AlfalfaGEDB | MS.gene29467

M. sativa cultivar XinJiangDaYe / MS.gene29467

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29467.t1	XP_003616383.1	96	126	5	0	1	126	1	126	3.60E-52	214.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29467.t1	G7KBN8	96.0	126	5	0	1	126	1	126	2.6e-52	214.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050060	MS.gene29467	0.826314	2.90E-54	-1.69E-46
MS.gene050133	MS.gene29467	-0.814935	1.20E-51	-1.69E-46
MS.gene050404	MS.gene29467	0.809776	1.60E-50	-1.69E-46
MS.gene050406	MS.gene29467	0.838838	2.27E-57	-1.69E-46
MS.gene050426	MS.gene29467	-0.839436	1.59E-57	-1.69E-46
MS.gene052011	MS.gene29467	-0.82374	1.18E-53	-1.69E-46
MS.gene052077	MS.gene29467	0.800866	1.18E-48	-1.69E-46
MS.gene052469	MS.gene29467	0.836259	1.04E-56	-1.69E-46
MS.gene052589	MS.gene29467	0.812091	5.05E-51	-1.69E-46
MS.gene052590	MS.gene29467	0.800793	1.22E-48	-1.69E-46
MS.gene053330	MS.gene29467	0.877146	7.94E-69	-1.69E-46
MS.gene054107	MS.gene29467	0.801942	7.10E-49	-1.69E-46
MS.gene05553	MS.gene29467	0.835013	2.15E-56	-1.69E-46
MS.gene056633	MS.gene29467	0.873174	1.80E-67	-1.69E-46
MS.gene056664	MS.gene29467	0.829621	4.65E-55	-1.69E-46
MS.gene057396	MS.gene29467	0.80287	4.57E-49	-1.69E-46
MS.gene058276	MS.gene29467	0.814526	1.47E-51	-1.69E-46
MS.gene058746	MS.gene29467	0.861672	8.70E-64	-1.69E-46
MS.gene059011	MS.gene29467	0.815428	9.29E-52	-1.69E-46
MS.gene059512	MS.gene29467	0.811684	6.19E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene29467.t1	MTR_5g079600	96.825	126	4	0	1	126	1	126	2.30e-86	247

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 31 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCAAAGAACTTCTTCTTCT+TGG	0.182454	5.3:-64386093	None:intergenic
ACCAAGAAGAAGAAGTTCTT+TGG	0.303733	5.3:+64386092	MS.gene29467:CDS
CGAAATTAGCTTGGTTTCCT+TGG	0.322975	5.3:-64385975	None:intergenic
CAATGGTGAGGATGCTATCT+TGG	0.372629	5.3:+64386032	MS.gene29467:CDS
TTGGAGGAAGAACATCTTGA+TGG	0.414261	5.3:+64386111	MS.gene29467:CDS
AGTACAGGTTGAAGGAGGAT+TGG	0.431371	5.3:+64386060	MS.gene29467:CDS
AGAACAACAAATAGAATTGT+TGG	0.440347	5.3:-64385851	None:intergenic
GCTCCACACAGAGGCGGAGG+AGG	0.452211	5.3:+64385934	MS.gene29467:CDS
AGCAACGGTGTCTTCGACCA+AGG	0.493021	5.3:+64385958	MS.gene29467:CDS
ACAAATAGAATTGTTGGTGT+TGG	0.499689	5.3:-64385845	None:intergenic
GGGAGAAAAGTGTCGCCCTA+TGG	0.499821	5.3:+64386132	MS.gene29467:CDS
GTTTAGCTTCGAAATTAGCT+TGG	0.508850	5.3:-64385984	None:intergenic
ACCACATAGAAAAGTGATGA+TGG	0.509265	5.3:-64385894	None:intergenic
TGGAGAAAAGTACAGGTTGA+AGG	0.517611	5.3:+64386052	MS.gene29467:CDS
CCTCACCATTGAAATAGCTC+TGG	0.520210	5.3:-64386020	None:intergenic
GAAGCTCCACACAGAGGCGG+AGG	0.525241	5.3:+64385931	MS.gene29467:CDS
AAGAAGAAGAAGTTCTTTGG+AGG	0.526017	5.3:+64386095	MS.gene29467:CDS
TTTAATTTGTTAACATGGCG+AGG	0.528637	5.3:+64385774	None:intergenic
GTTGAAGGAGGATTGGAAGA+AGG	0.543968	5.3:+64386067	MS.gene29467:CDS
TAGCACCAGAGCTATTTCAA+TGG	0.550704	5.3:+64386015	MS.gene29467:CDS
TGGAGGAAGAACATCTTGAT+GGG	0.554104	5.3:+64386112	MS.gene29467:CDS
GCTATCTTGGAGAAAAGTAC+AGG	0.563328	5.3:+64386045	MS.gene29467:CDS
AGAAAAGTACAGGTTGAAGG+AGG	0.563343	5.3:+64386055	MS.gene29467:CDS
TAGAATTGTTGGTGTTGGCA+TGG	0.575328	5.3:-64385840	None:intergenic
CTTCCTCCTCCGCCTCTGTG+TGG	0.594778	5.3:-64385937	None:intergenic
AAAGTGTCGCCCTATGGACG+AGG	0.600412	5.3:+64386138	MS.gene29467:CDS
AGAGGCGGAGGAGGAAGCAA+CGG	0.613507	5.3:+64385943	MS.gene29467:CDS
TGCATGTTAGACACAAGAAG+AGG	0.637424	5.3:-64385797	None:intergenic
CCAGAGCTATTTCAATGGTG+AGG	0.644609	5.3:+64386020	MS.gene29467:CDS
GAAGAAGCTCCACACAGAGG+CGG	0.672962	5.3:+64385928	MS.gene29467:CDS
CATGAAGAAGCTCCACACAG+AGG	0.745545	5.3:+64385925	MS.gene29467:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGAACAACAAATAGAATTGT+TGG	-	chr5.3:64385854-64385873	None:intergenic	25.0%
	ACAAATAGAATTGTTGGTGT+TGG	-	chr5.3:64385848-64385867	None:intergenic	30.0%
	AAGAAGAAGAAGTTCTTTGG+AGG	+	chr5.3:64386095-64386114	MS.gene29467:CDS	35.0%
	ACCAAGAAGAAGAAGTTCTT+TGG	+	chr5.3:64386092-64386111	MS.gene29467:CDS	35.0%
	ACCACATAGAAAAGTGATGA+TGG	-	chr5.3:64385897-64385916	None:intergenic	35.0%
	TCCAAAGAACTTCTTCTTCT+TGG	-	chr5.3:64386096-64386115	None:intergenic	35.0%
!	GTTTAGCTTCGAAATTAGCT+TGG	-	chr5.3:64385987-64386006	None:intergenic	35.0%
!!	TCCATCATCACTTTTCTATG+TGG	+	chr5.3:64385893-64385912	MS.gene29467:CDS	35.0%
	TGCATGTTAGACACAAGAAG+AGG	-	chr5.3:64385800-64385819	None:intergenic	40.0%
	TGGAGGAAGAACATCTTGAT+GGG	+	chr5.3:64386112-64386131	MS.gene29467:CDS	40.0%
	TTGGAGGAAGAACATCTTGA+TGG	+	chr5.3:64386111-64386130	MS.gene29467:CDS	40.0%
!	AGAAAAGTACAGGTTGAAGG+AGG	+	chr5.3:64386055-64386074	MS.gene29467:CDS	40.0%
!	CGAAATTAGCTTGGTTTCCT+TGG	-	chr5.3:64385978-64385997	None:intergenic	40.0%
!	GCTATCTTGGAGAAAAGTAC+AGG	+	chr5.3:64386045-64386064	MS.gene29467:CDS	40.0%
!	TAGCACCAGAGCTATTTCAA+TGG	+	chr5.3:64386015-64386034	MS.gene29467:CDS	40.0%
!	TGGAGAAAAGTACAGGTTGA+AGG	+	chr5.3:64386052-64386071	MS.gene29467:CDS	40.0%
!!	TAGAATTGTTGGTGTTGGCA+TGG	-	chr5.3:64385843-64385862	None:intergenic	40.0%
	CAATGGTGAGGATGCTATCT+TGG	+	chr5.3:64386032-64386051	MS.gene29467:CDS	45.0%
	CCTCACCATTGAAATAGCTC+TGG	-	chr5.3:64386023-64386042	None:intergenic	45.0%
	GTTGAAGGAGGATTGGAAGA+AGG	+	chr5.3:64386067-64386086	MS.gene29467:CDS	45.0%
!	AGTACAGGTTGAAGGAGGAT+TGG	+	chr5.3:64386060-64386079	MS.gene29467:CDS	45.0%
!	CCAGAGCTATTTCAATGGTG+AGG	+	chr5.3:64386020-64386039	MS.gene29467:CDS	45.0%
	CATGAAGAAGCTCCACACAG+AGG	+	chr5.3:64385925-64385944	MS.gene29467:CDS	50.0%
	AGCAACGGTGTCTTCGACCA+AGG	+	chr5.3:64385958-64385977	MS.gene29467:CDS	55.0%
	GAAGAAGCTCCACACAGAGG+CGG	+	chr5.3:64385928-64385947	MS.gene29467:CDS	55.0%
!!	AAAGTGTCGCCCTATGGACG+AGG	+	chr5.3:64386138-64386157	MS.gene29467:CDS	55.0%
!!	GGGAGAAAAGTGTCGCCCTA+TGG	+	chr5.3:64386132-64386151	MS.gene29467:CDS	55.0%
	AGAGGCGGAGGAGGAAGCAA+CGG	+	chr5.3:64385943-64385962	MS.gene29467:CDS	60.0%
	CTTCCTCCTCCGCCTCTGTG+TGG	-	chr5.3:64385940-64385959	None:intergenic	65.0%
	GAAGCTCCACACAGAGGCGG+AGG	+	chr5.3:64385931-64385950	MS.gene29467:CDS	65.0%
	GCTCCACACAGAGGCGGAGG+AGG	+	chr5.3:64385934-64385953	MS.gene29467:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	64385788	64386168	64385788	ID=MS.gene29467
chr5.3	mRNA	64385788	64386168	64385788	ID=MS.gene29467.t1;Parent=MS.gene29467
chr5.3	exon	64385788	64386168	64385788	ID=MS.gene29467.t1.exon1;Parent=MS.gene29467.t1
chr5.3	CDS	64385788	64386168	64385788	ID=cds.MS.gene29467.t1;Parent=MS.gene29467.t1

Gene Sequence

>MS.gene29467

ATGGCGAGGCCTCTTCTTGTGTCTAACATGCACTCTCTTCTTCTATTTTCATCCATGCCAACACCAACAATTCTATTTGTTGTTCTTTCAGTTTTTTCTATATTTTCCATCATCACTTTTCTATGTGGTTCTAAGAACATGAAGAAGCTCCACACAGAGGCGGAGGAGGAAGCAACGGTGTCTTCGACCAAGGAAACCAAGCTAATTTCGAAGCTAAACAGTAAAATTAGCACCAGAGCTATTTCAATGGTGAGGATGCTATCTTGGAGAAAAGTACAGGTTGAAGGAGGATTGGAAGAAGGAAACCAAGAAGAAGAAGTTCTTTGGAGGAAGAACATCTTGATGGGAGAAAAGTGTCGCCCTATGGACGAGGAAAATTAG

Protein sequence

>MS.gene29467.t1

MARPLLVSNMHSLLLFSSMPTPTILFVVLSVFSIFSIITFLCGSKNMKKLHTEAEEEATVSSTKETKLISKLNSKISTRAISMVRMLSWRKVQVEGGLEEGNQEEEVLWRKNILMGEKCRPMDEEN