Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020199.t1 | KEH18076.1 | 97.9 | 341 | 7 | 0 | 1 | 341 | 1 | 341 | 5.50E-188 | 666.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020199.t1 | Q9LKJ1 | 69.5 | 328 | 100 | 0 | 9 | 336 | 8 | 335 | 1.8e-134 | 480.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020199.t1 | A0A072TLH4 | 97.9 | 341 | 7 | 0 | 1 | 341 | 1 | 341 | 3.9e-188 | 666.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene020199 | MS.gene022542 | PPI |
| MS.gene020199 | MS.gene62781 | PPI |
| MS.gene05932 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene049135 | PPI |
| MS.gene051489 | MS.gene020199 | PPI |
| MS.gene015787 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene049084 | PPI |
| MS.gene000261 | MS.gene020199 | PPI |
| MS.gene022768 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene015737 | PPI |
| MS.gene39279 | MS.gene020199 | PPI |
| MS.gene69510 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene04775 | PPI |
| MS.gene016092 | MS.gene020199 | PPI |
| MS.gene61573 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene05958 | PPI |
| MS.gene33113 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene39279 | PPI |
| MS.gene019194 | MS.gene020199 | PPI |
| MS.gene020199 | MS.gene022768 | PPI |
| MS.gene05880 | MS.gene020199 | PPI |
| MS.gene058482 | MS.gene020199 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020199.t1 | MTR_8g010580 | 97.947 | 341 | 7 | 0 | 1 | 341 | 1 | 341 | 0.0 | 695 |
| MS.gene020199.t1 | MTR_8g010580 | 98.512 | 336 | 5 | 0 | 1 | 336 | 1 | 336 | 0.0 | 686 |
| MS.gene020199.t1 | MTR_8g010580 | 98.447 | 322 | 5 | 0 | 1 | 322 | 1 | 322 | 0.0 | 659 |
| MS.gene020199.t1 | MTR_8g005990 | 46.061 | 330 | 178 | 0 | 7 | 336 | 33 | 362 | 3.75e-103 | 309 |
| MS.gene020199.t1 | MTR_1g014040 | 46.037 | 328 | 177 | 0 | 9 | 336 | 42 | 369 | 4.23e-103 | 309 |
| MS.gene020199.t1 | MTR_3g046330 | 43.917 | 337 | 186 | 2 | 1 | 336 | 1 | 335 | 1.23e-92 | 281 |
| MS.gene020199.t1 | MTR_8g005990 | 43.939 | 330 | 162 | 1 | 7 | 336 | 33 | 339 | 8.08e-92 | 279 |
| MS.gene020199.t1 | MTR_8g103840 | 35.736 | 333 | 190 | 10 | 22 | 336 | 58 | 384 | 1.03e-52 | 179 |
| MS.gene020199.t1 | MTR_8g103840 | 35.030 | 334 | 195 | 10 | 22 | 336 | 17 | 347 | 1.56e-51 | 175 |
| MS.gene020199.t1 | MTR_8g103840 | 35.496 | 262 | 149 | 8 | 92 | 336 | 12 | 270 | 1.17e-40 | 144 |
| MS.gene020199.t1 | MTR_8g103840 | 35.496 | 262 | 149 | 8 | 92 | 336 | 28 | 286 | 1.63e-40 | 144 |
| MS.gene020199.t1 | MTR_8g103840 | 35.270 | 241 | 136 | 8 | 113 | 336 | 1 | 238 | 5.29e-35 | 129 |
| MS.gene020199.t1 | MTR_2027s0010 | 27.528 | 178 | 125 | 1 | 12 | 189 | 2 | 175 | 4.15e-15 | 74.3 |
| MS.gene020199.t1 | MTR_3g085700 | 31.461 | 178 | 114 | 3 | 17 | 188 | 16 | 191 | 1.67e-14 | 73.2 |
| MS.gene020199.t1 | MTR_4g122220 | 29.947 | 187 | 122 | 4 | 12 | 196 | 7 | 186 | 4.75e-14 | 71.6 |
| MS.gene020199.t1 | MTR_4g100350 | 25.698 | 179 | 125 | 4 | 23 | 198 | 57 | 230 | 1.98e-12 | 67.4 |
| MS.gene020199.t1 | MTR_8g064990 | 28.671 | 143 | 96 | 3 | 23 | 163 | 81 | 219 | 6.93e-11 | 63.2 |
| MS.gene020199.t1 | MTR_8g064990 | 28.671 | 143 | 96 | 3 | 23 | 163 | 7 | 145 | 9.70e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene020199.t1 | AT5G65940 | 69.069 | 333 | 102 | 1 | 9 | 340 | 8 | 340 | 1.11e-180 | 503 |
| MS.gene020199.t1 | AT5G65940 | 69.512 | 328 | 100 | 0 | 9 | 336 | 8 | 335 | 1.55e-179 | 502 |
| MS.gene020199.t1 | AT5G65940 | 69.512 | 328 | 100 | 0 | 9 | 336 | 8 | 335 | 1.55e-179 | 502 |
| MS.gene020199.t1 | AT5G65940 | 68.224 | 321 | 102 | 0 | 9 | 329 | 8 | 328 | 6.85e-171 | 478 |
| MS.gene020199.t1 | AT2G30660 | 67.073 | 328 | 108 | 0 | 9 | 336 | 4 | 331 | 7.98e-171 | 480 |
| MS.gene020199.t1 | AT2G30650 | 65.644 | 326 | 112 | 0 | 11 | 336 | 6 | 331 | 4.69e-160 | 452 |
| MS.gene020199.t1 | AT2G30660 | 66.221 | 299 | 101 | 0 | 38 | 336 | 1 | 299 | 7.65e-154 | 436 |
| MS.gene020199.t1 | AT2G30650 | 63.354 | 322 | 118 | 0 | 8 | 329 | 3 | 324 | 1.73e-150 | 426 |
| MS.gene020199.t1 | AT4G31810 | 44.817 | 328 | 181 | 0 | 9 | 336 | 40 | 367 | 8.07e-100 | 301 |
| MS.gene020199.t1 | AT3G60510 | 48.466 | 326 | 168 | 0 | 11 | 336 | 38 | 363 | 1.31e-97 | 295 |
| MS.gene020199.t1 | AT1G06550 | 44.545 | 330 | 181 | 1 | 7 | 336 | 8 | 335 | 1.56e-94 | 286 |
| MS.gene020199.t1 | AT3G60510 | 45.014 | 351 | 168 | 1 | 11 | 336 | 38 | 388 | 5.04e-92 | 281 |
| MS.gene020199.t1 | AT3G60510 | 47.653 | 277 | 145 | 0 | 11 | 287 | 38 | 314 | 5.86e-81 | 249 |
| MS.gene020199.t1 | AT3G24360 | 36.119 | 335 | 186 | 12 | 22 | 336 | 58 | 384 | 8.64e-55 | 185 |
| MS.gene020199.t1 | AT4G13360 | 36.716 | 335 | 188 | 11 | 22 | 336 | 61 | 391 | 1.42e-54 | 184 |
| MS.gene020199.t1 | AT3G24360 | 35.714 | 336 | 190 | 12 | 22 | 336 | 58 | 388 | 1.52e-52 | 179 |
| MS.gene020199.t1 | AT3G24360 | 38.224 | 259 | 150 | 9 | 22 | 271 | 58 | 315 | 1.78e-42 | 150 |
| MS.gene020199.t1 | AT3G24360 | 35.985 | 264 | 145 | 10 | 92 | 336 | 5 | 263 | 3.05e-41 | 146 |
| MS.gene020199.t1 | AT3G24360 | 35.985 | 264 | 145 | 10 | 92 | 336 | 26 | 284 | 3.69e-41 | 146 |
| MS.gene020199.t1 | AT3G24360 | 35.985 | 264 | 145 | 10 | 92 | 336 | 28 | 286 | 3.95e-41 | 146 |
| MS.gene020199.t1 | AT4G16210 | 29.747 | 158 | 103 | 3 | 7 | 163 | 5 | 155 | 4.71e-14 | 71.6 |
| MS.gene020199.t1 | AT5G43280 | 27.624 | 181 | 120 | 4 | 18 | 188 | 18 | 197 | 9.24e-12 | 64.3 |
| MS.gene020199.t1 | AT5G43280 | 27.624 | 181 | 120 | 4 | 18 | 188 | 18 | 197 | 2.34e-11 | 63.9 |
Find 70 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAGAGCATTCAATTGTTT+TGG | 0.250822 | 8.4:+78053221 | None:intergenic |
| TTGCAGGTTTCAATTCTTAA+TGG | 0.257914 | 8.4:-78049839 | MS.gene020199:intron |
| GGAGAATATGTTGGTCTTAC+AGG | 0.259688 | 8.4:-78048844 | MS.gene020199:CDS |
| AAATAATCCCAAAGCTGTTT+CGG | 0.303784 | 8.4:+78049352 | None:intergenic |
| GTCAATAATTGCAGATACTA+TGG | 0.328804 | 8.4:+78047075 | None:intergenic |
| TGCTATGCCCGAAACAGCTT+TGG | 0.328892 | 8.4:-78049360 | MS.gene020199:CDS |
| AATAATCCCAAAGCTGTTTC+GGG | 0.332057 | 8.4:+78049353 | None:intergenic |
| ATTAGACAAGGCAGGCTCTT+AGG | 0.333895 | 8.4:-78046174 | MS.gene020199:CDS |
| TAGTTATGGTTTCCCACCTT+TGG | 0.349718 | 8.4:+78045954 | None:intergenic |
| ATATAAAATGAATTATTTGA+TGG | 0.359065 | 8.4:-78049976 | MS.gene020199:CDS |
| CCTTGCTATGGTGTCCTTGT+AGG | 0.366990 | 8.4:+78046106 | None:intergenic |
| AACTAATTTATGTCTAGTTA+TGG | 0.369368 | 8.4:+78045940 | None:intergenic |
| TTTCATATGGTATAGGTGAT+TGG | 0.369959 | 8.4:-78050028 | MS.gene020199:intron |
| GGTATAGGTGATTGGAGATT+TGG | 0.392868 | 8.4:-78050020 | MS.gene020199:intron |
| TGTTAAATTGATTGTCCTAA+AGG | 0.399260 | 8.4:-78051774 | MS.gene020199:intron |
| TGCACAGAAATTATCGTCAT+TGG | 0.403375 | 8.4:-78047134 | MS.gene020199:intron |
| ATCGTCATGGGAGGTGGCGC+TGG | 0.407520 | 8.4:-78049815 | MS.gene020199:CDS |
| AACCTGATCTTCATCCAGTT+TGG | 0.407756 | 8.4:+78053425 | None:intergenic |
| TGTCGGAGATGCTTTCTTCA+AGG | 0.412605 | 8.4:+78046514 | None:intergenic |
| GGTTACATTTATCAGGGAAA+TGG | 0.414501 | 8.4:-78051072 | MS.gene020199:intron |
| TAATATGAGGCGCCTACATC+AGG | 0.422559 | 8.4:+78049329 | None:intergenic |
| TATTATTTGTCTAGACTAGC+TGG | 0.430192 | 8.4:-78049311 | MS.gene020199:CDS |
| AGGACAATCAATTTAACATC+AGG | 0.430392 | 8.4:+78051779 | None:intergenic |
| GCTATGCCCGAAACAGCTTT+GGG | 0.440444 | 8.4:-78049359 | MS.gene020199:CDS |
| CGCCAAACTGGATGAAGATC+AGG | 0.455421 | 8.4:-78053427 | MS.gene020199:intron |
| AGAGCAGCCTTCCCTAAAAC+AGG | 0.460836 | 8.4:-78047044 | MS.gene020199:CDS |
| TGATCTTCATCCAGTTTGGC+GGG | 0.473292 | 8.4:+78053429 | None:intergenic |
| ATCTTCATCCAGTTTGGCGG+GGG | 0.486411 | 8.4:+78053431 | None:intergenic |
| GGAGACTGAAGCTATGAGTA+AGG | 0.498581 | 8.4:-78046567 | MS.gene020199:CDS |
| CCATAGCAAGGACTTCTTCG+AGG | 0.507020 | 8.4:-78046094 | MS.gene020199:intron |
| AGTATGTAGCATACCGATGA+AGG | 0.512916 | 8.4:+78048766 | None:intergenic |
| CTTACAGGTGCTAGGTTGGA+TGG | 0.513409 | 8.4:-78048829 | MS.gene020199:CDS |
| CTGATCTTCATCCAGTTTGG+CGG | 0.518696 | 8.4:+78053428 | None:intergenic |
| AATGGAATCGTCATGGGAGG+TGG | 0.522285 | 8.4:-78049821 | MS.gene020199:CDS |
| AATTCTTAATGGAATCGTCA+TGG | 0.523281 | 8.4:-78049828 | MS.gene020199:CDS |
| TTGGGATTATTTCCTGATGT+AGG | 0.525045 | 8.4:-78049341 | MS.gene020199:CDS |
| ATAATTGCAGATACTATGGC+TGG | 0.526109 | 8.4:+78047079 | None:intergenic |
| GATCTTCATCCAGTTTGGCG+GGG | 0.533241 | 8.4:+78053430 | None:intergenic |
| ATTCTTAATGGAATCGTCAT+GGG | 0.537888 | 8.4:-78049827 | MS.gene020199:CDS |
| TAGTCTAGACAAATAATATG+AGG | 0.540571 | 8.4:+78049316 | None:intergenic |
| CTATACTGATAGATAAAGAC+AGG | 0.546864 | 8.4:-78045980 | MS.gene020199:CDS |
| GGCGCTGGTGCTTCTATGCA+TGG | 0.548075 | 8.4:-78049800 | MS.gene020199:CDS |
| AGAAATATTTCACGATGATG+AGG | 0.552047 | 8.4:-78051807 | MS.gene020199:CDS |
| AACAGGATAGTGTTTATCAC+AGG | 0.554120 | 8.4:-78047027 | MS.gene020199:intron |
| CTATGAGTAAGGCAGATAGT+TGG | 0.555340 | 8.4:-78046556 | MS.gene020199:CDS |
| CAATTCTATACTCGTAAACA+AGG | 0.558815 | 8.4:+78046139 | None:intergenic |
| TGGTCTTACAGGTGCTAGGT+TGG | 0.559352 | 8.4:-78048833 | MS.gene020199:CDS |
| CAAATTGCAGGAGAATATGT+TGG | 0.561540 | 8.4:-78048853 | MS.gene020199:intron |
| CAAGGCAGGCTCTTAGGTGT+TGG | 0.562954 | 8.4:-78046168 | MS.gene020199:CDS |
| TAATCAATAAGTGTTTCTCT+AGG | 0.569952 | 8.4:-78046734 | MS.gene020199:CDS |
| GGCAACATACAGTAAGCCTC+AGG | 0.571282 | 8.4:-78049955 | MS.gene020199:intron |
| CCTCGAAGAAGTCCTTGCTA+TGG | 0.585985 | 8.4:+78046094 | None:intergenic |
| ACATTTATCAGGGAAATGGA+AGG | 0.587193 | 8.4:-78051068 | MS.gene020199:intron |
| ATGTTGGTCTTACAGGTGCT+AGG | 0.591347 | 8.4:-78048837 | MS.gene020199:CDS |
| GAAATATTTCACGATGATGA+GGG | 0.593731 | 8.4:-78051806 | MS.gene020199:CDS |
| CATTTATCAGGGAAATGGAA+GGG | 0.594293 | 8.4:-78051067 | MS.gene020199:intron |
| ATTGTTTGTCATATCCTACA+AGG | 0.594529 | 8.4:-78046120 | MS.gene020199:CDS |
| TAAAGACAGGAAACCAAAGG+TGG | 0.600690 | 8.4:-78045967 | MS.gene020199:CDS |
| GGTGCAGAAATGCTTGCTTG+TGG | 0.601628 | 8.4:-78048808 | MS.gene020199:CDS |
| GTATTGTGCAAAGGTTCTGG+TGG | 0.605879 | 8.4:-78053282 | MS.gene020199:intron |
| ATTGGAAGAATCATTATGCA+AGG | 0.625818 | 8.4:-78047116 | MS.gene020199:CDS |
| GTATGTAGCATACCGATGAA+GGG | 0.626747 | 8.4:+78048767 | None:intergenic |
| GTTGCTAATGACGCAAGAGG+AGG | 0.628706 | 8.4:-78051015 | MS.gene020199:intron |
| AGATAAAGACAGGAAACCAA+AGG | 0.644265 | 8.4:-78045970 | MS.gene020199:CDS |
| GCTGTTGCTAATGACGCAAG+AGG | 0.648895 | 8.4:-78051018 | MS.gene020199:CDS |
| AAAGACAGGAAACCAAAGGT+GGG | 0.655182 | 8.4:-78045966 | MS.gene020199:CDS |
| CTTAATGGAATCGTCATGGG+AGG | 0.671508 | 8.4:-78049824 | MS.gene020199:CDS |
| GGAAGAATCATTATGCAAGG+TGG | 0.673416 | 8.4:-78047113 | MS.gene020199:CDS |
| CCTACAAGGACACCATAGCA+AGG | 0.674917 | 8.4:-78046106 | MS.gene020199:CDS |
| GTGTTTCTCTAGGAAAACAG+TGG | 0.677969 | 8.4:-78046724 | MS.gene020199:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 78045953 | 78053460 | 78045953 | ID=MS.gene020199 |
| chr8.4 | mRNA | 78045953 | 78053460 | 78045953 | ID=MS.gene020199.t1;Parent=MS.gene020199 |
| chr8.4 | exon | 78053428 | 78053460 | 78053428 | ID=MS.gene020199.t1.exon1;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78053428 | 78053460 | 78053428 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78053211 | 78053291 | 78053211 | ID=MS.gene020199.t1.exon2;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78053211 | 78053291 | 78053211 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78051775 | 78051843 | 78051775 | ID=MS.gene020199.t1.exon3;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78051775 | 78051843 | 78051775 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78051016 | 78051079 | 78051016 | ID=MS.gene020199.t1.exon4;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78051016 | 78051079 | 78051016 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78049956 | 78050035 | 78049956 | ID=MS.gene020199.t1.exon5;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78049956 | 78050035 | 78049956 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78049772 | 78049855 | 78049772 | ID=MS.gene020199.t1.exon6;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78049772 | 78049855 | 78049772 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78049303 | 78049387 | 78049303 | ID=MS.gene020199.t1.exon7;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78049303 | 78049387 | 78049303 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78048780 | 78048865 | 78048780 | ID=MS.gene020199.t1.exon8;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78048780 | 78048865 | 78048780 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78047028 | 78047149 | 78047028 | ID=MS.gene020199.t1.exon9;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78047028 | 78047149 | 78047028 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78046704 | 78046764 | 78046704 | ID=MS.gene020199.t1.exon10;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78046704 | 78046764 | 78046704 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78046490 | 78046588 | 78046490 | ID=MS.gene020199.t1.exon11;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78046490 | 78046588 | 78046490 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78046095 | 78046196 | 78046095 | ID=MS.gene020199.t1.exon12;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78046095 | 78046196 | 78046095 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| chr8.4 | exon | 78045953 | 78046012 | 78045953 | ID=MS.gene020199.t1.exon13;Parent=MS.gene020199.t1 |
| chr8.4 | CDS | 78045953 | 78046012 | 78045953 | ID=cds.MS.gene020199.t1;Parent=MS.gene020199.t1 |
| Gene Sequence |
| Protein sequence |
