AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42277


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42277.t1 MTR_3g113270 96.912 421 13 0 1 421 1 421 0.0 843
MS.gene42277.t1 MTR_1g024005 76.457 429 88 5 1 420 1 425 0.0 654
MS.gene42277.t1 MTR_3g071050 96.604 265 9 0 29 293 1 265 0.0 535
MS.gene42277.t1 MTR_3g070620 97.037 135 4 0 225 359 54 188 2.80e-91 274
MS.gene42277.t1 MTR_2g028590 40.053 377 208 8 56 419 92 463 1.38e-88 276
MS.gene42277.t1 MTR_2g028610 37.123 431 224 11 30 419 34 458 2.75e-88 276
MS.gene42277.t1 MTR_5g077710 42.405 316 170 8 113 420 74 385 6.39e-86 267
MS.gene42277.t1 MTR_8g028460 40.785 331 175 7 104 420 164 487 8.51e-82 260
MS.gene42277.t1 MTR_0010s0400 41.667 324 172 9 104 421 147 459 1.50e-81 258
MS.gene42277.t1 MTR_4g094572 32.847 411 227 9 19 419 4 375 2.70e-62 206
MS.gene42277.t1 MTR_3g071100 97.500 40 1 0 205 244 24 63 8.16e-22 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42277.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene42277.t1 AT5G40830 68.483 422 124 4 1 421 1 414 0.0 595
MS.gene42277.t1 AT3G27230 69.596 421 117 6 1 421 1 410 0.0 594
MS.gene42277.t1 AT5G40830 63.033 422 107 5 1 421 1 374 0.0 532
MS.gene42277.t1 AT3G05390 40.741 324 175 8 104 421 150 462 1.89e-83 263
MS.gene42277.t1 AT4G01240 41.667 348 173 10 89 420 323 656 2.17e-79 258
MS.gene42277.t1 AT1G29790 33.584 399 233 7 24 419 7 376 5.47e-71 228
MS.gene42277.t1 AT1G29790 33.584 399 233 7 24 419 7 376 5.47e-71 228

Find 57 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGTTTCCAAAGTGAATT+TGG 0.170193 3.2:-90075875 None:intergenic
CATTTCAGTGATTCCAGTTT+TGG 0.201099 3.2:-90075657 None:intergenic
CTGTTCTTCAGAAGCCTGTT+AGG 0.201673 3.2:+90076527 MS.gene42277:CDS
TTAGCAAAACACCTTCTTCT+AGG 0.243776 3.2:-90075815 None:intergenic
TGCTAAATCTGTTTCTTCTA+AGG 0.249692 3.2:+90075832 MS.gene42277:CDS
GGTTCTGTTTCTTTGAAAAT+TGG 0.270644 3.2:+90075293 MS.gene42277:CDS
TTTGATATTGGAATTGGTTC+TGG 0.272985 3.2:+90076100 MS.gene42277:CDS
TCAAAGACATTATCATAAAA+AGG 0.296087 3.2:-90076256 None:intergenic
TTATGATAATGTCTTTGATT+TGG 0.302543 3.2:+90076261 MS.gene42277:CDS
TAACCCTCATAAACTTCCTT+TGG 0.314405 3.2:+90075625 MS.gene42277:CDS
TTGGGTAAAGATTCCAAAAC+TGG 0.315442 3.2:+90075644 MS.gene42277:CDS
TGCGGGCTGGTGGTTTGTTT+TGG 0.317509 3.2:+90076368 MS.gene42277:CDS
TCACTTGTTTACACCCTAAC+AGG 0.330154 3.2:-90076541 None:intergenic
TTGGATACAGAAAATTGAAA+TGG 0.350308 3.2:+90076455 MS.gene42277:CDS
ATAGGATTTGATATTGGAAT+TGG 0.350657 3.2:+90076094 MS.gene42277:CDS
ATTAGAATAGGATTTGATAT+TGG 0.361463 3.2:+90076088 MS.gene42277:CDS
TATATGAATTACAAAGTGTT+TGG 0.369620 3.2:+90075731 MS.gene42277:CDS
TTGGTAACAAGACTGTTAAT+TGG 0.379100 3.2:+90075897 MS.gene42277:CDS
TTGCATGCATTGCTTTGATT+TGG 0.380204 3.2:+90075976 MS.gene42277:CDS
AACCCTCATAAACTTCCTTT+GGG 0.381617 3.2:+90075626 MS.gene42277:CDS
CAAAGTGTTTGGAAATTGTC+AGG 0.382386 3.2:+90075742 MS.gene42277:CDS
TTGGAAATTGTCAGGATGAT+TGG 0.389503 3.2:+90075750 MS.gene42277:CDS
ACAAGGTTGATTGAAAGGTT+TGG 0.397211 3.2:+90076436 MS.gene42277:CDS
GGAAATGGTGGAATTAGAAT+AGG 0.400305 3.2:+90076076 MS.gene42277:CDS
CATTGCTTTGATTTGGTGAA+TGG 0.407664 3.2:+90075983 MS.gene42277:CDS
CAAAACTGGAATCACTGAAA+TGG 0.411701 3.2:+90075658 MS.gene42277:CDS
TTACCCAAAGGAAGTTTATG+AGG 0.415254 3.2:-90075629 None:intergenic
TGGATACAGAAAATTGAAAT+GGG 0.423072 3.2:+90076456 MS.gene42277:CDS
CAGAAAATTGAAATGGGTTG+TGG 0.423087 3.2:+90076462 MS.gene42277:CDS
TTCCAAAGTGAATTTGGAAA+AGG 0.423351 3.2:-90075869 None:intergenic
CATTGACAAGGTTGATTGAA+AGG 0.426565 3.2:+90076431 MS.gene42277:CDS
CTTGGTGAAGTTGTTCGGTT+TGG 0.435606 3.2:-90075442 None:intergenic
TTAACAGTCTTGTTACCAAC+AGG 0.440808 3.2:-90075893 None:intergenic
TGGTAAGAAACAAGAGAAGT+TGG 0.449209 3.2:+90076315 MS.gene42277:CDS
TGTTCTTCAGAAGCCTGTTA+GGG 0.451195 3.2:+90076528 MS.gene42277:CDS
AGAAAATTGAAATGGGTTGT+GGG 0.464176 3.2:+90076463 MS.gene42277:CDS
GTGTACTTGGTGAAGTTGTT+CGG 0.472086 3.2:-90075447 None:intergenic
TGATCCAAGCTTAAGAAAAG+AGG 0.499670 3.2:-90076226 None:intergenic
CACTGAAATGGTTTCATCTG+TGG 0.507607 3.2:+90075670 MS.gene42277:CDS
TGAATGGTTATGAGAATCAA+AGG 0.511808 3.2:+90075999 MS.gene42277:CDS
GCAGCTATAAATTCATTGAA+AGG 0.519697 3.2:-90076190 None:intergenic
GGTTCGTGCTACAAATACCT+TGG 0.520674 3.2:+90076282 MS.gene42277:CDS
AACAAGACTGTTAATTGGAG+TGG 0.522922 3.2:+90075902 MS.gene42277:CDS
AATTCACTTTGGAAACCTGT+TGG 0.524197 3.2:+90075878 MS.gene42277:CDS
GTTTCTTTGAAAATTGGTGA+TGG 0.525785 3.2:+90075299 MS.gene42277:CDS
AAACACCTTCTTCTAGGTAA+AGG 0.526592 3.2:-90075809 None:intergenic
GAAATGGGTTGTGGGAGAAA+AGG 0.531934 3.2:+90076471 MS.gene42277:CDS
GTGGGAGAAAAGGTTGATAG+TGG 0.534948 3.2:+90076481 MS.gene42277:CDS
AATGTTAAGAAATTGAATAG+AGG 0.538968 3.2:+90075953 MS.gene42277:CDS
AATGAATTTATAGCTGCAAG+AGG 0.548634 3.2:+90076196 MS.gene42277:CDS
ACTGAAATGGTTTCATCTGT+GGG 0.553481 3.2:+90075671 MS.gene42277:CDS
TACCCAAAGGAAGTTTATGA+GGG 0.566733 3.2:-90075628 None:intergenic
GTGAGCCTTTACCTAGAAGA+AGG 0.567293 3.2:+90075804 MS.gene42277:CDS
TTGCTGCTGTAATGGCTGAA+AGG 0.580054 3.2:+90076128 MS.gene42277:CDS
AGAAGAAAATTTCATTGACA+AGG 0.582193 3.2:+90076419 MS.gene42277:CDS
CTTACCAACATCATCATCCA+AGG 0.591615 3.2:-90076299 None:intergenic
AATACCTTGGATGATGATGT+TGG 0.652086 3.2:+90076295 MS.gene42277:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTAAGAAATTGAATAG+AGG + chr3.2:90075953-90075972 MS.gene42277:CDS 20.0%
!! ATTAGAATAGGATTTGATAT+TGG + chr3.2:90076088-90076107 MS.gene42277:CDS 20.0%
!! TCAAAGACATTATCATAAAA+AGG - chr3.2:90076259-90076278 None:intergenic 20.0%
!!! TATATGAATTACAAAGTGTT+TGG + chr3.2:90075731-90075750 MS.gene42277:CDS 20.0%
!!! TTATGATAATGTCTTTGATT+TGG + chr3.2:90076261-90076280 MS.gene42277:CDS 20.0%
! AGAAGAAAATTTCATTGACA+AGG + chr3.2:90076419-90076438 MS.gene42277:CDS 25.0%
! ATAGGATTTGATATTGGAAT+TGG + chr3.2:90076094-90076113 MS.gene42277:CDS 25.0%
! TGGATACAGAAAATTGAAAT+GGG + chr3.2:90076456-90076475 MS.gene42277:CDS 25.0%
! TTGGATACAGAAAATTGAAA+TGG + chr3.2:90076455-90076474 MS.gene42277:CDS 25.0%
!! AAAAAGATTGGTTGTGATAA+CGG - chr3.2:90075387-90075406 None:intergenic 25.0%
!!! CTCAGAAATTGATTTTGAAA+GGG + chr3.2:90075780-90075799 MS.gene42277:CDS 25.0%
AATGAATTTATAGCTGCAAG+AGG + chr3.2:90076196-90076215 MS.gene42277:CDS 30.0%
AGCATAAAGAGCAAAAAGAT+TGG - chr3.2:90075399-90075418 None:intergenic 30.0%
GCAGCTATAAATTCATTGAA+AGG - chr3.2:90076193-90076212 None:intergenic 30.0%
TGAATGGTTATGAGAATCAA+AGG + chr3.2:90075999-90076018 MS.gene42277:CDS 30.0%
TTCCAAAGTGAATTTGGAAA+AGG - chr3.2:90075872-90075891 None:intergenic 30.0%
! ATCCTTTTCCAAATTCACTT+TGG + chr3.2:90075867-90075886 MS.gene42277:CDS 30.0%
! GGTTCTGTTTCTTTGAAAAT+TGG + chr3.2:90075293-90075312 MS.gene42277:CDS 30.0%
! GTTTCTTTGAAAATTGGTGA+TGG + chr3.2:90075299-90075318 MS.gene42277:CDS 30.0%
! TGCTAAATCTGTTTCTTCTA+AGG + chr3.2:90075832-90075851 MS.gene42277:CDS 30.0%
! TTAAGCTCTTTTGCAATGTT+GGG - chr3.2:90075596-90075615 None:intergenic 30.0%
! TTGGTAACAAGACTGTTAAT+TGG + chr3.2:90075897-90075916 MS.gene42277:CDS 30.0%
! TTTAAGCTCTTTTGCAATGT+TGG - chr3.2:90075597-90075616 None:intergenic 30.0%
! TTTGATATTGGAATTGGTTC+TGG + chr3.2:90076100-90076119 MS.gene42277:CDS 30.0%
! TTTTCCTCTTTTCTTAAGCT+TGG + chr3.2:90076222-90076241 MS.gene42277:CDS 30.0%
! TTTTCTTAAGCTTGGATCAT+AGG + chr3.2:90076230-90076249 MS.gene42277:CDS 30.0%
!! AGAAAATTGAAATGGGTTGT+GGG + chr3.2:90076463-90076482 MS.gene42277:CDS 30.0%
!! GCTCAGAAATTGATTTTGAA+AGG + chr3.2:90075779-90075798 MS.gene42277:CDS 30.0%
!! TTTTCTTGCTGATGATGTTT+TGG + chr3.2:90076048-90076067 MS.gene42277:CDS 30.0%
!!! AAGAAGCTGTTTTTCTATCT+GGG - chr3.2:90075546-90075565 None:intergenic 30.0%
AAACACCTTCTTCTAGGTAA+AGG - chr3.2:90075812-90075831 None:intergenic 35.0%
AACAAGACTGTTAATTGGAG+TGG + chr3.2:90075902-90075921 MS.gene42277:CDS 35.0%
AACCCTCATAAACTTCCTTT+GGG + chr3.2:90075626-90075645 MS.gene42277:CDS 35.0%
ACAGGTTTCCAAAGTGAATT+TGG - chr3.2:90075878-90075897 None:intergenic 35.0%
ACTGAAATGGTTTCATCTGT+GGG + chr3.2:90075671-90075690 MS.gene42277:CDS 35.0%
CAAAACTGGAATCACTGAAA+TGG + chr3.2:90075658-90075677 MS.gene42277:CDS 35.0%
CAGATAGAAAAACAGCTTCT+TGG + chr3.2:90075545-90075564 MS.gene42277:CDS 35.0%
TAACCCTCATAAACTTCCTT+TGG + chr3.2:90075625-90075644 MS.gene42277:CDS 35.0%
TACCCAAAGGAAGTTTATGA+GGG - chr3.2:90075631-90075650 None:intergenic 35.0%
TGATCCAAGCTTAAGAAAAG+AGG - chr3.2:90076229-90076248 None:intergenic 35.0%
TGGTAAGAAACAAGAGAAGT+TGG + chr3.2:90076315-90076334 MS.gene42277:CDS 35.0%
TTACCCAAAGGAAGTTTATG+AGG - chr3.2:90075632-90075651 None:intergenic 35.0%
TTAGCAAAACACCTTCTTCT+AGG - chr3.2:90075818-90075837 None:intergenic 35.0%
TTGCATGCATTGCTTTGATT+TGG + chr3.2:90075976-90075995 MS.gene42277:CDS 35.0%
TTGGAAATTGTCAGGATGAT+TGG + chr3.2:90075750-90075769 MS.gene42277:CDS 35.0%
TTGGGTAAAGATTCCAAAAC+TGG + chr3.2:90075644-90075663 MS.gene42277:CDS 35.0%
! AATACCTTGGATGATGATGT+TGG + chr3.2:90076295-90076314 MS.gene42277:CDS 35.0%
! AATTCACTTTGGAAACCTGT+TGG + chr3.2:90075878-90075897 MS.gene42277:CDS 35.0%
! ACAAGGTTGATTGAAAGGTT+TGG + chr3.2:90076436-90076455 MS.gene42277:CDS 35.0%
! CAAAGTGTTTGGAAATTGTC+AGG + chr3.2:90075742-90075761 MS.gene42277:CDS 35.0%
! CATTGACAAGGTTGATTGAA+AGG + chr3.2:90076431-90076450 MS.gene42277:CDS 35.0%
! CATTTCAGTGATTCCAGTTT+TGG - chr3.2:90075660-90075679 None:intergenic 35.0%
! GAGAGAAGTTTTTGTGTACT+TGG - chr3.2:90075463-90075482 None:intergenic 35.0%
! GGAAATGGTGGAATTAGAAT+AGG + chr3.2:90076076-90076095 MS.gene42277:CDS 35.0%
!! CAGAAAATTGAAATGGGTTG+TGG + chr3.2:90076462-90076481 MS.gene42277:CDS 35.0%
!! CATTGCTTTGATTTGGTGAA+TGG + chr3.2:90075983-90076002 MS.gene42277:CDS 35.0%
!! TTAACAGTCTTGTTACCAAC+AGG - chr3.2:90075896-90075915 None:intergenic 35.0%
!!! CAAGAAGCTGTTTTTCTATC+TGG - chr3.2:90075547-90075566 None:intergenic 35.0%
!!! GATGTTTTGGCATTAGGAAA+TGG + chr3.2:90076061-90076080 MS.gene42277:CDS 35.0%
!!! GTTTTGGAATCTTTACCCAA+AGG - chr3.2:90075644-90075663 None:intergenic 35.0%
CACTGAAATGGTTTCATCTG+TGG + chr3.2:90075670-90075689 MS.gene42277:CDS 40.0%
CTTACCAACATCATCATCCA+AGG - chr3.2:90076302-90076321 None:intergenic 40.0%
TGTTCTTCAGAAGCCTGTTA+GGG + chr3.2:90076528-90076547 MS.gene42277:CDS 40.0%
! GTGTACTTGGTGAAGTTGTT+CGG - chr3.2:90075450-90075469 None:intergenic 40.0%
! TCGACATCGATCGAATTTTG+CGG + chr3.2:90076350-90076369 MS.gene42277:CDS 40.0%
! TGGATCTTTTGCTGCTGTAA+TGG + chr3.2:90076120-90076139 MS.gene42277:CDS 40.0%
!! GTTTTGGCATTAGGAAATGG+TGG + chr3.2:90076064-90076083 MS.gene42277:CDS 40.0%
!!! GCTGATGATGTTTTGGCATT+AGG + chr3.2:90076055-90076074 MS.gene42277:CDS 40.0%
!!! GGGCTAGTTTCTTTTGAGAT+TGG - chr3.2:90075526-90075545 None:intergenic 40.0%
!!! TGTTCGGTTTGGCTGTTTTT+TGG - chr3.2:90075434-90075453 None:intergenic 40.0%
CTGTTCTTCAGAAGCCTGTT+AGG + chr3.2:90076527-90076546 MS.gene42277:CDS 45.0%
CTTGGTGAAGTTGTTCGGTT+TGG - chr3.2:90075445-90075464 None:intergenic 45.0%
GGTTCGTGCTACAAATACCT+TGG + chr3.2:90076282-90076301 MS.gene42277:CDS 45.0%
GTGAGCCTTTACCTAGAAGA+AGG + chr3.2:90075804-90075823 MS.gene42277:CDS 45.0%
TTGCTGCTGTAATGGCTGAA+AGG + chr3.2:90076128-90076147 MS.gene42277:CDS 45.0%
! CGACATCGATCGAATTTTGC+GGG + chr3.2:90076351-90076370 MS.gene42277:CDS 45.0%
!! GAAATGGGTTGTGGGAGAAA+AGG + chr3.2:90076471-90076490 MS.gene42277:CDS 45.0%
!! GTGGGAGAAAAGGTTGATAG+TGG + chr3.2:90076481-90076500 MS.gene42277:CDS 45.0%
! ATCGATCGAATTTTGCGGGC+TGG + chr3.2:90076355-90076374 MS.gene42277:CDS 50.0%
!!! GATCGAATTTTGCGGGCTGG+TGG + chr3.2:90076358-90076377 MS.gene42277:CDS 55.0%
!!! TGCGGGCTGGTGGTTTGTTT+TGG + chr3.2:90076368-90076387 MS.gene42277:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 90075290 90076555 90075290 ID=MS.gene42277
chr3.2 mRNA 90075290 90076555 90075290 ID=MS.gene42277.t1;Parent=MS.gene42277
chr3.2 exon 90075290 90076555 90075290 ID=MS.gene42277.t1.exon1;Parent=MS.gene42277.t1
chr3.2 CDS 90075290 90076555 90075290 ID=cds.MS.gene42277.t1;Parent=MS.gene42277.t1
Gene Sequence

>MS.gene42277

ATGGGTTCTGTTTCTTTGAAAATTGGTGATGGAACAGCAAGATTCAAAAAATCAACACTTTGTTCTTCAGCTGTTAACATTCTCATGATCTTATCCGTTATCACAACCAATCTTTTTGCTCTTTATGCTTTCACTTCTTCTCCAAAAAACAGCCAAACCGAACAACTTCACCAAGTACACAAAAACTTCTCTCTCATCTCAGAACAAGTTTCACTTATACTCAGAGAAATTGACCAATCTCAAAAGAAACTAGCCCAGATAGAAAAACAGCTTCTTGGCTATGAAAGTTTTGATCTTTCAAGACCCAACATTGCAAAAGAGCTTAAACTCTTTCTTAACCCTCATAAACTTCCTTTGGGTAAAGATTCCAAAACTGGAATCACTGAAATGGTTTCATCTGTGGGTCATTCTTGTGAGAAATCTTCTGATTTGTTGTCTCAGTATATGAATTACAAAGTGTTTGGAAATTGTCAGGATGATTGGAGTTATGCTCAGAAATTGATTTTGAAAGGGTGTGAGCCTTTACCTAGAAGAAGGTGTTTTGCTAAATCTGTTTCTTCTAAGGTAAATTATCTTCATCCTTTTCCAAATTCACTTTGGAAACCTGTTGGTAACAAGACTGTTAATTGGAGTGGTTTTAGTTGTAAGAATTTTGAGTGTTTGAATGTTAAGAAATTGAATAGAGGTTGCATGCATTGCTTTGATTTGGTGAATGGTTATGAGAATCAAAGGTTTGTTAAGTCTAGAAGTAAGAATGATTTTCTTGCTGATGATGTTTTGGCATTAGGAAATGGTGGAATTAGAATAGGATTTGATATTGGAATTGGTTCTGGATCTTTTGCTGCTGTAATGGCTGAAAGGAATGTTACAATTGTTACTAGTACTCTTAATATTGATGCTCCTTTCAATGAATTTATAGCTGCAAGAGGACTTTTTCCTCTTTTCTTAAGCTTGGATCATAGGTTTCCTTTTTATGATAATGTCTTTGATTTGGTTCGTGCTACAAATACCTTGGATGATGATGTTGGTAAGAAACAAGAGAAGTTGGAGTTTTTAATGTTCGACATCGATCGAATTTTGCGGGCTGGTGGTTTGTTTTGGATTGATAACTTCTATTGTGCTAATGATGAGAAGAAAATTTCATTGACAAGGTTGATTGAAAGGTTTGGATACAGAAAATTGAAATGGGTTGTGGGAGAAAAGGTTGATAGTGGTAAGTCTCAAGTTTTCTTATCTGCTGTTCTTCAGAAGCCTGTTAGGGTGTAA

Protein sequence

>MS.gene42277.t1

MGSVSLKIGDGTARFKKSTLCSSAVNILMILSVITTNLFALYAFTSSPKNSQTEQLHQVHKNFSLISEQVSLILREIDQSQKKLAQIEKQLLGYESFDLSRPNIAKELKLFLNPHKLPLGKDSKTGITEMVSSVGHSCEKSSDLLSQYMNYKVFGNCQDDWSYAQKLILKGCEPLPRRRCFAKSVSSKVNYLHPFPNSLWKPVGNKTVNWSGFSCKNFECLNVKKLNRGCMHCFDLVNGYENQRFVKSRSKNDFLADDVLALGNGGIRIGFDIGIGSGSFAAVMAERNVTIVTSTLNIDAPFNEFIAARGLFPLFLSLDHRFPFYDNVFDLVRATNTLDDDVGKKQEKLEFLMFDIDRILRAGGLFWIDNFYCANDEKKISLTRLIERFGYRKLKWVVGEKVDSGKSQVFLSAVLQKPVRV