Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32342.t1 | XP_013466382.1 | 85.5 | 62 | 9 | 0 | 1 | 62 | 1 | 62 | 1.20E-08 | 68.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32342.t1 | A0A072VG86 | 85.5 | 62 | 9 | 0 | 1 | 62 | 1 | 62 | 8.6e-09 | 68.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene32342 | 0.804879 | 1.75E-49 | -1.69E-46 |
| MS.gene051996 | MS.gene32342 | 0.804086 | 2.56E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene32342 | 0.803523 | 3.35E-49 | -1.69E-46 |
| MS.gene054138 | MS.gene32342 | 0.96247 | 6.47E-121 | -1.69E-46 |
| MS.gene054146 | MS.gene32342 | 0.801105 | 1.05E-48 | -1.69E-46 |
| MS.gene054742 | MS.gene32342 | 0.831612 | 1.51E-55 | -1.69E-46 |
| MS.gene056330 | MS.gene32342 | 0.853265 | 2.68E-61 | -1.69E-46 |
| MS.gene056920 | MS.gene32342 | 0.809222 | 2.10E-50 | -1.69E-46 |
| MS.gene056922 | MS.gene32342 | 0.819745 | 9.88E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene32342 | 0.821296 | 4.35E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene32342 | 0.833711 | 4.56E-56 | -1.69E-46 |
| MS.gene057261 | MS.gene32342 | 0.809719 | 1.65E-50 | -1.69E-46 |
| MS.gene059039 | MS.gene32342 | 0.80415 | 2.48E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene32342 | 0.818434 | 1.96E-52 | -1.69E-46 |
| MS.gene060578 | MS.gene32342 | 0.801975 | 6.99E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene06050 | MS.gene32342 | PPI |
| MS.gene048906 | MS.gene32342 | PPI |
| MS.gene55776 | MS.gene32342 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32342.t1 | MTR_1g028610 | 95.161 | 62 | 3 | 0 | 1 | 62 | 1 | 62 | 6.98e-29 | 97.4 |
| MS.gene32342.t1 | MTR_3g090830 | 77.966 | 59 | 11 | 1 | 4 | 62 | 7 | 63 | 9.14e-25 | 87.0 |
| MS.gene32342.t1 | MTR_6g086390 | 41.667 | 60 | 35 | 0 | 3 | 62 | 5 | 64 | 2.74e-11 | 53.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32342.t1 | AT2G46330 | 66.000 | 50 | 15 | 1 | 13 | 62 | 15 | 62 | 6.39e-16 | 65.1 |
| MS.gene32342.t1 | AT3G61640 | 57.143 | 56 | 22 | 1 | 7 | 62 | 9 | 62 | 1.68e-15 | 63.9 |
| MS.gene32342.t1 | AT5G53250 | 76.923 | 39 | 9 | 0 | 24 | 62 | 25 | 63 | 9.63e-15 | 61.6 |
Find 11 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGCAAGGCCACCAGCATT+AGG | 0.444107 | 1.4:+14011102 | None:intergenic |
| TCACTTGTGGGAGCAGGAGC+AGG | 0.473725 | 1.4:+14011961 | None:intergenic |
| TTCTTCACAGTCTGTTGCTC+CGG | 0.477404 | 1.4:-14011986 | MS.gene32342:CDS |
| GTGGGAGCAGGAGCAGGAGC+CGG | 0.500195 | 1.4:+14011967 | None:intergenic |
| TTAGGACATATGCTATTGCT+TGG | 0.507960 | 1.4:+14011120 | None:intergenic |
| TTACCATCACTTGTGGGAGC+AGG | 0.532408 | 1.4:+14011955 | None:intergenic |
| GCTCCTGCTCCCACAAGTGA+TGG | 0.550760 | 1.4:-14011958 | MS.gene32342:intron |
| AATAGCATATGTCCTAATGC+TGG | 0.576700 | 1.4:-14011114 | MS.gene32342:CDS |
| AGCATATGTCCTAATGCTGG+TGG | 0.610729 | 1.4:-14011111 | MS.gene32342:CDS |
| AAGATGCGTAAATAAGACCA+AGG | 0.685667 | 1.4:+14012022 | None:intergenic |
| AATGATGTAAGTGAGAAGCA+AGG | 0.690125 | 1.4:+14011088 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAATCTAGACTATATT+GGG | - | chr1.4:14011651-14011670 | MS.gene32342:intron | 15.0% |
| !! | TCAATCAAAAACTTTAAATA+AGG | - | chr1.4:14011452-14011471 | MS.gene32342:intron | 15.0% |
| !! | TGTTATTATTAACTGATAAT+AGG | + | chr1.4:14011805-14011824 | None:intergenic | 15.0% |
| !!! | TAAGATTATGATTTTAACAT+AGG | - | chr1.4:14011209-14011228 | MS.gene32342:intron | 15.0% |
| !!! | TTTTGGTTTTTTTTTTTCAA+CGG | - | chr1.4:14011778-14011797 | MS.gene32342:intron | 15.0% |
| !! | ACCGAAAAATAATTAAATTG+TGG | + | chr1.4:14011753-14011772 | None:intergenic | 20.0% |
| !! | CAATATAGTCTAGATTTATT+AGG | + | chr1.4:14011652-14011671 | None:intergenic | 20.0% |
| !! | CATTTGATACAATATAACTA+TGG | + | chr1.4:14011921-14011940 | None:intergenic | 20.0% |
| !! | CTAATAAATCTAGACTATAT+TGG | - | chr1.4:14011650-14011669 | MS.gene32342:intron | 20.0% |
| !! | TCAAGAAAAATAACACAATT+AGG | + | chr1.4:14011306-14011325 | None:intergenic | 20.0% |
| !!! | AATTGTTCTTAAAATTTTGC+AGG | - | chr1.4:14011978-14011997 | MS.gene32342:CDS | 20.0% |
| !!! | AATTTTAATTATTAGCCATG+CGG | + | chr1.4:14011962-14011981 | None:intergenic | 20.0% |
| !!! | GCCACAATTTAATTATTTTT+CGG | - | chr1.4:14011749-14011768 | MS.gene32342:intron | 20.0% |
| ! | ATACAATATAACTATGGAGT+GGG | + | chr1.4:14011915-14011934 | None:intergenic | 25.0% |
| ! | TGATATAGTTCGATTCAAAA+AGG | - | chr1.4:14011828-14011847 | MS.gene32342:intron | 25.0% |
| ! | TGTTTGTGAATTGTTAATCA+TGG | + | chr1.4:14011701-14011720 | None:intergenic | 25.0% |
| ! | TTGTATCAAATGTAGTTGTA+TGG | - | chr1.4:14011929-14011948 | MS.gene32342:intron | 25.0% |
| AAAAACTTACCATCACTTGT+GGG | + | chr1.4:14011187-14011206 | None:intergenic | 30.0% | |
| AAAAAGAGTTTCTCAACATG+AGG | + | chr1.4:14011415-14011434 | None:intergenic | 30.0% | |
| AATCCATGAACCATATAAGA+TGG | + | chr1.4:14011278-14011297 | None:intergenic | 30.0% | |
| GATACAATATAACTATGGAG+TGG | + | chr1.4:14011916-14011935 | None:intergenic | 30.0% | |
| TAAAAACTTACCATCACTTG+TGG | + | chr1.4:14011188-14011207 | None:intergenic | 30.0% | |
| TACAGTTCAACTTTCAGATA+TGG | - | chr1.4:14011851-14011870 | MS.gene32342:intron | 30.0% | |
| !! | TTATTTTTCGGTGTGACTTT+TGG | - | chr1.4:14011761-14011780 | MS.gene32342:intron | 30.0% |
| AAGATGCGTAAATAAGACCA+AGG | + | chr1.4:14011114-14011133 | None:intergenic | 35.0% | |
| AATAGCATATGTCCTAATGC+TGG | - | chr1.4:14012019-14012038 | MS.gene32342:CDS | 35.0% | |
| AATCATGGTTCCATTCTCAT+CGG | + | chr1.4:14011686-14011705 | None:intergenic | 35.0% | |
| AATGATGTAAGTGAGAAGCA+AGG | + | chr1.4:14012048-14012067 | None:intergenic | 35.0% | |
| GAATCACAGCTATACATGAT+TGG | + | chr1.4:14011556-14011575 | None:intergenic | 35.0% | |
| GCAATTTAGACTACAACATC+TGG | - | chr1.4:14011484-14011503 | MS.gene32342:intron | 35.0% | |
| GTTGATCTCACCATCTTATA+TGG | - | chr1.4:14011265-14011284 | MS.gene32342:intron | 35.0% | |
| TCACCATCTTATATGGTTCA+TGG | - | chr1.4:14011272-14011291 | MS.gene32342:intron | 35.0% | |
| TTAGGACATATGCTATTGCT+TGG | + | chr1.4:14012016-14012035 | None:intergenic | 35.0% | |
| CTTCAACCAGAATCACATTG+TGG | + | chr1.4:14011528-14011547 | None:intergenic | 40.0% | |
| TTGTATGGTTTCAAACCGCA+TGG | - | chr1.4:14011944-14011963 | MS.gene32342:intron | 40.0% | |
| ! | ATATGGATGCCTTTTGTAGG+AGG | - | chr1.4:14011868-14011887 | MS.gene32342:intron | 40.0% |
| ! | CAGATATGGATGCCTTTTGT+AGG | - | chr1.4:14011865-14011884 | MS.gene32342:intron | 40.0% |
| ! | TATGGATGCCTTTTGTAGGA+GGG | - | chr1.4:14011869-14011888 | MS.gene32342:intron | 40.0% |
| ! | TTACGCATCTTTTGCGTCAA+TGG | - | chr1.4:14011123-14011142 | MS.gene32342:CDS | 40.0% |
| AGCATATGTCCTAATGCTGG+TGG | - | chr1.4:14012022-14012041 | MS.gene32342:CDS | 45.0% | |
| CAGTGACCACAATGTGATTC+TGG | - | chr1.4:14011519-14011538 | MS.gene32342:intron | 45.0% | |
| GATGGTGAGATCAACATCGA+AGG | + | chr1.4:14011260-14011279 | None:intergenic | 45.0% | |
| TTCTTCACAGTCTGTTGCTC+CGG | - | chr1.4:14011147-14011166 | MS.gene32342:intron | 45.0% | |
| ! | ATGGATGCCTTTTGTAGGAG+GGG | - | chr1.4:14011870-14011889 | MS.gene32342:intron | 45.0% |
| ! | GTTTCATGCACCGATGAGAA+TGG | - | chr1.4:14011673-14011692 | MS.gene32342:intron | 45.0% |
| TTACCATCACTTGTGGGAGC+AGG | + | chr1.4:14011181-14011200 | None:intergenic | 50.0% | |
| !! | TTTTGGGTGGTCGTGTTCCT+TGG | - | chr1.4:14011094-14011113 | MS.gene32342:CDS | 50.0% |
| GAAGCAAGGCCACCAGCATT+AGG | + | chr1.4:14012034-14012053 | None:intergenic | 55.0% | |
| GTGAGCTCCCCTCCTACAAA+AGG | + | chr1.4:14011880-14011899 | None:intergenic | 55.0% | |
| GCTCCTGCTCCCACAAGTGA+TGG | - | chr1.4:14011175-14011194 | MS.gene32342:intron | 60.0% | |
| TCACTTGTGGGAGCAGGAGC+AGG | + | chr1.4:14011175-14011194 | None:intergenic | 60.0% | |
| GTGGGAGCAGGAGCAGGAGC+CGG | + | chr1.4:14011169-14011188 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 14011082 | 14012073 | 14011082 | ID=MS.gene32342 |
| chr1.4 | mRNA | 14011082 | 14012073 | 14011082 | ID=MS.gene32342.t1;Parent=MS.gene32342 |
| chr1.4 | exon | 14011959 | 14012073 | 14011959 | ID=MS.gene32342.t1.exon1;Parent=MS.gene32342.t1 |
| chr1.4 | CDS | 14011959 | 14012073 | 14011959 | ID=cds.MS.gene32342.t1;Parent=MS.gene32342.t1 |
| chr1.4 | exon | 14011082 | 14011155 | 14011082 | ID=MS.gene32342.t1.exon2;Parent=MS.gene32342.t1 |
| chr1.4 | CDS | 14011082 | 14011155 | 14011082 | ID=cds.MS.gene32342.t1;Parent=MS.gene32342.t1 |
| Gene Sequence |
| Protein sequence |
