Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26401.t1 | 1ZGJ_A | 85.8 | 239 | 3 | 1 | 1 | 208 | 116 | 354 | 2.60E-108 | 401.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26401.t1 | Q29U70 | 85.8 | 239 | 3 | 1 | 1 | 208 | 126 | 364 | 6.3e-111 | 401.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26401.t1 | A0A072UNK8 | 85.8 | 239 | 3 | 1 | 1 | 208 | 126 | 364 | 1.9e-108 | 401.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050883 | MS.gene26401 | 0.841888 | 3.62E-58 | -1.69E-46 |
| MS.gene051127 | MS.gene26401 | 0.804992 | 1.66E-49 | -1.69E-46 |
| MS.gene051341 | MS.gene26401 | 0.830142 | 3.47E-55 | -1.69E-46 |
| MS.gene055551 | MS.gene26401 | 0.805021 | 1.63E-49 | -1.69E-46 |
| MS.gene055961 | MS.gene26401 | 0.803201 | 3.90E-49 | -1.69E-46 |
| MS.gene056310 | MS.gene26401 | 0.813062 | 3.09E-51 | -1.69E-46 |
| MS.gene056311 | MS.gene26401 | 0.820546 | 6.48E-53 | -1.69E-46 |
| MS.gene056530 | MS.gene26401 | 0.80132 | 9.52E-49 | -1.69E-46 |
| MS.gene058109 | MS.gene26401 | 0.828755 | 7.54E-55 | -1.69E-46 |
| MS.gene058111 | MS.gene26401 | 0.822177 | 2.72E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene26401 | 0.817862 | 2.65E-52 | -1.69E-46 |
| MS.gene058173 | MS.gene26401 | 0.817117 | 3.89E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene26401 | 0.817305 | 3.53E-52 | -1.69E-46 |
| MS.gene058493 | MS.gene26401 | 0.806685 | 7.30E-50 | -1.69E-46 |
| MS.gene058628 | MS.gene26401 | 0.823419 | 1.40E-53 | -1.69E-46 |
| MS.gene058629 | MS.gene26401 | 0.820017 | 8.57E-53 | -1.69E-46 |
| MS.gene058630 | MS.gene26401 | 0.827116 | 1.87E-54 | -1.69E-46 |
| MS.gene059045 | MS.gene26401 | 0.80376 | 2.99E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGTTCTTCAATATCTTTA+AGG | 0.195840 | 4.1:+22119872 | None:intergenic |
| GTGCAACAGGGGAGAGCTTT+TGG | 0.222303 | 4.1:-22120550 | MS.gene26401:CDS |
| CGAACACGCGTTTGTTCTCT+TGG | 0.271846 | 4.1:+22120438 | None:intergenic |
| CAAACGCGTGTTCGAGGGTT+TGG | 0.283225 | 4.1:-22120429 | MS.gene26401:CDS |
| TACAGTGGGTTCTGCATGAT+TGG | 0.326455 | 4.1:-22119908 | MS.gene26401:intron |
| ACACTGTTTGAGTGTGCAAC+AGG | 0.344866 | 4.1:-22120563 | MS.gene26401:CDS |
| GTGATGCTTACAATGTTTCT+TGG | 0.364082 | 4.1:-22119735 | MS.gene26401:CDS |
| TGGATTAACTGAACTGCAAT+TGG | 0.377228 | 4.1:-22119769 | MS.gene26401:CDS |
| AAAACAGTGCATTTAAGGTT+AGG | 0.380135 | 4.1:+22120344 | None:intergenic |
| GTTGATGTTGGAGGTGGTAC+TGG | 0.401447 | 4.1:-22120398 | MS.gene26401:CDS |
| TTGGAGTCTCTTGTTGATGT+TGG | 0.404723 | 4.1:-22120410 | MS.gene26401:CDS |
| CTCCACATGTCTAAAGAACT+TGG | 0.410016 | 4.1:+22120623 | None:intergenic |
| AAGAAAGAACAAAGCAAGAA+TGG | 0.426132 | 4.1:-22119710 | MS.gene26401:CDS |
| ACTGCAATTGGACTATGATT+TGG | 0.427028 | 4.1:-22119757 | MS.gene26401:CDS |
| GAGAGACTTGAAGCCAGAAA+TGG | 0.427559 | 4.1:+22119641 | None:intergenic |
| AGAGACTTGAAGCCAGAAAT+GGG | 0.439445 | 4.1:+22119642 | None:intergenic |
| AGAGAACAAACGCGTGTTCG+AGG | 0.444395 | 4.1:-22120435 | MS.gene26401:CDS |
| TGTGGAGTTCCTCAGAGAAA+TGG | 0.491399 | 4.1:-22120607 | MS.gene26401:CDS |
| GAGTCTCTTGTTGATGTTGG+AGG | 0.496314 | 4.1:-22120407 | MS.gene26401:CDS |
| ATCCAAGTTCTTTAGACATG+TGG | 0.496638 | 4.1:-22120625 | MS.gene26401:CDS |
| GAGTATGTTTCAAGATGCTA+TGG | 0.498973 | 4.1:-22120492 | MS.gene26401:CDS |
| AGAAAGAACAAAGCAAGAAT+GGG | 0.507206 | 4.1:-22119709 | MS.gene26401:CDS |
| GAAATGGTTCAATGAAGATA+AGG | 0.516608 | 4.1:-22120591 | MS.gene26401:CDS |
| GAGAACAAACGCGTGTTCGA+GGG | 0.542946 | 4.1:-22120434 | MS.gene26401:CDS |
| ATTTCACACAAAGGAAAGGA+TGG | 0.547784 | 4.1:-22119840 | MS.gene26401:CDS |
| TCTCTTGTTGATGTTGGAGG+TGG | 0.554073 | 4.1:-22120404 | MS.gene26401:CDS |
| AGCAATTTCACACAAAGGAA+AGG | 0.577136 | 4.1:-22119844 | MS.gene26401:CDS |
| CACTGTTTGAGTGTGCAACA+GGG | 0.603315 | 4.1:-22120562 | MS.gene26401:CDS |
| GATGTTGGAGGTGGTACTGG+TGG | 0.610342 | 4.1:-22120395 | MS.gene26401:CDS |
| TTCATTGAACCATTTCTCTG+AGG | 0.619737 | 4.1:+22120598 | None:intergenic |
| AAAGAAGCAATTTCACACAA+AGG | 0.626707 | 4.1:-22119849 | MS.gene26401:CDS |
| GAGACTTGAAGCCAGAAATG+GGG | 0.636393 | 4.1:+22119643 | None:intergenic |
| AGACTTGAAGCCAGAAATGG+GGG | 0.646680 | 4.1:+22119644 | None:intergenic |
| ACTGTTTGAGTGTGCAACAG+GGG | 0.649035 | 4.1:-22120561 | MS.gene26401:CDS |
| GATGAAACAAGTGACGATCG+TGG | 0.671140 | 4.1:-22119789 | MS.gene26401:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATATAACTTTTACTAATA+AGG | - | chr4.1:22120242-22120261 | MS.gene26401:intron | 10.0% |
| !!! | AATTGTTACTATTTCAATTT+TGG | - | chr4.1:22120057-22120076 | MS.gene26401:intron | 15.0% |
| !!! | TGACATTTAATTTAGTTATT+TGG | + | chr4.1:22120317-22120336 | None:intergenic | 15.0% |
| !!! | TTTTACTAATAAGGATAAAA+TGG | - | chr4.1:22120251-22120270 | MS.gene26401:intron | 15.0% |
| !! | AAGTTATATAACAAGATTGA+AGG | + | chr4.1:22120233-22120252 | None:intergenic | 20.0% |
| !! | AATGAAAATTTGAACTTTGT+TGG | - | chr4.1:22119970-22119989 | MS.gene26401:intron | 20.0% |
| !!! | ACAAATTAAGATGCATATTT+TGG | - | chr4.1:22120286-22120305 | MS.gene26401:intron | 20.0% |
| ! | TGTTAGACATTAAAATGTGT+AGG | - | chr4.1:22120199-22120218 | MS.gene26401:intron | 25.0% |
| !! | CAATTGTCTTTTATTACAGT+GGG | - | chr4.1:22120335-22120354 | MS.gene26401:CDS | 25.0% |
| !! | TCAATTGTCTTTTATTACAG+TGG | - | chr4.1:22120334-22120353 | MS.gene26401:CDS | 25.0% |
| !! | TCGTTGACTAAATTTAATTC+TGG | + | chr4.1:22120139-22120158 | None:intergenic | 25.0% |
| AAAGAAGCAATTTCACACAA+AGG | - | chr4.1:22120408-22120427 | MS.gene26401:CDS | 30.0% | |
| AAGAAAGAACAAAGCAAGAA+TGG | - | chr4.1:22120547-22120566 | MS.gene26401:CDS | 30.0% | |
| AGAAAGAACAAAGCAAGAAT+GGG | - | chr4.1:22120548-22120567 | MS.gene26401:CDS | 30.0% | |
| GAAAATTTGAACTTTGTTGG+TGG | - | chr4.1:22119973-22119992 | MS.gene26401:intron | 30.0% | |
| GAAATGGTTCAATGAAGATA+AGG | - | chr4.1:22119666-22119685 | MS.gene26401:CDS | 30.0% | |
| GATCAAAAACAGTGCATTTA+AGG | + | chr4.1:22119921-22119940 | None:intergenic | 30.0% | |
| GCTGTTCTTCAATATCTTTA+AGG | + | chr4.1:22120388-22120407 | None:intergenic | 30.0% | |
| ! | AAAACAGTGCATTTAAGGTT+AGG | + | chr4.1:22119916-22119935 | None:intergenic | 30.0% |
| ACTGCAATTGGACTATGATT+TGG | - | chr4.1:22120500-22120519 | MS.gene26401:CDS | 35.0% | |
| AGCAATTTCACACAAAGGAA+AGG | - | chr4.1:22120413-22120432 | MS.gene26401:CDS | 35.0% | |
| ATCCAAGTTCTTTAGACATG+TGG | - | chr4.1:22119632-22119651 | MS.gene26401:CDS | 35.0% | |
| ATTTCACACAAAGGAAAGGA+TGG | - | chr4.1:22120417-22120436 | MS.gene26401:CDS | 35.0% | |
| GAAAAACTCATATACGATGC+AGG | - | chr4.1:22120570-22120589 | MS.gene26401:CDS | 35.0% | |
| GAGTATGTTTCAAGATGCTA+TGG | - | chr4.1:22119765-22119784 | MS.gene26401:CDS | 35.0% | |
| GTCATTGTCAATATTGTGTC+CGG | + | chr4.1:22120109-22120128 | None:intergenic | 35.0% | |
| GTGATGCTTACAATGTTTCT+TGG | - | chr4.1:22120522-22120541 | MS.gene26401:CDS | 35.0% | |
| TACAAAATTACCCCCATTTC+TGG | - | chr4.1:22120603-22120622 | MS.gene26401:CDS | 35.0% | |
| TGGATTAACTGAACTGCAAT+TGG | - | chr4.1:22120488-22120507 | MS.gene26401:CDS | 35.0% | |
| TTCATTGAACCATTTCTCTG+AGG | + | chr4.1:22119662-22119681 | None:intergenic | 35.0% | |
| !! | CAATTTTGGTCTATGAAGCA+CGG | - | chr4.1:22120071-22120090 | MS.gene26401:intron | 35.0% |
| !!! | TGCTGATGCTGTTTTACTTA+AGG | - | chr4.1:22120020-22120039 | MS.gene26401:intron | 35.0% |
| AGAGACTTGAAGCCAGAAAT+GGG | + | chr4.1:22120618-22120637 | None:intergenic | 40.0% | |
| AGTAAAACAGCATCAGCAGA+AGG | + | chr4.1:22120018-22120037 | None:intergenic | 40.0% | |
| CTCCACATGTCTAAAGAACT+TGG | + | chr4.1:22119637-22119656 | None:intergenic | 40.0% | |
| GTAAAACAGCATCAGCAGAA+GGG | + | chr4.1:22120017-22120036 | None:intergenic | 40.0% | |
| GTTAAGTTACCAACAACCTG+TGG | + | chr4.1:22119946-22119965 | None:intergenic | 40.0% | |
| TTGGAGTCTCTTGTTGATGT+TGG | - | chr4.1:22119847-22119866 | MS.gene26401:CDS | 40.0% | |
| TTTGATCAACCACAGGTTGT+TGG | - | chr4.1:22119934-22119953 | MS.gene26401:intron | 40.0% | |
| !! | CAGGTTGTTGGTAACTTAAC+TGG | - | chr4.1:22119946-22119965 | MS.gene26401:intron | 40.0% |
| !!! | CACTGTTTTTGATCAACCAC+AGG | - | chr4.1:22119927-22119946 | MS.gene26401:intron | 40.0% |
| ACACTGTTTGAGTGTGCAAC+AGG | - | chr4.1:22119694-22119713 | MS.gene26401:CDS | 45.0% | |
| ACTGTTTGAGTGTGCAACAG+GGG | - | chr4.1:22119696-22119715 | MS.gene26401:CDS | 45.0% | |
| AGACTTGAAGCCAGAAATGG+GGG | + | chr4.1:22120616-22120635 | None:intergenic | 45.0% | |
| CACTGTTTGAGTGTGCAACA+GGG | - | chr4.1:22119695-22119714 | MS.gene26401:CDS | 45.0% | |
| GAGACTTGAAGCCAGAAATG+GGG | + | chr4.1:22120617-22120636 | None:intergenic | 45.0% | |
| GAGAGACTTGAAGCCAGAAA+TGG | + | chr4.1:22120619-22120638 | None:intergenic | 45.0% | |
| GAGTCTCTTGTTGATGTTGG+AGG | - | chr4.1:22119850-22119869 | MS.gene26401:CDS | 45.0% | |
| GATGAAACAAGTGACGATCG+TGG | - | chr4.1:22120468-22120487 | MS.gene26401:CDS | 45.0% | |
| TCTCTTGTTGATGTTGGAGG+TGG | - | chr4.1:22119853-22119872 | MS.gene26401:CDS | 45.0% | |
| TGTGGAGTTCCTCAGAGAAA+TGG | - | chr4.1:22119650-22119669 | MS.gene26401:CDS | 45.0% | |
| ! | TACAGTGGGTTCTGCATGAT+TGG | - | chr4.1:22120349-22120368 | MS.gene26401:CDS | 45.0% |
| AGAGAACAAACGCGTGTTCG+AGG | - | chr4.1:22119822-22119841 | MS.gene26401:CDS | 50.0% | |
| CGAACACGCGTTTGTTCTCT+TGG | + | chr4.1:22119822-22119841 | None:intergenic | 50.0% | |
| GAGAACAAACGCGTGTTCGA+GGG | - | chr4.1:22119823-22119842 | MS.gene26401:CDS | 50.0% | |
| GTTGATGTTGGAGGTGGTAC+TGG | - | chr4.1:22119859-22119878 | MS.gene26401:CDS | 50.0% | |
| ! | TGCAACAGGGGAGAGCTTTT+GGG | - | chr4.1:22119708-22119727 | MS.gene26401:CDS | 50.0% |
| CAAACGCGTGTTCGAGGGTT+TGG | - | chr4.1:22119828-22119847 | MS.gene26401:CDS | 55.0% | |
| ! | GATGTTGGAGGTGGTACTGG+TGG | - | chr4.1:22119862-22119881 | MS.gene26401:CDS | 55.0% |
| ! | GTGCAACAGGGGAGAGCTTT+TGG | - | chr4.1:22119707-22119726 | MS.gene26401:CDS | 55.0% |
| AGCACGGACACTGCACACAC+CGG | - | chr4.1:22120087-22120106 | MS.gene26401:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 22119623 | 22120656 | 22119623 | ID=MS.gene26401 |
| chr4.1 | mRNA | 22119623 | 22120656 | 22119623 | ID=MS.gene26401.t1;Parent=MS.gene26401 |
| chr4.1 | exon | 22120331 | 22120656 | 22120331 | ID=MS.gene26401.t1.exon1;Parent=MS.gene26401.t1 |
| chr4.1 | CDS | 22120331 | 22120656 | 22120331 | ID=cds.MS.gene26401.t1;Parent=MS.gene26401.t1 |
| chr4.1 | exon | 22119623 | 22119925 | 22119623 | ID=MS.gene26401.t1.exon2;Parent=MS.gene26401.t1 |
| chr4.1 | CDS | 22119623 | 22119925 | 22119623 | ID=cds.MS.gene26401.t1;Parent=MS.gene26401.t1 |
| Gene Sequence |
| Protein sequence |
