AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36460.t1 MTR_4g094572 98.408 377 6 0 1 377 1 377 0.0 765
MS.gene36460.t1 MTR_5g077710 43.144 299 148 7 91 375 94 384 1.07e-78 247
MS.gene36460.t1 MTR_2g028590 40.604 298 156 7 91 375 174 463 3.29e-76 243
MS.gene36460.t1 MTR_2g028610 42.088 297 151 8 92 375 170 458 7.40e-76 242
MS.gene36460.t1 MTR_1g024005 32.692 416 226 8 4 375 19 424 1.03e-73 235
MS.gene36460.t1 MTR_3g113270 32.117 411 230 10 4 375 19 419 1.31e-70 227
MS.gene36460.t1 MTR_0010s0400 41.096 292 151 7 92 374 177 456 4.65e-62 206
MS.gene36460.t1 MTR_8g028460 38.158 304 161 8 89 374 191 485 5.55e-59 199
MS.gene36460.t1 MTR_3g071050 37.647 170 89 5 89 248 103 265 3.24e-29 114
MS.gene36460.t1 MTR_3g070620 39.130 138 72 4 178 313 62 189 9.13e-24 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36460.t1 AT1G29790 67.008 391 92 8 7 377 5 378 0.0 516
MS.gene36460.t1 AT1G29790 67.008 391 92 8 7 377 5 378 0.0 516
MS.gene36460.t1 AT5G40830 34.230 409 215 11 4 374 19 411 3.78e-73 234
MS.gene36460.t1 AT5G40830 34.230 409 215 11 4 374 19 411 3.78e-73 234
MS.gene36460.t1 AT3G27230 33.580 405 221 9 4 375 19 408 3.51e-70 226
MS.gene36460.t1 AT5G40830 33.251 403 187 10 4 374 19 371 1.53e-67 218
MS.gene36460.t1 AT3G05390 40.753 292 152 7 92 374 180 459 7.27e-66 216
MS.gene36460.t1 AT4G01240 36.964 303 165 7 89 374 361 654 2.09e-57 198

Find 103 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTGATTCCTCTGTTATC+TGG 0.165102 4.1:+18659562 MS.gene36460:CDS
TAAGCCTTTGATTGCGAAAA+TGG 0.167124 4.1:+18660085 MS.gene36460:CDS
GGGATTGGTGGAAGTGGTTT+TGG 0.169419 4.1:-18659195 None:intergenic
TGGTGTTAGGGTTGGTGTTT+TGG 0.217335 4.1:-18659278 None:intergenic
CTTGATGTTGGTGGTGGTAC+TGG 0.282533 4.1:+18659756 MS.gene36460:CDS
GAGGCGCGTGAGGTGGTTAA+TGG 0.310317 4.1:-18659256 None:intergenic
CTCGATTTGTCCTTCTGATT+CGG 0.339448 4.1:+18659416 MS.gene36460:CDS
TGGTTGCTACCATTGCTATT+AGG 0.349162 4.1:-18659122 None:intergenic
CAGTGAGGCGGTTGCGTTAA+GGG 0.357505 4.1:+18659851 MS.gene36460:CDS
GATCGCGAAATCAGCGAAAT+CGG 0.372554 4.1:+18659719 MS.gene36460:CDS
ATCTGAAGAAGCTGTTGAAC+TGG 0.377068 4.1:-18659699 None:intergenic
ACCTGATTCCTCTGTTATCT+GGG 0.386515 4.1:+18659563 MS.gene36460:CDS
GGCGGTGAATCGATGGATTC+CGG 0.389269 4.1:+18659953 MS.gene36460:CDS
TTGGACGGAGTTTGTTTCTT+CGG 0.393300 4.1:-18659507 None:intergenic
AAGAAAGTGAAGTGGGCAAC+GGG 0.410418 4.1:+18660113 MS.gene36460:CDS
TGAGAGGTGGAGGGTATTTG+TGG 0.411452 4.1:+18660018 MS.gene36460:CDS
GAGTAGATCTGAAGGGATTG+AGG 0.412355 4.1:-18659310 None:intergenic
GAAGGTTCAAATCCGAGATC+AGG 0.415306 4.1:-18659630 None:intergenic
ACAGTGAGGCGGTTGCGTTA+AGG 0.420442 4.1:+18659850 MS.gene36460:CDS
ATGTACTTGTGAAGAAGATC+AGG 0.423383 4.1:-18659378 None:intergenic
CAGCGGTTACCGATGTTTGA+TGG 0.424081 4.1:+18659903 MS.gene36460:CDS
CGGAGGAGTTCGTCGGGGAT+TGG 0.428015 4.1:-18659210 None:intergenic
TTACGGCTGGGGCTTGATGT+TGG 0.437399 4.1:+18659744 MS.gene36460:CDS
AAACAACGCCGGCGAGGGAG+AGG 0.443660 4.1:-18659474 None:intergenic
GAGTAGAGGAGTAGATCTGA+AGG 0.444596 4.1:-18659318 None:intergenic
CCATTGCTATTAGGAGAAGA+AGG 0.457866 4.1:-18659113 None:intergenic
CGGCTGGGGCTTGATGTTGG+TGG 0.458823 4.1:+18659747 MS.gene36460:CDS
TCAGGTAAGGAAGAAGGAAA+AGG 0.465159 4.1:-18659546 None:intergenic
AAGCCTTTGATTGCGAAAAT+GGG 0.467826 4.1:+18660086 MS.gene36460:CDS
AACGCAACCGCCTCACTGTA+AGG 0.473749 4.1:-18659846 None:intergenic
ATCGGTAACCGCTGCTGCAA+CGG 0.476365 4.1:-18659894 None:intergenic
TGGTGGTACTGGCTCATTTG+CGG 0.479932 4.1:+18659767 MS.gene36460:CDS
CAGGTAAGGAAGAAGGAAAA+GGG 0.483236 4.1:-18659545 None:intergenic
GTGGGAGGGCGGTGAATCGA+TGG 0.484047 4.1:+18659946 MS.gene36460:CDS
AACCGTCAAAACCCTGATCT+CGG 0.488287 4.1:+18659618 MS.gene36460:CDS
AGTTGACGGAGGAGTTCGTC+GGG 0.488936 4.1:-18659216 None:intergenic
GCTGAAAGCTTAATTCTTCG+CGG 0.489356 4.1:+18659444 MS.gene36460:CDS
TCCCAGATAACAGAGGAATC+AGG 0.491176 4.1:-18659564 None:intergenic
TTTAGGGGATTGGAGAGTTG+TGG 0.496838 4.1:-18659169 None:intergenic
ATTGGAGAGTTGTGGAGAGG+TGG 0.498315 4.1:-18659161 None:intergenic
GTGGTAGAGAGAGAGAATGT+TGG 0.507068 4.1:-18659142 None:intergenic
TCGTCGGGGATTGGTGGAAG+TGG 0.507708 4.1:-18659201 None:intergenic
GAGGAATCAGGTAAGGAAGA+AGG 0.511266 4.1:-18659552 None:intergenic
AGGAGTTCGTCGGGGATTGG+TGG 0.516755 4.1:-18659207 None:intergenic
GATAGAGTGTTGAGAGGTGG+AGG 0.517077 4.1:+18660008 MS.gene36460:CDS
CTGGGGCTTGATGTTGGTGG+TGG 0.522249 4.1:+18659750 MS.gene36460:CDS
ATGGTGTCGTGGATATTGTT+CGG 0.522500 4.1:+18659922 MS.gene36460:CDS
ATGGATTCCGGTGACGATGT+TGG 0.523220 4.1:+18659965 MS.gene36460:CDS
TTGGTGAGAGGTGTGAGTAG+AGG 0.528167 4.1:-18659332 None:intergenic
ACGGGGAATAAGACTGATGC+TGG 0.528181 4.1:+18660131 MS.gene36460:CDS
TCGTGGATATTGTTCGGTGT+GGG 0.528274 4.1:+18659928 MS.gene36460:CDS
AGGTGGAGGGTATTTGTGGT+TGG 0.528302 4.1:+18660022 MS.gene36460:CDS
CGCGTATAGTGTGAAGTTGA+CGG 0.530863 4.1:-18659230 None:intergenic
AGTAGAGGAGTAGATCTGAA+GGG 0.535223 4.1:-18659317 None:intergenic
TGCTGCAACGGAACATGAAG+CGG 0.536548 4.1:-18659882 None:intergenic
GGATATTGTTCGGTGTGGGA+GGG 0.536790 4.1:+18659932 MS.gene36460:CDS
TTATGACAAGAAGAAGCAAT+TGG 0.537441 4.1:-18659351 None:intergenic
TTGCGGCGGCGATGAAAGTA+CGG 0.539661 4.1:+18659784 MS.gene36460:CDS
GATAACAGAGGAATCAGGTA+AGG 0.541159 4.1:-18659559 None:intergenic
TGGATATTGTTCGGTGTGGG+AGG 0.543086 4.1:+18659931 MS.gene36460:CDS
GAGAAGAAGGTTTAGTGTCA+TGG 0.552063 4.1:-18659100 None:intergenic
GAGAGAAACAACGCCGGCGA+GGG 0.553700 4.1:-18659479 None:intergenic
AAGGTTCAAATCCGAGATCA+GGG 0.554632 4.1:-18659629 None:intergenic
TATTGTTCGGTGTGGGAGGG+CGG 0.561682 4.1:+18659935 MS.gene36460:CDS
AGTGAGGCGGTTGCGTTAAG+GGG 0.563157 4.1:+18659852 MS.gene36460:CDS
TCTGAAGAAGCTGTTGAACT+GGG 0.564387 4.1:-18659698 None:intergenic
TTCATGTTCCGTTGCAGCAG+CGG 0.564533 4.1:+18659886 MS.gene36460:CDS
GGGTTACAAGAAAGTGAAGT+GGG 0.565136 4.1:+18660106 MS.gene36460:CDS
GGAGTTTGTTTCTTCGGCGT+CGG 0.566366 4.1:-18659501 None:intergenic
CAAGAAAGTGAAGTGGGCAA+CGG 0.572632 4.1:+18660112 MS.gene36460:CDS
GTTGTCATCCTCTCCCTCGC+CGG 0.574392 4.1:+18659466 MS.gene36460:CDS
AACTTAGAAACTTCACGAGA+AGG 0.584495 4.1:-18659648 None:intergenic
GATGTTGATAGAGTGTTGAG+AGG 0.584870 4.1:+18660002 MS.gene36460:CDS
CCTTCTTCTCCTAATAGCAA+TGG 0.585257 4.1:+18659113 MS.gene36460:CDS
AAGTTGACGGAGGAGTTCGT+CGG 0.588114 4.1:-18659217 None:intergenic
GTCGTGGATATTGTTCGGTG+TGG 0.593818 4.1:+18659927 MS.gene36460:CDS
GGGATTGGAGAGTTGTGGAG+AGG 0.598949 4.1:-18659164 None:intergenic
GATGAAAGTACGGAATGTTA+CGG 0.601069 4.1:+18659794 MS.gene36460:CDS
GCGCGTGAGGTGGTTAATGG+TGG 0.606953 4.1:-18659253 None:intergenic
TCAGCGAGCTCCGAATCAGA+AGG 0.610853 4.1:-18659426 None:intergenic
ACCGATGTTTGATGGTGTCG+TGG 0.615004 4.1:+18659911 MS.gene36460:CDS
GATGCTGGTGGTGTTAAGCA+TGG 0.624372 4.1:+18660146 MS.gene36460:CDS
GGAGAGAAACAACGCCGGCG+AGG 0.631888 4.1:-18659480 None:intergenic
GTTGATAGAGTGTTGAGAGG+TGG 0.635445 4.1:+18660005 MS.gene36460:CDS
AAGAAGAAGCAATTGGTGAG+AGG 0.636322 4.1:-18659344 None:intergenic
TGTGACTCTCATCTTGAGAG+AGG 0.637895 4.1:-18660200 None:intergenic
ATAGAGTGTTGAGAGGTGGA+GGG 0.639182 4.1:+18660009 MS.gene36460:CDS
ACAAATCGAGAACGGTGTGT+AGG 0.640075 4.1:-18659403 None:intergenic
TGGGTTACAAGAAAGTGAAG+TGG 0.640685 4.1:+18660105 MS.gene36460:CDS
TCAGAAGGACAAATCGAGAA+CGG 0.666336 4.1:-18659411 None:intergenic
TCCACGACACCATCAAACAT+CGG 0.670096 4.1:-18659912 None:intergenic
TGGTACTGGCTCATTTGCGG+CGG 0.680808 4.1:+18659770 MS.gene36460:CDS
TGTACTTGTGAAGAAGATCA+GGG 0.686850 4.1:-18659377 None:intergenic
TGTAGCGCCTTACAGTGAGG+CGG 0.694193 4.1:+18659839 MS.gene36460:CDS
TGTTGTAGCGCCTTACAGTG+AGG 0.710000 4.1:+18659836 MS.gene36460:CDS
GAAAGTACGGAATGTTACGG+TGG 0.724848 4.1:+18659797 MS.gene36460:CDS
GCGTCGGAGAGAAACAACGC+CGG 0.732120 4.1:-18659485 None:intergenic
ATAACGATCCCAGATAACAG+AGG 0.735131 4.1:-18659571 None:intergenic
GTTGACGGAGGAGTTCGTCG+GGG 0.738259 4.1:-18659215 None:intergenic
AGAAAGTGAAGTGGGCAACG+GGG 0.746580 4.1:+18660114 MS.gene36460:CDS
GTATAGTGTGAAGTTGACGG+AGG 0.747585 4.1:-18659227 None:intergenic
GGGAATAAGACTGATGCTGG+TGG 0.762122 4.1:+18660134 MS.gene36460:CDS
ACTTGTGAAGAAGATCAGGG+TGG 0.798354 4.1:-18659374 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGGACCATTTTTTTAGTAA+GGG + chr4.1:18660041-18660060 MS.gene36460:CDS 25.0%
TTATGACAAGAAGAAGCAAT+TGG - chr4.1:18659354-18659373 None:intergenic 30.0%
!!! GTTGGACCATTTTTTTAGTA+AGG + chr4.1:18660040-18660059 MS.gene36460:CDS 30.0%
AACTTAGAAACTTCACGAGA+AGG - chr4.1:18659651-18659670 None:intergenic 35.0%
AAGCCTTTGATTGCGAAAAT+GGG + chr4.1:18660086-18660105 MS.gene36460:CDS 35.0%
ATGTACTTGTGAAGAAGATC+AGG - chr4.1:18659381-18659400 None:intergenic 35.0%
CCACACCCTTACTAAAAAAA+TGG - chr4.1:18660049-18660068 None:intergenic 35.0%
TAAGCCTTTGATTGCGAAAA+TGG + chr4.1:18660085-18660104 MS.gene36460:CDS 35.0%
TGTACTTGTGAAGAAGATCA+GGG - chr4.1:18659380-18659399 None:intergenic 35.0%
! GAAGAAGGAAAAGGGTTTTT+CGG - chr4.1:18659540-18659559 None:intergenic 35.0%
! GATGAAAGTACGGAATGTTA+CGG + chr4.1:18659794-18659813 MS.gene36460:CDS 35.0%
! TAACCCATTTTCGCAATCAA+AGG - chr4.1:18660092-18660111 None:intergenic 35.0%
!! AATCAGCGAAATCGGTTTTA+CGG + chr4.1:18659727-18659746 MS.gene36460:CDS 35.0%
!!! AGGATTTTGTGTTGGTGTTA+GGG - chr4.1:18659293-18659312 None:intergenic 35.0%
!!! CCATTTTTTTAGTAAGGGTG+TGG + chr4.1:18660046-18660065 MS.gene36460:CDS 35.0%
AAAAACTCCAACATCGTCAC+CGG - chr4.1:18659975-18659994 None:intergenic 40.0%
AAGAAGAAGCAATTGGTGAG+AGG - chr4.1:18659347-18659366 None:intergenic 40.0%
AAGGTTCAAATCCGAGATCA+GGG - chr4.1:18659632-18659651 None:intergenic 40.0%
ACCTGATTCCTCTGTTATCT+GGG + chr4.1:18659563-18659582 MS.gene36460:CDS 40.0%
AGTAGAGGAGTAGATCTGAA+GGG - chr4.1:18659320-18659339 None:intergenic 40.0%
ATAACGATCCCAGATAACAG+AGG - chr4.1:18659574-18659593 None:intergenic 40.0%
ATCTGAAGAAGCTGTTGAAC+TGG - chr4.1:18659702-18659721 None:intergenic 40.0%
CAGGTAAGGAAGAAGGAAAA+GGG - chr4.1:18659548-18659567 None:intergenic 40.0%
CCTTCTTCTCCTAATAGCAA+TGG + chr4.1:18659113-18659132 MS.gene36460:CDS 40.0%
GAGAAGAAGGTTTAGTGTCA+TGG - chr4.1:18659103-18659122 None:intergenic 40.0%
GATAACAGAGGAATCAGGTA+AGG - chr4.1:18659562-18659581 None:intergenic 40.0%
GCTGAAAGCTTAATTCTTCG+CGG + chr4.1:18659444-18659463 MS.gene36460:CDS 40.0%
GGGTTACAAGAAAGTGAAGT+GGG + chr4.1:18660106-18660125 MS.gene36460:CDS 40.0%
TACCTGATTCCTCTGTTATC+TGG + chr4.1:18659562-18659581 MS.gene36460:CDS 40.0%
TCAGAAGGACAAATCGAGAA+CGG - chr4.1:18659414-18659433 None:intergenic 40.0%
TCAGGTAAGGAAGAAGGAAA+AGG - chr4.1:18659549-18659568 None:intergenic 40.0%
TCTGAAGAAGCTGTTGAACT+GGG - chr4.1:18659701-18659720 None:intergenic 40.0%
TGGGTTACAAGAAAGTGAAG+TGG + chr4.1:18660105-18660124 MS.gene36460:CDS 40.0%
! AGGGATTGAGGATTTTGTGT+TGG - chr4.1:18659301-18659320 None:intergenic 40.0%
! CCATTGCTATTAGGAGAAGA+AGG - chr4.1:18659116-18659135 None:intergenic 40.0%
! CTCGATTTGTCCTTCTGATT+CGG + chr4.1:18659416-18659435 MS.gene36460:CDS 40.0%
! TGGTTGCTACCATTGCTATT+AGG - chr4.1:18659125-18659144 None:intergenic 40.0%
! TTGGACGGAGTTTGTTTCTT+CGG - chr4.1:18659510-18659529 None:intergenic 40.0%
!! ATGGTGTCGTGGATATTGTT+CGG + chr4.1:18659922-18659941 MS.gene36460:CDS 40.0%
!! GAGGATTTTGTGTTGGTGTT+AGG - chr4.1:18659294-18659313 None:intergenic 40.0%
!! GATGTTGATAGAGTGTTGAG+AGG + chr4.1:18660002-18660021 MS.gene36460:CDS 40.0%
!! TTTTTCGGAAGCGAATTGGA+CGG - chr4.1:18659525-18659544 None:intergenic 40.0%
!!! AAGTGGTTTTGGGGATTTAG+GGG - chr4.1:18659187-18659206 None:intergenic 40.0%
!!! GAAGTGGTTTTGGGGATTTA+GGG - chr4.1:18659188-18659207 None:intergenic 40.0%
!!! TTTTGTGTTGGTGTTAGGGT+TGG - chr4.1:18659289-18659308 None:intergenic 40.0%
AACCGTCAAAACCCTGATCT+CGG + chr4.1:18659618-18659637 MS.gene36460:CDS 45.0%
AAGAAAGTGAAGTGGGCAAC+GGG + chr4.1:18660113-18660132 MS.gene36460:CDS 45.0%
ACAAATCGAGAACGGTGTGT+AGG - chr4.1:18659406-18659425 None:intergenic 45.0%
ACTTGTGAAGAAGATCAGGG+TGG - chr4.1:18659377-18659396 None:intergenic 45.0%
CAAGAAAGTGAAGTGGGCAA+CGG + chr4.1:18660112-18660131 MS.gene36460:CDS 45.0%
CGCGTATAGTGTGAAGTTGA+CGG - chr4.1:18659233-18659252 None:intergenic 45.0%
GAAGGTTCAAATCCGAGATC+AGG - chr4.1:18659633-18659652 None:intergenic 45.0%
GAGGAATCAGGTAAGGAAGA+AGG - chr4.1:18659555-18659574 None:intergenic 45.0%
GAGTAGAGGAGTAGATCTGA+AGG - chr4.1:18659321-18659340 None:intergenic 45.0%
GAGTAGATCTGAAGGGATTG+AGG - chr4.1:18659313-18659332 None:intergenic 45.0%
GATCGCGAAATCAGCGAAAT+CGG + chr4.1:18659719-18659738 MS.gene36460:CDS 45.0%
GTATAGTGTGAAGTTGACGG+AGG - chr4.1:18659230-18659249 None:intergenic 45.0%
GTGGTAGAGAGAGAGAATGT+TGG - chr4.1:18659145-18659164 None:intergenic 45.0%
TCCACGACACCATCAAACAT+CGG - chr4.1:18659915-18659934 None:intergenic 45.0%
TCCCAGATAACAGAGGAATC+AGG - chr4.1:18659567-18659586 None:intergenic 45.0%
TTTAGGGGATTGGAGAGTTG+TGG - chr4.1:18659172-18659191 None:intergenic 45.0%
! GAAAGTACGGAATGTTACGG+TGG + chr4.1:18659797-18659816 MS.gene36460:CDS 45.0%
!! AGGGTTTTTCGGAAGCGAAT+TGG - chr4.1:18659529-18659548 None:intergenic 45.0%
!! ATAGAGTGTTGAGAGGTGGA+GGG + chr4.1:18660009-18660028 MS.gene36460:CDS 45.0%
!! ATCCGAGATCAGGGTTTTGA+CGG - chr4.1:18659623-18659642 None:intergenic 45.0%
!! GATTGGTGGAAGTGGTTTTG+GGG - chr4.1:18659196-18659215 None:intergenic 45.0%
!! GGATTGGTGGAAGTGGTTTT+GGG - chr4.1:18659197-18659216 None:intergenic 45.0%
!! GTTGATAGAGTGTTGAGAGG+TGG + chr4.1:18660005-18660024 MS.gene36460:CDS 45.0%
!! TCGTGGATATTGTTCGGTGT+GGG + chr4.1:18659928-18659947 MS.gene36460:CDS 45.0%
!!! GGAAGTGGTTTTGGGGATTT+AGG - chr4.1:18659189-18659208 None:intergenic 45.0%
!!! GTTTTGGGGATTTAGGGGAT+TGG - chr4.1:18659182-18659201 None:intergenic 45.0%
!!! TGGTGTTAGGGTTGGTGTTT+TGG - chr4.1:18659281-18659300 None:intergenic 45.0%
!!! TGTTAGGGTTGGTGTTTTGG+AGG - chr4.1:18659278-18659297 None:intergenic 45.0%
AAGTTGACGGAGGAGTTCGT+CGG - chr4.1:18659220-18659239 None:intergenic 50.0%
ACGGGGAATAAGACTGATGC+TGG + chr4.1:18660131-18660150 MS.gene36460:CDS 50.0%
AGAAAGTGAAGTGGGCAACG+GGG + chr4.1:18660114-18660133 MS.gene36460:CDS 50.0%
ATGGATTCCGGTGACGATGT+TGG + chr4.1:18659965-18659984 MS.gene36460:CDS 50.0%
ATTGGAGAGTTGTGGAGAGG+TGG - chr4.1:18659164-18659183 None:intergenic 50.0%
CAGCGGTTACCGATGTTTGA+TGG + chr4.1:18659903-18659922 MS.gene36460:CDS 50.0%
GGGAATAAGACTGATGCTGG+TGG + chr4.1:18660134-18660153 MS.gene36460:CDS 50.0%
TGCTGCAACGGAACATGAAG+CGG - chr4.1:18659885-18659904 None:intergenic 50.0%
TGTTGTAGCGCCTTACAGTG+AGG + chr4.1:18659836-18659855 MS.gene36460:CDS 50.0%
TTCATGTTCCGTTGCAGCAG+CGG + chr4.1:18659886-18659905 MS.gene36460:CDS 50.0%
! GATGCTGGTGGTGTTAAGCA+TGG + chr4.1:18660146-18660165 MS.gene36460:CDS 50.0%
! GGAGTTTGTTTCTTCGGCGT+CGG - chr4.1:18659504-18659523 None:intergenic 50.0%
! GTCGTGGATATTGTTCGGTG+TGG + chr4.1:18659927-18659946 MS.gene36460:CDS 50.0%
! TTGGTGAGAGGTGTGAGTAG+AGG - chr4.1:18659335-18659354 None:intergenic 50.0%
!! ACCGATGTTTGATGGTGTCG+TGG + chr4.1:18659911-18659930 MS.gene36460:CDS 50.0%
!! AGCGAAATCGGTTTTACGGC+TGG + chr4.1:18659731-18659750 MS.gene36460:CDS 50.0%
!! AGGTGGAGGGTATTTGTGGT+TGG + chr4.1:18660022-18660041 MS.gene36460:CDS 50.0%
!! CGAAATCGGTTTTACGGCTG+GGG + chr4.1:18659733-18659752 MS.gene36460:CDS 50.0%
!! CTTGATGTTGGTGGTGGTAC+TGG + chr4.1:18659756-18659775 MS.gene36460:CDS 50.0%
!! GATAGAGTGTTGAGAGGTGG+AGG + chr4.1:18660008-18660027 MS.gene36460:CDS 50.0%
!! GCGAAATCGGTTTTACGGCT+GGG + chr4.1:18659732-18659751 MS.gene36460:CDS 50.0%
!! GGATATTGTTCGGTGTGGGA+GGG + chr4.1:18659932-18659951 MS.gene36460:CDS 50.0%
!! GGGATTGGTGGAAGTGGTTT+TGG - chr4.1:18659198-18659217 None:intergenic 50.0%
!! TGAGAGGTGGAGGGTATTTG+TGG + chr4.1:18660018-18660037 MS.gene36460:CDS 50.0%
!! TGGATATTGTTCGGTGTGGG+AGG + chr4.1:18659931-18659950 MS.gene36460:CDS 50.0%
!! TGGTGGTACTGGCTCATTTG+CGG + chr4.1:18659767-18659786 MS.gene36460:CDS 50.0%
AACGCAACCGCCTCACTGTA+AGG - chr4.1:18659849-18659868 None:intergenic 55.0%
ACAGTGAGGCGGTTGCGTTA+AGG + chr4.1:18659850-18659869 MS.gene36460:CDS 55.0%
AGTGAGGCGGTTGCGTTAAG+GGG + chr4.1:18659852-18659871 MS.gene36460:CDS 55.0%
AGTTGACGGAGGAGTTCGTC+GGG - chr4.1:18659219-18659238 None:intergenic 55.0%
ATCGGTAACCGCTGCTGCAA+CGG - chr4.1:18659897-18659916 None:intergenic 55.0%
CAGTGAGGCGGTTGCGTTAA+GGG + chr4.1:18659851-18659870 MS.gene36460:CDS 55.0%
GGGATTGGAGAGTTGTGGAG+AGG - chr4.1:18659167-18659186 None:intergenic 55.0%
TCAGCGAGCTCCGAATCAGA+AGG - chr4.1:18659429-18659448 None:intergenic 55.0%
TGTAGCGCCTTACAGTGAGG+CGG + chr4.1:18659839-18659858 MS.gene36460:CDS 55.0%
TTACGGCTGGGGCTTGATGT+TGG + chr4.1:18659744-18659763 MS.gene36460:CDS 55.0%
TTGCGGCGGCGATGAAAGTA+CGG + chr4.1:18659784-18659803 MS.gene36460:CDS 55.0%
! GGCGGTGAATCGATGGATTC+CGG + chr4.1:18659953-18659972 MS.gene36460:CDS 55.0%
!! TATTGTTCGGTGTGGGAGGG+CGG + chr4.1:18659935-18659954 MS.gene36460:CDS 55.0%
!! TGGTACTGGCTCATTTGCGG+CGG + chr4.1:18659770-18659789 MS.gene36460:CDS 55.0%
AGGAGTTCGTCGGGGATTGG+TGG - chr4.1:18659210-18659229 None:intergenic 60.0%
GAGAGAAACAACGCCGGCGA+GGG - chr4.1:18659482-18659501 None:intergenic 60.0%
GAGGCGCGTGAGGTGGTTAA+TGG - chr4.1:18659259-18659278 None:intergenic 60.0%
GCGCGTGAGGTGGTTAATGG+TGG - chr4.1:18659256-18659275 None:intergenic 60.0%
GCGTCGGAGAGAAACAACGC+CGG - chr4.1:18659488-18659507 None:intergenic 60.0%
GTTGACGGAGGAGTTCGTCG+GGG - chr4.1:18659218-18659237 None:intergenic 60.0%
GTTGTCATCCTCTCCCTCGC+CGG + chr4.1:18659466-18659485 MS.gene36460:CDS 60.0%
! CTGGGGCTTGATGTTGGTGG+TGG + chr4.1:18659750-18659769 MS.gene36460:CDS 60.0%
! TCGTCGGGGATTGGTGGAAG+TGG - chr4.1:18659204-18659223 None:intergenic 60.0%
!!! TGGTGTTTTGGAGGCGCGTG+AGG - chr4.1:18659269-18659288 None:intergenic 60.0%
!!! TGTTTTGGAGGCGCGTGAGG+TGG - chr4.1:18659266-18659285 None:intergenic 60.0%
AAACAACGCCGGCGAGGGAG+AGG - chr4.1:18659477-18659496 None:intergenic 65.0%
CGGAGGAGTTCGTCGGGGAT+TGG - chr4.1:18659213-18659232 None:intergenic 65.0%
CGGCTGGGGCTTGATGTTGG+TGG + chr4.1:18659747-18659766 MS.gene36460:CDS 65.0%
GGAGAGAAACAACGCCGGCG+AGG - chr4.1:18659483-18659502 None:intergenic 65.0%
GTGGGAGGGCGGTGAATCGA+TGG + chr4.1:18659946-18659965 MS.gene36460:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 18659081 18660214 18659081 ID=MS.gene36460
chr4.1 mRNA 18659081 18660214 18659081 ID=MS.gene36460.t1;Parent=MS.gene36460
chr4.1 exon 18659081 18660214 18659081 ID=MS.gene36460.t1.exon1;Parent=MS.gene36460.t1
chr4.1 CDS 18659081 18660214 18659081 ID=cds.MS.gene36460.t1;Parent=MS.gene36460.t1
Gene Sequence

>MS.gene36460

ATGGGTTTCACATTCAGCACCATGACACTAAACCTTCTTCTCCTAATAGCAATGGTAGCAACCAACATTCTCTCTCTCTACCACCTCTCCACAACTCTCCAATCCCCTAAATCCCCAAAACCACTTCCACCAATCCCCGACGAACTCCTCCGTCAACTTCACACTATACGCGCCACCATTAACCACCTCACGCGCCTCCAAAACACCAACCCTAACACCAACACAAAATCCTCAATCCCTTCAGATCTACTCCTCTACTCACACCTCTCACCAATTGCTTCTTCTTGTCATAACCACCCTGATCTTCTTCACAAGTACATGACCTACACACCGTTCTCGATTTGTCCTTCTGATTCGGAGCTCGCTGAAAGCTTAATTCTTCGCGGTTGTCATCCTCTCCCTCGCCGGCGTTGTTTCTCTCCGACGCCGAAGAAACAAACTCCGTCCAATTCGCTTCCGAAAAACCCTTTTCCTTCTTCCTTACCTGATTCCTCTGTTATCTGGGATCGTTATTCATGTAAATCATTCGATTGTTTTAACCGTCAAAACCCTGATCTCGGATTTGAACCTTCTCGTGAAGTTTCTAAGTTTACTAGCTACAATACTGACTTAGATCTCCCAGTTCAACAGCTTCTTCAGATCGCGAAATCAGCGAAATCGGTTTTACGGCTGGGGCTTGATGTTGGTGGTGGTACTGGCTCATTTGCGGCGGCGATGAAAGTACGGAATGTTACGGTGGTTACTACGACGATGAATGTTGTAGCGCCTTACAGTGAGGCGGTTGCGTTAAGGGGACTTGTGCCGCTTCATGTTCCGTTGCAGCAGCGGTTACCGATGTTTGATGGTGTCGTGGATATTGTTCGGTGTGGGAGGGCGGTGAATCGATGGATTCCGGTGACGATGTTGGAGTTTTTGTTGTTTGATGTTGATAGAGTGTTGAGAGGTGGAGGGTATTTGTGGTTGGACCATTTTTTTAGTAAGGGTGTGGATCTTGAGAAAGTGTATAAGCCTTTGATTGCGAAAATGGGTTACAAGAAAGTGAAGTGGGCAACGGGGAATAAGACTGATGCTGGTGGTGTTAAGCATGGAGAGCTTTACTTGACTGCATTGCTTCAAAAGCCTCTCTCAAGATGA
Protein sequence

>MS.gene36460.t1

MGFTFSTMTLNLLLLIAMVATNILSLYHLSTTLQSPKSPKPLPPIPDELLRQLHTIRATINHLTRLQNTNPNTNTKSSIPSDLLLYSHLSPIASSCHNHPDLLHKYMTYTPFSICPSDSELAESLILRGCHPLPRRRCFSPTPKKQTPSNSLPKNPFPSSLPDSSVIWDRYSCKSFDCFNRQNPDLGFEPSREVSKFTSYNTDLDLPVQQLLQIAKSAKSVLRLGLDVGGGTGSFAAAMKVRNVTVVTTTMNVVAPYSEAVALRGLVPLHVPLQQRLPMFDGVVDIVRCGRAVNRWIPVTMLEFLLFDVDRVLRGGGYLWLDHFFSKGVDLEKVYKPLIAKMGYKKVKWATGNKTDAGGVKHGELYLTALLQKPLSR