AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68888


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68888.t1 MTR_6g091770 100.000 306 0 0 1 306 78 383 0.0 640
MS.gene68888.t1 MTR_1g113940 35.048 311 181 6 1 293 25 332 9.41e-52 174
MS.gene68888.t1 MTR_3g013960 36.789 299 151 9 1 264 269 564 3.26e-51 178
MS.gene68888.t1 MTR_4g119900 36.299 281 166 7 1 270 155 433 2.17e-50 174
MS.gene68888.t1 MTR_2g054880 32.836 335 192 10 1 303 39 372 3.08e-50 171
MS.gene68888.t1 MTR_4g119900 36.299 281 166 7 1 270 155 433 3.29e-50 174
MS.gene68888.t1 MTR_2g436780 35.231 281 169 7 1 270 157 435 8.21e-48 167
MS.gene68888.t1 MTR_2g436780 35.231 281 169 7 1 270 157 435 1.07e-47 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68888.t1 AT4G29510 82.680 306 53 0 1 306 85 390 0.0 549
MS.gene68888.t1 AT2G19670 79.085 306 64 0 1 306 61 366 0.0 527
MS.gene68888.t1 AT3G20020 39.157 332 162 4 1 293 96 426 2.49e-74 234
MS.gene68888.t1 AT3G20020 39.210 329 160 4 1 290 96 423 1.24e-73 233
MS.gene68888.t1 AT3G20020 36.446 332 149 4 1 293 96 404 1.26e-62 204
MS.gene68888.t1 AT3G12270 37.801 291 156 8 1 269 258 545 1.62e-54 187
MS.gene68888.t1 AT1G04870 33.019 318 177 8 1 286 48 361 2.10e-51 174
MS.gene68888.t1 AT3G06930 33.663 303 188 7 1 292 161 461 3.18e-49 171
MS.gene68888.t1 AT3G06930 33.663 303 188 7 1 292 161 461 3.30e-49 171
MS.gene68888.t1 AT5G49020 33.876 307 190 7 1 296 162 466 5.16e-49 171
MS.gene68888.t1 AT5G49020 33.876 307 190 7 1 296 164 468 5.91e-49 171
MS.gene68888.t1 AT1G04870 29.008 262 150 8 57 286 1 258 2.49e-29 113

Find 0 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCTTAAAAAAAGAAAATT+GGG + 42522:2323-2342 MS.gene68888:intron 10.0%
!! TATTCTTAAAAAAAGAAAAT+TGG + 42522:2322-2341 MS.gene68888:intron 10.0%
!! AAATGATATTATAACAAAGA+CGG - 42522:2618-2637 None:intergenic 15.0%
!! TTCTTAAAAAAAGAAAATTG+GGG + 42522:2324-2343 MS.gene68888:intron 15.0%
!!! ATTCATTTTTATCATTTTAG+TGG + 42522:2653-2672 MS.gene68888:intron 15.0%
!!! TTGTTATAATATCATTTTAG+TGG + 42522:2621-2640 MS.gene68888:intron 15.0%
!!! TTTCTTTTTTTTTTTTGTGT+AGG + 42522:1413-1432 MS.gene68888:intron 15.0%
!! AAAACGTTATTTATCAGAAT+AGG + 42522:781-800 MS.gene68888:CDS 20.0%
!! ATTTGTGTTTAAACAAAACT+AGG - 42522:1694-1713 None:intergenic 20.0%
!! GAAATGATTATATCAACTTT+GGG - 42522:1224-1243 None:intergenic 20.0%
!! TTTGTAATCTTTATCTTCAA+TGG - 42522:1487-1506 None:intergenic 20.0%
!!! AATTTTAAAACTGTTCCAAA+TGG + 42522:2384-2403 MS.gene68888:intron 20.0%
!!! ATTTTAAAACTGTTCCAAAT+GGG + 42522:2385-2404 MS.gene68888:intron 20.0%
!!! CATGTATTTTTTCTGAAAAT+CGG + 42522:2539-2558 MS.gene68888:intron 20.0%
!!! GATGAAATTTTCTTTTCATT+TGG + 42522:1973-1992 MS.gene68888:intron 20.0%
! AAACAAAACTAGGAATTTCA+AGG - 42522:1684-1703 None:intergenic 25.0%
! AACTGTAGAAAACAACATTA+TGG - 42522:1156-1175 None:intergenic 25.0%
! AAGGAAATAGTTGAAACTAA+TGG + 42522:1022-1041 MS.gene68888:CDS 25.0%
! AATAAAGTAGTTCTTGATGT+CGG + 42522:816-835 MS.gene68888:CDS 25.0%
! GAAACTAATGGTTATTCTAA+AGG + 42522:1034-1053 MS.gene68888:CDS 25.0%
! GGAAATGATTATATCAACTT+TGG - 42522:1225-1244 None:intergenic 25.0%
! TAAAAAAAGAAAATTGGGGA+GGG + 42522:2328-2347 MS.gene68888:intron 25.0%
! TGTGTACATATATATTTCCA+GGG + 42522:2694-2713 MS.gene68888:intron 25.0%
! TTAAAAAAAGAAAATTGGGG+AGG + 42522:2327-2346 MS.gene68888:intron 25.0%
! TTCCAAACTGATATAGAAAA+TGG - 42522:1764-1783 None:intergenic 25.0%
! TTGATATAATCATTTCCGAA+TGG + 42522:1228-1247 MS.gene68888:CDS 25.0%
! TTGTGTACATATATATTTCC+AGG + 42522:2693-2712 MS.gene68888:intron 25.0%
! TTTCAGAGACAAATAAGTAT+TGG - 42522:1587-1606 None:intergenic 25.0%
!! ACAAAGTGTCATAAATTGAT+GGG + 42522:2177-2196 MS.gene68888:CDS 25.0%
!! TACAAAGTGTCATAAATTGA+TGG + 42522:2176-2195 MS.gene68888:CDS 25.0%
!! TTCCATTTTCTATATCAGTT+TGG + 42522:1759-1778 MS.gene68888:intron 25.0%
!!! AATAACGTTTTGATATGTCT+TGG - 42522:772-791 None:intergenic 25.0%
!!! ACAGTTATAACAGTTTTGAA+AGG + 42522:1170-1189 MS.gene68888:intron 25.0%
!!! CAGTTATAACAGTTTTGAAA+GGG + 42522:1171-1190 MS.gene68888:intron 25.0%
!!! CTTTTTTTTTTTTGTGTAGG+TGG + 42522:1416-1435 MS.gene68888:intron 25.0%
AAAAAAAGAAAATTGGGGAG+GGG + 42522:2329-2348 MS.gene68888:intron 30.0%
ACTACTTATCAACAAGAGTA+AGG - 42522:1334-1353 None:intergenic 30.0%
ACTCAATCATATTTAGCAAG+AGG - 42522:1538-1557 None:intergenic 30.0%
ATAATCATTTCCGAATGGAT+GGG + 42522:1233-1252 MS.gene68888:CDS 30.0%
ATATTGCATGTACTAGAATC+TGG + 42522:1361-1380 MS.gene68888:intron 30.0%
ATGCTCAAGTATTCATTGAA+TGG + 42522:2852-2871 MS.gene68888:CDS 30.0%
CAAATGATATTTGTGTCATC+AGG + 42522:1709-1728 MS.gene68888:intron 30.0%
CAATTTCTCAATTCCGTATT+CGG - 42522:1638-1657 None:intergenic 30.0%
CCACAAAAACTAAAGTAGAA+AGG - 42522:2221-2240 None:intergenic 30.0%
CTTGTATTTCATTCCGAATA+CGG + 42522:1622-1641 MS.gene68888:intron 30.0%
GACATAATTCAAAACCCATT+TGG - 42522:2402-2421 None:intergenic 30.0%
GGGATTATTATCAATTAGTG+TGG + 42522:2351-2370 MS.gene68888:intron 30.0%
GTAATCTTTATCTTCAATGG+CGG - 42522:1484-1503 None:intergenic 30.0%
TATAATCATTTCCGAATGGA+TGG + 42522:1232-1251 MS.gene68888:CDS 30.0%
TGCGTGTGCAAAATTTAAAA+TGG - 42522:971-990 None:intergenic 30.0%
TGTATGAAACATACAATGCA+AGG - 42522:1939-1958 None:intergenic 30.0%
TTAACTGAACTGAAAAGAAC+TGG + 42522:2493-2512 MS.gene68888:intron 30.0%
! ATTATATCAACTTTGGGAAC+AGG - 42522:1218-1237 None:intergenic 30.0%
! ATTTTAAATTTTGCACACGC+AGG + 42522:970-989 MS.gene68888:intron 30.0%
! TCAGTTTGGAACAATGTATA+TGG + 42522:1773-1792 MS.gene68888:intron 30.0%
!! GAGACAAATAAGTATTGGTT+TGG - 42522:1582-1601 None:intergenic 30.0%
!! GTTTTGAATTATGTCACTTG+AGG + 42522:2407-2426 MS.gene68888:intron 30.0%
!!! CCTTTCTACTTTAGTTTTTG+TGG + 42522:2218-2237 MS.gene68888:intron 30.0%
AAAAAAGAAAATTGGGGAGG+GGG + 42522:2330-2349 MS.gene68888:intron 35.0%
AGATAGAGTGATGCAATATC+AGG - 42522:1459-1478 None:intergenic 35.0%
CAAGAAATATCCCATCCATT+CGG - 42522:1246-1265 None:intergenic 35.0%
CCCAAATAAAAAGAATCCTC+GGG + 42522:2818-2837 MS.gene68888:CDS 35.0%
CCCCAAATAAAAAGAATCCT+CGG + 42522:2817-2836 MS.gene68888:CDS 35.0%
GAAACAAACAGTCCTATATC+TGG + 42522:2740-2759 MS.gene68888:CDS 35.0%
GACATCTCAAAAATGTCTTC+TGG + 42522:2066-2085 MS.gene68888:CDS 35.0%
GATAGAGTGATGCAATATCA+GGG - 42522:1458-1477 None:intergenic 35.0%
TAGTGATAAAATTCCAGTCC+CGG - 42522:844-863 None:intergenic 35.0%
TCAAGACATCTTCCAGATAT+AGG - 42522:2755-2774 None:intergenic 35.0%
TCAAGTATTCATTGAATGGC+AGG + 42522:2856-2875 MS.gene68888:CDS 35.0%
TTCAGTATATTACCTTCTGC+CGG + 42522:1096-1115 MS.gene68888:intron 35.0%
! TCCCGAGGATTCTTTTTATT+TGG - 42522:2822-2841 None:intergenic 35.0%
!! CATAAAAGCATCATCAGAAC+TGG - 42522:1907-1926 None:intergenic 35.0%
!! CCCGAGGATTCTTTTTATTT+GGG - 42522:2821-2840 None:intergenic 35.0%
!! CCGAGGATTCTTTTTATTTG+GGG - 42522:2820-2839 None:intergenic 35.0%
!!! AGACATTTTTGAGATGTCCA+TGG - 42522:2064-2083 None:intergenic 35.0%
!!! TTTTTTGTGTAGGTGGATGA+TGG + 42522:1423-1442 MS.gene68888:intron 35.0%
AAAAAGAAAATTGGGGAGGG+GGG + 42522:2331-2350 MS.gene68888:intron 40.0%
AACAAAGACGGAACCACAAA+TGG - 42522:2606-2625 None:intergenic 40.0%
ACAAAGACGGAACCACAAAT+GGG - 42522:2605-2624 None:intergenic 40.0%
AGATGTCTTGACCATTTGTG+AGG + 42522:2764-2783 MS.gene68888:CDS 40.0%
AGCATTATATCAACATCCCG+AGG - 42522:2837-2856 None:intergenic 40.0%
ATGTCTTGACCATTTGTGAG+GGG + 42522:2766-2785 MS.gene68888:CDS 40.0%
GATGTCTTGACCATTTGTGA+GGG + 42522:2765-2784 MS.gene68888:CDS 40.0%
GTTCATCTTTGTTCAGTCCA+TGG + 42522:2044-2063 MS.gene68888:intron 40.0%
TTCAACAGTCTTCCCATTTG+TGG + 42522:2590-2609 MS.gene68888:intron 40.0%
TTGCATGTACTAGAATCTGG+TGG + 42522:1364-1383 MS.gene68888:intron 40.0%
TTGTGGTCTGCTTTGTCAAT+AGG + 42522:2235-2254 MS.gene68888:intron 40.0%
!! AAATTGATGGGCTTCTCAAC+AGG + 42522:2189-2208 MS.gene68888:CDS 40.0%
!! TATCATTTTAGTGGCGAGTG+TGG + 42522:2630-2649 MS.gene68888:intron 40.0%
AACGAACTGTCAGCTACTCA+AGG + 42522:1871-1890 MS.gene68888:CDS 45.0%
AACTGTCAGCTACTCAAGGT+CGG + 42522:1875-1894 MS.gene68888:intron 45.0%
AATTGTCTCCCCCTCACAAA+TGG - 42522:2778-2797 None:intergenic 45.0%
ACTATTCTGTGCCAAAGCAG+GGG + 42522:860-879 MS.gene68888:CDS 45.0%
AGTATATTACCTTCTGCCGG+AGG + 42522:1099-1118 MS.gene68888:intron 45.0%
AGTTCGTTGCGATCTGATTC+TGG - 42522:1859-1878 None:intergenic 45.0%
CACATGATGTGTCAGCTCAA+TGG - 42522:2452-2471 None:intergenic 45.0%
CACTATTCTGTGCCAAAGCA+GGG + 42522:859-878 MS.gene68888:CDS 45.0%
CATCAAGAAGCAAGCCTTGA+TGG + 42522:1811-1830 MS.gene68888:CDS 45.0%
CGAGCAGCAACAAGCTTAAA+AGG - 42522:2111-2130 None:intergenic 45.0%
GGTCTGCTTTGTCAATAGGA+AGG + 42522:2239-2258 MS.gene68888:intron 45.0%
GTATATTACCTTCTGCCGGA+GGG + 42522:1100-1119 MS.gene68888:intron 45.0%
TCACTATTCTGTGCCAAAGC+AGG + 42522:858-877 MS.gene68888:CDS 45.0%
TGCTCTTTGCACGTGACAAA+TGG + 42522:1291-1310 MS.gene68888:CDS 45.0%
TGGTCAACTGTGTCAACAAG+AGG - 42522:1839-1858 None:intergenic 45.0%
TGTCTTGACCATTTGTGAGG+GGG + 42522:2767-2786 MS.gene68888:CDS 45.0%
! AATTGTAGGGAGCATGACTG+TGG + 42522:2794-2813 MS.gene68888:CDS 45.0%
AGTGCTCTCATATGGCTGAC+AGG + 42522:996-1015 MS.gene68888:CDS 50.0%
CAAGATCGCGGAGTACACAT+TGG + 42522:2718-2737 MS.gene68888:CDS 50.0%
CAATGTGTACTCCGCGATCT+TGG - 42522:2720-2739 None:intergenic 50.0%
CCTTTCACGATCAATCCCTC+CGG - 42522:1118-1137 None:intergenic 50.0%
GTAGTTCTTGATGTCGGTGC+CGG + 42522:822-841 MS.gene68888:CDS 50.0%
GTCAACAAGAGGCTCCATCA+AGG - 42522:1828-1847 None:intergenic 50.0%
GTGAGGGGGAGACAATTGTA+GGG + 42522:2781-2800 MS.gene68888:CDS 50.0%
GTGCTCTCATATGGCTGACA+GGG + 42522:997-1016 MS.gene68888:CDS 50.0%
TATTTCCAGGGCCAAGATCG+CGG + 42522:2706-2725 MS.gene68888:intron 50.0%
TCATATGGCTGACAGGGCAA+AGG + 42522:1003-1022 MS.gene68888:CDS 50.0%
TGTGAGGGGGAGACAATTGT+AGG + 42522:2780-2799 MS.gene68888:CDS 50.0%
! TAGTTCTTGATGTCGGTGCC+GGG + 42522:823-842 MS.gene68888:CDS 50.0%
CAGGCGATGCAATGCTTCGA+GGG + 42522:2875-2894 MS.gene68888:CDS 55.0%
CCGGAGGGATTGATCGTGAA+AGG + 42522:1115-1134 MS.gene68888:intron 55.0%
GCAGGTCGAGTGCTCTCATA+TGG + 42522:988-1007 MS.gene68888:intron 55.0%
CGATCAATCCCTCCGGCAGA+AGG - 42522:1111-1130 None:intergenic 60.0%
GACATGTGCTGCCCCTGCTT+TGG - 42522:874-893 None:intergenic 60.0%
GCAGGCGATGCAATGCTTCG+AGG + 42522:2874-2893 MS.gene68888:CDS 60.0%
GTACTCCGCGATCTTGGCCC+TGG - 42522:2714-2733 None:intergenic 65.0%
!! CTTGATGTCGGTGCCGGGAC+TGG + 42522:828-847 MS.gene68888:CDS 65.0%


Chromosome Type Strat End Strand Name
42522 gene 747 2920 747 ID=MS.gene68888
42522 mRNA 747 2920 747 ID=MS.gene68888.t1;Parent=MS.gene68888
42522 exon 747 899 747 ID=MS.gene68888.t1.exon1;Parent=MS.gene68888.t1
42522 CDS 747 899 747 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
42522 exon 992 1055 992 ID=MS.gene68888.t1.exon2;Parent=MS.gene68888.t1
42522 CDS 992 1055 992 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
42522 exon 1174 1316 1174 ID=MS.gene68888.t1.exon3;Parent=MS.gene68888.t1
42522 CDS 1174 1316 1174 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
42522 exon 1435 1522 1435 ID=MS.gene68888.t1.exon4;Parent=MS.gene68888.t1
42522 CDS 1435 1522 1435 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
42522 exon 1777 1892 1777 ID=MS.gene68888.t1.exon5;Parent=MS.gene68888.t1
42522 CDS 1777 1892 1777 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
42522 exon 2060 2210 2060 ID=MS.gene68888.t1.exon6;Parent=MS.gene68888.t1
42522 CDS 2060 2210 2060 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
42522 exon 2715 2920 2715 ID=MS.gene68888.t1.exon7;Parent=MS.gene68888.t1
42522 CDS 2715 2920 2715 ID=cds.MS.gene68888.t1;Parent=MS.gene68888.t1
Gene Sequence

>MS.gene68888

ATGTTGAAGGACACTGTAAGAACCAAGACATATCAAAACGTTATTTATCAGAATAGGTTTTTATTCAAGAATAAAGTAGTTCTTGATGTCGGTGCCGGGACTGGAATTTTATCACTATTCTGTGCCAAAGCAGGGGCAGCACATGTCTATGCAGTGAGTTTCTGACTTTGCTTTTATCATTTTTGTGCTCTTAGTCATCATGATGATCATTTTCAATTCAACCATTTTAAATTTTGCACACGCAGGTCGAGTGCTCTCATATGGCTGACAGGGCAAAGGAAATAGTTGAAACTAATGGTTATTCTAAAGGTAGTATCATTGATATATTTGTATTATTGTATATTTAATTTTCAGTATATTACCTTCTGCCGGAGGGATTGATCGTGAAAGGCACTAACTATAATTACCATAATGTTGTTTTCTACAGTTATAACAGTTTTGAAAGGGAAGATTGAAGAACTTGAACTTCCTGTTCCCAAAGTTGATATAATCATTTCCGAATGGATGGGATATTTCTTGTTGTTTGAGAATATGTTAAATTCTGTGCTCTTTGCACGTGACAAATGGCTTGTGAGTTACATTTTCCTTACTCTTGTTGATAAGTAGTCGATTATATATTGCATGTACTAGAATCTGGTGGTCATTACTTTGACACATTCTTTTTTTTTTCTTTTTTTTTTTTGTGTAGGTGGATGATGGAGTTATTCTCCCTGATATTGCATCACTCTATCTTACCGCCATTGAAGATAAAGATTACAAAGAAGATAAAATTGAGTGTGAGCTTTTGACCTCTTGCTAAATATGATTGAGTCTTTTATACATGAAAAGATGTCCAAACCAATACTTATTTGTCTCTGAAACTGACATGCAAATTTCTTGTATTTCATTCCGAATACGGAATTGAGAAATTGTGACTGACTGCTGTTGTTGAAAACCTTGAAATTCCTAGTTTTGTTTAAACACAAATGATATTTGTGTCATCAGGTGTATTTAATTTTCTTGTTAATTATATTTCCATTTTCTATATCAGTTTGGAACAATGTATATGGATTTGACATGAGCTGCATCAAGAAGCAAGCCTTGATGGAGCCTCTTGTTGACACAGTTGACCAGAATCAGATCGCAACGAACTGTCAGCTACTCAAGGTCGGTAATATCCAGTTCTGATGATGCTTTTATGTTTAATGTACCTTGCATTGTATGTTTCATACAACAATGTCTACAATGATGAAATTTTCTTTTCATTTGGTGCAATAGTTTCTTGTTAGAATGCAAAGATTTGTATTTGTGCTGACTTGTTCATCTTTGTTCAGTCCATGGACATCTCAAAAATGTCTTCTGGAGACTGTTCATTCACAGCGCCTTTTAAGCTTGTTGCTGCTCGTGACGATTTCATTCATGCTTTTGTTGCATATTTTGATGTATCATTTACAAAGTGTCATAAATTGATGGGCTTCTCAACAGGTTAGTTCCTTTCTACTTTAGTTTTTGTGGTCTGCTTTGTCAATAGGAAGGATGAACATTGAAGTCTGTTTATTGATTCTTTCTAATAATTTGCATGTAGAAGTACGATCATATTCTTAAAAAAAGAAAATTGGGGAGGGGGGATTATTATCAATTAGTGTGGCACTAACGACAATTTTAAAACTGTTCCAAATGGGTTTTGAATTATGTCACTTGAGGTTATAAAGTCAAACTCGTACCATTGAGCTGACACATCATGTGCAAATATGATGATATTTGCTTTTAACTGAACTGAAAAGAACTGGTATTGCTTCTGAAACTATGTTTGCATGTATTTTTTCTGAAAATCGGTTGCTGAAAACTTGTACACAAGAGTTCTTTCAACAGTCTTCCCATTTGTGGTTCCGTCTTTGTTATAATATCATTTTAGTGGCGAGTGTGGATTCATTTTTATCATTTTAGTGGCGAGTGTGGATTCATTTTTGTGTACATATATATTTCCAGGGCCAAGATCGCGGAGTACACATTGGAAACAAACAGTCCTATATCTGGAAGATGTCTTGACCATTTGTGAGGGGGAGACAATTGTAGGGAGCATGACTGTGGCCCCAAATAAAAAGAATCCTCGGGATGTTGATATAATGCTCAAGTATTCATTGAATGGCAGGCGATGCAATGCTTCGAGGGTTCAGTATTACAAGATGCGTTGA

Protein sequence

>MS.gene68888.t1

MLKDTVRTKTYQNVIYQNRFLFKNKVVLDVGAGTGILSLFCAKAGAAHVYAVECSHMADRAKEIVETNGYSKVITVLKGKIEELELPVPKVDIIISEWMGYFLLFENMLNSVLFARDKWLVDDGVILPDIASLYLTAIEDKDYKEDKIEFWNNVYGFDMSCIKKQALMEPLVDTVDQNQIATNCQLLKSMDISKMSSGDCSFTAPFKLVAARDDFIHAFVAYFDVSFTKCHKLMGFSTGPRSRSTHWKQTVLYLEDVLTICEGETIVGSMTVAPNKKNPRDVDIMLKYSLNGRRCNASRVQYYKMR