AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26688

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26688.t1 MTR_4g124910 95.257 253 12 0 1 253 1 253 1.37e-174 481
MS.gene26688.t1 MTR_2g043230 76.378 254 59 1 1 253 1 254 6.61e-142 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26688.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382
MS.gene26688.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382
MS.gene26688.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382
MS.gene26688.t1 AT5G20720 75.294 255 59 2 1 253 1 253 1.20e-135 382

Find 64 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTCATTCCCTGCATATT+TGG 0.259970 8.4:-9737127 None:intergenic
AACTGTATTCCCGGGTGAAA+TGG 0.321202 8.4:-9737097 None:intergenic
CCTCGTAACAATGCTAGAAT+TGG 0.335567 8.4:+9735217 MS.gene26688:CDS
TTCCCGGGTGAAATGGATAA+TGG 0.363835 8.4:-9737090 None:intergenic
TTTAAGATCTTTGACCTCTT+CGG 0.402758 8.4:-9736354 None:intergenic
AGGAACCTTTCTTCCTTTGA+AGG 0.413360 8.4:+9735169 MS.gene26688:CDS
GGAGTACAAAACTGTATTCC+CGG 0.420138 8.4:-9737106 None:intergenic
TATCAAGACTCTGTCATTCA+AGG 0.421253 8.4:-9736378 None:intergenic
CCTTCGTCATCCACAGGCCC+TGG 0.444066 8.4:-9737057 None:intergenic
GGCTTTGATTGAGCAGTTGA+TGG 0.449739 8.4:-9736067 None:intergenic
CTGTTGCCTTCGTCATCCAC+AGG 0.452383 8.4:-9737063 None:intergenic
CAGGTGATAGCTGTTGGTCC+AGG 0.460455 8.4:+9737039 MS.gene26688:intron
GAATGACAGAGTCTTGATAA+AGG 0.473936 8.4:+9736382 MS.gene26688:CDS
TTGGAGACGGGAAGACAGTT+GGG 0.483437 8.4:+9736113 MS.gene26688:CDS
GCAATCCTTCAAAGGAAGAA+AGG 0.496887 8.4:-9735174 None:intergenic
GCCAATGGATGGCTTATCCT+TGG 0.505865 8.4:-9736616 None:intergenic
AATGACTTCAAAGGCAAAGA+TGG 0.508584 8.4:+9737141 MS.gene26688:CDS
GAGGTAGTTGCTGTTGGAGA+CGG 0.521962 8.4:+9736100 MS.gene26688:CDS
GTTGGAGACGGGAAGACAGT+TGG 0.523055 8.4:+9736112 MS.gene26688:CDS
TGCTGGTGGTTTGTTGCTCA+CGG 0.524264 8.4:+9736587 MS.gene26688:CDS
ATCAAGACTCTGTCATTCAA+GGG 0.529761 8.4:-9736377 None:intergenic
CTGCTCAATCAAAGCCTCAA+GGG 0.533192 8.4:+9736074 MS.gene26688:CDS
AGGTAGTTGCTGTTGGAGAC+GGG 0.539082 8.4:+9736101 MS.gene26688:CDS
GCCACTAGGTGATAGAGTAC+TGG 0.539283 8.4:+9735997 MS.gene26688:CDS
AAGCTTACCGTGCCAATGGA+TGG 0.542126 8.4:-9736627 None:intergenic
AGAAAAGCTTACCGTGCCAA+TGG 0.548046 8.4:-9736631 None:intergenic
GGAACTGAAGTGGAGTTCAA+TGG 0.549387 8.4:+9736281 MS.gene26688:CDS
AGCTGTTGGTCCAGGGCCTG+TGG 0.552111 8.4:+9737047 MS.gene26688:CDS
GGAAGTTCGCAATCCTTCAA+AGG 0.561777 8.4:-9735182 None:intergenic
GCGGCTTTAACAACAATAGA+CGG 0.565089 8.4:-9735262 None:intergenic
TTCAAAACATCTTATACTGA+AGG 0.568271 8.4:+9736304 MS.gene26688:CDS
CATACAACTGTCAAGCCACT+AGG 0.568378 8.4:+9735983 MS.gene26688:CDS
ACCAAGGATAAGCCATCCAT+TGG 0.568643 8.4:+9736615 MS.gene26688:CDS
TATGCAGGGAATGACTTCAA+AGG 0.569312 8.4:+9737132 MS.gene26688:CDS
TTTGAGAGCCTCAGATGTCA+TGG 0.571249 8.4:+9737179 MS.gene26688:CDS
GAGTACAAAACTGTATTCCC+GGG 0.572521 8.4:-9737105 None:intergenic
CTGAAGGATGATGACATCGT+TGG 0.572824 8.4:+9736320 MS.gene26688:CDS
TGCTCAATCAAAGCCTCAAG+GGG 0.578517 8.4:+9736075 MS.gene26688:CDS
AGGTGATAGCTGTTGGTCCA+GGG 0.579593 8.4:+9737040 MS.gene26688:intron
AAAGTATGCAGGAACTGAAG+TGG 0.581171 8.4:+9736271 MS.gene26688:CDS
ACTGCTCAATCAAAGCCTCA+AGG 0.582194 8.4:+9736073 MS.gene26688:CDS
GTTGTGTACTCAAAGTATGC+AGG 0.590902 8.4:+9736260 MS.gene26688:CDS
AAACCATTATCCATTTCACC+CGG 0.592297 8.4:+9737087 MS.gene26688:CDS
CGGCTTTAACAACAATAGAC+GGG 0.595648 8.4:-9735261 None:intergenic
GGATGCAGAAGAGAAAACTC+AGG 0.597980 8.4:+9736030 MS.gene26688:CDS
ACCAGTACTCTATCACCTAG+TGG 0.604909 8.4:-9735998 None:intergenic
CAAAGTGGACATTAGTGTGA+AGG 0.607471 8.4:+9736141 MS.gene26688:CDS
GAATTGGTATTCTTACACAA+AGG 0.614844 8.4:+9735233 MS.gene26688:CDS
CCAGGGCCTGTGGATGACGA+AGG 0.620963 8.4:+9737057 MS.gene26688:CDS
AAAATAGCCAAAATTAAGAG+AGG 0.622578 8.4:-9737206 None:intergenic
GTTGCTCACGGAAGCAACCA+AGG 0.626344 8.4:+9736599 MS.gene26688:CDS
GAGGATAGCCATGACATCTG+AGG 0.634831 8.4:-9737187 None:intergenic
CCAATTCTAGCATTGTTACG+AGG 0.641555 8.4:-9735217 None:intergenic
AACCATTATCCATTTCACCC+GGG 0.641591 8.4:+9737088 MS.gene26688:CDS
GTTACGAGGAAAATGAATTG+CGG 0.642882 8.4:-9735203 None:intergenic
GGATAAGCCATCCATTGGCA+CGG 0.647992 8.4:+9736620 MS.gene26688:CDS
CTGCTTCATCGATTTCTACG+AGG 0.656045 8.4:+9735149 MS.gene26688:CDS
TGGTATTCTTGAAACCGAAG+AGG 0.662324 8.4:+9736340 MS.gene26688:CDS
GCTGCAGGTTGCAGAAGCTG+AGG 0.680718 8.4:+9736557 MS.gene26688:intron
GATGCAGAAGAGAAAACTCA+GGG 0.684794 8.4:+9736031 MS.gene26688:CDS
ATGTAACAGGTGATAGCTGT+TGG 0.691284 8.4:+9737033 MS.gene26688:intron
GACAGTTGGGAAGAACAAAG+TGG 0.692016 8.4:+9736126 MS.gene26688:CDS
GCAGAAGAGAAAACTCAGGG+TGG 0.728959 8.4:+9736034 MS.gene26688:CDS
GCTCAATCAAAGCCTCAAGG+GGG 0.730531 8.4:+9736076 MS.gene26688:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAAATAATACCT+TGG - chr8.4:9735302-9735321 None:intergenic 10.0%
!! AAAAATAAAAATAATACCTT+GGG - chr8.4:9735301-9735320 None:intergenic 10.0%
!!! AAGGTATTATTTTTATTTTT+TGG + chr8.4:9735301-9735320 MS.gene26688:intron 10.0%
!!! AGGTATTATTTTTATTTTTT+GGG + chr8.4:9735302-9735321 MS.gene26688:intron 10.0%
!!! TTTTCAAAAAATTAAAACAA+TGG + chr8.4:9736770-9736789 MS.gene26688:intron 10.0%
!! AAAATAAAAATAATACCTTG+GGG - chr8.4:9735300-9735319 None:intergenic 15.0%
!! CGAAAAATATTCATAAATAT+TGG - chr8.4:9735778-9735797 None:intergenic 15.0%
!! GTTAAGACAAATAATTAAAT+GGG - chr8.4:9736464-9736483 None:intergenic 15.0%
!! AAATAAAAATAATACCTTGG+GGG - chr8.4:9735299-9735318 None:intergenic 20.0%
!! AAGTATACTGTTTCAAAAAA+AGG - chr8.4:9736662-9736681 None:intergenic 20.0%
!! ACAAGATGAAATTACTAATA+AGG - chr8.4:9735961-9735980 None:intergenic 20.0%
!! CAAAAAATTAAAACAATGGA+TGG + chr8.4:9736774-9736793 MS.gene26688:intron 20.0%
!! GAAAAATATTCAAAAATGGT+GGG - chr8.4:9735752-9735771 None:intergenic 20.0%
!! GGTTAAGACAAATAATTAAA+TGG - chr8.4:9736465-9736484 None:intergenic 20.0%
!! GTCTGAAAAATATTCAAAAA+TGG - chr8.4:9735756-9735775 None:intergenic 20.0%
!! TGAAAAATATTCAAAAATGG+TGG - chr8.4:9735753-9735772 None:intergenic 20.0%
!! TTCAGACCAATAAAAAAAAA+GGG + chr8.4:9735678-9735697 MS.gene26688:intron 20.0%
!! TTTCAGACCAATAAAAAAAA+AGG + chr8.4:9735677-9735696 MS.gene26688:intron 20.0%
!!! TACATTTTCTCTTATACTAA+GGG - chr8.4:9735570-9735589 None:intergenic 20.0%
!!! TATTTTTATTTTTTGGGTCA+TGG + chr8.4:9735308-9735327 MS.gene26688:intron 20.0%
! TAACACCTTAATGCAAAATA+TGG - chr8.4:9736486-9736505 None:intergenic 25.0%
! TGTAATGTATTTCTCTTTAC+AGG + chr8.4:9736226-9736245 MS.gene26688:intron 25.0%
! TTCAAAACATCTTATACTGA+AGG + chr8.4:9736304-9736323 MS.gene26688:CDS 25.0%
!! ACATTTTCTCTTATACTAAG+GGG - chr8.4:9735569-9735588 None:intergenic 25.0%
!! CTTAACCATATTTTGCATTA+AGG + chr8.4:9736478-9736497 MS.gene26688:intron 25.0%
!! GTACATTTTCTCTTATACTA+AGG - chr8.4:9735571-9735590 None:intergenic 25.0%
!! TTTATTTATTGCTTTAGCTC+AGG + chr8.4:9736916-9736935 MS.gene26688:intron 25.0%
AAACACTAGAAATAATCCCA+TGG - chr8.4:9735903-9735922 None:intergenic 30.0%
AACACTAGAAATAATCCCAT+GGG - chr8.4:9735902-9735921 None:intergenic 30.0%
AATAAAAAAAAAGGGCAACC+CGG + chr8.4:9735686-9735705 MS.gene26688:intron 30.0%
AATTAAAACAATGGATGGTC+TGG + chr8.4:9736779-9736798 MS.gene26688:intron 30.0%
AGAATTGCAAGAATGTGATT+TGG + chr8.4:9736743-9736762 MS.gene26688:intron 30.0%
CCAAAATAAAAGAAAGACCA+AGG - chr8.4:9736201-9736220 None:intergenic 30.0%
GAATGGTTATTTACATACCT+AGG - chr8.4:9737001-9737020 None:intergenic 30.0%
GAATTGCAAGAATGTGATTT+GGG + chr8.4:9736744-9736763 MS.gene26688:intron 30.0%
GAATTGGTATTCTTACACAA+AGG + chr8.4:9735233-9735252 MS.gene26688:CDS 30.0%
GCAATGTTATATCGTATGAA+GGG + chr8.4:9736711-9736730 MS.gene26688:intron 30.0%
GTTGTGTTTATAAGCTATCT+TGG + chr8.4:9735421-9735440 MS.gene26688:intron 30.0%
TTGAAAGAGCTTATAGCTTA+TGG - chr8.4:9735488-9735507 None:intergenic 30.0%
! AATAACCATTCTACTCCTTT+TGG + chr8.4:9737010-9737029 MS.gene26688:intron 30.0%
! CATTAAGGTGTTACGTTTAA+AGG + chr8.4:9736493-9736512 MS.gene26688:intron 30.0%
! TGGATTTTCTTCATTATCTG+CGG + chr8.4:9735328-9735347 MS.gene26688:intron 30.0%
! TTTAAGATCTTTGACCTCTT+CGG - chr8.4:9736357-9736376 None:intergenic 30.0%
! TTTTGTACTCCAAATATGCA+GGG + chr8.4:9737118-9737137 MS.gene26688:CDS 30.0%
!! CCTTGGTCTTTCTTTTATTT+TGG + chr8.4:9736198-9736217 MS.gene26688:intron 30.0%
!! TAGAGTACTGGTAAAAATCA+AGG + chr8.4:9736009-9736028 MS.gene26688:CDS 30.0%
!!! TTGTTTTGATCGAGTAGTTT+TGG + chr8.4:9736974-9736993 MS.gene26688:intron 30.0%
AAACCATTATCCATTTCACC+CGG + chr8.4:9737087-9737106 MS.gene26688:CDS 35.0%
AAAGAAAGACCAAGGCTTAA+AGG - chr8.4:9736193-9736212 None:intergenic 35.0%
AACACATTCTACCGTCTTAA+GGG - chr8.4:9736871-9736890 None:intergenic 35.0%
AATGACTTCAAAGGCAAAGA+TGG + chr8.4:9737141-9737160 MS.gene26688:CDS 35.0%
ACCTGCAGCATCATAATTAA+AGG - chr8.4:9736546-9736565 None:intergenic 35.0%
ACTAGCATTATCCCTTAAGA+CGG + chr8.4:9736857-9736876 MS.gene26688:intron 35.0%
ATCAAGACTCTGTCATTCAA+GGG - chr8.4:9736380-9736399 None:intergenic 35.0%
ATCTCATAATGCACTTGAGT+GGG - chr8.4:9735514-9735533 None:intergenic 35.0%
ATGTATTTCTCTTTACAGGC+AGG + chr8.4:9736230-9736249 MS.gene26688:intron 35.0%
GAATGACAGAGTCTTGATAA+AGG + chr8.4:9736382-9736401 MS.gene26688:CDS 35.0%
GGCAATGTTATATCGTATGA+AGG + chr8.4:9736710-9736729 MS.gene26688:intron 35.0%
GTTACGAGGAAAATGAATTG+CGG - chr8.4:9735206-9735225 None:intergenic 35.0%
TAAAGGATTCCATAAGCTAG+TGG - chr8.4:9736176-9736195 None:intergenic 35.0%
TACATCCAAAAGGAGTAGAA+TGG - chr8.4:9737018-9737037 None:intergenic 35.0%
TATCAAGACTCTGTCATTCA+AGG - chr8.4:9736381-9736400 None:intergenic 35.0%
TATCACCTGTTACATCCAAA+AGG - chr8.4:9737028-9737047 None:intergenic 35.0%
TATCTCATAATGCACTTGAG+TGG - chr8.4:9735515-9735534 None:intergenic 35.0%
TGTTATATCGTATGAAGGGA+AGG + chr8.4:9736715-9736734 MS.gene26688:intron 35.0%
TTATGGAATCCTTTAAGCCT+TGG + chr8.4:9736181-9736200 MS.gene26688:intron 35.0%
! AACACCACTCTTCTAAAAGA+GGG + chr8.4:9736689-9736708 MS.gene26688:intron 35.0%
! GTTTTGTACTCCAAATATGC+AGG + chr8.4:9737117-9737136 MS.gene26688:CDS 35.0%
! TAACACCACTCTTCTAAAAG+AGG + chr8.4:9736688-9736707 MS.gene26688:intron 35.0%
!!! GGGTTGCCCTTTTTTTTTAT+TGG - chr8.4:9735687-9735706 None:intergenic 35.0%
AAAGTATGCAGGAACTGAAG+TGG + chr8.4:9736271-9736290 MS.gene26688:CDS 40.0%
AACCATTATCCATTTCACCC+GGG + chr8.4:9737088-9737107 MS.gene26688:CDS 40.0%
ACAGAGTCTTGATAAAGGTG+TGG + chr8.4:9736387-9736406 MS.gene26688:intron 40.0%
AGAAATAATCCCATGGGACA+GGG - chr8.4:9735896-9735915 None:intergenic 40.0%
AGGAACCTTTCTTCCTTTGA+AGG + chr8.4:9735169-9735188 MS.gene26688:CDS 40.0%
ATGTAACAGGTGATAGCTGT+TGG + chr8.4:9737033-9737052 MS.gene26688:intron 40.0%
CAAAGTGGACATTAGTGTGA+AGG + chr8.4:9736141-9736160 MS.gene26688:CDS 40.0%
CAACACATTCTACCGTCTTA+AGG - chr8.4:9736872-9736891 None:intergenic 40.0%
CCAATTCTAGCATTGTTACG+AGG - chr8.4:9735220-9735239 None:intergenic 40.0%
CCTCGTAACAATGCTAGAAT+TGG + chr8.4:9735217-9735236 MS.gene26688:CDS 40.0%
GAAGTCATTCCCTGCATATT+TGG - chr8.4:9737130-9737149 None:intergenic 40.0%
GAGTACAAAACTGTATTCCC+GGG - chr8.4:9737108-9737127 None:intergenic 40.0%
GATGCAGAAGAGAAAACTCA+GGG + chr8.4:9736031-9736050 MS.gene26688:CDS 40.0%
GCAATCCTTCAAAGGAAGAA+AGG - chr8.4:9735177-9735196 None:intergenic 40.0%
GCCTTTAATTATGATGCTGC+AGG + chr8.4:9736542-9736561 MS.gene26688:intron 40.0%
GGAGTACAAAACTGTATTCC+CGG - chr8.4:9737109-9737128 None:intergenic 40.0%
GTTGTGTACTCAAAGTATGC+AGG + chr8.4:9736260-9736279 MS.gene26688:CDS 40.0%
TAGAAATAATCCCATGGGAC+AGG - chr8.4:9735897-9735916 None:intergenic 40.0%
TATGCAGGGAATGACTTCAA+AGG + chr8.4:9737132-9737151 MS.gene26688:CDS 40.0%
TGGTATTCTTGAAACCGAAG+AGG + chr8.4:9736340-9736359 MS.gene26688:CDS 40.0%
! ATTGCTTTAGCTCAGGTCAT+TGG + chr8.4:9736923-9736942 MS.gene26688:intron 40.0%
! CGGCTTTAACAACAATAGAC+GGG - chr8.4:9735264-9735283 None:intergenic 40.0%
! CTACTCCTTTTGGATGTAAC+AGG + chr8.4:9737020-9737039 MS.gene26688:intron 40.0%
! GCGGCTTTAACAACAATAGA+CGG - chr8.4:9735265-9735284 None:intergenic 40.0%
! TTTAGCTCAGGTCATTGGTT+TGG + chr8.4:9736928-9736947 MS.gene26688:intron 40.0%
!! ATTGCCCTCTTTTAGAAGAG+TGG - chr8.4:9736696-9736715 None:intergenic 40.0%
AACTGTATTCCCGGGTGAAA+TGG - chr8.4:9737100-9737119 None:intergenic 45.0%
ACCAAGGATAAGCCATCCAT+TGG + chr8.4:9736615-9736634 MS.gene26688:CDS 45.0%
ACCAGTACTCTATCACCTAG+TGG - chr8.4:9736001-9736020 None:intergenic 45.0%
ACTGCTCAATCAAAGCCTCA+AGG + chr8.4:9736073-9736092 MS.gene26688:CDS 45.0%
AGAAAAGCTTACCGTGCCAA+TGG - chr8.4:9736634-9736653 None:intergenic 45.0%
ATACATAAGCCCTGTCCCAT+GGG + chr8.4:9735884-9735903 MS.gene26688:intron 45.0%
CATACAACTGTCAAGCCACT+AGG + chr8.4:9735983-9736002 MS.gene26688:CDS 45.0%
CTACAGCGAGAGATCAAAGT+AGG + chr8.4:9735619-9735638 MS.gene26688:intron 45.0%
CTGAAGGATGATGACATCGT+TGG + chr8.4:9736320-9736339 MS.gene26688:CDS 45.0%
CTGCTCAATCAAAGCCTCAA+GGG + chr8.4:9736074-9736093 MS.gene26688:CDS 45.0%
GAAGCTGAGGAAAAAACTGC+TGG + chr8.4:9736570-9736589 MS.gene26688:CDS 45.0%
GACAGTTGGGAAGAACAAAG+TGG + chr8.4:9736126-9736145 MS.gene26688:CDS 45.0%
GGAACTGAAGTGGAGTTCAA+TGG + chr8.4:9736281-9736300 MS.gene26688:CDS 45.0%
GGAAGTTCGCAATCCTTCAA+AGG - chr8.4:9735185-9735204 None:intergenic 45.0%
GGATGCAGAAGAGAAAACTC+AGG + chr8.4:9736030-9736049 MS.gene26688:CDS 45.0%
GGGATAATGCTAGTTGCTAC+AGG - chr8.4:9736851-9736870 None:intergenic 45.0%
TATACATAAGCCCTGTCCCA+TGG + chr8.4:9735883-9735902 MS.gene26688:intron 45.0%
TGCTCAATCAAAGCCTCAAG+GGG + chr8.4:9736075-9736094 MS.gene26688:CDS 45.0%
TTCCCGGGTGAAATGGATAA+TGG - chr8.4:9737093-9737112 None:intergenic 45.0%
TTTGAGAGCCTCAGATGTCA+TGG + chr8.4:9737179-9737198 MS.gene26688:CDS 45.0%
! GGCTTTGATTGAGCAGTTGA+TGG - chr8.4:9736070-9736089 None:intergenic 45.0%
! TGAGTGATGCCACTAGCTTA+TGG + chr8.4:9736164-9736183 MS.gene26688:intron 45.0%
!! CTGCTTCATCGATTTCTACG+AGG + chr8.4:9735149-9735168 MS.gene26688:CDS 45.0%
!!! GATCGAGTAGTTTTGGTCCT+AGG + chr8.4:9736981-9737000 MS.gene26688:intron 45.0%
AAGCTTACCGTGCCAATGGA+TGG - chr8.4:9736630-9736649 None:intergenic 50.0%
AGGTGATAGCTGTTGGTCCA+GGG + chr8.4:9737040-9737059 MS.gene26688:intron 50.0%
GAGGATAGCCATGACATCTG+AGG - chr8.4:9737190-9737209 None:intergenic 50.0%
GCAGAAGAGAAAACTCAGGG+TGG + chr8.4:9736034-9736053 MS.gene26688:CDS 50.0%
GCCAATGGATGGCTTATCCT+TGG - chr8.4:9736619-9736638 None:intergenic 50.0%
GCCACTAGGTGATAGAGTAC+TGG + chr8.4:9735997-9736016 MS.gene26688:CDS 50.0%
GCTCAATCAAAGCCTCAAGG+GGG + chr8.4:9736076-9736095 MS.gene26688:CDS 50.0%
GCTGAGGAAAAAACTGCTGG+TGG + chr8.4:9736573-9736592 MS.gene26688:CDS 50.0%
GGATAAGCCATCCATTGGCA+CGG + chr8.4:9736620-9736639 MS.gene26688:CDS 50.0%
TACCTTGGGGGTAACAACAG+TGG - chr8.4:9735287-9735306 None:intergenic 50.0%
TTGGAGACGGGAAGACAGTT+GGG + chr8.4:9736113-9736132 MS.gene26688:CDS 50.0%
! AGGTAGTTGCTGTTGGAGAC+GGG + chr8.4:9736101-9736120 MS.gene26688:CDS 50.0%
! GAGGTAGTTGCTGTTGGAGA+CGG + chr8.4:9736100-9736119 MS.gene26688:CDS 50.0%
!! TGCTGGTGGTTTGTTGCTCA+CGG + chr8.4:9736587-9736606 MS.gene26688:CDS 50.0%
ACTAAAGCTCCCGCATACGC+GGG + chr8.4:9735712-9735731 MS.gene26688:intron 55.0%
ATCAAAGCCTCAAGGGGGTG+AGG + chr8.4:9736081-9736100 MS.gene26688:CDS 55.0%
CACTAAAGCTCCCGCATACG+CGG + chr8.4:9735711-9735730 MS.gene26688:intron 55.0%
CAGGTGATAGCTGTTGGTCC+AGG + chr8.4:9737039-9737058 MS.gene26688:intron 55.0%
CTGTTGCCTTCGTCATCCAC+AGG - chr8.4:9737066-9737085 None:intergenic 55.0%
CTTGGGGGTAACAACAGTGG+CGG - chr8.4:9735284-9735303 None:intergenic 55.0%
GTTGCTCACGGAAGCAACCA+AGG + chr8.4:9736599-9736618 MS.gene26688:CDS 55.0%
GTTGGAGACGGGAAGACAGT+TGG + chr8.4:9736112-9736131 MS.gene26688:CDS 55.0%
! AAAATGGTGGGACCCCTTCC+CGG - chr8.4:9735740-9735759 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG - chr8.4:9735708-9735727 None:intergenic 55.0%
CGCCACTGTTGTTACCCCCA+AGG + chr8.4:9735282-9735301 MS.gene26688:CDS 60.0%
CTAAAGCTCCCGCATACGCG+GGG + chr8.4:9735713-9735732 MS.gene26688:intron 60.0%
GCAACTACCTCACCCCCTTG+AGG - chr8.4:9736091-9736110 None:intergenic 60.0%
GCTGCAGGTTGCAGAAGCTG+AGG + chr8.4:9736557-9736576 MS.gene26688:intron 60.0%
GGGGGTGAGGTAGTTGCTGT+TGG + chr8.4:9736094-9736113 MS.gene26688:CDS 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG - chr8.4:9735707-9735726 None:intergenic 60.0%
AGCTGTTGGTCCAGGGCCTG+TGG + chr8.4:9737047-9737066 MS.gene26688:CDS 65.0%
CCAGGGCCTGTGGATGACGA+AGG + chr8.4:9737057-9737076 MS.gene26688:CDS 65.0%
CCTTCGTCATCCACAGGCCC+TGG - chr8.4:9737060-9737079 None:intergenic 65.0%
CATACGCGGGGTCCGGGAAG+GGG + chr8.4:9735725-9735744 MS.gene26688:intron 70.0%
CTTCCCGGACCCCGCGTATG+CGG - chr8.4:9735725-9735744 None:intergenic 70.0%
GCATACGCGGGGTCCGGGAA+GGG + chr8.4:9735724-9735743 MS.gene26688:intron 70.0%
TTCCCGGACCCCGCGTATGC+GGG - chr8.4:9735724-9735743 None:intergenic 70.0%
CGCATACGCGGGGTCCGGGA+AGG + chr8.4:9735723-9735742 MS.gene26688:intron 75.0%
CTCCCGCATACGCGGGGTCC+GGG + chr8.4:9735719-9735738 MS.gene26688:intron 75.0%
GCTCCCGCATACGCGGGGTC+CGG + chr8.4:9735718-9735737 MS.gene26688:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 9735130 9737215 9735130 ID=MS.gene26688
chr8.4 mRNA 9735130 9737215 9735130 ID=MS.gene26688.t1;Parent=MS.gene26688
chr8.4 exon 9735130 9735303 9735130 ID=MS.gene26688.t1.exon1;Parent=MS.gene26688.t1
chr8.4 CDS 9735130 9735303 9735130 ID=cds.MS.gene26688.t1;Parent=MS.gene26688.t1
chr8.4 exon 9735983 9736162 9735983 ID=MS.gene26688.t1.exon2;Parent=MS.gene26688.t1
chr8.4 CDS 9735983 9736162 9735983 ID=cds.MS.gene26688.t1;Parent=MS.gene26688.t1
chr8.4 exon 9736248 9736403 9736248 ID=MS.gene26688.t1.exon3;Parent=MS.gene26688.t1
chr8.4 CDS 9736248 9736403 9736248 ID=cds.MS.gene26688.t1;Parent=MS.gene26688.t1
chr8.4 exon 9736564 9736641 9736564 ID=MS.gene26688.t1.exon4;Parent=MS.gene26688.t1
chr8.4 CDS 9736564 9736641 9736564 ID=cds.MS.gene26688.t1;Parent=MS.gene26688.t1
chr8.4 exon 9737042 9737215 9737042 ID=MS.gene26688.t1.exon5;Parent=MS.gene26688.t1
chr8.4 CDS 9737042 9737215 9737042 ID=cds.MS.gene26688.t1;Parent=MS.gene26688.t1
Gene Sequence

>MS.gene26688

ATGGCGACTACTCAGCTTACTGCTTCATCGATTTCTACGAGGAACCTTTCTTCCTTTGAAGGATTGCGAACTTCCGCAATTCATTTTCCTCGTAACAATGCTAGAATTGGTATTCTTACACAAAGGTCGTTCCCGTCTATTGTTGTTAAAGCCGCCACTGTTGTTACCCCCAAGGTATTATTTTTATTTTTTGGGTCATGGATTTTCTTCATTATCTGCGGCAAAATTATTCTTTTCAGTAGTATATTATGATAAAATAGAGTTAAACTGTTTTTGTATATGCTGCTATAAGTTGTGTTTATAAGCTATCTTGGAGAACTTGCGAAATTAAGTTGAGAAACACTTATGAAAAGTGCCATAAGCTATAAGCTCTTTCAAACACCCACTCAAGTGCATTATGAGATAGTTATCACACAAGTGCATTATCTTACGAGTACCCCTTAGTATAAGAGAAAATGTACAATTTGTGAGTAGATAGAGAAAGCGAAGCTACAGCGAGAGATCAAAGTAGGAGTTAGAAAGAGTGAAAAATTCATTTGCAACTAAATTTCAGACCAATAAAAAAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATACGCGGGGTCCGGGAAGGGGTCCCACCATTTTTGAATATTTTTCAGACCAATATTTATGAATATTTTTCGTACAGAAGAACATTATCTTTTTATTTCGTAAGCGAAATAGCAGACTATTGATATACATTTCATCTTGAATTAACATGTTCATTGATATACATAAGCCCTGTCCCATGGGATTATTTCTAGTGTTTTTACTTCTACATTTTACAATTCAATGCTGCATGCGCCTTATTAGTAATTTCATCTTGTAGCATACAACTGTCAAGCCACTAGGTGATAGAGTACTGGTAAAAATCAAGGATGCAGAAGAGAAAACTCAGGGTGGAATTTTGCTGCCATCAACTGCTCAATCAAAGCCTCAAGGGGGTGAGGTAGTTGCTGTTGGAGACGGGAAGACAGTTGGGAAGAACAAAGTGGACATTAGTGTGAAGGTGAGTGATGCCACTAGCTTATGGAATCCTTTAAGCCTTGGTCTTTCTTTTATTTTGGATTTATGTAATGTATTTCTCTTTACAGGCAGGTGCACAAGTTGTGTACTCAAAGTATGCAGGAACTGAAGTGGAGTTCAATGGTTCAAAACATCTTATACTGAAGGATGATGACATCGTTGGTATTCTTGAAACCGAAGAGGTCAAAGATCTTAAACCCTTGAATGACAGAGTCTTGATAAAGGTGTGGAACTTTTCTGAGATTTGATTATTTCGATGCTTCAGTTGTCTTGATGATCTGCCCATTTAATTATTTGTCTTAACCATATTTTGCATTAAGGTGTTACGTTTAAAGGCAATATTATATCTGAATATTATACATGCCTTTAATTATGATGCTGCAGGTTGCAGAAGCTGAGGAAAAAACTGCTGGTGGTTTGTTGCTCACGGAAGCAACCAAGGATAAGCCATCCATTGGCACGGTAAGCTTTTCTATTCTCCTTTTTTTGAAACAGTATACTTAAAAAGTAACACCACTCTTCTAAAAGAGGGCAATGTTATATCGTATGAAGGGAAGGTCACAAGAATTGCAAGAATGTGATTTGGGCTGTTTTCAAAAAATTAAAACAATGGATGGTCTGGTGAAAATGAACGCATTAGATACTGTCGTGCATTTAATTTTTGTGTTCCTGTAGCAACTAGCATTATCCCTTAAGACGGTAGAATGTGTTGATGACAGTTTAACTGATGCTTATTTTTATTTATTGCTTTAGCTCAGGTCATTGGTTTGGTTCTTACTTGCTTAGCTGTAACATTGTTTTGATCGAGTAGTTTTGGTCCTAGGTATGTAAATAACCATTCTACTCCTTTTGGATGTAACAGGTGATAGCTGTTGGTCCAGGGCCTGTGGATGACGAAGGCAACAGAAAACCATTATCCATTTCACCCGGGAATACAGTTTTGTACTCCAAATATGCAGGGAATGACTTCAAAGGCAAAGATGGTTCTGACTATATTGCTTTGAGAGCCTCAGATGTCATGGCTATCCTCTCTTAA
Protein sequence

>MS.gene26688.t1

MATTQLTASSISTRNLSSFEGLRTSAIHFPRNNARIGILTQRSFPSIVVKAATVVTPKHTTVKPLGDRVLVKIKDAEEKTQGGILLPSTAQSKPQGGEVVAVGDGKTVGKNKVDISVKAGAQVVYSKYAGTEVEFNGSKHLILKDDDIVGILETEEVKDLKPLNDRVLIKVAEAEEKTAGGLLLTEATKDKPSIGTVIAVGPGPVDDEGNRKPLSISPGNTVLYSKYAGNDFKGKDGSDYIALRASDVMAILS