Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36051.t1 | XP_003628534.1 | 98.9 | 375 | 4 | 0 | 1 | 375 | 1 | 375 | 5.60E-210 | 740 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36051.t1 | Q8RWJ7 | 68.2 | 314 | 80 | 6 | 1 | 294 | 1 | 314 | 8.4e-114 | 411.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36051.t1 | G7LDQ2 | 98.9 | 375 | 4 | 0 | 1 | 375 | 1 | 375 | 4.0e-210 | 740.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene36051.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene36051 | -0.805054 | 1.61E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene36051 | -0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene050826 | MS.gene36051 | -0.827084 | 1.90E-54 | -1.69E-46 |
| MS.gene050953 | MS.gene36051 | -0.818733 | 1.68E-52 | -1.69E-46 |
| MS.gene052673 | MS.gene36051 | 0.822681 | 2.08E-53 | -1.69E-46 |
| MS.gene054291 | MS.gene36051 | 0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene054379 | MS.gene36051 | -0.805845 | 1.10E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene36051 | -0.810861 | 9.34E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene36051 | -0.80333 | 3.67E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene36051 | -0.813219 | 2.86E-51 | -1.69E-46 |
| MS.gene056480 | MS.gene36051 | -0.808144 | 3.58E-50 | -1.69E-46 |
| MS.gene056776 | MS.gene36051 | -0.804939 | 1.70E-49 | -1.69E-46 |
| MS.gene059952 | MS.gene36051 | -0.80454 | 2.06E-49 | -1.69E-46 |
| MS.gene060137 | MS.gene36051 | 0.803152 | 4.00E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene36051 | -0.81921 | 1.31E-52 | -1.69E-46 |
| MS.gene06052 | MS.gene36051 | -0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene060634 | MS.gene36051 | 0.827936 | 1.19E-54 | -1.69E-46 |
| MS.gene060802 | MS.gene36051 | -0.812341 | 4.45E-51 | -1.69E-46 |
| MS.gene060879 | MS.gene36051 | -0.800641 | 1.31E-48 | -1.69E-46 |
| MS.gene060932 | MS.gene36051 | -0.810284 | 1.24E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36051.t1 | MTR_8g061040 | 98.933 | 375 | 4 | 0 | 1 | 375 | 1 | 375 | 0.0 | 776 |
| MS.gene36051.t1 | MTR_3g110260 | 40.076 | 262 | 125 | 4 | 1 | 260 | 1 | 232 | 6.58e-65 | 206 |
| MS.gene36051.t1 | MTR_8g014075 | 38.372 | 258 | 148 | 3 | 1 | 258 | 1 | 247 | 1.06e-49 | 168 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36051.t1 | AT3G18380 | 68.153 | 314 | 80 | 6 | 1 | 294 | 1 | 314 | 2.12e-152 | 434 |
| MS.gene36051.t1 | AT3G18380 | 67.937 | 315 | 80 | 7 | 1 | 294 | 1 | 315 | 1.31e-150 | 429 |
| MS.gene36051.t1 | AT3G18380 | 67.949 | 312 | 82 | 7 | 1 | 294 | 1 | 312 | 6.48e-150 | 427 |
| MS.gene36051.t1 | AT1G15215 | 43.952 | 248 | 133 | 3 | 14 | 259 | 14 | 257 | 2.18e-63 | 203 |
| MS.gene36051.t1 | AT1G15215 | 42.979 | 235 | 128 | 3 | 14 | 246 | 14 | 244 | 1.35e-56 | 186 |
| MS.gene36051.t1 | AT1G15215 | 42.979 | 235 | 128 | 3 | 14 | 246 | 14 | 244 | 1.83e-56 | 185 |
| MS.gene36051.t1 | AT1G15215 | 35.256 | 156 | 92 | 4 | 14 | 167 | 14 | 162 | 9.50e-19 | 83.2 |
Find 89 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTGAGTCATTATCCTTCC+TGG | 0.211207 | 8.1:+52619165 | None:intergenic |
| GAAGGCGGGGCCTCCGTTAC+TGG | 0.217756 | 8.1:+52621440 | None:intergenic |
| TGTAATCTGTAATCAGTTTC+AGG | 0.218700 | 8.1:+52614305 | None:intergenic |
| GAAACATTTGTTTGCAAAAC+AGG | 0.257239 | 8.1:+52614134 | None:intergenic |
| AAAATAAGCGATATGCTATA+AGG | 0.259455 | 8.1:-52619251 | MS.gene36051:CDS |
| GTTCCTGCTCCTTCAGCTTC+AGG | 0.261491 | 8.1:-52619129 | MS.gene36051:intron |
| AAGCACTTGCAGACAAGTTT+AGG | 0.288349 | 8.1:-52619511 | MS.gene36051:intron |
| GGCGTGATATTTAACTTTGC+AGG | 0.330713 | 8.1:+52619210 | None:intergenic |
| CTTTCTATCATACAGACATT+TGG | 0.352765 | 8.1:-52616369 | MS.gene36051:CDS |
| ATGGAATTTGAAGCTAAATC+TGG | 0.358674 | 8.1:-52616514 | MS.gene36051:CDS |
| CTCGCACCATGAACATTTGC+TGG | 0.369463 | 8.1:+52614221 | None:intergenic |
| TTTCATTGCAGGTATGGAAT+TGG | 0.369862 | 8.1:-52619278 | MS.gene36051:intron |
| GTTGAGTCATTATCCTTCCT+GGG | 0.392666 | 8.1:+52619166 | None:intergenic |
| CCAGTGAATGCGGCGGTGCT+TGG | 0.419350 | 8.1:+52614032 | None:intergenic |
| ATCTGTATAGGTAACAGAAA+TGG | 0.427116 | 8.1:-52619585 | MS.gene36051:intron |
| GGATCCACATTCATACTTTG+TGG | 0.428698 | 8.1:+52614104 | None:intergenic |
| AAATAAGCGATATGCTATAA+GGG | 0.442347 | 8.1:-52619250 | MS.gene36051:CDS |
| CCAAGCACCGCCGCATTCAC+TGG | 0.450582 | 8.1:-52614032 | MS.gene36051:CDS |
| GGCTGAGTGAAACGGAAGGC+GGG | 0.454503 | 8.1:+52621426 | None:intergenic |
| GGTTACTAATCCTTCTGAAA+TGG | 0.459416 | 8.1:-52614198 | MS.gene36051:CDS |
| TGTAATCTTGCCTTTACGAT+CGG | 0.459596 | 8.1:+52619379 | None:intergenic |
| CGCAATCTTCTGCTGATCCG+TGG | 0.463686 | 8.1:+52614253 | None:intergenic |
| GAAGCTGAAGGAGCAGGAAC+AGG | 0.470238 | 8.1:+52619132 | None:intergenic |
| GTAATCTTGCCTTTACGATC+GGG | 0.473723 | 8.1:+52619380 | None:intergenic |
| GTCTTTGAGCATCAAGGACA+TGG | 0.475882 | 8.1:+52615557 | None:intergenic |
| ATATTCAAGTTACTTCCCTT+CGG | 0.477909 | 8.1:+52613962 | None:intergenic |
| GACATGGGCATCAAAGTAAA+GGG | 0.481099 | 8.1:+52615573 | None:intergenic |
| CAAATGTCTGTATGATAGAA+AGG | 0.481293 | 8.1:+52616370 | None:intergenic |
| GTCAAATCAGTCCGAGGCAT+GGG | 0.481986 | 8.1:+52619189 | None:intergenic |
| GATCATCCAGCAAATGTTCA+TGG | 0.483799 | 8.1:-52614227 | MS.gene36051:CDS |
| CGGGGCCTCCGTTACTGGGT+GGG | 0.497571 | 8.1:+52621445 | None:intergenic |
| GATTACAGTACAGATGAAAC+AGG | 0.498566 | 8.1:-52619363 | MS.gene36051:intron |
| GGACATGGGCATCAAAGTAA+AGG | 0.499318 | 8.1:+52615572 | None:intergenic |
| GCCAGGAAATCGTGCCGCTT+AGG | 0.503677 | 8.1:-52614343 | MS.gene36051:intron |
| AAGGCGGGGCCTCCGTTACT+GGG | 0.508017 | 8.1:+52621441 | None:intergenic |
| ACAAAAGCTGATGTTCAAGC+TGG | 0.509935 | 8.1:-52614068 | MS.gene36051:CDS |
| CTACCTGAAGCTGAAGGAGC+AGG | 0.516322 | 8.1:+52619126 | None:intergenic |
| GAAGCTAAATCTGGAAGAGA+TGG | 0.517358 | 8.1:-52616505 | MS.gene36051:CDS |
| CCTCGGACTGATTTGACCCC+AGG | 0.520581 | 8.1:-52619183 | MS.gene36051:CDS |
| CAAGTTACTTCCCTTCGGCC+AGG | 0.523928 | 8.1:+52613967 | None:intergenic |
| ATACCTCGGGCTGAGTGAAA+CGG | 0.535015 | 8.1:+52621418 | None:intergenic |
| ACATTTGGAGAGCAGTGATC+CGG | 0.538548 | 8.1:-52616354 | MS.gene36051:intron |
| AGACGGAGGCATGATGTAAG+AGG | 0.539794 | 8.1:-52615538 | MS.gene36051:CDS |
| TAAATATCACGCCCATGCCT+CGG | 0.541650 | 8.1:-52619200 | MS.gene36051:CDS |
| AAACAAAGTATGAGATCACC+AGG | 0.544394 | 8.1:+52615737 | None:intergenic |
| TGCCGCTTAGGAAGATTTGT+CGG | 0.552355 | 8.1:-52614331 | MS.gene36051:CDS |
| GCAATCTTCTGCTGATCCGT+GGG | 0.554224 | 8.1:+52614254 | None:intergenic |
| GCTGAGTGAAACGGAAGGCG+GGG | 0.554948 | 8.1:+52621427 | None:intergenic |
| GCGGGGCCTCCGTTACTGGG+TGG | 0.555777 | 8.1:+52621444 | None:intergenic |
| AGTGAATCACCCGATCGTAA+AGG | 0.556434 | 8.1:-52619389 | MS.gene36051:CDS |
| GGCTCCTGAAAATTCAGTTA+TGG | 0.559539 | 8.1:-52616533 | MS.gene36051:CDS |
| ATGGGTCGCCCACCCAGTAA+CGG | 0.566642 | 8.1:-52621453 | MS.gene36051:CDS |
| GCTTGAAGAACATCTCGTGC+TGG | 0.567998 | 8.1:+52619530 | None:intergenic |
| CAGCAAATGTTCATGGTGCG+AGG | 0.569607 | 8.1:-52614220 | MS.gene36051:CDS |
| GGTCAAATCAGTCCGAGGCA+TGG | 0.569910 | 8.1:+52619188 | None:intergenic |
| GGGCTGAGTGAAACGGAAGG+CGG | 0.572841 | 8.1:+52621425 | None:intergenic |
| GAATGTGTCGCAGTGCTCCC+TGG | 0.583022 | 8.1:-52615755 | MS.gene36051:CDS |
| ATTAATCCACTCATCTTCCT+CGG | 0.585484 | 8.1:+52615823 | None:intergenic |
| CTACTCAAAACCTGGCCGAA+GGG | 0.589785 | 8.1:-52613977 | MS.gene36051:CDS |
| TCTACTCAAAACCTGGCCGA+AGG | 0.590738 | 8.1:-52613978 | MS.gene36051:CDS |
| GCCTCCGTCTTTGAGCATCA+AGG | 0.590882 | 8.1:+52615551 | None:intergenic |
| TTGAGTCATTATCCTTCCTG+GGG | 0.591497 | 8.1:+52619167 | None:intergenic |
| GCAGGAACAGGAGAAGCTGT+TGG | 0.594628 | 8.1:+52619144 | None:intergenic |
| GACTTCAGGAACACTGCTAG+TGG | 0.595794 | 8.1:+52614001 | None:intergenic |
| TGAAGTCTCTACTCAAAACC+TGG | 0.601287 | 8.1:-52613985 | MS.gene36051:CDS |
| GGCAATGATTCCAGTGAATG+CGG | 0.603755 | 8.1:+52614022 | None:intergenic |
| CACAGTTCAAACAACAGCAA+AGG | 0.607569 | 8.1:-52616557 | MS.gene36051:intron |
| ATGTCCTTGATGCTCAAAGA+CGG | 0.608783 | 8.1:-52615555 | MS.gene36051:CDS |
| AGCAAATGTTCATGGTGCGA+GGG | 0.609406 | 8.1:-52614219 | MS.gene36051:CDS |
| GGATCAGCAGAAGATTGCGT+TGG | 0.610789 | 8.1:-52614249 | MS.gene36051:CDS |
| AACATTCTACCTGAAGCTGA+AGG | 0.613833 | 8.1:+52619120 | None:intergenic |
| TCCACATTCATACTTTGTGG+TGG | 0.615610 | 8.1:+52614107 | None:intergenic |
| TTGGATCCGAGGAAGATGAG+TGG | 0.615863 | 8.1:-52615829 | MS.gene36051:CDS |
| ACCACCACAAAGTATGAATG+TGG | 0.618600 | 8.1:-52614108 | MS.gene36051:CDS |
| CTTCCGTTTCACTCAGCCCG+AGG | 0.628138 | 8.1:-52621421 | MS.gene36051:intron |
| AGTTCAAACAACAGCAAAGG+CGG | 0.631634 | 8.1:-52616554 | MS.gene36051:CDS |
| TGTGAATGATGCAGCACCCA+CGG | 0.641428 | 8.1:-52614270 | MS.gene36051:CDS |
| CTATGATCACGATCAATCTG+AGG | 0.641893 | 8.1:-52615491 | MS.gene36051:intron |
| AATCTGGAAGAGATGGAGCA+TGG | 0.658527 | 8.1:-52616498 | MS.gene36051:intron |
| CTCCGACAAATCTTCCTAAG+CGG | 0.665093 | 8.1:+52614329 | None:intergenic |
| CCTGGGGTCAAATCAGTCCG+AGG | 0.675720 | 8.1:+52619183 | None:intergenic |
| TCCTTGATGCTCAAAGACGG+AGG | 0.675814 | 8.1:-52615552 | MS.gene36051:CDS |
| GGACTGATTTGACCCCAGGA+AGG | 0.679224 | 8.1:-52619179 | MS.gene36051:CDS |
| GGTCGCCCACCCAGTAACGG+AGG | 0.681262 | 8.1:-52621450 | MS.gene36051:CDS |
| ACACATTCTGATGATTCACA+TGG | 0.682055 | 8.1:+52615770 | None:intergenic |
| TCTTTGAGCATCAAGGACAT+GGG | 0.687814 | 8.1:+52615558 | None:intergenic |
| CTCGGGCTGAGTGAAACGGA+AGG | 0.689498 | 8.1:+52621422 | None:intergenic |
| AATGATTCCAGTGAATGCGG+CGG | 0.736741 | 8.1:+52614025 | None:intergenic |
| AACAAAGTATGAGATCACCA+GGG | 0.773298 | 8.1:+52615738 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 52613971 | 52621475 | 52613971 | ID=MS.gene36051 |
| chr8.1 | mRNA | 52613971 | 52621475 | 52613971 | ID=MS.gene36051.t1;Parent=MS.gene36051 |
| chr8.1 | exon | 52621422 | 52621475 | 52621422 | ID=MS.gene36051.t1.exon1;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52621422 | 52621475 | 52621422 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52619512 | 52619597 | 52619512 | ID=MS.gene36051.t1.exon2;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52619512 | 52619597 | 52619512 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52619364 | 52619409 | 52619364 | ID=MS.gene36051.t1.exon3;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52619364 | 52619409 | 52619364 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52619130 | 52619289 | 52619130 | ID=MS.gene36051.t1.exon4;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52619130 | 52619289 | 52619130 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52616499 | 52616574 | 52616499 | ID=MS.gene36051.t1.exon5;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52616499 | 52616574 | 52616499 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52616355 | 52616406 | 52616355 | ID=MS.gene36051.t1.exon6;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52616355 | 52616406 | 52616355 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52615733 | 52615876 | 52615733 | ID=MS.gene36051.t1.exon7;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52615733 | 52615876 | 52615733 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52615492 | 52615611 | 52615492 | ID=MS.gene36051.t1.exon8;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52615492 | 52615611 | 52615492 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| chr8.1 | exon | 52613971 | 52614360 | 52613971 | ID=MS.gene36051.t1.exon9;Parent=MS.gene36051.t1 |
| chr8.1 | CDS | 52613971 | 52614360 | 52613971 | ID=cds.MS.gene36051.t1;Parent=MS.gene36051.t1 |
| Gene Sequence |
| Protein sequence |
