AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene014442


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014442.t1 MTR_3g113270 97.387 421 11 0 1 421 1 421 0.0 848
MS.gene014442.t1 MTR_1g024005 76.457 429 88 5 1 420 1 425 0.0 656
MS.gene014442.t1 MTR_3g071050 97.358 265 7 0 29 293 1 265 0.0 540
MS.gene014442.t1 MTR_3g070620 97.037 135 4 0 225 359 54 188 2.49e-91 274
MS.gene014442.t1 MTR_2g028590 40.053 377 208 8 56 419 92 463 5.20e-89 278
MS.gene014442.t1 MTR_2g028610 37.123 431 224 11 30 419 34 458 9.37e-89 277
MS.gene014442.t1 MTR_5g077710 42.722 316 169 8 113 420 74 385 1.64e-86 269
MS.gene014442.t1 MTR_8g028460 40.785 331 175 7 104 420 164 487 4.02e-82 261
MS.gene014442.t1 MTR_0010s0400 41.667 324 172 9 104 421 147 459 6.35e-82 259
MS.gene014442.t1 MTR_4g094572 32.847 411 227 9 19 419 4 375 1.78e-62 206
MS.gene014442.t1 MTR_3g071100 100.000 41 0 0 204 244 23 63 1.49e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene014442.t1 AT3G27230 70.071 421 115 6 1 421 1 410 0.0 599
MS.gene014442.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene014442.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene014442.t1 AT5G40830 62.796 422 108 5 1 421 1 374 0.0 530
MS.gene014442.t1 AT3G05390 40.741 324 175 8 104 421 150 462 7.27e-84 264
MS.gene014442.t1 AT4G01240 41.667 348 173 10 89 420 323 656 9.22e-80 259
MS.gene014442.t1 AT1G29790 33.835 399 232 7 24 419 7 376 2.61e-71 229
MS.gene014442.t1 AT1G29790 33.835 399 232 7 24 419 7 376 2.61e-71 229

Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGTTTCCAAAGTGAATT+TGG 0.174013 3.1:-89982540 None:intergenic
CATTTCAGTGATTCCAGTTT+TGG 0.201099 3.1:-89982322 None:intergenic
CTGTTCTTCAGAAGCCTGTT+AGG 0.201673 3.1:+89983192 MS.gene014442:CDS
TTAGCAAAACACCTTCTTCT+AGG 0.243776 3.1:-89982480 None:intergenic
TGCTAAATCTGTTTCTTCTA+AGG 0.249692 3.1:+89982497 MS.gene014442:CDS
GGTTCTGTTTCTTTGAAAAT+TGG 0.270644 3.1:+89981958 MS.gene014442:CDS
TTTGATATTGGAATTGGTTC+TGG 0.272985 3.1:+89982765 MS.gene014442:CDS
TCAAAGACATTATCATAAAA+AGG 0.296087 3.1:-89982921 None:intergenic
TTAGTAACAAGACTGTTAAT+TGG 0.301382 3.1:+89982562 MS.gene014442:CDS
TTATGATAATGTCTTTGATT+TGG 0.302543 3.1:+89982926 MS.gene014442:CDS
TAACCCTCATAAACTTCCTT+TGG 0.314405 3.1:+89982290 MS.gene014442:CDS
TTGGGTAAAGATTCCAAAAC+TGG 0.315442 3.1:+89982309 MS.gene014442:CDS
TGCGGGCTGGTGGTTTGTTT+TGG 0.317509 3.1:+89983033 MS.gene014442:CDS
TTAACAGTCTTGTTACTAAC+AGG 0.318712 3.1:-89982558 None:intergenic
TCACTTGTTTACACCCTAAC+AGG 0.330154 3.1:-89983206 None:intergenic
TTGGATACAGAAAATTGAAA+TGG 0.350308 3.1:+89983120 MS.gene014442:CDS
ATAGGATTTGATATTGGAAT+TGG 0.350657 3.1:+89982759 MS.gene014442:CDS
ATTAGAATAGGATTTGATAT+TGG 0.361463 3.1:+89982753 MS.gene014442:CDS
TATATGAATTACAAAGTGTT+TGG 0.369620 3.1:+89982396 MS.gene014442:CDS
TTGCATGCATTGCTTTGATT+TGG 0.380204 3.1:+89982641 MS.gene014442:CDS
AACCCTCATAAACTTCCTTT+GGG 0.381617 3.1:+89982291 MS.gene014442:CDS
CAAAGTGTTTGGAAATTGTC+AGG 0.382386 3.1:+89982407 MS.gene014442:CDS
TTGGAAATTGTCAGGATGAT+TGG 0.389503 3.1:+89982415 MS.gene014442:CDS
ACAAGGTTGATTGAAAGGTT+TGG 0.397211 3.1:+89983101 MS.gene014442:CDS
GGAAATGGTGGAATTAGAAT+AGG 0.400305 3.1:+89982741 MS.gene014442:CDS
CATTGCTTTGATTTGGTGAA+TGG 0.407664 3.1:+89982648 MS.gene014442:CDS
CAAAACTGGAATCACTGAAA+TGG 0.411701 3.1:+89982323 MS.gene014442:CDS
TTACCCAAAGGAAGTTTATG+AGG 0.415254 3.1:-89982294 None:intergenic
TGGATACAGAAAATTGAAAT+GGG 0.423072 3.1:+89983121 MS.gene014442:CDS
CAGAAAATTGAAATGGGTTG+TGG 0.423087 3.1:+89983127 MS.gene014442:CDS
TTCCAAAGTGAATTTGGAAA+AGG 0.423351 3.1:-89982534 None:intergenic
CATTGACAAGGTTGATTGAA+AGG 0.426565 3.1:+89983096 MS.gene014442:CDS
CTTGGTGAAGTTGTTCGGTT+TGG 0.435606 3.1:-89982107 None:intergenic
TGGTAAGAAACAAGAGAAGT+TGG 0.449209 3.1:+89982980 MS.gene014442:CDS
TGTTCTTCAGAAGCCTGTTA+GGG 0.451195 3.1:+89983193 MS.gene014442:CDS
AGAAAATTGAAATGGGTTGT+GGG 0.464176 3.1:+89983128 MS.gene014442:CDS
AATGGTAAGAAATTGAATAG+AGG 0.470921 3.1:+89982618 MS.gene014442:CDS
GTGTACTTGGTGAAGTTGTT+CGG 0.472086 3.1:-89982112 None:intergenic
TGATCCAAGCTTAAGAAAAG+AGG 0.499670 3.1:-89982891 None:intergenic
CACTGAAATGGTTTCATCTG+TGG 0.507607 3.1:+89982335 MS.gene014442:CDS
TGAATGGTTATGAGAATCAA+AGG 0.511808 3.1:+89982664 MS.gene014442:CDS
GCAGCTATAAATTCATTGAA+AGG 0.519697 3.1:-89982855 None:intergenic
GGTTCGTGCTACAAATACCT+TGG 0.520674 3.1:+89982947 MS.gene014442:CDS
AACAAGACTGTTAATTGGAG+TGG 0.522922 3.1:+89982567 MS.gene014442:CDS
GTTTCTTTGAAAATTGGTGA+TGG 0.525785 3.1:+89981964 MS.gene014442:CDS
AAACACCTTCTTCTAGGTAA+AGG 0.526592 3.1:-89982474 None:intergenic
GAAATGGGTTGTGGGAGAAA+AGG 0.531934 3.1:+89983136 MS.gene014442:CDS
GTGGGAGAAAAGGTTGATAG+TGG 0.534948 3.1:+89983146 MS.gene014442:CDS
AATGAATTTATAGCTGCAAG+AGG 0.548634 3.1:+89982861 MS.gene014442:CDS
ACTGAAATGGTTTCATCTGT+GGG 0.553481 3.1:+89982336 MS.gene014442:CDS
TACCCAAAGGAAGTTTATGA+GGG 0.566733 3.1:-89982293 None:intergenic
GTGAGCCTTTACCTAGAAGA+AGG 0.567293 3.1:+89982469 MS.gene014442:CDS
TTGCTGCTGTAATGGCTGAA+AGG 0.580054 3.1:+89982793 MS.gene014442:CDS
AGAAGAAAATTTCATTGACA+AGG 0.582193 3.1:+89983084 MS.gene014442:CDS
CTTACCAACATCATCATCCA+AGG 0.591615 3.1:-89982964 None:intergenic
AATACCTTGGATGATGATGT+TGG 0.652086 3.1:+89982960 MS.gene014442:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAATAGGATTTGATAT+TGG + chr3.1:89982753-89982772 MS.gene014442:CDS 20.0%
!! TCAAAGACATTATCATAAAA+AGG - chr3.1:89982924-89982943 None:intergenic 20.0%
!!! TATATGAATTACAAAGTGTT+TGG + chr3.1:89982396-89982415 MS.gene014442:CDS 20.0%
!!! TTATGATAATGTCTTTGATT+TGG + chr3.1:89982926-89982945 MS.gene014442:CDS 20.0%
! AATGGTAAGAAATTGAATAG+AGG + chr3.1:89982618-89982637 MS.gene014442:CDS 25.0%
! AGAAGAAAATTTCATTGACA+AGG + chr3.1:89983084-89983103 MS.gene014442:CDS 25.0%
! ATAGGATTTGATATTGGAAT+TGG + chr3.1:89982759-89982778 MS.gene014442:CDS 25.0%
! TGGATACAGAAAATTGAAAT+GGG + chr3.1:89983121-89983140 MS.gene014442:CDS 25.0%
! TTAGTAACAAGACTGTTAAT+TGG + chr3.1:89982562-89982581 MS.gene014442:CDS 25.0%
! TTGGATACAGAAAATTGAAA+TGG + chr3.1:89983120-89983139 MS.gene014442:CDS 25.0%
!! AAAAAGATTGGTTGTGATAA+CGG - chr3.1:89982052-89982071 None:intergenic 25.0%
!!! AAGAATTTTGAGTGTTTGAA+TGG + chr3.1:89982600-89982619 MS.gene014442:CDS 25.0%
!!! CTCAGAAATTGATTTTGAAA+GGG + chr3.1:89982445-89982464 MS.gene014442:CDS 25.0%
AATGAATTTATAGCTGCAAG+AGG + chr3.1:89982861-89982880 MS.gene014442:CDS 30.0%
AGCATAAAGAGCAAAAAGAT+TGG - chr3.1:89982064-89982083 None:intergenic 30.0%
GCAGCTATAAATTCATTGAA+AGG - chr3.1:89982858-89982877 None:intergenic 30.0%
TGAATGGTTATGAGAATCAA+AGG + chr3.1:89982664-89982683 MS.gene014442:CDS 30.0%
TTCCAAAGTGAATTTGGAAA+AGG - chr3.1:89982537-89982556 None:intergenic 30.0%
! ATCCTTTTCCAAATTCACTT+TGG + chr3.1:89982532-89982551 MS.gene014442:CDS 30.0%
! GGTTCTGTTTCTTTGAAAAT+TGG + chr3.1:89981958-89981977 MS.gene014442:CDS 30.0%
! GTTTCTTTGAAAATTGGTGA+TGG + chr3.1:89981964-89981983 MS.gene014442:CDS 30.0%
! TGCTAAATCTGTTTCTTCTA+AGG + chr3.1:89982497-89982516 MS.gene014442:CDS 30.0%
! TTAAGCTCTTTTGCAATGTT+GGG - chr3.1:89982261-89982280 None:intergenic 30.0%
! TTTAAGCTCTTTTGCAATGT+TGG - chr3.1:89982262-89982281 None:intergenic 30.0%
! TTTGATATTGGAATTGGTTC+TGG + chr3.1:89982765-89982784 MS.gene014442:CDS 30.0%
! TTTTCCTCTTTTCTTAAGCT+TGG + chr3.1:89982887-89982906 MS.gene014442:CDS 30.0%
! TTTTCTTAAGCTTGGATCAT+AGG + chr3.1:89982895-89982914 MS.gene014442:CDS 30.0%
!! AGAAAATTGAAATGGGTTGT+GGG + chr3.1:89983128-89983147 MS.gene014442:CDS 30.0%
!! GCTCAGAAATTGATTTTGAA+AGG + chr3.1:89982444-89982463 MS.gene014442:CDS 30.0%
!! TTAACAGTCTTGTTACTAAC+AGG - chr3.1:89982561-89982580 None:intergenic 30.0%
!! TTTTCTTGCTGATGATGTTT+TGG + chr3.1:89982713-89982732 MS.gene014442:CDS 30.0%
!!! AAGAAGCTGTTTTTCTATCT+GGG - chr3.1:89982211-89982230 None:intergenic 30.0%
AAACACCTTCTTCTAGGTAA+AGG - chr3.1:89982477-89982496 None:intergenic 35.0%
AACAAGACTGTTAATTGGAG+TGG + chr3.1:89982567-89982586 MS.gene014442:CDS 35.0%
AACCCTCATAAACTTCCTTT+GGG + chr3.1:89982291-89982310 MS.gene014442:CDS 35.0%
ACAGGTTTCCAAAGTGAATT+TGG - chr3.1:89982543-89982562 None:intergenic 35.0%
ACTGAAATGGTTTCATCTGT+GGG + chr3.1:89982336-89982355 MS.gene014442:CDS 35.0%
CAAAACTGGAATCACTGAAA+TGG + chr3.1:89982323-89982342 MS.gene014442:CDS 35.0%
CAGATAGAAAAACAGCTTCT+TGG + chr3.1:89982210-89982229 MS.gene014442:CDS 35.0%
TAACCCTCATAAACTTCCTT+TGG + chr3.1:89982290-89982309 MS.gene014442:CDS 35.0%
TACCCAAAGGAAGTTTATGA+GGG - chr3.1:89982296-89982315 None:intergenic 35.0%
TGATCCAAGCTTAAGAAAAG+AGG - chr3.1:89982894-89982913 None:intergenic 35.0%
TGGTAAGAAACAAGAGAAGT+TGG + chr3.1:89982980-89982999 MS.gene014442:CDS 35.0%
TTACCCAAAGGAAGTTTATG+AGG - chr3.1:89982297-89982316 None:intergenic 35.0%
TTAGCAAAACACCTTCTTCT+AGG - chr3.1:89982483-89982502 None:intergenic 35.0%
TTGCATGCATTGCTTTGATT+TGG + chr3.1:89982641-89982660 MS.gene014442:CDS 35.0%
TTGGAAATTGTCAGGATGAT+TGG + chr3.1:89982415-89982434 MS.gene014442:CDS 35.0%
TTGGGTAAAGATTCCAAAAC+TGG + chr3.1:89982309-89982328 MS.gene014442:CDS 35.0%
! AATACCTTGGATGATGATGT+TGG + chr3.1:89982960-89982979 MS.gene014442:CDS 35.0%
! ACAAGGTTGATTGAAAGGTT+TGG + chr3.1:89983101-89983120 MS.gene014442:CDS 35.0%
! CAAAGTGTTTGGAAATTGTC+AGG + chr3.1:89982407-89982426 MS.gene014442:CDS 35.0%
! CATTGACAAGGTTGATTGAA+AGG + chr3.1:89983096-89983115 MS.gene014442:CDS 35.0%
! CATTTCAGTGATTCCAGTTT+TGG - chr3.1:89982325-89982344 None:intergenic 35.0%
! GAGAGAAGTTTTTGTGTACT+TGG - chr3.1:89982128-89982147 None:intergenic 35.0%
! GGAAATGGTGGAATTAGAAT+AGG + chr3.1:89982741-89982760 MS.gene014442:CDS 35.0%
!! CAGAAAATTGAAATGGGTTG+TGG + chr3.1:89983127-89983146 MS.gene014442:CDS 35.0%
!! CATTGCTTTGATTTGGTGAA+TGG + chr3.1:89982648-89982667 MS.gene014442:CDS 35.0%
!!! CAAGAAGCTGTTTTTCTATC+TGG - chr3.1:89982212-89982231 None:intergenic 35.0%
!!! GATGTTTTGGCATTAGGAAA+TGG + chr3.1:89982726-89982745 MS.gene014442:CDS 35.0%
!!! GTTTTGGAATCTTTACCCAA+AGG - chr3.1:89982309-89982328 None:intergenic 35.0%
CACTGAAATGGTTTCATCTG+TGG + chr3.1:89982335-89982354 MS.gene014442:CDS 40.0%
CTTACCAACATCATCATCCA+AGG - chr3.1:89982967-89982986 None:intergenic 40.0%
TGTTCTTCAGAAGCCTGTTA+GGG + chr3.1:89983193-89983212 MS.gene014442:CDS 40.0%
! GTGTACTTGGTGAAGTTGTT+CGG - chr3.1:89982115-89982134 None:intergenic 40.0%
! TCGACATCGATCGAATTTTG+CGG + chr3.1:89983015-89983034 MS.gene014442:CDS 40.0%
! TGGATCTTTTGCTGCTGTAA+TGG + chr3.1:89982785-89982804 MS.gene014442:CDS 40.0%
!! GTTTTGGCATTAGGAAATGG+TGG + chr3.1:89982729-89982748 MS.gene014442:CDS 40.0%
!!! GCTGATGATGTTTTGGCATT+AGG + chr3.1:89982720-89982739 MS.gene014442:CDS 40.0%
!!! TGTTCGGTTTGGCTGTTTTT+TGG - chr3.1:89982099-89982118 None:intergenic 40.0%
CTGTTCTTCAGAAGCCTGTT+AGG + chr3.1:89983192-89983211 MS.gene014442:CDS 45.0%
CTTGGTGAAGTTGTTCGGTT+TGG - chr3.1:89982110-89982129 None:intergenic 45.0%
GGTTCGTGCTACAAATACCT+TGG + chr3.1:89982947-89982966 MS.gene014442:CDS 45.0%
GTGAGCCTTTACCTAGAAGA+AGG + chr3.1:89982469-89982488 MS.gene014442:CDS 45.0%
TTGCTGCTGTAATGGCTGAA+AGG + chr3.1:89982793-89982812 MS.gene014442:CDS 45.0%
! CGACATCGATCGAATTTTGC+GGG + chr3.1:89983016-89983035 MS.gene014442:CDS 45.0%
!! GAAATGGGTTGTGGGAGAAA+AGG + chr3.1:89983136-89983155 MS.gene014442:CDS 45.0%
!! GTGGGAGAAAAGGTTGATAG+TGG + chr3.1:89983146-89983165 MS.gene014442:CDS 45.0%
! ATCGATCGAATTTTGCGGGC+TGG + chr3.1:89983020-89983039 MS.gene014442:CDS 50.0%
!!! GATCGAATTTTGCGGGCTGG+TGG + chr3.1:89983023-89983042 MS.gene014442:CDS 55.0%
!!! TGCGGGCTGGTGGTTTGTTT+TGG + chr3.1:89983033-89983052 MS.gene014442:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 89981955 89983220 89981955 ID=MS.gene014442
chr3.1 mRNA 89981955 89983220 89981955 ID=MS.gene014442.t1;Parent=MS.gene014442
chr3.1 exon 89981955 89983220 89981955 ID=MS.gene014442.t1.exon1;Parent=MS.gene014442.t1
chr3.1 CDS 89981955 89983220 89981955 ID=cds.MS.gene014442.t1;Parent=MS.gene014442.t1
Gene Sequence

>MS.gene014442

ATGGGTTCTGTTTCTTTGAAAATTGGTGATGGAACAGCAAGATTCAAAAAATCAACACTTTGTTCTTCAGCTGTTAACATTCTCATGATCTTATCCGTTATCACAACCAATCTTTTTGCTCTTTATGCTTTCACTTCTTCTCCAAAAAACAGCCAAACCGAACAACTTCACCAAGTACACAAAAACTTCTCTCTCATCTCAGAACAAGTTTCACTTATACTCAGAGAAATTGATCAATCTCAAAAGAAACTAGCCCAGATAGAAAAACAGCTTCTTGGCTATGAAAGTTTTGATCTTTCAAGACCCAACATTGCAAAAGAGCTTAAACTCTTTCTTAACCCTCATAAACTTCCTTTGGGTAAAGATTCCAAAACTGGAATCACTGAAATGGTTTCATCTGTGGGTCATTCTTGTGAGAAATCTTCTGATTTGTTGTCTCAGTATATGAATTACAAAGTGTTTGGAAATTGTCAGGATGATTGGAGTTATGCTCAGAAATTGATTTTGAAAGGGTGTGAGCCTTTACCTAGAAGAAGGTGTTTTGCTAAATCTGTTTCTTCTAAGGTAAATTATCTTCATCCTTTTCCAAATTCACTTTGGAAACCTGTTAGTAACAAGACTGTTAATTGGAGTGGTTTTAGTTGTAAGAATTTTGAGTGTTTGAATGGTAAGAAATTGAATAGAGGTTGCATGCATTGCTTTGATTTGGTGAATGGTTATGAGAATCAAAGGTTTGTTAAGTCTAGAAGTAAGAATGATTTTCTTGCTGATGATGTTTTGGCATTAGGAAATGGTGGAATTAGAATAGGATTTGATATTGGAATTGGTTCTGGATCTTTTGCTGCTGTAATGGCTGAAAGGAATGTTACAATTGTTACTAGTACTCTTAATATTGATGCTCCTTTCAATGAATTTATAGCTGCAAGAGGACTTTTTCCTCTTTTCTTAAGCTTGGATCATAGGTTTCCTTTTTATGATAATGTCTTTGATTTGGTTCGTGCTACAAATACCTTGGATGATGATGTTGGTAAGAAACAAGAGAAGTTGGAGTTTTTAATGTTCGACATCGATCGAATTTTGCGGGCTGGTGGTTTGTTTTGGATTGATAACTTCTATTGTGCTAATGATGAGAAGAAAATTTCATTGACAAGGTTGATTGAAAGGTTTGGATACAGAAAATTGAAATGGGTTGTGGGAGAAAAGGTTGATAGTGGTAAGTCTCAAGTTTTCTTATCTGCTGTTCTTCAGAAGCCTGTTAGGGTGTAA

Protein sequence

>MS.gene014442.t1

MGSVSLKIGDGTARFKKSTLCSSAVNILMILSVITTNLFALYAFTSSPKNSQTEQLHQVHKNFSLISEQVSLILREIDQSQKKLAQIEKQLLGYESFDLSRPNIAKELKLFLNPHKLPLGKDSKTGITEMVSSVGHSCEKSSDLLSQYMNYKVFGNCQDDWSYAQKLILKGCEPLPRRRCFAKSVSSKVNYLHPFPNSLWKPVSNKTVNWSGFSCKNFECLNGKKLNRGCMHCFDLVNGYENQRFVKSRSKNDFLADDVLALGNGGIRIGFDIGIGSGSFAAVMAERNVTIVTSTLNIDAPFNEFIAARGLFPLFLSLDHRFPFYDNVFDLVRATNTLDDDVGKKQEKLEFLMFDIDRILRAGGLFWIDNFYCANDEKKISLTRLIERFGYRKLKWVVGEKVDSGKSQVFLSAVLQKPVRV