AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30727

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30727.t1 MTR_6g047750 97.024 336 10 0 1 336 327 662 0.0 682
MS.gene30727.t1 MTR_7g057230 55.588 340 132 5 4 335 328 656 3.53e-127 379
MS.gene30727.t1 MTR_3g089055 39.943 353 171 9 5 336 238 570 3.28e-75 242
MS.gene30727.t1 MTR_3g089055 39.943 353 171 9 5 336 233 565 5.21e-75 241
MS.gene30727.t1 MTR_1g029420 42.671 307 151 7 41 336 300 592 2.53e-71 232
MS.gene30727.t1 MTR_2g082090 36.337 344 196 7 5 336 247 579 1.30e-60 204
MS.gene30727.t1 MTR_3g056110 36.390 349 189 9 5 336 210 542 3.79e-60 202
MS.gene30727.t1 MTR_5g097480 35.475 358 180 12 5 336 212 544 4.86e-60 201
MS.gene30727.t1 MTR_4g133660 33.429 350 196 9 5 335 211 542 3.13e-54 186
MS.gene30727.t1 MTR_2g026250 32.222 360 189 10 5 336 264 596 6.70e-54 186
MS.gene30727.t1 MTR_5g094450 34.270 356 197 10 2 336 193 532 6.34e-53 182
MS.gene30727.t1 MTR_4g074310 40.909 198 76 4 1 195 30 189 2.36e-39 139
MS.gene30727.t1 MTR_3g065980 31.290 310 183 6 45 336 243 540 1.43e-35 135
MS.gene30727.t1 MTR_8g442410 32.484 314 189 11 31 336 227 525 1.63e-34 132
MS.gene30727.t1 MTR_7g027190 32.450 302 178 9 41 336 392 673 7.45e-34 132
MS.gene30727.t1 MTR_4g074320 60.417 96 35 1 4 96 90 185 2.52e-33 122
MS.gene30727.t1 MTR_4g104020 29.609 358 211 14 2 336 181 520 6.79e-30 119
MS.gene30727.t1 MTR_4g064150 25.145 346 235 7 5 335 396 732 1.62e-28 116
MS.gene30727.t1 MTR_4g064200 25.581 344 230 9 5 335 319 649 1.16e-27 114
MS.gene30727.t1 MTR_4g064160 25.937 347 233 8 2 335 348 683 3.85e-27 112
MS.gene30727.t1 MTR_2g097350 29.153 295 185 9 51 335 359 639 1.92e-26 110
MS.gene30727.t1 MTR_2g097410 27.703 296 191 9 51 335 457 740 3.94e-26 109
MS.gene30727.t1 MTR_2g097467 27.798 277 182 6 68 335 386 653 4.67e-26 109
MS.gene30727.t1 MTR_2g097463 27.076 277 184 6 68 335 387 654 4.86e-26 109
MS.gene30727.t1 MTR_4g097080 26.257 358 222 11 2 336 165 503 1.01e-25 107
MS.gene30727.t1 MTR_2g097390 26.370 292 197 6 51 335 360 640 6.11e-25 105
MS.gene30727.t1 MTR_4g102790 31.481 270 164 10 75 335 404 661 1.17e-24 105
MS.gene30727.t1 MTR_2g097310 26.712 292 196 7 51 335 357 637 2.98e-24 103
MS.gene30727.t1 MTR_2g097473 26.812 276 186 5 68 335 386 653 3.04e-24 103
MS.gene30727.t1 MTR_7g062120 24.542 273 184 8 75 336 479 740 2.27e-22 98.6
MS.gene30727.t1 MTR_7g074650 29.693 293 176 10 51 336 523 792 5.65e-22 97.4
MS.gene30727.t1 MTR_4g064180 22.832 346 243 7 3 333 287 623 1.03e-21 96.3
MS.gene30727.t1 MTR_4g064120 22.767 347 244 8 2 333 286 623 1.27e-21 96.3
MS.gene30727.t1 MTR_2g097380 24.658 292 187 6 51 335 295 560 4.35e-21 94.4
MS.gene30727.t1 MTR_2g034260 28.826 281 171 9 69 336 320 584 5.86e-19 88.2
MS.gene30727.t1 MTR_2g034250 28.520 277 166 10 75 336 326 585 1.41e-18 87.0
MS.gene30727.t1 MTR_5g015950 24.031 387 233 9 2 335 67 445 2.21e-18 85.9
MS.gene30727.t1 MTR_3g053270 24.031 387 233 9 2 335 67 445 2.21e-18 85.9
MS.gene30727.t1 MTR_4g076140 25.496 353 199 10 41 336 124 469 6.75e-18 84.7
MS.gene30727.t1 MTR_1g069725 25.658 304 199 10 39 335 179 462 1.35e-17 83.6
MS.gene30727.t1 MTR_2g034280 29.091 275 166 10 75 336 317 575 3.58e-17 82.8
MS.gene30727.t1 MTR_3g022830 30.337 178 105 5 170 336 267 436 6.01e-17 81.6
MS.gene30727.t1 MTR_5g009080 22.923 349 196 7 50 336 141 478 1.06e-16 81.3
MS.gene30727.t1 MTR_7g109580 27.574 272 171 8 75 336 299 554 2.95e-16 80.1
MS.gene30727.t1 MTR_1g096030 27.520 367 201 14 7 336 121 459 5.38e-16 79.0
MS.gene30727.t1 MTR_4g076020 26.936 297 175 12 47 336 168 429 8.33e-14 72.4
MS.gene30727.t1 MTR_5g015490 23.986 296 204 7 53 336 204 490 1.03e-13 72.0
MS.gene30727.t1 MTR_3g072710 26.433 314 188 13 41 335 210 499 7.79e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30727.t1 AT5G52510 55.650 354 125 8 2 336 300 640 7.91e-123 367
MS.gene30727.t1 AT1G21450 37.815 357 180 10 2 336 257 593 2.32e-64 214
MS.gene30727.t1 AT2G04890 36.628 344 188 10 5 336 88 413 1.27e-62 205
MS.gene30727.t1 AT5G48150 39.752 322 164 8 26 336 188 490 5.27e-61 202
MS.gene30727.t1 AT5G48150 39.752 322 164 8 26 336 188 490 5.27e-61 202
MS.gene30727.t1 AT5G48150 39.752 322 164 8 26 336 188 490 5.27e-61 202
MS.gene30727.t1 AT5G48150 39.752 322 164 8 26 336 188 490 5.27e-61 202
MS.gene30727.t1 AT1G50600 34.582 347 198 8 5 336 194 526 2.10e-53 184
MS.gene30727.t1 AT1G50600 34.659 352 191 9 5 336 265 597 5.69e-53 184
MS.gene30727.t1 AT1G50600 34.582 347 198 8 5 336 269 601 5.79e-53 184
MS.gene30727.t1 AT4G17230 33.718 347 201 8 5 336 193 525 2.04e-51 178
MS.gene30727.t1 AT4G17230 33.718 347 201 8 5 336 193 525 2.04e-51 178
MS.gene30727.t1 AT2G01570 33.446 296 182 6 45 336 297 581 3.30e-37 140
MS.gene30727.t1 AT3G03450 32.215 298 184 6 45 336 260 545 1.21e-36 139
MS.gene30727.t1 AT1G14920 30.675 326 200 8 18 336 223 529 4.05e-36 137
MS.gene30727.t1 AT1G66350 31.987 297 179 8 45 336 228 506 1.46e-34 132
MS.gene30727.t1 AT5G17490 31.741 293 180 7 50 336 238 516 3.48e-33 129
MS.gene30727.t1 AT5G59450 25.937 347 232 7 5 336 262 598 2.98e-32 127
MS.gene30727.t1 AT2G29060 29.333 300 193 8 47 335 363 654 1.09e-31 125
MS.gene30727.t1 AT2G29060 29.333 300 193 8 47 335 400 691 1.13e-31 125
MS.gene30727.t1 AT1G07530 27.575 301 195 9 47 335 475 764 1.55e-30 122
MS.gene30727.t1 AT2G29065 27.541 305 202 8 41 335 332 627 4.79e-28 115
MS.gene30727.t1 AT3G46600 24.716 352 232 8 2 336 36 371 7.55e-28 112
MS.gene30727.t1 AT3G46600 24.716 352 232 8 2 336 212 547 7.42e-27 111
MS.gene30727.t1 AT3G46600 24.716 352 232 8 2 336 244 579 1.19e-26 110
MS.gene30727.t1 AT1G07520 23.932 351 242 9 2 335 186 528 1.13e-25 107
MS.gene30727.t1 AT1G07520 23.932 351 242 9 2 335 186 528 1.13e-25 107
MS.gene30727.t1 AT1G07520 23.932 351 242 9 2 335 351 693 2.90e-25 107
MS.gene30727.t1 AT1G07520 23.932 351 242 9 2 335 350 692 3.18e-25 107
MS.gene30727.t1 AT5G66770 31.667 300 176 11 51 336 300 584 3.06e-24 103
MS.gene30727.t1 AT3G50650 32.343 303 174 14 51 336 254 542 5.07e-24 103
MS.gene30727.t1 AT2G37650 25.733 307 208 7 38 336 407 701 1.02e-23 102
MS.gene30727.t1 AT2G37650 25.733 307 208 7 38 336 419 713 1.21e-23 102
MS.gene30727.t1 AT3G54220 30.068 296 177 12 47 335 377 649 1.02e-22 99.4
MS.gene30727.t1 AT3G49950 25.141 354 237 10 2 335 62 407 3.69e-21 94.0
MS.gene30727.t1 AT4G37650 26.144 306 190 9 53 336 238 529 7.27e-17 81.6
MS.gene30727.t1 AT1G50420 32.258 155 94 3 188 336 330 479 1.04e-16 81.3
MS.gene30727.t1 AT1G55580 24.415 299 203 8 51 336 157 445 3.17e-16 79.7
MS.gene30727.t1 AT1G63100 28.505 214 138 8 75 282 388 592 2.27e-14 74.3
MS.gene30727.t1 AT1G63100 28.505 214 138 8 75 282 363 567 2.74e-14 74.3
MS.gene30727.t1 AT4G08250 26.045 311 195 12 41 335 185 476 3.33e-13 70.5

Find 79 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTGCAAATCTCGCAATTC+TGG 0.228552 6.2:+41832471 MS.gene30727:CDS
TGAGTTGACTCGCGAGTTAT+TGG 0.276169 6.2:+41832756 MS.gene30727:CDS
TTGTTTCAAGGTTGGTTTCA+TGG 0.293135 6.2:+41832450 MS.gene30727:CDS
CCGGTTGTTGCGGAATTGTT+CGG 0.301036 6.2:+41832382 MS.gene30727:CDS
TGGGATTTGCTTTGGTTGGA+TGG 0.327746 6.2:+41833260 MS.gene30727:CDS
CGGTTGTTGCGGAATTGTTC+GGG 0.328355 6.2:+41832383 MS.gene30727:CDS
GAATCTGAATTCGATGAATT+CGG 0.332765 6.2:+41832294 None:intergenic
CACTCACGGTTGCTTCTGTT+TGG 0.353225 6.2:+41833289 MS.gene30727:CDS
AGCGCGTGAGGCGGCGCGTG+AGG 0.389779 6.2:+41832588 MS.gene30727:CDS
GGCGCGTGTTGCAGAGTCAT+GGG 0.403439 6.2:+41832957 MS.gene30727:CDS
GAGTTGACTCGCGAGTTATT+GGG 0.414095 6.2:+41832757 MS.gene30727:CDS
GCGTGAGGGGTTTAAGTTGA+AGG 0.417985 6.2:+41832603 MS.gene30727:CDS
ATGGTGGGATTTGCTTTGGT+TGG 0.418527 6.2:+41833256 MS.gene30727:CDS
ATTAAGTCGCGACTCAATAT+CGG 0.419497 6.2:+41833186 MS.gene30727:CDS
GCGCGTGAGGCGGCGCGTGA+GGG 0.419954 6.2:+41832589 MS.gene30727:CDS
CAAGCGGAGAAGCTAAGAAT+TGG 0.420635 6.2:+41832691 MS.gene30727:CDS
ATGGCGCGCAAGGATGAGTA+TGG 0.425941 6.2:+41833119 MS.gene30727:CDS
GGAAAACTCGGTTTGTTTCA+AGG 0.430920 6.2:+41832438 MS.gene30727:CDS
TCAACGGTTCACGAATTTCA+TGG 0.443579 6.2:+41832318 MS.gene30727:CDS
TGGCGCGTGTTGCAGAGTCA+TGG 0.446031 6.2:+41832956 MS.gene30727:CDS
GGGATTTGCTTTGGTTGGAT+GGG 0.446689 6.2:+41833261 MS.gene30727:CDS
TCCGTTGACACACTTTCATC+CGG 0.449000 6.2:-41832835 None:intergenic
TAACGAGTCGCGAGTTAACT+CGG 0.451482 6.2:+41833212 MS.gene30727:CDS
CGCTTTATGTGACTCGGTCG+AGG 0.459461 6.2:+41832990 MS.gene30727:CDS
GAGAATGGTGGGATTTGCTT+TGG 0.460359 6.2:+41833252 MS.gene30727:CDS
CGCGCAAGGATGAGTATGGC+GGG 0.461869 6.2:+41833123 MS.gene30727:CDS
GTATAAGCTGAATAGAATTC+CGG 0.464091 6.2:+41832816 MS.gene30727:CDS
GTTGAAGGTTGTTGCGGTGG+CGG 0.467280 6.2:+41832618 MS.gene30727:CDS
CCGAACAATTCCGCAACAAC+CGG 0.477361 6.2:-41832382 None:intergenic
TTTGAAATCGAGGATGAATC+CGG 0.479465 6.2:+41832348 MS.gene30727:CDS
GTTGTTGCGGTGGCGGAGAA+CGG 0.484971 6.2:+41832625 MS.gene30727:CDS
CTCCTTCCTCAATCTTGACT+CGG 0.486598 6.2:-41833023 None:intergenic
AACTCGGTTTGTTTCAAGGT+TGG 0.489425 6.2:+41832442 MS.gene30727:CDS
CGCGTGAGGCGGCGCGTGAG+GGG 0.490221 6.2:+41832590 MS.gene30727:CDS
TCAGTTGTTGTTGGAAAACT+CGG 0.502026 6.2:+41832426 MS.gene30727:CDS
CGCGCTCAAAGCGCGTGAGG+CGG 0.514899 6.2:+41832579 MS.gene30727:CDS
TGAAAATCCACCGGTTGTTG+CGG 0.514967 6.2:+41832372 MS.gene30727:CDS
TTCCTCAATCTTGACTCGGT+CGG 0.527860 6.2:-41833019 None:intergenic
TGAGTCGACTCAGTTGTTGT+TGG 0.533088 6.2:+41832417 MS.gene30727:CDS
CTTGGTGGCTTGTGAAGGTA+GGG 0.547488 6.2:+41833065 MS.gene30727:CDS
TTATTCCGCTTTATGTGACT+CGG 0.559717 6.2:+41832984 MS.gene30727:CDS
GAAACTCGCGCCGCGTGTTG+TGG 0.562154 6.2:+41832891 MS.gene30727:CDS
TCGAAAATTGTGTAACTTGG+TGG 0.564401 6.2:+41833050 MS.gene30727:CDS
GATGCTGCATTTGAAGAAAA+CGG 0.565293 6.2:+41832493 MS.gene30727:CDS
GACCGAGTCAAGATTGAGGA+AGG 0.567652 6.2:+41833021 MS.gene30727:CDS
AACGAGTCGCGAGTTAACTC+GGG 0.568702 6.2:+41833213 MS.gene30727:CDS
TTTCGAGTTTCGTGTTGTGT+CGG 0.573944 6.2:+41832714 MS.gene30727:CDS
TCCGGATGAAAGTGTGTCAA+CGG 0.577258 6.2:+41832834 MS.gene30727:CDS
ACTTGGTGGCTTGTGAAGGT+AGG 0.578105 6.2:+41833064 MS.gene30727:CDS
GTTTAAGTTGAAGGTTGTTG+CGG 0.578524 6.2:+41832612 MS.gene30727:CDS
CTCAGTGTGAAAGAAGAGAA+TGG 0.580110 6.2:+41833237 MS.gene30727:CDS
GTGTGAAAGAAGAGAATGGT+GGG 0.588009 6.2:+41833241 MS.gene30727:CDS
CTCGACCGAGTCACATAAAG+CGG 0.588841 6.2:-41832989 None:intergenic
TGGTGAGAGCTTTGAAATCG+AGG 0.590617 6.2:+41832338 MS.gene30727:CDS
TAAGTTGAAGGTTGTTGCGG+TGG 0.592167 6.2:+41832615 MS.gene30727:CDS
CTCCGACCGAGTCAAGATTG+AGG 0.608118 6.2:+41833017 MS.gene30727:CDS
AACAATTCCGCAACAACCGG+TGG 0.610591 6.2:-41832379 None:intergenic
TCACGCGCTCAAAGCGCGTG+AGG 0.611087 6.2:+41832576 MS.gene30727:CDS
TGTAACTTGGTGGCTTGTGA+AGG 0.611397 6.2:+41833060 MS.gene30727:CDS
TAGCGGTGGGTGAGATGTTG+AGG 0.615342 6.2:+41832665 MS.gene30727:CDS
CGCCGCAAAAGTGTGTCACG+TGG 0.618368 6.2:-41832862 None:intergenic
GCGCGTGTTGCAGAGTCATG+GGG 0.623740 6.2:+41832958 MS.gene30727:CDS
GTTGCGGTGGCGGAGAACGG+CGG 0.627141 6.2:+41832628 MS.gene30727:CDS
AAGGTAGGGATCGTGTCGAA+AGG 0.628894 6.2:+41833079 MS.gene30727:CDS
GCCGCGTGTTGTGGTTGTTG+TGG 0.629120 6.2:+41832900 MS.gene30727:CDS
ATTCGATGAATTCGGATCAA+CGG 0.629816 6.2:+41832302 None:intergenic
TCCACAACAACCACAACACG+CGG 0.636009 6.2:-41832901 None:intergenic
GAGTCGAAAATTGTGTAACT+TGG 0.636503 6.2:+41833047 MS.gene30727:CDS
GAATCCGGTTGAAAATCCAC+CGG 0.645288 6.2:+41832363 MS.gene30727:CDS
GCGCGCAAGGATGAGTATGG+CGG 0.653133 6.2:+41833122 MS.gene30727:CDS
TGAGATGTTGAGGCTTCAAG+CGG 0.656124 6.2:+41832675 MS.gene30727:CDS
TTGGATGGGAAGAACACTCA+CGG 0.668699 6.2:+41833275 MS.gene30727:CDS
GGATCGTGTCGAAAGGTGTG+AGG 0.671431 6.2:+41833086 MS.gene30727:CDS
GCGCAAGGATGAGTATGGCG+GGG 0.685055 6.2:+41833124 MS.gene30727:CDS
CGGTGATGATAGAGTGATAG+CGG 0.689852 6.2:+41832648 MS.gene30727:CDS
GAGTCGCGACTTAATCGACT+CGG 0.695155 6.2:-41833178 None:intergenic
AGTGTGAAAGAAGAGAATGG+TGG 0.698819 6.2:+41833240 MS.gene30727:CDS
TGATGATAGAGTGATAGCGG+TGG 0.709647 6.2:+41832651 MS.gene30727:CDS
GATGATAGAGTGATAGCGGT+GGG 0.720839 6.2:+41832652 MS.gene30727:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTGTAGTTGATTTTGAAAT+TGG + chr6.2:41832526-41832545 MS.gene30727:CDS 25.0%
GTATAAGCTGAATAGAATTC+CGG + chr6.2:41832816-41832835 MS.gene30727:CDS 30.0%
!!! AGTTGATTTTGAAATTGGAC+AGG + chr6.2:41832531-41832550 MS.gene30727:CDS 30.0%
!!! GTTGATTTTGAAATTGGACA+GGG + chr6.2:41832532-41832551 MS.gene30727:CDS 30.0%
ATTAAGTCGCGACTCAATAT+CGG + chr6.2:41833186-41833205 MS.gene30727:CDS 35.0%
GAGTCGAAAATTGTGTAACT+TGG + chr6.2:41833047-41833066 MS.gene30727:CDS 35.0%
GATGCTGCATTTGAAGAAAA+CGG + chr6.2:41832493-41832512 MS.gene30727:CDS 35.0%
TCGAAAATTGTGTAACTTGG+TGG + chr6.2:41833050-41833069 MS.gene30727:CDS 35.0%
TTATTCCGCTTTATGTGACT+CGG + chr6.2:41832984-41833003 MS.gene30727:CDS 35.0%
! TCAGTTGTTGTTGGAAAACT+CGG + chr6.2:41832426-41832445 MS.gene30727:CDS 35.0%
! TTTGAAATCGAGGATGAATC+CGG + chr6.2:41832348-41832367 MS.gene30727:CDS 35.0%
!! GTTTAAGTTGAAGGTTGTTG+CGG + chr6.2:41832612-41832631 MS.gene30727:CDS 35.0%
!! TTGTTTCAAGGTTGGTTTCA+TGG + chr6.2:41832450-41832469 MS.gene30727:CDS 35.0%
AGTGTGAAAGAAGAGAATGG+TGG + chr6.2:41833240-41833259 MS.gene30727:CDS 40.0%
CTCAGTGTGAAAGAAGAGAA+TGG + chr6.2:41833237-41833256 MS.gene30727:CDS 40.0%
GTGTGAAAGAAGAGAATGGT+GGG + chr6.2:41833241-41833260 MS.gene30727:CDS 40.0%
TCAACGGTTCACGAATTTCA+TGG + chr6.2:41832318-41832337 MS.gene30727:CDS 40.0%
! AACTCGGTTTGTTTCAAGGT+TGG + chr6.2:41832442-41832461 MS.gene30727:CDS 40.0%
! GGAAAACTCGGTTTGTTTCA+AGG + chr6.2:41832438-41832457 MS.gene30727:CDS 40.0%
!! TTTCGAGTTTCGTGTTGTGT+CGG + chr6.2:41832714-41832733 MS.gene30727:CDS 40.0%
!!! TCGGCAACATTTTGACTCAT+TGG - chr6.2:41833162-41833181 None:intergenic 40.0%
CAAGCGGAGAAGCTAAGAAT+TGG + chr6.2:41832691-41832710 MS.gene30727:CDS 45.0%
CGGTGATGATAGAGTGATAG+CGG + chr6.2:41832648-41832667 MS.gene30727:CDS 45.0%
CTCCTTCCTCAATCTTGACT+CGG - chr6.2:41833026-41833045 None:intergenic 45.0%
GAATCCGGTTGAAAATCCAC+CGG + chr6.2:41832363-41832382 MS.gene30727:CDS 45.0%
TAACGAGTCGCGAGTTAACT+CGG + chr6.2:41833212-41833231 MS.gene30727:CDS 45.0%
TCCGTTGACACACTTTCATC+CGG - chr6.2:41832838-41832857 None:intergenic 45.0%
TGAGATGTTGAGGCTTCAAG+CGG + chr6.2:41832675-41832694 MS.gene30727:CDS 45.0%
TTGGATGGGAAGAACACTCA+CGG + chr6.2:41833275-41833294 MS.gene30727:CDS 45.0%
! ATCCACGTGACACACTTTTG+CGG + chr6.2:41832860-41832879 MS.gene30727:CDS 45.0%
! ATGGTGGGATTTGCTTTGGT+TGG + chr6.2:41833256-41833275 MS.gene30727:CDS 45.0%
! GAGAATGGTGGGATTTGCTT+TGG + chr6.2:41833252-41833271 MS.gene30727:CDS 45.0%
! GGGATTTGCTTTGGTTGGAT+GGG + chr6.2:41833261-41833280 MS.gene30727:CDS 45.0%
! TAAGTTGAAGGTTGTTGCGG+TGG + chr6.2:41832615-41832634 MS.gene30727:CDS 45.0%
! TCCGGATGAAAGTGTGTCAA+CGG + chr6.2:41832834-41832853 MS.gene30727:CDS 45.0%
! TGAAAATCCACCGGTTGTTG+CGG + chr6.2:41832372-41832391 MS.gene30727:CDS 45.0%
! TGAGTCGACTCAGTTGTTGT+TGG + chr6.2:41832417-41832436 MS.gene30727:CDS 45.0%
! TGGGATTTGCTTTGGTTGGA+TGG + chr6.2:41833260-41833279 MS.gene30727:CDS 45.0%
! TGTAACTTGGTGGCTTGTGA+AGG + chr6.2:41833060-41833079 MS.gene30727:CDS 45.0%
!! ACAACCGGTGGATTTTCAAC+CGG - chr6.2:41832370-41832389 None:intergenic 45.0%
!! GAGTTGACTCGCGAGTTATT+GGG + chr6.2:41832757-41832776 MS.gene30727:CDS 45.0%
!! GATGATAGAGTGATAGCGGT+GGG + chr6.2:41832652-41832671 MS.gene30727:CDS 45.0%
!! GTCGAAAGGTGTGAGGTTTT+CGG + chr6.2:41833093-41833112 MS.gene30727:CDS 45.0%
!! TGAGTTGACTCGCGAGTTAT+TGG + chr6.2:41832756-41832775 MS.gene30727:CDS 45.0%
!! TGATGATAGAGTGATAGCGG+TGG + chr6.2:41832651-41832670 MS.gene30727:CDS 45.0%
!! TGGTGAGAGCTTTGAAATCG+AGG + chr6.2:41832338-41832357 MS.gene30727:CDS 45.0%
!! TTCCTCAATCTTGACTCGGT+CGG - chr6.2:41833022-41833041 None:intergenic 45.0%
!! TTGTAATACCGCGCCGTTTT+TGG + chr6.2:41832936-41832955 MS.gene30727:CDS 45.0%
AACAATTCCGCAACAACCGG+TGG - chr6.2:41832382-41832401 None:intergenic 50.0%
AACGAGTCGCGAGTTAACTC+GGG + chr6.2:41833213-41833232 MS.gene30727:CDS 50.0%
AAGGTAGGGATCGTGTCGAA+AGG + chr6.2:41833079-41833098 MS.gene30727:CDS 50.0%
CACTCACGGTTGCTTCTGTT+TGG + chr6.2:41833289-41833308 MS.gene30727:CDS 50.0%
CCGAACAATTCCGCAACAAC+CGG - chr6.2:41832385-41832404 None:intergenic 50.0%
CCGGTTGTTGCGGAATTGTT+CGG + chr6.2:41832382-41832401 MS.gene30727:CDS 50.0%
CGGTTGTTGCGGAATTGTTC+GGG + chr6.2:41832383-41832402 MS.gene30727:CDS 50.0%
CTCGACCGAGTCACATAAAG+CGG - chr6.2:41832992-41833011 None:intergenic 50.0%
GACCGAGTCAAGATTGAGGA+AGG + chr6.2:41833021-41833040 MS.gene30727:CDS 50.0%
GAGTCGCGACTTAATCGACT+CGG - chr6.2:41833181-41833200 None:intergenic 50.0%
GGCTGCAAATCTCGCAATTC+TGG + chr6.2:41832471-41832490 MS.gene30727:CDS 50.0%
TCCACAACAACCACAACACG+CGG - chr6.2:41832904-41832923 None:intergenic 50.0%
TCTGCAACACGCGCCAAAAA+CGG - chr6.2:41832952-41832971 None:intergenic 50.0%
! ACTTGGTGGCTTGTGAAGGT+AGG + chr6.2:41833064-41833083 MS.gene30727:CDS 50.0%
! CTTGGTGGCTTGTGAAGGTA+GGG + chr6.2:41833065-41833084 MS.gene30727:CDS 50.0%
! GGTGTGAGGTTTTCGGCAAA+TGG + chr6.2:41833100-41833119 MS.gene30727:CDS 50.0%
!! GCGTGAGGGGTTTAAGTTGA+AGG + chr6.2:41832603-41832622 MS.gene30727:CDS 50.0%
ATGGCGCGCAAGGATGAGTA+TGG + chr6.2:41833119-41833138 MS.gene30727:CDS 55.0%
CTCCGACCGAGTCAAGATTG+AGG + chr6.2:41833017-41833036 MS.gene30727:CDS 55.0%
GTTGAAGGTTGTTGCGGTGG+CGG + chr6.2:41832618-41832637 MS.gene30727:CDS 55.0%
TAGCGGTGGGTGAGATGTTG+AGG + chr6.2:41832665-41832684 MS.gene30727:CDS 55.0%
! GGATCGTGTCGAAAGGTGTG+AGG + chr6.2:41833086-41833105 MS.gene30727:CDS 55.0%
! TTTTCGGCAAATGGCGCGCA+AGG + chr6.2:41833109-41833128 MS.gene30727:CDS 55.0%
!! CGCTTTATGTGACTCGGTCG+AGG + chr6.2:41832990-41833009 MS.gene30727:CDS 55.0%
AACACGCGCCAAAAACGGCG+CGG - chr6.2:41832947-41832966 None:intergenic 60.0%
CGCGCAAGGATGAGTATGGC+GGG + chr6.2:41833123-41833142 MS.gene30727:CDS 60.0%
GCCGCGTGTTGTGGTTGTTG+TGG + chr6.2:41832900-41832919 MS.gene30727:CDS 60.0%
GCGCAAGGATGAGTATGGCG+GGG + chr6.2:41833124-41833143 MS.gene30727:CDS 60.0%
GCGCGCAAGGATGAGTATGG+CGG + chr6.2:41833122-41833141 MS.gene30727:CDS 60.0%
GCGCGTGTTGCAGAGTCATG+GGG + chr6.2:41832958-41832977 MS.gene30727:CDS 60.0%
GGCGCGTGTTGCAGAGTCAT+GGG + chr6.2:41832957-41832976 MS.gene30727:CDS 60.0%
TGGCGCGTGTTGCAGAGTCA+TGG + chr6.2:41832956-41832975 MS.gene30727:CDS 60.0%
! CGCCGCAAAAGTGTGTCACG+TGG - chr6.2:41832865-41832884 None:intergenic 60.0%
!! GTTGTTGCGGTGGCGGAGAA+CGG + chr6.2:41832625-41832644 MS.gene30727:CDS 60.0%
GAAACTCGCGCCGCGTGTTG+TGG + chr6.2:41832891-41832910 MS.gene30727:CDS 65.0%
TCACGCGCTCAAAGCGCGTG+AGG + chr6.2:41832576-41832595 MS.gene30727:CDS 65.0%
CGCGCTCAAAGCGCGTGAGG+CGG + chr6.2:41832579-41832598 MS.gene30727:CDS 70.0%
!! GTTGCGGTGGCGGAGAACGG+CGG + chr6.2:41832628-41832647 MS.gene30727:CDS 70.0%
! AGCGCGTGAGGCGGCGCGTG+AGG + chr6.2:41832588-41832607 MS.gene30727:CDS 80.0%
! CGCGTGAGGCGGCGCGTGAG+GGG + chr6.2:41832590-41832609 MS.gene30727:CDS 80.0%
! GCGCGTGAGGCGGCGCGTGA+GGG + chr6.2:41832589-41832608 MS.gene30727:CDS 80.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 41832307 41833317 41832307 ID=MS.gene30727
chr6.2 mRNA 41832307 41833317 41832307 ID=MS.gene30727.t1;Parent=MS.gene30727
chr6.2 exon 41832307 41833317 41832307 ID=MS.gene30727.t1.exon1;Parent=MS.gene30727.t1
chr6.2 CDS 41832307 41833317 41832307 ID=cds.MS.gene30727.t1;Parent=MS.gene30727.t1
Gene Sequence

>MS.gene30727

ATGAATTCGGATCAACGGTTCACGAATTTCATGGTGAGAGCTTTGAAATCGAGGATGAATCCGGTTGAAAATCCACCGGTTGTTGCGGAATTGTTCGGGAGAGAACATGCTGAGTCGACTCAGTTGTTGTTGGAAAACTCGGTTTGTTTCAAGGTTGGTTTCATGGCTGCAAATCTCGCAATTCTGGATGCTGCATTTGAAGAAAACGGTAACAGTTTCTGTGTAGTTGATTTTGAAATTGGACAGGGAAAACAGTATGTGAATCTTCTTCACGCGCTCAAAGCGCGTGAGGCGGCGCGTGAGGGGTTTAAGTTGAAGGTTGTTGCGGTGGCGGAGAACGGCGGTGATGATAGAGTGATAGCGGTGGGTGAGATGTTGAGGCTTCAAGCGGAGAAGCTAAGAATTGGTTTCGAGTTTCGTGTTGTGTCGGTTTCTCAGAGACAAGTCTATGAGTTGACTCGCGAGTTATTGGGATGTGATGTTGAAGAGACAGTAATTGTGAATTTCGCGTATAAGCTGAATAGAATTCCGGATGAAAGTGTGTCAACGGAGAATCCACGTGACACACTTTTGCGGCGCGTGAAGAAACTCGCGCCGCGTGTTGTGGTTGTTGTGGAGCAAGAAATGAATTGTAATACCGCGCCGTTTTTGGCGCGTGTTGCAGAGTCATGGGGTTATTATTCCGCTTTATGTGACTCGGTCGAGGAAAACTCCGACCGAGTCAAGATTGAGGAAGGAGTGAGTCGAAAATTGTGTAACTTGGTGGCTTGTGAAGGTAGGGATCGTGTCGAAAGGTGTGAGGTTTTCGGCAAATGGCGCGCAAGGATGAGTATGGCGGGGTTTAAGATGAAACCAATGAGTCAAAATGTTGCCGAGTCGATTAAGTCGCGACTCAATATCGGTAATAACGAGTCGCGAGTTAACTCGGGACTCAGTGTGAAAGAAGAGAATGGTGGGATTTGCTTTGGTTGGATGGGAAGAACACTCACGGTTGCTTCTGTTTGGCGTTAA
Protein sequence

>MS.gene30727.t1

MNSDQRFTNFMVRALKSRMNPVENPPVVAELFGREHAESTQLLLENSVCFKVGFMAANLAILDAAFEENGNSFCVVDFEIGQGKQYVNLLHALKAREAAREGFKLKVVAVAENGGDDRVIAVGEMLRLQAEKLRIGFEFRVVSVSQRQVYELTRELLGCDVEETVIVNFAYKLNRIPDESVSTENPRDTLLRRVKKLAPRVVVVVEQEMNCNTAPFLARVAESWGYYSALCDSVEENSDRVKIEEGVSRKLCNLVACEGRDRVERCEVFGKWRARMSMAGFKMKPMSQNVAESIKSRLNIGNNESRVNSGLSVKEENGGICFGWMGRTLTVASVWR