AlfalfaGEDB | MS.gene27552

M. sativa cultivar XinJiangDaYe / MS.gene27552

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005488 GO:0048037 GO:0050662
PFAM: PF01370.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27552.t1	XP_003623714.1	95.4	329	15	0	1	329	1	329	1.60E-181	645.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27552.t1	Q40316	67.2	329	105	1	1	329	1	326	1.5e-130	467.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27552.t1	G7KZB6	95.4	329	15	0	1	329	1	329	1.2e-181	645.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050670	MS.gene27552	0.811292	7.53E-51	-1.69E-46
MS.gene051387	MS.gene27552	0.80145	8.95E-49	-1.69E-46
MS.gene056511	MS.gene27552	0.808077	3.70E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene006771	MS.gene27552	PPI
MS.gene028567	MS.gene27552	PPI
MS.gene031525	MS.gene27552	PPI
MS.gene071401	MS.gene27552	PPI
MS.gene27552	MS.gene071398	PPI
MS.gene40148	MS.gene27552	PPI
MS.gene40154	MS.gene27552	PPI
MS.gene56554	MS.gene27552	PPI
MS.gene96808	MS.gene27552	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27552.t1	MTR_7g074820	95.441	329	15	0	1	329	1	329	0.0	651
MS.gene27552.t1	MTR_7g074850	89.362	329	35	0	1	329	1	329	0.0	616
MS.gene27552.t1	MTR_7g074730	88.146	329	38	1	1	329	1	328	0.0	605
MS.gene27552.t1	MTR_7g074880	75.988	329	76	2	1	329	1	326	0.0	528
MS.gene27552.t1	MTR_7g074710	66.869	329	106	1	1	329	1	326	6.19e-168	470
MS.gene27552.t1	MTR_7g074870	73.264	288	74	2	42	329	4	288	8.38e-156	438
MS.gene27552.t1	MTR_4g023730	49.547	331	163	3	1	329	1	329	6.25e-107	315
MS.gene27552.t1	MTR_1g022440	47.095	327	159	4	8	329	8	325	4.49e-102	303
MS.gene27552.t1	MTR_1g022445	47.095	327	159	4	8	329	8	325	5.37e-98	293
MS.gene27552.t1	MTR_4g092080	42.515	334	181	6	1	327	7	336	4.34e-81	250

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27552.t1	AT2G45400	51.818	330	150	5	6	329	38	364	1.25e-107	318
MS.gene27552.t1	AT5G42800	49.398	332	158	5	1	329	1	325	8.68e-102	304
MS.gene27552.t1	AT2G45400	52.837	282	125	4	6	281	38	317	8.59e-96	287
MS.gene27552.t1	AT2G45400	50.680	294	137	4	41	329	17	307	9.89e-90	271
MS.gene27552.t1	AT4G35420	41.390	331	182	7	1	329	1	321	9.19e-83	254
MS.gene27552.t1	AT1G61720	40.541	333	187	6	4	329	9	337	1.26e-76	238
MS.gene27552.t1	AT2G45400	55.300	217	91	3	6	216	38	254	2.11e-74	230
MS.gene27552.t1	AT1G68540	39.077	325	184	8	10	329	6	321	7.68e-62	200
MS.gene27552.t1	AT1G09480	40.972	288	157	7	1	284	1	279	1.87e-59	194
MS.gene27552.t1	AT2G02400	36.716	335	185	11	4	329	2	318	2.99e-59	193

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Find 63 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAAGAAGAAGAGGTAA+TGG	0.474396	7.2:-30675468	MS.gene27552:CDS
AAAGGAAGAGTTTGTGTGAC+TGG	0.438296	7.2:-30678474	MS.gene27552:CDS
AAAGGTCCAACAACAAAAGT+TGG	0.534220	7.2:+30675305	None:intergenic
AATCAACTGGAGTAGCTGTG+TGG	0.675600	7.2:+30675627	None:intergenic
AATTCCTAATGCTCCATCAA+TGG	0.462695	7.2:+30675565	None:intergenic
AATTGAATGTTGCAAGGAAA+AGG	0.371801	7.2:-30674710	MS.gene27552:CDS
ACACACAGCAGAAGCACTTG+AGG	0.617963	7.2:+30675496	None:intergenic
ACAGCATATGACCAAGCAAA+AGG	0.516501	7.2:+30675401	None:intergenic
ACCACTGTTAGATCTAATCC+AGG	0.585583	7.2:-30678387	MS.gene27552:intron
ACCTGGATTAGATCTAACAG+TGG	0.730654	7.2:+30678386	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAAGAAGAAGAGGTAA+TGG	-	chr7.2:30677717-30677736	MS.gene27552:intron	30.0%
AAAGATTATTAGACCACCTA+AGG	-	chr7.2:30676662-30676681	MS.gene27552:intron	30.0%
AATTGAATGTTGCAAGGAAA+AGG	-	chr7.2:30678475-30678494	MS.gene27552:CDS	30.0%
ACAAAACAGCCTAAGAATAT+AGG	+	chr7.2:30677299-30677318	None:intergenic	30.0%
ACAGCAACATATGATCAATT+AGG	+	chr7.2:30676420-30676439	None:intergenic	30.0%
AGCGAAATTATACAAATTGC+AGG	-	chr7.2:30677409-30677428	MS.gene27552:intron	30.0%
AGCTAAATCTCTTAGAGATT+AGG	-	chr7.2:30676373-30676392	MS.gene27552:intron	30.0%
ATAAGAAAGCTAGTGAACTA+TGG	+	chr7.2:30677933-30677952	None:intergenic	30.0%
ATTATTTCGTAGAACTCACT+TGG	+	chr7.2:30678274-30678293	None:intergenic	30.0%
ATTCTCATCCCTATATTCTT+AGG	-	chr7.2:30677287-30677306	MS.gene27552:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	30674699	30678508	30674699	ID=MS.gene27552
chr7.2	mRNA	30674699	30678508	30674699	ID=MS.gene27552.t1;Parent=MS.gene27552
chr7.2	exon	30678388	30678508	30678388	ID=MS.gene27552.t1.exon1;Parent=MS.gene27552.t1
chr7.2	CDS	30678388	30678508	30678388	ID=cds.MS.gene27552.t1;Parent=MS.gene27552.t1
chr7.2	exon	30675252	30675776	30675252	ID=MS.gene27552.t1.exon2;Parent=MS.gene27552.t1
chr7.2	CDS	30675252	30675776	30675252	ID=cds.MS.gene27552.t1;Parent=MS.gene27552.t1
chr7.2	exon	30674933	30675134	30674933	ID=MS.gene27552.t1.exon3;Parent=MS.gene27552.t1
chr7.2	CDS	30674933	30675134	30674933	ID=cds.MS.gene27552.t1;Parent=MS.gene27552.t1
chr7.2	exon	30674699	30674840	30674699	ID=MS.gene27552.t1.exon4;Parent=MS.gene27552.t1
chr7.2	CDS	30674699	30674840	30674699	ID=cds.MS.gene27552.t1;Parent=MS.gene27552.t1

Gene Sequence

>MS.gene27552

ATGGAAAAAGGGAAAGGAAGAGTTTGTGTGACTGGAGGTACAGGGTTTATTGGTTCATGGATTATCAAGAGACTCCTTGAAGATGGTTATGCTGTTAATACCACTGTTAGATCTAATCCAGGTAACCCCTTAATTCAAATGTTGTTCTTGAATCTTGATTATATATTATGTAACCAAAAAAAGAATTTAAATTGTATTATTTATTTTTCATATTTTTAAGTATCTAATTTGAATTAAGCTGTACATAATATTGTTAAATTAATTCTTCTTAAAAAAAAAATATATATATTCTACTTCGATTAGAAAAAGTTAATGTACCCGGAGAAAATAGAGATAAAACATATGTCCCTAAAAAACATTTGAACTACTTTTATGAATTAACAAAGAAAACATAAAAATAGAGTCCTTAAAAATAGAATATAATAATATTTAATTTTGTTTTTAGTCCTTCTAAAATTTTGTTTTAGATAAAAATTAATAGTGTAAAAATCTAATATACAATGGTTTGAATCTTGAACATCTATTCTAATCATAACTTTTCGTTGTGGCTCAACTCATCATCTAAATTGTCTAATTTCGACACGATGATGATTAGGGAATGTATATTGTATTTTTTATACAAACTTTTATAATATATTGTTGTGAATTCAAACAAATAAATCACATCAATAAAACTATATGTGTGGAAGTATATTCAATGAACAAAGTAGCCTTAAGCAACAATAACAACAAACAAACTTAGATCTAAGTGTGAAGCAACACTACAAACATATTCAAAGCAACAAAGATAAGAAAGAAGAAAAAGAAAACACACAAATATTGTTTATCCAGTTCGGTCCAATAAGACATACTCTAGGGAGAAAACAACTCTCCGATCCACTATGATAAAGAGTGATACAAGTAGATTACAAGAAATTAACTTAGAAGCTTACAAGTCACCCACACTCACAATGAATCCTTAGAGAAAACACTAAATGAAGTTTTTTGATCATTCCTCTTGAGATCTCACTACCCAAGGTGTTTATAAATATTTCTCAACCCAAGAAAAAACTCTCACCAAAGACACCTAGTCCTAAAGTTCCCTCCTTTGGTTTTCCTATCTCTTGAAGCTCCCTAAAAAAATGAAGAATTGAAGCATTATATAGTACCCAGAATATCCTGAGAAATCGTTCCACGATTGGCACTGATGACATGCTGTCATTACGTAATTTTTAGGACTTATTTTGACTTTTTGATAGAGTTCTAAGCAACATTAAGTGTCCAGGAGCAGTTAAAAGCAACAAATTTGGAAGATCTGGAACCAGCCAATCGTACTGCGATTTTCCAATCGCAATGCGATTTCTTGAAGTCAGAGAGCATGTTTTTGAAGGAGCTGGATCGCAATACGATCCAGCCAATCGTGATACGATTTCAAGAAGGGCTTTGAAGTAAATTAGGGTTTAAAGATGCAATCGTGGTACGATTTCTCTAATCGTGGTATGATTTTGGGCGATCAGAACTTGCCTATTTAAGCTGAAGTCAGTCTCAAACAAAGGGGATTACGATTTTGGAGAGCTAAAGTGCGATTTTAACACCCAGAAGGCTGCAGCGGAGAATATTTCATCATCTCTAACCCAGTTTATGTATGTTTTAATTCTTTCATTGATTATGCTATTTGTAAACTCAATTATGAGTAGCTAAATCTCTTAGAGATTAGGTCAGAGATGAATCTATGTAACCCTAATTGATCATATGTTGCTGTGAAACTCTATTAGTTATTAATGAATTTCTAGTCTTTATTCAATTCAATTGTTCTTAATGCTTTTTATATCCTGATCAAATATAAACATGATTTTGTGAGAACGAATGACTACTGACCATAGCTTTCGACTTAGACCTAATTGAATTGTTCTAATAAACATGGTTAGTCTAGGAATGGATAATCGTTTGTTAGTGATATCAGGTTAACATTCATAAAACCTTCAAAGATTATTAGACCACCTAAGGAATTAGGGGTTGTAGTTTAAGAAGAGGGTCCTAACTCAAGGGATTGATTAGAGCTAATTTGTTAACTATCGTGAAACTAGAAAATCATTAGTAACAATAGGTGCAGTAACCAATTAGGGGTAACATAGAGGATTCGAAGACCTGATCTCATCTCTCTCCATATTTTCTTAATCAAACCTAATTCTCTTAGTTGATTTCATAAAATCAAACCAAATCTACTGCCTAGTGGCAAAACAAAATAAACTTTTATACATCCTATTTTTATAACGCGATTGAATTATTGAAGTTAAAAACAGTCCTCGTGGGATCGATATTTTTACTACTTCGATAGTAATTGGTGCACTTGCCAATCCTCTATCAGGCACCCATCGTGCCACGATTTGTTGTTGGTACACCAATACAGTACTAACTTTATCTGTTAATCGTGCCACAATTTCATGCATAGGTGTCACGATATTTCCAATAGCAAACTCCGTGGAAAAGAATCAAAATTGTGCCACAATTTTTCTAAAACTTCAACATCCAATTTTGAGCTAAATTTCAACATATAAAATCTCGCCCCTGTCTTCAAATTTTTTGGATCCGTTCCTGGTCAAACACATATTCTCATCCCTATATTCTTAGGCTGTTTTGTCAACCTCTTAACTAAAATTCAAATCACGTGCCAATTTTGTTTATTTGTTGTTATTAATATTATTGTTTTCCATGTAACGATAGATATGTAAGCGAAATTATACAAATTGCAGGGCAAAAGAAAGATGTTAGCTTCCTCACAGATCTTCCAAATGCAACCCAAAAGCTAAATATTTTCAATGCTGATCTTAGCAACCCAGAAAGTTTCAATGCAGCAATTGAAGGGTGCATTGGAGTATTCCACACAGCTACTCCAGTTGATTTTGAACTGAAAGAACCAGAGGAAATTGTGATAAAAAGAACCATTGATGGAGCATTAGGAATTCTAAAAGCATGCAAAAATTCAAAGACAGTGAAAAGAGTTATTTACACCTCAAGTGCTTCTGCTGTGTGTATGCAAAACAAAGAAGAAGAGGTAATGGATGAAAGTTATTGGAGTGATGTGAATATTCTTAGAAATTTGAAGCCTTTTGCTTGGTCATATGCTGTTTCTAAGACACTAGCTGAGAAAGCTGTTCTAGAATTTGGAGAACAACATGGTTTGGATATCGTGACTCTAATACCAACTTTTGTTGTTGGACCTTTCATTTGTCCTAAGCTACCTGGATCTGTCCATAGTTCACTAGCTTTCTTATTTGGTAAGTGAAGTTTCTTCTTACCATGTTTCTCATTGGTTCTTGGACTATTGTGAAAGTATTTTCTTGATTATTTTATTTCTTAAATTGATAAAAATTAACAAAGGAATTTTCATGCAGGTGATATTGACAAGAATCCATTAGTGCTTGTATCTCGTACCGGTATGGTGCATGTTGATGATGTGGCTAGAGCACATATATTCTTACTTGAACATCCTAATCCAAAAGGAAGATATAATTGCTCACCATTCATTGCAACTATTGAAGAAATAGTTGACCTTGTTTCTTCCAAGTATCCTGAACTTCAAATGCCAACATCCAAGTGAGTTCTACGAAATAATTAGTTACATTAATTTGTGTGAGCTTTCTTTTGTTTTTACTAATAATATTATTCAATGTATTTTCATCTTCTAGAGAGCTTATGGGAGTTAAAGGTCCTAAGTTTCCACATTTAACCTCAAAGAAACTCGTGGATGATGGATTCATGTTCAAGTATAGCCTTGAGGAAATGTTTGAGGACGCAATTGAATGTTGCAAGGAAAAGGGTTATCTTTGA

Protein sequence

>MS.gene27552.t1

MEKGKGRVCVTGGTGFIGSWIIKRLLEDGYAVNTTVRSNPGQKKDVSFLTDLPNATQKLNIFNADLSNPESFNAAIEGCIGVFHTATPVDFELKEPEEIVIKRTIDGALGILKACKNSKTVKRVIYTSSASAVCMQNKEEEVMDESYWSDVNILRNLKPFAWSYAVSKTLAEKAVLEFGEQHGLDIVTLIPTFVVGPFICPKLPGSVHSSLAFLFGDIDKNPLVLVSRTGMVHVDDVARAHIFLLEHPNPKGRYNCSPFIATIEEIVDLVSSKYPELQMPTSKELMGVKGPKFPHLTSKKLVDDGFMFKYSLEEMFEDAIECCKEKGYL