Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35459.t1 | ADD71138.1 | 98.2 | 224 | 4 | 0 | 1 | 224 | 89 | 312 | 3.80E-121 | 444.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35459.t1 | Q9SJE8 | 52.2 | 226 | 100 | 2 | 1 | 221 | 69 | 291 | 4.8e-64 | 245.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35459.t1 | D4NZK1 | 98.2 | 224 | 4 | 0 | 1 | 224 | 89 | 312 | 2.7e-121 | 444.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene35459 | 0.81977 | 9.76E-53 | -1.69E-46 |
| MS.gene051385 | MS.gene35459 | 0.810944 | 8.96E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene35459 | 0.821767 | 3.39E-53 | -1.69E-46 |
| MS.gene05240 | MS.gene35459 | 0.805343 | 1.40E-49 | -1.69E-46 |
| MS.gene053403 | MS.gene35459 | 0.80572 | 1.16E-49 | -1.69E-46 |
| MS.gene053406 | MS.gene35459 | 0.839139 | 1.89E-57 | -1.69E-46 |
| MS.gene054120 | MS.gene35459 | 0.842067 | 3.24E-58 | -1.69E-46 |
| MS.gene054421 | MS.gene35459 | 0.825212 | 5.30E-54 | -1.69E-46 |
| MS.gene054742 | MS.gene35459 | 0.822335 | 2.50E-53 | -1.69E-46 |
| MS.gene055240 | MS.gene35459 | 0.808692 | 2.73E-50 | -1.69E-46 |
| MS.gene055595 | MS.gene35459 | 0.818417 | 1.98E-52 | -1.69E-46 |
| MS.gene055597 | MS.gene35459 | 0.808307 | 3.30E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene35459 | 0.81922 | 1.30E-52 | -1.69E-46 |
| MS.gene056920 | MS.gene35459 | 0.82092 | 5.32E-53 | -1.69E-46 |
| MS.gene056923 | MS.gene35459 | 0.800713 | 1.27E-48 | -1.69E-46 |
| MS.gene060087 | MS.gene35459 | 0.827315 | 1.67E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATAATTGAACCTATACTTCT+TGG | 0.239685 | 2.1:+14890456 | MS.gene35459:CDS |
| GATATTGTTGCCAAGGATAT+TGG | 0.241933 | 2.1:-14891488 | None:intergenic |
| AGTTGTTAAGGAAGCCATTA+AGG | 0.268610 | 2.1:-14891557 | None:intergenic |
| TATCGGATTGATGCCCTTAA+TGG | 0.282687 | 2.1:+14891543 | MS.gene35459:CDS |
| TTGGCTGGTGTTCTTGGACT+TGG | 0.319754 | 2.1:+14890393 | MS.gene35459:CDS |
| ATGAAGGCTAGATATGATTA+TGG | 0.325434 | 2.1:+14890639 | MS.gene35459:CDS |
| GATAAGCACATTGCCTTGAT+AGG | 0.359354 | 2.1:-14891524 | None:intergenic |
| CTTGGAAAAGGTTTAAATAG+AGG | 0.383071 | 2.1:+14890357 | MS.gene35459:CDS |
| TCATATCTAGCCTTCATTAG+AGG | 0.384146 | 2.1:-14890633 | None:intergenic |
| TTCTTGTGCCCTGTTTGGGC+TGG | 0.387615 | 2.1:+14890795 | MS.gene35459:CDS |
| TATCAAGGCAATGTGCTTAT+CGG | 0.393258 | 2.1:+14891526 | MS.gene35459:CDS |
| TGGCAACAATATCAAAAGAT+TGG | 0.402570 | 2.1:+14891498 | MS.gene35459:CDS |
| GTACCTTAGTGAAGTTGTTA+AGG | 0.410620 | 2.1:-14891569 | None:intergenic |
| TAATTGACACTGCACAAGAT+AGG | 0.418272 | 2.1:+14890724 | MS.gene35459:CDS |
| TTGTGCCCTGTTTGGGCTGG+TGG | 0.433334 | 2.1:+14890798 | MS.gene35459:CDS |
| ATTATGATTCCAAGAAGTAT+AGG | 0.433596 | 2.1:-14890465 | None:intergenic |
| AGATCACCACCAGCCCAAAC+AGG | 0.433992 | 2.1:-14890804 | None:intergenic |
| GGCTAGATATGATTATGGTT+TGG | 0.440433 | 2.1:+14890644 | MS.gene35459:CDS |
| GAATCTGAAACGACCATGGC+AGG | 0.446207 | 2.1:-14891466 | None:intergenic |
| GTTGTTAAGGAAGCCATTAA+GGG | 0.460812 | 2.1:-14891556 | None:intergenic |
| GTTTCAGATTCCAATATCCT+TGG | 0.467661 | 2.1:+14891478 | MS.gene35459:CDS |
| TTTGAATTATTTCAGCTGCA+GGG | 0.486846 | 2.1:+14890589 | MS.gene35459:intron |
| TCGATTTATACCTCTAATGA+AGG | 0.491939 | 2.1:+14890623 | MS.gene35459:CDS |
| GTGCAGTGTCAATTATCTCA+TGG | 0.511026 | 2.1:-14890716 | None:intergenic |
| GCAGGAGCAACACTTGGAAA+AGG | 0.511844 | 2.1:+14890345 | None:intergenic |
| TGGACTTGGATCTTATTACA+TGG | 0.514673 | 2.1:+14890407 | MS.gene35459:CDS |
| TGGTGGATTAATTTCTGTGT+TGG | 0.515117 | 2.1:+14890764 | MS.gene35459:CDS |
| GTAACAACAATTCTAGTTGG+TGG | 0.515822 | 2.1:+14890747 | MS.gene35459:CDS |
| CTTCCTTAACAACTTCACTA+AGG | 0.516652 | 2.1:+14891566 | MS.gene35459:CDS |
| AAAAGATTGGAAACCTATCA+AGG | 0.530845 | 2.1:+14891511 | MS.gene35459:CDS |
| AGGGTAACAACAATTCTAGT+TGG | 0.536338 | 2.1:+14890744 | MS.gene35459:CDS |
| AATTGACACTGCACAAGATA+GGG | 0.540293 | 2.1:+14890725 | MS.gene35459:CDS |
| AAAACAAGTCGAAGATAACT+TGG | 0.544545 | 2.1:+14891069 | MS.gene35459:CDS |
| TTGAATTATTTCAGCTGCAG+GGG | 0.563412 | 2.1:+14890590 | MS.gene35459:intron |
| GCACATGCAGGAGCAACACT+TGG | 0.568214 | 2.1:+14890339 | None:intergenic |
| GCAAGGTGGGAACCCTGCCA+TGG | 0.570019 | 2.1:+14891453 | MS.gene35459:CDS |
| TCAAATAAATCATTTATGCA+AGG | 0.574471 | 2.1:+14891025 | MS.gene35459:CDS |
| ATGGTTCATACAATTTCAAG+AGG | 0.574977 | 2.1:+14890426 | MS.gene35459:CDS |
| ATTGGAATCTGAAACGACCA+TGG | 0.657737 | 2.1:-14891470 | None:intergenic |
| AATCTGAAACGACCATGGCA+GGG | 0.657967 | 2.1:-14891465 | None:intergenic |
| GATCACCACCAGCCCAAACA+GGG | 0.679414 | 2.1:-14890803 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTACAATAACATAATTT+TGG | - | chr2.1:14891109-14891128 | None:intergenic | 10.0% |
| !!! | AATTTTGTAAATTGTGATTA+TGG | + | chr2.1:14890892-14890911 | MS.gene35459:intron | 15.0% |
| !! | ATATATTGAGAAATATTCCA+TGG | - | chr2.1:14891158-14891177 | None:intergenic | 20.0% |
| !! | ATTAATTCTTGGAAAATTGT+AGG | + | chr2.1:14890962-14890981 | MS.gene35459:intron | 20.0% |
| !! | TCAAAAAACATTGAAAAACT+TGG | + | chr2.1:14890840-14890859 | MS.gene35459:CDS | 20.0% |
| !! | TCAAATAAATCATTTATGCA+AGG | + | chr2.1:14891025-14891044 | MS.gene35459:CDS | 20.0% |
| !! | TGTTGAAATTGATTAATTCT+TGG | + | chr2.1:14890951-14890970 | MS.gene35459:intron | 20.0% |
| !!! | ATTAGTTTAATACCTTTTGT+AGG | + | chr2.1:14891422-14891441 | MS.gene35459:intron | 20.0% |
| !!! | TATTTGATAATTTCACTAGT+AGG | + | chr2.1:14890523-14890542 | MS.gene35459:intron | 20.0% |
| ! | ATAATTGAACCTATACTTCT+TGG | + | chr2.1:14890456-14890475 | MS.gene35459:CDS | 25.0% |
| ! | TCTACTACGATTTACATATA+CGG | + | chr2.1:14891342-14891361 | MS.gene35459:intron | 25.0% |
| !! | ATTATGATTCCAAGAAGTAT+AGG | - | chr2.1:14890468-14890487 | None:intergenic | 25.0% |
| !! | GTTTATCTTGACATTTTGTT+TGG | + | chr2.1:14890671-14890690 | MS.gene35459:CDS | 25.0% |
| AAAACAAGTCGAAGATAACT+TGG | + | chr2.1:14891069-14891088 | MS.gene35459:CDS | 30.0% | |
| AAAAGATTGGAAACCTATCA+AGG | + | chr2.1:14891511-14891530 | MS.gene35459:CDS | 30.0% | |
| ATGAAGGCTAGATATGATTA+TGG | + | chr2.1:14890639-14890658 | MS.gene35459:CDS | 30.0% | |
| ATGGTTCATACAATTTCAAG+AGG | + | chr2.1:14890426-14890445 | MS.gene35459:CDS | 30.0% | |
| CTACTACGATTTACATATAC+GGG | + | chr2.1:14891343-14891362 | MS.gene35459:intron | 30.0% | |
| CTTGGAAAAGGTTTAAATAG+AGG | + | chr2.1:14890357-14890376 | MS.gene35459:CDS | 30.0% | |
| TCAAATATGTTGAAAAGTCG+TGG | - | chr2.1:14891314-14891333 | None:intergenic | 30.0% | |
| TCATCAAATCAAAATGTCCA+TGG | + | chr2.1:14891138-14891157 | MS.gene35459:intron | 30.0% | |
| TCGATTTATACCTCTAATGA+AGG | + | chr2.1:14890623-14890642 | MS.gene35459:CDS | 30.0% | |
| TGGCAACAATATCAAAAGAT+TGG | + | chr2.1:14891498-14891517 | MS.gene35459:CDS | 30.0% | |
| TTGCAAAATTGACCTACAAA+AGG | - | chr2.1:14891437-14891456 | None:intergenic | 30.0% | |
| TTTGAATTATTTCAGCTGCA+GGG | + | chr2.1:14890589-14890608 | MS.gene35459:intron | 30.0% | |
| ! | AAACTTGGCAACTTTTTAGA+AGG | + | chr2.1:14890855-14890874 | MS.gene35459:CDS | 30.0% |
| ! | ATCTTTTGATATTGTTGCCA+AGG | - | chr2.1:14891498-14891517 | None:intergenic | 30.0% |
| ! | TAGAGGTTTAGCAACATTTT+TGG | + | chr2.1:14890374-14890393 | MS.gene35459:CDS | 30.0% |
| ! | TCTTGGAAAATTGTAGGTTT+TGG | + | chr2.1:14890968-14890987 | MS.gene35459:intron | 30.0% |
| ! | TTTTGAATTATTTCAGCTGC+AGG | + | chr2.1:14890588-14890607 | MS.gene35459:intron | 30.0% |
| ! | TTTTGTAGGTCAATTTTGCA+AGG | + | chr2.1:14891436-14891455 | MS.gene35459:intron | 30.0% |
| !!! | GGTTTTGGAGATGAATATTT+TGG | + | chr2.1:14890983-14891002 | MS.gene35459:intron | 30.0% |
| AATTGACACTGCACAAGATA+GGG | + | chr2.1:14890725-14890744 | MS.gene35459:CDS | 35.0% | |
| AGACATGTCAATGTTCAACT+AGG | - | chr2.1:14891212-14891231 | None:intergenic | 35.0% | |
| AGGGTAACAACAATTCTAGT+TGG | + | chr2.1:14890744-14890763 | MS.gene35459:CDS | 35.0% | |
| AGTTGTTAAGGAAGCCATTA+AGG | - | chr2.1:14891560-14891579 | None:intergenic | 35.0% | |
| CTTCCTTAACAACTTCACTA+AGG | + | chr2.1:14891566-14891585 | MS.gene35459:CDS | 35.0% | |
| GATATTGTTGCCAAGGATAT+TGG | - | chr2.1:14891491-14891510 | None:intergenic | 35.0% | |
| GGCTAGATATGATTATGGTT+TGG | + | chr2.1:14890644-14890663 | MS.gene35459:CDS | 35.0% | |
| GTTGTTAAGGAAGCCATTAA+GGG | - | chr2.1:14891559-14891578 | None:intergenic | 35.0% | |
| GTTTCAGATTCCAATATCCT+TGG | + | chr2.1:14891478-14891497 | MS.gene35459:CDS | 35.0% | |
| TAAGTCAGAGTAGACAAAGA+CGG | - | chr2.1:14891399-14891418 | None:intergenic | 35.0% | |
| TAATTGACACTGCACAAGAT+AGG | + | chr2.1:14890724-14890743 | MS.gene35459:CDS | 35.0% | |
| TATCAAGGCAATGTGCTTAT+CGG | + | chr2.1:14891526-14891545 | MS.gene35459:CDS | 35.0% | |
| TGGACTTGGATCTTATTACA+TGG | + | chr2.1:14890407-14890426 | MS.gene35459:CDS | 35.0% | |
| TTGAATTATTTCAGCTGCAG+GGG | + | chr2.1:14890590-14890609 | MS.gene35459:intron | 35.0% | |
| ! | GTAACAACAATTCTAGTTGG+TGG | + | chr2.1:14890747-14890766 | MS.gene35459:CDS | 35.0% |
| ! | GTCTCCAATTTAGTTCATGT+GGG | + | chr2.1:14891228-14891247 | MS.gene35459:intron | 35.0% |
| ! | TCATATCTAGCCTTCATTAG+AGG | - | chr2.1:14890636-14890655 | None:intergenic | 35.0% |
| ! | TCTCCAATTTAGTTCATGTG+GGG | + | chr2.1:14891229-14891248 | MS.gene35459:intron | 35.0% |
| ! | TGTCTCCAATTTAGTTCATG+TGG | + | chr2.1:14891227-14891246 | MS.gene35459:intron | 35.0% |
| !! | TGGTGGATTAATTTCTGTGT+TGG | + | chr2.1:14890764-14890783 | MS.gene35459:CDS | 35.0% |
| !!! | TATTTTGGAACACTAGATGC+TGG | + | chr2.1:14890998-14891017 | MS.gene35459:CDS | 35.0% |
| AGTCCCCACATGAACTAAAT+TGG | - | chr2.1:14891235-14891254 | None:intergenic | 40.0% | |
| GATAAGCACATTGCCTTGAT+AGG | - | chr2.1:14891527-14891546 | None:intergenic | 40.0% | |
| GTGCAGTGTCAATTATCTCA+TGG | - | chr2.1:14890719-14890738 | None:intergenic | 40.0% | |
| TATCGGATTGATGCCCTTAA+TGG | + | chr2.1:14891543-14891562 | MS.gene35459:CDS | 40.0% | |
| ! | AAGTCGAAGATAACTTGGTG+AGG | + | chr2.1:14891074-14891093 | MS.gene35459:intron | 40.0% |
| ! | ACATTTTCTTGTGCCCTGTT+TGG | + | chr2.1:14890790-14890809 | MS.gene35459:CDS | 40.0% |
| ! | ATTGGAATCTGAAACGACCA+TGG | - | chr2.1:14891473-14891492 | None:intergenic | 40.0% |
| ! | CATTTTCTTGTGCCCTGTTT+GGG | + | chr2.1:14890791-14890810 | MS.gene35459:CDS | 40.0% |
| ! | GTAGGTCAATTTTGCAAGGT+GGG | + | chr2.1:14891440-14891459 | MS.gene35459:intron | 40.0% |
| ! | TGTAGGTCAATTTTGCAAGG+TGG | + | chr2.1:14891439-14891458 | MS.gene35459:intron | 40.0% |
| !!! | ACATTTTTGGCTGGTGTTCT+TGG | + | chr2.1:14890387-14890406 | MS.gene35459:CDS | 40.0% |
| !!! | GGTTTAGCAACATTTTTGGC+TGG | + | chr2.1:14890378-14890397 | MS.gene35459:CDS | 40.0% |
| ! | AATCTGAAACGACCATGGCA+GGG | - | chr2.1:14891468-14891487 | None:intergenic | 45.0% |
| !! | TTTTTAGAAGGTGCGCAAGC+AGG | + | chr2.1:14890867-14890886 | MS.gene35459:intron | 45.0% |
| !! | AAAAAAAATAAAAATTGTAT+TGG | - | chr2.1:14890570-14890589 | None:intergenic | 5.0% |
| TTGGCTGGTGTTCTTGGACT+TGG | + | chr2.1:14890393-14890412 | MS.gene35459:CDS | 50.0% | |
| ! | GAATCTGAAACGACCATGGC+AGG | - | chr2.1:14891469-14891488 | None:intergenic | 50.0% |
| AGATCACCACCAGCCCAAAC+AGG | - | chr2.1:14890807-14890826 | None:intergenic | 55.0% | |
| GATCACCACCAGCCCAAACA+GGG | - | chr2.1:14890806-14890825 | None:intergenic | 55.0% | |
| TTCTTGTGCCCTGTTTGGGC+TGG | + | chr2.1:14890795-14890814 | MS.gene35459:CDS | 55.0% | |
| ! | TTGTGCCCTGTTTGGGCTGG+TGG | + | chr2.1:14890798-14890817 | MS.gene35459:CDS | 60.0% |
| GCAAGGTGGGAACCCTGCCA+TGG | + | chr2.1:14891453-14891472 | MS.gene35459:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 14890349 | 14891587 | 14890349 | ID=MS.gene35459 |
| chr2.1 | mRNA | 14890349 | 14891587 | 14890349 | ID=MS.gene35459.t1;Parent=MS.gene35459 |
| chr2.1 | exon | 14890349 | 14890498 | 14890349 | ID=MS.gene35459.t1.exon1;Parent=MS.gene35459.t1 |
| chr2.1 | CDS | 14890349 | 14890498 | 14890349 | ID=cds.MS.gene35459.t1;Parent=MS.gene35459.t1 |
| chr2.1 | exon | 14890604 | 14890876 | 14890604 | ID=MS.gene35459.t1.exon2;Parent=MS.gene35459.t1 |
| chr2.1 | CDS | 14890604 | 14890876 | 14890604 | ID=cds.MS.gene35459.t1;Parent=MS.gene35459.t1 |
| chr2.1 | exon | 14890984 | 14891090 | 14890984 | ID=MS.gene35459.t1.exon3;Parent=MS.gene35459.t1 |
| chr2.1 | CDS | 14890984 | 14891090 | 14890984 | ID=cds.MS.gene35459.t1;Parent=MS.gene35459.t1 |
| chr2.1 | exon | 14891444 | 14891587 | 14891444 | ID=MS.gene35459.t1.exon4;Parent=MS.gene35459.t1 |
| chr2.1 | CDS | 14891444 | 14891587 | 14891444 | ID=cds.MS.gene35459.t1;Parent=MS.gene35459.t1 |
| Gene Sequence |
| Protein sequence |
