AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28968

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28968.t1 MTR_8g091390 93.103 435 16 3 107 527 97 531 0.0 801
MS.gene28968.t1 MTR_3g078740 44.516 465 207 13 108 527 90 548 5.29e-123 373
MS.gene28968.t1 MTR_3g078700 44.521 438 199 7 107 527 89 499 1.42e-120 365
MS.gene28968.t1 MTR_3g078730 45.000 440 194 8 107 527 88 498 1.96e-120 365
MS.gene28968.t1 MTR_5g020600 33.036 448 247 17 111 527 100 525 1.44e-48 177
MS.gene28968.t1 MTR_5g020620 31.614 446 255 14 111 527 96 520 1.60e-46 171
MS.gene28968.t1 MTR_5g081810 32.000 450 251 17 109 527 63 488 4.42e-45 166
MS.gene28968.t1 MTR_5g069680 31.320 447 259 16 109 527 91 517 3.49e-42 159
MS.gene28968.t1 MTR_3g462760 30.134 448 262 16 109 527 90 515 4.83e-41 155
MS.gene28968.t1 MTR_5g083360 29.526 464 261 17 107 527 86 526 8.29e-41 155
MS.gene28968.t1 MTR_3g071890 30.369 461 245 18 109 524 97 526 2.19e-40 154
MS.gene28968.t1 MTR_7g062250 30.369 461 258 19 109 527 98 537 6.41e-39 150
MS.gene28968.t1 MTR_8g027375 29.360 453 262 15 111 527 100 530 9.47e-39 149
MS.gene28968.t1 MTR_7g062310 28.632 468 263 19 107 527 109 552 3.20e-38 148
MS.gene28968.t1 MTR_7g458880 27.957 465 263 17 111 527 99 539 8.39e-38 147
MS.gene28968.t1 MTR_5g073210 28.322 459 267 14 106 527 86 519 3.78e-36 142
MS.gene28968.t1 MTR_2g008330 29.067 461 259 17 110 527 103 538 1.06e-35 140
MS.gene28968.t1 MTR_7g060460 28.510 463 264 17 111 527 99 540 3.15e-35 139
MS.gene28968.t1 MTR_7g058690 27.706 462 268 17 111 527 99 539 4.62e-35 139
MS.gene28968.t1 MTR_3g101640 29.590 463 254 21 110 527 95 530 7.56e-35 138
MS.gene28968.t1 MTR_7g065980 27.729 458 272 15 111 527 91 530 2.55e-34 137
MS.gene28968.t1 MTR_7g065970 27.586 464 268 18 109 527 89 529 3.56e-33 133
MS.gene28968.t1 MTR_3g101635 29.322 457 261 19 110 527 92 525 8.01e-33 132
MS.gene28968.t1 MTR_4g064530 28.728 456 265 17 111 527 110 544 4.56e-32 130
MS.gene28968.t1 MTR_4g019225 28.112 466 264 19 109 527 90 531 1.10e-31 129
MS.gene28968.t1 MTR_4g064530 28.478 460 259 19 111 526 110 543 1.13e-31 129
MS.gene28968.t1 MTR_5g018270 26.244 442 252 13 111 527 97 489 2.23e-30 125
MS.gene28968.t1 MTR_4g132320 26.503 449 246 13 111 527 97 493 4.78e-30 124
MS.gene28968.t1 MTR_4g049390 26.726 449 245 14 111 527 93 489 5.16e-30 124
MS.gene28968.t1 MTR_8g479390 26.966 445 248 17 111 527 97 492 4.56e-29 121
MS.gene28968.t1 MTR_4g048350 26.185 443 254 13 111 527 93 488 9.90e-29 120
MS.gene28968.t1 MTR_2g088080 25.612 449 250 12 111 527 15 411 2.98e-28 117
MS.gene28968.t1 MTR_3g083440 27.130 446 254 17 111 527 91 494 5.85e-28 117
MS.gene28968.t1 MTR_2g088080 25.612 449 250 12 111 527 96 492 7.37e-28 117
MS.gene28968.t1 MTR_4g075690 25.000 440 256 13 113 527 101 491 9.91e-28 117
MS.gene28968.t1 MTR_3g083390 27.130 446 254 17 111 527 91 494 1.03e-27 117
MS.gene28968.t1 MTR_3g083450 27.130 446 254 17 111 527 92 495 1.32e-27 117
MS.gene28968.t1 MTR_3g083400 27.130 446 254 17 111 527 91 494 1.56e-27 116
MS.gene28968.t1 MTR_3g008580 26.398 447 266 16 111 526 95 509 2.07e-27 116
MS.gene28968.t1 MTR_1g090707 25.618 445 264 16 111 527 90 495 2.34e-27 116
MS.gene28968.t1 MTR_3g083410 26.682 446 256 17 111 527 55 458 2.74e-27 115
MS.gene28968.t1 MTR_3g115050 24.722 449 247 16 116 527 98 492 3.20e-27 115
MS.gene28968.t1 MTR_3g083430 26.906 446 255 17 111 527 91 494 1.19e-26 114
MS.gene28968.t1 MTR_1g026410 26.244 442 237 15 121 527 104 491 2.02e-26 113
MS.gene28968.t1 MTR_4g083210 26.531 441 259 15 111 527 91 490 6.81e-26 111
MS.gene28968.t1 MTR_5g081790 41.667 144 75 3 386 526 283 420 2.05e-25 108
MS.gene28968.t1 MTR_7g051440 27.556 450 257 19 111 527 95 508 4.68e-25 109
MS.gene28968.t1 MTR_4g015120 40.845 142 80 2 386 527 379 516 1.22e-24 107
MS.gene28968.t1 MTR_4g101650 27.051 451 264 17 109 527 91 508 2.30e-24 107
MS.gene28968.t1 MTR_4g083190 24.658 438 275 13 111 527 93 496 1.57e-23 104
MS.gene28968.t1 MTR_4g075690 24.810 395 224 12 113 482 101 447 2.43e-23 103
MS.gene28968.t1 MTR_8g479380 23.884 448 262 16 109 527 93 490 1.73e-21 98.2
MS.gene28968.t1 MTR_4g075210 53.846 78 31 2 5 78 79 155 2.80e-21 91.3
MS.gene28968.t1 MTR_3g079660 52.000 50 24 0 478 527 5 54 3.43e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28968.t1 AT4G39830 63.288 444 141 6 106 527 1 444 0.0 582
MS.gene28968.t1 AT4G39830 63.431 443 140 6 107 527 99 541 0.0 582
MS.gene28968.t1 AT4G39830 63.431 443 140 6 107 527 4 446 0.0 580
MS.gene28968.t1 AT4G39830 61.174 443 137 7 107 527 4 433 0.0 545
MS.gene28968.t1 AT5G21100 43.167 461 236 9 89 527 66 522 1.25e-123 374
MS.gene28968.t1 AT5G21105 44.227 459 227 10 93 526 84 538 1.26e-123 373
MS.gene28968.t1 AT5G21105 44.921 443 218 9 107 527 101 539 2.46e-123 374
MS.gene28968.t1 AT5G21105 45.161 341 168 7 202 527 12 348 6.74e-89 279
MS.gene28968.t1 AT5G58910 31.291 457 252 17 109 527 50 482 1.61e-44 165
MS.gene28968.t1 AT5G58910 31.291 457 252 17 109 527 94 526 2.36e-44 165
MS.gene28968.t1 AT5G58910 31.291 457 252 17 109 527 93 525 2.62e-44 165
MS.gene28968.t1 AT1G18140 29.806 463 266 17 109 527 93 540 1.44e-43 163
MS.gene28968.t1 AT5G05390 30.152 461 257 19 107 527 89 524 2.41e-43 162
MS.gene28968.t1 AT2G38080 31.081 444 268 14 107 527 89 517 4.10e-43 161
MS.gene28968.t1 AT5G48100 29.870 462 254 21 110 527 87 522 1.63e-38 149
MS.gene28968.t1 AT5G01050 28.228 457 265 17 111 527 94 527 5.76e-38 147
MS.gene28968.t1 AT5G01190 29.759 457 255 15 109 527 75 503 1.36e-37 145
MS.gene28968.t1 AT5G01190 29.759 457 255 15 109 527 89 517 1.91e-37 145
MS.gene28968.t1 AT5G60020 29.424 469 256 20 111 527 91 536 4.24e-37 145
MS.gene28968.t1 AT5G60020 29.424 469 256 20 111 527 59 504 5.25e-37 144
MS.gene28968.t1 AT5G01040 29.451 455 260 19 111 527 94 525 6.31e-37 144
MS.gene28968.t1 AT5G60020 29.274 468 256 20 111 526 91 535 7.92e-37 144
MS.gene28968.t1 AT2G46570 28.384 458 260 17 111 527 13 443 1.38e-36 142
MS.gene28968.t1 AT2G29130 28.446 457 267 17 111 527 96 532 1.91e-36 143
MS.gene28968.t1 AT2G46570 28.384 458 260 17 111 527 98 528 3.97e-36 142
MS.gene28968.t1 AT5G09360 29.462 465 250 20 107 527 98 528 3.13e-35 139
MS.gene28968.t1 AT2G30210 27.885 520 300 21 60 527 33 529 2.31e-34 137
MS.gene28968.t1 AT5G07130 28.112 466 261 19 110 527 4 443 9.13e-34 134
MS.gene28968.t1 AT5G07130 28.112 466 261 19 110 527 4 443 9.13e-34 134
MS.gene28968.t1 AT5G07130 28.112 466 261 19 110 527 89 528 1.76e-33 134
MS.gene28968.t1 AT3G09220 28.105 459 266 18 109 526 90 525 4.92e-33 133
MS.gene28968.t1 AT1G75790 28.571 448 243 19 111 527 93 494 8.46e-33 132
MS.gene28968.t1 AT2G40370 27.848 474 260 18 109 527 93 539 1.39e-32 132
MS.gene28968.t1 AT3G09220 28.000 450 260 18 118 526 2 428 2.97e-32 129
MS.gene28968.t1 AT5G66920 25.909 440 258 13 111 527 103 497 5.29e-32 129
MS.gene28968.t1 AT4G22010 27.252 444 250 15 111 527 92 489 3.70e-30 124
MS.gene28968.t1 AT2G23630 26.164 451 244 18 111 527 101 496 1.31e-29 122
MS.gene28968.t1 AT2G23630 26.164 451 244 18 111 527 96 491 1.53e-29 122
MS.gene28968.t1 AT1G21850 26.697 442 245 17 113 527 100 489 1.82e-29 122
MS.gene28968.t1 AT1G76160 26.292 445 244 15 113 527 99 489 9.28e-29 120
MS.gene28968.t1 AT1G55570 26.697 442 262 13 111 527 95 499 1.35e-28 120
MS.gene28968.t1 AT4G28090 27.232 448 240 19 113 527 99 493 7.84e-28 117
MS.gene28968.t1 AT4G12420 27.455 448 262 18 111 527 91 506 1.06e-27 117
MS.gene28968.t1 AT4G12420 27.455 448 262 18 111 527 91 506 1.06e-27 117
MS.gene28968.t1 AT1G21860 25.113 442 252 15 113 527 100 489 8.00e-27 114
MS.gene28968.t1 AT4G25240 26.786 448 264 16 111 527 95 509 9.06e-27 114
MS.gene28968.t1 AT4G25240 26.786 448 264 16 111 527 95 509 9.06e-27 114
MS.gene28968.t1 AT4G37160 24.664 446 262 15 109 527 95 493 4.53e-26 112
MS.gene28968.t1 AT3G13390 25.670 448 259 15 111 527 94 498 1.87e-25 110
MS.gene28968.t1 AT5G48450 26.233 446 260 15 111 527 95 500 3.01e-24 107
MS.gene28968.t1 AT5G48450 25.734 443 266 14 111 527 95 500 3.02e-24 107
MS.gene28968.t1 AT3G13400 25.285 439 271 14 111 527 93 496 4.22e-24 106
MS.gene28968.t1 AT3G13400 25.285 439 271 14 111 527 93 496 4.22e-24 106
MS.gene28968.t1 AT5G51480 25.778 450 267 17 111 527 94 509 4.74e-24 106
MS.gene28968.t1 AT1G41830 23.649 444 259 15 112 527 99 490 9.77e-24 105
MS.gene28968.t1 AT4G38420 24.494 445 259 16 113 527 101 498 1.47e-19 92.4
MS.gene28968.t1 AT5G03260 34.615 208 106 9 111 302 92 285 6.44e-18 87.4
MS.gene28968.t1 AT4G37160 23.691 401 233 14 109 482 95 449 7.61e-18 86.7
MS.gene28968.t1 AT4G38420 24.009 429 249 16 113 511 101 482 1.50e-16 82.8
MS.gene28968.t1 AT4G38420 24.250 400 227 15 113 482 101 454 4.21e-15 78.2

Find 107 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCACTTGTTGATCATTA+TGG 0.178187 4.4:+34850551 None:intergenic
AATGAATGATTACCTCTATT+TGG 0.179597 4.4:+34848184 None:intergenic
CCTCAGTCACTTCTGATTCA+TGG 0.203458 4.4:-34848363 MS.gene28968:intron
GGCACAATATTCTTCATAAT+GGG 0.258488 4.4:+34846851 None:intergenic
GGTTTGTATGCATGCTATTT+TGG 0.262041 4.4:-34850013 MS.gene28968:intron
TTAATCTTCTAAATACTAAC+TGG 0.285797 4.4:-34850035 MS.gene28968:CDS
GGCGACCTCATTGGATGGTT+TGG 0.294439 4.4:+34847391 None:intergenic
TTAGTATTTAGAAGATTAAT+AGG 0.297642 4.4:+34850039 None:intergenic
TGGCACAATATTCTTCATAA+TGG 0.303902 4.4:+34846850 None:intergenic
CAAAGGGAAGCAGGGGTATA+TGG 0.305479 4.4:-34848650 MS.gene28968:CDS
GAGGTTTGATTGTGTAATTT+TGG 0.312038 4.4:+34847290 None:intergenic
CATTTGGTTGGACTGCTTTG+AGG 0.330733 4.4:-34846820 MS.gene28968:CDS
CTATCAAAGTCATAAGAAAA+AGG 0.331498 4.4:+34848596 None:intergenic
CTGCTTCCCTTTGCATTCCA+TGG 0.346356 4.4:+34848660 None:intergenic
TGTGAAAAGGGAGAGCATTC+AGG 0.354951 4.4:+34848276 None:intergenic
GCGACCTCATTGGATGGTTT+GGG 0.364061 4.4:+34847392 None:intergenic
TTTATCGATTTGTTGTTGAT+AGG 0.375099 4.4:-34848998 MS.gene28968:CDS
AGCCATCAGGTGGAGGTGTT+TGG 0.375855 4.4:+34847119 None:intergenic
TGGGTACTTGGATATGGAAA+TGG 0.389514 4.4:-34846920 MS.gene28968:CDS
GTGATCCAGTAGTTTCTTGA+TGG 0.389887 4.4:+34847475 None:intergenic
TCCGCCACCTCCACCAGCTT+GGG 0.398311 4.4:-34847359 MS.gene28968:CDS
GCACTTAAAGAGAATATAAC+TGG 0.408038 4.4:-34847151 MS.gene28968:CDS
ATTGTTCATAAGTACTCTTA+TGG 0.412906 4.4:+34848549 None:intergenic
ACGCATCCATGGCATCTTCA+TGG 0.422581 4.4:-34846953 MS.gene28968:CDS
GTTTCTGCTGACAACATTTG+AGG 0.423513 4.4:+34847453 None:intergenic
AGATAACTTTGTCTGATGAT+TGG 0.429809 4.4:-34850531 MS.gene28968:CDS
GATAACTTTGTCTGATGATT+GGG 0.432274 4.4:-34850530 MS.gene28968:CDS
CTCCGCCACCTCCACCAGCT+TGG 0.437173 4.4:-34847360 MS.gene28968:CDS
TACACAAGGACACGATGTTC+CGG 0.439183 4.4:-34850367 MS.gene28968:CDS
GAGACGACTGTTTGTGAAAA+GGG 0.447475 4.4:+34848264 None:intergenic
TTTAAGTGCAACAAGATAAG+AGG 0.449917 4.4:+34847165 None:intergenic
ACTCTATCTGAGGTTGTTGG+AGG 0.454902 4.4:+34847271 None:intergenic
GGTGGAGGTGGCGGAGATGT+TGG 0.460069 4.4:+34847367 None:intergenic
TGAGACGACTGTTTGTGAAA+AGG 0.460440 4.4:+34848263 None:intergenic
CTATAGACGGTGTTCGTCAT+TGG 0.461142 4.4:-34847222 MS.gene28968:CDS
CACGACAGATTGGAACTCCT+TGG 0.462498 4.4:-34849076 MS.gene28968:intron
TTATCGATTTGTTGTTGATA+GGG 0.462550 4.4:-34848997 MS.gene28968:intron
AGAACAACCCCGCCTGGTTT+AGG 0.465849 4.4:-34847430 MS.gene28968:CDS
TCCCAAGCTGGTGGAGGTGG+CGG 0.469652 4.4:+34847358 None:intergenic
AGCACTCAGTTTCCAAATAG+AGG 0.474284 4.4:-34848196 MS.gene28968:intron
TTGTCCCAAGCTGGTGGAGG+TGG 0.474906 4.4:+34847355 None:intergenic
AGTACTTATGAACAATCTAC+TGG 0.476586 4.4:-34848542 MS.gene28968:CDS
TCACTTCTGATTCATGGAAA+AGG 0.477199 4.4:-34848357 MS.gene28968:CDS
CATGGAATGCAAAGGGAAGC+AGG 0.480781 4.4:-34848659 MS.gene28968:CDS
AGGTTTCGATCGGATAATCC+TGG 0.493226 4.4:-34846800 MS.gene28968:CDS
GACTGCTTTGAGGTTTCGAT+CGG 0.497349 4.4:-34846810 MS.gene28968:CDS
TGCCAGTGCACTCATTTGGT+TGG 0.498134 4.4:-34846832 MS.gene28968:CDS
TAGAGATTCTTACCCAGAAA+AGG 0.499524 4.4:+34847547 None:intergenic
ATGACTCTATCTGAGGTTGT+TGG 0.501248 4.4:+34847268 None:intergenic
TTTGGTGAGCTTTGATTGCA+AGG 0.510484 4.4:+34847308 None:intergenic
ACATCGTGTCCTTGTGTATG+TGG 0.512622 4.4:+34850372 None:intergenic
GACTCAACATTGTCCCAAGC+TGG 0.516390 4.4:+34847346 None:intergenic
CGCTGGATTTAACGATAACA+TGG 0.516975 4.4:-34850443 MS.gene28968:CDS
TGCTCACCATGGAATGCAAA+GGG 0.517320 4.4:-34848666 MS.gene28968:CDS
ATTGTGCCAGTGCACTCATT+TGG 0.518075 4.4:-34846836 MS.gene28968:CDS
TGATCCAGTAGTTTCTTGAT+GGG 0.518958 4.4:+34847476 None:intergenic
GATGTTGGTGGCGACCTCAT+TGG 0.527521 4.4:+34847382 None:intergenic
CGATAAACAAAGGTGTCTCC+AGG 0.528939 4.4:+34849014 None:intergenic
GGAGGTGGCGGAGATGTTGG+TGG 0.537789 4.4:+34847370 None:intergenic
AAGACCCATCAAGAAACTAC+TGG 0.542638 4.4:-34847480 MS.gene28968:CDS
TTATTAAAATCATAGCCATC+AGG 0.546504 4.4:+34847106 None:intergenic
TGAAAATGCCTAAACCAGGC+GGG 0.549321 4.4:+34847422 None:intergenic
AGAAACAGAACAACCCCGCC+TGG 0.549767 4.4:-34847436 MS.gene28968:CDS
CAAACACCAGCAGGTACGCT+TGG 0.551126 4.4:+34848312 None:intergenic
ACAAATGCTACTTCTAGCAA+TGG 0.556255 4.4:-34847055 MS.gene28968:CDS
CAATGGAAAGCCTACACACC+AGG 0.557128 4.4:+34846782 None:intergenic
GATGAGTTACAAACACCAGC+AGG 0.565048 4.4:+34848303 None:intergenic
ATAATAGTGAGACGCATCCA+TGG 0.567304 4.4:-34846964 MS.gene28968:CDS
TTGGTGGCGACCTCATTGGA+TGG 0.568529 4.4:+34847386 None:intergenic
TAATCTTCTAAATACTAACT+GGG 0.569303 4.4:-34850034 MS.gene28968:CDS
TTATCACCAAGCGTACCTGC+TGG 0.576751 4.4:-34848318 MS.gene28968:CDS
TCATGTCCATGAAGATGCCA+TGG 0.579018 4.4:+34846947 None:intergenic
CAAGATAAGAGGTGTGAGGT+AGG 0.580118 4.4:+34847176 None:intergenic
ACATTGTCCCAAGCTGGTGG+AGG 0.581616 4.4:+34847352 None:intergenic
ATGGAATGCAAAGGGAAGCA+GGG 0.581775 4.4:-34848658 MS.gene28968:CDS
AAATCATAGCCATCAGGTGG+AGG 0.584412 4.4:+34847112 None:intergenic
TCTCAATGTCCTATACTACC+TGG 0.584728 4.4:-34849032 MS.gene28968:CDS
AAGGTGTCTCCAGGTAGTAT+AGG 0.588687 4.4:+34849023 None:intergenic
ATGACTGTTGTTGAAGCAGA+TGG 0.592854 4.4:-34847583 MS.gene28968:CDS
ATTATTGTAAGCCCTGCAGA+TGG 0.594166 4.4:-34850075 MS.gene28968:CDS
GTCCAACCAAATGAGTGCAC+TGG 0.595748 4.4:+34846830 None:intergenic
ATGCTCACCATGGAATGCAA+AGG 0.599562 4.4:-34848667 MS.gene28968:CDS
TCAACATTGTCCCAAGCTGG+TGG 0.600784 4.4:+34847349 None:intergenic
AACCAAACACCTCCACCTGA+TGG 0.600941 4.4:-34847121 MS.gene28968:CDS
AACACACAAAACACTATAGA+CGG 0.600946 4.4:-34847235 MS.gene28968:CDS
GCACAATATTCTTCATAATG+GGG 0.601103 4.4:+34846852 None:intergenic
GAAAATGCCTAAACCAGGCG+GGG 0.606749 4.4:+34847423 None:intergenic
CAAAACTATGACTCTATCTG+AGG 0.612834 4.4:+34847261 None:intergenic
ACTCCATAATGATCAACAAG+TGG 0.612927 4.4:-34850554 MS.gene28968:CDS
ATCGGATAATCCTGGTGTGT+AGG 0.614384 4.4:-34846792 None:intergenic
CATCGTGTCCTTGTGTATGT+GGG 0.625644 4.4:+34850373 None:intergenic
AACAACAAATCGATAAACAA+AGG 0.630677 4.4:+34849004 None:intergenic
TATCTTTATCATGCTCACCA+TGG 0.631127 4.4:-34848677 MS.gene28968:CDS
TGGAATGCAAAGGGAAGCAG+GGG 0.640578 4.4:-34848657 MS.gene28968:CDS
TTAAAATCATAGCCATCAGG+TGG 0.640896 4.4:+34847109 None:intergenic
GCATGATAAAGATATGTACC+AGG 0.647073 4.4:+34848686 None:intergenic
TTGAAAATGCCTAAACCAGG+CGG 0.654564 4.4:+34847421 None:intergenic
CATGTTATCGTTAAATCCAG+CGG 0.659453 4.4:+34850444 None:intergenic
TAGTTGAAAATGCCTAAACC+AGG 0.667626 4.4:+34847418 None:intergenic
GCAACAAGATAAGAGGTGTG+AGG 0.669037 4.4:+34847172 None:intergenic
GATAAGTTACAGAATACCCA+AGG 0.673514 4.4:+34850395 None:intergenic
TAACTTATCCCACATACACA+AGG 0.683285 4.4:-34850381 MS.gene28968:CDS
CCTTCTGTTCCATAAAACCA+AGG 0.696461 4.4:+34849059 None:intergenic
ACTACCCAAACCATCCAATG+AGG 0.700116 4.4:-34847396 MS.gene28968:CDS
GGTATATGGAATGATTCGTG+TGG 0.705433 4.4:-34848636 MS.gene28968:CDS
ACTGAAGTTCAATACAACTG+TGG 0.713693 4.4:-34847023 MS.gene28968:CDS
AAGTTACAGAATACCCAAGG+AGG 0.731298 4.4:+34850398 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTGTTAATATCTATTT+TGG - chr4.4:34848623-34848642 MS.gene28968:CDS 10.0%
!!! ATTATTATCATTATTTTATC+AGG - chr4.4:34847219-34847238 MS.gene28968:CDS 10.0%
!! AATGCTTATAAAAAAACATT+AGG + chr4.4:34847548-34847567 None:intergenic 15.0%
!! TTAGTATTTAGAAGATTAAT+AGG + chr4.4:34847316-34847335 None:intergenic 15.0%
!!! ATTTACTTCTAAAATCTATT+AGG + chr4.4:34848052-34848071 None:intergenic 15.0%
!!! TTATTGTTTCTTTGTATATT+TGG - chr4.4:34848962-34848981 MS.gene28968:intron 15.0%
!!! TTTACTTCTAAAATCTATTA+GGG + chr4.4:34848051-34848070 None:intergenic 15.0%
!!! TTTTCAATTAAAATGTGAAA+TGG - chr4.4:34847149-34847168 MS.gene28968:CDS 15.0%
!! AATGTGAGAAAAATTAAACA+AGG + chr4.4:34849699-34849718 None:intergenic 20.0%
!! AATTAAACAAGGTTAAATTG+TGG + chr4.4:34849688-34849707 None:intergenic 20.0%
!! ATTCCATATCAAAAATTTCA+CGG - chr4.4:34847854-34847873 MS.gene28968:intron 20.0%
!! TAATCTTCTAAATACTAACT+GGG - chr4.4:34847318-34847337 MS.gene28968:CDS 20.0%
!! TAATGTAGTAATAACTACAA+AGG + chr4.4:34847793-34847812 None:intergenic 20.0%
!! TTAAAGCTTCAATAAAATCA+TGG + chr4.4:34848458-34848477 None:intergenic 20.0%
!! TTAATATATAATGACTGATG+TGG - chr4.4:34848247-34848266 MS.gene28968:CDS 20.0%
!! TTAATCTTCTAAATACTAAC+TGG - chr4.4:34847317-34847336 MS.gene28968:CDS 20.0%
!!! ATCATTCAAGTTTTTAAACA+AGG - chr4.4:34849209-34849228 MS.gene28968:intron 20.0%
!!! ATTTGTTTTAAACTACAAAG+TGG - chr4.4:34848198-34848217 MS.gene28968:CDS 20.0%
!!! CATCTTTTGTTATATGAAAT+GGG + chr4.4:34847749-34847768 None:intergenic 20.0%
!!! TAATTATGATATTTTCAGTG+TGG - chr4.4:34850266-34850285 MS.gene28968:intron 20.0%
!!! TCACCAAGTTATAATTTTTT+GGG + chr4.4:34850479-34850498 None:intergenic 20.0%
!!! TCATCTTTTGTTATATGAAA+TGG + chr4.4:34847750-34847769 None:intergenic 20.0%
!!! TTCACCAAGTTATAATTTTT+TGG + chr4.4:34850480-34850499 None:intergenic 20.0%
!!! TTTCGTTTCATTAATTTTAC+TGG - chr4.4:34847188-34847207 MS.gene28968:CDS 20.0%
!!! TTTGTTTTAAACTACAAAGT+GGG - chr4.4:34848199-34848218 MS.gene28968:CDS 20.0%
! AACAACAAATCGATAAACAA+AGG + chr4.4:34848351-34848370 None:intergenic 25.0%
! AAGAATCTCTACATATAATC+AGG - chr4.4:34849817-34849836 MS.gene28968:intron 25.0%
! AATAGATCATGTTTGATCAA+CGG + chr4.4:34848532-34848551 None:intergenic 25.0%
! AATGAATGATTACCTCTATT+TGG + chr4.4:34849171-34849190 None:intergenic 25.0%
! AATTCAACATGAGAGAAAAA+TGG - chr4.4:34846855-34846874 MS.gene28968:CDS 25.0%
! ACTATTATTTACAATCACAG+AGG + chr4.4:34847624-34847643 None:intergenic 25.0%
! AGACCCAAAAAATTATAACT+TGG - chr4.4:34850473-34850492 MS.gene28968:CDS 25.0%
! ATATTGTTGAACCACAATTT+AGG - chr4.4:34849478-34849497 MS.gene28968:intron 25.0%
! ATTGTTCATAAGTACTCTTA+TGG + chr4.4:34848806-34848825 None:intergenic 25.0%
! CAAGTAGAAGAAAATGAAAA+TGG + chr4.4:34847093-34847112 None:intergenic 25.0%
! CGTTAATTGATTAACTAAAC+TGG - chr4.4:34847672-34847691 MS.gene28968:intron 25.0%
! CTATCAAAGTCATAAGAAAA+AGG + chr4.4:34848759-34848778 None:intergenic 25.0%
! TATGCTTAAGAAAGACTTTA+CGG - chr4.4:34847971-34847990 MS.gene28968:intron 25.0%
! TATTGTTGAACCACAATTTA+GGG - chr4.4:34849479-34849498 MS.gene28968:intron 25.0%
! TGTATCATGTTATGAAAAAG+TGG + chr4.4:34847703-34847722 None:intergenic 25.0%
! TTATTAAAATCATAGCCATC+AGG + chr4.4:34850249-34850268 None:intergenic 25.0%
! TTCCATATCAAAAATTTCAC+GGG - chr4.4:34847855-34847874 MS.gene28968:intron 25.0%
! TTGACAAATGCATAAAGAAA+AGG - chr4.4:34847999-34848018 MS.gene28968:intron 25.0%
!! TGATATGGAATATGACTTTT+AGG + chr4.4:34847845-34847864 None:intergenic 25.0%
!! TTCTTCAATTTTCTAATTGC+TGG - chr4.4:34848937-34848956 MS.gene28968:intron 25.0%
!! TTTTCTTTATGCATTTGTCA+AGG + chr4.4:34848000-34848019 None:intergenic 25.0%
!!! AGATTTTAGAAGTAAATGCA+AGG - chr4.4:34848055-34848074 MS.gene28968:intron 25.0%
!!! GAAGCATTATTTTGAATGAT+TGG - chr4.4:34848778-34848797 MS.gene28968:intron 25.0%
!!! TTATCGATTTGTTGTTGATA+GGG - chr4.4:34848355-34848374 MS.gene28968:CDS 25.0%
!!! TTGTTTTGAATGTTTCAACT+GGG + chr4.4:34847577-34847596 None:intergenic 25.0%
!!! TTTATCGATTTGTTGTTGAT+AGG - chr4.4:34848354-34848373 MS.gene28968:CDS 25.0%
!!! TTTGTTTTGAATGTTTCAAC+TGG + chr4.4:34847578-34847597 None:intergenic 25.0%
AAACATGTCAAAACTAGTTC+TGG + chr4.4:34849728-34849747 None:intergenic 30.0%
AACACACAAAACACTATAGA+CGG - chr4.4:34850117-34850136 MS.gene28968:intron 30.0%
AAGTCATAAGAAAAAGGTTC+AGG + chr4.4:34848753-34848772 None:intergenic 30.0%
AGATAACTTTGTCTGATGAT+TGG - chr4.4:34846821-34846840 MS.gene28968:CDS 30.0%
AGTACTTATGAACAATCTAC+TGG - chr4.4:34848810-34848829 MS.gene28968:intron 30.0%
AGTCATAAGAAAAAGGTTCA+GGG + chr4.4:34848752-34848771 None:intergenic 30.0%
ATCCATCTAATTATTTCCTC+TGG + chr4.4:34848870-34848889 None:intergenic 30.0%
CACGACAATGTTGTAAAAAT+CGG - chr4.4:34849278-34849297 MS.gene28968:intron 30.0%
GACATGTTTGTAATAATGCA+GGG - chr4.4:34849739-34849758 MS.gene28968:intron 30.0%
GATAACTTTGTCTGATGATT+GGG - chr4.4:34846822-34846841 MS.gene28968:CDS 30.0%
GATTGATTAAACCTGAAATC+TGG + chr4.4:34847377-34847396 None:intergenic 30.0%
GCACAATATTCTTCATAATG+GGG + chr4.4:34850503-34850522 None:intergenic 30.0%
GCACTTAAAGAGAATATAAC+TGG - chr4.4:34850201-34850220 MS.gene28968:intron 30.0%
GGCACAATATTCTTCATAAT+GGG + chr4.4:34850504-34850523 None:intergenic 30.0%
GGTTAAATTGTGGTAATAGT+CGG + chr4.4:34849678-34849697 None:intergenic 30.0%
GTTAAATTGTGGTAATAGTC+GGG + chr4.4:34849677-34849696 None:intergenic 30.0%
TGACATGTTTGTAATAATGC+AGG - chr4.4:34849738-34849757 MS.gene28968:intron 30.0%
TGGCACAATATTCTTCATAA+TGG + chr4.4:34850505-34850524 None:intergenic 30.0%
TTTAAGTGCAACAAGATAAG+AGG + chr4.4:34850190-34850209 None:intergenic 30.0%
! AAAGTGGGACAAATCAATTT+TGG - chr4.4:34848214-34848233 MS.gene28968:CDS 30.0%
! AAATATGTTTTCCATCTGCA+GGG + chr4.4:34847291-34847310 None:intergenic 30.0%
! AGTGAAGAACGTTTTCTTTA+TGG - chr4.4:34847019-34847038 MS.gene28968:CDS 30.0%
! AGTTTTCGTCAAAATCTTAG+TGG - chr4.4:34848482-34848501 MS.gene28968:intron 30.0%
! ATCAAACAAGTGAGACTTTT+GGG + chr4.4:34849547-34849566 None:intergenic 30.0%
! ATCTTCATGGACATGATTTT+TGG - chr4.4:34850412-34850431 MS.gene28968:CDS 30.0%
! ATGAATTTTACAACCTCCTT+GGG - chr4.4:34846941-34846960 MS.gene28968:CDS 30.0%
! CATGGTTCAATTGGTTTAAT+CGG + chr4.4:34849523-34849542 None:intergenic 30.0%
! GAGGTTTGATTGTGTAATTT+TGG + chr4.4:34850065-34850084 None:intergenic 30.0%
! TAAATATGTTTTCCATCTGC+AGG + chr4.4:34847292-34847311 None:intergenic 30.0%
! TATGAATTTTACAACCTCCT+TGG - chr4.4:34846940-34846959 MS.gene28968:CDS 30.0%
! TCTTCATGGACATGATTTTT+GGG - chr4.4:34850413-34850432 MS.gene28968:CDS 30.0%
! TTCATTTTCTTCTACTTGAG+TGG - chr4.4:34847095-34847114 MS.gene28968:CDS 30.0%
! TTTTTATAAAGAAGCCAGAC+TGG + chr4.4:34849419-34849438 None:intergenic 30.0%
!!! TGTTTTTTATTTGCTCGAGT+TGG + chr4.4:34846888-34846907 None:intergenic 30.0%
AAAGATGAATGAATTCGTGC+AGG - chr4.4:34848394-34848413 MS.gene28968:intron 35.0%
AAGGGAACACATCAATGATA+TGG - chr4.4:34847909-34847928 MS.gene28968:intron 35.0%
AATGACTACGACTACCATTA+AGG - chr4.4:34849606-34849625 MS.gene28968:intron 35.0%
ACCAGGCTGTAACATTAAAT+AGG + chr4.4:34848652-34848671 None:intergenic 35.0%
ACCTATTTAATGTTACAGCC+TGG - chr4.4:34848648-34848667 MS.gene28968:CDS 35.0%
ACTCCATAATGATCAACAAG+TGG - chr4.4:34846798-34846817 MS.gene28968:CDS 35.0%
ACTGAAGTTCAATACAACTG+TGG - chr4.4:34850329-34850348 MS.gene28968:intron 35.0%
AGTGAAAACAAACTCTCGAT+AGG - chr4.4:34848088-34848107 MS.gene28968:intron 35.0%
CAAAACTATGACTCTATCTG+AGG + chr4.4:34850094-34850113 None:intergenic 35.0%
CATGTTATCGTTAAATCCAG+CGG + chr4.4:34846911-34846930 None:intergenic 35.0%
GACCAGAGGAAATAATTAGA+TGG - chr4.4:34848865-34848884 MS.gene28968:intron 35.0%
GATAAGTTACAGAATACCCA+AGG + chr4.4:34846960-34846979 None:intergenic 35.0%
GCATGATAAAGATATGTACC+AGG + chr4.4:34848669-34848688 None:intergenic 35.0%
TAAAAAAACATTAGGCGCGT+AGG + chr4.4:34847540-34847559 None:intergenic 35.0%
TAACTTATCCCACATACACA+AGG - chr4.4:34846971-34846990 MS.gene28968:CDS 35.0%
TAGAGATTCTTACCCAGAAA+AGG + chr4.4:34849808-34849827 None:intergenic 35.0%
TAGTTGAAAATGCCTAAACC+AGG + chr4.4:34849937-34849956 None:intergenic 35.0%
TATCTTTATCATGCTCACCA+TGG - chr4.4:34848675-34848694 MS.gene28968:CDS 35.0%
TCACTTCTGATTCATGGAAA+AGG - chr4.4:34848995-34849014 MS.gene28968:intron 35.0%
TCTGTGATAGTAGATTGATG+TGG + chr4.4:34849587-34849606 None:intergenic 35.0%
TGATCCAGTAGTTTCTTGAT+GGG + chr4.4:34849879-34849898 None:intergenic 35.0%
TTAAAATCATAGCCATCAGG+TGG + chr4.4:34850246-34850265 None:intergenic 35.0%
! ACAAATGCTACTTCTAGCAA+TGG - chr4.4:34850297-34850316 MS.gene28968:intron 35.0%
! ACACTATGTTGATCCTTTTC+TGG - chr4.4:34849792-34849811 MS.gene28968:intron 35.0%
! CACTATGTTGATCCTTTTCT+GGG - chr4.4:34849793-34849812 MS.gene28968:intron 35.0%
! GAACCACAATTTAGGGTTTA+GGG - chr4.4:34849486-34849505 MS.gene28968:intron 35.0%
! GATCAAACAAGTGAGACTTT+TGG + chr4.4:34849548-34849567 None:intergenic 35.0%
! GTCCCGTGAAATTTTTGATA+TGG + chr4.4:34847860-34847879 None:intergenic 35.0%
! GTCTGCAATCCCTTTTAAAT+GGG - chr4.4:34848836-34848855 MS.gene28968:intron 35.0%
! TCTCCACTTGTTGATCATTA+TGG + chr4.4:34846804-34846823 None:intergenic 35.0%
! TGAACCACAATTTAGGGTTT+AGG - chr4.4:34849485-34849504 MS.gene28968:intron 35.0%
! TGCTACCATGTTCACTATTT+TGG - chr4.4:34847419-34847438 MS.gene28968:CDS 35.0%
! TGTCTGCAATCCCTTTTAAA+TGG - chr4.4:34848835-34848854 MS.gene28968:intron 35.0%
!! GGTTTGTATGCATGCTATTT+TGG - chr4.4:34847339-34847358 MS.gene28968:CDS 35.0%
!!! ATTGGAACTCCTTGGTTTTA+TGG - chr4.4:34848284-34848303 MS.gene28968:CDS 35.0%
!!! CTTGGTTTTATGGAACAGAA+GGG - chr4.4:34848294-34848313 MS.gene28968:CDS 35.0%
!!! GATTTTTGGGTACTTGGATA+TGG - chr4.4:34850426-34850445 MS.gene28968:CDS 35.0%
!!! TGTATGCATGCTATTTTGGT+AGG - chr4.4:34847343-34847362 MS.gene28968:CDS 35.0%
!!! TTGGTTTTATGGAACAGAAG+GGG - chr4.4:34848295-34848314 MS.gene28968:CDS 35.0%
AAATTTCACGGGACTATCAG+TGG - chr4.4:34847866-34847885 MS.gene28968:intron 40.0%
AAGACCCATCAAGAAACTAC+TGG - chr4.4:34849872-34849891 MS.gene28968:intron 40.0%
AAGTTACAGAATACCCAAGG+AGG + chr4.4:34846957-34846976 None:intergenic 40.0%
AGAATCGTATGTGACTGATG+TGG + chr4.4:34849632-34849651 None:intergenic 40.0%
AGCACTCAGTTTCCAAATAG+AGG - chr4.4:34849156-34849175 MS.gene28968:intron 40.0%
AGTCTCACTTGTTTGATCAC+CGG - chr4.4:34849550-34849569 MS.gene28968:intron 40.0%
ATAATAGTGAGACGCATCCA+TGG - chr4.4:34850388-34850407 MS.gene28968:CDS 40.0%
ATGACTGTTGTTGAAGCAGA+TGG - chr4.4:34849769-34849788 MS.gene28968:intron 40.0%
ATTATTGTAAGCCCTGCAGA+TGG - chr4.4:34847277-34847296 MS.gene28968:CDS 40.0%
CATGAATCAGAAGTGACTGA+GGG + chr4.4:34848991-34849010 None:intergenic 40.0%
CCATAATTTCGTCAAGCTCA+CGG + chr4.4:34848430-34848449 None:intergenic 40.0%
CCTTCTGTTCCATAAAACCA+AGG + chr4.4:34848296-34848315 None:intergenic 40.0%
CGCTGGATTTAACGATAACA+TGG - chr4.4:34846909-34846928 MS.gene28968:CDS 40.0%
CGTGCATACATCAAAAACCA+TGG + chr4.4:34849262-34849281 None:intergenic 40.0%
GAAAAAGGTTCAGGGTCATT+AGG + chr4.4:34848744-34848763 None:intergenic 40.0%
GAGACGACTGTTTGTGAAAA+GGG + chr4.4:34849091-34849110 None:intergenic 40.0%
GAGCAAATAAAAAACACCGC+TGG - chr4.4:34846892-34846911 MS.gene28968:CDS 40.0%
GCTGTCCAAAATAGTGAACA+TGG + chr4.4:34847427-34847446 None:intergenic 40.0%
GGTATATGGAATGATTCGTG+TGG - chr4.4:34848716-34848735 MS.gene28968:intron 40.0%
GTGATCCAGTAGTTTCTTGA+TGG + chr4.4:34849880-34849899 None:intergenic 40.0%
GTTTCTGCTGACAACATTTG+AGG + chr4.4:34849902-34849921 None:intergenic 40.0%
TAAACCTGAAATCTGGTGTC+TGG + chr4.4:34847370-34847389 None:intergenic 40.0%
TAATCTCAGTTGAGACACCA+TGG - chr4.4:34849242-34849261 MS.gene28968:intron 40.0%
TCTCAATGTCCTATACTACC+TGG - chr4.4:34848320-34848339 MS.gene28968:CDS 40.0%
TCTTCTACTTGAGTGGAACA+AGG - chr4.4:34847102-34847121 MS.gene28968:CDS 40.0%
TGACCCTAAACCCTAAATTG+TGG + chr4.4:34849492-34849511 None:intergenic 40.0%
TGAGACGACTGTTTGTGAAA+AGG + chr4.4:34849092-34849111 None:intergenic 40.0%
TGATGTGGTCAAAACTAGAC+CGG + chr4.4:34849572-34849591 None:intergenic 40.0%
TGGGTACTTGGATATGGAAA+TGG - chr4.4:34850432-34850451 MS.gene28968:CDS 40.0%
TGTTTGATCAACGGTGAGTT+TGG + chr4.4:34848523-34848542 None:intergenic 40.0%
TTGAAAATGCCTAAACCAGG+CGG + chr4.4:34849934-34849953 None:intergenic 40.0%
! AATCCCTTTTAAATGGGTGG+GGG - chr4.4:34848842-34848861 MS.gene28968:intron 40.0%
! AGCAGTGGATTTGTCAGTTA+GGG + chr4.4:34847500-34847519 None:intergenic 40.0%
! ATGACTCTATCTGAGGTTGT+TGG + chr4.4:34850087-34850106 None:intergenic 40.0%
! CAATCCCTTTTAAATGGGTG+GGG - chr4.4:34848841-34848860 MS.gene28968:intron 40.0%
! CAATTTAGGGTTTAGGGTCA+TGG - chr4.4:34849492-34849511 MS.gene28968:intron 40.0%
! CACAATTGATTCAACCAGTC+TGG - chr4.4:34849402-34849421 MS.gene28968:intron 40.0%
! CAGACTGGTTGAATCAATTG+TGG + chr4.4:34849404-34849423 None:intergenic 40.0%
! GCAATCCCTTTTAAATGGGT+GGG - chr4.4:34848840-34848859 MS.gene28968:intron 40.0%
! TAGCAGTGGATTTGTCAGTT+AGG + chr4.4:34847501-34847520 None:intergenic 40.0%
! TGCAATCCCTTTTAAATGGG+TGG - chr4.4:34848839-34848858 MS.gene28968:intron 40.0%
! TTTGGTGAGCTTTGATTGCA+AGG + chr4.4:34850047-34850066 None:intergenic 40.0%
! TTTTCACAAACAGTCGTCTC+AGG - chr4.4:34849091-34849110 MS.gene28968:intron 40.0%
!! AATGCAGATTTGCTGCTTCA+GGG + chr4.4:34847465-34847484 None:intergenic 40.0%
!! ACAGTTCAACCTGGTTCTAT+CGG + chr4.4:34849374-34849393 None:intergenic 40.0%
!! CTTTTGGGTCATGGTTCAAT+TGG + chr4.4:34849532-34849551 None:intergenic 40.0%
!!! CCTTGGTTTTATGGAACAGA+AGG - chr4.4:34848293-34848312 MS.gene28968:CDS 40.0%
!!! GGACATGATTTTTGGGTACT+TGG - chr4.4:34850420-34850439 MS.gene28968:CDS 40.0%
AAAATGGTAGGGGATAGCAG+TGG + chr4.4:34847515-34847534 None:intergenic 45.0%
AAATCATAGCCATCAGGTGG+AGG + chr4.4:34850243-34850262 None:intergenic 45.0%
ACATCGTGTCCTTGTGTATG+TGG + chr4.4:34846983-34847002 None:intergenic 45.0%
ACTACCCAAACCATCCAATG+AGG - chr4.4:34849956-34849975 MS.gene28968:intron 45.0%
AGGTTTCCAGTGAAACCAGT+CGG + chr4.4:34849305-34849324 None:intergenic 45.0%
AGGTTTCGATCGGATAATCC+TGG - chr4.4:34850552-34850571 MS.gene28968:CDS 45.0%
ATGCTCACCATGGAATGCAA+AGG - chr4.4:34848685-34848704 MS.gene28968:CDS 45.0%
ATGGAATGCAAAGGGAAGCA+GGG - chr4.4:34848694-34848713 MS.gene28968:intron 45.0%
ATGTAGGTGCAAGCTCAACA+AGG - chr4.4:34847890-34847909 MS.gene28968:intron 45.0%
ATTGTGCCAGTGCACTCATT+TGG - chr4.4:34850516-34850535 MS.gene28968:CDS 45.0%
CATCGTGTCCTTGTGTATGT+GGG + chr4.4:34846982-34847001 None:intergenic 45.0%
CATTTGGTTGGACTGCTTTG+AGG - chr4.4:34850532-34850551 MS.gene28968:CDS 45.0%
CCATGAATCAGAAGTGACTG+AGG + chr4.4:34848992-34849011 None:intergenic 45.0%
CCGTGAGCTTGACGAAATTA+TGG - chr4.4:34848427-34848446 MS.gene28968:intron 45.0%
CCTCAGTCACTTCTGATTCA+TGG - chr4.4:34848989-34849008 MS.gene28968:intron 45.0%
CGGTGAGTTTGGCAAAATCA+TGG + chr4.4:34848512-34848531 None:intergenic 45.0%
CTATAGACGGTGTTCGTCAT+TGG - chr4.4:34850130-34850149 MS.gene28968:intron 45.0%
GATGAGTTACAAACACCAGC+AGG + chr4.4:34849052-34849071 None:intergenic 45.0%
GGATTTGTCAGTTAGGGTAC+TGG + chr4.4:34847494-34847513 None:intergenic 45.0%
GTTGTAAAAATCGGACCGAC+TGG - chr4.4:34849287-34849306 MS.gene28968:intron 45.0%
TACACAAGGACACGATGTTC+CGG - chr4.4:34846985-34847004 MS.gene28968:CDS 45.0%
TCATGTCCATGAAGATGCCA+TGG + chr4.4:34850408-34850427 None:intergenic 45.0%
TCTTCACTTAGCCTCACTAC+CGG + chr4.4:34847007-34847026 None:intergenic 45.0%
TGAAAATGCCTAAACCAGGC+GGG + chr4.4:34849933-34849952 None:intergenic 45.0%
TGCTCACCATGGAATGCAAA+GGG - chr4.4:34848686-34848705 MS.gene28968:CDS 45.0%
TGTAGGTGCAAGCTCAACAA+GGG - chr4.4:34847891-34847910 MS.gene28968:intron 45.0%
! AAGGTGTCTCCAGGTAGTAT+AGG + chr4.4:34848332-34848351 None:intergenic 45.0%
! ACTCTATCTGAGGTTGTTGG+AGG + chr4.4:34850084-34850103 None:intergenic 45.0%
! ATCACAGAGGAGCATGTTTG+TGG + chr4.4:34847611-34847630 None:intergenic 45.0%
! ATCCCTTTTAAATGGGTGGG+GGG - chr4.4:34848843-34848862 MS.gene28968:intron 45.0%
! CAAGATAAGAGGTGTGAGGT+AGG + chr4.4:34850179-34850198 None:intergenic 45.0%
! GATAGAACCAGGTTGAACTG+TGG - chr4.4:34849373-34849392 MS.gene28968:intron 45.0%
! GCAACAAGATAAGAGGTGTG+AGG + chr4.4:34850183-34850202 None:intergenic 45.0%
! TGTGACTGATGTGGCCTTAA+TGG + chr4.4:34849623-34849642 None:intergenic 45.0%
!! CAAGTGAGACTTTTGGGTCA+TGG + chr4.4:34849541-34849560 None:intergenic 45.0%
!! CAGTTCAACCTGGTTCTATC+GGG + chr4.4:34849373-34849392 None:intergenic 45.0%
!! CGATAAACAAAGGTGTCTCC+AGG + chr4.4:34848341-34848360 None:intergenic 45.0%
!! GAATGCAGATTTGCTGCTTC+AGG + chr4.4:34847466-34847485 None:intergenic 45.0%
!! GACTGCTTTGAGGTTTCGAT+CGG - chr4.4:34850542-34850561 MS.gene28968:CDS 45.0%
!! GTCAGTTAGGGTACTGGAAA+TGG + chr4.4:34847488-34847507 None:intergenic 45.0%
!! TGTGAAAAGGGAGAGCATTC+AGG + chr4.4:34849079-34849098 None:intergenic 45.0%
AAACCTGCCGGTGTAACACA+TGG - chr4.4:34849319-34849338 MS.gene28968:intron 50.0%
AACCAAACACCTCCACCTGA+TGG - chr4.4:34850231-34850250 MS.gene28968:intron 50.0%
ACGCATCCATGGCATCTTCA+TGG - chr4.4:34850399-34850418 MS.gene28968:CDS 50.0%
AGGTCCAGACACCAGATTTC+AGG - chr4.4:34847363-34847382 MS.gene28968:CDS 50.0%
ATTAGGCGCGTAGGCGAAAA+TGG + chr4.4:34847531-34847550 None:intergenic 50.0%
CACGACAGATTGGAACTCCT+TGG - chr4.4:34848276-34848295 MS.gene28968:CDS 50.0%
CATGGAATGCAAAGGGAAGC+AGG - chr4.4:34848693-34848712 MS.gene28968:intron 50.0%
CTGCTTCCCTTTGCATTCCA+TGG + chr4.4:34848695-34848714 None:intergenic 50.0%
CTGTGAGTGTGAACTGTCTG+CGG + chr4.4:34849350-34849369 None:intergenic 50.0%
GAAAATGCCTAAACCAGGCG+GGG + chr4.4:34849932-34849951 None:intergenic 50.0%
GACTCAACATTGTCCCAAGC+TGG + chr4.4:34850009-34850028 None:intergenic 50.0%
GCGACCTCATTGGATGGTTT+GGG + chr4.4:34849963-34849982 None:intergenic 50.0%
GGCTGAACCATGTGTTACAC+CGG + chr4.4:34849329-34849348 None:intergenic 50.0%
GGTTGAACCACAGTTCAACC+TGG + chr4.4:34849383-34849402 None:intergenic 50.0%
GTCCAACCAAATGAGTGCAC+TGG + chr4.4:34850525-34850544 None:intergenic 50.0%
GTCCCCCCACCCATTTAAAA+GGG + chr4.4:34848848-34848867 None:intergenic 50.0%
TCAACATTGTCCCAAGCTGG+TGG + chr4.4:34850006-34850025 None:intergenic 50.0%
TGCCAGTGCACTCATTTGGT+TGG - chr4.4:34850520-34850539 MS.gene28968:CDS 50.0%
TGGAATGCAAAGGGAAGCAG+GGG - chr4.4:34848695-34848714 MS.gene28968:intron 50.0%
TGGTTTCACTGGAAACCTGC+CGG - chr4.4:34849307-34849326 MS.gene28968:intron 50.0%
TTATCACCAAGCGTACCTGC+TGG - chr4.4:34849034-34849053 MS.gene28968:CDS 50.0%
! CAAAGGGAAGCAGGGGTATA+TGG - chr4.4:34848702-34848721 MS.gene28968:intron 50.0%
! GTGGTTTGTGCACGACAGAT+TGG - chr4.4:34848266-34848285 MS.gene28968:CDS 50.0%
ACATTGTCCCAAGCTGGTGG+AGG + chr4.4:34850003-34850022 None:intergenic 55.0%
ACTCACAGCCCGATAGAACC+AGG - chr4.4:34849362-34849381 MS.gene28968:intron 55.0%
AGAAACAGAACAACCCCGCC+TGG - chr4.4:34849916-34849935 MS.gene28968:intron 55.0%
AGAACAACCCCGCCTGGTTT+AGG - chr4.4:34849922-34849941 MS.gene28968:intron 55.0%
CAAACACCAGCAGGTACGCT+TGG + chr4.4:34849043-34849062 None:intergenic 55.0%
CGCGTAGGCGAAAATGGTAG+GGG + chr4.4:34847525-34847544 None:intergenic 55.0%
CGGGACTATCAGTGGCATGT+AGG - chr4.4:34847874-34847893 MS.gene28968:intron 55.0%
GAACCATGTGTTACACCGGC+AGG + chr4.4:34849325-34849344 None:intergenic 55.0%
GACACGATGTTCCGGTAGTG+AGG - chr4.4:34846993-34847012 MS.gene28968:CDS 55.0%
GCGCGTAGGCGAAAATGGTA+GGG + chr4.4:34847526-34847545 None:intergenic 55.0%
GGCGACCTCATTGGATGGTT+TGG + chr4.4:34849964-34849983 None:intergenic 55.0%
GGTCCCCCCACCCATTTAAA+AGG + chr4.4:34848849-34848868 None:intergenic 55.0%
!! AGCCATCAGGTGGAGGTGTT+TGG + chr4.4:34850236-34850255 None:intergenic 55.0%
!! ATCGGACCGACTGGTTTCAC+TGG - chr4.4:34849296-34849315 MS.gene28968:intron 55.0%
!! GATGTTGGTGGCGACCTCAT+TGG + chr4.4:34849973-34849992 None:intergenic 55.0%
!! TTGGTGGCGACCTCATTGGA+TGG + chr4.4:34849969-34849988 None:intergenic 55.0%
GGCGCGTAGGCGAAAATGGT+AGG + chr4.4:34847527-34847546 None:intergenic 60.0%
TAAATGGGTGGGGGGACCAG+AGG - chr4.4:34848851-34848870 MS.gene28968:intron 60.0%
TTGTCCCAAGCTGGTGGAGG+TGG + chr4.4:34850000-34850019 None:intergenic 60.0%
TCCCAAGCTGGTGGAGGTGG+CGG + chr4.4:34849997-34850016 None:intergenic 65.0%
TCCGCCACCTCCACCAGCTT+GGG - chr4.4:34849993-34850012 MS.gene28968:intron 65.0%
!! GGAGGTGGCGGAGATGTTGG+TGG + chr4.4:34849985-34850004 None:intergenic 65.0%
!! GGTGGAGGTGGCGGAGATGT+TGG + chr4.4:34849988-34850007 None:intergenic 65.0%
CTCCGCCACCTCCACCAGCT+TGG - chr4.4:34849992-34850011 MS.gene28968:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 34846793 34850581 34846793 ID=MS.gene28968
chr4.4 mRNA 34846793 34850581 34846793 ID=MS.gene28968.t1;Parent=MS.gene28968
chr4.4 exon 34850365 34850581 34850365 ID=MS.gene28968.t1.exon1;Parent=MS.gene28968.t1
chr4.4 CDS 34850365 34850581 34850365 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
chr4.4 exon 34850014 34850114 34850014 ID=MS.gene28968.t1.exon2;Parent=MS.gene28968.t1
chr4.4 CDS 34850014 34850114 34850014 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
chr4.4 exon 34848998 34849090 34848998 ID=MS.gene28968.t1.exon3;Parent=MS.gene28968.t1
chr4.4 CDS 34848998 34849090 34848998 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
chr4.4 exon 34848517 34848708 34848517 ID=MS.gene28968.t1.exon4;Parent=MS.gene28968.t1
chr4.4 CDS 34848517 34848708 34848517 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
chr4.4 exon 34848197 34848379 34848197 ID=MS.gene28968.t1.exon5;Parent=MS.gene28968.t1
chr4.4 CDS 34848197 34848379 34848197 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
chr4.4 exon 34847560 34847614 34847560 ID=MS.gene28968.t1.exon6;Parent=MS.gene28968.t1
chr4.4 CDS 34847560 34847614 34847560 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
chr4.4 exon 34846793 34847535 34846793 ID=MS.gene28968.t1.exon7;Parent=MS.gene28968.t1
chr4.4 CDS 34846793 34847535 34846793 ID=cds.MS.gene28968.t1;Parent=MS.gene28968.t1
Gene Sequence

>MS.gene28968

ATGAAACTCCATAATGATCAACAAGTGGAGATAACTTTGTCTGATGATTGGGAAAAAGTGAAAATTCAACATGAGAGAAAAATGGCTCGACTCCAACTCGAGCAAATAAAAAACACCGCTGGATTTAACGATAACATGGATGCTATGTATGAATTTTACAACCTCCTTGGGTATTCTGTAACTTATCCCACATACACAAGGACACGATGTTCCGGTAGTGAGGCTAAGTGAAGAACGTTTTCTTTATGGTTCTTGAGATATTGAGATGAAGTGACATTTCTCAGTATAGATTATCTTCCATTTTCATTTTCTTCTACTTGAGTGGAACAAGGATTAATCAAAACATTCTTGTATTTTTTTCAATTAAAATGTGAAATGGTTATTTCTATTGTTTCTTTCGTTTCATTAATTTTACTGGTCTCTTATATTATTATCATTATTTTATCAGGTGAGTCAATCATCTTCAGCTCAAACTATCTACAACATTATTGTAAGCCCTGCAGATGGAAAACATATTTATCCTATTAATCTTCTAAATACTAACTGGGTTTGTATGCATGCTATTTTGGTAGGTCCAGACACCAGATTTCAGGTTTAATCAATCTATATGTTTACTAACTAACTACTGCTACCATGTTCACTATTTTGGACAGCATGTATGCTCATGAACCCTGAAGCAGCAAATCTGCATTCCATTTCCAGTACCCTAACTGACAAATCCACTGCTATCCCCTACCATTTTCGCCTACGCGCCTAATGTTTTTTTATAAGCATTGACATGCCCAGTTGAAACATTCAAAACAAATCTACTTCTACCACAAACATGCTCCTCTGTGATTGTAAATAATAGTGCAGACATTAATCACATGTTCTTATATTCGTTAATTGATTAACTAAACTGGTGTTTCCACTTTTTCATAACATGATACAAGTGCGACAAGACACAAGAATGACCCATTTCATATAACAAAAGATGAAACTTCATTTTTTTCACTTGCCTTTGTAGTTATTACTACATTATCAGTAATTAATTACATAGTAACATATGACCTAAAAGTCATATTCCATATCAAAAATTTCACGGGACTATCAGTGGCATGTAGGTGCAAGCTCAACAAGGGAACACATCAATGATATGGTCACATTCAAGAAATACATGTATGCATAATACATCAATATATGCTTAAGAAAGACTTTACGGAGTCCTTGACAAATGCATAAAGAAAAGGATATACAACTCTATGAACATACTTCTCCCTAATAGATTTTAGAAGTAAATGCAAGGAAGTTTCTAAAGTGAAAACAAACTCTCGATAGGATAGTCAGTCAGCGCAAATAATTTCAAACAGTCAAAGCGTCAATCAGAGCTAGAAAATACATATATACACATCATAATATATCAGATATTTGTTTTAAACTACAAAGTGGGACAAATCAATTTTGGTGCTTAATGATTAATATATAATGACTGATGTGGTTTGTGCACGACAGATTGGAACTCCTTGGTTTTATGGAACAGAAGGGGTGTCTCAATGTCCTATACTACCTGGAGACACCTTTGTTTATCGATTTGTTGTTGATAGGGTAAGTTGTCAAAATGAAAAGATGAATGAATTCGTGCAGGTTTAGACTCACCGTGAGCTTGACGAAATTATGGTAGCACCATGATTTTATTGAAGCTTTAAGTGTAGTTTTCGTCAAAATCTTAGTGGCACACCATGATTTTGCCAAACTCACCGTTGATCAAACATGATCTATTCAATATTCATTGATATGTTGCTCTTCATTTCAACACTAACAAACTATATTTATATCATCAATCATAGCGTCAAAATTGTTAATATCTATTTTGGTTACCTATTTAATGTTACAGCCTGGTACATATCTTTATCATGCTCACCATGGAATGCAAAGGGAAGCAGGGGTATATGGAATGATTCGTGTGGCACCTAATGACCCTGAACCTTTTTCTTATGACTTTGATAGAAGCATTATTTTGAATGATTGGTACCATAAGAGTACTTATGAACAATCTACTGGATTGTCTGCAATCCCTTTTAAATGGGTGGGGGGACCAGAGGAAATAATTAGATGGATATACTAATCAGAAAAATACAAATTCAAAATCTTATATCTAATTTTCTTTCTTCAATTTTCTAATTGCTGGTTTTATTGTTTCTTTGTATATTTGGTTTCCCTCAGTCACTTCTGATTCATGGAAAAGGAAGATACAACTGTTCGTTATCACCAAGCGTACCTGCTGGTGTTTGTAACTCATCAAATCCTGAATGCTCTCCCTTTTCACAAACAGTCGTCTCAGGAAAAACATACAGAATTAGAGTTGCAAGCTTGACTGCTTTATCAGCACTCAGTTTCCAAATAGAGGTAATCATTCATTCACTCAATTATTCTAAGTATCATTCAAGTTTTTAAACAAGGTCTGCGACAATAATCTCAGTTGAGACACCATGGTTTTTGATGTATGCACGACAATGTTGTAAAAATCGGACCGACTGGTTTCACTGGAAACCTGCCGGTGTAACACATGGTTCAGCCGCAGACAGTTCACACTCACAGCCCGATAGAACCAGGTTGAACTGTGGTTCAACCACAATTGATTCAACCAGTCTGGCTTCTTTATAAAAAAATATACATTTTTAATGTCTATTTATTTATTTCTTAGTCATATTGTTGAACCACAATTTAGGGTTTAGGGTCATGGCTAAACCGATTAAACCAATTGAACCATGACCCAAAAGTCTCACTTGTTTGATCACCGGTCTAGTTTTGACCACATCAATCTACTATCACAGAATGACTACGACTACCATTAAGGCCACATCAGTCACATACGATTCTTTGCAATATCAAAAGATTGTGACCCGACTATTACCACAATTTAACCTTGTTTAATTTTTCTCACATTTTACAACCAGAACTAGTTTTGACATGTTTGTAATAATGCAGGGCAATAACATGACTGTTGTTGAAGCAGATGGACACTATGTTGATCCTTTTCTGGGTAAGAATCTCTACATATAATCAGGTGAAACATACTCAGTTCTAGTCAAAACTGATCAAGACCCATCAAGAAACTACTGGATCACCTCAAATGTTGTCAGCAGAAACAGAACAACCCCGCCTGGTTTAGGCATTTTCAACTACTACCCAAACCATCCAATGAGGTCGCCACCAACATCTCCGCCACCTCCACCAGCTTGGGACAATGTTGAGTCAAGACATACTCAAAGCCTTGCAATCAAAGCTCACCAAAATTACACAATCAAACCTCCAACAACCTCAGATAGAGTCATAGTTTTGCTCAACACACAAAACACTATAGACGGTGTTCGTCATTGGTCTGTTAACAACGTAGCATTTTTCCTACCTCACACCTCTTATCTTGTTGCACTTAAAGAGAATATAACTGGTGAATTCAACCAAACACCTCCACCTGATGGCTATGATTTTAATAATTATGATATTTTCAGTGTGGCTAACAACACAAATGCTACTTCTAGCAATGGAATTTATAGACTGAAGTTCAATACAACTGTGGATATTATACTACAAAATGCAAACACTATGAATAAAAATAATAGTGAGACGCATCCATGGCATCTTCATGGACATGATTTTTGGGTACTTGGATATGGAAATGGTAAATTTGATGTGAACAAAGACCCAAAAAATTATAACTTGGTGAACCCCATTATGAAGAATATTGTGCCAGTGCACTCATTTGGTTGGACTGCTTTGAGGTTTCGATCGGATAATCCTGGTGTGTAG
Protein sequence

>MS.gene28968.t1

MKLHNDQQVEITLSDDWEKVKIQHERKMARLQLEQIKNTAGFNDNMDAMYEFYNLLGYSVTYPTYTRTRCSGTQTIYNIIVSPADGKHIYPINLLNTNWVCMHAILIGTPWFYGTEGVSQCPILPGDTFVYRFVVDRPGTYLYHAHHGMQREAGVYGMIRVAPNDPEPFSYDFDRSIILNDWYHKSTYEQSTGLSAIPFKWSLLIHGKGRYNCSLSPSVPAGVCNSSNPECSPFSQTVVSGKTYRIRVASLTALSALSFQIEGNNMTVVEADGHYVDPFLGETYSVLVKTDQDPSRNYWITSNVVSRNRTTPPGLGIFNYYPNHPMRSPPTSPPPPPAWDNVESRHTQSLAIKAHQNYTIKPPTTSDRVIVLLNTQNTIDGVRHWSVNNVAFFLPHTSYLVALKENITGEFNQTPPPDGYDFNNYDIFSVANNTNATSSNGIYRLKFNTTVDIILQNANTMNKNNSETHPWHLHGHDFWVLGYGNGKFDVNKDPKNYNLVNPIMKNIVPVHSFGWTALRFRSDNPGV