Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36865.t1 | XP_013444909.1 | 87.6 | 178 | 22 | 0 | 1 | 178 | 1 | 178 | 2.80E-82 | 314.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36865.t1 | Q58FX0 | 35.0 | 203 | 98 | 6 | 3 | 176 | 8 | 205 | 1.8e-21 | 104.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36865.t1 | I3SZG8 | 87.6 | 178 | 22 | 0 | 1 | 178 | 1 | 178 | 2.0e-82 | 314.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049413 | MS.gene36865 | 0.802667 | 5.03E-49 | -1.69E-46 |
| MS.gene04998 | MS.gene36865 | 0.801337 | 9.44E-49 | -1.69E-46 |
| MS.gene050961 | MS.gene36865 | 0.805693 | 1.18E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene36865 | 0.806625 | 7.51E-50 | -1.69E-46 |
| MS.gene054892 | MS.gene36865 | 0.866487 | 2.75E-65 | -1.69E-46 |
| MS.gene054893 | MS.gene36865 | 0.858121 | 1.02E-62 | -1.69E-46 |
| MS.gene05861 | MS.gene36865 | 0.817342 | 3.47E-52 | -1.69E-46 |
| MS.gene05969 | MS.gene36865 | 0.815683 | 8.16E-52 | -1.69E-46 |
| MS.gene059774 | MS.gene36865 | 0.809929 | 1.48E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene36865 | 0.805474 | 1.31E-49 | -1.69E-46 |
| MS.gene060697 | MS.gene36865 | 0.809019 | 2.33E-50 | -1.69E-46 |
| MS.gene061047 | MS.gene36865 | 0.849755 | 2.64E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36865.t1 | MTR_8g432370 | 90.449 | 178 | 17 | 0 | 1 | 178 | 1 | 178 | 1.80e-110 | 312 |
| MS.gene36865.t1 | MTR_8g432390 | 91.620 | 179 | 14 | 1 | 1 | 178 | 1 | 179 | 2.50e-110 | 312 |
| MS.gene36865.t1 | MTR_8g432350 | 89.944 | 179 | 17 | 1 | 1 | 178 | 1 | 179 | 8.93e-109 | 308 |
| MS.gene36865.t1 | MTR_8g432400 | 89.385 | 179 | 18 | 1 | 1 | 178 | 1 | 179 | 2.77e-106 | 302 |
| MS.gene36865.t1 | MTR_7g076000 | 51.934 | 181 | 66 | 6 | 1 | 176 | 1 | 165 | 1.92e-51 | 162 |
| MS.gene36865.t1 | MTR_8g432470 | 37.705 | 183 | 97 | 5 | 2 | 174 | 5 | 180 | 1.19e-28 | 105 |
| MS.gene36865.t1 | MTR_8g432440 | 36.612 | 183 | 99 | 5 | 2 | 174 | 5 | 180 | 2.06e-28 | 104 |
| MS.gene36865.t1 | MTR_8g432420 | 36.612 | 183 | 99 | 5 | 2 | 174 | 5 | 180 | 2.06e-28 | 104 |
| MS.gene36865.t1 | MTR_8g432450 | 36.158 | 177 | 98 | 3 | 2 | 168 | 5 | 176 | 2.54e-27 | 101 |
| MS.gene36865.t1 | MTR_8g432460 | 35.714 | 182 | 101 | 5 | 2 | 174 | 5 | 179 | 7.71e-25 | 95.5 |
| MS.gene36865.t1 | MTR_8g432490 | 34.615 | 182 | 103 | 5 | 2 | 174 | 5 | 179 | 3.02e-23 | 91.3 |
| MS.gene36865.t1 | MTR_3g064780 | 34.066 | 182 | 104 | 5 | 2 | 174 | 5 | 179 | 3.54e-22 | 89.7 |
| MS.gene36865.t1 | MTR_8g432480 | 33.735 | 166 | 96 | 4 | 2 | 156 | 7 | 169 | 1.03e-21 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36865.t1 | AT2G45760 | 35.122 | 205 | 99 | 6 | 1 | 176 | 6 | 205 | 1.20e-31 | 113 |
| MS.gene36865.t1 | AT3G61190 | 36.612 | 183 | 105 | 6 | 1 | 178 | 16 | 192 | 9.03e-27 | 100 |
| MS.gene36865.t1 | AT3G61190 | 36.612 | 183 | 105 | 6 | 1 | 178 | 16 | 192 | 9.03e-27 | 100 |
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAACAACAAATCCATAAA+AGG | 0.232293 | 8.4:-64154222 | MS.gene36865:CDS |
| CACCCTTTCAAATGTGTCTT+TGG | 0.267837 | 8.4:+64153749 | None:intergenic |
| TTATGGATTTGTTGTTCTCT+TGG | 0.307187 | 8.4:+64154226 | None:intergenic |
| ATAATAGAGTTAGGAGAAAT+GGG | 0.321912 | 8.4:-64153933 | MS.gene36865:CDS |
| GATAATAGAGTTAGGAGAAA+TGG | 0.337385 | 8.4:-64153934 | MS.gene36865:CDS |
| CAGCAGGGATTCCAGCTGTT+TGG | 0.347843 | 8.4:-64153780 | MS.gene36865:CDS |
| GGCGTTCCGGTGGCCGGTAA+TGG | 0.366500 | 8.4:-64153820 | MS.gene36865:CDS |
| GTGAATGGAGTTCCGGTAAC+TGG | 0.395156 | 8.4:-64153841 | MS.gene36865:CDS |
| AATTAGAACAAGAACTTTGT+TGG | 0.400854 | 8.4:+64153878 | None:intergenic |
| GTACTTCACCTCTATTGTTA+CGG | 0.407402 | 8.4:+64154068 | None:intergenic |
| TACATACCCTCCAAGAAAAT+CGG | 0.418731 | 8.4:+64153996 | None:intergenic |
| CGTAGGGTAACTTCCGCCTT+CGG | 0.421093 | 8.4:+64154134 | None:intergenic |
| ATTAGAACAAGAACTTTGTT+GGG | 0.425451 | 8.4:+64153879 | None:intergenic |
| AAGTAGTAGTAGTGTTGGTA+TGG | 0.432057 | 8.4:-64154034 | MS.gene36865:CDS |
| GTTACGGACCTTGCATGTAA+TGG | 0.432977 | 8.4:+64154084 | None:intergenic |
| CCCTGCTGCAACTCTGCTGC+AGG | 0.437005 | 8.4:+64153795 | None:intergenic |
| TCCTGCAGCAGAGTTGCAGC+AGG | 0.439575 | 8.4:-64153796 | MS.gene36865:CDS |
| TCTGCTGCAGGAGCCATTAC+CGG | 0.444058 | 8.4:+64153807 | None:intergenic |
| GCCACCGGAACGCCAGTTAC+CGG | 0.450510 | 8.4:+64153829 | None:intergenic |
| AGTTCCGGTAACTGGCGTTC+CGG | 0.456993 | 8.4:-64153833 | MS.gene36865:CDS |
| GGTTGTGGGATAATAGAGTT+AGG | 0.466052 | 8.4:-64153942 | MS.gene36865:CDS |
| TGGTAGTTTAACCAAACAGC+TGG | 0.466544 | 8.4:+64153769 | None:intergenic |
| AGAGGAAGTAGTAGTAGTGT+TGG | 0.485422 | 8.4:-64154039 | MS.gene36865:CDS |
| GCCTTCGGAATCAAGTTTCG+TGG | 0.489360 | 8.4:+64154149 | None:intergenic |
| TCAATGTCATCGTCGGTGAA+TGG | 0.489891 | 8.4:-64153856 | MS.gene36865:CDS |
| GTAACTGGCGTTCCGGTGGC+CGG | 0.501861 | 8.4:-64153826 | MS.gene36865:CDS |
| AGAACAACAAATCCATAAAA+GGG | 0.503622 | 8.4:-64154221 | MS.gene36865:CDS |
| TGTTGGTATGGCTAGAATAC+CGG | 0.506863 | 8.4:-64154022 | MS.gene36865:CDS |
| ATCGTCGGTGAATGGAGTTC+CGG | 0.515963 | 8.4:-64153848 | MS.gene36865:CDS |
| CCTCCAAGAAAATCGGAAAC+CGG | 0.520232 | 8.4:+64154003 | None:intergenic |
| TGCAAGGTCCGTAACAATAG+AGG | 0.524203 | 8.4:-64154076 | MS.gene36865:CDS |
| TTCTAATTCAATGTCATCGT+CGG | 0.527140 | 8.4:-64153863 | MS.gene36865:CDS |
| CAGGAGCCATTACCGGCCAC+CGG | 0.541843 | 8.4:+64153814 | None:intergenic |
| TCCGGTAACTGGCGTTCCGG+TGG | 0.553121 | 8.4:-64153830 | MS.gene36865:CDS |
| CCCTACGTGGAACGAGAAGT+TGG | 0.563010 | 8.4:-64154118 | MS.gene36865:CDS |
| CCAACTTCTCGTTCCACGTA+GGG | 0.563124 | 8.4:+64154118 | None:intergenic |
| TGGGGTTGTAAATATTTCAG+TGG | 0.590455 | 8.4:-64153914 | MS.gene36865:CDS |
| TTTGCCACTGTTCAATGCGA+CGG | 0.591761 | 8.4:-64154192 | MS.gene36865:CDS |
| CCTGCAGCAGAGTTGCAGCA+GGG | 0.618156 | 8.4:-64153795 | MS.gene36865:CDS |
| GAGGTGAAGTACAAAACTAG+AGG | 0.629180 | 8.4:-64154057 | MS.gene36865:CDS |
| ACGAAACTTGATTCCGAAGG+CGG | 0.631770 | 8.4:-64154147 | MS.gene36865:CDS |
| TAATAGAGTTAGGAGAAATG+GGG | 0.635926 | 8.4:-64153932 | MS.gene36865:CDS |
| TCGACGTGCCATTACATGCA+AGG | 0.639724 | 8.4:-64154092 | MS.gene36865:CDS |
| ACCACGAAACTTGATTCCGA+AGG | 0.642396 | 8.4:-64154150 | MS.gene36865:CDS |
| GCTTCCGTCGCATTGAACAG+TGG | 0.660173 | 8.4:+64154188 | None:intergenic |
| AAGGCGGAAGTTACCCTACG+TGG | 0.698408 | 8.4:-64154131 | MS.gene36865:CDS |
| ACCAACTTCTCGTTCCACGT+AGG | 0.720845 | 8.4:+64154117 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTGCAATTTTTAAGTTAT+AGG | - | chr8.4:64154063-64154082 | MS.gene36865:CDS | 15.0% |
| !!! | AATTTTTAAGTTATAGGTTG+TGG | - | chr8.4:64154069-64154088 | MS.gene36865:CDS | 20.0% |
| !!! | ATTTTTAAGTTATAGGTTGT+GGG | - | chr8.4:64154070-64154089 | MS.gene36865:CDS | 20.0% |
| ! | AATTAGAACAAGAACTTTGT+TGG | + | chr8.4:64154151-64154170 | None:intergenic | 25.0% |
| ! | AGAACAACAAATCCATAAAA+GGG | - | chr8.4:64153805-64153824 | MS.gene36865:CDS | 25.0% |
| ! | ATAATAGAGTTAGGAGAAAT+GGG | - | chr8.4:64154093-64154112 | MS.gene36865:CDS | 25.0% |
| ! | ATTAGAACAAGAACTTTGTT+GGG | + | chr8.4:64154150-64154169 | None:intergenic | 25.0% |
| GAGAACAACAAATCCATAAA+AGG | - | chr8.4:64153804-64153823 | MS.gene36865:CDS | 30.0% | |
| GATAATAGAGTTAGGAGAAA+TGG | - | chr8.4:64154092-64154111 | MS.gene36865:CDS | 30.0% | |
| TAATAGAGTTAGGAGAAATG+GGG | - | chr8.4:64154094-64154113 | MS.gene36865:CDS | 30.0% | |
| TTCTAATTCAATGTCATCGT+CGG | - | chr8.4:64154163-64154182 | MS.gene36865:CDS | 30.0% | |
| ! | AGAGATAACTATTTTGTCAG+CGG | - | chr8.4:64153770-64153789 | MS.gene36865:CDS | 30.0% |
| !! | TTATGGATTTGTTGTTCTCT+TGG | + | chr8.4:64153803-64153822 | None:intergenic | 30.0% |
| GTACTTCACCTCTATTGTTA+CGG | + | chr8.4:64153961-64153980 | None:intergenic | 35.0% | |
| TACATACCCTCCAAGAAAAT+CGG | + | chr8.4:64154033-64154052 | None:intergenic | 35.0% | |
| TGGGGTTGTAAATATTTCAG+TGG | - | chr8.4:64154112-64154131 | MS.gene36865:CDS | 35.0% | |
| !! | AAGTAGTAGTAGTGTTGGTA+TGG | - | chr8.4:64153992-64154011 | MS.gene36865:CDS | 35.0% |
| AGAGGAAGTAGTAGTAGTGT+TGG | - | chr8.4:64153987-64154006 | MS.gene36865:CDS | 40.0% | |
| GAGGTGAAGTACAAAACTAG+AGG | - | chr8.4:64153969-64153988 | MS.gene36865:CDS | 40.0% | |
| GGTTGTGGGATAATAGAGTT+AGG | - | chr8.4:64154084-64154103 | MS.gene36865:CDS | 40.0% | |
| TGGTAGTTTAACCAAACAGC+TGG | + | chr8.4:64154260-64154279 | None:intergenic | 40.0% | |
| TGTTGGTATGGCTAGAATAC+CGG | - | chr8.4:64154004-64154023 | MS.gene36865:CDS | 40.0% | |
| ! | GGCAAATGTGTTCCCTTTTA+TGG | + | chr8.4:64153820-64153839 | None:intergenic | 40.0% |
| !!! | ATACCGGTTTCCGATTTTCT+TGG | - | chr8.4:64154020-64154039 | MS.gene36865:CDS | 40.0% |
| ACCACGAAACTTGATTCCGA+AGG | - | chr8.4:64153876-64153895 | MS.gene36865:CDS | 45.0% | |
| ACGAAACTTGATTCCGAAGG+CGG | - | chr8.4:64153879-64153898 | MS.gene36865:CDS | 45.0% | |
| CCTCCAAGAAAATCGGAAAC+CGG | + | chr8.4:64154026-64154045 | None:intergenic | 45.0% | |
| GTTACGGACCTTGCATGTAA+TGG | + | chr8.4:64153945-64153964 | None:intergenic | 45.0% | |
| TCAATGTCATCGTCGGTGAA+TGG | - | chr8.4:64154170-64154189 | MS.gene36865:CDS | 45.0% | |
| TGCAAGGTCCGTAACAATAG+AGG | - | chr8.4:64153950-64153969 | MS.gene36865:CDS | 45.0% | |
| TTTGCCACTGTTCAATGCGA+CGG | - | chr8.4:64153834-64153853 | MS.gene36865:CDS | 45.0% | |
| ! | CGGTTTCCGATTTTCTTGGA+GGG | - | chr8.4:64154024-64154043 | MS.gene36865:CDS | 45.0% |
| ACCAACTTCTCGTTCCACGT+AGG | + | chr8.4:64153912-64153931 | None:intergenic | 50.0% | |
| ATCGTCGGTGAATGGAGTTC+CGG | - | chr8.4:64154178-64154197 | MS.gene36865:CDS | 50.0% | |
| CCAACTTCTCGTTCCACGTA+GGG | + | chr8.4:64153911-64153930 | None:intergenic | 50.0% | |
| GCCTTCGGAATCAAGTTTCG+TGG | + | chr8.4:64153880-64153899 | None:intergenic | 50.0% | |
| GTGAATGGAGTTCCGGTAAC+TGG | - | chr8.4:64154185-64154204 | MS.gene36865:CDS | 50.0% | |
| ! | CCGGTTTCCGATTTTCTTGG+AGG | - | chr8.4:64154023-64154042 | MS.gene36865:CDS | 50.0% |
| ! | TCGACGTGCCATTACATGCA+AGG | - | chr8.4:64153934-64153953 | MS.gene36865:CDS | 50.0% |
| AAGGCGGAAGTTACCCTACG+TGG | - | chr8.4:64153895-64153914 | MS.gene36865:CDS | 55.0% | |
| AGTTCCGGTAACTGGCGTTC+CGG | - | chr8.4:64154193-64154212 | MS.gene36865:CDS | 55.0% | |
| CAGCAGGGATTCCAGCTGTT+TGG | - | chr8.4:64154246-64154265 | MS.gene36865:CDS | 55.0% | |
| CCCTACGTGGAACGAGAAGT+TGG | - | chr8.4:64153908-64153927 | MS.gene36865:CDS | 55.0% | |
| CGTAGGGTAACTTCCGCCTT+CGG | + | chr8.4:64153895-64153914 | None:intergenic | 55.0% | |
| GCTTCCGTCGCATTGAACAG+TGG | + | chr8.4:64153841-64153860 | None:intergenic | 55.0% | |
| TCTGCTGCAGGAGCCATTAC+CGG | + | chr8.4:64154222-64154241 | None:intergenic | 55.0% | |
| CCTGCAGCAGAGTTGCAGCA+GGG | - | chr8.4:64154231-64154250 | MS.gene36865:CDS | 60.0% | |
| TCCTGCAGCAGAGTTGCAGC+AGG | - | chr8.4:64154230-64154249 | MS.gene36865:CDS | 60.0% | |
| CAGGAGCCATTACCGGCCAC+CGG | + | chr8.4:64154215-64154234 | None:intergenic | 65.0% | |
| CCCTGCTGCAACTCTGCTGC+AGG | + | chr8.4:64154234-64154253 | None:intergenic | 65.0% | |
| GCCACCGGAACGCCAGTTAC+CGG | + | chr8.4:64154200-64154219 | None:intergenic | 65.0% | |
| GTAACTGGCGTTCCGGTGGC+CGG | - | chr8.4:64154200-64154219 | MS.gene36865:CDS | 65.0% | |
| TCCGGTAACTGGCGTTCCGG+TGG | - | chr8.4:64154196-64154215 | MS.gene36865:CDS | 65.0% | |
| GGCGTTCCGGTGGCCGGTAA+TGG | - | chr8.4:64154206-64154225 | MS.gene36865:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 64153756 | 64154292 | 64153756 | ID=MS.gene36865 |
| chr8.4 | mRNA | 64153756 | 64154292 | 64153756 | ID=MS.gene36865.t1;Parent=MS.gene36865 |
| chr8.4 | exon | 64153756 | 64154292 | 64153756 | ID=MS.gene36865.t1.exon1;Parent=MS.gene36865.t1 |
| chr8.4 | CDS | 64153756 | 64154292 | 64153756 | ID=cds.MS.gene36865.t1;Parent=MS.gene36865.t1 |
| Gene Sequence |
| Protein sequence |
