AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene021147

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021147.t1 MTR_4g064530 97.445 587 13 1 1 587 1 585 0.0 1170
MS.gene021147.t1 MTR_4g064530 96.915 551 15 1 1 551 1 549 0.0 1083
MS.gene021147.t1 MTR_7g060460 73.540 582 142 6 14 587 4 581 0.0 890
MS.gene021147.t1 MTR_7g058690 72.355 586 147 6 11 587 1 580 0.0 874
MS.gene021147.t1 MTR_7g458880 70.578 588 158 5 7 587 1 580 0.0 868
MS.gene021147.t1 MTR_7g062250 70.486 576 157 5 16 587 12 578 0.0 863
MS.gene021147.t1 MTR_7g062310 66.966 557 175 4 37 587 40 593 0.0 785
MS.gene021147.t1 MTR_5g069680 54.827 549 232 7 41 587 24 558 0.0 625
MS.gene021147.t1 MTR_3g462760 54.562 548 234 5 41 587 23 556 0.0 624
MS.gene021147.t1 MTR_4g015120 54.862 545 234 5 44 587 24 557 0.0 622
MS.gene021147.t1 MTR_5g020600 53.370 549 241 6 41 587 31 566 0.0 619
MS.gene021147.t1 MTR_5g020620 51.736 576 260 8 14 587 2 561 0.0 612
MS.gene021147.t1 MTR_5g083360 51.127 577 261 10 20 587 3 567 0.0 592
MS.gene021147.t1 MTR_5g081810 52.222 540 243 5 49 587 4 529 0.0 586
MS.gene021147.t1 MTR_5g073210 49.656 582 263 12 13 587 2 560 0.0 546
MS.gene021147.t1 MTR_3g071890 48.592 568 247 12 20 564 13 558 0.0 525
MS.gene021147.t1 MTR_8g027375 46.998 583 278 11 21 587 4 571 4.99e-180 521
MS.gene021147.t1 MTR_2g008330 45.803 548 287 6 44 587 38 579 7.67e-180 520
MS.gene021147.t1 MTR_7g065980 44.621 567 297 8 31 587 12 571 1.64e-173 504
MS.gene021147.t1 MTR_7g065970 47.049 576 281 11 25 587 6 570 1.17e-169 494
MS.gene021147.t1 MTR_4g019225 45.144 556 282 7 45 587 27 572 7.90e-168 489
MS.gene021147.t1 MTR_3g101635 46.060 571 286 10 13 577 2 556 9.27e-167 486
MS.gene021147.t1 MTR_3g101640 44.940 583 297 12 13 587 5 571 1.14e-166 486
MS.gene021147.t1 MTR_8g091390 30.526 570 322 20 44 577 34 565 8.18e-65 223
MS.gene021147.t1 MTR_3g078700 31.028 564 313 19 45 587 27 535 1.48e-61 213
MS.gene021147.t1 MTR_5g081790 41.958 286 149 6 269 553 159 428 1.62e-61 210
MS.gene021147.t1 MTR_5g081790 51.136 176 73 2 19 187 5 174 2.55e-55 193
MS.gene021147.t1 MTR_3g078740 31.122 588 328 22 40 577 22 582 4.09e-60 210
MS.gene021147.t1 MTR_3g078730 30.700 557 314 21 44 577 25 532 1.66e-58 205
MS.gene021147.t1 MTR_4g101650 27.381 588 363 15 19 587 7 549 6.16e-48 177
MS.gene021147.t1 MTR_8g073840 44.330 194 69 4 37 229 23 178 9.96e-46 163
MS.gene021147.t1 MTR_3g008580 26.401 553 348 14 19 552 4 516 1.31e-45 170
MS.gene021147.t1 MTR_1g090707 28.807 545 327 19 58 587 38 536 4.96e-42 160
MS.gene021147.t1 MTR_7g058670 74.074 81 20 1 101 180 13 93 1.17e-38 137
MS.gene021147.t1 MTR_1g026410 24.908 546 366 14 44 587 29 532 2.31e-35 140
MS.gene021147.t1 MTR_7g051440 26.128 532 322 18 45 552 30 514 6.12e-35 139
MS.gene021147.t1 MTR_3g115050 23.498 566 349 14 44 587 30 533 1.24e-32 132
MS.gene021147.t1 MTR_4g049390 24.855 519 320 14 44 547 27 490 1.59e-31 129
MS.gene021147.t1 MTR_4g075690 23.878 557 357 15 44 587 30 532 1.74e-30 125
MS.gene021147.t1 MTR_3g083450 24.955 561 359 17 45 582 27 548 5.72e-29 121
MS.gene021147.t1 MTR_2g088080 24.478 527 312 14 44 547 30 493 8.20e-29 120
MS.gene021147.t1 MTR_3g083430 24.468 564 358 20 45 582 26 547 8.67e-29 120
MS.gene021147.t1 MTR_4g048350 24.808 520 318 17 44 547 27 489 1.20e-28 120
MS.gene021147.t1 MTR_5g018270 25.641 507 327 15 44 547 31 490 3.83e-28 119
MS.gene021147.t1 MTR_3g083390 24.515 567 354 18 45 582 26 547 4.18e-28 119
MS.gene021147.t1 MTR_8g479380 24.376 521 346 16 33 547 13 491 4.30e-28 119
MS.gene021147.t1 MTR_3g083400 24.339 567 355 18 45 582 26 547 6.92e-28 118
MS.gene021147.t1 MTR_3g083440 24.339 567 355 18 45 582 26 547 8.62e-28 118
MS.gene021147.t1 MTR_4g083210 24.717 530 340 16 45 563 26 507 4.02e-27 115
MS.gene021147.t1 MTR_8g479390 24.085 519 323 16 44 547 31 493 1.09e-26 114
MS.gene021147.t1 MTR_3g083410 24.497 547 339 18 65 582 10 511 7.22e-26 112
MS.gene021147.t1 MTR_4g075690 28.358 268 179 6 44 310 30 285 2.62e-25 109
MS.gene021147.t1 MTR_4g132320 24.706 510 332 16 44 547 31 494 3.60e-25 110
MS.gene021147.t1 MTR_4g083190 25.000 532 356 17 19 547 6 497 2.93e-23 104
MS.gene021147.t1 MTR_2g088080 23.871 465 284 14 98 547 3 412 3.12e-19 91.3
MS.gene021147.t1 MTR_2g436410 56.338 71 27 2 388 457 5 72 1.44e-15 73.6
MS.gene021147.t1 MTR_3g079660 39.080 87 46 2 497 578 5 89 5.51e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene021147.t1 AT2G29130 70.139 576 158 6 15 587 9 573 0.0 859
MS.gene021147.t1 AT5G60020 69.430 579 156 6 20 587 9 577 0.0 850
MS.gene021147.t1 AT5G60020 71.664 547 142 5 52 587 1 545 0.0 833
MS.gene021147.t1 AT5G60020 69.088 537 145 6 20 545 9 535 0.0 778
MS.gene021147.t1 AT2G38080 54.306 569 242 6 19 587 8 558 0.0 634
MS.gene021147.t1 AT5G58910 52.028 567 252 8 25 587 17 567 0.0 608
MS.gene021147.t1 AT5G03260 52.007 573 253 9 18 587 4 557 0.0 608
MS.gene021147.t1 AT5G58910 52.028 567 251 9 25 587 17 566 0.0 604
MS.gene021147.t1 AT2G40370 51.178 594 257 11 11 587 3 580 0.0 592
MS.gene021147.t1 AT5G05390 53.276 580 242 11 16 587 7 565 0.0 591
MS.gene021147.t1 AT5G01190 52.000 575 256 8 15 587 2 558 0.0 588
MS.gene021147.t1 AT1G18140 50.622 563 257 10 40 587 25 581 0.0 578
MS.gene021147.t1 AT2G30210 50.172 580 268 9 16 587 4 570 0.0 571
MS.gene021147.t1 AT5G58910 51.296 540 242 7 52 587 1 523 0.0 570
MS.gene021147.t1 AT5G01190 51.130 575 247 9 15 587 2 544 0.0 565
MS.gene021147.t1 AT5G07130 50.090 553 256 11 46 587 26 569 0.0 529
MS.gene021147.t1 AT2G46570 44.905 579 290 11 20 587 9 569 2.28e-180 521
MS.gene021147.t1 AT5G09360 44.406 572 292 12 22 587 18 569 3.92e-172 500
MS.gene021147.t1 AT3G09220 46.435 547 274 8 32 570 15 550 3.48e-171 498
MS.gene021147.t1 AT5G48100 45.505 545 277 11 42 577 20 553 2.98e-163 478
MS.gene021147.t1 AT5G07130 50.101 493 226 11 106 587 1 484 4.13e-158 461
MS.gene021147.t1 AT5G07130 50.101 493 226 11 106 587 1 484 4.13e-158 461
MS.gene021147.t1 AT5G01050 42.606 568 299 10 32 587 16 568 1.45e-154 456
MS.gene021147.t1 AT2G46570 44.711 501 249 10 98 587 1 484 5.48e-152 446
MS.gene021147.t1 AT5G01040 42.226 566 302 10 32 587 16 566 1.47e-151 448
MS.gene021147.t1 AT3G09220 47.619 462 224 7 117 570 2 453 1.79e-142 421
MS.gene021147.t1 AT5G21105 30.653 597 346 19 17 577 9 573 1.06e-68 233
MS.gene021147.t1 AT4G39830 30.619 565 317 18 44 570 36 563 1.12e-68 233
MS.gene021147.t1 AT5G21100 30.515 544 302 17 59 566 39 542 5.69e-67 228
MS.gene021147.t1 AT5G21105 30.851 564 321 21 17 545 9 538 6.35e-60 208
MS.gene021147.t1 AT4G39830 29.821 503 279 17 105 570 3 468 1.26e-49 179
MS.gene021147.t1 AT4G39830 29.920 498 275 17 110 570 6 466 3.41e-49 178
MS.gene021147.t1 AT4G12420 26.996 526 325 13 46 552 27 512 2.06e-46 172
MS.gene021147.t1 AT4G12420 26.996 526 325 13 46 552 27 512 2.06e-46 172
MS.gene021147.t1 AT4G39830 28.884 502 272 16 105 570 3 455 7.37e-45 166
MS.gene021147.t1 AT1G75790 27.509 578 369 19 20 587 4 541 4.25e-44 165
MS.gene021147.t1 AT5G51480 27.725 523 315 15 46 547 30 510 4.93e-44 166
MS.gene021147.t1 AT4G25240 26.644 593 359 17 15 580 1 544 1.46e-43 164
MS.gene021147.t1 AT4G25240 26.644 593 359 17 15 580 1 544 1.46e-43 164
MS.gene021147.t1 AT5G21105 32.033 359 208 10 228 577 51 382 1.37e-38 147
MS.gene021147.t1 AT1G21860 25.000 540 345 14 23 553 8 496 4.01e-37 145
MS.gene021147.t1 AT5G48450 26.731 520 340 17 46 561 31 513 1.46e-36 144
MS.gene021147.t1 AT1G21850 24.205 566 346 17 1 553 1 496 2.22e-36 143
MS.gene021147.t1 AT5G48450 26.731 520 340 17 46 561 31 513 2.30e-36 144
MS.gene021147.t1 AT1G76160 24.660 588 369 17 13 587 4 530 1.27e-32 132
MS.gene021147.t1 AT4G22010 25.312 561 346 20 44 587 26 530 4.32e-31 127
MS.gene021147.t1 AT1G41830 23.717 565 387 14 24 587 10 531 8.06e-31 127
MS.gene021147.t1 AT5G66920 23.883 515 336 14 44 551 37 502 1.32e-30 126
MS.gene021147.t1 AT3G13390 25.540 509 335 14 45 547 29 499 5.65e-27 115
MS.gene021147.t1 AT4G28090 22.587 549 380 13 43 587 27 534 8.45e-27 115
MS.gene021147.t1 AT4G37160 23.935 493 333 13 62 553 49 500 9.68e-27 114
MS.gene021147.t1 AT3G13400 25.000 504 343 14 45 547 28 497 1.94e-25 110
MS.gene021147.t1 AT3G13400 25.000 504 343 14 45 547 28 497 1.94e-25 110
MS.gene021147.t1 AT1G55570 24.852 507 341 15 45 547 30 500 2.09e-25 110
MS.gene021147.t1 AT4G38420 21.526 511 365 13 44 553 30 505 8.55e-25 108
MS.gene021147.t1 AT2G23630 25.462 487 320 14 62 547 48 492 7.22e-24 106
MS.gene021147.t1 AT2G23630 25.462 487 320 14 62 547 53 497 7.75e-24 105
MS.gene021147.t1 AT1G55560 29.455 275 183 7 62 335 44 308 1.74e-23 105
MS.gene021147.t1 AT4G38420 21.355 487 347 13 44 529 30 481 1.58e-20 95.5
MS.gene021147.t1 AT4G38420 26.087 276 193 6 44 318 30 295 1.69e-20 95.1
MS.gene021147.t1 AT4G37160 27.016 248 169 4 62 308 49 285 4.46e-20 93.6

Find 150 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCACATTTGCAGGGTATTT+TGG 0.173773 4.4:-41747258 None:intergenic
TCTGATGCCTACACCATTAA+TGG 0.176037 4.4:+41746181 MS.gene021147:CDS
ATTGCAGGATACAAGCATTT+TGG 0.187461 4.4:+41747596 MS.gene021147:CDS
TTTGAATTTGAACTATTATT+TGG 0.212461 4.4:-41747360 None:intergenic
TGCACCTTAGTTGGGGATTA+AGG 0.227331 4.4:+41748285 MS.gene021147:CDS
CTTCTCCAACAACATTTCTT+TGG 0.228697 4.4:+41747432 MS.gene021147:CDS
CATTTCTTTGGAACTAATAA+TGG 0.256030 4.4:+41747444 MS.gene021147:CDS
AACCTCCCCTGGATTTGTTT+TGG 0.259173 4.4:+41745003 MS.gene021147:CDS
TTTGTTGTTGGTCAAGGTTT+TGG 0.271917 4.4:+41747660 MS.gene021147:CDS
TAACTCTGACCTGTTTGAAT+TGG 0.297459 4.4:-41745729 None:intergenic
CTTTGATGTGCACCTTAGTT+GGG 0.307238 4.4:+41748277 MS.gene021147:CDS
AATACTGTTGCTGTGCCTTC+TGG 0.309543 4.4:+41747741 MS.gene021147:CDS
AATGTGGCATTTGGGTAATT+TGG 0.312994 4.4:-41747042 None:intergenic
ACGAGAAGGGGACAGATTAT+TGG 0.313664 4.4:+41745304 MS.gene021147:CDS
CAATGCAGGCTGATATTGTT+TGG 0.314456 4.4:-41745347 None:intergenic
TGCTGTGCCTTCTGGTGGTT+GGG 0.319801 4.4:+41747749 MS.gene021147:CDS
TACACCATTAATGGGTTTCC+TGG 0.319870 4.4:+41746190 MS.gene021147:CDS
TTGGTGTAGTAATCAACATT+TGG 0.322522 4.4:-41748375 None:intergenic
TGGTGTAGTAATCAACATTT+GGG 0.326399 4.4:-41748374 None:intergenic
AGTCATCCTTTGCATCTTCA+TGG 0.334535 4.4:+41747627 MS.gene021147:CDS
AAGGAAGTCCCCATTCTCTT+TGG 0.338186 4.4:+41745891 MS.gene021147:CDS
ACCTGAACTCTAGTGTTATA+TGG 0.345887 4.4:-41747570 None:intergenic
GCTTGTGCAATTACAGCTTC+AGG 0.346802 4.4:-41746130 None:intergenic
TGGTTAAGAGCTACTGTCTA+TGG 0.350969 4.4:+41745813 MS.gene021147:CDS
TTACTAACCAAAGTATTGTT+TGG 0.352391 4.4:-41747525 None:intergenic
GGATTTAATTTCTTTGTTGT+TGG 0.356145 4.4:+41747648 MS.gene021147:CDS
CTGATGCCTACACCATTAAT+GGG 0.371595 4.4:+41746182 MS.gene021147:CDS
TATTTGGTCCTTGACAAGTT+TGG 0.374143 4.4:-41747344 None:intergenic
TTGCTGTGCCTTCTGGTGGT+TGG 0.376214 4.4:+41747748 MS.gene021147:CDS
AGGTCCAGGAAACCCATTAA+TGG 0.377126 4.4:-41746194 None:intergenic
AACTCTGACCTGTTTGAATT+GGG 0.379719 4.4:-41745728 None:intergenic
AACAAAATTAGTATCATTGA+TGG 0.379750 4.4:-41747199 None:intergenic
CAACATTTGGGGAGATCCTT+AGG 0.380323 4.4:-41748362 None:intergenic
AGCATGTAGAATGTGGCATT+TGG 0.380488 4.4:-41747051 None:intergenic
GACAAAGAGGAACTCTCTTC+TGG 0.381182 4.4:+41745775 MS.gene021147:CDS
AAAAGCATGGTTACCGTGAA+TGG 0.383433 4.4:+41745254 MS.gene021147:CDS
AGGGGACAGATTATTGGTTA+AGG 0.383575 4.4:+41745310 MS.gene021147:CDS
TCAACAAGATTAAACCTTGC+AGG 0.384892 4.4:-41747705 None:intergenic
GTACCCTGCCCTGTGAAATA+TGG 0.385825 4.4:-41747081 None:intergenic
TAGAGTTCAGGTTATATTGC+AGG 0.393539 4.4:+41747581 MS.gene021147:CDS
TACCGTGAATGGGAAGTTCC+CGG 0.400570 4.4:+41745265 MS.gene021147:CDS
GAGAATGGGGACTTCCTTGT+AGG 0.406530 4.4:-41745886 None:intergenic
GTGTAGGCATCAGAAACATT+TGG 0.413299 4.4:-41746172 None:intergenic
GCATGTAGAATGTGGCATTT+GGG 0.416680 4.4:-41747050 None:intergenic
ACTTTGATGTGCACCTTAGT+TGG 0.416806 4.4:+41748276 MS.gene021147:CDS
ACCTTCCTCTTAACCTCCCC+TGG 0.418136 4.4:+41744992 MS.gene021147:CDS
ACCATATAACACTAGAGTTC+AGG 0.422189 4.4:+41747569 MS.gene021147:CDS
GTATAGTTAAACGGCCTTAA+CGG 0.423945 4.4:-41747492 None:intergenic
TTCGACAATTCCACAGTAGC+TGG 0.425573 4.4:+41747105 MS.gene021147:CDS
AATTTCTTTGTTGTTGGTCA+AGG 0.431250 4.4:+41747654 MS.gene021147:CDS
TTAAGGCCGTTTAACTATAC+AGG 0.432215 4.4:+41747495 MS.gene021147:CDS
AACAATACTTTGGTTAGTAA+TGG 0.432888 4.4:+41747528 MS.gene021147:CDS
GATAAGATTCATTGTGCCTT+GGG 0.433565 4.4:-41745851 None:intergenic
CAAATCCAGGGGAGGTTAAG+AGG 0.435761 4.4:-41744997 None:intergenic
GTGTACAACTTCACCATTGT+TGG 0.440870 4.4:+41745753 MS.gene021147:CDS
TTCATATGTACCAAAGAGAA+TGG 0.444243 4.4:-41745901 None:intergenic
AACGGCCTTAACGGTACAAC+CGG 0.445112 4.4:-41747483 None:intergenic
TGTAGGCATCAGAAACATTT+GGG 0.450843 4.4:-41746171 None:intergenic
GTTGGGGATTAAGGATGGCT+TGG 0.452720 4.4:+41748294 MS.gene021147:CDS
GCAACAGTATTCCTCTCAAC+AGG 0.458149 4.4:-41747729 None:intergenic
AACTCCTCCAAACAATACTT+TGG 0.459111 4.4:+41747518 MS.gene021147:CDS
GGATAAGATTCATTGTGCCT+TGG 0.462220 4.4:-41745852 None:intergenic
TCATTGATGGCTGGAAGCAC+AGG 0.471762 4.4:-41747186 None:intergenic
AAAATAGTTCTTACCAATGC+AGG 0.472186 4.4:-41745361 None:intergenic
GGAGTTCCTGTATAGTTAAA+CGG 0.473322 4.4:-41747501 None:intergenic
TCATATGTACCAAAGAGAAT+GGG 0.475158 4.4:-41745900 None:intergenic
TCTGGCATGCTCATTTCTCA+TGG 0.475847 4.4:+41745793 MS.gene021147:CDS
TAGTTCCAAAGAAATGTTGT+TGG 0.480695 4.4:-41747437 None:intergenic
ATTATTTCTGCAGATAAGGT+TGG 0.482733 4.4:+41745205 MS.gene021147:intron
ATTGCACAAGCTCTGCAGAC+AGG 0.483287 4.4:+41746142 MS.gene021147:CDS
ACTCAATGCCCAATTCAAAC+AGG 0.483829 4.4:+41745720 MS.gene021147:CDS
GAAGTAGCCAAAACAAATCC+AGG 0.492773 4.4:-41745010 None:intergenic
TTCACCATTGTTGGACAAAG+AGG 0.495747 4.4:+41745762 MS.gene021147:CDS
GGGCATTGAGTTATGTATGA+TGG 0.498399 4.4:-41745708 None:intergenic
CCTTCTCGTACAACAATTCG+AGG 0.499048 4.4:-41745290 None:intergenic
TGGTCCAAGTATGATGGTAT+TGG 0.499764 4.4:-41746986 None:intergenic
GTATGGTTGAAACAGGTGTT+TGG 0.506981 4.4:+41748240 MS.gene021147:intron
ACCCGGGAACTTCCCATTCA+CGG 0.507216 4.4:-41745267 None:intergenic
GAGCAATTGTAAAGAGGTCC+AGG 0.507787 4.4:-41746208 None:intergenic
CTTCGTAGTGGTTGGTCGGA+TGG 0.508027 4.4:+41745684 MS.gene021147:CDS
CGATACCTTGCTGACAATCC+AGG 0.508335 4.4:+41747780 MS.gene021147:CDS
CTCGAATTGTTGTACGAGAA+GGG 0.509000 4.4:+41745291 MS.gene021147:CDS
TGTTGGAGAAGTGCTATAGA+AGG 0.510179 4.4:-41747420 None:intergenic
CCTCTTTACAATTGCTCCAA+AGG 0.511152 4.4:+41746214 MS.gene021147:CDS
AAAGCATGGTTACCGTGAAT+GGG 0.513288 4.4:+41745255 MS.gene021147:CDS
AAGGATGGCTTGGATTGTTG+AGG 0.513739 4.4:+41748304 MS.gene021147:CDS
CCTTAGTTGGGGATTAAGGA+TGG 0.516631 4.4:+41748289 MS.gene021147:CDS
ATTCATTGTGCCTTGGGAGA+AGG 0.517082 4.4:-41745845 None:intergenic
AGAATGGGGACTTCCTTGTA+GGG 0.517590 4.4:-41745885 None:intergenic
TTTGATGTGCACCTTAGTTG+GGG 0.517799 4.4:+41748278 MS.gene021147:CDS
ATGGCTTGGATTGTTGAGGA+TGG 0.518174 4.4:+41748308 MS.gene021147:CDS
TAGACCATATTTCACAGGGC+AGG 0.518387 4.4:+41747077 MS.gene021147:CDS
AAAATTGCATCCTCCTCCTA+AGG 0.524928 4.4:+41748346 MS.gene021147:CDS
GACAGATACATACAAATTGA+AGG 0.527305 4.4:+41746834 MS.gene021147:intron
CTAGCTAGACCATATTTCAC+AGG 0.529517 4.4:+41747072 MS.gene021147:CDS
TACAAATTGAAGGTGAAGCC+AGG 0.530121 4.4:+41746844 MS.gene021147:CDS
AGAACATTTGTGGTTTGACC+TGG 0.536892 4.4:-41747006 None:intergenic
CTAACCAAAGTATTGTTTGG+AGG 0.538509 4.4:-41747522 None:intergenic
TTGCACAAGCTCTGCAGACA+GGG 0.538737 4.4:+41746143 MS.gene021147:CDS
ACCGTGAATGGGAAGTTCCC+GGG 0.543276 4.4:+41745266 MS.gene021147:CDS
GAGTCCAATACCATCATACT+TGG 0.543746 4.4:+41746982 MS.gene021147:CDS
ATGCTAGTATTGACCCTGCA+AGG 0.544490 4.4:+41747691 MS.gene021147:CDS
CCTCGAATTGTTGTACGAGA+AGG 0.546915 4.4:+41745290 MS.gene021147:CDS
ACTGTTGCTGTGCCTTCTGG+TGG 0.553231 4.4:+41747744 MS.gene021147:CDS
CCTTTGGAGCAATTGTAAAG+AGG 0.556173 4.4:-41746214 None:intergenic
TTGGGGAGATCCTTAGGAGG+AGG 0.561208 4.4:-41748356 None:intergenic
TCTGCAGATAAGGTTGGCAA+AGG 0.561585 4.4:+41745211 MS.gene021147:intron
TGTCACTGTTCCATTATCAA+AGG 0.562747 4.4:+41744940 MS.gene021147:CDS
CGTACAACAATTCGAGGACC+CGG 0.566305 4.4:-41745284 None:intergenic
CCATCCTTAATCCCCAACTA+AGG 0.569779 4.4:-41748289 None:intergenic
TGAGACAACTTCGTAGTGGT+TGG 0.574177 4.4:+41745676 MS.gene021147:CDS
TTAAATCCATGAAGATGCAA+AGG 0.575251 4.4:-41747633 None:intergenic
TTCTAAAATTCCAGCTACTG+TGG 0.577452 4.4:-41747115 None:intergenic
AGGAAACCCATTAATGGTGT+AGG 0.579472 4.4:-41746188 None:intergenic
AAATTAGTATCATTGATGGC+TGG 0.579956 4.4:-41747195 None:intergenic
CAAACAATATCAGCCTGCAT+TGG 0.581284 4.4:+41745348 MS.gene021147:CDS
AATCACCTGGATTGTCAGCA+AGG 0.581837 4.4:-41747785 None:intergenic
TTGACCTGGTCCAAGTATGA+TGG 0.586103 4.4:-41746992 None:intergenic
GACCTCTCATCCTTCTCCCA+AGG 0.596454 4.4:+41745835 MS.gene021147:CDS
TCCAGGGGAGGTTAAGAGGA+AGG 0.601150 4.4:-41744993 None:intergenic
ACAACTTCGTAGTGGTTGGT+CGG 0.604046 4.4:+41745680 MS.gene021147:CDS
AAGTAGCCAAAACAAATCCA+GGG 0.606149 4.4:-41745009 None:intergenic
ATCTTGTTGATCCTGTTGAG+AGG 0.606779 4.4:+41747718 MS.gene021147:CDS
AGTTCCTCTTTGTCCAACAA+TGG 0.608427 4.4:-41745766 None:intergenic
AGACCATATTTCACAGGGCA+GGG 0.613998 4.4:+41747078 MS.gene021147:CDS
CAACAAGATTAAACCTTGCA+GGG 0.615814 4.4:-41747704 None:intergenic
TCGAATTGTTGTACGAGAAG+GGG 0.619585 4.4:+41745292 MS.gene021147:CDS
CATTTGGGGAGATCCTTAGG+AGG 0.640212 4.4:-41748359 None:intergenic
ATCGCAACCCAACCACCAGA+AGG 0.645168 4.4:-41747756 None:intergenic
TGCCTTGGGAGAAGGATGAG+AGG 0.645507 4.4:-41745837 None:intergenic
ATGATGGTATTGGACTCGAA+TGG 0.647830 4.4:-41746976 None:intergenic
CATATGTACCAAAGAGAATG+GGG 0.650276 4.4:-41745899 None:intergenic
TGGACTAGTTCCTAGTCCAA+CGG 0.652330 4.4:-41747310 None:intergenic
GACAAATATGCATAGGTGAG+TGG 0.653174 4.4:+41746095 MS.gene021147:intron
AGCCAAAACAAATCCAGGGG+AGG 0.654034 4.4:-41745005 None:intergenic
GTACAACAATTCGAGGACCC+GGG 0.658530 4.4:-41745283 None:intergenic
AAATATGCATAGGTGAGTGG+TGG 0.659012 4.4:+41746098 MS.gene021147:intron
TCTAGCTAGCATGTAGAATG+TGG 0.659413 4.4:-41747058 None:intergenic
TGCACAAGCTCTGCAGACAG+GGG 0.660853 4.4:+41746144 MS.gene021147:CDS
TAAGGTTGGCAAAGGTTACG+AGG 0.665174 4.4:+41745219 MS.gene021147:CDS
AAGATGCATCTGCTTCGACA+AGG 0.668532 4.4:-41746945 None:intergenic
GCACAAGCTCTGCAGACAGG+GGG 0.670081 4.4:+41746145 MS.gene021147:CDS
AACTAAGGTGCACATCAAAG+TGG 0.677837 4.4:-41748274 None:intergenic
TAGCTAGACCATATTTCACA+GGG 0.683532 4.4:+41747073 MS.gene021147:CDS
AATACCATCATACTTGGACC+AGG 0.697209 4.4:+41746988 MS.gene021147:CDS
TAGTAATGGAACAAAGACAG+TGG 0.719057 4.4:+41747542 MS.gene021147:CDS
AGTAGCCAAAACAAATCCAG+GGG 0.722267 4.4:-41745008 None:intergenic
AGGGGAGGTTAAGAGGAAGG+TGG 0.734018 4.4:-41744990 None:intergenic
GGTGTAGTAATCAACATTTG+GGG 0.734375 4.4:-41748373 None:intergenic
GGTGTGAGACAACTTCGTAG+TGG 0.741558 4.4:+41745672 MS.gene021147:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAATTTATTATACTC+TGG + chr4.4:41745950-41745969 MS.gene021147:intron 10.0%
!! TTTATTATAAATTTATACTG+AGG - chr4.4:41745935-41745954 None:intergenic 10.0%
!!! CATATAAATAACTTTTTTTT+AGG - chr4.4:41748148-41748167 None:intergenic 10.0%
!! AAAATAGTATATGATATGAA+AGG + chr4.4:41746048-41746067 MS.gene021147:intron 15.0%
!! TTAAGTGATAAATATTTGAA+AGG - chr4.4:41745104-41745123 None:intergenic 15.0%
!! TTTGAATTTGAACTATTATT+TGG - chr4.4:41747363-41747382 None:intergenic 15.0%
!!! ATTGACTTAATTATTTAATC+TGG - chr4.4:41748060-41748079 None:intergenic 15.0%
!!! TTTTTATTTGTTTGATCATT+TGG - chr4.4:41747150-41747169 None:intergenic 15.0%
!! AACAAAATTAGTATCATTGA+TGG - chr4.4:41747202-41747221 None:intergenic 20.0%
!! AATAATTCATTTCATGCATA+TGG - chr4.4:41745600-41745619 None:intergenic 20.0%
!! AATTATTTAATCTGGTCTAT+TGG - chr4.4:41748052-41748071 None:intergenic 20.0%
!! ACTACATATGTTATACAATT+TGG - chr4.4:41745146-41745165 None:intergenic 20.0%
!! TGTACTAATATTGACAATAA+TGG + chr4.4:41745619-41745638 MS.gene021147:intron 20.0%
!!! AAATTAGTTTGTAAAATGAG+AGG - chr4.4:41746718-41746737 None:intergenic 20.0%
!!! AACTAATAATGGTTTTACAA+CGG + chr4.4:41747455-41747474 MS.gene021147:CDS 20.0%
!!! AAGTTTTTGTCAATAGTTTT+AGG - chr4.4:41747282-41747301 None:intergenic 20.0%
!!! AGTTTTTAATAGAACATTTG+TGG - chr4.4:41747019-41747038 None:intergenic 20.0%
!!! TTTTATTATTTCTGCAGATA+AGG + chr4.4:41745201-41745220 MS.gene021147:intron 20.0%
!!! TTTTTGAAAGATGAATTGAT+TGG + chr4.4:41746794-41746813 MS.gene021147:intron 20.0%
! AACAATACTTTGGTTAGTAA+TGG + chr4.4:41747528-41747547 MS.gene021147:CDS 25.0%
! AGCTTATGAAATCAGTTTAT+AGG - chr4.4:41747921-41747940 None:intergenic 25.0%
! ATATTGTATGTTGTACATCA+TGG + chr4.4:41745559-41745578 MS.gene021147:intron 25.0%
! CATATGTTATACAATTTGGT+AGG - chr4.4:41745142-41745161 None:intergenic 25.0%
! GAATAATGACTAGAATTGAT+TGG + chr4.4:41746764-41746783 MS.gene021147:intron 25.0%
! TATCTTTGACAAATATGCAT+AGG + chr4.4:41746088-41746107 MS.gene021147:intron 25.0%
!! AACATTTTCTTTATCCTAAG+TGG - chr4.4:41746670-41746689 None:intergenic 25.0%
!! GGATTTAATTTCTTTGTTGT+TGG + chr4.4:41747648-41747667 MS.gene021147:CDS 25.0%
!!! AAAAACTTTTTCTTTACCGT+TGG + chr4.4:41747294-41747313 MS.gene021147:CDS 25.0%
!!! AAACTAAGTGTTGAGAAAAT+AGG + chr4.4:41745447-41745466 MS.gene021147:intron 25.0%
!!! ATCTATTTGCTATGAATGTA+TGG + chr4.4:41748223-41748242 MS.gene021147:intron 25.0%
!!! ATTGGTTTGTTATCATATCA+TGG + chr4.4:41746812-41746831 MS.gene021147:intron 25.0%
!!! CATTTCTTTGGAACTAATAA+TGG + chr4.4:41747444-41747463 MS.gene021147:CDS 25.0%
!!! CTGGAGTTTAAACTTTTTTA+AGG + chr4.4:41745989-41746008 MS.gene021147:intron 25.0%
!!! TGGTATTTTATATTTCTAGC+TGG + chr4.4:41745970-41745989 MS.gene021147:intron 25.0%
!!! TTACTAACCAAAGTATTGTT+TGG - chr4.4:41747528-41747547 None:intergenic 25.0%
AAAATAGTTCTTACCAATGC+AGG - chr4.4:41745364-41745383 None:intergenic 30.0%
GACAGATACATACAAATTGA+AGG + chr4.4:41746834-41746853 MS.gene021147:intron 30.0%
TAGTTCCAAAGAAATGTTGT+TGG - chr4.4:41747440-41747459 None:intergenic 30.0%
TATGAATGTATGGTTGAAAC+AGG + chr4.4:41748233-41748252 MS.gene021147:intron 30.0%
TCATATGTACCAAAGAGAAT+GGG - chr4.4:41745903-41745922 None:intergenic 30.0%
TGAGAAAATAGGATTTGAAC+TGG + chr4.4:41745458-41745477 MS.gene021147:intron 30.0%
TGCATGCATGATAAAAAAAC+AGG + chr4.4:41745646-41745665 MS.gene021147:intron 30.0%
TTAAATCCATGAAGATGCAA+AGG - chr4.4:41747636-41747655 None:intergenic 30.0%
TTCATATGTACCAAAGAGAA+TGG - chr4.4:41745904-41745923 None:intergenic 30.0%
! AAATTAGTATCATTGATGGC+TGG - chr4.4:41747198-41747217 None:intergenic 30.0%
! CGTAATAGATATGTTTTTCC+TGG - chr4.4:41746865-41746884 None:intergenic 30.0%
! TGTGAGTTTTGCAGTTAATT+AGG - chr4.4:41745426-41745445 None:intergenic 30.0%
!! AATTTCTTTGTTGTTGGTCA+AGG + chr4.4:41747654-41747673 MS.gene021147:CDS 30.0%
!! ATTATTTCTGCAGATAAGGT+TGG + chr4.4:41745205-41745224 MS.gene021147:intron 30.0%
!! CTTATTTTCGTAAGTTCTCT+AGG - chr4.4:41747946-41747965 None:intergenic 30.0%
!!! CTTGACAAGTTTGGTTTTTT+GGG - chr4.4:41747338-41747357 None:intergenic 30.0%
AACTCCTCCAAACAATACTT+TGG + chr4.4:41747518-41747537 MS.gene021147:CDS 35.0%
AACTCTGACCTGTTTGAATT+GGG - chr4.4:41745731-41745750 None:intergenic 35.0%
AAGTAGCCAAAACAAATCCA+GGG - chr4.4:41745012-41745031 None:intergenic 35.0%
AATGTGGCATTTGGGTAATT+TGG - chr4.4:41747045-41747064 None:intergenic 35.0%
ACAACGACATTTGATATGCT+TGG - chr4.4:41745487-41745506 None:intergenic 35.0%
ACCATATAACACTAGAGTTC+AGG + chr4.4:41747569-41747588 MS.gene021147:CDS 35.0%
ACCTGAACTCTAGTGTTATA+TGG - chr4.4:41747573-41747592 None:intergenic 35.0%
CAACAAGATTAAACCTTGCA+GGG - chr4.4:41747707-41747726 None:intergenic 35.0%
CAACGACATTTGATATGCTT+GGG - chr4.4:41745486-41745505 None:intergenic 35.0%
CATATGTACCAAAGAGAATG+GGG - chr4.4:41745902-41745921 None:intergenic 35.0%
CCAAAAAACCAAACTTGTCA+AGG + chr4.4:41747336-41747355 MS.gene021147:CDS 35.0%
CTTCTCCAACAACATTTCTT+TGG + chr4.4:41747432-41747451 MS.gene021147:CDS 35.0%
GAAAAAACCAAGTTCCACTT+AGG + chr4.4:41746653-41746672 MS.gene021147:intron 35.0%
GATAAGATTCATTGTGCCTT+GGG - chr4.4:41745854-41745873 None:intergenic 35.0%
GCAAAAAGAGCCTTTGATAA+TGG - chr4.4:41744953-41744972 None:intergenic 35.0%
GCATGATAAAAAAACAGGCA+TGG + chr4.4:41745651-41745670 MS.gene021147:intron 35.0%
GGAGTTCCTGTATAGTTAAA+CGG - chr4.4:41747504-41747523 None:intergenic 35.0%
GTATAGTTAAACGGCCTTAA+CGG - chr4.4:41747495-41747514 None:intergenic 35.0%
TAACTCTGACCTGTTTGAAT+TGG - chr4.4:41745732-41745751 None:intergenic 35.0%
TAGCTAGACCATATTTCACA+GGG + chr4.4:41747073-41747092 MS.gene021147:CDS 35.0%
TATTTGGTCCTTGACAAGTT+TGG - chr4.4:41747347-41747366 None:intergenic 35.0%
TCAACAAGATTAAACCTTGC+AGG - chr4.4:41747708-41747727 None:intergenic 35.0%
TCTTTATCCTAAGTGGAACT+TGG - chr4.4:41746663-41746682 None:intergenic 35.0%
TGTACATCATGGATCTAGTT+AGG + chr4.4:41745570-41745589 MS.gene021147:intron 35.0%
TGTAGGCATCAGAAACATTT+GGG - chr4.4:41746174-41746193 None:intergenic 35.0%
TGTCACTGTTCCATTATCAA+AGG + chr4.4:41744940-41744959 MS.gene021147:CDS 35.0%
TTAAGGCCGTTTAACTATAC+AGG + chr4.4:41747495-41747514 MS.gene021147:CDS 35.0%
! ATTGCAGGATACAAGCATTT+TGG + chr4.4:41747596-41747615 MS.gene021147:CDS 35.0%
! TAGAGTTCAGGTTATATTGC+AGG + chr4.4:41747581-41747600 MS.gene021147:CDS 35.0%
! TAGTAATGGAACAAAGACAG+TGG + chr4.4:41747542-41747561 MS.gene021147:CDS 35.0%
! TCCTTTTCAAAAACCCTACA+AGG + chr4.4:41745872-41745891 MS.gene021147:CDS 35.0%
! TGATTGACTATGGTTCTGTT+TGG + chr4.4:41747803-41747822 MS.gene021147:intron 35.0%
! TTGCAGGATACAAGCATTTT+GGG + chr4.4:41747597-41747616 MS.gene021147:CDS 35.0%
! TTTTTCTTTACCGTTGGACT+AGG + chr4.4:41747300-41747319 MS.gene021147:CDS 35.0%
!! CTAACCAAAGTATTGTTTGG+AGG - chr4.4:41747525-41747544 None:intergenic 35.0%
!! GGAGGATGCAATTTTTGATT+AGG - chr4.4:41748341-41748360 None:intergenic 35.0%
!! TCCTTGTAGGGTTTTTGAAA+AGG - chr4.4:41745876-41745895 None:intergenic 35.0%
!! TGGTTTTACAACGGATTTTC+CGG + chr4.4:41747464-41747483 MS.gene021147:CDS 35.0%
!! TTCTAAAATTCCAGCTACTG+TGG - chr4.4:41747118-41747137 None:intergenic 35.0%
!!! ATAGTTTTAGGCACATTTGC+AGG - chr4.4:41747270-41747289 None:intergenic 35.0%
!!! CCTTGACAAGTTTGGTTTTT+TGG - chr4.4:41747339-41747358 None:intergenic 35.0%
!!! GAAGCACAGGTTTTAGAATA+TGG - chr4.4:41747176-41747195 None:intergenic 35.0%
!!! TAGTTTTAGGCACATTTGCA+GGG - chr4.4:41747269-41747288 None:intergenic 35.0%
!!! TTTGTTGTTGGTCAAGGTTT+TGG + chr4.4:41747660-41747679 MS.gene021147:CDS 35.0%
AAAATTGCATCCTCCTCCTA+AGG + chr4.4:41748346-41748365 MS.gene021147:CDS 40.0%
AAAGCATGGTTACCGTGAAT+GGG + chr4.4:41745255-41745274 MS.gene021147:CDS 40.0%
AAATATGCATAGGTGAGTGG+TGG + chr4.4:41746098-41746117 MS.gene021147:intron 40.0%
AACTAAGGTGCACATCAAAG+TGG - chr4.4:41748277-41748296 None:intergenic 40.0%
AATACCATCATACTTGGACC+AGG + chr4.4:41746988-41747007 MS.gene021147:CDS 40.0%
ACAATCCAGGTGATTGACTA+TGG + chr4.4:41747793-41747812 MS.gene021147:intron 40.0%
ACGTGCAGCTTGTATATAAC+TGG + chr4.4:41746240-41746259 MS.gene021147:intron 40.0%
ACTCAATGCCCAATTCAAAC+AGG + chr4.4:41745720-41745739 MS.gene021147:CDS 40.0%
ACTTTGATGTGCACCTTAGT+TGG + chr4.4:41748276-41748295 MS.gene021147:CDS 40.0%
AGAACATTTGTGGTTTGACC+TGG - chr4.4:41747009-41747028 None:intergenic 40.0%
AGCATGTAGAATGTGGCATT+TGG - chr4.4:41747054-41747073 None:intergenic 40.0%
AGGAAACCCATTAATGGTGT+AGG - chr4.4:41746191-41746210 None:intergenic 40.0%
AGTAGCCAAAACAAATCCAG+GGG - chr4.4:41745011-41745030 None:intergenic 40.0%
AGTCATCCTTTGCATCTTCA+TGG + chr4.4:41747627-41747646 MS.gene021147:CDS 40.0%
AGTTCCTCTTTGTCCAACAA+TGG - chr4.4:41745769-41745788 None:intergenic 40.0%
ATCTTGTTGATCCTGTTGAG+AGG + chr4.4:41747718-41747737 MS.gene021147:CDS 40.0%
CAAACAATATCAGCCTGCAT+TGG + chr4.4:41745348-41745367 MS.gene021147:CDS 40.0%
CAATGCAGGCTGATATTGTT+TGG - chr4.4:41745350-41745369 None:intergenic 40.0%
CCTCTTTACAATTGCTCCAA+AGG + chr4.4:41746214-41746233 MS.gene021147:CDS 40.0%
CTAGCTAGACCATATTTCAC+AGG + chr4.4:41747072-41747091 MS.gene021147:CDS 40.0%
CTCGAATTGTTGTACGAGAA+GGG + chr4.4:41745291-41745310 MS.gene021147:CDS 40.0%
CTGATGCCTACACCATTAAT+GGG + chr4.4:41746182-41746201 MS.gene021147:CDS 40.0%
CTTTGATGTGCACCTTAGTT+GGG + chr4.4:41748277-41748296 MS.gene021147:CDS 40.0%
GAAGTAGCCAAAACAAATCC+AGG - chr4.4:41745013-41745032 None:intergenic 40.0%
GACAAATATGCATAGGTGAG+TGG + chr4.4:41746095-41746114 MS.gene021147:intron 40.0%
GAGTCCAATACCATCATACT+TGG + chr4.4:41746982-41747001 MS.gene021147:CDS 40.0%
GCATGTAGAATGTGGCATTT+GGG - chr4.4:41747053-41747072 None:intergenic 40.0%
GGATAAGATTCATTGTGCCT+TGG - chr4.4:41745855-41745874 None:intergenic 40.0%
GTATGGTTGAAACAGGTGTT+TGG + chr4.4:41748240-41748259 MS.gene021147:intron 40.0%
GTGTACAACTTCACCATTGT+TGG + chr4.4:41745753-41745772 MS.gene021147:CDS 40.0%
GTGTAGGCATCAGAAACATT+TGG - chr4.4:41746175-41746194 None:intergenic 40.0%
GTGTGTGAGAGTAAAACTCA+TGG - chr4.4:41745518-41745537 None:intergenic 40.0%
TACAAATTGAAGGTGAAGCC+AGG + chr4.4:41746844-41746863 MS.gene021147:CDS 40.0%
TCGAATTGTTGTACGAGAAG+GGG + chr4.4:41745292-41745311 MS.gene021147:CDS 40.0%
TCTAGCTAGCATGTAGAATG+TGG - chr4.4:41747061-41747080 None:intergenic 40.0%
TCTGATGCCTACACCATTAA+TGG + chr4.4:41746181-41746200 MS.gene021147:CDS 40.0%
TGGTCCAAGTATGATGGTAT+TGG - chr4.4:41746989-41747008 None:intergenic 40.0%
TGGTTAAGAGCTACTGTCTA+TGG + chr4.4:41745813-41745832 MS.gene021147:CDS 40.0%
TTCACCATTGTTGGACAAAG+AGG + chr4.4:41745762-41745781 MS.gene021147:CDS 40.0%
TTTGATGTGCACCTTAGTTG+GGG + chr4.4:41748278-41748297 MS.gene021147:CDS 40.0%
! AAAAGCATGGTTACCGTGAA+TGG + chr4.4:41745254-41745273 MS.gene021147:CDS 40.0%
! AGGGGACAGATTATTGGTTA+AGG + chr4.4:41745310-41745329 MS.gene021147:CDS 40.0%
! ATGATGGTATTGGACTCGAA+TGG - chr4.4:41746979-41746998 None:intergenic 40.0%
! ATTTTCCGGTTGTACCGTTA+AGG + chr4.4:41747478-41747497 MS.gene021147:CDS 40.0%
!! CCTTTGGAGCAATTGTAAAG+AGG - chr4.4:41746217-41746236 None:intergenic 40.0%
!! GGGCATTGAGTTATGTATGA+TGG - chr4.4:41745711-41745730 None:intergenic 40.0%
!! TACACCATTAATGGGTTTCC+TGG + chr4.4:41746190-41746209 MS.gene021147:CDS 40.0%
!! TGTTGGAGAAGTGCTATAGA+AGG - chr4.4:41747423-41747442 None:intergenic 40.0%
!!! CAAGTTTGGTTTTTTGGGCA+TGG - chr4.4:41747333-41747352 None:intergenic 40.0%
!!! GTTTTGGCTACTTCACAACA+TGG + chr4.4:41745019-41745038 MS.gene021147:CDS 40.0%
AAGATGCATCTGCTTCGACA+AGG - chr4.4:41746948-41746967 None:intergenic 45.0%
AAGGAAGTCCCCATTCTCTT+TGG + chr4.4:41745891-41745910 MS.gene021147:CDS 45.0%
AATACTGTTGCTGTGCCTTC+TGG + chr4.4:41747741-41747760 MS.gene021147:CDS 45.0%
AATCACCTGGATTGTCAGCA+AGG - chr4.4:41747788-41747807 None:intergenic 45.0%
ACAACTTCGTAGTGGTTGGT+CGG + chr4.4:41745680-41745699 MS.gene021147:CDS 45.0%
ACGAGAAGGGGACAGATTAT+TGG + chr4.4:41745304-41745323 MS.gene021147:CDS 45.0%
AGAATGGGGACTTCCTTGTA+GGG - chr4.4:41745888-41745907 None:intergenic 45.0%
AGACCATATTTCACAGGGCA+GGG + chr4.4:41747078-41747097 MS.gene021147:CDS 45.0%
AGGTCCAGGAAACCCATTAA+TGG - chr4.4:41746197-41746216 None:intergenic 45.0%
ATTCATTGTGCCTTGGGAGA+AGG - chr4.4:41745848-41745867 None:intergenic 45.0%
CAACATTTGGGGAGATCCTT+AGG - chr4.4:41748365-41748384 None:intergenic 45.0%
CAGAACCATAGTCAATCACC+TGG - chr4.4:41747801-41747820 None:intergenic 45.0%
CCATCCTTAATCCCCAACTA+AGG - chr4.4:41748292-41748311 None:intergenic 45.0%
CCTCGAATTGTTGTACGAGA+AGG + chr4.4:41745290-41745309 MS.gene021147:CDS 45.0%
CCTTAGTTGGGGATTAAGGA+TGG + chr4.4:41748289-41748308 MS.gene021147:CDS 45.0%
CCTTCTCGTACAACAATTCG+AGG - chr4.4:41745293-41745312 None:intergenic 45.0%
GACAAAGAGGAACTCTCTTC+TGG + chr4.4:41745775-41745794 MS.gene021147:CDS 45.0%
GAGCAATTGTAAAGAGGTCC+AGG - chr4.4:41746211-41746230 None:intergenic 45.0%
GCAACAGTATTCCTCTCAAC+AGG - chr4.4:41747732-41747751 None:intergenic 45.0%
GCTTGTGCAATTACAGCTTC+AGG - chr4.4:41746133-41746152 None:intergenic 45.0%
TAAGGTTGGCAAAGGTTACG+AGG + chr4.4:41745219-41745238 MS.gene021147:CDS 45.0%
TAGACCATATTTCACAGGGC+AGG + chr4.4:41747077-41747096 MS.gene021147:CDS 45.0%
TCTGCAGATAAGGTTGGCAA+AGG + chr4.4:41745211-41745230 MS.gene021147:intron 45.0%
TCTGGCATGCTCATTTCTCA+TGG + chr4.4:41745793-41745812 MS.gene021147:CDS 45.0%
TGAGACAACTTCGTAGTGGT+TGG + chr4.4:41745676-41745695 MS.gene021147:CDS 45.0%
TGCACCTTAGTTGGGGATTA+AGG + chr4.4:41748285-41748304 MS.gene021147:CDS 45.0%
TTCGACAATTCCACAGTAGC+TGG + chr4.4:41747105-41747124 MS.gene021147:CDS 45.0%
TTGACCTGGTCCAAGTATGA+TGG - chr4.4:41746995-41747014 None:intergenic 45.0%
! AAGGATGGCTTGGATTGTTG+AGG + chr4.4:41748304-41748323 MS.gene021147:CDS 45.0%
! ATGGCTTGGATTGTTGAGGA+TGG + chr4.4:41748308-41748327 MS.gene021147:CDS 45.0%
!! AACCTCCCCTGGATTTGTTT+TGG + chr4.4:41745003-41745022 MS.gene021147:CDS 45.0%
!! ATGCTAGTATTGACCCTGCA+AGG + chr4.4:41747691-41747710 MS.gene021147:CDS 45.0%
!! CGGTAACCATGCTTTTTGTG+TGG - chr4.4:41745250-41745269 None:intergenic 45.0%
!! GGCACATTTGCAGGGTATTT+TGG - chr4.4:41747261-41747280 None:intergenic 45.0%
!! TGGACTAGTTCCTAGTCCAA+CGG - chr4.4:41747313-41747332 None:intergenic 45.0%
!! TAAAATATTATTAATTAAAG+TGG - chr4.4:41746618-41746637 None:intergenic 5.0%
AACGGCCTTAACGGTACAAC+CGG - chr4.4:41747486-41747505 None:intergenic 50.0%
ACAAGCTGCACGTGTACCTT+TGG - chr4.4:41746233-41746252 None:intergenic 50.0%
AGCCAAAACAAATCCAGGGG+AGG - chr4.4:41745008-41745027 None:intergenic 50.0%
ATTGCACAAGCTCTGCAGAC+AGG + chr4.4:41746142-41746161 MS.gene021147:CDS 50.0%
CAAATCCAGGGGAGGTTAAG+AGG - chr4.4:41745000-41745019 None:intergenic 50.0%
CATTTGGGGAGATCCTTAGG+AGG - chr4.4:41748362-41748381 None:intergenic 50.0%
CGATACCTTGCTGACAATCC+AGG + chr4.4:41747780-41747799 MS.gene021147:CDS 50.0%
CGTACAACAATTCGAGGACC+CGG - chr4.4:41745287-41745306 None:intergenic 50.0%
GAGAATGGGGACTTCCTTGT+AGG - chr4.4:41745889-41745908 None:intergenic 50.0%
GCTGTGCCACACAAAAAGCA+TGG + chr4.4:41745241-41745260 MS.gene021147:CDS 50.0%
GGTGTGAGACAACTTCGTAG+TGG + chr4.4:41745672-41745691 MS.gene021147:CDS 50.0%
GTACAACAATTCGAGGACCC+GGG - chr4.4:41745286-41745305 None:intergenic 50.0%
GTACCCTGCCCTGTGAAATA+TGG - chr4.4:41747084-41747103 None:intergenic 50.0%
GTTGGGGATTAAGGATGGCT+TGG + chr4.4:41748294-41748313 MS.gene021147:CDS 50.0%
TACCGTGAATGGGAAGTTCC+CGG + chr4.4:41745265-41745284 MS.gene021147:CDS 50.0%
TTGCACAAGCTCTGCAGACA+GGG + chr4.4:41746143-41746162 MS.gene021147:CDS 50.0%
! TCATTGATGGCTGGAAGCAC+AGG - chr4.4:41747189-41747208 None:intergenic 50.0%
ACCCGGGAACTTCCCATTCA+CGG - chr4.4:41745270-41745289 None:intergenic 55.0%
ACCGTGAATGGGAAGTTCCC+GGG + chr4.4:41745266-41745285 MS.gene021147:CDS 55.0%
ACCTTCCTCTTAACCTCCCC+TGG + chr4.4:41744992-41745011 MS.gene021147:CDS 55.0%
ACTGTTGCTGTGCCTTCTGG+TGG + chr4.4:41747744-41747763 MS.gene021147:CDS 55.0%
AGGGGAGGTTAAGAGGAAGG+TGG - chr4.4:41744993-41745012 None:intergenic 55.0%
ATCGCAACCCAACCACCAGA+AGG - chr4.4:41747759-41747778 None:intergenic 55.0%
GACCTCTCATCCTTCTCCCA+AGG + chr4.4:41745835-41745854 MS.gene021147:CDS 55.0%
TCCAGGGGAGGTTAAGAGGA+AGG - chr4.4:41744996-41745015 None:intergenic 55.0%
TGCACAAGCTCTGCAGACAG+GGG + chr4.4:41746144-41746163 MS.gene021147:CDS 55.0%
TGCCTTGGGAGAAGGATGAG+AGG - chr4.4:41745840-41745859 None:intergenic 55.0%
TGCTGTGCCTTCTGGTGGTT+GGG + chr4.4:41747749-41747768 MS.gene021147:CDS 55.0%
TTGCTGTGCCTTCTGGTGGT+TGG + chr4.4:41747748-41747767 MS.gene021147:CDS 55.0%
TTGGGGAGATCCTTAGGAGG+AGG - chr4.4:41748359-41748378 None:intergenic 55.0%
! CTTCGTAGTGGTTGGTCGGA+TGG + chr4.4:41745684-41745703 MS.gene021147:CDS 55.0%
GCACAAGCTCTGCAGACAGG+GGG + chr4.4:41746145-41746164 MS.gene021147:CDS 60.0%
CAAGCTCTGCAGACAGGGGG+TGG + chr4.4:41746148-41746167 MS.gene021147:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 41744923 41748385 41744923 ID=MS.gene021147
chr4.4 mRNA 41744923 41748385 41744923 ID=MS.gene021147.t1;Parent=MS.gene021147
chr4.4 exon 41744923 41745069 41744923 ID=MS.gene021147.t1.exon1;Parent=MS.gene021147.t1
chr4.4 CDS 41744923 41745069 41744923 ID=cds.MS.gene021147.t1;Parent=MS.gene021147.t1
chr4.4 exon 41745218 41745369 41745218 ID=MS.gene021147.t1.exon2;Parent=MS.gene021147.t1
chr4.4 CDS 41745218 41745369 41745218 ID=cds.MS.gene021147.t1;Parent=MS.gene021147.t1
chr4.4 exon 41745668 41745912 41745668 ID=MS.gene021147.t1.exon3;Parent=MS.gene021147.t1
chr4.4 CDS 41745668 41745912 41745668 ID=cds.MS.gene021147.t1;Parent=MS.gene021147.t1
chr4.4 exon 41746110 41746235 41746110 ID=MS.gene021147.t1.exon4;Parent=MS.gene021147.t1
chr4.4 CDS 41746110 41746235 41746110 ID=cds.MS.gene021147.t1;Parent=MS.gene021147.t1
chr4.4 exon 41746839 41747801 41746839 ID=MS.gene021147.t1.exon5;Parent=MS.gene021147.t1
chr4.4 CDS 41746839 41747801 41746839 ID=cds.MS.gene021147.t1;Parent=MS.gene021147.t1
chr4.4 exon 41748255 41748385 41748255 ID=MS.gene021147.t1.exon6;Parent=MS.gene021147.t1
chr4.4 CDS 41748255 41748385 41748255 ID=cds.MS.gene021147.t1;Parent=MS.gene021147.t1
Gene Sequence

>MS.gene021147

ATGCATATCAAAATGATTGTCACTGTTCCATTATCAAAGGCTCTTTTTGCAGCTTTTCTCTTTGTCACCACCTTCCTCTTAACCTCCCCTGGATTTGTTTTGGCTACTTCACAACATGGAAGTGTTACAAGACACTACAAATTTGATGTATATACACTTTATATTATATAAATTAATTCCTTTCAAATATTTATCACTTAATTAATTAACTTAAAACCTACCAAATTGTATAACATATGTAGTGTAAGAAAAAAAAAAGTTTTATAGTATATCATTGTTTTTATTATTTCTGCAGATAAGGTTGGCAAAGGTTACGAGGCTGTGCCACACAAAAAGCATGGTTACCGTGAATGGGAAGTTCCCGGGTCCTCGAATTGTTGTACGAGAAGGGGACAGATTATTGGTTAAGGTTGTTAATCATGTTCCAAACAATATCAGCCTGCATTGGTAAGAACTATTTTTTTTAATTATTTGTAATTTTTCACATTTTTTTCAAAGAGCCTAATTAACTGCAAAACTCACAAAAACTAAGTGTTGAGAAAATAGGATTTGAACTGGCGCCCAAGCATATCAAATGTCGTTGTAGTTTTCACCATGAGTTTTACTCTCACACACACGCAATTTTTTTTTACATTTATATTGTATGTTGTACATCATGGATCTAGTTAGGATATCCATATGCATGAAATGAATTATTGTACTAATATTGACAATAATGGTGAATGCATGCATGATAAAAAAACAGGCATGGTGTGAGACAACTTCGTAGTGGTTGGTCGGATGGTCCATCATACATAACTCAATGCCCAATTCAAACAGGTCAGAGTTATGTGTACAACTTCACCATTGTTGGACAAAGAGGAACTCTCTTCTGGCATGCTCATTTCTCATGGTTAAGAGCTACTGTCTATGGACCTCTCATCCTTCTCCCAAGGCACAATGAATCTTATCCTTTTCAAAAACCCTACAAGGAAGTCCCCATTCTCTTTGGTACATATGAACAATCATTCCTCAGTATAAATTTATAATAAATAATTTATTATACTCTGGTATTTTATATTTCTAGCTGGAGTTTAAACTTTTTTAAGGAAAGATATAATAAAGATATTTTTTTTATTTTACAAAAAAATAGTATATGATATGAAAGGTTTTGTAATCTTATACATATCTTTGACAAATATGCATAGGTGAGTGGTGGAATGCAGATCCTGAAGCTGTAATTGCACAAGCTCTGCAGACAGGGGGTGGCCCAAATGTTTCTGATGCCTACACCATTAATGGGTTTCCTGGACCTCTTTACAATTGCTCCAAAGGTACACGTGCAGCTTGTATATAACTGGCATTTCTAGTTATTTGTTTTTAATTAAGAACTAAATAAATTTTGTACATTTAATGTAATTTTTTTTACACTAATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTAACATAACAAAGTGATATGATACTAAAATGTGATTTAATTGAATTTATGTTTAAAAAAGTTCTACATAGTTAAAGAGTTTTTTTTTCTTTCTCAATTCATTAAATAGAAGTAAATTGATTATATGTTGTTTATTTAGCTTAATTTCGTTTATAAATAACACAATATCTTACAAGTTGCTTTGCTTAACACCACTTTAATTAATAATATTTTATTTTATTTTATTAGAGAAAAAACCAAGTTCCACTTAGGATAAAGAAAATGTTTGAAATGAGTTTATAAATAGTGACACCTCTCATTTTACAAACTAATTTGTAAAAATCGAGTTAGATAAATTGACGAATAATGACTAGAATTGATTGGTTTTTTTTTTTTGAAAGATGAATTGATTGGTTTGTTATCATATCATGGACAGATACATACAAATTGAAGGTGAAGCCAGGAAAAACATATCTATTACGTTTGATCAACGCTGCACTCAACGACGAACTCTTTTTCAGCATTGCTAATCACACATTAATCCTTGTCGAAGCAGATGCATCTTACATTAAACCATTCGAGTCCAATACCATCATACTTGGACCAGGTCAAACCACAAATGTTCTATTAAAAACTAAACCAAATTACCCAAATGCCACATTCTACATGCTAGCTAGACCATATTTCACAGGGCAGGGTACTTTCGACAATTCCACAGTAGCTGGAATTTTAGAATACACTAAACCAAATGATCAAACAAATAAAAATCTCCATATTCTAAAACCTGTGCTTCCAGCCATCAATGATACTAATTTTGTTGCTAATTTTAGTAACAAATTTCTTAGCTTAAATAGTCCAAAATACCCTGCAAATGTGCCTAAAACTATTGACAAAAACTTTTTCTTTACCGTTGGACTAGGAACTAGTCCATGCCCAAAAAACCAAACTTGTCAAGGACCAAATAATAGTTCAAATTCAAAATTTGCAGCTTCAATGAACAATGTATCATTTGCTTTACCTTCTATAGCACTTCTCCAACAACATTTCTTTGGAACTAATAATGGTTTTACAACGGATTTTCCGGTTGTACCGTTAAGGCCGTTTAACTATACAGGAACTCCTCCAAACAATACTTTGGTTAGTAATGGAACAAAGACAGTGGTGATACCATATAACACTAGAGTTCAGGTTATATTGCAGGATACAAGCATTTTGGGAGCTGAGAGTCATCCTTTGCATCTTCATGGATTTAATTTCTTTGTTGTTGGTCAAGGTTTTGGAAATTTTAATGCTAGTATTGACCCTGCAAGGTTTAATCTTGTTGATCCTGTTGAGAGGAATACTGTTGCTGTGCCTTCTGGTGGTTGGGTTGCGATACGATACCTTGCTGACAATCCAGGTGATTGACTATGGTTCTGTTTGGATAATCATCTTAATTAAGCACTTATCATATAAGCGTTTATGCATAGCTATTTCTATAACAAATGACAAAAAAGTCAAAGTATTTTCATATAACCTATAAACTGATTTCATAAGCTGTCCTAGAGAACTTACGAAAATAAGTTAAAAACAATTTATAGACATTGTCGTAAGTTGTTTTCAATTTATTTTATAAGCTCTTCTAAACAGTCTTACAAGTGTCTATGCCAATAGACCAGATTAAATAATTAAGTCAATCGAAATATTACATATCATATAGTTTTTCTTATCTTGTATCTCAAGTTATTTATATGATACTTTCACCTAAAAAAAAGTTATTTATATGATACTTTGATCACTTTAATTATAAAGCATATAATGAATTAATTAGACTTTTTTTGATCTATTTGCTATGAATGTATGGTTGAAACAGGTGTTTGGCTGATGCATTGCCACTTTGATGTGCACCTTAGTTGGGGATTAAGGATGGCTTGGATTGTTGAGGATGGAAAGTTACCTAATCAAAAATTGCATCCTCCTCCTAAGGATCTCCCCAAATGTTGA
Protein sequence

>MS.gene021147.t1

MHIKMIVTVPLSKALFAAFLFVTTFLLTSPGFVLATSQHGSVTRHYKFDIRLAKVTRLCHTKSMVTVNGKFPGPRIVVREGDRLLVKVVNHVPNNISLHWHGVRQLRSGWSDGPSYITQCPIQTGQSYVYNFTIVGQRGTLFWHAHFSWLRATVYGPLILLPRHNESYPFQKPYKEVPILFGEWWNADPEAVIAQALQTGGGPNVSDAYTINGFPGPLYNCSKDTYKLKVKPGKTYLLRLINAALNDELFFSIANHTLILVEADASYIKPFESNTIILGPGQTTNVLLKTKPNYPNATFYMLARPYFTGQGTFDNSTVAGILEYTKPNDQTNKNLHILKPVLPAINDTNFVANFSNKFLSLNSPKYPANVPKTIDKNFFFTVGLGTSPCPKNQTCQGPNNSSNSKFAASMNNVSFALPSIALLQQHFFGTNNGFTTDFPVVPLRPFNYTGTPPNNTLVSNGTKTVVIPYNTRVQVILQDTSILGAESHPLHLHGFNFFVVGQGFGNFNASIDPARFNLVDPVERNTVAVPSGGWVAIRYLADNPGVWLMHCHFDVHLSWGLRMAWIVEDGKLPNQKLHPPPKDLPKC