Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33113.t1 | XP_003588857.1 | 98.3 | 409 | 6 | 1 | 1 | 408 | 1 | 409 | 3.20E-227 | 797.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33113.t1 | Q8RXN4 | 70.8 | 391 | 108 | 2 | 20 | 407 | 21 | 408 | 4.5e-161 | 568.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33113.t1 | G7I876 | 98.3 | 409 | 6 | 1 | 1 | 408 | 1 | 409 | 2.3e-227 | 797.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051489 | MS.gene33113 | 0.95716 | 5.30E-115 | -1.69E-46 |
| MS.gene058482 | MS.gene33113 | 0.99671 | 3.77E-231 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene33113 | MS.gene022542 | PPI |
| MS.gene33113 | MS.gene62781 | PPI |
| MS.gene05932 | MS.gene33113 | PPI |
| MS.gene33113 | MS.gene61573 | PPI |
| MS.gene33113 | MS.gene049135 | PPI |
| MS.gene33113 | MS.gene04775 | PPI |
| MS.gene33113 | MS.gene015737 | PPI |
| MS.gene000261 | MS.gene33113 | PPI |
| MS.gene33113 | MS.gene020199 | PPI |
| MS.gene33113 | MS.gene39279 | PPI |
| MS.gene33113 | MS.gene019194 | PPI |
| MS.gene33113 | MS.gene022768 | PPI |
| MS.gene33113 | MS.gene69510 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33113.t1 | MTR_1g014040 | 98.289 | 409 | 6 | 1 | 1 | 408 | 1 | 409 | 0.0 | 828 |
| MS.gene33113.t1 | MTR_8g005990 | 65.940 | 367 | 125 | 0 | 42 | 408 | 35 | 401 | 0.0 | 534 |
| MS.gene33113.t1 | MTR_8g005990 | 61.853 | 367 | 117 | 1 | 42 | 408 | 35 | 378 | 8.41e-173 | 488 |
| MS.gene33113.t1 | MTR_8g010580 | 46.286 | 350 | 188 | 0 | 42 | 391 | 9 | 358 | 1.15e-105 | 317 |
| MS.gene33113.t1 | MTR_8g010580 | 46.037 | 328 | 177 | 0 | 42 | 369 | 9 | 336 | 8.23e-98 | 296 |
| MS.gene33113.t1 | MTR_3g046330 | 41.257 | 366 | 206 | 3 | 41 | 403 | 9 | 368 | 2.52e-97 | 296 |
| MS.gene33113.t1 | MTR_8g010580 | 45.541 | 314 | 171 | 0 | 42 | 355 | 9 | 322 | 8.20e-92 | 280 |
| MS.gene33113.t1 | MTR_8g103840 | 31.793 | 368 | 220 | 12 | 46 | 390 | 46 | 405 | 3.21e-48 | 169 |
| MS.gene33113.t1 | MTR_8g103840 | 31.436 | 369 | 224 | 13 | 46 | 390 | 5 | 368 | 4.17e-48 | 168 |
| MS.gene33113.t1 | MTR_8g103840 | 32.753 | 287 | 169 | 10 | 123 | 390 | 10 | 291 | 7.19e-38 | 139 |
| MS.gene33113.t1 | MTR_8g103840 | 31.511 | 311 | 184 | 12 | 102 | 390 | 4 | 307 | 1.82e-37 | 138 |
| MS.gene33113.t1 | MTR_8g103840 | 32.707 | 266 | 151 | 11 | 146 | 390 | 1 | 259 | 1.71e-32 | 124 |
| MS.gene33113.t1 | MTR_4g100350 | 26.786 | 168 | 115 | 4 | 58 | 222 | 59 | 221 | 2.51e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33113.t1 | AT4G31810 | 70.663 | 392 | 109 | 2 | 20 | 408 | 21 | 409 | 0.0 | 588 |
| MS.gene33113.t1 | AT3G60510 | 60.490 | 367 | 145 | 0 | 39 | 405 | 33 | 399 | 6.85e-162 | 461 |
| MS.gene33113.t1 | AT3G60510 | 56.633 | 392 | 145 | 1 | 39 | 405 | 33 | 424 | 1.72e-156 | 448 |
| MS.gene33113.t1 | AT3G60510 | 59.155 | 284 | 116 | 0 | 39 | 322 | 33 | 316 | 1.79e-118 | 347 |
| MS.gene33113.t1 | AT2G30660 | 46.110 | 347 | 187 | 0 | 41 | 387 | 3 | 349 | 7.32e-118 | 348 |
| MS.gene33113.t1 | AT5G65940 | 45.179 | 363 | 198 | 1 | 38 | 400 | 4 | 365 | 7.35e-116 | 343 |
| MS.gene33113.t1 | AT5G65940 | 45.179 | 363 | 198 | 1 | 38 | 400 | 4 | 365 | 7.35e-116 | 343 |
| MS.gene33113.t1 | AT2G30650 | 44.068 | 354 | 198 | 0 | 41 | 394 | 3 | 356 | 1.49e-111 | 332 |
| MS.gene33113.t1 | AT2G30660 | 45.741 | 317 | 172 | 0 | 71 | 387 | 1 | 317 | 4.83e-107 | 319 |
| MS.gene33113.t1 | AT5G65940 | 45.482 | 332 | 181 | 0 | 38 | 369 | 4 | 335 | 5.56e-107 | 319 |
| MS.gene33113.t1 | AT5G65940 | 44.410 | 322 | 179 | 0 | 38 | 359 | 4 | 325 | 5.17e-101 | 303 |
| MS.gene33113.t1 | AT2G30650 | 43.260 | 319 | 181 | 0 | 41 | 359 | 3 | 321 | 1.82e-96 | 292 |
| MS.gene33113.t1 | AT1G06550 | 38.764 | 356 | 216 | 1 | 45 | 400 | 13 | 366 | 3.78e-95 | 290 |
| MS.gene33113.t1 | AT3G24360 | 32.178 | 404 | 234 | 16 | 7 | 390 | 22 | 405 | 1.02e-51 | 179 |
| MS.gene33113.t1 | AT4G13360 | 32.486 | 354 | 213 | 13 | 58 | 390 | 64 | 412 | 2.34e-51 | 178 |
| MS.gene33113.t1 | AT3G24360 | 31.852 | 405 | 238 | 16 | 7 | 390 | 22 | 409 | 3.03e-50 | 175 |
| MS.gene33113.t1 | AT3G24360 | 34.146 | 287 | 165 | 11 | 123 | 390 | 3 | 284 | 1.87e-40 | 145 |
| MS.gene33113.t1 | AT3G24360 | 33.798 | 287 | 166 | 11 | 123 | 390 | 24 | 305 | 6.12e-40 | 145 |
| MS.gene33113.t1 | AT3G24360 | 33.681 | 288 | 167 | 11 | 122 | 390 | 25 | 307 | 7.49e-40 | 145 |
| MS.gene33113.t1 | AT3G24360 | 31.290 | 310 | 188 | 12 | 7 | 305 | 22 | 317 | 2.39e-33 | 128 |
Find 75 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTATGCAGGTTTCTATTT+TGG | 0.188063 | 1.1:-7439566 | MS.gene33113:intron |
| TGAGAAAAGTGACCTGGTTT+TGG | 0.242748 | 1.1:+7443691 | None:intergenic |
| TCATGACGTTGAGCGTAATT+TGG | 0.268085 | 1.1:+7443729 | None:intergenic |
| AGAGGCAGCGCTTTCTGTTC+TGG | 0.296350 | 1.1:-7442673 | MS.gene33113:CDS |
| TGGGAAGAAAACTCTGATAT+TGG | 0.296562 | 1.1:-7443211 | MS.gene33113:CDS |
| GGGGAATACCTGGCTCTTAC+AGG | 0.305410 | 1.1:-7437576 | MS.gene33113:CDS |
| AGGACAATGGGCTGTGGATC+TGG | 0.324205 | 1.1:-7439538 | MS.gene33113:CDS |
| GGATCTGGAATTTCTCTTCC+AGG | 0.324653 | 1.1:-7439523 | MS.gene33113:CDS |
| GTTCTGGTGCAGATGTTGTT+AGG | 0.338188 | 1.1:-7442657 | MS.gene33113:CDS |
| CTTACAGGAGATAAGCTTAA+TGG | 0.339083 | 1.1:-7437561 | MS.gene33113:CDS |
| AGGTAAGAAGCTCCGGCATC+AGG | 0.341189 | 1.1:+7439316 | None:intergenic |
| TGGAAGCCTATTTGAGCCTC+TGG | 0.348064 | 1.1:+7439340 | None:intergenic |
| TCTCACCCAGAGGCTCAAAT+AGG | 0.350016 | 1.1:-7439346 | MS.gene33113:CDS |
| GCTGTAGGCAGCACCAGTTC+TGG | 0.350396 | 1.1:+7435456 | None:intergenic |
| GTCACCACACGGAACATTCC+TGG | 0.373614 | 1.1:+7439505 | None:intergenic |
| GAAATCAGAGGAAACATTCT+TGG | 0.376274 | 1.1:+7435783 | None:intergenic |
| CGAGATGAGAAGCGAGTTTG+TGG | 0.378651 | 1.1:+7443789 | None:intergenic |
| TGAAGAACGCCTCGGTAAAC+TGG | 0.387307 | 1.1:-7437380 | MS.gene33113:CDS |
| GGAGGAAATCGTTGAAGCTT+TGG | 0.393441 | 1.1:-7436955 | MS.gene33113:intron |
| CGTCTAACGGAGAGAAATAA+TGG | 0.402607 | 1.1:+7435487 | None:intergenic |
| AAATAACTTTCAAACTCAAT+GGG | 0.412538 | 1.1:+7435979 | None:intergenic |
| AAAATAACTTTCAAACTCAA+TGG | 0.414771 | 1.1:+7435978 | None:intergenic |
| GAACGCGCTTACTACTTCAA+TGG | 0.424093 | 1.1:-7443514 | MS.gene33113:intron |
| TATTCATTTGTATATCTTCA+AGG | 0.428412 | 1.1:-7440953 | MS.gene33113:CDS |
| CGCGTGCAGCCATTCTCAAC+AGG | 0.429934 | 1.1:-7443548 | MS.gene33113:CDS |
| GAGGCATCTCTATCACAATA+TGG | 0.433121 | 1.1:-7437334 | MS.gene33113:CDS |
| GCATGCCAGTTTCACATGTA+AGG | 0.448906 | 1.1:+7435423 | None:intergenic |
| TGAAGAGGCTTTACGATTCG+TGG | 0.459797 | 1.1:-7443231 | MS.gene33113:CDS |
| TCTCGTCTACCCGGCTACTT+AGG | 0.467008 | 1.1:-7439289 | MS.gene33113:intron |
| ATTTACATTACCTAAGTAGC+CGG | 0.471536 | 1.1:+7439279 | None:intergenic |
| AGACAAAAGGTAAGAAGCTC+CGG | 0.474659 | 1.1:+7439309 | None:intergenic |
| TTGCAATACAGGGGAATACC+TGG | 0.494948 | 1.1:-7437586 | MS.gene33113:intron |
| AGACGAACTTGAGCCAGAAC+TGG | 0.500042 | 1.1:-7435469 | MS.gene33113:CDS |
| ATTCATTTGTATATCTTCAA+GGG | 0.510160 | 1.1:-7440952 | MS.gene33113:CDS |
| GTAAAGGATGCTACTTCTGT+CGG | 0.514331 | 1.1:+7437297 | None:intergenic |
| AGTGGTGTTTGACAACTCTT+CGG | 0.514714 | 1.1:-7436019 | MS.gene33113:CDS |
| ATAGGCTTCCACCCTGATGC+CGG | 0.521366 | 1.1:-7439328 | MS.gene33113:CDS |
| TAAAGGATGCTACTTCTGTC+GGG | 0.527142 | 1.1:+7437298 | None:intergenic |
| AGAAGCTGGCCTGTTGAGAA+TGG | 0.532999 | 1.1:+7443539 | None:intergenic |
| CTGTATCACTCGCTCAATGA+AGG | 0.534281 | 1.1:-7442634 | MS.gene33113:intron |
| GCTTATCTCCTGTAAGAGCC+AGG | 0.536076 | 1.1:+7437568 | None:intergenic |
| TGATGCAACATTACTTGTAA+AGG | 0.536607 | 1.1:+7437281 | None:intergenic |
| TGTAGGTTGCTAGGTTGAAG+AGG | 0.539313 | 1.1:-7443246 | MS.gene33113:intron |
| GCAGGGTGTTCGAGCACGAA+TGG | 0.548551 | 1.1:-7435675 | MS.gene33113:intron |
| AGAGATGCCTCTACAACAGA+TGG | 0.556087 | 1.1:+7437346 | None:intergenic |
| GGAAGCCTATTTGAGCCTCT+GGG | 0.558406 | 1.1:+7439341 | None:intergenic |
| GGTAAGAAGCTCCGGCATCA+GGG | 0.562896 | 1.1:+7439317 | None:intergenic |
| TTTACATTACCTAAGTAGCC+GGG | 0.564814 | 1.1:+7439280 | None:intergenic |
| GATGGAAGGACAATGGGCTG+TGG | 0.566644 | 1.1:-7439544 | MS.gene33113:CDS |
| TAAGGTTCTCGCAATGCTGT+AGG | 0.569057 | 1.1:+7435441 | None:intergenic |
| ATTCTTGGAAATTGTCTGAA+CGG | 0.569846 | 1.1:+7435798 | None:intergenic |
| GTAAGCGCGTTCAAAGAAGC+TGG | 0.578878 | 1.1:+7443525 | None:intergenic |
| AGCCGGGTAGACGAGACAAA+AGG | 0.582060 | 1.1:+7439296 | None:intergenic |
| GAAGAGGCTTTACGATTCGT+GGG | 0.584667 | 1.1:-7443230 | MS.gene33113:CDS |
| AAAAGATGTATCAGAGGACA+TGG | 0.585915 | 1.1:-7435514 | MS.gene33113:CDS |
| AACTTTCAAACTCAATGGGG+AGG | 0.587496 | 1.1:+7435983 | None:intergenic |
| TTAGTGAATAATGTGTAGCA+AGG | 0.603652 | 1.1:+7437517 | None:intergenic |
| GCTTGGCTTGAAGAACGCCT+CGG | 0.607099 | 1.1:-7437388 | MS.gene33113:CDS |
| GCTATTCTTTCAGGAGAAGG+AGG | 0.609266 | 1.1:-7436060 | MS.gene33113:intron |
| AGACGATCCATCTGTTGTAG+AGG | 0.616126 | 1.1:-7437353 | MS.gene33113:CDS |
| TGTTTCCTCTGATTTCGTTG+AGG | 0.617126 | 1.1:-7435776 | MS.gene33113:intron |
| TCTGGCTCAAGTTCGTCTAA+CGG | 0.617927 | 1.1:+7435474 | None:intergenic |
| AGAAGAAGATTCCCAAAACC+AGG | 0.620014 | 1.1:-7443703 | MS.gene33113:intron |
| AAATTGTCTGAACGGATACA+CGG | 0.628555 | 1.1:+7435806 | None:intergenic |
| CATTACCTCAACGAAATCAG+AGG | 0.628804 | 1.1:+7435771 | None:intergenic |
| CAGCTGAGTCTTCAGATGAG+TGG | 0.631379 | 1.1:-7436037 | MS.gene33113:CDS |
| AACTTTAAAAGATGTATCAG+AGG | 0.645633 | 1.1:-7435520 | MS.gene33113:CDS |
| TAATGAATTTAGATACGGGA+AGG | 0.651153 | 1.1:-7435868 | MS.gene33113:intron |
| ATGTTTCAGTCATGATACAG+TGG | 0.668884 | 1.1:-7436976 | MS.gene33113:CDS |
| TTTCAGTCATGATACAGTGG+AGG | 0.670723 | 1.1:-7436973 | MS.gene33113:CDS |
| TCTTCCAGGAATGTTCCGTG+TGG | 0.686395 | 1.1:-7439509 | MS.gene33113:CDS |
| AATAACTTTCAAACTCAATG+GGG | 0.690229 | 1.1:+7435980 | None:intergenic |
| AAGAAGCTCCGGCATCAGGG+TGG | 0.692971 | 1.1:+7439320 | None:intergenic |
| GGTAGAGCAGAGAAAGAACG+AGG | 0.719204 | 1.1:+7443750 | None:intergenic |
| CGTCTGTGACCAGTTTACCG+AGG | 0.739947 | 1.1:+7437371 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 7435434 | 7443835 | 7435434 | ID=MS.gene33113 |
| chr1.1 | mRNA | 7435434 | 7443835 | 7435434 | ID=MS.gene33113.t1;Parent=MS.gene33113 |
| chr1.1 | exon | 7443704 | 7443835 | 7443704 | ID=MS.gene33113.t1.exon1;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7443704 | 7443835 | 7443704 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7443515 | 7443595 | 7443515 | ID=MS.gene33113.t1.exon2;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7443515 | 7443595 | 7443515 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7443195 | 7443263 | 7443195 | ID=MS.gene33113.t1.exon3;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7443195 | 7443263 | 7443195 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7442635 | 7442698 | 7442635 | ID=MS.gene33113.t1.exon4;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7442635 | 7442698 | 7442635 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7440934 | 7441013 | 7440934 | ID=MS.gene33113.t1.exon5;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7440934 | 7441013 | 7440934 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7439495 | 7439578 | 7439495 | ID=MS.gene33113.t1.exon6;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7439495 | 7439578 | 7439495 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7439290 | 7439374 | 7439290 | ID=MS.gene33113.t1.exon7;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7439290 | 7439374 | 7439290 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7437512 | 7437597 | 7437512 | ID=MS.gene33113.t1.exon8;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7437512 | 7437597 | 7437512 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7437295 | 7437416 | 7437295 | ID=MS.gene33113.t1.exon9;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7437295 | 7437416 | 7437295 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7436956 | 7437016 | 7436956 | ID=MS.gene33113.t1.exon10;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7436956 | 7437016 | 7436956 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7435971 | 7436069 | 7435971 | ID=MS.gene33113.t1.exon11;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7435971 | 7436069 | 7435971 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7435777 | 7435878 | 7435777 | ID=MS.gene33113.t1.exon12;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7435777 | 7435878 | 7435777 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7435652 | 7435693 | 7435652 | ID=MS.gene33113.t1.exon13;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7435652 | 7435693 | 7435652 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| chr1.1 | exon | 7435434 | 7435553 | 7435434 | ID=MS.gene33113.t1.exon14;Parent=MS.gene33113.t1 |
| chr1.1 | CDS | 7435434 | 7435553 | 7435434 | ID=cds.MS.gene33113.t1;Parent=MS.gene33113.t1 |
| Gene Sequence |
| Protein sequence |
