Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34389.t1 | XP_013469751.1 | 96.2 | 186 | 5 | 2 | 1 | 184 | 1 | 186 | 9.50E-86 | 326.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34389.t1 | P11432 | 74.0 | 196 | 39 | 1 | 1 | 184 | 1 | 196 | 1.3e-70 | 267.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34389.t1 | A0A072VP35 | 96.2 | 186 | 5 | 2 | 1 | 184 | 1 | 186 | 6.8e-86 | 326.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene34389 | 0.822099 | 2.84E-53 | -1.69E-46 |
| MS.gene049369 | MS.gene34389 | 0.816538 | 5.25E-52 | -1.69E-46 |
| MS.gene05081 | MS.gene34389 | 0.809472 | 1.86E-50 | -1.69E-46 |
| MS.gene051734 | MS.gene34389 | 0.8441 | 9.32E-59 | -1.69E-46 |
| MS.gene051838 | MS.gene34389 | 0.805321 | 1.41E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene34389 | 0.826153 | 3.17E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene34389 | 0.807406 | 5.13E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene34389 | 0.806783 | 6.96E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene34389 | 0.833242 | 5.97E-56 | -1.69E-46 |
| MS.gene052870 | MS.gene34389 | 0.843107 | 1.72E-58 | -1.69E-46 |
| MS.gene052872 | MS.gene34389 | 0.839411 | 1.61E-57 | -1.69E-46 |
| MS.gene053449 | MS.gene34389 | 0.82108 | 4.88E-53 | -1.69E-46 |
| MS.gene053824 | MS.gene34389 | 0.812926 | 3.32E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene34389 | 0.826754 | 2.28E-54 | -1.69E-46 |
| MS.gene055224 | MS.gene34389 | 0.818795 | 1.63E-52 | -1.69E-46 |
| MS.gene055539 | MS.gene34389 | 0.835829 | 1.34E-56 | -1.69E-46 |
| MS.gene055741 | MS.gene34389 | 0.813323 | 2.71E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene34389 | 0.815781 | 7.75E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene34389 | 0.814114 | 1.82E-51 | -1.69E-46 |
| MS.gene057927 | MS.gene34389 | 0.810119 | 1.35E-50 | -1.69E-46 |
| MS.gene058120 | MS.gene34389 | 0.804823 | 1.80E-49 | -1.69E-46 |
| MS.gene059674 | MS.gene34389 | 0.813631 | 2.32E-51 | -1.69E-46 |
| MS.gene059717 | MS.gene34389 | 0.831841 | 1.33E-55 | -1.69E-46 |
| MS.gene059788 | MS.gene34389 | 0.807845 | 4.14E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene34389 | 0.812766 | 3.59E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene34389 | 0.801007 | 1.10E-48 | -1.69E-46 |
| MS.gene060981 | MS.gene34389 | 0.80659 | 7.64E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene34389 | 0.821062 | 4.93E-53 | -1.69E-46 |
| MS.gene061231 | MS.gene34389 | 0.81164 | 6.33E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34389.t1 | MTR_1g102830 | 96.237 | 186 | 5 | 2 | 1 | 184 | 1 | 186 | 8.73e-126 | 352 |
| MS.gene34389.t1 | MTR_1g102810 | 93.491 | 169 | 10 | 1 | 17 | 184 | 14 | 182 | 5.72e-113 | 319 |
| MS.gene34389.t1 | MTR_1g102780 | 81.111 | 180 | 19 | 1 | 20 | 184 | 21 | 200 | 1.04e-98 | 284 |
| MS.gene34389.t1 | MTR_1g102570 | 79.775 | 178 | 23 | 1 | 20 | 184 | 21 | 198 | 3.27e-98 | 282 |
| MS.gene34389.t1 | MTR_1g102800 | 80.203 | 197 | 26 | 1 | 1 | 184 | 1 | 197 | 5.28e-98 | 282 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34389.t1 | AT3G22840 | 50.000 | 192 | 77 | 3 | 7 | 184 | 9 | 195 | 3.47e-58 | 181 |
| MS.gene34389.t1 | AT4G14690 | 50.521 | 192 | 74 | 4 | 11 | 184 | 5 | 193 | 1.19e-53 | 169 |
Find 46 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTTCCATTGATTCTTTC+AGG | 0.165068 | 1.2:-76522099 | None:intergenic |
| TAGAAGGGATGTTGTTAAAT+TGG | 0.226805 | 1.2:-76521553 | None:intergenic |
| GTTGTTGACAGTTGCTTCTT+TGG | 0.263072 | 1.2:+76522230 | MS.gene34389:CDS |
| ACACTAACACCTTCAAATAA+TGG | 0.346554 | 1.2:-76522255 | None:intergenic |
| ATTCCAAAGCTCTGCATTTG+AGG | 0.355412 | 1.2:-76522304 | None:intergenic |
| GGTTTGTTCGATCAAATATC+TGG | 0.369233 | 1.2:+76522174 | MS.gene34389:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.370659 | 1.2:+76522338 | MS.gene34389:CDS |
| TAGTTTGAAAGTTCGATCTA+TGG | 0.378474 | 1.2:+76521604 | MS.gene34389:CDS |
| AAGCTAGGCATATACATAGA+AGG | 0.387722 | 1.2:-76521569 | None:intergenic |
| GGCTGTTACAGAATATGTTA+AGG | 0.394991 | 1.2:+76522359 | MS.gene34389:CDS |
| TCTTTGGTTCCATTATTTGA+AGG | 0.404737 | 1.2:+76522246 | MS.gene34389:CDS |
| AATGGAAGGTTAGCCATGAT+TGG | 0.409893 | 1.2:+76522111 | MS.gene34389:CDS |
| GCTGTTACAGAATATGTTAA+GGG | 0.415066 | 1.2:+76522360 | MS.gene34389:CDS |
| CGGATTTGATGGCATTTGGT+GGG | 0.428133 | 1.2:+76522070 | MS.gene34389:CDS |
| GCAGGCTGAGGAGTCACCTT+TGG | 0.441538 | 1.2:-76521874 | None:intergenic |
| TCTAGGCCTGCTTGAAATGC+TGG | 0.450968 | 1.2:-76521525 | None:intergenic |
| TTGTTAAATTGGTTAACTCT+AGG | 0.463045 | 1.2:-76521542 | None:intergenic |
| TGTCCTCAAATGCAGAGCTT+TGG | 0.467503 | 1.2:+76522301 | MS.gene34389:CDS |
| AGTGTTGAATCTAAGTCAAA+AGG | 0.480583 | 1.2:+76522273 | MS.gene34389:CDS |
| TCGGATTTGATGGCATTTGG+TGG | 0.487791 | 1.2:+76522069 | MS.gene34389:CDS |
| TGCAGATACAAAACCAATCA+TGG | 0.488652 | 1.2:-76522124 | None:intergenic |
| GAATGGAAGACTTGCTATGT+TGG | 0.490221 | 1.2:+76522323 | MS.gene34389:CDS |
| AATGGAAGACTTGCTATGTT+GGG | 0.502682 | 1.2:+76522324 | MS.gene34389:CDS |
| TTCGATCAAATATCTGGTGG+TGG | 0.522060 | 1.2:+76522180 | MS.gene34389:CDS |
| CTAGCATTCCTTCTGAAGCT+AGG | 0.539206 | 1.2:-76521584 | None:intergenic |
| CCTGCTTGAAATGCTGGTCA+TGG | 0.548863 | 1.2:-76521519 | None:intergenic |
| GCAGCGCCTGAAAGAATCAA+TGG | 0.552540 | 1.2:+76522093 | MS.gene34389:CDS |
| CACTTGTATACAAGAACCAT+GGG | 0.556587 | 1.2:-76522206 | None:intergenic |
| GGATTTGATGGCATTTGGTG+GGG | 0.562192 | 1.2:+76522071 | MS.gene34389:CDS |
| AGCTAGGCATATACATAGAA+GGG | 0.567045 | 1.2:-76521568 | None:intergenic |
| GAACGAGTAGAAGCAGGCTG+AGG | 0.574059 | 1.2:-76521886 | None:intergenic |
| TCAAATGCAGAGCTTTGGAA+TGG | 0.580798 | 1.2:+76522306 | MS.gene34389:CDS |
| TTTGGTGAACGAGTAGAAGC+AGG | 0.581281 | 1.2:-76521892 | None:intergenic |
| TGCTTCTACTCGTTCACCAA+AGG | 0.583895 | 1.2:+76521894 | MS.gene34389:CDS |
| ATAAAACTCTACTTAAACAA+GGG | 0.585278 | 1.2:-76522388 | None:intergenic |
| GGTGGAATTAGCAAAAGGGC+AGG | 0.590541 | 1.2:+76522152 | MS.gene34389:CDS |
| GAAAGTTCGATCTATGGCTG+AGG | 0.590785 | 1.2:+76521610 | MS.gene34389:CDS |
| TTGTTCGATCAAATATCTGG+TGG | 0.594040 | 1.2:+76522177 | MS.gene34389:CDS |
| TGTATATGCCTAGCTTCAGA+AGG | 0.603350 | 1.2:+76521576 | MS.gene34389:CDS |
| CCATGACCAGCATTTCAAGC+AGG | 0.603962 | 1.2:+76521519 | MS.gene34389:CDS |
| GTGGAATTAGCAAAAGGGCA+GGG | 0.608172 | 1.2:+76522153 | MS.gene34389:CDS |
| TATAAAACTCTACTTAAACA+AGG | 0.611188 | 1.2:-76522389 | None:intergenic |
| ACACTTGTATACAAGAACCA+TGG | 0.618963 | 1.2:-76522207 | None:intergenic |
| CGCCTGAAAGAATCAATGGA+AGG | 0.624472 | 1.2:+76522097 | MS.gene34389:CDS |
| AGGGCAGAAAGAGCAACCAA+AGG | 0.630670 | 1.2:+76521858 | MS.gene34389:CDS |
| TATCTGGTGGTGGAATCCCA+TGG | 0.688884 | 1.2:+76522190 | MS.gene34389:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTTCTTTACATTATTC+AGG | + | chr1.2:76521834-76521853 | MS.gene34389:intron | 20.0% |
| !! | ATCATCATTATTCATCAAAA+AGG | - | chr1.2:76522021-76522040 | None:intergenic | 20.0% |
| !! | GGTTTATCAAACAAATATTA+AGG | + | chr1.2:76521640-76521659 | MS.gene34389:intron | 20.0% |
| !! | TTTATTATGATGTTTACCTT+TGG | - | chr1.2:76521913-76521932 | None:intergenic | 20.0% |
| ! | AGTATAGTCATGTAAAGTAT+TGG | + | chr1.2:76521974-76521993 | MS.gene34389:intron | 25.0% |
| ! | TTCTTTACATTATTCAGGAA+GGG | + | chr1.2:76521839-76521858 | MS.gene34389:intron | 25.0% |
| ! | TTGTTAAATTGGTTAACTCT+AGG | - | chr1.2:76521545-76521564 | None:intergenic | 25.0% |
| ! | TTTCTTTACATTATTCAGGA+AGG | + | chr1.2:76521838-76521857 | MS.gene34389:intron | 25.0% |
| !!! | GTAGTTGATTTTGTAAATTC+CGG | + | chr1.2:76521772-76521791 | MS.gene34389:intron | 25.0% |
| ACACTAACACCTTCAAATAA+TGG | - | chr1.2:76522258-76522277 | None:intergenic | 30.0% | |
| GCTGTTACAGAATATGTTAA+GGG | + | chr1.2:76522360-76522379 | MS.gene34389:CDS | 30.0% | |
| TAGAAGGGATGTTGTTAAAT+TGG | - | chr1.2:76521556-76521575 | None:intergenic | 30.0% | |
| TAGTTTGAAAGTTCGATCTA+TGG | + | chr1.2:76521604-76521623 | MS.gene34389:CDS | 30.0% | |
| TTACATGACTATACTCTTCT+TGG | - | chr1.2:76521969-76521988 | None:intergenic | 30.0% | |
| ! | AGTGTTGAATCTAAGTCAAA+AGG | + | chr1.2:76522273-76522292 | MS.gene34389:CDS | 30.0% |
| ! | CACAAAATTTTCGGATTTGA+TGG | + | chr1.2:76522059-76522078 | MS.gene34389:CDS | 30.0% |
| ! | GCAGATAAGCACAAAATTTT+CGG | + | chr1.2:76522050-76522069 | MS.gene34389:intron | 30.0% |
| ! | TCTTTGGTTCCATTATTTGA+AGG | + | chr1.2:76522246-76522265 | MS.gene34389:CDS | 30.0% |
| !!! | ATTGGTTTTGTATCTGCAAT+GGG | + | chr1.2:76522129-76522148 | MS.gene34389:CDS | 30.0% |
| !!! | GTCATGTAAAGTATTGGTTT+TGG | + | chr1.2:76521980-76521999 | MS.gene34389:intron | 30.0% |
| AAGCTAGGCATATACATAGA+AGG | - | chr1.2:76521572-76521591 | None:intergenic | 35.0% | |
| AAGTGACATTCAAGACGTTA+TGG | - | chr1.2:76521803-76521822 | None:intergenic | 35.0% | |
| ACACTTGTATACAAGAACCA+TGG | - | chr1.2:76522210-76522229 | None:intergenic | 35.0% | |
| AGCTAGGCATATACATAGAA+GGG | - | chr1.2:76521571-76521590 | None:intergenic | 35.0% | |
| ATCTATGGCTGAGGTAATTA+AGG | + | chr1.2:76521619-76521638 | MS.gene34389:intron | 35.0% | |
| CACTTGTATACAAGAACCAT+GGG | - | chr1.2:76522209-76522228 | None:intergenic | 35.0% | |
| GGCTGTTACAGAATATGTTA+AGG | + | chr1.2:76522359-76522378 | MS.gene34389:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.2:76522338-76522357 | MS.gene34389:CDS | 35.0% | |
| TGCAGATACAAAACCAATCA+TGG | - | chr1.2:76522127-76522146 | None:intergenic | 35.0% | |
| TTGTTCGATCAAATATCTGG+TGG | + | chr1.2:76522177-76522196 | MS.gene34389:CDS | 35.0% | |
| ! | ACTCTTCTTGGACACAATTT+TGG | - | chr1.2:76521957-76521976 | None:intergenic | 35.0% |
| ! | GGTTTGTTCGATCAAATATC+TGG | + | chr1.2:76522174-76522193 | MS.gene34389:CDS | 35.0% |
| !! | AACCTTCCATTGATTCTTTC+AGG | - | chr1.2:76522102-76522121 | None:intergenic | 35.0% |
| !! | AATGGAAGACTTGCTATGTT+GGG | + | chr1.2:76522324-76522343 | MS.gene34389:CDS | 35.0% |
| !! | TTTTCGGATTTGATGGCATT+TGG | + | chr1.2:76522066-76522085 | MS.gene34389:CDS | 35.0% |
| !!! | GATTGGTTTTGTATCTGCAA+TGG | + | chr1.2:76522128-76522147 | MS.gene34389:CDS | 35.0% |
| !!! | TTGGTTTTGTATCTGCAATG+GGG | + | chr1.2:76522130-76522149 | MS.gene34389:CDS | 35.0% |
| ATTCCAAAGCTCTGCATTTG+AGG | - | chr1.2:76522307-76522326 | None:intergenic | 40.0% | |
| GTTGTTGACAGTTGCTTCTT+TGG | + | chr1.2:76522230-76522249 | MS.gene34389:CDS | 40.0% | |
| TCAAGACGTTATGGATTCTC+CGG | - | chr1.2:76521794-76521813 | None:intergenic | 40.0% | |
| TGTATATGCCTAGCTTCAGA+AGG | + | chr1.2:76521576-76521595 | MS.gene34389:CDS | 40.0% | |
| TTCGATCAAATATCTGGTGG+TGG | + | chr1.2:76522180-76522199 | MS.gene34389:CDS | 40.0% | |
| ! | AATGGAAGGTTAGCCATGAT+TGG | + | chr1.2:76522111-76522130 | MS.gene34389:CDS | 40.0% |
| !! | GAATGGAAGACTTGCTATGT+TGG | + | chr1.2:76522323-76522342 | MS.gene34389:CDS | 40.0% |
| !! | TCAAATGCAGAGCTTTGGAA+TGG | + | chr1.2:76522306-76522325 | MS.gene34389:CDS | 40.0% |
| !!! | TGGTTTTGTATCTGCAATGG+GGG | + | chr1.2:76522131-76522150 | MS.gene34389:CDS | 40.0% |
| ATGGGGGTGGAATTAGCAAA+AGG | + | chr1.2:76522147-76522166 | MS.gene34389:CDS | 45.0% | |
| CGCCTGAAAGAATCAATGGA+AGG | + | chr1.2:76522097-76522116 | MS.gene34389:CDS | 45.0% | |
| GAAAGTTCGATCTATGGCTG+AGG | + | chr1.2:76521610-76521629 | MS.gene34389:CDS | 45.0% | |
| GTGGAATTAGCAAAAGGGCA+GGG | + | chr1.2:76522153-76522172 | MS.gene34389:CDS | 45.0% | |
| TGCTTCTACTCGTTCACCAA+AGG | + | chr1.2:76521894-76521913 | MS.gene34389:CDS | 45.0% | |
| TGGGGGTGGAATTAGCAAAA+GGG | + | chr1.2:76522148-76522167 | MS.gene34389:CDS | 45.0% | |
| TGTCCTCAAATGCAGAGCTT+TGG | + | chr1.2:76522301-76522320 | MS.gene34389:CDS | 45.0% | |
| TTTGGTGAACGAGTAGAAGC+AGG | - | chr1.2:76521895-76521914 | None:intergenic | 45.0% | |
| ! | CGGATTTGATGGCATTTGGT+GGG | + | chr1.2:76522070-76522089 | MS.gene34389:CDS | 45.0% |
| ! | GGATTTGATGGCATTTGGTG+GGG | + | chr1.2:76522071-76522090 | MS.gene34389:CDS | 45.0% |
| ! | TCGGATTTGATGGCATTTGG+TGG | + | chr1.2:76522069-76522088 | MS.gene34389:CDS | 45.0% |
| ! | TTTTGTATCTGCAATGGGGG+TGG | + | chr1.2:76522134-76522153 | MS.gene34389:CDS | 45.0% |
| !! | CTAGCATTCCTTCTGAAGCT+AGG | - | chr1.2:76521587-76521606 | None:intergenic | 45.0% |
| AGGGCAGAAAGAGCAACCAA+AGG | + | chr1.2:76521858-76521877 | MS.gene34389:CDS | 50.0% | |
| CCATGACCAGCATTTCAAGC+AGG | + | chr1.2:76521519-76521538 | MS.gene34389:CDS | 50.0% | |
| CCTGCTTGAAATGCTGGTCA+TGG | - | chr1.2:76521522-76521541 | None:intergenic | 50.0% | |
| GCAGCGCCTGAAAGAATCAA+TGG | + | chr1.2:76522093-76522112 | MS.gene34389:CDS | 50.0% | |
| GGTGGAATTAGCAAAAGGGC+AGG | + | chr1.2:76522152-76522171 | MS.gene34389:CDS | 50.0% | |
| TCTAGGCCTGCTTGAAATGC+TGG | - | chr1.2:76521528-76521547 | None:intergenic | 50.0% | |
| !! | TATCTGGTGGTGGAATCCCA+TGG | + | chr1.2:76522190-76522209 | MS.gene34389:CDS | 50.0% |
| GAACGAGTAGAAGCAGGCTG+AGG | - | chr1.2:76521889-76521908 | None:intergenic | 55.0% | |
| GCAGGCTGAGGAGTCACCTT+TGG | - | chr1.2:76521877-76521896 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 76521482 | 76522398 | 76521482 | ID=MS.gene34389 |
| chr1.2 | mRNA | 76521482 | 76522398 | 76521482 | ID=MS.gene34389.t1;Parent=MS.gene34389 |
| chr1.2 | exon | 76521482 | 76521631 | 76521482 | ID=MS.gene34389.t1.exon1;Parent=MS.gene34389.t1 |
| chr1.2 | CDS | 76521482 | 76521631 | 76521482 | ID=cds.MS.gene34389.t1;Parent=MS.gene34389.t1 |
| chr1.2 | exon | 76521856 | 76521915 | 76521856 | ID=MS.gene34389.t1.exon2;Parent=MS.gene34389.t1 |
| chr1.2 | CDS | 76521856 | 76521915 | 76521856 | ID=cds.MS.gene34389.t1;Parent=MS.gene34389.t1 |
| chr1.2 | exon | 76522054 | 76522398 | 76522054 | ID=MS.gene34389.t1.exon3;Parent=MS.gene34389.t1 |
| chr1.2 | CDS | 76522054 | 76522398 | 76522054 | ID=cds.MS.gene34389.t1;Parent=MS.gene34389.t1 |
| Gene Sequence |
| Protein sequence |
