 AT5G21105 48.698 384 186 6 197 573 12 391 5.41e-120 360
MS.gene70354.t1 AT5G03260 35.189 557 314 15 14 549 5 535 5.60e-88 283
MS.gene70354.t1 AT5G58910 32.979 564 330 16 12 549 4 545 3.47e-82 268
MS.gene70354.t1 AT5G58910 32.979 564 329 17 12 549 4 544 3.99e-81 265
MS.gene70354.t1 AT2G38080 33.273 553 328 13 17 549 5 536 1.17e-80 264
MS.gene70354.t1 AT1G18140 31.359 574 334 17 27 564 22 571 6.50e-77 254
MS.gene70354.t1 AT5G05390 32.162 569 321 20 14 549 7 543 1.88e-76 253
MS.gene70354.t1 AT5G01050 30.862 580 331 21 20 561 13 560 7.03e-74 246
MS.gene70354.t1 AT5G09360 33.042 572 324 19 19 564 21 559 1.29e-73 245
MS.gene70354.t1 AT5G60020 31.404 570 336 17 11 545 2 551 5.96e-73 244
MS.gene70354.t1 AT2G29130 31.095 566 330 18 14 545 8 547 1.18e-72 243
MS.gene70354.t1 AT5G58910 32.887 523 300 16 49 549 8 501 1.33e-71 239
MS.gene70354.t1 AT2G46570 30.810 568 331 18 13 549 11 547 2.82e-71 239
MS.gene70354.t1 AT5G48100 32.470 579 325 26 16 561 5 550 1.77e-69 234
MS.gene70354.t1 AT5G01190 33.274 559 319 15 16 549 7 536 1.39e-68 232
MS.gene70354.t1 AT5G60020 31.767 532 308 17 49 545 8 519 2.30e-68 231
MS.gene70354.t1 AT5G01040 30.623 578 333 21 20 561 13 558 4.19e-68 231
MS.gene70354.t1 AT3G09220 30.728 563 332 20 18 549 10 545 6.09e-68 230
MS.gene70354.t1 AT5G60020 30.866 554 328 17 11 529 2 535 1.08e-65 224
MS.gene70354.t1 AT5G07130 30.298 571 336 18 12 549 6 547 5.93e-65 223
MS.gene70354.t1 AT5G01190 34.165 521 289 15 54 549 31 522 5.97e-65 222
MS.gene70354.t1 AT2G40370 29.897 582 331 20 14 549 8 558 5.02e-64 220
MS.gene70354.t1 AT2G30210 30.366 573 333 20 13 549 6 548 2.60e-63 218
MS.gene70354.t1 AT1G75790 29.010 586 338 20 15 570 8 545 1.04e-57 202
MS.gene70354.t1 AT4G12420 30.797 552 326 19 13 544 5 520 8.46e-56 198
MS.gene70354.t1 AT4G12420 30.797 552 326 19 13 544 5 520 8.46e-56 198
MS.gene70354.t1 AT5G66920 27.305 564 339 15 28 571 31 543 9.31e-55 194
MS.gene70354.t1 AT2G23630 28.938 584 321 21 18 570 16 536 1.36e-53 191
MS.gene70354.t1 AT2G23630 28.938 584 321 21 18 570 21 541 1.40e-53 191
MS.gene70354.t1 AT4G22010 27.807 579 342 17 17 571 9 535 1.94e-53 191
MS.gene70354.t1 AT2G46570 30.061 489 281 17 92 549 4 462 5.14e-53 188
MS.gene70354.t1 AT1G21850 27.711 581 340 20 15 571 11 535 1.67e-51 186
MS.gene70354.t1 AT1G55570 28.049 574 354 14 17 571 12 545 2.79e-51 185
MS.gene70354.t1 AT4G37160 27.534 592 347 19 5 570 3 538 1.43e-50 183
MS.gene70354.t1 AT1G76160 26.486 589 345 18 10 571 8 535 4.36e-50 181
MS.gene70354.t1 AT3G09220 30.021 473 274 18 108 549 2 448 1.57e-48 176
MS.gene70354.t1 AT1G21860 27.007 548 326 16 16 544 11 503 1.82e-48 177
MS.gene70354.t1 AT4G28090 28.154 547 311 18 23 544 18 507 1.99e-48 177
MS.gene70354.t1 AT5G07130 28.770 504 299 16 100 566 4 484 1.01e-47 174
MS.gene70354.t1 AT5G07130 28.770 504 299 16 100 566 4 484 1.01e-47 174
MS.gene70354.t1 AT5G48450 27.667 553 345 15 8 544 1 514 6.22e-46 171
MS.gene70354.t1 AT5G48450 27.437 554 345 15 8 544 1 514 1.67e-45 170
MS.gene70354.t1 AT1G41830 24.538 595 353 19 9 571 6 536 4.06e-45 167
MS.gene70354.t1 AT3G13390 27.046 562 339 16 35 571 29 544 8.63e-45 167
MS.gene70354.t1 AT4G25240 27.883 581 357 18 15 570 11 554 2.48e-44 166
MS.gene70354.t1 AT4G25240 27.883 581 357 18 15 570 11 554 2.48e-44 166
MS.gene70354.t1 AT5G51480 26.690 562 352 18 36 572 30 556 4.65e-43 163
MS.gene70354.t1 AT3G13400 26.401 553 355 15 35 572 28 543 2.45e-41 157
MS.gene70354.t1 AT3G13400 26.401 553 355 15 35 572 28 543 2.45e-41 157
MS.gene70354.t1 AT4G38420 24.957 581 359 19 16 569 12 542 8.79e-39 150
MS.gene70354.t1 AT1G55560 26.512 562 341 18 36 572 28 542 8.65e-36 141
MS.gene70354.t1 AT4G37160 27.092 502 290 17 5 485 3 449 1.42e-35 139
MS.gene70354.t1 AT4G38420 25.287 522 318 18 16 515 12 483 1.63e-33 134
MS.gene70354.t1 AT4G38420 25.407 492 296 17 16 485 12 454 1.43e-30 125

Find 138 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTATGTTTCTTTCTAATTC+TGG 0.152360 4.2:-10537761 MS.gene70354:CDS
AAACTTGTTATAACCATTAA+TGG 0.170163 4.2:-10537655 MS.gene70354:CDS
TGAAATTGCTTGTTTAATTA+TGG 0.174792 4.2:+10534943 None:intergenic
AATGAATGATTACCTCTATT+TGG 0.179597 4.2:+10536501 None:intergenic
CCTCAGTCACTTCTGATTCA+TGG 0.203458 4.2:-10536680 MS.gene70354:intron
CAAAGGGAAGCAGGAGTTTA+TGG 0.209011 4.2:-10536965 MS.gene70354:CDS
ACTGTGCCAGTACACTTATT+TGG 0.264792 4.2:-10535137 MS.gene70354:CDS
CTTCTAGCAATGGAATTTAT+AGG 0.273291 4.2:-10535346 MS.gene70354:CDS
GGCACAGTATTCTTCATAAT+GGG 0.274165 4.2:+10535152 None:intergenic
GGCGACCTCATTGGATGGTT+TGG 0.294439 4.2:+10535692 None:intergenic
TTTATAGATTTGTTGTTGAT+AGG 0.309393 4.2:-10537311 MS.gene70354:CDS
GAGGTTTGATTGTGTAATTT+TGG 0.312038 4.2:+10535591 None:intergenic
CTGGTGTTAGACATTATAAA+TGG 0.319055 4.2:-10537717 MS.gene70354:CDS
TATAGAGTCTCATTTCTATA+TGG 0.319868 4.2:-10535057 MS.gene70354:CDS
ACAAGTTTCTTATAGCAATC+AGG 0.322015 4.2:+10537670 None:intergenic
CTATCAAAGTCATAAGAAAA+AGG 0.331498 4.2:+10536911 None:intergenic
ATTGGAACTCCTTGGTTTGA+TGG 0.340104 4.2:-10537381 MS.gene70354:CDS
GGTGGAGGTGGTGGAGATGT+TGG 0.347451 4.2:+10535668 None:intergenic
TGCGAAAAGGGAGAGCATTC+AGG 0.359438 4.2:+10536593 None:intergenic
GCGACCTCATTGGATGGTTT+GGG 0.364061 4.2:+10535693 None:intergenic
GGCAAAAGCAAAGGCTTCTT+AGG 0.366890 4.2:-10534966 MS.gene70354:CDS
GAAGCATTATCTTGAATGAT+TGG 0.371573 4.2:-10536889 MS.gene70354:CDS
AGCCATCAGGTGGAGGTGTT+TGG 0.375855 4.2:+10535420 None:intergenic
CTTTAAGTGCAACAAGATAA+GGG 0.376250 4.2:+10535465 None:intergenic
TATCTTTATCATGCTCACTA+TGG 0.377739 4.2:-10536992 MS.gene70354:CDS
TATTTGGTTGGACTGCTTTG+AGG 0.387190 4.2:-10535121 MS.gene70354:CDS
GTGATCCAGTAGTTTCTTGA+TGG 0.389887 4.2:+10535776 None:intergenic
TGGCACAGTATTCTTCATAA+TGG 0.391850 4.2:+10535151 None:intergenic
GGGAGGTGAAGTATGATTAT+AGG 0.405545 4.2:-10537696 MS.gene70354:CDS
ATCACCTTCTTGTGCTTGAA+TGG 0.406079 4.2:+10537615 None:intergenic
TCTTTAAGTGCAACAAGATA+AGG 0.407309 4.2:+10535464 None:intergenic
GCACTTAAAGAGAATATAAC+TGG 0.408038 4.2:-10535452 MS.gene70354:CDS
ATTGTTCATAAGTACTCTTA+TGG 0.412906 4.2:+10536864 None:intergenic
AAGAAGGAATTGAAAGGGTT+GGG 0.416848 4.2:-10535016 MS.gene70354:CDS
CCTTCTTGTGCTTGAATGGT+AGG 0.418298 4.2:+10537619 None:intergenic
GAGACGACTGTTTGCGAAAA+GGG 0.423092 4.2:+10536581 None:intergenic
TGCAAGGCTTTGAGTATGCC+TGG 0.424280 4.2:+10535625 None:intergenic
TAACACCAGCCTCAGCTTTG+TGG 0.425338 4.2:+10537731 None:intergenic
GTTTCTGCTAACAACATTTG+AGG 0.431005 4.2:+10535754 None:intergenic
ACACATCCATGGCATCTTCA+TGG 0.435000 4.2:-10535254 MS.gene70354:CDS
TCCCATGCTGGTGGAGGTGG+TGG 0.442090 4.2:+10535659 None:intergenic
ATAGAGTCTCATTTCTATAT+GGG 0.445190 4.2:-10535056 MS.gene70354:CDS
TAGAGATTCTTAACCACAAA+AGG 0.447645 4.2:+10535848 None:intergenic
AGAACAACCCCACCTGGTTT+AGG 0.454476 4.2:-10535731 MS.gene70354:CDS
ACTCTATCTGAGGTTGTTGG+AGG 0.454902 4.2:+10535572 None:intergenic
CAAGTTTCTTATAGCAATCA+GGG 0.467175 4.2:+10537671 None:intergenic
GAAGCTACCTTCATCCATCA+TGG 0.470885 4.2:-10534994 MS.gene70354:CDS
TTATAGATTTGTTGTTGATA+GGG 0.471713 4.2:-10537310 MS.gene70354:intron
TTTCTATATGGGAATGGGAG+TGG 0.472301 4.2:-10535045 MS.gene70354:CDS
AGCACTCAGTTTCCAAATAG+AGG 0.474284 4.2:-10536513 MS.gene70354:intron
TTGTCCCATGCTGGTGGAGG+TGG 0.475191 4.2:+10535656 None:intergenic
TGGGTACTTGGATATGGAAA+GGG 0.475912 4.2:-10535221 MS.gene70354:CDS
TCACTTCTGATTCATGGAAA+AGG 0.477199 4.2:-10536674 MS.gene70354:CDS
TGTGCTTGAATGGTAGGTCC+TGG 0.483708 4.2:+10537625 None:intergenic
GAAGAAGGAATTGAAAGGGT+TGG 0.486528 4.2:-10535017 MS.gene70354:CDS
TATGACAGATTGGAACTCCT+TGG 0.490012 4.2:-10537389 MS.gene70354:intron
TCTCATTTCTATATGGGAAT+GGG 0.493176 4.2:-10535050 MS.gene70354:CDS
GTCTCATTTCTATATGGGAA+TGG 0.494997 4.2:-10535051 MS.gene70354:CDS
TGAGACGACTGTTTGCGAAA+AGG 0.496730 4.2:+10536580 None:intergenic
TATGGAATGCAAAGGGAAGC+AGG 0.500152 4.2:-10536974 MS.gene70354:CDS
ATGACTCTATCTGAGGTTGT+TGG 0.501248 4.2:+10535569 None:intergenic
TTCAACTTCAACTAAGCCTA+AGG 0.502063 4.2:-10537804 MS.gene70354:CDS
TGCCAGTACACTTATTTGGT+TGG 0.503206 4.2:-10535133 MS.gene70354:CDS
GACTCAACATTGTCCCATGC+TGG 0.505407 4.2:+10535647 None:intergenic
AGTACTTATGAACAATCTGC+TGG 0.505703 4.2:-10536857 MS.gene70354:CDS
CTATAGACAATGTTCGTCAT+TGG 0.506850 4.2:-10535523 MS.gene70354:CDS
TTTGGTGAGCTTTGATTGCA+AGG 0.510484 4.2:+10535609 None:intergenic
GGAGGTGGTGGAGATGTTGG+TGG 0.512910 4.2:+10535671 None:intergenic
TTGGGTACTTGGATATGGAA+AGG 0.514165 4.2:-10535222 MS.gene70354:CDS
CAGAAAACCTTGCCATCCAT+TGG 0.515007 4.2:-10537558 MS.gene70354:CDS
AGGTTTAGATCAGATAATCC+TGG 0.515420 4.2:-10535101 MS.gene70354:CDS
TGATCCAGTAGTTTCTTGAT+GGG 0.518958 4.2:+10535777 None:intergenic
CCATTGGCATGGTATTAGAC+AGG 0.519566 4.2:-10537542 MS.gene70354:intron
GATGTTGGTGGCGACCTCAT+TGG 0.527521 4.2:+10535683 None:intergenic
CAAGATAAGGGGTGTGAGGT+AGG 0.528066 4.2:+10535477 None:intergenic
CTATAAACAAAGGTGTCTCC+AGG 0.530116 4.2:+10537327 None:intergenic
TTATGAAAATCATAGCCATC+AGG 0.531401 4.2:+10535407 None:intergenic
GTCCAACCAAATAAGTGTAC+TGG 0.532587 4.2:+10535131 None:intergenic
TTATTACCAAGCTTACCTGC+TGG 0.533199 4.2:-10536635 MS.gene70354:CDS
CCTTCATCCATCATGGGTTG+TGG 0.536511 4.2:-10534987 MS.gene70354:CDS
TGCTCACTATGGAATGCAAA+GGG 0.536716 4.2:-10536981 MS.gene70354:CDS
CCTTGGTTTGATGGAACAGA+AGG 0.537286 4.2:-10537372 MS.gene70354:CDS
AAGACCCATCAAGAAACTAC+TGG 0.542638 4.2:-10535781 MS.gene70354:CDS
AATTTCCACAAAGCTGAGGC+TGG 0.543418 4.2:-10537736 MS.gene70354:CDS
TACCATGCCAATGGATGGCA+AGG 0.543974 4.2:+10537551 None:intergenic
TGGTGTTAGACATTATAAAT+GGG 0.546733 4.2:-10537716 MS.gene70354:CDS
CAAACACCAGCAGGTAAGCT+TGG 0.547162 4.2:+10536629 None:intergenic
ACAAATGCTACTTCTAGCAA+TGG 0.556255 4.2:-10535356 MS.gene70354:CDS
CTCCACCACCTCCACCAGCA+TGG 0.556607 4.2:-10535661 MS.gene70354:CDS
TCCACCACCTCCACCAGCAT+GGG 0.556708 4.2:-10535660 MS.gene70354:CDS
CATGGGACAATGTTGAGTCC+AGG 0.557068 4.2:-10535643 MS.gene70354:CDS
ATCAGATAATCCTGGTGTGT+GGG 0.557217 4.2:-10535093 MS.gene70354:CDS
CAATGGAAAGCCCACACACC+AGG 0.564141 4.2:+10535083 None:intergenic
CCACAACCCATGATGGATGA+AGG 0.564653 4.2:+10534987 None:intergenic
GATGAGTTACAAACACCAGC+AGG 0.565048 4.2:+10536620 None:intergenic
ACATTGTCCCATGCTGGTGG+AGG 0.567912 4.2:+10535653 None:intergenic
TTGGTGGCGACCTCATTGGA+TGG 0.568529 4.2:+10535687 None:intergenic
ATGGGTTGTGGCAAAAGCAA+AGG 0.574548 4.2:-10534975 MS.gene70354:CDS
ATGCTCACTATGGAATGCAA+AGG 0.577390 4.2:-10536982 MS.gene70354:CDS
TCATGTCCATGAAGATGCCA+TGG 0.579018 4.2:+10535248 None:intergenic
TCTAATACCATGCCAATGGA+TGG 0.581741 4.2:+10537546 None:intergenic
TCTCAATGTCCTATACTACC+TGG 0.584728 4.2:-10537345 MS.gene70354:CDS
GATCAGATAATCCTGGTGTG+TGG 0.587744 4.2:-10535094 MS.gene70354:CDS
ATGACTGTTGTTGAAGCAGA+TGG 0.588116 4.2:-10535884 MS.gene70354:CDS
AAGGTGTCTCCAGGTAGTAT+AGG 0.588687 4.2:+10537336 None:intergenic
TCAACATTGTCCCATGCTGG+TGG 0.589698 4.2:+10535650 None:intergenic
AGAAACAGAACAACCCCACC+TGG 0.592580 4.2:-10535737 MS.gene70354:CDS
AAGCTACCTTCATCCATCAT+GGG 0.594823 4.2:-10534993 MS.gene70354:CDS
AACCTTGCCATCCATTGGCA+TGG 0.596188 4.2:-10537553 MS.gene70354:CDS
AAATCATAGCCATCAGGTGG+AGG 0.597280 4.2:+10535413 None:intergenic
CCTGTCTAATACCATGCCAA+TGG 0.599234 4.2:+10537542 None:intergenic
AAGAATCTCTACATATACTC+AGG 0.600439 4.2:-10535836 MS.gene70354:CDS
AACCAAACACCTCCACCTGA+TGG 0.600941 4.2:-10535422 MS.gene70354:CDS
CTTCAACAACAGTCATGTTG+TGG 0.606047 4.2:+10535891 None:intergenic
GAAAATGCCTAAACCAGGTG+GGG 0.606749 4.2:+10535724 None:intergenic
TGAAAATGCCTAAACCAGGT+GGG 0.607601 4.2:+10535723 None:intergenic
CCTACCATTCAAGCACAAGA+AGG 0.608130 4.2:-10537619 MS.gene70354:CDS
CTTGGTTTGATGGAACAGAA+GGG 0.611684 4.2:-10537371 MS.gene70354:CDS
AATGAGACTCTATATGACAA+TGG 0.612162 4.2:+10535066 None:intergenic
CAAAACTATGACTCTATCTG+AGG 0.612834 4.2:+10535562 None:intergenic
TTGAAAATGCCTAAACCAGG+TGG 0.618052 4.2:+10535722 None:intergenic
GGTGAATTTCCACAAAGCTG+AGG 0.618690 4.2:-10537740 MS.gene70354:CDS
TTGGTTTGATGGAACAGAAG+GGG 0.622382 4.2:-10537370 MS.gene70354:CDS
TTTAAGTGCAACAAGATAAG+GGG 0.625406 4.2:+10535466 None:intergenic
TGAAAATCATAGCCATCAGG+TGG 0.630112 4.2:+10535410 None:intergenic
AACAACAAATCTATAAACAA+AGG 0.630677 4.2:+10537317 None:intergenic
ATAATAGTGAGACACATCCA+TGG 0.633125 4.2:-10535265 MS.gene70354:CDS
TCAACTTCAACTAAGCCTAA+GGG 0.636069 4.2:-10537803 MS.gene70354:CDS
GCACAGTATTCTTCATAATG+GGG 0.640153 4.2:+10535153 None:intergenic
GCATGATAAAGATATGTACC+AGG 0.647073 4.2:+10537001 None:intergenic
GCAACAAGATAAGGGGTGTG+AGG 0.654315 4.2:+10535473 None:intergenic
AAGTTTCTTATAGCAATCAG+GGG 0.665367 4.2:+10537672 None:intergenic
TAGTTGAAAATGCCTAAACC+AGG 0.675427 4.2:+10535719 None:intergenic
TGTTAGACATTATAAATGGG+AGG 0.685230 4.2:-10537713 MS.gene70354:CDS
AGTTTATGGAATGATTCGTG+TGG 0.691203 4.2:-10536951 MS.gene70354:CDS
CCTTCTGTTCCATCAAACCA+AGG 0.696461 4.2:+10537372 None:intergenic
ACTACCCAAACCATCCAATG+AGG 0.700116 4.2:-10535697 MS.gene70354:CDS
GCTGAAGTTCAATACAACTG+TGG 0.701274 4.2:-10535324 MS.gene70354:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAATCTATTCATT+TGG - chr4.2:10535302-10535321 MS.gene70354:CDS 10.0%
!!! CAAAATATTTTAAATTTAAC+AGG - chr4.2:10535261-10535280 MS.gene70354:CDS 10.0%
!!! CTGTTAAATTTAAAATATTT+TGG + chr4.2:10535263-10535282 None:intergenic 10.0%
!!! AAACATAGAAACAAAAATTT+TGG + chr4.2:10534992-10535011 None:intergenic 15.0%
!!! TTATTGTTTCTTTGTATATT+TGG - chr4.2:10536057-10536076 MS.gene70354:intron 15.0%
!!! TTATTTATTTCTTAGTCATA+TGG - chr4.2:10536571-10536590 MS.gene70354:CDS 15.0%
!! AACAACAAATCTATAAACAA+AGG + chr4.2:10535450-10535469 None:intergenic 20.0%
!! ATATGAGTTTGAAGAAATTA+TGG - chr4.2:10535526-10535545 MS.gene70354:CDS 20.0%
!! TTAATATATAATGACTGATG+TGG - chr4.2:10535346-10535365 MS.gene70354:CDS 20.0%
!!! AAAATATTTTGGACTAGTAA+AGG + chr4.2:10535252-10535271 None:intergenic 20.0%
!!! AAACTTGTTATAACCATTAA+TGG - chr4.2:10535109-10535128 MS.gene70354:CDS 20.0%
!!! TCACCAAATTGTAATTTTTT+GGG + chr4.2:10537590-10537609 None:intergenic 20.0%
!!! TTATAGATTTGTTGTTGATA+GGG - chr4.2:10535454-10535473 MS.gene70354:CDS 20.0%
!!! TTCACCAAATTGTAATTTTT+TGG + chr4.2:10537591-10537610 None:intergenic 20.0%
!!! TTTATAGATTTGTTGTTGAT+AGG - chr4.2:10535453-10535472 MS.gene70354:CDS 20.0%
! AAGCGAGAAAAATTAAACAA+AGG + chr4.2:10536809-10536828 None:intergenic 25.0%
! AATCATTAAGCACCAAATAT+AGG + chr4.2:10535328-10535347 None:intergenic 25.0%
! AATGAATGATTACCTCTATT+TGG + chr4.2:10536266-10536285 None:intergenic 25.0%
! AGACCCAAAAAATTACAATT+TGG - chr4.2:10537584-10537603 MS.gene70354:CDS 25.0%
! ATAGAGTCTCATTTCTATAT+GGG - chr4.2:10537708-10537727 MS.gene70354:CDS 25.0%
! ATATGGTTGAACTACAATTT+AGG - chr4.2:10536588-10536607 MS.gene70354:CDS 25.0%
! ATTGTTCATAAGTACTCTTA+TGG + chr4.2:10535903-10535922 None:intergenic 25.0%
! CTATCAAAGTCATAAGAAAA+AGG + chr4.2:10535856-10535875 None:intergenic 25.0%
! TATAGAGTCTCATTTCTATA+TGG - chr4.2:10537707-10537726 MS.gene70354:CDS 25.0%
! TATGGTTGAACTACAATTTA+GGG - chr4.2:10536589-10536608 MS.gene70354:CDS 25.0%
! TCTATGTTTCTTTCTAATTC+TGG - chr4.2:10535003-10535022 MS.gene70354:CDS 25.0%
! TTAAAGCCTCAATAAAATCA+TGG + chr4.2:10535557-10535576 None:intergenic 25.0%
! TTCTTCAATTGTCTAATTGT+TGG - chr4.2:10536032-10536051 MS.gene70354:intron 25.0%
!! ATCATCCAAGTTTTTAAACA+AGG - chr4.2:10536304-10536323 MS.gene70354:intron 25.0%
!! CATGATTCAATTGGTTTAAT+CGG + chr4.2:10536633-10536652 None:intergenic 25.0%
!! TAATTACGATATTTTCAGTG+TGG - chr4.2:10537377-10537396 MS.gene70354:CDS 25.0%
!! TGGTGTTAGACATTATAAAT+GGG - chr4.2:10535048-10535067 MS.gene70354:CDS 25.0%
!!! TTTTGAAGAAGGAATTGAAA+GGG - chr4.2:10537743-10537762 MS.gene70354:CDS 25.0%
!!! TTTTTGAAGAAGGAATTGAA+AGG - chr4.2:10537742-10537761 MS.gene70354:CDS 25.0%
AACCAGATGTAAGAATTAGA+TGG - chr4.2:10535962-10535981 MS.gene70354:intron 30.0%
AAGAATCTCTACATATACTC+AGG - chr4.2:10536928-10536947 MS.gene70354:CDS 30.0%
AAGTCATAAGAAAAAGGTTC+AGG + chr4.2:10535850-10535869 None:intergenic 30.0%
AAGTTTCTTATAGCAATCAG+GGG + chr4.2:10535095-10535114 None:intergenic 30.0%
AATGAGACTCTATATGACAA+TGG + chr4.2:10537701-10537720 None:intergenic 30.0%
ACAAGTTTCTTATAGCAATC+AGG + chr4.2:10535097-10535116 None:intergenic 30.0%
ACATATTTAATGTTACAGCC+TGG - chr4.2:10535745-10535764 MS.gene70354:CDS 30.0%
ATCCATCTAATTCTTACATC+TGG + chr4.2:10535967-10535986 None:intergenic 30.0%
CAAGTTTCTTATAGCAATCA+GGG + chr4.2:10535096-10535115 None:intergenic 30.0%
CTATTCATTTGGCCTATATT+TGG - chr4.2:10535313-10535332 MS.gene70354:CDS 30.0%
CTTCTAGCAATGGAATTTAT+AGG - chr4.2:10537418-10537437 MS.gene70354:intron 30.0%
CTTTAAGTGCAACAAGATAA+GGG + chr4.2:10537302-10537321 None:intergenic 30.0%
GCACTTAAAGAGAATATAAC+TGG - chr4.2:10537312-10537331 MS.gene70354:CDS 30.0%
GTGTTGAAATTGAAGAACAA+CGG + chr4.2:10535665-10535684 None:intergenic 30.0%
TAGAGATTCTTAACCACAAA+AGG + chr4.2:10536919-10536938 None:intergenic 30.0%
TATCTTTATCATGCTCACTA+TGG - chr4.2:10535772-10535791 MS.gene70354:CDS 30.0%
TCTCATTTCTATATGGGAAT+GGG - chr4.2:10537714-10537733 MS.gene70354:CDS 30.0%
TCTTTAAGTGCAACAAGATA+AGG + chr4.2:10537303-10537322 None:intergenic 30.0%
TGAGTTTGAAGAAATTATGG+TGG - chr4.2:10535529-10535548 MS.gene70354:CDS 30.0%
TGTTAGACATTATAAATGGG+AGG - chr4.2:10535051-10535070 MS.gene70354:CDS 30.0%
TTATGAAAATCATAGCCATC+AGG + chr4.2:10537360-10537379 None:intergenic 30.0%
TTTAAGTGCAACAAGATAAG+GGG + chr4.2:10537301-10537320 None:intergenic 30.0%
TTTGTATAAAGAAGACAGAC+TGG + chr4.2:10536522-10536541 None:intergenic 30.0%
! ATCAAACCAGTAAGACTTTT+AGG + chr4.2:10536657-10536676 None:intergenic 30.0%
! ATCTTCATGGACATGATTTT+TGG - chr4.2:10537523-10537542 MS.gene70354:intron 30.0%
! CTGGTGTTAGACATTATAAA+TGG - chr4.2:10535047-10535066 MS.gene70354:CDS 30.0%
! GAACTACAATTTAGGGTTAA+GGG - chr4.2:10536596-10536615 MS.gene70354:CDS 30.0%
! GAAGCATTATCTTGAATGAT+TGG - chr4.2:10535875-10535894 MS.gene70354:CDS 30.0%
! GAGGTTTGATTGTGTAATTT+TGG + chr4.2:10537176-10537195 None:intergenic 30.0%
! TCTTCATGGACATGATTTTT+GGG - chr4.2:10537524-10537543 MS.gene70354:intron 30.0%
! TGAACTACAATTTAGGGTTA+AGG - chr4.2:10536595-10536614 MS.gene70354:CDS 30.0%
!! CTTTTAGGTCATGATTCAAT+TGG + chr4.2:10536642-10536661 None:intergenic 30.0%
!!! CTGATTCTATCGGTTTTATA+GGG + chr4.2:10536467-10536486 None:intergenic 30.0%
AAGAAGGAATTGAAAGGGTT+GGG - chr4.2:10537748-10537767 MS.gene70354:CDS 35.0%
ACACGACAATGTTGTGAAAT+CGG - chr4.2:10536372-10536391 MS.gene70354:intron 35.0%
ACCATTAATGGAAAAACTCC+AGG - chr4.2:10535121-10535140 MS.gene70354:CDS 35.0%
AGGTTTAGATCAGATAATCC+TGG - chr4.2:10537663-10537682 MS.gene70354:CDS 35.0%
AGTACTTATGAACAATCTGC+TGG - chr4.2:10535907-10535926 MS.gene70354:intron 35.0%
AGTTTATGGAATGATTCGTG+TGG - chr4.2:10535813-10535832 MS.gene70354:CDS 35.0%
ATAATAGTGAGACACATCCA+TGG - chr4.2:10537499-10537518 MS.gene70354:intron 35.0%
CAAAACTATGACTCTATCTG+AGG + chr4.2:10537205-10537224 None:intergenic 35.0%
CATTGTTGTCAAAACAAGAC+CGG + chr4.2:10536682-10536701 None:intergenic 35.0%
CCTATAAAACCGATAGAATC+AGG - chr4.2:10536465-10536484 MS.gene70354:intron 35.0%
CTATAGACAATGTTCGTCAT+TGG - chr4.2:10537241-10537260 MS.gene70354:intron 35.0%
GACATGTTTGTAATGATGCA+GGG - chr4.2:10536850-10536869 MS.gene70354:CDS 35.0%
GCACAGTATTCTTCATAATG+GGG + chr4.2:10537614-10537633 None:intergenic 35.0%
GCATGATAAAGATATGTACC+AGG + chr4.2:10535766-10535785 None:intergenic 35.0%
GGCACAGTATTCTTCATAAT+GGG + chr4.2:10537615-10537634 None:intergenic 35.0%
GTCTCATTTCTATATGGGAA+TGG - chr4.2:10537713-10537732 MS.gene70354:CDS 35.0%
GTTTCTGCTAACAACATTTG+AGG + chr4.2:10537013-10537032 None:intergenic 35.0%
TAGTTGAAAATGCCTAAACC+AGG + chr4.2:10537048-10537067 None:intergenic 35.0%
TCAACTTCAACTAAGCCTAA+GGG - chr4.2:10534961-10534980 MS.gene70354:CDS 35.0%
TCACTTCTGATTCATGGAAA+AGG - chr4.2:10536090-10536109 MS.gene70354:intron 35.0%
TCATGACCTAAAAGTCTTAC+TGG - chr4.2:10536648-10536667 MS.gene70354:CDS 35.0%
TCGCAAAATGACTACGATTA+AGG - chr4.2:10536710-10536729 MS.gene70354:intron 35.0%
TGACATGTTTGTAATGATGC+AGG - chr4.2:10536849-10536868 MS.gene70354:CDS 35.0%
TGATCCAGTAGTTTCTTGAT+GGG + chr4.2:10536990-10537009 None:intergenic 35.0%
TGGCACAGTATTCTTCATAA+TGG + chr4.2:10537616-10537635 None:intergenic 35.0%
TTCAACTTCAACTAAGCCTA+AGG - chr4.2:10534960-10534979 MS.gene70354:CDS 35.0%
! ACAAATGCTACTTCTAGCAA+TGG - chr4.2:10537408-10537427 MS.gene70354:intron 35.0%
! ACACTATGTTGATCCTTTTG+TGG - chr4.2:10536903-10536922 MS.gene70354:CDS 35.0%
! GAAAAAGGTTCAGGTTCATT+AGG + chr4.2:10535841-10535860 None:intergenic 35.0%
! GCAGACCTTGTTTAAAAACT+TGG + chr4.2:10536312-10536331 None:intergenic 35.0%
! GTGGTTTTTGTATGACAGAT+TGG - chr4.2:10535365-10535384 MS.gene70354:CDS 35.0%
! TCCTGGAGTTTTTCCATTAA+TGG + chr4.2:10535125-10535144 None:intergenic 35.0%
! TGGTTTTCAGTTAAACCAGT+CGG + chr4.2:10536399-10536418 None:intergenic 35.0%
! TTGATGATCAACTGTGAGTT+TGG + chr4.2:10535623-10535642 None:intergenic 35.0%
!!! CCTGATTCTATCGGTTTTAT+AGG + chr4.2:10536468-10536487 None:intergenic 35.0%
!!! GATTTTTGGGTACTTGGATA+TGG - chr4.2:10537537-10537556 MS.gene70354:intron 35.0%
AACTGCGGTTCAACAAGAAT+TGG - chr4.2:10536491-10536510 MS.gene70354:intron 40.0%
AAGACCCATCAAGAAACTAC+TGG - chr4.2:10536983-10537002 MS.gene70354:CDS 40.0%
AAGCTACCTTCATCCATCAT+GGG - chr4.2:10537771-10537790 MS.gene70354:CDS 40.0%
ACTGTGCCAGTACACTTATT+TGG - chr4.2:10537627-10537646 MS.gene70354:CDS 40.0%
AGCACTCAGTTTCCAAATAG+AGG - chr4.2:10536251-10536270 MS.gene70354:intron 40.0%
ATCACCTTCTTGTGCTTGAA+TGG + chr4.2:10535152-10535171 None:intergenic 40.0%
ATCAGATAATCCTGGTGTGT+GGG - chr4.2:10537671-10537690 MS.gene70354:CDS 40.0%
ATGACTGTTGTTGAAGCAGA+TGG - chr4.2:10536880-10536899 MS.gene70354:CDS 40.0%
ATGCAGTTGTGGTAATAGTC+TGG + chr4.2:10536781-10536800 None:intergenic 40.0%
ATGCTCACTATGGAATGCAA+AGG - chr4.2:10535782-10535801 MS.gene70354:CDS 40.0%
CAAAGGCTAAAATGCAGTTG+TGG + chr4.2:10536792-10536811 None:intergenic 40.0%
CATGAATCAGAAGTGACTGA+GGG + chr4.2:10536086-10536105 None:intergenic 40.0%
CGTGTGTACATCAAAAACGA+TGG + chr4.2:10536357-10536376 None:intergenic 40.0%
CTGTGAGTTTGGCAAAATCA+TGG + chr4.2:10535612-10535631 None:intergenic 40.0%
CTTCAACAACAGTCATGTTG+TGG + chr4.2:10536876-10536895 None:intergenic 40.0%
GAAGAAGGAATTGAAAGGGT+TGG - chr4.2:10537747-10537766 MS.gene70354:CDS 40.0%
GCAGTTCAACCTGATTCTAT+CGG + chr4.2:10536477-10536496 None:intergenic 40.0%
GCTGAAGTTCAATACAACTG+TGG - chr4.2:10537440-10537459 MS.gene70354:intron 40.0%
GGGAGGTGAAGTATGATTAT+AGG - chr4.2:10535068-10535087 MS.gene70354:CDS 40.0%
GTCCAACCAAATAAGTGTAC+TGG + chr4.2:10537636-10537655 None:intergenic 40.0%
GTGATCCAGTAGTTTCTTGA+TGG + chr4.2:10536991-10537010 None:intergenic 40.0%
TATGACAGATTGGAACTCCT+TGG - chr4.2:10535375-10535394 MS.gene70354:CDS 40.0%
TATTTGGTTGGACTGCTTTG+AGG - chr4.2:10537643-10537662 MS.gene70354:CDS 40.0%
TCTAATACCATGCCAATGGA+TGG + chr4.2:10535221-10535240 None:intergenic 40.0%
TCTCAATGTCCTATACTACC+TGG - chr4.2:10535419-10535438 MS.gene70354:CDS 40.0%
TGAAAATCATAGCCATCAGG+TGG + chr4.2:10537357-10537376 None:intergenic 40.0%
TGAAAATGCCTAAACCAGGT+GGG + chr4.2:10537044-10537063 None:intergenic 40.0%
TGCCAGTACACTTATTTGGT+TGG - chr4.2:10537631-10537650 MS.gene70354:CDS 40.0%
TGCTCACTATGGAATGCAAA+GGG - chr4.2:10535783-10535802 MS.gene70354:CDS 40.0%
TGGGTACTTGGATATGGAAA+GGG - chr4.2:10537543-10537562 MS.gene70354:CDS 40.0%
TTATTACCAAGCTTACCTGC+TGG - chr4.2:10536129-10536148 MS.gene70354:intron 40.0%
TTGAAAATGCCTAAACCAGG+TGG + chr4.2:10537045-10537064 None:intergenic 40.0%
TTGGGTACTTGGATATGGAA+AGG - chr4.2:10537542-10537561 MS.gene70354:intron 40.0%
TTTCTATATGGGAATGGGAG+TGG - chr4.2:10537719-10537738 MS.gene70354:CDS 40.0%
! ATGACTCTATCTGAGGTTGT+TGG + chr4.2:10537198-10537217 None:intergenic 40.0%
! ATTGGAACTCCTTGGTTTGA+TGG - chr4.2:10535383-10535402 MS.gene70354:CDS 40.0%
! CTTGGTTTGATGGAACAGAA+GGG - chr4.2:10535393-10535412 MS.gene70354:CDS 40.0%
! GTCTGCAATCCCTTTTCAAT+GGG - chr4.2:10535933-10535952 MS.gene70354:intron 40.0%
! GTGGCACCATGATTTTATTG+AGG - chr4.2:10535548-10535567 MS.gene70354:CDS 40.0%
! TGTCTGCAATCCCTTTTCAA+TGG - chr4.2:10535932-10535951 MS.gene70354:intron 40.0%
! TTGGTTTGATGGAACAGAAG+GGG - chr4.2:10535394-10535413 MS.gene70354:CDS 40.0%
! TTTGGTGAGCTTTGATTGCA+AGG + chr4.2:10537158-10537177 None:intergenic 40.0%
!! AGTCTTACTGGTTTGATCAC+CGG - chr4.2:10536660-10536679 MS.gene70354:CDS 40.0%
!! ATGGGAGTGGTTTTTGAAGA+AGG - chr4.2:10537732-10537751 MS.gene70354:CDS 40.0%
!! CTATAAACAAAGGTGTCTCC+AGG + chr4.2:10535440-10535459 None:intergenic 40.0%
!! GATAGAATCAGGTTGAACTG+CGG - chr4.2:10536476-10536495 MS.gene70354:intron 40.0%
!!! AAAATTTTGGTGCTGCCCTT+AGG + chr4.2:10534979-10534998 None:intergenic 40.0%
!!! GGACATGATTTTTGGGTACT+TGG - chr4.2:10537531-10537550 MS.gene70354:intron 40.0%
!!! TCTATCGGTTTTATAGGGCT+GGG + chr4.2:10536462-10536481 None:intergenic 40.0%
!!! TTCTATCGGTTTTATAGGGC+TGG + chr4.2:10536463-10536482 None:intergenic 40.0%
AAACCAGCCAGTGTAACACA+TGG - chr4.2:10536413-10536432 MS.gene70354:intron 45.0%
AAATCATAGCCATCAGGTGG+AGG + chr4.2:10537354-10537373 None:intergenic 45.0%
AATTTCCACAAAGCTGAGGC+TGG - chr4.2:10535028-10535047 MS.gene70354:CDS 45.0%
ACACATCCATGGCATCTTCA+TGG - chr4.2:10537510-10537529 MS.gene70354:intron 45.0%
ACTACCCAAACCATCCAATG+AGG - chr4.2:10537067-10537086 MS.gene70354:intron 45.0%
CAAAGGGAAGCAGGAGTTTA+TGG - chr4.2:10535799-10535818 MS.gene70354:CDS 45.0%
CAGAAAACCTTGCCATCCAT+TGG - chr4.2:10535206-10535225 MS.gene70354:CDS 45.0%
CCATGAATCAGAAGTGACTG+AGG + chr4.2:10536087-10536106 None:intergenic 45.0%
CCATTGGCATGGTATTAGAC+AGG - chr4.2:10535222-10535241 MS.gene70354:CDS 45.0%
CCTACCATTCAAGCACAAGA+AGG - chr4.2:10535145-10535164 MS.gene70354:CDS 45.0%
CCTCAGTCACTTCTGATTCA+TGG - chr4.2:10536084-10536103 MS.gene70354:intron 45.0%
CCTGTCTAATACCATGCCAA+TGG + chr4.2:10535225-10535244 None:intergenic 45.0%
CCTTCTGTTCCATCAAACCA+AGG + chr4.2:10535395-10535414 None:intergenic 45.0%
CCTTCTTGTGCTTGAATGGT+AGG + chr4.2:10535148-10535167 None:intergenic 45.0%
GAAAATGCCTAAACCAGGTG+GGG + chr4.2:10537043-10537062 None:intergenic 45.0%
GAAGCTACCTTCATCCATCA+TGG - chr4.2:10537770-10537789 MS.gene70354:CDS 45.0%
GAGACGACTGTTTGCGAAAA+GGG + chr4.2:10536186-10536205 None:intergenic 45.0%
GATCAGATAATCCTGGTGTG+TGG - chr4.2:10537670-10537689 MS.gene70354:CDS 45.0%
GATGAGTTACAAACACCAGC+AGG + chr4.2:10536147-10536166 None:intergenic 45.0%
GGCAAAAGCAAAGGCTTCTT+AGG - chr4.2:10537798-10537817 MS.gene70354:CDS 45.0%
GGTGAATTTCCACAAAGCTG+AGG - chr4.2:10535024-10535043 MS.gene70354:CDS 45.0%
TATGGAATGCAAAGGGAAGC+AGG - chr4.2:10535790-10535809 MS.gene70354:CDS 45.0%
TCATGTCCATGAAGATGCCA+TGG + chr4.2:10537519-10537538 None:intergenic 45.0%
TGAGACGACTGTTTGCGAAA+AGG + chr4.2:10536187-10536206 None:intergenic 45.0%
TGGCATGGTATTAGACAGGT+TGG - chr4.2:10535226-10535245 MS.gene70354:CDS 45.0%
! AAGGTGTCTCCAGGTAGTAT+AGG + chr4.2:10535431-10535450 None:intergenic 45.0%
! AATCCCTTTTCAATGGGTGG+GGG - chr4.2:10535939-10535958 MS.gene70354:intron 45.0%
! ACTCTATCTGAGGTTGTTGG+AGG + chr4.2:10537195-10537214 None:intergenic 45.0%
! ATGGGTTGTGGCAAAAGCAA+AGG - chr4.2:10537789-10537808 MS.gene70354:CDS 45.0%
! CAATCCCTTTTCAATGGGTG+GGG - chr4.2:10535938-10535957 MS.gene70354:intron 45.0%
! CAATTTAGGGTTAAGGGTCG+TGG - chr4.2:10536602-10536621 MS.gene70354:CDS 45.0%
! CCTTGGTTTGATGGAACAGA+AGG - chr4.2:10535392-10535411 MS.gene70354:CDS 45.0%
! GCAATCCCTTTTCAATGGGT+GGG - chr4.2:10535937-10535956 MS.gene70354:intron 45.0%
! TGCAATCCCTTTTCAATGGG+TGG - chr4.2:10535936-10535955 MS.gene70354:intron 45.0%
! TTTTCGCAAACAGTCGTCTC+AGG - chr4.2:10536186-10536205 MS.gene70354:intron 45.0%
!! AAAAATATTTATATCTAATT+TGG - chr4.2:10535720-10535739 MS.gene70354:CDS 5.0%
!! AAATAAATAAATGAATAAAT+AGG + chr4.2:10536561-10536580 None:intergenic 5.0%
AACCAAACACCTCCACCTGA+TGG - chr4.2:10537342-10537361 MS.gene70354:CDS 50.0%
AACCTTGCCATCCATTGGCA+TGG - chr4.2:10535211-10535230 MS.gene70354:CDS 50.0%
AGAAACAGAACAACCCCACC+TGG - chr4.2:10537027-10537046 MS.gene70354:intron 50.0%
AGAACAACCCCACCTGGTTT+AGG - chr4.2:10537033-10537052 MS.gene70354:intron 50.0%
CAAACACCAGCAGGTAAGCT+TGG + chr4.2:10536138-10536157 None:intergenic 50.0%
CATGGGACAATGTTGAGTCC+AGG - chr4.2:10537121-10537140 MS.gene70354:intron 50.0%
GAACCATGTGTTACACTGGC+TGG + chr4.2:10536419-10536438 None:intergenic 50.0%
GACTCAACATTGTCCCATGC+TGG + chr4.2:10537120-10537139 None:intergenic 50.0%
GCGACCTCATTGGATGGTTT+GGG + chr4.2:10537074-10537093 None:intergenic 50.0%
GGCTGAACCATGTGTTACAC+TGG + chr4.2:10536423-10536442 None:intergenic 50.0%
GTTCCCCCACCCATTGAAAA+GGG + chr4.2:10535945-10535964 None:intergenic 50.0%
TAACACCAGCCTCAGCTTTG+TGG + chr4.2:10535036-10535055 None:intergenic 50.0%
TACCATGCCAATGGATGGCA+AGG + chr4.2:10535216-10535235 None:intergenic 50.0%
TCAACATTGTCCCATGCTGG+TGG + chr4.2:10537117-10537136 None:intergenic 50.0%
TGTGCTTGAATGGTAGGTCC+TGG + chr4.2:10535142-10535161 None:intergenic 50.0%
TGTTGTGAAATCGGACCGAC+TGG - chr4.2:10536381-10536400 MS.gene70354:intron 50.0%
! CAAGATAAGGGGTGTGAGGT+AGG + chr4.2:10537290-10537309 None:intergenic 50.0%
! CCTTCATCCATCATGGGTTG+TGG - chr4.2:10537777-10537796 MS.gene70354:CDS 50.0%
! GCAACAAGATAAGGGGTGTG+AGG + chr4.2:10537294-10537313 None:intergenic 50.0%
! GCTTTTGCCACAACCCATGA+TGG + chr4.2:10537787-10537806 None:intergenic 50.0%
!! CCACAACCCATGATGGATGA+AGG + chr4.2:10537780-10537799 None:intergenic 50.0%
!! TGCAAGGCTTTGAGTATGCC+TGG + chr4.2:10537142-10537161 None:intergenic 50.0%
!! TGCGAAAAGGGAGAGCATTC+AGG + chr4.2:10536174-10536193 None:intergenic 50.0%
ACATTGTCCCATGCTGGTGG+AGG + chr4.2:10537114-10537133 None:intergenic 55.0%
CAATGGAAAGCCCACACACC+AGG + chr4.2:10537684-10537703 None:intergenic 55.0%
GGCGACCTCATTGGATGGTT+TGG + chr4.2:10537075-10537094 None:intergenic 55.0%
GGTTCCCCCACCCATTGAAA+AGG + chr4.2:10535946-10535965 None:intergenic 55.0%
GTAACACATGGTTCAGCCGC+AGG - chr4.2:10536425-10536444 MS.gene70354:intron 55.0%
!! AGCCATCAGGTGGAGGTGTT+TGG + chr4.2:10537347-10537366 None:intergenic 55.0%
!! GATGTTGGTGGCGACCTCAT+TGG + chr4.2:10537084-10537103 None:intergenic 55.0%
!! TTGGTGGCGACCTCATTGGA+TGG + chr4.2:10537080-10537099 None:intergenic 55.0%
TCCACCACCTCCACCAGCAT+GGG - chr4.2:10537104-10537123 MS.gene70354:intron 60.0%
TTGTCCCATGCTGGTGGAGG+TGG + chr4.2:10537111-10537130 None:intergenic 60.0%
! CTGGGAGTGTGAACTGCCTG+CGG + chr4.2:10536444-10536463 None:intergenic 60.0%
!! GGAGGTGGTGGAGATGTTGG+TGG + chr4.2:10537096-10537115 None:intergenic 60.0%
!! GGTGGAGGTGGTGGAGATGT+TGG + chr4.2:10537099-10537118 None:intergenic 60.0%
CTCCACCACCTCCACCAGCA+TGG - chr4.2:10537103-10537122 MS.gene70354:intron 65.0%
TCCCATGCTGGTGGAGGTGG+TGG + chr4.2:10537108-10537127 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 10534956 10537830 10534956 ID=MS.gene70354
chr4.2 mRNA 10534956 10537830 10534956 ID=MS.gene70354.t1;Parent=MS.gene70354
chr4.2 exon 10537543 10537830 10537543 ID=MS.gene70354.t1.exon1;Parent=MS.gene70354.t1
chr4.2 CDS 10537543 10537830 10537543 ID=cds.MS.gene70354.t1;Parent=MS.gene70354.t1
chr4.2 exon 10537311 10537403 10537311 ID=MS.gene70354.t1.exon2;Parent=MS.gene70354.t1
chr4.2 CDS 10537311 10537403 10537311 ID=cds.MS.gene70354.t1;Parent=MS.gene70354.t1
chr4.2 exon 10536817 10537023 10536817 ID=MS.gene70354.t1.exon3;Parent=MS.gene70354.t1
chr4.2 CDS 10536817 10537023 10536817 ID=cds.MS.gene70354.t1;Parent=MS.gene70354.t1
chr4.2 exon 10536514 10536696 10536514 ID=MS.gene70354.t1.exon4;Parent=MS.gene70354.t1
chr4.2 CDS 10536514 10536696 10536514 ID=cds.MS.gene70354.t1;Parent=MS.gene70354.t1
chr4.2 exon 10534956 10535915 10534956 ID=MS.gene70354.t1.exon5;Parent=MS.gene70354.t1
chr4.2 CDS 10534956 10535915 10534956 ID=cds.MS.gene70354.t1;Parent=MS.gene70354.t1
Gene Sequence

>MS.gene70354

ATGGTTCAACTTCAACTAAGCCTAAGGGCAGCACCAAAATTTTTGTTTCTATGTTTCTTTCTAATTCTGGTGAATTTCCACAAAGCTGAGGCTGGTGTTAGACATTATAAATGGGAGGTGAAGTATGATTATAGGTCCCCTGATTGCTATAAGAAACTTGTTATAACCATTAATGGAAAAACTCCAGGACCTACCATTCAAGCACAAGAAGGTGATACTGTTGTAGTTGAAGTTAACAACAAATTGCTCACAGAAAACCTTGCCATCCATTGGCATGGTATTAGACAGGTTGGCCTTTACTAGTCCAAAATATTTTAAATTTAACAGGAATAAATATAAAATTAGATAAAAAAAAATCTATTCATTTGGCCTATATTTGGTGCTTAATGATTAATATATAATGACTGATGTGGTTTTTGTATGACAGATTGGAACTCCTTGGTTTGATGGAACAGAAGGGGTATCTCAATGTCCTATACTACCTGGAGACACCTTTGTTTATAGATTTGTTGTTGATAGGGTAAGTTGTCAAAATGAAAAGATGAATGAATTCGTGCATGTTTAGAATCAATATGAGTTTGAAGAAATTATGGTGGCACCATGATTTTATTGAGGCTTTAAAGTGTAGCTTTCGTCAAAATCTTAGTGACATGCCATGATTTTGCCAAACTCACAGTTGATCATCAAACATGATCTCTTCATTGATCCGTTGTTCTTCAATTTCAACACTAACAAACTATATTTATATAATCAATCATAGCATAAAAAATATTTATATCTAATTTGGTTACATATTTAATGTTACAGCCTGGTACATATCTTTATCATGCTCACTATGGAATGCAAAGGGAAGCAGGAGTTTATGGAATGATTCGTGTGGCACCTAATGAACCTGAACCTTTTTCTTATGACTTTGATAGAAGCATTATCTTGAATGATTGGTACCATAAGAGTACTTATGAACAATCTGCTGGATTGTCTGCAATCCCTTTTCAATGGGTGGGGGAACCAGATGTAAGAATTAGATGGATATACTAATCAGAAAACAAATTCAAAATTTTATATCTAAATTTTCTTTCTTCAATTGTCTAATTGTTGGTTTTATTGTTTCTTTGTATATTTGGTTTCCCTCAGTCACTTCTGATTCATGGAAAAGGAAGATACGACTGTTCGTTATTACCAAGCTTACCTGCTGGTGTTTGTAACTCATCAAATCCTGAATGCTCTCCCTTTTCGCAAACAGTCGTCTCAGGAAAAACATACAGAATTAGAGTTGCTAGCTTGACTGCTTTATCAGCACTCAGTTTCCAAATAGAGGTAATCATTCATTCACTCAATTATTCTAAGTATCATCCAAGTTTTTAAACAAGGTCTGCGACAATAATCTCAGTTGCGTTACCATCGTTTTTGATGTACACACGACAATGTTGTGAAATCGGACCGACTGGTTTAACTGAAAACCAGCCAGTGTAACACATGGTTCAGCCGCAGGCAGTTCACACTCCCAGCCCTATAAAACCGATAGAATCAGGTTGAACTGCGGTTCAACAAGAATTGGTTCAACCAGTCTGTCTTCTTTATACAAAATATAAATTTTTAATGCCTATTTATTCATTTATTTATTTCTTAGTCATATGGTTGAACTACAATTTAGGGTTAAGGGTCGTGGCTAAACCGATTAAACCAATTGAATCATGACCTAAAAGTCTTACTGGTTTGATCACCGGTCTTGTTTTGACAACAATGATCTACTATCGCAAAATGACTACGATTAAGGTCACATCAGTCATATATGATTCTTTGCAATATCAAAAGATTCTGACCAGACTATTACCACAACTGCATTTTAGCCTTTGTTTAATTTTTCTCGCTTTTTACATGCAGAACTAATTATGACATGTTTGTAATGATGCAGGGCCACAACATGACTGTTGTTGAAGCAGATGGACACTATGTTGATCCTTTTGTGGTTAAGAATCTCTACATATACTCAGGTGAAACATACTCAGTTCTAGTCAAAACTGATCAAGACCCATCAAGAAACTACTGGATCACCTCAAATGTTGTTAGCAGAAACAGAACAACCCCACCTGGTTTAGGCATTTTCAACTACTACCCAAACCATCCAATGAGGTCGCCACCAACATCTCCACCACCTCCACCAGCATGGGACAATGTTGAGTCCAGGCATACTCAAAGCCTTGCAATCAAAGCTCACCAAAATTACACAATCAAACCTCCAACAACCTCAGATAGAGTCATAGTTTTGCTCAACACACAAAACACTATAGACAATGTTCGTCATTGGTCTGTTAACAACGTATCATTTTTCCTACCTCACACCCCTTATCTTGTTGCACTTAAAGAGAATATAACTGGTGAATTCAACCAAACACCTCCACCTGATGGCTATGATTTTCATAATTACGATATTTTCAGTGTGGCTAACAACACAAATGCTACTTCTAGCAATGGAATTTATAGGCTGAAGTTCAATACAACTGTGGATATTATACTACAAAATGCAAACACTATGAATAAAAATAATAGTGAGACACATCCATGGCATCTTCATGGACATGATTTTTGGGTACTTGGATATGGAAAGGGTAAGTTTGACGTGAACAAAGACCCAAAAAATTACAATTTGGTGAACCCCATTATGAAGAATACTGTGCCAGTACACTTATTTGGTTGGACTGCTTTGAGGTTTAGATCAGATAATCCTGGTGTGTGGGCTTTCCATTGTCATATAGAGTCTCATTTCTATATGGGAATGGGAGTGGTTTTTGAAGAAGGAATTGAAAGGGTTGGGAAGCTACCTTCATCCATCATGGGTTGTGGCAAAAGCAAAGGCTTCTTAGGCCATAATTAA
Protein sequence

>MS.gene70354.t1

MVQLQLSLRAAPKFLFLCFFLILVNFHKAEAGVRHYKWEVKYDYRSPDCYKKLVITINGKTPGPTIQAQEGDTVVVEVNNKLLTENLAIHWHGIRQIGTPWFDGTEGVSQCPILPGDTFVYRFVVDRPGTYLYHAHYGMQREAGVYGMIRVAPNEPEPFSYDFDRSIILNDWYHKSTYEQSAGLSAIPFQWVGEPDSLLIHGKGRYDCSLLPSLPAGVCNSSNPECSPFSQTVVSGKTYRIRVASLTALSALSFQIEGHNMTVVEADGHYVDPFVVKNLYIYSGETYSVLVKTDQDPSRNYWITSNVVSRNRTTPPGLGIFNYYPNHPMRSPPTSPPPPPAWDNVESRHTQSLAIKAHQNYTIKPPTTSDRVIVLLNTQNTIDNVRHWSVNNVSFFLPHTPYLVALKENITGEFNQTPPPDGYDFHNYDIFSVANNTNATSSNGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHGHDFWVLGYGKGKFDVNKDPKNYNLVNPIMKNTVPVHLFGWTALRFRSDNPGVWAFHCHIESHFYMGMGVVFEEGIERVGKLPSSIMGCGKSKGFLGHN