Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29219.t1 | XP_024640266.1 | 99.2 | 362 | 3 | 0 | 38 | 399 | 66 | 427 | 7.50E-205 | 723 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29219.t1 | Q08A71 | 73.2 | 399 | 101 | 4 | 6 | 399 | 38 | 435 | 1.5e-164 | 580.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29219.t1 | A0A2Z6MTP5 | 93.9 | 360 | 16 | 1 | 40 | 399 | 74 | 427 | 6.2e-193 | 682.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29219 | MS.gene067338 | PPI |
| MS.gene66341 | MS.gene29219 | PPI |
| MS.gene29219 | MS.gene75060 | PPI |
| MS.gene75062 | MS.gene29219 | PPI |
| MS.gene038866 | MS.gene29219 | PPI |
| MS.gene29219 | MS.gene064036 | PPI |
| MS.gene29219 | MS.gene030293 | PPI |
| MS.gene68888 | MS.gene29219 | PPI |
| MS.gene29219 | MS.gene66341 | PPI |
| MS.gene29219 | MS.gene64396 | PPI |
| MS.gene83327 | MS.gene29219 | PPI |
| MS.gene064036 | MS.gene29219 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29219.t1 | MTR_1g113940 | 52.793 | 358 | 124 | 6 | 50 | 394 | 11 | 336 | 1.32e-120 | 353 |
| MS.gene29219.t1 | MTR_6g091770 | 38.636 | 352 | 177 | 6 | 40 | 390 | 57 | 370 | 2.39e-79 | 249 |
| MS.gene29219.t1 | MTR_2g054880 | 37.709 | 358 | 171 | 8 | 51 | 385 | 26 | 354 | 1.22e-63 | 208 |
| MS.gene29219.t1 | MTR_3g013960 | 38.393 | 336 | 149 | 8 | 50 | 357 | 255 | 560 | 6.02e-60 | 205 |
| MS.gene29219.t1 | MTR_4g119900 | 36.364 | 341 | 174 | 8 | 51 | 383 | 142 | 447 | 8.68e-57 | 195 |
| MS.gene29219.t1 | MTR_4g119900 | 36.364 | 341 | 174 | 8 | 51 | 383 | 142 | 447 | 9.34e-57 | 194 |
| MS.gene29219.t1 | MTR_2g436780 | 36.086 | 327 | 168 | 7 | 51 | 369 | 144 | 437 | 3.84e-55 | 190 |
| MS.gene29219.t1 | MTR_2g436780 | 36.086 | 327 | 168 | 7 | 51 | 369 | 144 | 437 | 5.05e-55 | 190 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29219.t1 | AT3G20020 | 72.772 | 404 | 94 | 5 | 6 | 399 | 38 | 435 | 0.0 | 611 |
| MS.gene29219.t1 | AT3G20020 | 72.634 | 391 | 91 | 5 | 6 | 386 | 38 | 422 | 0.0 | 592 |
| MS.gene29219.t1 | AT3G20020 | 68.069 | 404 | 91 | 6 | 6 | 399 | 38 | 413 | 0.0 | 562 |
| MS.gene29219.t1 | AT2G19670 | 39.466 | 337 | 168 | 5 | 51 | 386 | 48 | 349 | 9.14e-83 | 258 |
| MS.gene29219.t1 | AT4G29510 | 37.892 | 351 | 179 | 6 | 37 | 386 | 61 | 373 | 3.52e-81 | 254 |
| MS.gene29219.t1 | AT3G12270 | 38.251 | 366 | 179 | 11 | 15 | 365 | 211 | 544 | 1.31e-67 | 225 |
| MS.gene29219.t1 | AT1G04870 | 37.079 | 356 | 172 | 10 | 51 | 383 | 35 | 361 | 2.15e-66 | 216 |
| MS.gene29219.t1 | AT3G06930 | 35.692 | 325 | 172 | 6 | 51 | 369 | 148 | 441 | 4.04e-56 | 193 |
| MS.gene29219.t1 | AT3G06930 | 35.692 | 325 | 172 | 6 | 51 | 369 | 148 | 441 | 4.16e-56 | 193 |
| MS.gene29219.t1 | AT5G49020 | 33.923 | 339 | 187 | 6 | 51 | 383 | 149 | 456 | 6.67e-55 | 189 |
| MS.gene29219.t1 | AT5G49020 | 33.923 | 339 | 187 | 6 | 51 | 383 | 151 | 458 | 6.71e-55 | 189 |
| MS.gene29219.t1 | AT1G04870 | 34.843 | 287 | 135 | 10 | 120 | 383 | 1 | 258 | 3.08e-41 | 147 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGCTTCTAATTCCATTGTT+AGG | 0.210087 | 1.1:+23525223 | MS.gene29219:CDS |
| GTGTGCGGACTGAAACTTAT+AGG | 0.254061 | 1.1:+23526611 | MS.gene29219:CDS |
| CGGTGTTGATGAATGATTAT+TGG | 0.306970 | 1.1:-23537363 | None:intergenic |
| TGATCTTGTTCCAGCTCAAT+AGG | 0.331644 | 1.1:-23537702 | None:intergenic |
| ACCGGCCTGAGCACAAAATA+TGG | 0.332954 | 1.1:-23527277 | None:intergenic |
| TACAGTTTCTAACTCATTGA+TGG | 0.342313 | 1.1:-23536901 | None:intergenic |
| CGCTTCTAATTCCATTGTTA+GGG | 0.346716 | 1.1:+23525224 | MS.gene29219:CDS |
| ATTTGCTTTGACAACTTCAT+TGG | 0.373402 | 1.1:-23530969 | None:intergenic |
| TCCTTGCACTGATTACGATA+TGG | 0.382128 | 1.1:+23525299 | MS.gene29219:CDS |
| ACAGTGACAGCGTTGACTTT+TGG | 0.397498 | 1.1:+23533926 | MS.gene29219:CDS |
| ACCTGATGATTCTCAATAAC+CGG | 0.408242 | 1.1:-23537383 | None:intergenic |
| CAATGTGTTGGAGGGTCCTC+AGG | 0.428562 | 1.1:-23537467 | None:intergenic |
| GGTCCTCAGGAGCAGTAGAT+AGG | 0.435974 | 1.1:-23537454 | None:intergenic |
| ACCTGCTGCCAATGTGTTGG+AGG | 0.436915 | 1.1:-23537476 | None:intergenic |
| CTGCTGTTGCGGCGGTTGTT+CGG | 0.440441 | 1.1:-23525256 | None:intergenic |
| TATCTATCGGTATGTGTGAC+GGG | 0.443340 | 1.1:-23533904 | None:intergenic |
| AGGTTTGAGCCAGCGATCTC+TGG | 0.443984 | 1.1:-23533739 | None:intergenic |
| ATTATTGGTTGGTGCTATCA+TGG | 0.447582 | 1.1:-23537348 | None:intergenic |
| GTTGATGTTGGATGCGGTAC+AGG | 0.448555 | 1.1:+23527246 | MS.gene29219:CDS |
| TGCTCAGGCCGGTGCAAAGC+GGG | 0.449385 | 1.1:+23527287 | MS.gene29219:CDS |
| TGTGCGGACTGAAACTTATA+GGG | 0.452856 | 1.1:+23526612 | MS.gene29219:CDS |
| GGGCTATATGCTTCTATATG+AGG | 0.459298 | 1.1:+23532269 | MS.gene29219:CDS |
| TGTTATAATTTCAGAATGGA+TGG | 0.463270 | 1.1:+23532248 | MS.gene29219:CDS |
| AGAAATACCGTTGCACTTGA+AGG | 0.467588 | 1.1:-23533777 | None:intergenic |
| CCTGCTGTGGAGACGATAAC+TGG | 0.474816 | 1.1:+23534993 | MS.gene29219:CDS |
| CTTCAGGTCGTAGTTGATGT+TGG | 0.480894 | 1.1:+23527234 | MS.gene29219:intron |
| GAACAAGATCAAGTTATTGA+CGG | 0.493723 | 1.1:+23537714 | MS.gene29219:CDS |
| CCCTCCAACACATTGGCAGC+AGG | 0.494539 | 1.1:+23537475 | MS.gene29219:CDS |
| CCAGTTATCGTCTCCACAGC+AGG | 0.495006 | 1.1:-23534993 | None:intergenic |
| TGCACCTGCTGCCAATGTGT+TGG | 0.509483 | 1.1:-23537479 | None:intergenic |
| AGAGATCGCTGGCTCAAACC+TGG | 0.509562 | 1.1:+23533741 | MS.gene29219:CDS |
| GTATCTATCGGTATGTGTGA+CGG | 0.511759 | 1.1:-23533905 | None:intergenic |
| CTTCTATGATCCTATTGAGC+TGG | 0.512472 | 1.1:+23537692 | MS.gene29219:CDS |
| TATTCACCAGGAAATGATCA+AGG | 0.514595 | 1.1:+23525350 | MS.gene29219:CDS |
| TTTCATTCCTATGCTCATCT+CGG | 0.515727 | 1.1:+23525327 | MS.gene29219:CDS |
| AGAGCATACCCGCTTTGCAC+CGG | 0.516717 | 1.1:-23527295 | None:intergenic |
| TACCTGCAGTGCAATGTCAC+TGG | 0.520846 | 1.1:-23528435 | None:intergenic |
| ACCGGTTATTGAGAATCATC+AGG | 0.527282 | 1.1:+23537382 | MS.gene29219:CDS |
| TGCCAGTGACATTGCACTGC+AGG | 0.527330 | 1.1:+23528433 | MS.gene29219:CDS |
| AGCGAATCCTAGTGAAGCAC+TGG | 0.534199 | 1.1:+23537430 | MS.gene29219:CDS |
| TTATTACTGCCAGAGATCGC+TGG | 0.544782 | 1.1:+23533730 | MS.gene29219:CDS |
| ATTGAGAATCATCAGGTGAA+TGG | 0.545325 | 1.1:+23537389 | MS.gene29219:CDS |
| TTCTAACTCATTGATGGTGA+CGG | 0.545702 | 1.1:-23536895 | None:intergenic |
| TATTCTTCCTTCAAGTGCAA+CGG | 0.546213 | 1.1:+23533770 | MS.gene29219:CDS |
| TGGATGTTATAATTTCAGAA+TGG | 0.561126 | 1.1:+23532244 | MS.gene29219:CDS |
| TGTATTGCATGGACGAGTTG+AGG | 0.564415 | 1.1:+23531012 | MS.gene29219:CDS |
| GTTATAATTTCAGAATGGAT+GGG | 0.566137 | 1.1:+23532249 | MS.gene29219:CDS |
| TCAGTGCAAGGAGATCGATT+AGG | 0.566907 | 1.1:-23525288 | None:intergenic |
| GTCGTAGTTGATGTTGGATG+CGG | 0.571602 | 1.1:+23527240 | MS.gene29219:CDS |
| CCTGCTGCCAATGTGTTGGA+GGG | 0.574057 | 1.1:-23537475 | None:intergenic |
| TGCTCATCTCGGTATTCACC+AGG | 0.574181 | 1.1:+23525338 | MS.gene29219:CDS |
| TAGAAGCGGAAGCGCGTGAA+CGG | 0.577232 | 1.1:-23525209 | None:intergenic |
| TGAACGGCGAGTGCCACGAC+GGG | 0.577599 | 1.1:-23525193 | None:intergenic |
| AACGCTGTCACTGTATCTAT+CGG | 0.578612 | 1.1:-23533917 | None:intergenic |
| GGTAGTTGTTGCTGCTGTTG+CGG | 0.581774 | 1.1:-23525267 | None:intergenic |
| ATCTATCGGTATGTGTGACG+GGG | 0.584626 | 1.1:-23533903 | None:intergenic |
| GGCGAGTGCCACGACGGGCA+CGG | 0.587048 | 1.1:-23525188 | None:intergenic |
| GGTCCTATCTACTGCTCCTG+AGG | 0.587466 | 1.1:+23537451 | MS.gene29219:CDS |
| GTTGATGAATGATTATTGGT+TGG | 0.587903 | 1.1:-23537359 | None:intergenic |
| CAAACTCACCGTGCCCGTCG+TGG | 0.590673 | 1.1:+23525180 | MS.gene29219:CDS |
| GATGTCGTCATTGTATTGCA+TGG | 0.590963 | 1.1:+23531001 | MS.gene29219:CDS |
| CTGAGGACCCTCCAACACAT+TGG | 0.592000 | 1.1:+23537468 | MS.gene29219:CDS |
| GTGCTCAGGCCGGTGCAAAG+CGG | 0.594460 | 1.1:+23527286 | MS.gene29219:CDS |
| TTATTGGTTGGTGCTATCAT+GGG | 0.600178 | 1.1:-23537347 | None:intergenic |
| GTGAATACCGAGATGAGCAT+AGG | 0.600534 | 1.1:-23525334 | None:intergenic |
| GATAGGACCAGTGCTTCACT+AGG | 0.604827 | 1.1:-23537437 | None:intergenic |
| CCTAACAATGGAATTAGAAG+CGG | 0.615088 | 1.1:-23525223 | None:intergenic |
| ATCCGACTCACGTATACTCA+AGG | 0.615155 | 1.1:-23537788 | None:intergenic |
| GGTATCAGAAACCCTAACAA+TGG | 0.615889 | 1.1:-23525235 | None:intergenic |
| TGCATTTGAAGAACCTGCTG+TGG | 0.618799 | 1.1:+23534980 | MS.gene29219:CDS |
| CCTGAGCACAAAATATGGAA+AGG | 0.619755 | 1.1:-23527272 | None:intergenic |
| GCCATATCGTAATCAGTGCA+AGG | 0.625085 | 1.1:-23525300 | None:intergenic |
| CAATAATCATTCATCAACAC+CGG | 0.627958 | 1.1:+23537364 | MS.gene29219:CDS |
| CAAAATGCAAAACCATGCAG+TGG | 0.630371 | 1.1:-23537302 | None:intergenic |
| ATACAATGACGACATCAGAA+AGG | 0.634777 | 1.1:-23530994 | None:intergenic |
| GTTTGGTACAGGATCGTGTG+CGG | 0.635116 | 1.1:+23526596 | MS.gene29219:intron |
| GAAGGAAGAATAAGACCACC+AGG | 0.640805 | 1.1:-23533759 | None:intergenic |
| AGTTGTTGCTGCTGTTGCGG+CGG | 0.643125 | 1.1:-23525264 | None:intergenic |
| GGATGTTGAAATCGATGAAG+AGG | 0.655942 | 1.1:+23532221 | MS.gene29219:intron |
| GTGAACGGCGAGTGCCACGA+CGG | 0.656629 | 1.1:-23525194 | None:intergenic |
| CGACTCACGTATACTCAAGG+TGG | 0.658948 | 1.1:-23537785 | None:intergenic |
| AAGTTCAACTCAATGATGCG+AGG | 0.662822 | 1.1:+23536936 | MS.gene29219:CDS |
| TGTTGAAATCGATGAAGAGG+TGG | 0.668496 | 1.1:+23532224 | MS.gene29219:CDS |
| ACTAGCATACTCACCACGTG+TGG | 0.671522 | 1.1:-23535035 | None:intergenic |
| GATCGCTGGCTCAAACCTGG+TGG | 0.682355 | 1.1:+23533744 | MS.gene29219:CDS |
| TCTATCGGTATGTGTGACGG+GGG | 0.691322 | 1.1:-23533902 | None:intergenic |
| CTTATAGGGAAGCAATCATG+CGG | 0.705648 | 1.1:+23526626 | MS.gene29219:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 23525174 | 23537928 | 23525174 | ID=MS.gene29219 |
| chr1.1 | mRNA | 23525174 | 23537928 | 23525174 | ID=MS.gene29219.t1;Parent=MS.gene29219 |
| chr1.1 | exon | 23525174 | 23525371 | 23525174 | ID=MS.gene29219.t1.exon1;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23525174 | 23525371 | 23525174 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23526607 | 23526672 | 23526607 | ID=MS.gene29219.t1.exon2;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23526607 | 23526672 | 23526607 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23527240 | 23527308 | 23527240 | ID=MS.gene29219.t1.exon3;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23527240 | 23527308 | 23527240 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23528419 | 23528454 | 23528419 | ID=MS.gene29219.t1.exon4;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23528419 | 23528454 | 23528419 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23530968 | 23531033 | 23530968 | ID=MS.gene29219.t1.exon5;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23530968 | 23531033 | 23530968 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23532222 | 23532290 | 23532222 | ID=MS.gene29219.t1.exon6;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23532222 | 23532290 | 23532222 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23533714 | 23533791 | 23533714 | ID=MS.gene29219.t1.exon7;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23533714 | 23533791 | 23533714 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23533892 | 23533970 | 23533892 | ID=MS.gene29219.t1.exon8;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23533892 | 23533970 | 23533892 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23534949 | 23535043 | 23534949 | ID=MS.gene29219.t1.exon9;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23534949 | 23535043 | 23534949 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23536873 | 23536957 | 23536873 | ID=MS.gene29219.t1.exon10;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23536873 | 23536957 | 23536873 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23537300 | 23537496 | 23537300 | ID=MS.gene29219.t1.exon11;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23537300 | 23537496 | 23537300 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23537681 | 23537799 | 23537681 | ID=MS.gene29219.t1.exon12;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23537681 | 23537799 | 23537681 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| chr1.1 | exon | 23537886 | 23537928 | 23537886 | ID=MS.gene29219.t1.exon13;Parent=MS.gene29219.t1 |
| chr1.1 | CDS | 23537886 | 23537928 | 23537886 | ID=cds.MS.gene29219.t1;Parent=MS.gene29219.t1 |
| Gene Sequence |
| Protein sequence |
