AlfalfaGEDB | MS.gene049951

M. sativa cultivar XinJiangDaYe / MS.gene049951

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049951.t1	XP_003615340.1	94.2	86	5	0	3	88	6	91	9.00E-34	152.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049951.t1	G7JYH8	94.2	86	5	0	3	88	6	91	6.5e-34	152.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049653	MS.gene049951	0.809656	1.70E-50	-1.69E-46
MS.gene049951	MS.gene057392	0.807006	6.24E-50	-1.69E-46
MS.gene049951	MS.gene057999	0.803152	4.00E-49	-1.69E-46
MS.gene049951	MS.gene063288	0.853927	1.73E-61	-1.69E-46
MS.gene049951	MS.gene066362	0.806928	6.48E-50	-1.69E-46
MS.gene049951	MS.gene067635	0.822434	2.37E-53	-1.69E-46
MS.gene049951	MS.gene068164	0.802065	6.69E-49	-1.69E-46
MS.gene049951	MS.gene068166	0.812241	4.68E-51	-1.69E-46
MS.gene049951	MS.gene068565	0.800863	1.18E-48	-1.69E-46
MS.gene049951	MS.gene069828	0.822221	2.66E-53	-1.69E-46
MS.gene049951	MS.gene071975	0.806348	8.59E-50	-1.69E-46
MS.gene049951	MS.gene073674	0.815619	8.43E-52	-1.69E-46
MS.gene049951	MS.gene26552	0.800426	1.45E-48	-1.69E-46
MS.gene049951	MS.gene36311	0.801067	1.07E-48	-1.69E-46
MS.gene049951	MS.gene39287	0.800194	1.62E-48	-1.69E-46
MS.gene049951	MS.gene45652	0.807425	5.09E-50	-1.69E-46
MS.gene049951	MS.gene47818	0.815474	9.08E-52	-1.69E-46
MS.gene049951	MS.gene53935	0.800165	1.64E-48	-1.69E-46
MS.gene049951	MS.gene56285	0.801	1.11E-48	-1.69E-46
MS.gene049951	MS.gene56656	0.800829	1.20E-48	-1.69E-46
MS.gene049951	MS.gene59505	0.818148	2.28E-52	-1.69E-46
MS.gene049951	MS.gene59616	0.800237	1.58E-48	-1.69E-46
MS.gene049951	MS.gene63673	0.824157	9.40E-54	-1.69E-46
MS.gene049951	MS.gene64806	0.818536	1.86E-52	-1.69E-46
MS.gene049951	MS.gene85109	0.80018	1.63E-48	-1.69E-46
MS.gene049951	MS.gene85111	0.800923	1.15E-48	-1.69E-46
MS.gene049951	MS.gene87292	0.800758	1.24E-48	-1.69E-46
MS.gene049951	MS.gene88148	0.812388	4.35E-51	-1.69E-46
MS.gene049951	MS.gene91942	0.821227	4.52E-53	-1.69E-46
MS.gene049951	MS.gene93983	0.825443	4.68E-54	-1.69E-46
MS.gene049951	MS.gene97348	0.800703	1.27E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049951.t1	MTR_5g066760	96.250	80	3	0	9	88	12	91	7.19e-53	160

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049951.t1	AT2G38465	59.420	69	28	0	16	84	13	81	7.34e-23	84.0

Find 6 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATCACAACAAAACTTCTTT+TGG	0.285814	5.3:+52546988	MS.gene049951:CDS
CATTAATTTAATTAACTATG+AGG	0.415967	5.3:-52547327	None:intergenic
CTGCTGAACATATCAAGAAT+AGG	0.429170	5.3:+52547295	MS.gene049951:CDS
AAAAGATTCTTGTATAGAAA+CGG	0.487491	5.3:-52547043	None:intergenic
GGCTTCAAAGAAACAAGTTG+AGG	0.622095	5.3:+52547263	MS.gene049951:CDS
AGCAAGTGAAGCTGAACTTG+AGG	0.634101	5.3:+52547242	MS.gene049951:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAGATTCTTGTATAGAAA+CGG	-	chr5.3:52547046-52547065	None:intergenic	20.0%
!!	AAATAAGTAGTAGCTAAAAA+TGG	-	chr5.3:52547128-52547147	None:intergenic	20.0%
!	GAATTGGTGTGAAAAAAAAT+AGG	+	chr5.3:52547188-52547207	MS.gene049951:intron	25.0%
!	TAGTAGCTAAAAATGGAAAA+GGG	-	chr5.3:52547121-52547140	None:intergenic	25.0%
!	TATAACTGCAAAAAATGAGA+AGG	+	chr5.3:52547218-52547237	MS.gene049951:CDS	25.0%
!!	AATCACAACAAAACTTCTTT+TGG	+	chr5.3:52546988-52547007	MS.gene049951:CDS	25.0%
!!!	AAAGAAGTTTTGTTGTGATT+TGG	-	chr5.3:52546988-52547007	None:intergenic	25.0%
	ATCAAACAAGGCAATTTCAA+AGG	-	chr5.3:52547164-52547183	None:intergenic	30.0%
	GTAGTAGCTAAAAATGGAAA+AGG	-	chr5.3:52547122-52547141	None:intergenic	30.0%
!!	AAATGGAAAAGGGTTGTTTT+TGG	-	chr5.3:52547111-52547130	None:intergenic	30.0%
!!!	TATGCAAAAGCCTTTTTTGT+CGG	+	chr5.3:52547069-52547088	MS.gene049951:CDS	30.0%
	AATTACCTGACCGACAAAAA+AGG	-	chr5.3:52547082-52547101	None:intergenic	35.0%
	ACCAATTCCTCAATCAAACA+AGG	-	chr5.3:52547176-52547195	None:intergenic	35.0%
	CTGCTGAACATATCAAGAAT+AGG	+	chr5.3:52547295-52547314	MS.gene049951:CDS	35.0%
!!	GAAATTGCCTTGTTTGATTG+AGG	+	chr5.3:52547166-52547185	MS.gene049951:intron	35.0%
!!	GTTTTGTTGTGATTTGGCAA+AGG	-	chr5.3:52546982-52547001	None:intergenic	35.0%
	GGCTTCAAAGAAACAAGTTG+AGG	+	chr5.3:52547263-52547282	MS.gene049951:CDS	40.0%
!!	GCCTTGTTTGATTGAGGAAT+TGG	+	chr5.3:52547172-52547191	MS.gene049951:intron	40.0%
!!!	AAAAGCCTTTTTTGTCGGTC+AGG	+	chr5.3:52547074-52547093	MS.gene049951:CDS	40.0%
	AGCAAGTGAAGCTGAACTTG+AGG	+	chr5.3:52547242-52547261	MS.gene049951:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	52546958	52547338	52546958	ID=MS.gene049951
chr5.3	mRNA	52546958	52547338	52546958	ID=MS.gene049951.t1;Parent=MS.gene049951
chr5.3	exon	52546958	52547095	52546958	ID=MS.gene049951.t1.exon1;Parent=MS.gene049951.t1
chr5.3	CDS	52546958	52547095	52546958	ID=cds.MS.gene049951.t1;Parent=MS.gene049951.t1
chr5.3	exon	52547210	52547338	52547210	ID=MS.gene049951.t1.exon2;Parent=MS.gene049951.t1
chr5.3	CDS	52547210	52547338	52547210	ID=cds.MS.gene049951.t1;Parent=MS.gene049951.t1

Gene Sequence

>MS.gene049951

ATGGGTTCTTCATCGACAACACCTTTGCCAAATCACAACAAAACTTCTTTTGGTACTCGTTTGAAGATCAGTTGCTTATCTTTTGCCGTTTCTATACAAGAATCTTTTAGATATGCAAAAGCCTTTTTTGTCGGTCAGGTAATTCAATATCCAAAAACAACCCTTTTCCATTTTTAGCTACTACTTATTTCAATATTATTGTTCCTTTGAAATTGCCTTGTTTGATTGAGGAATTGGTGTGAAAAAAAATAGGCAAAGACTATAACTGCAAAAAATGAGAAGGAAGCAAGTGAAGCTGAACTTGAGGCTTCAAAGAAACAAGTTGAGGCTGCTGATGCTGCTGAACATATCAAGAATAGGCTCAATGATCCTCATAGTTAA

Protein sequence

>MS.gene049951.t1

MGSSSTTPLPNHNKTSFGTRLKISCLSFAVSIQESFRYAKAFFVGQAKTITAKNEKEASEAELEASKKQVEAADAAEHIKNRLNDPHS