AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene064739


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064739.t1 MTR_3g113270 97.387 421 11 0 1 421 1 421 0.0 848
MS.gene064739.t1 MTR_1g024005 76.457 429 88 5 1 420 1 425 0.0 656
MS.gene064739.t1 MTR_3g071050 97.358 265 7 0 29 293 1 265 0.0 540
MS.gene064739.t1 MTR_3g070620 97.037 135 4 0 225 359 54 188 2.49e-91 274
MS.gene064739.t1 MTR_2g028590 40.053 377 208 8 56 419 92 463 5.20e-89 278
MS.gene064739.t1 MTR_2g028610 37.123 431 224 11 30 419 34 458 9.37e-89 277
MS.gene064739.t1 MTR_5g077710 42.722 316 169 8 113 420 74 385 1.64e-86 269
MS.gene064739.t1 MTR_8g028460 40.785 331 175 7 104 420 164 487 4.02e-82 261
MS.gene064739.t1 MTR_0010s0400 41.667 324 172 9 104 421 147 459 6.35e-82 259
MS.gene064739.t1 MTR_4g094572 32.847 411 227 9 19 419 4 375 1.78e-62 206
MS.gene064739.t1 MTR_3g071100 100.000 41 0 0 204 244 23 63 1.49e-23 93.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene064739.t1 AT3G27230 70.071 421 115 6 1 421 1 410 0.0 599
MS.gene064739.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene064739.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene064739.t1 AT5G40830 62.796 422 108 5 1 421 1 374 0.0 530
MS.gene064739.t1 AT3G05390 40.741 324 175 8 104 421 150 462 7.27e-84 264
MS.gene064739.t1 AT4G01240 41.667 348 173 10 89 420 323 656 9.22e-80 259
MS.gene064739.t1 AT1G29790 33.835 399 232 7 24 419 7 376 2.61e-71 229
MS.gene064739.t1 AT1G29790 33.835 399 232 7 24 419 7 376 2.61e-71 229

Find 56 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGTTTCCAAAGTGAATT+TGG 0.174013 3.4:-97694953 None:intergenic
CATTTCAGTGATTCCAGTTT+TGG 0.201099 3.4:-97694735 None:intergenic
CTGTTCTTCAGAAGCCTGTT+AGG 0.201673 3.4:+97695605 MS.gene064739:CDS
TTAGCAAAACACCTTCTTCT+AGG 0.243776 3.4:-97694893 None:intergenic
TGCTAAATCTGTTTCTTCTA+AGG 0.249692 3.4:+97694910 MS.gene064739:CDS
GGTTCTGTTTCTTTGAAAAT+TGG 0.270644 3.4:+97694371 MS.gene064739:CDS
TTTGATATTGGAATTGGTTC+TGG 0.272985 3.4:+97695178 MS.gene064739:CDS
TCAAAGACATTATCATAAAA+AGG 0.296087 3.4:-97695334 None:intergenic
TTAGTAACAAGACTGTTAAT+TGG 0.301382 3.4:+97694975 MS.gene064739:CDS
TTATGATAATGTCTTTGATT+TGG 0.302543 3.4:+97695339 MS.gene064739:CDS
TAACCCTCATAAACTTCCTT+TGG 0.314405 3.4:+97694703 MS.gene064739:CDS
TTGGGTAAAGATTCCAAAAC+TGG 0.315442 3.4:+97694722 MS.gene064739:CDS
TGCGGGCTGGTGGTTTGTTT+TGG 0.317509 3.4:+97695446 MS.gene064739:CDS
TTAACAGTCTTGTTACTAAC+AGG 0.318712 3.4:-97694971 None:intergenic
TCACTTGTTTACACCCTAAC+AGG 0.330154 3.4:-97695619 None:intergenic
TTGGATACAGAAAATTGAAA+TGG 0.350308 3.4:+97695533 MS.gene064739:CDS
ATAGGATTTGATATTGGAAT+TGG 0.350657 3.4:+97695172 MS.gene064739:CDS
ATTAGAATAGGATTTGATAT+TGG 0.361463 3.4:+97695166 MS.gene064739:CDS
TATATGAATTACAAAGTGTT+TGG 0.369620 3.4:+97694809 MS.gene064739:CDS
TTGCATGCATTGCTTTGATT+TGG 0.380204 3.4:+97695054 MS.gene064739:CDS
AACCCTCATAAACTTCCTTT+GGG 0.381617 3.4:+97694704 MS.gene064739:CDS
CAAAGTGTTTGGAAATTGTC+AGG 0.382386 3.4:+97694820 MS.gene064739:CDS
TTGGAAATTGTCAGGATGAT+TGG 0.389503 3.4:+97694828 MS.gene064739:CDS
ACAAGGTTGATTGAAAGGTT+TGG 0.397211 3.4:+97695514 MS.gene064739:CDS
GGAAATGGTGGAATTAGAAT+AGG 0.400305 3.4:+97695154 MS.gene064739:CDS
CATTGCTTTGATTTGGTGAA+TGG 0.407664 3.4:+97695061 MS.gene064739:CDS
CAAAACTGGAATCACTGAAA+TGG 0.411701 3.4:+97694736 MS.gene064739:CDS
TTACCCAAAGGAAGTTTATG+AGG 0.415254 3.4:-97694707 None:intergenic
TGGATACAGAAAATTGAAAT+GGG 0.423072 3.4:+97695534 MS.gene064739:CDS
CAGAAAATTGAAATGGGTTG+TGG 0.423087 3.4:+97695540 MS.gene064739:CDS
TTCCAAAGTGAATTTGGAAA+AGG 0.423351 3.4:-97694947 None:intergenic
CATTGACAAGGTTGATTGAA+AGG 0.426565 3.4:+97695509 MS.gene064739:CDS
CTTGGTGAAGTTGTTCGGTT+TGG 0.435606 3.4:-97694520 None:intergenic
TGGTAAGAAACAAGAGAAGT+TGG 0.449209 3.4:+97695393 MS.gene064739:CDS
TGTTCTTCAGAAGCCTGTTA+GGG 0.451195 3.4:+97695606 MS.gene064739:CDS
AGAAAATTGAAATGGGTTGT+GGG 0.464176 3.4:+97695541 MS.gene064739:CDS
AATGGTAAGAAATTGAATAG+AGG 0.470921 3.4:+97695031 MS.gene064739:CDS
GTGTACTTGGTGAAGTTGTT+CGG 0.472086 3.4:-97694525 None:intergenic
TGATCCAAGCTTAAGAAAAG+AGG 0.499670 3.4:-97695304 None:intergenic
CACTGAAATGGTTTCATCTG+TGG 0.507607 3.4:+97694748 MS.gene064739:CDS
TGAATGGTTATGAGAATCAA+AGG 0.511808 3.4:+97695077 MS.gene064739:CDS
GCAGCTATAAATTCATTGAA+AGG 0.519697 3.4:-97695268 None:intergenic
GGTTCGTGCTACAAATACCT+TGG 0.520674 3.4:+97695360 MS.gene064739:CDS
AACAAGACTGTTAATTGGAG+TGG 0.522922 3.4:+97694980 MS.gene064739:CDS
GTTTCTTTGAAAATTGGTGA+TGG 0.525785 3.4:+97694377 MS.gene064739:CDS
AAACACCTTCTTCTAGGTAA+AGG 0.526592 3.4:-97694887 None:intergenic
GAAATGGGTTGTGGGAGAAA+AGG 0.531934 3.4:+97695549 MS.gene064739:CDS
GTGGGAGAAAAGGTTGATAG+TGG 0.534948 3.4:+97695559 MS.gene064739:CDS
AATGAATTTATAGCTGCAAG+AGG 0.548634 3.4:+97695274 MS.gene064739:CDS
ACTGAAATGGTTTCATCTGT+GGG 0.553481 3.4:+97694749 MS.gene064739:CDS
TACCCAAAGGAAGTTTATGA+GGG 0.566733 3.4:-97694706 None:intergenic
GTGAGCCTTTACCTAGAAGA+AGG 0.567293 3.4:+97694882 MS.gene064739:CDS
TTGCTGCTGTAATGGCTGAA+AGG 0.580054 3.4:+97695206 MS.gene064739:CDS
AGAAGAAAATTTCATTGACA+AGG 0.582193 3.4:+97695497 MS.gene064739:CDS
CTTACCAACATCATCATCCA+AGG 0.591615 3.4:-97695377 None:intergenic
AATACCTTGGATGATGATGT+TGG 0.652086 3.4:+97695373 MS.gene064739:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGAATAGGATTTGATAT+TGG + chr3.4:97695166-97695185 MS.gene064739:CDS 20.0%
!! TCAAAGACATTATCATAAAA+AGG - chr3.4:97695337-97695356 None:intergenic 20.0%
!!! TATATGAATTACAAAGTGTT+TGG + chr3.4:97694809-97694828 MS.gene064739:CDS 20.0%
!!! TTATGATAATGTCTTTGATT+TGG + chr3.4:97695339-97695358 MS.gene064739:CDS 20.0%
! AATGGTAAGAAATTGAATAG+AGG + chr3.4:97695031-97695050 MS.gene064739:CDS 25.0%
! AGAAGAAAATTTCATTGACA+AGG + chr3.4:97695497-97695516 MS.gene064739:CDS 25.0%
! ATAGGATTTGATATTGGAAT+TGG + chr3.4:97695172-97695191 MS.gene064739:CDS 25.0%
! TGGATACAGAAAATTGAAAT+GGG + chr3.4:97695534-97695553 MS.gene064739:CDS 25.0%
! TTAGTAACAAGACTGTTAAT+TGG + chr3.4:97694975-97694994 MS.gene064739:CDS 25.0%
! TTGGATACAGAAAATTGAAA+TGG + chr3.4:97695533-97695552 MS.gene064739:CDS 25.0%
!! AAAAAGATTGGTTGTGATAA+CGG - chr3.4:97694465-97694484 None:intergenic 25.0%
!!! AAGAATTTTGAGTGTTTGAA+TGG + chr3.4:97695013-97695032 MS.gene064739:CDS 25.0%
!!! CTCAGAAATTGATTTTGAAA+GGG + chr3.4:97694858-97694877 MS.gene064739:CDS 25.0%
AATGAATTTATAGCTGCAAG+AGG + chr3.4:97695274-97695293 MS.gene064739:CDS 30.0%
AGCATAAAGAGCAAAAAGAT+TGG - chr3.4:97694477-97694496 None:intergenic 30.0%
GCAGCTATAAATTCATTGAA+AGG - chr3.4:97695271-97695290 None:intergenic 30.0%
TGAATGGTTATGAGAATCAA+AGG + chr3.4:97695077-97695096 MS.gene064739:CDS 30.0%
TTCCAAAGTGAATTTGGAAA+AGG - chr3.4:97694950-97694969 None:intergenic 30.0%
! ATCCTTTTCCAAATTCACTT+TGG + chr3.4:97694945-97694964 MS.gene064739:CDS 30.0%
! GGTTCTGTTTCTTTGAAAAT+TGG + chr3.4:97694371-97694390 MS.gene064739:CDS 30.0%
! GTTTCTTTGAAAATTGGTGA+TGG + chr3.4:97694377-97694396 MS.gene064739:CDS 30.0%
! TGCTAAATCTGTTTCTTCTA+AGG + chr3.4:97694910-97694929 MS.gene064739:CDS 30.0%
! TTAAGCTCTTTTGCAATGTT+GGG - chr3.4:97694674-97694693 None:intergenic 30.0%
! TTTAAGCTCTTTTGCAATGT+TGG - chr3.4:97694675-97694694 None:intergenic 30.0%
! TTTGATATTGGAATTGGTTC+TGG + chr3.4:97695178-97695197 MS.gene064739:CDS 30.0%
! TTTTCCTCTTTTCTTAAGCT+TGG + chr3.4:97695300-97695319 MS.gene064739:CDS 30.0%
! TTTTCTTAAGCTTGGATCAT+AGG + chr3.4:97695308-97695327 MS.gene064739:CDS 30.0%
!! AGAAAATTGAAATGGGTTGT+GGG + chr3.4:97695541-97695560 MS.gene064739:CDS 30.0%
!! GCTCAGAAATTGATTTTGAA+AGG + chr3.4:97694857-97694876 MS.gene064739:CDS 30.0%
!! TTAACAGTCTTGTTACTAAC+AGG - chr3.4:97694974-97694993 None:intergenic 30.0%
!! TTTTCTTGCTGATGATGTTT+TGG + chr3.4:97695126-97695145 MS.gene064739:CDS 30.0%
!!! AAGAAGCTGTTTTTCTATCT+GGG - chr3.4:97694624-97694643 None:intergenic 30.0%
AAACACCTTCTTCTAGGTAA+AGG - chr3.4:97694890-97694909 None:intergenic 35.0%
AACAAGACTGTTAATTGGAG+TGG + chr3.4:97694980-97694999 MS.gene064739:CDS 35.0%
AACCCTCATAAACTTCCTTT+GGG + chr3.4:97694704-97694723 MS.gene064739:CDS 35.0%
ACAGGTTTCCAAAGTGAATT+TGG - chr3.4:97694956-97694975 None:intergenic 35.0%
ACTGAAATGGTTTCATCTGT+GGG + chr3.4:97694749-97694768 MS.gene064739:CDS 35.0%
CAAAACTGGAATCACTGAAA+TGG + chr3.4:97694736-97694755 MS.gene064739:CDS 35.0%
CAGATAGAAAAACAGCTTCT+TGG + chr3.4:97694623-97694642 MS.gene064739:CDS 35.0%
TAACCCTCATAAACTTCCTT+TGG + chr3.4:97694703-97694722 MS.gene064739:CDS 35.0%
TACCCAAAGGAAGTTTATGA+GGG - chr3.4:97694709-97694728 None:intergenic 35.0%
TGATCCAAGCTTAAGAAAAG+AGG - chr3.4:97695307-97695326 None:intergenic 35.0%
TGGTAAGAAACAAGAGAAGT+TGG + chr3.4:97695393-97695412 MS.gene064739:CDS 35.0%
TTACCCAAAGGAAGTTTATG+AGG - chr3.4:97694710-97694729 None:intergenic 35.0%
TTAGCAAAACACCTTCTTCT+AGG - chr3.4:97694896-97694915 None:intergenic 35.0%
TTGCATGCATTGCTTTGATT+TGG + chr3.4:97695054-97695073 MS.gene064739:CDS 35.0%
TTGGAAATTGTCAGGATGAT+TGG + chr3.4:97694828-97694847 MS.gene064739:CDS 35.0%
TTGGGTAAAGATTCCAAAAC+TGG + chr3.4:97694722-97694741 MS.gene064739:CDS 35.0%
! AATACCTTGGATGATGATGT+TGG + chr3.4:97695373-97695392 MS.gene064739:CDS 35.0%
! ACAAGGTTGATTGAAAGGTT+TGG + chr3.4:97695514-97695533 MS.gene064739:CDS 35.0%
! CAAAGTGTTTGGAAATTGTC+AGG + chr3.4:97694820-97694839 MS.gene064739:CDS 35.0%
! CATTGACAAGGTTGATTGAA+AGG + chr3.4:97695509-97695528 MS.gene064739:CDS 35.0%
! CATTTCAGTGATTCCAGTTT+TGG - chr3.4:97694738-97694757 None:intergenic 35.0%
! GAGAGAAGTTTTTGTGTACT+TGG - chr3.4:97694541-97694560 None:intergenic 35.0%
! GGAAATGGTGGAATTAGAAT+AGG + chr3.4:97695154-97695173 MS.gene064739:CDS 35.0%
!! CAGAAAATTGAAATGGGTTG+TGG + chr3.4:97695540-97695559 MS.gene064739:CDS 35.0%
!! CATTGCTTTGATTTGGTGAA+TGG + chr3.4:97695061-97695080 MS.gene064739:CDS 35.0%
!!! CAAGAAGCTGTTTTTCTATC+TGG - chr3.4:97694625-97694644 None:intergenic 35.0%
!!! GATGTTTTGGCATTAGGAAA+TGG + chr3.4:97695139-97695158 MS.gene064739:CDS 35.0%
!!! GTTTTGGAATCTTTACCCAA+AGG - chr3.4:97694722-97694741 None:intergenic 35.0%
CACTGAAATGGTTTCATCTG+TGG + chr3.4:97694748-97694767 MS.gene064739:CDS 40.0%
CTTACCAACATCATCATCCA+AGG - chr3.4:97695380-97695399 None:intergenic 40.0%
TGTTCTTCAGAAGCCTGTTA+GGG + chr3.4:97695606-97695625 MS.gene064739:CDS 40.0%
! GTGTACTTGGTGAAGTTGTT+CGG - chr3.4:97694528-97694547 None:intergenic 40.0%
! TCGACATCGATCGAATTTTG+CGG + chr3.4:97695428-97695447 MS.gene064739:CDS 40.0%
! TGGATCTTTTGCTGCTGTAA+TGG + chr3.4:97695198-97695217 MS.gene064739:CDS 40.0%
!! GTTTTGGCATTAGGAAATGG+TGG + chr3.4:97695142-97695161 MS.gene064739:CDS 40.0%
!!! GCTGATGATGTTTTGGCATT+AGG + chr3.4:97695133-97695152 MS.gene064739:CDS 40.0%
!!! TGTTCGGTTTGGCTGTTTTT+TGG - chr3.4:97694512-97694531 None:intergenic 40.0%
CTGTTCTTCAGAAGCCTGTT+AGG + chr3.4:97695605-97695624 MS.gene064739:CDS 45.0%
CTTGGTGAAGTTGTTCGGTT+TGG - chr3.4:97694523-97694542 None:intergenic 45.0%
GGTTCGTGCTACAAATACCT+TGG + chr3.4:97695360-97695379 MS.gene064739:CDS 45.0%
GTGAGCCTTTACCTAGAAGA+AGG + chr3.4:97694882-97694901 MS.gene064739:CDS 45.0%
TTGCTGCTGTAATGGCTGAA+AGG + chr3.4:97695206-97695225 MS.gene064739:CDS 45.0%
! CGACATCGATCGAATTTTGC+GGG + chr3.4:97695429-97695448 MS.gene064739:CDS 45.0%
!! GAAATGGGTTGTGGGAGAAA+AGG + chr3.4:97695549-97695568 MS.gene064739:CDS 45.0%
!! GTGGGAGAAAAGGTTGATAG+TGG + chr3.4:97695559-97695578 MS.gene064739:CDS 45.0%
! ATCGATCGAATTTTGCGGGC+TGG + chr3.4:97695433-97695452 MS.gene064739:CDS 50.0%
!!! GATCGAATTTTGCGGGCTGG+TGG + chr3.4:97695436-97695455 MS.gene064739:CDS 55.0%
!!! TGCGGGCTGGTGGTTTGTTT+TGG + chr3.4:97695446-97695465 MS.gene064739:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 97694368 97695633 97694368 ID=MS.gene064739
chr3.4 mRNA 97694368 97695633 97694368 ID=MS.gene064739.t1;Parent=MS.gene064739
chr3.4 exon 97694368 97695633 97694368 ID=MS.gene064739.t1.exon1;Parent=MS.gene064739.t1
chr3.4 CDS 97694368 97695633 97694368 ID=cds.MS.gene064739.t1;Parent=MS.gene064739.t1
Gene Sequence

>MS.gene064739

ATGGGTTCTGTTTCTTTGAAAATTGGTGATGGAACAGCAAGATTCAAAAAATCAACACTTTGTTCTTCAGCTGTTAACATTCTCATGATCTTATCCGTTATCACAACCAATCTTTTTGCTCTTTATGCTTTCACTTCTTCTCCAAAAAACAGCCAAACCGAACAACTTCACCAAGTACACAAAAACTTCTCTCTCATCTCAGAACAAGTTTCACTTATACTCAGAGAAATTGATCAATCTCAAAAGAAACTAGCCCAGATAGAAAAACAGCTTCTTGGCTATGAAAGTTTTGATCTTTCAAGACCCAACATTGCAAAAGAGCTTAAACTCTTTCTTAACCCTCATAAACTTCCTTTGGGTAAAGATTCCAAAACTGGAATCACTGAAATGGTTTCATCTGTGGGTCATTCTTGTGAGAAATCTTCTGATTTGTTGTCTCAGTATATGAATTACAAAGTGTTTGGAAATTGTCAGGATGATTGGAGTTATGCTCAGAAATTGATTTTGAAAGGGTGTGAGCCTTTACCTAGAAGAAGGTGTTTTGCTAAATCTGTTTCTTCTAAGGTAAATTATCTTCATCCTTTTCCAAATTCACTTTGGAAACCTGTTAGTAACAAGACTGTTAATTGGAGTGGTTTTAGTTGTAAGAATTTTGAGTGTTTGAATGGTAAGAAATTGAATAGAGGTTGCATGCATTGCTTTGATTTGGTGAATGGTTATGAGAATCAAAGGTTTGTTAAGTCTAGAAGTAAGAATGATTTTCTTGCTGATGATGTTTTGGCATTAGGAAATGGTGGAATTAGAATAGGATTTGATATTGGAATTGGTTCTGGATCTTTTGCTGCTGTAATGGCTGAAAGGAATGTTACAATTGTTACTAGTACTCTTAATATTGATGCTCCTTTCAATGAATTTATAGCTGCAAGAGGACTTTTTCCTCTTTTCTTAAGCTTGGATCATAGGTTTCCTTTTTATGATAATGTCTTTGATTTGGTTCGTGCTACAAATACCTTGGATGATGATGTTGGTAAGAAACAAGAGAAGTTGGAGTTTTTAATGTTCGACATCGATCGAATTTTGCGGGCTGGTGGTTTGTTTTGGATTGATAACTTCTATTGTGCTAATGATGAGAAGAAAATTTCATTGACAAGGTTGATTGAAAGGTTTGGATACAGAAAATTGAAATGGGTTGTGGGAGAAAAGGTTGATAGTGGTAAGTCTCAAGTTTTCTTATCTGCTGTTCTTCAGAAGCCTGTTAGGGTGTAA

Protein sequence

>MS.gene064739.t1

MGSVSLKIGDGTARFKKSTLCSSAVNILMILSVITTNLFALYAFTSSPKNSQTEQLHQVHKNFSLISEQVSLILREIDQSQKKLAQIEKQLLGYESFDLSRPNIAKELKLFLNPHKLPLGKDSKTGITEMVSSVGHSCEKSSDLLSQYMNYKVFGNCQDDWSYAQKLILKGCEPLPRRRCFAKSVSSKVNYLHPFPNSLWKPVSNKTVNWSGFSCKNFECLNGKKLNRGCMHCFDLVNGYENQRFVKSRSKNDFLADDVLALGNGGIRIGFDIGIGSGSFAAVMAERNVTIVTSTLNIDAPFNEFIAARGLFPLFLSLDHRFPFYDNVFDLVRATNTLDDDVGKKQEKLEFLMFDIDRILRAGGLFWIDNFYCANDEKKISLTRLIERFGYRKLKWVVGEKVDSGKSQVFLSAVLQKPVRV