Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26960.t1 | XP_013466098.1 | 96.5 | 457 | 16 | 0 | 1 | 457 | 1 | 457 | 5.10E-246 | 860.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26960.t1 | Q9LH84 | 51.3 | 466 | 213 | 4 | 4 | 465 | 7 | 462 | 6.9e-127 | 455.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26960.t1 | A0A072VE89 | 96.5 | 457 | 16 | 0 | 1 | 457 | 1 | 457 | 3.7e-246 | 860.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene26960 | 0.803228 | 3.85E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene26960 | 0.811321 | 7.42E-51 | -1.69E-46 |
| MS.gene049452 | MS.gene26960 | 0.807319 | 5.36E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene26960 | 0.802149 | 6.44E-49 | -1.69E-46 |
| MS.gene050759 | MS.gene26960 | 0.808079 | 3.69E-50 | -1.69E-46 |
| MS.gene050962 | MS.gene26960 | 0.801738 | 7.81E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene26960 | 0.803861 | 2.85E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene26960 | 0.82384 | 1.12E-53 | -1.69E-46 |
| MS.gene051490 | MS.gene26960 | 0.807664 | 4.53E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene26960 | 0.847827 | 9.04E-60 | -1.69E-46 |
| MS.gene051800 | MS.gene26960 | 0.80197 | 7.00E-49 | -1.69E-46 |
| MS.gene052016 | MS.gene26960 | 0.802751 | 4.84E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene26960 | 0.812373 | 4.38E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene26960 | 0.809559 | 1.78E-50 | -1.69E-46 |
| MS.gene052584 | MS.gene26960 | 0.822971 | 1.78E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene26960 | 0.830074 | 3.60E-55 | -1.69E-46 |
| MS.gene053296 | MS.gene26960 | 0.925421 | 1.88E-90 | -1.69E-46 |
| MS.gene053621 | MS.gene26960 | 0.803632 | 3.18E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene26960 | 0.829392 | 5.28E-55 | -1.69E-46 |
| MS.gene054576 | MS.gene26960 | 0.804192 | 2.43E-49 | -1.69E-46 |
| MS.gene05458 | MS.gene26960 | 0.803372 | 3.60E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene26960 | 0.834449 | 2.98E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene26960 | 0.824581 | 7.47E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene26960 | 0.832396 | 9.68E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene26960 | 0.801129 | 1.04E-48 | -1.69E-46 |
| MS.gene056050 | MS.gene26960 | 0.813106 | 3.03E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene26960 | 0.818326 | 2.08E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene26960 | 0.819811 | 9.55E-53 | -1.69E-46 |
| MS.gene057034 | MS.gene26960 | 0.815913 | 7.25E-52 | -1.69E-46 |
| MS.gene057915 | MS.gene26960 | 0.819414 | 1.18E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene26960 | 0.82355 | 1.30E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene26960 | 0.806492 | 8.01E-50 | -1.69E-46 |
| MS.gene059570 | MS.gene26960 | 0.804791 | 1.82E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26960.t1 | MTR_1g022245 | 97.194 | 499 | 14 | 0 | 1 | 499 | 1 | 499 | 0.0 | 1009 |
| MS.gene26960.t1 | MTR_2g030420 | 54.393 | 478 | 195 | 4 | 3 | 477 | 8 | 465 | 0.0 | 537 |
| MS.gene26960.t1 | MTR_4g107370 | 53.074 | 488 | 221 | 4 | 1 | 483 | 6 | 490 | 0.0 | 535 |
| MS.gene26960.t1 | MTR_1g022250 | 55.556 | 477 | 194 | 4 | 3 | 476 | 5 | 466 | 0.0 | 534 |
| MS.gene26960.t1 | MTR_6g009540 | 52.000 | 525 | 216 | 10 | 3 | 511 | 5 | 509 | 1.65e-179 | 514 |
| MS.gene26960.t1 | MTR_7g008040 | 53.219 | 466 | 205 | 5 | 1 | 460 | 1 | 459 | 1.87e-175 | 505 |
| MS.gene26960.t1 | MTR_6g009520 | 48.381 | 525 | 240 | 7 | 3 | 511 | 7 | 516 | 6.44e-168 | 485 |
| MS.gene26960.t1 | MTR_6g009553 | 53.556 | 450 | 196 | 7 | 21 | 460 | 1 | 447 | 1.38e-164 | 475 |
| MS.gene26960.t1 | MTR_6g009720 | 51.717 | 466 | 213 | 5 | 3 | 460 | 7 | 468 | 1.79e-164 | 476 |
| MS.gene26960.t1 | MTR_6g009770 | 51.178 | 467 | 192 | 9 | 3 | 460 | 7 | 446 | 1.67e-154 | 450 |
| MS.gene26960.t1 | MTR_6g042290 | 47.930 | 459 | 206 | 5 | 7 | 460 | 20 | 450 | 5.78e-149 | 436 |
| MS.gene26960.t1 | MTR_6g009510 | 45.691 | 499 | 182 | 11 | 3 | 489 | 20 | 441 | 9.71e-137 | 403 |
| MS.gene26960.t1 | MTR_6g009660 | 42.857 | 518 | 217 | 11 | 4 | 497 | 5 | 467 | 3.78e-127 | 379 |
| MS.gene26960.t1 | MTR_5g020980 | 37.378 | 511 | 260 | 10 | 4 | 491 | 13 | 486 | 1.88e-108 | 332 |
| MS.gene26960.t1 | MTR_4g128260 | 36.210 | 533 | 295 | 11 | 1 | 506 | 1 | 515 | 1.92e-106 | 328 |
| MS.gene26960.t1 | MTR_2g076970 | 38.645 | 502 | 252 | 14 | 1 | 491 | 1 | 457 | 1.01e-105 | 324 |
| MS.gene26960.t1 | MTR_2g061820 | 38.242 | 455 | 245 | 10 | 8 | 449 | 3 | 434 | 2.52e-103 | 320 |
| MS.gene26960.t1 | MTR_2g076920 | 37.426 | 505 | 263 | 10 | 1 | 496 | 7 | 467 | 4.09e-102 | 315 |
| MS.gene26960.t1 | MTR_2g076690 | 40.647 | 433 | 208 | 8 | 61 | 484 | 65 | 457 | 5.31e-102 | 314 |
| MS.gene26960.t1 | MTR_2g076710 | 37.684 | 475 | 247 | 10 | 1 | 464 | 5 | 441 | 6.50e-100 | 308 |
| MS.gene26960.t1 | MTR_2g076990 | 39.450 | 436 | 217 | 8 | 61 | 487 | 1 | 398 | 9.19e-97 | 300 |
| MS.gene26960.t1 | MTR_2g076840 | 37.029 | 478 | 245 | 10 | 1 | 466 | 1 | 434 | 4.71e-96 | 306 |
| MS.gene26960.t1 | MTR_2g076840 | 45.522 | 268 | 116 | 4 | 195 | 462 | 467 | 704 | 2.66e-72 | 244 |
| MS.gene26960.t1 | MTR_8g076920 | 35.352 | 512 | 275 | 11 | 9 | 507 | 28 | 496 | 2.69e-94 | 295 |
| MS.gene26960.t1 | MTR_2g076900 | 35.800 | 500 | 260 | 12 | 1 | 484 | 1 | 455 | 4.36e-92 | 288 |
| MS.gene26960.t1 | MTR_5g020990 | 37.410 | 417 | 213 | 10 | 63 | 461 | 84 | 470 | 3.17e-87 | 276 |
| MS.gene26960.t1 | MTR_3g103150 | 42.581 | 310 | 139 | 6 | 154 | 461 | 4 | 276 | 3.61e-78 | 246 |
| MS.gene26960.t1 | MTR_1g011680 | 68.675 | 166 | 45 | 3 | 335 | 499 | 7 | 166 | 4.52e-63 | 204 |
| MS.gene26960.t1 | MTR_7g117950 | 31.477 | 413 | 213 | 11 | 57 | 462 | 57 | 406 | 3.17e-60 | 206 |
| MS.gene26960.t1 | MTR_3g092060 | 32.216 | 388 | 202 | 8 | 61 | 441 | 63 | 396 | 3.76e-58 | 201 |
| MS.gene26960.t1 | MTR_5g021020 | 35.583 | 326 | 153 | 8 | 138 | 461 | 135 | 405 | 5.60e-56 | 192 |
| MS.gene26960.t1 | MTR_8g027200 | 28.059 | 474 | 239 | 13 | 35 | 501 | 34 | 412 | 4.30e-48 | 172 |
| MS.gene26960.t1 | MTR_1g113750 | 67.200 | 125 | 22 | 2 | 389 | 509 | 16 | 125 | 8.51e-45 | 154 |
| MS.gene26960.t1 | MTR_5g020720 | 36.464 | 181 | 84 | 5 | 259 | 437 | 25 | 176 | 2.85e-27 | 108 |
| MS.gene26960.t1 | MTR_5g021000 | 50.000 | 108 | 49 | 2 | 356 | 461 | 1 | 105 | 1.04e-26 | 106 |
| MS.gene26960.t1 | MTR_1g008630 | 64.198 | 81 | 8 | 1 | 405 | 485 | 1 | 60 | 2.10e-22 | 91.7 |
| MS.gene26960.t1 | MTR_5g021010 | 48.352 | 91 | 42 | 2 | 373 | 461 | 34 | 121 | 1.35e-18 | 82.0 |
| MS.gene26960.t1 | MTR_2g076820 | 53.846 | 52 | 24 | 0 | 389 | 440 | 1 | 52 | 2.26e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26960.t1 | AT3G28510 | 50.427 | 468 | 218 | 4 | 4 | 467 | 7 | 464 | 1.25e-165 | 479 |
| MS.gene26960.t1 | AT3G28580 | 50.000 | 462 | 216 | 6 | 1 | 457 | 4 | 455 | 2.18e-157 | 457 |
| MS.gene26960.t1 | AT3G28540 | 49.679 | 467 | 215 | 7 | 1 | 459 | 1 | 455 | 4.91e-155 | 452 |
| MS.gene26960.t1 | AT3G28540 | 49.465 | 467 | 216 | 7 | 1 | 459 | 1 | 455 | 5.31e-155 | 452 |
| MS.gene26960.t1 | AT5G40010 | 47.885 | 520 | 247 | 7 | 1 | 511 | 4 | 508 | 5.27e-152 | 444 |
| MS.gene26960.t1 | AT3G28600 | 50.316 | 475 | 188 | 11 | 8 | 465 | 7 | 450 | 3.51e-150 | 438 |
| MS.gene26960.t1 | AT3G28610 | 49.790 | 476 | 192 | 12 | 7 | 465 | 5 | 450 | 5.31e-149 | 435 |
| MS.gene26960.t1 | AT5G40000 | 47.808 | 479 | 197 | 9 | 1 | 460 | 4 | 448 | 1.08e-144 | 424 |
| MS.gene26960.t1 | AT3G28580 | 46.753 | 462 | 209 | 7 | 1 | 457 | 4 | 433 | 5.14e-140 | 412 |
| MS.gene26960.t1 | AT3G28570 | 46.791 | 483 | 200 | 9 | 8 | 476 | 9 | 448 | 2.80e-138 | 407 |
| MS.gene26960.t1 | AT3G28570 | 46.791 | 483 | 200 | 9 | 8 | 476 | 16 | 455 | 4.83e-138 | 406 |
| MS.gene26960.t1 | AT3G28520 | 46.421 | 489 | 226 | 7 | 12 | 480 | 1 | 473 | 1.15e-134 | 399 |
| MS.gene26960.t1 | AT3G28600 | 46.947 | 475 | 165 | 10 | 8 | 465 | 7 | 411 | 1.41e-133 | 394 |
| MS.gene26960.t1 | AT4G25835 | 38.586 | 495 | 271 | 10 | 1 | 483 | 1 | 474 | 1.13e-109 | 335 |
| MS.gene26960.t1 | AT5G57480 | 36.893 | 515 | 289 | 11 | 1 | 502 | 1 | 492 | 6.82e-108 | 331 |
| MS.gene26960.t1 | AT3G50940 | 40.173 | 463 | 224 | 11 | 20 | 471 | 31 | 451 | 4.93e-105 | 322 |
| MS.gene26960.t1 | AT2G18193 | 38.689 | 473 | 237 | 12 | 15 | 477 | 25 | 454 | 1.55e-102 | 317 |
| MS.gene26960.t1 | AT3G50930 | 39.050 | 484 | 252 | 9 | 16 | 485 | 73 | 527 | 4.02e-101 | 316 |
| MS.gene26960.t1 | AT5G17740 | 36.779 | 503 | 270 | 12 | 4 | 494 | 13 | 479 | 6.45e-97 | 303 |
| MS.gene26960.t1 | AT4G30250 | 37.395 | 476 | 266 | 7 | 1 | 461 | 1 | 459 | 1.91e-94 | 296 |
| MS.gene26960.t1 | AT4G30250 | 37.395 | 476 | 266 | 7 | 1 | 461 | 3 | 461 | 2.47e-94 | 296 |
| MS.gene26960.t1 | AT2G18190 | 36.287 | 474 | 247 | 11 | 15 | 476 | 26 | 456 | 7.49e-94 | 294 |
| MS.gene26960.t1 | AT5G17730 | 37.108 | 415 | 215 | 6 | 64 | 468 | 72 | 450 | 4.83e-90 | 283 |
| MS.gene26960.t1 | AT5G17760 | 36.807 | 451 | 225 | 10 | 64 | 490 | 72 | 486 | 7.38e-89 | 281 |
| MS.gene26960.t1 | AT4G05380 | 35.586 | 444 | 230 | 9 | 48 | 468 | 44 | 454 | 1.16e-86 | 275 |
| MS.gene26960.t1 | AT5G17750 | 37.958 | 382 | 188 | 6 | 64 | 427 | 41 | 391 | 3.63e-79 | 253 |
| MS.gene26960.t1 | AT1G43910 | 37.297 | 370 | 187 | 6 | 103 | 464 | 118 | 450 | 2.56e-78 | 253 |
| MS.gene26960.t1 | AT2G46620 | 32.319 | 427 | 224 | 9 | 45 | 462 | 47 | 417 | 7.27e-65 | 218 |
| MS.gene26960.t1 | AT3G29800 | 31.095 | 402 | 207 | 10 | 64 | 458 | 39 | 377 | 1.36e-51 | 182 |
| MS.gene26960.t1 | AT5G17760 | 34.749 | 259 | 144 | 4 | 64 | 305 | 72 | 322 | 4.02e-49 | 172 |
| MS.gene26960.t1 | AT3G28560 | 40.741 | 216 | 113 | 6 | 4 | 212 | 7 | 214 | 4.30e-43 | 154 |
| MS.gene26960.t1 | AT4G05340 | 53.165 | 79 | 37 | 0 | 335 | 413 | 15 | 93 | 2.99e-20 | 85.9 |
Find 81 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAGTCAGGTAACACTTTC+TGG | 0.123714 | 1.3:-2825388 | MS.gene26960:CDS |
| TTGCAAGCTTAATGTTTATA+TGG | 0.183844 | 1.3:-2826383 | MS.gene26960:CDS |
| ACCAAACATGTTTGAACTTT+GGG | 0.187189 | 1.3:-2826424 | None:intergenic |
| AAGAGAGGCTATTTGCTTTA+TGG | 0.209267 | 1.3:-2825712 | MS.gene26960:CDS |
| CATCTCAGCCTCTTCCTTAG+AGG | 0.245609 | 1.3:+2825033 | None:intergenic |
| TGAATTTCATTGATGGGATT+TGG | 0.272209 | 1.3:-2825360 | MS.gene26960:CDS |
| TTTGAACTTTGGGGTCTAAT+AGG | 0.288966 | 1.3:-2826414 | MS.gene26960:CDS |
| CACCTTGAAGTTTCTTTGCT+TGG | 0.291226 | 1.3:+2826178 | None:intergenic |
| ATCTTACCTTAACCATGTTT+TGG | 0.294915 | 1.3:-2825962 | MS.gene26960:CDS |
| AGGAGATTGGTTTGAGTAAG+AGG | 0.309573 | 1.3:-2825930 | MS.gene26960:CDS |
| AATGAAACAGAATTTGTGTT+AGG | 0.312689 | 1.3:+2826054 | None:intergenic |
| TTGAAGGGGTTAAGTTTGTT+TGG | 0.318297 | 1.3:-2826095 | MS.gene26960:CDS |
| AACCAAACATGTTTGAACTT+TGG | 0.318708 | 1.3:-2826425 | None:intergenic |
| AATGTATAAGAAAGCTTCAT+TGG | 0.320305 | 1.3:+2826230 | None:intergenic |
| CCTTAGAGGTTCTAAGTGCT+TGG | 0.356753 | 1.3:+2825047 | None:intergenic |
| GATTGTTCGCTTGATCTTAC+CGG | 0.367967 | 1.3:-2825529 | MS.gene26960:CDS |
| AGTTGCTGCTGCTTCTCTGC+AGG | 0.371093 | 1.3:-2825443 | MS.gene26960:CDS |
| CTTTATGGTCCTCCTGGAAC+AGG | 0.377541 | 1.3:-2825697 | MS.gene26960:CDS |
| ATCCTTTGTTCGAGATGGTT+CGG | 0.385486 | 1.3:-2825171 | MS.gene26960:CDS |
| CCAAGCACTTAGAACCTCTA+AGG | 0.388467 | 1.3:-2825047 | MS.gene26960:CDS |
| AAGAATCTAACCTCAACAAA+AGG | 0.394204 | 1.3:+2826279 | None:intergenic |
| TGCAAGCTTAATGTTTATAT+GGG | 0.401015 | 1.3:-2826382 | MS.gene26960:CDS |
| TTGAAGTTTCTTTGCTTGGT+TGG | 0.404145 | 1.3:+2826182 | None:intergenic |
| TTGGAACAAGGTAAGGAGAT+TGG | 0.405159 | 1.3:-2825943 | MS.gene26960:CDS |
| TACACAAATTGCACAGGTAA+TGG | 0.421924 | 1.3:-2825895 | MS.gene26960:CDS |
| GGGTTATTGAATTTCATTGA+TGG | 0.427942 | 1.3:-2825367 | MS.gene26960:CDS |
| AGCAAAGAACAAAGCAAGTC+AGG | 0.436198 | 1.3:-2825401 | MS.gene26960:CDS |
| CAAGTCAGGTAACACTTTCT+GGG | 0.437874 | 1.3:-2825387 | MS.gene26960:CDS |
| TCATTGGTATTCTCTTCCTT+AGG | 0.447944 | 1.3:+2824911 | None:intergenic |
| CGAGATGGTTCGGTCCTTGT+TGG | 0.450554 | 1.3:-2825161 | MS.gene26960:CDS |
| ATCTCATCCTTTGTTCGAGA+TGG | 0.457463 | 1.3:-2825176 | MS.gene26960:CDS |
| AACAAAAGGGTAACAAAAGT+TGG | 0.459264 | 1.3:+2826293 | None:intergenic |
| ATGAAACAGAATTTGTGTTA+GGG | 0.466214 | 1.3:+2826055 | None:intergenic |
| AAAGTCTTTAGTTCTAAAGA+TGG | 0.468242 | 1.3:-2826145 | MS.gene26960:CDS |
| TATTTGCTTTATGGTCCTCC+TGG | 0.473621 | 1.3:-2825703 | MS.gene26960:CDS |
| AACCAAGCAAAGAAACTTCA+AGG | 0.474512 | 1.3:-2826180 | MS.gene26960:CDS |
| CCTTACCTTGTTCCAAAACA+TGG | 0.476787 | 1.3:+2825950 | None:intergenic |
| CATATTGAGTTGTCTTATTG+TGG | 0.480153 | 1.3:-2825244 | MS.gene26960:CDS |
| GGTTATTGAATTTCATTGAT+GGG | 0.481156 | 1.3:-2825366 | MS.gene26960:CDS |
| GTTGCTGCTGCTTCTCTGCA+GGG | 0.482716 | 1.3:-2825442 | MS.gene26960:CDS |
| CTTGTTCCAAAACATGGTTA+AGG | 0.486598 | 1.3:+2825956 | None:intergenic |
| TGATCTTGTTACATTCAGTA+AGG | 0.490186 | 1.3:-2825770 | MS.gene26960:CDS |
| GGGATTTGGTCTGCTAGTAC+TGG | 0.502830 | 1.3:-2825346 | MS.gene26960:CDS |
| AGAAGAAGGGACAGGTGAAG+AGG | 0.504002 | 1.3:-2824975 | MS.gene26960:CDS |
| TAAGAAAGAAGCAGAGATGA+AGG | 0.504395 | 1.3:-2825005 | MS.gene26960:CDS |
| GGAGAATGACAGAGAAAAGA+AGG | 0.505958 | 1.3:-2825488 | MS.gene26960:CDS |
| GAATACCAATGAAAATTCCT+AGG | 0.507426 | 1.3:-2824900 | None:intergenic |
| GCAATTGTCTCAAAAGAAGA+TGG | 0.507729 | 1.3:+2825826 | None:intergenic |
| GTTAGATTCTTTGAGAACAT+TGG | 0.509702 | 1.3:-2826267 | MS.gene26960:CDS |
| CAGGTAATGGTGAATATGAA+AGG | 0.510294 | 1.3:-2825882 | MS.gene26960:CDS |
| ATGAAGGCTGAGAAAGAAGA+AGG | 0.511615 | 1.3:-2824989 | MS.gene26960:CDS |
| CGGTCAGAGGAAGACAGATT+CGG | 0.513344 | 1.3:-2825509 | MS.gene26960:CDS |
| CCAAACATGTTTGAACTTTG+GGG | 0.515294 | 1.3:-2826423 | None:intergenic |
| AGCAAATAGCCTCTCTTCCA+AGG | 0.530468 | 1.3:+2825718 | None:intergenic |
| TTTGATTCGTCGTGGACGAA+TGG | 0.533266 | 1.3:-2825272 | MS.gene26960:CDS |
| AGGTAATGGTGAATATGAAA+GGG | 0.535037 | 1.3:-2825881 | MS.gene26960:CDS |
| AGAATCTAACCTCAACAAAA+GGG | 0.545291 | 1.3:+2826280 | None:intergenic |
| CGCTTGATCTTACCGGTCAG+AGG | 0.554416 | 1.3:-2825522 | MS.gene26960:CDS |
| GACCGAACCATCTCGAACAA+AGG | 0.555408 | 1.3:+2825169 | None:intergenic |
| ATTGGTAAGCCTTGGAAGAG+AGG | 0.555511 | 1.3:-2825727 | MS.gene26960:CDS |
| TGAAGAGGATTCATCAGAGA+AGG | 0.557487 | 1.3:-2824960 | MS.gene26960:CDS |
| CGAATCTGTCTTCCTCTGAC+CGG | 0.558778 | 1.3:+2825510 | None:intergenic |
| ACTTAGAACCTCTAAGGAAG+AGG | 0.569363 | 1.3:-2825041 | MS.gene26960:CDS |
| GCTGAGAAAGAAGAAGGGAC+AGG | 0.571855 | 1.3:-2824983 | MS.gene26960:CDS |
| AAGCTTTACACAAATTGCAC+AGG | 0.572705 | 1.3:-2825901 | MS.gene26960:CDS |
| ACATCTTCATCAGCTACCTT+AGG | 0.574849 | 1.3:+2825094 | None:intergenic |
| TATATTCACTACTAACTATG+TGG | 0.586952 | 1.3:-2825311 | MS.gene26960:CDS |
| TGCTGAAAACTTGATGCCTA+AGG | 0.589054 | 1.3:-2825110 | MS.gene26960:CDS |
| AATATGAAAGGGGAGGTAAG+TGG | 0.596088 | 1.3:-2825870 | MS.gene26960:CDS |
| TGCAGAAGACAAGAAGCCTA+AGG | 0.596943 | 1.3:-2824927 | MS.gene26960:CDS |
| AGAGGATTCATCAGAGAAGG+AGG | 0.600261 | 1.3:-2824957 | MS.gene26960:CDS |
| GGTAGCTGATGAAGATGTTG+AGG | 0.602813 | 1.3:-2825089 | MS.gene26960:CDS |
| CATGTTAGTCTCTTCCAACA+AGG | 0.626039 | 1.3:+2825147 | None:intergenic |
| TGAAGGCTGAGAAAGAAGAA+GGG | 0.627565 | 1.3:-2824988 | MS.gene26960:CDS |
| AATCACGAGTAAATCTGTTG+TGG | 0.629066 | 1.3:-2825566 | MS.gene26960:CDS |
| GATCAAGCTTTGATTCGTCG+TGG | 0.629566 | 1.3:-2825280 | MS.gene26960:CDS |
| ATGTTGAGGCATCATTAGAG+AGG | 0.635432 | 1.3:-2825075 | MS.gene26960:CDS |
| AATGGTGAATATGAAAGGGG+AGG | 0.665295 | 1.3:-2825877 | MS.gene26960:CDS |
| GGTAATGGTGAATATGAAAG+GGG | 0.666341 | 1.3:-2825880 | MS.gene26960:CDS |
| ATGAGTGCATGAACTTGATG+AGG | 0.669050 | 1.3:+2826339 | None:intergenic |
| GACTGCGATGAAATGTGAGA+AGG | 0.695268 | 1.3:+2826004 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAGTATTATGCAAAAAT+TGG | - | chr1.3:2825573-2825592 | MS.gene26960:CDS | 20.0% |
| ! | AATGAAACAGAATTTGTGTT+AGG | + | chr1.3:2825267-2825286 | None:intergenic | 25.0% |
| ! | AATGTATAAGAAAGCTTCAT+TGG | + | chr1.3:2825091-2825110 | None:intergenic | 25.0% |
| ! | ATGAAACAGAATTTGTGTTA+GGG | + | chr1.3:2825266-2825285 | None:intergenic | 25.0% |
| ! | GGTTATTGAATTTCATTGAT+GGG | - | chr1.3:2825952-2825971 | MS.gene26960:CDS | 25.0% |
| ! | TATATTCACTACTAACTATG+TGG | - | chr1.3:2826007-2826026 | MS.gene26960:CDS | 25.0% |
| ! | TGAAAAATAATGCTGAGTTA+AGG | - | chr1.3:2825715-2825734 | MS.gene26960:CDS | 25.0% |
| ! | TGCAAGCTTAATGTTTATAT+GGG | - | chr1.3:2824936-2824955 | MS.gene26960:CDS | 25.0% |
| ! | TTGCAAGCTTAATGTTTATA+TGG | - | chr1.3:2824935-2824954 | MS.gene26960:CDS | 25.0% |
| !! | ACAATCTCTTTCTTCTTTTT+AGG | + | chr1.3:2825525-2825544 | None:intergenic | 25.0% |
| !!! | AAAGTCTTTAGTTCTAAAGA+TGG | - | chr1.3:2825173-2825192 | MS.gene26960:CDS | 25.0% |
| !!! | AAGTTTTATGATGAGTTTGA+AGG | - | chr1.3:2825207-2825226 | MS.gene26960:CDS | 25.0% |
| !!! | AGTTTTATGATGAGTTTGAA+GGG | - | chr1.3:2825208-2825227 | MS.gene26960:CDS | 25.0% |
| AACAAAAGGGTAACAAAAGT+TGG | + | chr1.3:2825028-2825047 | None:intergenic | 30.0% | |
| AAGAATCTAACCTCAACAAA+AGG | + | chr1.3:2825042-2825061 | None:intergenic | 30.0% | |
| AGAAAAGAAGGAGAAAAATG+AGG | - | chr1.3:2825842-2825861 | MS.gene26960:CDS | 30.0% | |
| AGAATCTAACCTCAACAAAA+GGG | + | chr1.3:2825041-2825060 | None:intergenic | 30.0% | |
| GGGTTATTGAATTTCATTGA+TGG | - | chr1.3:2825951-2825970 | MS.gene26960:CDS | 30.0% | |
| TGAATTTCATTGATGGGATT+TGG | - | chr1.3:2825958-2825977 | MS.gene26960:CDS | 30.0% | |
| TGATCTTGTTACATTCAGTA+AGG | - | chr1.3:2825548-2825567 | MS.gene26960:CDS | 30.0% | |
| ! | AGGTAATGGTGAATATGAAA+GGG | - | chr1.3:2825437-2825456 | MS.gene26960:CDS | 30.0% |
| ! | ATCTTACCTTAACCATGTTT+TGG | - | chr1.3:2825356-2825375 | MS.gene26960:CDS | 30.0% |
| ! | CATATTGAGTTGTCTTATTG+TGG | - | chr1.3:2826074-2826093 | MS.gene26960:CDS | 30.0% |
| ! | GAGTTAAGGAAACTTTTGAT+TGG | - | chr1.3:2825729-2825748 | MS.gene26960:CDS | 30.0% |
| ! | GTTAGATTCTTTGAGAACAT+TGG | - | chr1.3:2825051-2825070 | MS.gene26960:CDS | 30.0% |
| ! | GTTTTATGATGAGTTTGAAG+GGG | - | chr1.3:2825209-2825228 | MS.gene26960:CDS | 30.0% |
| !! | CTCAGCATTATTTTTCACAT+TGG | + | chr1.3:2825712-2825731 | None:intergenic | 30.0% |
| !!! | TTGTCTTATTGTGGTTTTGA+TGG | - | chr1.3:2826083-2826102 | MS.gene26960:CDS | 30.0% |
| !!! | TTTTGATGGATTCAAAATGC+TGG | - | chr1.3:2826097-2826116 | MS.gene26960:CDS | 30.0% |
| AAAGAAGGAGAAAAATGAGG+AGG | - | chr1.3:2825845-2825864 | MS.gene26960:CDS | 35.0% | |
| AACCAAGCAAAGAAACTTCA+AGG | - | chr1.3:2825138-2825157 | MS.gene26960:CDS | 35.0% | |
| AAGAGAGGCTATTTGCTTTA+TGG | - | chr1.3:2825606-2825625 | MS.gene26960:CDS | 35.0% | |
| AAGCTTTACACAAATTGCAC+AGG | - | chr1.3:2825417-2825436 | MS.gene26960:CDS | 35.0% | |
| AATCACGAGTAAATCTGTTG+TGG | - | chr1.3:2825752-2825771 | MS.gene26960:CDS | 35.0% | |
| ATGCAAAAATTGGTAAGCCT+TGG | - | chr1.3:2825583-2825602 | MS.gene26960:CDS | 35.0% | |
| CTTGTTCCAAAACATGGTTA+AGG | + | chr1.3:2825365-2825384 | None:intergenic | 35.0% | |
| GCAATTGTCTCAAAAGAAGA+TGG | + | chr1.3:2825495-2825514 | None:intergenic | 35.0% | |
| TAAGAAAGAAGCAGAGATGA+AGG | - | chr1.3:2826313-2826332 | MS.gene26960:CDS | 35.0% | |
| TACACAAATTGCACAGGTAA+TGG | - | chr1.3:2825423-2825442 | MS.gene26960:CDS | 35.0% | |
| TCATTGGTATTCTCTTCCTT+AGG | + | chr1.3:2826410-2826429 | None:intergenic | 35.0% | |
| TTGAAGTTTCTTTGCTTGGT+TGG | + | chr1.3:2825139-2825158 | None:intergenic | 35.0% | |
| ! | CAGGTAATGGTGAATATGAA+AGG | - | chr1.3:2825436-2825455 | MS.gene26960:CDS | 35.0% |
| ! | CTTTTGTTACCCTTTTGTTG+AGG | - | chr1.3:2825029-2825048 | MS.gene26960:CDS | 35.0% |
| ! | GGTAATGGTGAATATGAAAG+GGG | - | chr1.3:2825438-2825457 | MS.gene26960:CDS | 35.0% |
| ! | TTGAAGGGGTTAAGTTTGTT+TGG | - | chr1.3:2825223-2825242 | MS.gene26960:CDS | 35.0% |
| !! | TAGAATCTTTTGTCTGAAGC+TGG | + | chr1.3:2825294-2825313 | None:intergenic | 35.0% |
| !! | TTTGAACTTTGGGGTCTAAT+AGG | - | chr1.3:2824904-2824923 | MS.gene26960:CDS | 35.0% |
| !!! | CTTAACCATGTTTTGGAACA+AGG | - | chr1.3:2825363-2825382 | MS.gene26960:CDS | 35.0% |
| AATATGAAAGGGGAGGTAAG+TGG | - | chr1.3:2825448-2825467 | MS.gene26960:CDS | 40.0% | |
| AATGGTGAATATGAAAGGGG+AGG | - | chr1.3:2825441-2825460 | MS.gene26960:CDS | 40.0% | |
| ACATCTTCATCAGCTACCTT+AGG | + | chr1.3:2826227-2826246 | None:intergenic | 40.0% | |
| AGCAAAGAACAAAGCAAGTC+AGG | - | chr1.3:2825917-2825936 | MS.gene26960:CDS | 40.0% | |
| ATCCTTTGTTCGAGATGGTT+CGG | - | chr1.3:2826147-2826166 | MS.gene26960:CDS | 40.0% | |
| ATCTCATCCTTTGTTCGAGA+TGG | - | chr1.3:2826142-2826161 | MS.gene26960:CDS | 40.0% | |
| ATGAAGGCTGAGAAAGAAGA+AGG | - | chr1.3:2826329-2826348 | MS.gene26960:CDS | 40.0% | |
| ATGAGTGCATGAACTTGATG+AGG | + | chr1.3:2824982-2825001 | None:intergenic | 40.0% | |
| CAAGTCAGGTAACACTTTCT+GGG | - | chr1.3:2825931-2825950 | MS.gene26960:CDS | 40.0% | |
| CACCTTGAAGTTTCTTTGCT+TGG | + | chr1.3:2825143-2825162 | None:intergenic | 40.0% | |
| CCTTACCTTGTTCCAAAACA+TGG | + | chr1.3:2825371-2825390 | None:intergenic | 40.0% | |
| GATTGTTCGCTTGATCTTAC+CGG | - | chr1.3:2825789-2825808 | MS.gene26960:CDS | 40.0% | |
| GGAGAATGACAGAGAAAAGA+AGG | - | chr1.3:2825830-2825849 | MS.gene26960:CDS | 40.0% | |
| TATTTGCTTTATGGTCCTCC+TGG | - | chr1.3:2825615-2825634 | MS.gene26960:CDS | 40.0% | |
| TGAAGAGGATTCATCAGAGA+AGG | - | chr1.3:2826358-2826377 | MS.gene26960:CDS | 40.0% | |
| TGAAGGCTGAGAAAGAAGAA+GGG | - | chr1.3:2826330-2826349 | MS.gene26960:CDS | 40.0% | |
| TGCTGAAAACTTGATGCCTA+AGG | - | chr1.3:2826208-2826227 | MS.gene26960:CDS | 40.0% | |
| TTGGAACAAGGTAAGGAGAT+TGG | - | chr1.3:2825375-2825394 | MS.gene26960:CDS | 40.0% | |
| ! | AAGTTTTCAGCAACATCAGC+TGG | + | chr1.3:2826200-2826219 | None:intergenic | 40.0% |
| ! | ACTTAGAACCTCTAAGGAAG+AGG | - | chr1.3:2826277-2826296 | MS.gene26960:CDS | 40.0% |
| ! | AGTGATGATTTTCCTGTTCC+AGG | + | chr1.3:2825636-2825655 | None:intergenic | 40.0% |
| ! | ATGTTGAGGCATCATTAGAG+AGG | - | chr1.3:2826243-2826262 | MS.gene26960:CDS | 40.0% |
| ! | CATGTTAGTCTCTTCCAACA+AGG | + | chr1.3:2826174-2826193 | None:intergenic | 40.0% |
| !! | AGGAGATTGGTTTGAGTAAG+AGG | - | chr1.3:2825388-2825407 | MS.gene26960:CDS | 40.0% |
| !!! | CCATGTTTTGGAACAAGGTA+AGG | - | chr1.3:2825368-2825387 | MS.gene26960:CDS | 40.0% |
| AGAGGATTCATCAGAGAAGG+AGG | - | chr1.3:2826361-2826380 | MS.gene26960:CDS | 45.0% | |
| AGCAAATAGCCTCTCTTCCA+AGG | + | chr1.3:2825603-2825622 | None:intergenic | 45.0% | |
| ATTGGTAAGCCTTGGAAGAG+AGG | - | chr1.3:2825591-2825610 | MS.gene26960:CDS | 45.0% | |
| CCAAGCACTTAGAACCTCTA+AGG | - | chr1.3:2826271-2826290 | MS.gene26960:CDS | 45.0% | |
| GACTGCGATGAAATGTGAGA+AGG | + | chr1.3:2825317-2825336 | None:intergenic | 45.0% | |
| GCAAGTCAGGTAACACTTTC+TGG | - | chr1.3:2825930-2825949 | MS.gene26960:CDS | 45.0% | |
| GGTAGCTGATGAAGATGTTG+AGG | - | chr1.3:2826229-2826248 | MS.gene26960:CDS | 45.0% | |
| TGCAGAAGACAAGAAGCCTA+AGG | - | chr1.3:2826391-2826410 | MS.gene26960:CDS | 45.0% | |
| ! | GATCAAGCTTTGATTCGTCG+TGG | - | chr1.3:2826038-2826057 | MS.gene26960:CDS | 45.0% |
| ! | GATGATTTTCCTGTTCCAGG+AGG | + | chr1.3:2825633-2825652 | None:intergenic | 45.0% |
| ! | TTTGATTCGTCGTGGACGAA+TGG | - | chr1.3:2826046-2826065 | MS.gene26960:CDS | 45.0% |
| !! | CCTTAGAGGTTCTAAGTGCT+TGG | + | chr1.3:2826274-2826293 | None:intergenic | 45.0% |
| AGAAGAAGGGACAGGTGAAG+AGG | - | chr1.3:2826343-2826362 | MS.gene26960:CDS | 50.0% | |
| CATCTCAGCCTCTTCCTTAG+AGG | + | chr1.3:2826288-2826307 | None:intergenic | 50.0% | |
| CGAATCTGTCTTCCTCTGAC+CGG | + | chr1.3:2825811-2825830 | None:intergenic | 50.0% | |
| CGGTCAGAGGAAGACAGATT+CGG | - | chr1.3:2825809-2825828 | MS.gene26960:CDS | 50.0% | |
| CTTTATGGTCCTCCTGGAAC+AGG | - | chr1.3:2825621-2825640 | MS.gene26960:CDS | 50.0% | |
| GACCGAACCATCTCGAACAA+AGG | + | chr1.3:2826152-2826171 | None:intergenic | 50.0% | |
| GCTGAGAAAGAAGAAGGGAC+AGG | - | chr1.3:2826335-2826354 | MS.gene26960:CDS | 50.0% | |
| ! | GGGATTTGGTCTGCTAGTAC+TGG | - | chr1.3:2825972-2825991 | MS.gene26960:CDS | 50.0% |
| CGCTTGATCTTACCGGTCAG+AGG | - | chr1.3:2825796-2825815 | MS.gene26960:CDS | 55.0% | |
| ! | AGTTGCTGCTGCTTCTCTGC+AGG | - | chr1.3:2825875-2825894 | MS.gene26960:CDS | 55.0% |
| ! | GTTGCTGCTGCTTCTCTGCA+GGG | - | chr1.3:2825876-2825895 | MS.gene26960:CDS | 55.0% |
| !! | CGAGATGGTTCGGTCCTTGT+TGG | - | chr1.3:2826157-2826176 | MS.gene26960:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 2824901 | 2826439 | 2824901 | ID=MS.gene26960 |
| chr1.3 | mRNA | 2824901 | 2826439 | 2824901 | ID=MS.gene26960.t1;Parent=MS.gene26960 |
| chr1.3 | exon | 2824901 | 2826439 | 2824901 | ID=MS.gene26960.t1.exon1;Parent=MS.gene26960.t1 |
| chr1.3 | CDS | 2824901 | 2826439 | 2824901 | ID=cds.MS.gene26960.t1;Parent=MS.gene26960.t1 |
| Gene Sequence |
| Protein sequence |
