AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24186


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24186.t1 MTR_1g024005 97.887 426 9 0 1 426 1 426 0.0 856
MS.gene24186.t1 MTR_3g113270 75.758 429 91 5 1 425 1 420 0.0 655
MS.gene24186.t1 MTR_3g071050 72.694 271 66 3 29 297 1 265 2.05e-139 400
MS.gene24186.t1 MTR_2g028590 44.720 322 169 5 109 424 145 463 1.42e-91 284
MS.gene24186.t1 MTR_2g028610 37.063 429 232 9 30 424 34 458 4.97e-91 283
MS.gene24186.t1 MTR_5g077710 43.810 315 163 7 120 425 76 385 3.05e-89 276
MS.gene24186.t1 MTR_8g028460 41.088 331 174 7 109 425 164 487 2.81e-80 256
MS.gene24186.t1 MTR_0010s0400 39.198 324 180 7 109 426 147 459 1.32e-77 248
MS.gene24186.t1 MTR_3g070620 76.471 136 29 2 228 361 54 188 2.48e-67 213
MS.gene24186.t1 MTR_4g094572 33.012 415 226 8 19 424 4 375 2.87e-64 211
MS.gene24186.t1 MTR_3g071100 68.293 41 12 1 208 248 24 63 1.09e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24186.t1 AT3G27230 72.300 426 102 3 1 426 1 410 0.0 629
MS.gene24186.t1 AT5G40830 69.393 428 113 6 1 425 1 413 0.0 607
MS.gene24186.t1 AT5G40830 69.393 428 113 6 1 425 1 413 0.0 607
MS.gene24186.t1 AT5G40830 63.084 428 100 6 1 425 1 373 0.0 537
MS.gene24186.t1 AT3G05390 32.969 458 242 11 23 426 16 462 2.15e-79 253
MS.gene24186.t1 AT4G01240 40.000 330 178 7 109 425 334 656 7.23e-75 246
MS.gene24186.t1 AT1G29790 33.416 404 232 9 24 424 7 376 1.85e-69 224
MS.gene24186.t1 AT1G29790 33.416 404 232 9 24 424 7 376 1.85e-69 224

Find 60 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCAGAAACTGATCTTAAA+AGG 0.211099 1.2:-1337321 MS.gene24186:CDS
TCAAACACATTGTCATAAAA+TGG 0.248817 1.2:+1336847 None:intergenic
AGAAACCAGTTAGGGGTTAA+AGG 0.250275 1.2:-1336564 None:intergenic
GGTTCAGTTTCACTGAAAAT+TGG 0.255151 1.2:-1337822 MS.gene24186:CDS
GGAGAGAAAGTTGATAGTTT+TGG 0.265881 1.2:-1336625 MS.gene24186:CDS
TTTGATATTGGTGGTGGTTC+AGG 0.273729 1.2:-1337003 MS.gene24186:CDS
CTAAAGGAAGTTGATGTCTT+TGG 0.285958 1.2:+1337475 None:intergenic
CTGGTGCTTGTCCTGATGAT+TGG 0.293180 1.2:-1337350 MS.gene24186:CDS
TGCGGGCCGGTGGTTTGTTT+TGG 0.299687 1.2:-1336741 MS.gene24186:CDS
CAAAGACATCAACTTCCTTT+AGG 0.332526 1.2:-1337474 MS.gene24186:CDS
TTTACTTGAGCTTAGATCAT+AGG 0.343285 1.2:-1336873 MS.gene24186:CDS
GCAGCAATGAATTCACTAAA+TGG 0.344054 1.2:+1336913 None:intergenic
GGAAATGGTGGAATTAGAAT+AGG 0.346150 1.2:-1337027 MS.gene24186:CDS
CTGTTCTTCAGAAACCAGTT+AGG 0.347883 1.2:-1336573 MS.gene24186:CDS
ACAAAGTTGATTGAAAGGTT+TGG 0.356071 1.2:-1336673 MS.gene24186:CDS
TTCAGCTCATTTGCAATGTT+GGG 0.356522 1.2:+1337507 None:intergenic
TTCATGAGTTACAAGGCTTC+TGG 0.364603 1.2:-1337369 MS.gene24186:CDS
CCTAACCTTTAACCCCTAAC+TGG 0.366164 1.2:+1336559 None:intergenic
ATTAGAATAGGGTTTGATAT+TGG 0.369699 1.2:-1337015 MS.gene24186:CDS
GAAATGGTGGAATTAGAATA+GGG 0.380459 1.2:-1337026 MS.gene24186:CDS
TTGGATATAAGAAATTGAAA+TGG 0.387064 1.2:-1336654 MS.gene24186:CDS
TTAGGCAAGGATTCAAGAAC+TGG 0.392044 1.2:-1337456 MS.gene24186:CDS
TTTCAGCTCATTTGCAATGT+TGG 0.396349 1.2:+1337506 None:intergenic
TGGATATAAGAAATTGAAAT+GGG 0.396901 1.2:-1336653 MS.gene24186:CDS
ACTGTTGTTGTTGGTGGTTG+TGG 0.402189 1.2:+1337668 None:intergenic
AGAGGCTTCCAAAGAGAAAC+AGG 0.426242 1.2:+1337231 None:intergenic
GTTTCTCTTTGGAAGCCTCT+TGG 0.431268 1.2:-1337228 MS.gene24186:CDS
AAGGTTACTGTTGTTGTTGG+TGG 0.444379 1.2:+1337662 None:intergenic
TAAGAAATTGAAATGGGTTG+TGG 0.451047 1.2:-1336647 MS.gene24186:CDS
ATTAAGGTTACTGTTGTTGT+TGG 0.452349 1.2:+1337659 None:intergenic
AAGAAATTGAAATGGGTTGT+GGG 0.464176 1.2:-1336646 MS.gene24186:CDS
TGTTCTTCAGAAACCAGTTA+GGG 0.468058 1.2:-1336572 MS.gene24186:CDS
AAGGAAGTTGATGTCTTTGG+AGG 0.474654 1.2:+1337478 None:intergenic
AAACACCTTCTTCTTGGTAA+AGG 0.474985 1.2:+1337291 None:intergenic
GTTCTTGAATCCTTGCCTAA+AGG 0.475247 1.2:+1337459 None:intergenic
CTCTAACAAAGTTGATTGAA+AGG 0.480902 1.2:-1336678 MS.gene24186:CDS
CTCAGAAACTGATCTTAAAA+GGG 0.485828 1.2:-1337320 MS.gene24186:CDS
TGGAGGAAAAGCTGAAAAGT+TGG 0.486068 1.2:-1336794 MS.gene24186:CDS
AGAATAGGGTTTGATATTGG+TGG 0.487533 1.2:-1337012 MS.gene24186:CDS
AAGAACTGGGATCACTGAAA+TGG 0.496871 1.2:-1337442 MS.gene24186:CDS
TGATCTAAGCTCAAGTAAAG+TGG 0.497485 1.2:+1336877 None:intergenic
ATAGGGTTTGATATTGGTGG+TGG 0.514507 1.2:-1337009 MS.gene24186:CDS
TGAGCTACACTCCAATCATC+AGG 0.519786 1.2:+1337339 None:intergenic
GATTCATGCATCAAGTGCAT+TGG 0.520145 1.2:-1336821 MS.gene24186:CDS
TCAAAAGAAACTAGCCCAGA+TGG 0.525921 1.2:-1337571 MS.gene24186:CDS
GTTTCACTGAAAATTGGAGA+TGG 0.532703 1.2:-1337816 MS.gene24186:CDS
GTCACAGTTCATGAGTTACA+AGG 0.534645 1.2:-1337376 MS.gene24186:CDS
TTCCAAAGAGAAACAGGAAA+AGG 0.536142 1.2:+1337237 None:intergenic
ACATCAACTTCCTTTAGGCA+AGG 0.546386 1.2:-1337469 MS.gene24186:CDS
AGTGAATTCATTGCTGCTAG+AGG 0.555907 1.2:-1336907 MS.gene24186:CDS
GCATCAAGTGCATTGGATGT+TGG 0.560273 1.2:-1336814 MS.gene24186:CDS
GTGAGCCTTTACCAAGAAGA+AGG 0.569775 1.2:-1337296 MS.gene24186:CDS
ACTGAAATGGTGTCATCAGT+TGG 0.572185 1.2:-1337429 MS.gene24186:CDS
TTAAAAGTGTTATTACCAAG+AGG 0.586025 1.2:+1337213 None:intergenic
TGTTGTTGGTGGTTGTGGTG+TGG 0.595503 1.2:+1337673 None:intergenic
TGAGAATCAGAGATTTGTGA+AGG 0.606302 1.2:-1337094 MS.gene24186:CDS
ATCAAACCAAAACAAACCAC+CGG 0.608513 1.2:+1336735 None:intergenic
TCAAGTGCATTGGATGTTGG+AGG 0.648963 1.2:-1336811 MS.gene24186:CDS
TAGGCAAGGATTCAAGAACT+GGG 0.665439 1.2:-1337455 MS.gene24186:CDS
GTTCTTCAGAAACCAGTTAG+GGG 0.683618 1.2:-1336571 MS.gene24186:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAGTAAAAATGATTTTT+TGG - chr1.2:1337322-1337341 MS.gene24186:CDS 10.0%
!! TGGATATAAGAAATTGAAAT+GGG - chr1.2:1337739-1337758 MS.gene24186:CDS 20.0%
!! TTGGATATAAGAAATTGAAA+TGG - chr1.2:1337738-1337757 MS.gene24186:CDS 20.0%
!!! TTGGTAATAACACTTTTAAT+TGG - chr1.2:1337183-1337202 MS.gene24186:CDS 20.0%
!!! TTGTGTTAATTGTTTTGATT+TGG - chr1.2:1337262-1337281 MS.gene24186:CDS 20.0%
! ATTAGAATAGGGTTTGATAT+TGG - chr1.2:1337377-1337396 MS.gene24186:CDS 25.0%
! TCAAAAACTGTTTCAAAATC+AGG - chr1.2:1337125-1337144 MS.gene24186:CDS 25.0%
! TCAAACACATTGTCATAAAA+TGG + chr1.2:1337548-1337567 None:intergenic 25.0%
!!! AAGAGTTTTGAATGTTTGAA+AGG - chr1.2:1337221-1337240 MS.gene24186:CDS 25.0%
!!! AATAACACTTTTAATTGGAG+TGG - chr1.2:1337188-1337207 MS.gene24186:CDS 25.0%
!!! AATTGTTTTGATTTGGTTAG+TGG - chr1.2:1337269-1337288 MS.gene24186:CDS 25.0%
!!! AGATGTTTTTGTGAAGATTA+AGG + chr1.2:1336752-1336771 None:intergenic 25.0%
!!! TTAAAAGTGTTATTACCAAG+AGG + chr1.2:1337182-1337201 None:intergenic 25.0%
ATTAAGGTTACTGTTGTTGT+TGG + chr1.2:1336736-1336755 None:intergenic 30.0%
CTCAGAAACTGATCTTAAAA+GGG - chr1.2:1337072-1337091 MS.gene24186:CDS 30.0%
TTTACTTGAGCTTAGATCAT+AGG - chr1.2:1337519-1337538 MS.gene24186:CDS 30.0%
TTTGAAAAACACCTTCTTCT+TGG + chr1.2:1337110-1337129 None:intergenic 30.0%
! ACAAAGTTGATTGAAAGGTT+TGG - chr1.2:1337719-1337738 MS.gene24186:CDS 30.0%
! CTCTAACAAAGTTGATTGAA+AGG - chr1.2:1337714-1337733 MS.gene24186:CDS 30.0%
! GAAATGGTGGAATTAGAATA+GGG - chr1.2:1337366-1337385 MS.gene24186:CDS 30.0%
!! AAGAAATTGAAATGGGTTGT+GGG - chr1.2:1337746-1337765 MS.gene24186:CDS 30.0%
!! TAAGAAATTGAAATGGGTTG+TGG - chr1.2:1337745-1337764 MS.gene24186:CDS 30.0%
!!! AAAGTTGATAGTTTTGGATC+AGG - chr1.2:1337773-1337792 MS.gene24186:CDS 30.0%
!!! GATGTTTTAGCATTAGGAAA+TGG - chr1.2:1337350-1337369 MS.gene24186:CDS 30.0%
!!! GTTGATGATGTTTTAGCATT+AGG - chr1.2:1337344-1337363 MS.gene24186:CDS 30.0%
AAACACCTTCTTCTTGGTAA+AGG + chr1.2:1337104-1337123 None:intergenic 35.0%
AGAATAGGGTTTGATATTGG+TGG - chr1.2:1337380-1337399 MS.gene24186:CDS 35.0%
ATCAAACCAAAACAAACCAC+CGG + chr1.2:1337660-1337679 None:intergenic 35.0%
CAAAGACATCAACTTCCTTT+AGG - chr1.2:1336918-1336937 MS.gene24186:CDS 35.0%
GCAGCAATGAATTCACTAAA+TGG + chr1.2:1337482-1337501 None:intergenic 35.0%
GCTCAGAAACTGATCTTAAA+AGG - chr1.2:1337071-1337090 MS.gene24186:CDS 35.0%
GGTTCAGTTTCACTGAAAAT+TGG - chr1.2:1336570-1336589 MS.gene24186:CDS 35.0%
GTTTCACTGAAAATTGGAGA+TGG - chr1.2:1336576-1336595 MS.gene24186:CDS 35.0%
TGAGAATCAGAGATTTGTGA+AGG - chr1.2:1337298-1337317 MS.gene24186:CDS 35.0%
TGATCTAAGCTCAAGTAAAG+TGG + chr1.2:1337518-1337537 None:intergenic 35.0%
TGTTCTTCAGAAACCAGTTA+GGG - chr1.2:1337820-1337839 MS.gene24186:CDS 35.0%
TTCAGCTCATTTGCAATGTT+GGG + chr1.2:1336888-1336907 None:intergenic 35.0%
TTCCAAAGAGAAACAGGAAA+AGG + chr1.2:1337158-1337177 None:intergenic 35.0%
TTTCAGCTCATTTGCAATGT+TGG + chr1.2:1336889-1336908 None:intergenic 35.0%
! GGAAATGGTGGAATTAGAAT+AGG - chr1.2:1337365-1337384 MS.gene24186:CDS 35.0%
! TTGATATCGATCGAGTTTTG+CGG - chr1.2:1337633-1337652 MS.gene24186:CDS 35.0%
!! CTAAAGGAAGTTGATGTCTT+TGG + chr1.2:1336920-1336939 None:intergenic 35.0%
!! GTTTTAGCATTAGGAAATGG+TGG - chr1.2:1337353-1337372 MS.gene24186:CDS 35.0%
!! TTCCTTTTCCTGTTTCTCTT+TGG - chr1.2:1337153-1337172 MS.gene24186:CDS 35.0%
!!! AAGAAGCTGTTTTTCCATCT+GGG + chr1.2:1336838-1336857 None:intergenic 35.0%
!!! GGAGAGAAAGTTGATAGTTT+TGG - chr1.2:1337767-1337786 MS.gene24186:CDS 35.0%
!!! TGTTTTGATTTGGTTAGTGG+TGG - chr1.2:1337272-1337291 MS.gene24186:CDS 35.0%
AAGAACTGGGATCACTGAAA+TGG - chr1.2:1336950-1336969 MS.gene24186:CDS 40.0%
ACATCAACTTCCTTTAGGCA+AGG - chr1.2:1336923-1336942 MS.gene24186:CDS 40.0%
AGTGAATTCATTGCTGCTAG+AGG - chr1.2:1337485-1337504 MS.gene24186:CDS 40.0%
ATAGGGTTTGATATTGGTGG+TGG - chr1.2:1337383-1337402 MS.gene24186:CDS 40.0%
CAGATGGAAAAACAGCTTCT+TGG - chr1.2:1336837-1336856 MS.gene24186:CDS 40.0%
CTGTTCTTCAGAAACCAGTT+AGG - chr1.2:1337819-1337838 MS.gene24186:CDS 40.0%
GATTCATGCATCAAGTGCAT+TGG - chr1.2:1337571-1337590 MS.gene24186:CDS 40.0%
GTCACAGTTCATGAGTTACA+AGG - chr1.2:1337016-1337035 MS.gene24186:CDS 40.0%
GTTCTTCAGAAACCAGTTAG+GGG - chr1.2:1337821-1337840 MS.gene24186:CDS 40.0%
GTTCTTGAATCCTTGCCTAA+AGG + chr1.2:1336936-1336955 None:intergenic 40.0%
TAGGCAAGGATTCAAGAACT+GGG - chr1.2:1336937-1336956 MS.gene24186:CDS 40.0%
TCAAAAGAAACTAGCCCAGA+TGG - chr1.2:1336821-1336840 MS.gene24186:CDS 40.0%
TGGAGGAAAAGCTGAAAAGT+TGG - chr1.2:1337598-1337617 MS.gene24186:CDS 40.0%
TTAGGCAAGGATTCAAGAAC+TGG - chr1.2:1336936-1336955 MS.gene24186:CDS 40.0%
TTCATGAGTTACAAGGCTTC+TGG - chr1.2:1337023-1337042 MS.gene24186:CDS 40.0%
! AAGGTTACTGTTGTTGTTGG+TGG + chr1.2:1336733-1336752 None:intergenic 40.0%
! TGATATCGATCGAGTTTTGC+GGG - chr1.2:1337634-1337653 MS.gene24186:CDS 40.0%
!! AAGGAAGTTGATGTCTTTGG+AGG + chr1.2:1336917-1336936 None:intergenic 40.0%
!! ACTGAAATGGTGTCATCAGT+TGG - chr1.2:1336963-1336982 MS.gene24186:CDS 40.0%
!! TTTGATATTGGTGGTGGTTC+AGG - chr1.2:1337389-1337408 MS.gene24186:CDS 40.0%
!!! CAAGAAGCTGTTTTTCCATC+TGG + chr1.2:1336839-1336858 None:intergenic 40.0%
AGAGGCTTCCAAAGAGAAAC+AGG + chr1.2:1337164-1337183 None:intergenic 45.0%
GCATCAAGTGCATTGGATGT+TGG - chr1.2:1337578-1337597 MS.gene24186:CDS 45.0%
GTGAGCCTTTACCAAGAAGA+AGG - chr1.2:1337096-1337115 MS.gene24186:CDS 45.0%
TCAAGTGCATTGGATGTTGG+AGG - chr1.2:1337581-1337600 MS.gene24186:CDS 45.0%
TGAGCTACACTCCAATCATC+AGG + chr1.2:1337056-1337075 None:intergenic 45.0%
! GTTTCTCTTTGGAAGCCTCT+TGG - chr1.2:1337164-1337183 MS.gene24186:CDS 45.0%
!! ACTGTTGTTGTTGGTGGTTG+TGG + chr1.2:1336727-1336746 None:intergenic 45.0%
CTGGTGCTTGTCCTGATGAT+TGG - chr1.2:1337042-1337061 MS.gene24186:CDS 50.0%
! GGTTGTGGTGTGGCTTTTTG+AGG + chr1.2:1336712-1336731 None:intergenic 50.0%
!! TGTTGTTGGTGGTTGTGGTG+TGG + chr1.2:1336722-1336741 None:intergenic 50.0%
! ATCGATCGAGTTTTGCGGGC+CGG - chr1.2:1337638-1337657 MS.gene24186:CDS 55.0%
!!! TGCGGGCCGGTGGTTTGTTT+TGG - chr1.2:1337651-1337670 MS.gene24186:CDS 60.0%
! GATCGAGTTTTGCGGGCCGG+TGG - chr1.2:1337641-1337660 MS.gene24186:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 1336567 1337847 1336567 ID=MS.gene24186
chr1.2 mRNA 1336567 1337847 1336567 ID=MS.gene24186.t1;Parent=MS.gene24186
chr1.2 exon 1336567 1337847 1336567 ID=MS.gene24186.t1.exon1;Parent=MS.gene24186.t1
chr1.2 CDS 1336567 1337847 1336567 ID=cds.MS.gene24186.t1;Parent=MS.gene24186.t1
Gene Sequence

>MS.gene24186

ATGGGTTCAGTTTCACTGAAAATTGGAGATGGAACAGCAAGATTCAAAAGAGCAACACTTTGTTCTTCAGCTGTTAACATTCTCATGATTTTCTGTGTCATCACAACTAATCTCTTTGCTCTCTATGCTTTCACTTCTTCTTCCTCAAAAAGCCACACCACAACCACCAACAACAACAGTAACCTTAATCTTCACAAAAACATCTCTCTTGTCTCAGAACAAGTCTCACTCATACTCAGAGAGATAGATTTGTCTCAAAAGAAACTAGCCCAGATGGAAAAACAGCTTCTTGGTTATGAAAGCATTGATCTTTCACGACCCAACATTGCAAATGAGCTGAAAATCTTCCTCCAAAGACATCAACTTCCTTTAGGCAAGGATTCAAGAACTGGGATCACTGAAATGGTGTCATCAGTTGGTCATTCTTGTGAGAAAAATTCAGACTTGTTGTCACAGTTCATGAGTTACAAGGCTTCTGGTGCTTGTCCTGATGATTGGAGTGTAGCTCAGAAACTGATCTTAAAAGGGTGTGAGCCTTTACCAAGAAGAAGGTGTTTTTCAAAAACTGTTTCAAAATCAGGTTTTTTTCCTTTTCCTGTTTCTCTTTGGAAGCCTCTTGGTAATAACACTTTTAATTGGAGTGGTTTGAATTGTAAGAGTTTTGAATGTTTGAAAGGTAAGAAATTGAGTAGAGATTGTGTTAATTGTTTTGATTTGGTTAGTGGTGGAAATGAGAATCAGAGATTTGTGAAGGCAAAAAGTAAAAATGATTTTTTGGTTGATGATGTTTTAGCATTAGGAAATGGTGGAATTAGAATAGGGTTTGATATTGGTGGTGGTTCAGGTTCTTTTGCTGCTAGAATGTTTGATAGAAATGTTACTGTGATTACGAATACTCTTAATGTTGATGCACCATTTAGTGAATTCATTGCTGCTAGAGGACTTTTTCCACTTTACTTGAGCTTAGATCATAGGTTTCCATTTTATGACAATGTGTTTGATTTGATTCATGCATCAAGTGCATTGGATGTTGGAGGAAAAGCTGAAAAGTTGGAGTTTTTAATGTTTGATATCGATCGAGTTTTGCGGGCCGGTGGTTTGTTTTGGTTTGATAACTTCTTTTGTTTTAGTGAAGAGAAGAAACAAACTCTAACAAAGTTGATTGAAAGGTTTGGATATAAGAAATTGAAATGGGTTGTGGGAGAGAAAGTTGATAGTTTTGGATCAGGAAAATCTGAAGTTGTGTTGTCTGCTGTTCTTCAGAAACCAGTTAGGGGTTAA

Protein sequence

>MS.gene24186.t1

MGSVSLKIGDGTARFKRATLCSSAVNILMIFCVITTNLFALYAFTSSSSKSHTTTTNNNSNLNLHKNISLVSEQVSLILREIDLSQKKLAQMEKQLLGYESIDLSRPNIANELKIFLQRHQLPLGKDSRTGITEMVSSVGHSCEKNSDLLSQFMSYKASGACPDDWSVAQKLILKGCEPLPRRRCFSKTVSKSGFFPFPVSLWKPLGNNTFNWSGLNCKSFECLKGKKLSRDCVNCFDLVSGGNENQRFVKAKSKNDFLVDDVLALGNGGIRIGFDIGGGSGSFAARMFDRNVTVITNTLNVDAPFSEFIAARGLFPLYLSLDHRFPFYDNVFDLIHASSALDVGGKAEKLEFLMFDIDRVLRAGGLFWFDNFFCFSEEKKQTLTKLIERFGYKKLKWVVGEKVDSFGSGKSEVVLSAVLQKPVRG