AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene69420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69420.t1 MTR_3g113270 97.387 421 11 0 1 421 1 421 0.0 848
MS.gene69420.t1 MTR_1g024005 76.457 429 88 5 1 420 1 425 0.0 656
MS.gene69420.t1 MTR_3g071050 97.358 265 7 0 29 293 1 265 0.0 540
MS.gene69420.t1 MTR_3g070620 97.037 135 4 0 225 359 54 188 2.49e-91 274
MS.gene69420.t1 MTR_2g028590 40.053 377 208 8 56 419 92 463 5.20e-89 278
MS.gene69420.t1 MTR_2g028610 37.123 431 224 11 30 419 34 458 9.37e-89 277
MS.gene69420.t1 MTR_5g077710 42.722 316 169 8 113 420 74 385 1.64e-86 269
MS.gene69420.t1 MTR_8g028460 40.785 331 175 7 104 420 164 487 4.02e-82 261
MS.gene69420.t1 MTR_0010s0400 41.667 324 172 9 104 421 147 459 6.35e-82 259
MS.gene69420.t1 MTR_4g094572 32.847 411 227 9 19 419 4 375 1.78e-62 206
MS.gene69420.t1 MTR_3g071100 100.000 41 0 0 204 244 23 63 1.49e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene69420.t1 AT3G27230 70.071 421 115 6 1 421 1 410 0.0 599
MS.gene69420.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene69420.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene69420.t1 AT5G40830 62.796 422 108 5 1 421 1 374 0.0 530
MS.gene69420.t1 AT3G05390 40.741 324 175 8 104 421 150 462 7.27e-84 264
MS.gene69420.t1 AT4G01240 41.667 348 173 10 89 420 323 656 9.22e-80 259
MS.gene69420.t1 AT1G29790 33.835 399 232 7 24 419 7 376 2.61e-71 229
MS.gene69420.t1 AT1G29790 33.835 399 232 7 24 419 7 376 2.61e-71 229

Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGTTTCCAAAGTGAATT+TGG 0.174013 3.3:-93046884 None:intergenic
CATTTCAGTGATTCCAGTTT+TGG 0.201099 3.3:-93046666 None:intergenic
CTGTTCTTCAGAAGCCTGTT+AGG 0.201673 3.3:+93047536 MS.gene69420:CDS
TTAGCAAAACACCTTCTTCT+AGG 0.243776 3.3:-93046824 None:intergenic
TGCTAAATCTGTTTCTTCTA+AGG 0.249692 3.3:+93046841 MS.gene69420:CDS
GGTTCTGTTTCTTTGAAAAT+TGG 0.270644 3.3:+93046302 MS.gene69420:CDS
TTTGATATTGGAATTGGTTC+TGG 0.272985 3.3:+93047109 MS.gene69420:CDS
TCAAAGACATTATCATAAAA+AGG 0.296087 3.3:-93047265 None:intergenic
TTAGTAACAAGACTGTTAAT+TGG 0.301382 3.3:+93046906 MS.gene69420:CDS
TTATGATAATGTCTTTGATT+TGG 0.302543 3.3:+93047270 MS.gene69420:CDS
TAACCCTCATAAACTTCCTT+TGG 0.314405 3.3:+93046634 MS.gene69420:CDS
TTGGGTAAAGATTCCAAAAC+TGG 0.315442 3.3:+93046653 MS.gene69420:CDS
TGCGGGCTGGTGGTTTGTTT+TGG 0.317509 3.3:+93047377 MS.gene69420:CDS
TTAACAGTCTTGTTACTAAC+AGG 0.318712 3.3:-93046902 None:intergenic
TCACTTGTTTACACCCTAAC+AGG 0.330154 3.3:-93047550 None:intergenic
TTGGATACAGAAAATTGAAA+TGG 0.350308 3.3:+93047464 MS.gene69420:CDS
ATAGGATTTGATATTGGAAT+TGG 0.350657 3.3:+93047103 MS.gene69420:CDS
ATTAGAATAGGATTTGATAT+TGG 0.361463 3.3:+93047097 MS.gene69420:CDS
TATATGAATTACAAAGTGTT+TGG 0.369620 3.3:+93046740 MS.gene69420:CDS
TTGCATGCATTGCTTTGATT+TGG 0.380204 3.3:+93046985 MS.gene69420:CDS
AACCCTCATAAACTTCCTTT+GGG 0.381617 3.3:+93046635 MS.gene69420:CDS
CAAAGTGTTTGGAAATTGTC+AGG 0.382386 3.3:+93046751 MS.gene69420:CDS
TTGGAAATTGTCAGGATGAT+TGG 0.389503 3.3:+93046759 MS.gene69420:CDS
ACAAGGTTGATTGAAAGGTT+TGG 0.397211 3.3:+93047445 MS.gene69420:CDS
GGAAATGGTGGAATTAGAAT+AGG 0.400305 3.3:+93047085 MS.gene69420:CDS
CATTGCTTTGATTTGGTGAA+TGG 0.407664 3.3:+93046992 MS.gene69420:CDS
CAAAACTGGAATCACTGAAA+TGG 0.411701 3.3:+93046667 MS.gene69420:CDS
TTACCCAAAGGAAGTTTATG+AGG 0.415254 3.3:-93046638 None:intergenic
TGGATACAGAAAATTGAAAT+GGG 0.423072 3.3:+93047465 MS.gene69420:CDS
CAGAAAATTGAAATGGGTTG+TGG 0.423087 3.3:+93047471 MS.gene69420:CDS
TTCCAAAGTGAATTTGGAAA+AGG 0.423351 3.3:-93046878 None:intergenic
CATTGACAAGGTTGATTGAA+AGG 0.426565 3.3:+93047440 MS.gene69420:CDS
CTTGGTGAAGTTGTTCGGTT+TGG 0.435606 3.3:-93046451 None:intergenic
TGGTAAGAAACAAGAGAAGT+TGG 0.449209 3.3:+93047324 MS.gene69420:CDS
TGTTCTTCAGAAGCCTGTTA+GGG 0.451195 3.3:+93047537 MS.gene69420:CDS
AGAAAATTGAAATGGGTTGT+GGG 0.464176 3.3:+93047472 MS.gene69420:CDS
AATGGTAAGAAATTGAATAG+AGG 0.470921 3.3:+93046962 MS.gene69420:CDS
GTGTACTTGGTGAAGTTGTT+CGG 0.472086 3.3:-93046456 None:intergenic
TGATCCAAGCTTAAGAAAAG+AGG 0.499670 3.3:-93047235 None:intergenic
CACTGAAATGGTTTCATCTG+TGG 0.507607 3.3:+93046679 MS.gene69420:CDS
TGAATGGTTATGAGAATCAA+AGG 0.511808 3.3:+93047008 MS.gene69420:CDS
GCAGCTATAAATTCATTGAA+AGG 0.519697 3.3:-93047199 None:intergenic
GGTTCGTGCTACAAATACCT+TGG 0.520674 3.3:+93047291 MS.gene69420:CDS
AACAAGACTGTTAATTGGAG+TGG 0.522922 3.3:+93046911 MS.gene69420:CDS
GTTTCTTTGAAAATTGGTGA+TGG 0.525785 3.3:+93046308 MS.gene69420:CDS
AAACACCTTCTTCTAGGTAA+AGG 0.526592 3.3:-93046818 None:intergenic
GAAATGGGTTGTGGGAGAAA+AGG 0.531934 3.3:+93047480 MS.gene69420:CDS
GTGGGAGAAAAGGTTGATAG+TGG 0.534948 3.3:+93047490 MS.gene69420:CDS
AATGAATTTATAGCTGCAAG+AGG 0.548634 3.3:+93047205 MS.gene69420:CDS
ACTGAAATGGTTTCATCTGT+GGG 0.553481 3.3:+93046680 MS.gene69420:CDS
TACCCAAAGGAAGTTTATGA+GGG 0.566733 3.3:-93046637 None:intergenic
GTGAGCCTTTACCTAGAAGA+AGG 0.567293 3.3:+93046813 MS.gene69420:CDS
TTGCTGCTGTAATGGCTGAA+AGG 0.580054 3.3:+93047137 MS.gene69420:CDS
AGAAGAAAATTTCATTGACA+AGG 0.582193 3.3:+93047428 MS.gene69420:CDS
CTTACCAACATCATCATCCA+AGG 0.591615 3.3:-93047308 None:intergenic
AATACCTTGGATGATGATGT+TGG 0.652086 3.3:+93047304 MS.gene69420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAATAGGATTTGATAT+TGG + chr3.3:93047097-93047116 MS.gene69420:CDS 20.0%
!! TCAAAGACATTATCATAAAA+AGG - chr3.3:93047268-93047287 None:intergenic 20.0%
!!! TATATGAATTACAAAGTGTT+TGG + chr3.3:93046740-93046759 MS.gene69420:CDS 20.0%
!!! TTATGATAATGTCTTTGATT+TGG + chr3.3:93047270-93047289 MS.gene69420:CDS 20.0%
! AATGGTAAGAAATTGAATAG+AGG + chr3.3:93046962-93046981 MS.gene69420:CDS 25.0%
! AGAAGAAAATTTCATTGACA+AGG + chr3.3:93047428-93047447 MS.gene69420:CDS 25.0%
! ATAGGATTTGATATTGGAAT+TGG + chr3.3:93047103-93047122 MS.gene69420:CDS 25.0%
! TGGATACAGAAAATTGAAAT+GGG + chr3.3:93047465-93047484 MS.gene69420:CDS 25.0%
! TTAGTAACAAGACTGTTAAT+TGG + chr3.3:93046906-93046925 MS.gene69420:CDS 25.0%
! TTGGATACAGAAAATTGAAA+TGG + chr3.3:93047464-93047483 MS.gene69420:CDS 25.0%
!!! AAAAAGATTGGTAGTGATAA+CGG - chr3.3:93046396-93046415 None:intergenic 25.0%
!!! AAGAATTTTGAGTGTTTGAA+TGG + chr3.3:93046944-93046963 MS.gene69420:CDS 25.0%
!!! CTCAGAAATTGATTTTGAAA+GGG + chr3.3:93046789-93046808 MS.gene69420:CDS 25.0%
AATGAATTTATAGCTGCAAG+AGG + chr3.3:93047205-93047224 MS.gene69420:CDS 30.0%
AGCATAAAGAGCAAAAAGAT+TGG - chr3.3:93046408-93046427 None:intergenic 30.0%
GCAGCTATAAATTCATTGAA+AGG - chr3.3:93047202-93047221 None:intergenic 30.0%
TGAATGGTTATGAGAATCAA+AGG + chr3.3:93047008-93047027 MS.gene69420:CDS 30.0%
TTCCAAAGTGAATTTGGAAA+AGG - chr3.3:93046881-93046900 None:intergenic 30.0%
! ATCCTTTTCCAAATTCACTT+TGG + chr3.3:93046876-93046895 MS.gene69420:CDS 30.0%
! GGTTCTGTTTCTTTGAAAAT+TGG + chr3.3:93046302-93046321 MS.gene69420:CDS 30.0%
! GTTTCTTTGAAAATTGGTGA+TGG + chr3.3:93046308-93046327 MS.gene69420:CDS 30.0%
! TGCTAAATCTGTTTCTTCTA+AGG + chr3.3:93046841-93046860 MS.gene69420:CDS 30.0%
! TTAAGCTCTTTTGCAATGTT+GGG - chr3.3:93046605-93046624 None:intergenic 30.0%
! TTTAAGCTCTTTTGCAATGT+TGG - chr3.3:93046606-93046625 None:intergenic 30.0%
! TTTGATATTGGAATTGGTTC+TGG + chr3.3:93047109-93047128 MS.gene69420:CDS 30.0%
! TTTTCCTCTTTTCTTAAGCT+TGG + chr3.3:93047231-93047250 MS.gene69420:CDS 30.0%
! TTTTCTTAAGCTTGGATCAT+AGG + chr3.3:93047239-93047258 MS.gene69420:CDS 30.0%
!! AGAAAATTGAAATGGGTTGT+GGG + chr3.3:93047472-93047491 MS.gene69420:CDS 30.0%
!! GCTCAGAAATTGATTTTGAA+AGG + chr3.3:93046788-93046807 MS.gene69420:CDS 30.0%
!! TTAACAGTCTTGTTACTAAC+AGG - chr3.3:93046905-93046924 None:intergenic 30.0%
!! TTTTCTTGCTGATGATGTTT+TGG + chr3.3:93047057-93047076 MS.gene69420:CDS 30.0%
!!! AAGAAGCTGTTTTTCTATCT+GGG - chr3.3:93046555-93046574 None:intergenic 30.0%
AAACACCTTCTTCTAGGTAA+AGG - chr3.3:93046821-93046840 None:intergenic 35.0%
AACAAGACTGTTAATTGGAG+TGG + chr3.3:93046911-93046930 MS.gene69420:CDS 35.0%
AACCCTCATAAACTTCCTTT+GGG + chr3.3:93046635-93046654 MS.gene69420:CDS 35.0%
ACAGGTTTCCAAAGTGAATT+TGG - chr3.3:93046887-93046906 None:intergenic 35.0%
ACTGAAATGGTTTCATCTGT+GGG + chr3.3:93046680-93046699 MS.gene69420:CDS 35.0%
CAAAACTGGAATCACTGAAA+TGG + chr3.3:93046667-93046686 MS.gene69420:CDS 35.0%
CAGATAGAAAAACAGCTTCT+TGG + chr3.3:93046554-93046573 MS.gene69420:CDS 35.0%
TAACCCTCATAAACTTCCTT+TGG + chr3.3:93046634-93046653 MS.gene69420:CDS 35.0%
TACCCAAAGGAAGTTTATGA+GGG - chr3.3:93046640-93046659 None:intergenic 35.0%
TGATCCAAGCTTAAGAAAAG+AGG - chr3.3:93047238-93047257 None:intergenic 35.0%
TGGTAAGAAACAAGAGAAGT+TGG + chr3.3:93047324-93047343 MS.gene69420:CDS 35.0%
TTACCCAAAGGAAGTTTATG+AGG - chr3.3:93046641-93046660 None:intergenic 35.0%
TTAGCAAAACACCTTCTTCT+AGG - chr3.3:93046827-93046846 None:intergenic 35.0%
TTGCATGCATTGCTTTGATT+TGG + chr3.3:93046985-93047004 MS.gene69420:CDS 35.0%
TTGGAAATTGTCAGGATGAT+TGG + chr3.3:93046759-93046778 MS.gene69420:CDS 35.0%
TTGGGTAAAGATTCCAAAAC+TGG + chr3.3:93046653-93046672 MS.gene69420:CDS 35.0%
! AATACCTTGGATGATGATGT+TGG + chr3.3:93047304-93047323 MS.gene69420:CDS 35.0%
! ACAAGGTTGATTGAAAGGTT+TGG + chr3.3:93047445-93047464 MS.gene69420:CDS 35.0%
! CAAAGTGTTTGGAAATTGTC+AGG + chr3.3:93046751-93046770 MS.gene69420:CDS 35.0%
! CATTGACAAGGTTGATTGAA+AGG + chr3.3:93047440-93047459 MS.gene69420:CDS 35.0%
! CATTTCAGTGATTCCAGTTT+TGG - chr3.3:93046669-93046688 None:intergenic 35.0%
! GAGAGAAGTTTTTGTGTACT+TGG - chr3.3:93046472-93046491 None:intergenic 35.0%
! GGAAATGGTGGAATTAGAAT+AGG + chr3.3:93047085-93047104 MS.gene69420:CDS 35.0%
!! CAGAAAATTGAAATGGGTTG+TGG + chr3.3:93047471-93047490 MS.gene69420:CDS 35.0%
!! CATTGCTTTGATTTGGTGAA+TGG + chr3.3:93046992-93047011 MS.gene69420:CDS 35.0%
!!! CAAGAAGCTGTTTTTCTATC+TGG - chr3.3:93046556-93046575 None:intergenic 35.0%
!!! GATGTTTTGGCATTAGGAAA+TGG + chr3.3:93047070-93047089 MS.gene69420:CDS 35.0%
!!! GTTTTGGAATCTTTACCCAA+AGG - chr3.3:93046653-93046672 None:intergenic 35.0%
CACTGAAATGGTTTCATCTG+TGG + chr3.3:93046679-93046698 MS.gene69420:CDS 40.0%
CTTACCAACATCATCATCCA+AGG - chr3.3:93047311-93047330 None:intergenic 40.0%
TGTTCTTCAGAAGCCTGTTA+GGG + chr3.3:93047537-93047556 MS.gene69420:CDS 40.0%
! GTGTACTTGGTGAAGTTGTT+CGG - chr3.3:93046459-93046478 None:intergenic 40.0%
! TCGACATCGATCGAATTTTG+CGG + chr3.3:93047359-93047378 MS.gene69420:CDS 40.0%
! TGGATCTTTTGCTGCTGTAA+TGG + chr3.3:93047129-93047148 MS.gene69420:CDS 40.0%
!! GTTTTGGCATTAGGAAATGG+TGG + chr3.3:93047073-93047092 MS.gene69420:CDS 40.0%
!!! GCTGATGATGTTTTGGCATT+AGG + chr3.3:93047064-93047083 MS.gene69420:CDS 40.0%
!!! TGTTCGGTTTGGCTGTTTTT+TGG - chr3.3:93046443-93046462 None:intergenic 40.0%
CTGTTCTTCAGAAGCCTGTT+AGG + chr3.3:93047536-93047555 MS.gene69420:CDS 45.0%
CTTGGTGAAGTTGTTCGGTT+TGG - chr3.3:93046454-93046473 None:intergenic 45.0%
GGTTCGTGCTACAAATACCT+TGG + chr3.3:93047291-93047310 MS.gene69420:CDS 45.0%
GTGAGCCTTTACCTAGAAGA+AGG + chr3.3:93046813-93046832 MS.gene69420:CDS 45.0%
TTGCTGCTGTAATGGCTGAA+AGG + chr3.3:93047137-93047156 MS.gene69420:CDS 45.0%
! CGACATCGATCGAATTTTGC+GGG + chr3.3:93047360-93047379 MS.gene69420:CDS 45.0%
!! GAAATGGGTTGTGGGAGAAA+AGG + chr3.3:93047480-93047499 MS.gene69420:CDS 45.0%
!! GTGGGAGAAAAGGTTGATAG+TGG + chr3.3:93047490-93047509 MS.gene69420:CDS 45.0%
! ATCGATCGAATTTTGCGGGC+TGG + chr3.3:93047364-93047383 MS.gene69420:CDS 50.0%
!!! GATCGAATTTTGCGGGCTGG+TGG + chr3.3:93047367-93047386 MS.gene69420:CDS 55.0%
!!! TGCGGGCTGGTGGTTTGTTT+TGG + chr3.3:93047377-93047396 MS.gene69420:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 93046299 93047564 93046299 ID=MS.gene69420
chr3.3 mRNA 93046299 93047564 93046299 ID=MS.gene69420.t1;Parent=MS.gene69420
chr3.3 exon 93046299 93047564 93046299 ID=MS.gene69420.t1.exon1;Parent=MS.gene69420.t1
chr3.3 CDS 93046299 93047564 93046299 ID=cds.MS.gene69420.t1;Parent=MS.gene69420.t1
Gene Sequence

>MS.gene69420

ATGGGTTCTGTTTCTTTGAAAATTGGTGATGGAACAGCAAGATTCAAAAAATCAACACTTTGTTCTTCAGCTGTTAACATTCTCATGATCTTATCCGTTATCACTACCAATCTTTTTGCTCTTTATGCTTTCACTTCTTCTCCAAAAAACAGCCAAACCGAACAACTTCACCAAGTACACAAAAACTTCTCTCTCATCTCAGAACAAGTTTCACTTATACTCAGAGAAATTGATCAATCTCAAAAGAAACTAGCCCAGATAGAAAAACAGCTTCTTGGCTATGAAAGTTTTGATCTTTCAAGACCCAACATTGCAAAAGAGCTTAAACTCTTTCTTAACCCTCATAAACTTCCTTTGGGTAAAGATTCCAAAACTGGAATCACTGAAATGGTTTCATCTGTGGGTCATTCTTGTGAGAAATCTTCTGATTTGTTGTCTCAGTATATGAATTACAAAGTGTTTGGAAATTGTCAGGATGATTGGAGTTATGCTCAGAAATTGATTTTGAAAGGGTGTGAGCCTTTACCTAGAAGAAGGTGTTTTGCTAAATCTGTTTCTTCTAAGGTAAATTATCTTCATCCTTTTCCAAATTCACTTTGGAAACCTGTTAGTAACAAGACTGTTAATTGGAGTGGTTTTAGTTGTAAGAATTTTGAGTGTTTGAATGGTAAGAAATTGAATAGAGGTTGCATGCATTGCTTTGATTTGGTGAATGGTTATGAGAATCAAAGGTTTGTTAAGTCTAGAAGTAAGAATGATTTTCTTGCTGATGATGTTTTGGCATTAGGAAATGGTGGAATTAGAATAGGATTTGATATTGGAATTGGTTCTGGATCTTTTGCTGCTGTAATGGCTGAAAGGAATGTTACAATTGTTACTAGTACTCTTAATATTGATGCTCCTTTCAATGAATTTATAGCTGCAAGAGGACTTTTTCCTCTTTTCTTAAGCTTGGATCATAGGTTTCCTTTTTATGATAATGTCTTTGATTTGGTTCGTGCTACAAATACCTTGGATGATGATGTTGGTAAGAAACAAGAGAAGTTGGAGTTTTTAATGTTCGACATCGATCGAATTTTGCGGGCTGGTGGTTTGTTTTGGATTGATAACTTCTATTGTGCTAATGATGAGAAGAAAATTTCATTGACAAGGTTGATTGAAAGGTTTGGATACAGAAAATTGAAATGGGTTGTGGGAGAAAAGGTTGATAGTGGTAAGTCTCAAGTTTTCTTATCTGCTGTTCTTCAGAAGCCTGTTAGGGTGTAA

Protein sequence

>MS.gene69420.t1

MGSVSLKIGDGTARFKKSTLCSSAVNILMILSVITTNLFALYAFTSSPKNSQTEQLHQVHKNFSLISEQVSLILREIDQSQKKLAQIEKQLLGYESFDLSRPNIAKELKLFLNPHKLPLGKDSKTGITEMVSSVGHSCEKSSDLLSQYMNYKVFGNCQDDWSYAQKLILKGCEPLPRRRCFAKSVSSKVNYLHPFPNSLWKPVSNKTVNWSGFSCKNFECLNGKKLNRGCMHCFDLVNGYENQRFVKSRSKNDFLADDVLALGNGGIRIGFDIGIGSGSFAAVMAERNVTIVTSTLNIDAPFNEFIAARGLFPLFLSLDHRFPFYDNVFDLVRATNTLDDDVGKKQEKLEFLMFDIDRILRAGGLFWIDNFYCANDEKKISLTRLIERFGYRKLKWVVGEKVDSGKSQVFLSAVLQKPVRV