Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26552.t1 | AES90241.1 | 100 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 2.10E-20 | 107.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26552.t1 | G7JMI7 | 100.0 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 1.5e-20 | 107.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene26552 | 0.813016 | 3.17E-51 | -1.69E-46 |
| MS.gene049424 | MS.gene26552 | 0.801253 | 9.83E-49 | -1.69E-46 |
| MS.gene049653 | MS.gene26552 | 0.809072 | 2.27E-50 | -1.69E-46 |
| MS.gene049951 | MS.gene26552 | 0.800426 | 1.45E-48 | -1.69E-46 |
| MS.gene050559 | MS.gene26552 | 0.809434 | 1.90E-50 | -1.69E-46 |
| MS.gene050945 | MS.gene26552 | 0.807058 | 6.08E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene26552 | 0.826052 | 3.35E-54 | -1.69E-46 |
| MS.gene052500 | MS.gene26552 | 0.828477 | 8.80E-55 | -1.69E-46 |
| MS.gene052502 | MS.gene26552 | 0.816568 | 5.17E-52 | -1.69E-46 |
| MS.gene052826 | MS.gene26552 | 0.82289 | 1.86E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene26552 | 0.842029 | 3.32E-58 | -1.69E-46 |
| MS.gene053931 | MS.gene26552 | 0.808369 | 3.20E-50 | -1.69E-46 |
| MS.gene054957 | MS.gene26552 | 0.807499 | 4.91E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene26552 | 0.809484 | 1.85E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene26552 | 0.814243 | 1.70E-51 | -1.69E-46 |
| MS.gene057392 | MS.gene26552 | 0.837974 | 3.79E-57 | -1.69E-46 |
| MS.gene05985 | MS.gene26552 | -0.802453 | 5.57E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene26552 | 0.80324 | 3.83E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26552.t1 | MTR_4g087080 | 100.000 | 55 | 0 | 0 | 1 | 55 | 1 | 55 | 5.15e-35 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACGGTTGATTGGTGAAAA+AGG | 0.267840 | 4.1:+22982832 | None:intergenic |
| AATACTCCTCTCCCTTTCTA+TGG | 0.290250 | 4.1:-22982808 | MS.gene26552:CDS |
| AATAGAATTGTGCATATCTA+TGG | 0.361157 | 4.1:-22982926 | MS.gene26552:CDS |
| AACTGCCATAACAAATTCTA+TGG | 0.401714 | 4.1:+22982894 | None:intergenic |
| GGAGTATTTGCACGGTTGAT+TGG | 0.424542 | 4.1:+22982823 | None:intergenic |
| AATGGCCATAGAATTTGTTA+TGG | 0.435872 | 4.1:-22982899 | MS.gene26552:CDS |
| GTGCATATCTATGGTTAGAA+TGG | 0.466421 | 4.1:-22982917 | MS.gene26552:CDS |
| CAACGGAGGTAACCATAGAA+AGG | 0.524840 | 4.1:+22982796 | None:intergenic |
| AACGGAGGTAACCATAGAAA+GGG | 0.543015 | 4.1:+22982797 | None:intergenic |
| AGGTAACCATAGAAAGGGAG+AGG | 0.582749 | 4.1:+22982802 | None:intergenic |
| AAGGGAGAGGAGTATTTGCA+CGG | 0.631175 | 4.1:+22982815 | None:intergenic |
| AATTCAATTCAACTCAACGG+AGG | 0.680829 | 4.1:+22982782 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATAGAATTGTGCATATCTA+TGG | - | chr4.1:22982809-22982828 | MS.gene26552:CDS | 25.0% |
| AACTGCCATAACAAATTCTA+TGG | + | chr4.1:22982844-22982863 | None:intergenic | 30.0% | |
| !! | AATGGCCATAGAATTTGTTA+TGG | - | chr4.1:22982836-22982855 | MS.gene26552:CDS | 30.0% |
| GTGCATATCTATGGTTAGAA+TGG | - | chr4.1:22982818-22982837 | MS.gene26552:CDS | 35.0% | |
| AACGGAGGTAACCATAGAAA+GGG | + | chr4.1:22982941-22982960 | None:intergenic | 40.0% | |
| AATACTCCTCTCCCTTTCTA+TGG | - | chr4.1:22982927-22982946 | MS.gene26552:CDS | 40.0% | |
| AGGTAACCATAGAAAGGGAG+AGG | + | chr4.1:22982936-22982955 | None:intergenic | 45.0% | |
| CAACGGAGGTAACCATAGAA+AGG | + | chr4.1:22982942-22982961 | None:intergenic | 45.0% | |
| GCACGGTTGATTGGTGAAAA+AGG | + | chr4.1:22982906-22982925 | None:intergenic | 45.0% | |
| ! | GGAGTATTTGCACGGTTGAT+TGG | + | chr4.1:22982915-22982934 | None:intergenic | 45.0% |
| !! | AAGGGAGAGGAGTATTTGCA+CGG | + | chr4.1:22982923-22982942 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 22982795 | 22982962 | 22982795 | ID=MS.gene26552 |
| chr4.1 | mRNA | 22982795 | 22982962 | 22982795 | ID=MS.gene26552.t1;Parent=MS.gene26552 |
| chr4.1 | exon | 22982795 | 22982962 | 22982795 | ID=MS.gene26552.t1.exon1;Parent=MS.gene26552.t1 |
| chr4.1 | CDS | 22982795 | 22982962 | 22982795 | ID=cds.MS.gene26552.t1;Parent=MS.gene26552.t1 |
| Gene Sequence |
| Protein sequence |
