Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37637.t1 | TKY46131.1 | 93.6 | 109 | 7 | 0 | 1 | 109 | 80 | 188 | 2.30E-55 | 224.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37637.t1 | Q9FJI1 | 69.8 | 106 | 32 | 0 | 1 | 106 | 71 | 176 | 5.3e-40 | 164.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37637.t1 | A0A392PUY8 | 95.5 | 110 | 5 | 0 | 1 | 110 | 1 | 110 | 5.7e-56 | 226.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050277 | MS.gene37637 | 0.807721 | 4.40E-50 | -1.69E-46 |
| MS.gene050317 | MS.gene37637 | 0.808352 | 3.23E-50 | -1.69E-46 |
| MS.gene050320 | MS.gene37637 | 0.827385 | 1.61E-54 | -1.69E-46 |
| MS.gene051063 | MS.gene37637 | 0.811576 | 6.53E-51 | -1.69E-46 |
| MS.gene051451 | MS.gene37637 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene051746 | MS.gene37637 | 0.802082 | 6.64E-49 | -1.69E-46 |
| MS.gene051748 | MS.gene37637 | 0.812541 | 4.02E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene37637 | 0.812361 | 4.41E-51 | -1.69E-46 |
| MS.gene053109 | MS.gene37637 | 0.840944 | 6.41E-58 | -1.69E-46 |
| MS.gene056432 | MS.gene37637 | 0.802608 | 5.17E-49 | -1.69E-46 |
| MS.gene057094 | MS.gene37637 | 0.834987 | 2.18E-56 | -1.69E-46 |
| MS.gene057234 | MS.gene37637 | 0.821783 | 3.36E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene37637 | 0.804641 | 1.96E-49 | -1.69E-46 |
| MS.gene058238 | MS.gene37637 | 0.803322 | 3.68E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene37637 | 0.809341 | 1.99E-50 | -1.69E-46 |
| MS.gene06001 | MS.gene37637 | 0.818383 | 2.02E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37637.t1 | MTR_5g087910 | 96.364 | 110 | 4 | 0 | 1 | 110 | 68 | 177 | 7.99e-75 | 219 |
| MS.gene37637.t1 | MTR_5g087910 | 96.364 | 110 | 4 | 0 | 1 | 110 | 140 | 249 | 1.45e-73 | 218 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37637.t1 | AT5G60340 | 69.811 | 106 | 32 | 0 | 1 | 106 | 71 | 176 | 1.38e-51 | 160 |
Find 24 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCAGACGGTCATACAAAA+TGG | 0.376389 | 5.3:-70714244 | None:intergenic |
| GATTTCACATTCGACGTTGT+TGG | 0.388413 | 5.3:-70714391 | None:intergenic |
| TGATGAACTTGAGGATGTTA+TGG | 0.431142 | 5.3:+70714131 | MS.gene37637:CDS |
| GAATTGGCCCATCCCAGTAT+AGG | 0.434368 | 5.3:+70714533 | MS.gene37637:CDS |
| TGACAGATTGGATCAGGAAT+TGG | 0.437273 | 5.3:+70714517 | MS.gene37637:CDS |
| AATTGGCCCATCCCAGTATA+GGG | 0.447984 | 5.3:+70714534 | MS.gene37637:CDS |
| CAACTCTGACAGATTGGATC+AGG | 0.470384 | 5.3:+70714511 | MS.gene37637:CDS |
| GGGAATATTGTGGACTATCA+TGG | 0.474310 | 5.3:+70714162 | MS.gene37637:CDS |
| GTGATTTCTTTCCCGAGCGA+TGG | 0.476262 | 5.3:+70714187 | MS.gene37637:CDS |
| ACTTGAGGATGTTATGGAAG+AGG | 0.497760 | 5.3:+70714137 | MS.gene37637:CDS |
| CGAGCGATGGTTTGATTGTG+TGG | 0.514486 | 5.3:+70714200 | MS.gene37637:CDS |
| ATGTTGCAACTCTGACAGAT+TGG | 0.522799 | 5.3:+70714505 | MS.gene37637:CDS |
| CTTGAGGATGTTATGGAAGA+GGG | 0.525425 | 5.3:+70714138 | MS.gene37637:CDS |
| CACACAATCAAACCATCGCT+CGG | 0.528082 | 5.3:-70714199 | None:intergenic |
| GGAAGAGGGAGGGAATATTG+TGG | 0.540361 | 5.3:+70714152 | MS.gene37637:CDS |
| AAAATACCCTATACTGGGAT+GGG | 0.545741 | 5.3:-70714540 | None:intergenic |
| GAGGATGTTATGGAAGAGGG+AGG | 0.559657 | 5.3:+70714141 | MS.gene37637:CDS |
| TGTATGACCGTCTGAGCAGA+AGG | 0.560928 | 5.3:+70714250 | MS.gene37637:CDS |
| ACACAATCAAACCATCGCTC+GGG | 0.566646 | 5.3:-70714198 | None:intergenic |
| GTAGGTGTGTGATGAACTTG+AGG | 0.579640 | 5.3:+70714122 | None:intergenic |
| TGACATACCTTCTGCTCAGA+CGG | 0.610039 | 5.3:-70714257 | None:intergenic |
| AGGATGTTATGGAAGAGGGA+GGG | 0.612954 | 5.3:+70714142 | MS.gene37637:CDS |
| GGCTAAAGAAAGTTACGCGG+AGG | 0.677847 | 5.3:+70714434 | MS.gene37637:CDS |
| GGAGGCTAAAGAAAGTTACG+CGG | 0.771874 | 5.3:+70714431 | MS.gene37637:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GCAATTCTATTGTATATTTC+AGG | + | chr5.3:70714311-70714330 | MS.gene37637:intron | 25.0% |
| !!! | AATCTTTCAAGTTTTGCTTG+AGG | + | chr5.3:70714410-70714429 | MS.gene37637:CDS | 30.0% |
| ACTTTATTCATCTCTGTGAC+AGG | + | chr5.3:70714346-70714365 | MS.gene37637:intron | 35.0% | |
| CTTTATTCATCTCTGTGACA+GGG | + | chr5.3:70714347-70714366 | MS.gene37637:intron | 35.0% | |
| TGATGAACTTGAGGATGTTA+TGG | + | chr5.3:70714131-70714150 | MS.gene37637:CDS | 35.0% | |
| TTTATTCATCTCTGTGACAG+GGG | + | chr5.3:70714348-70714367 | MS.gene37637:intron | 35.0% | |
| ACTTGAGGATGTTATGGAAG+AGG | + | chr5.3:70714137-70714156 | MS.gene37637:CDS | 40.0% | |
| ATGTTGCAACTCTGACAGAT+TGG | + | chr5.3:70714505-70714524 | MS.gene37637:CDS | 40.0% | |
| CTTGAGGATGTTATGGAAGA+GGG | + | chr5.3:70714138-70714157 | MS.gene37637:CDS | 40.0% | |
| GATTTCACATTCGACGTTGT+TGG | - | chr5.3:70714394-70714413 | None:intergenic | 40.0% | |
| TGACAGATTGGATCAGGAAT+TGG | + | chr5.3:70714517-70714536 | MS.gene37637:CDS | 40.0% | |
| TTATTCATCTCTGTGACAGG+GGG | + | chr5.3:70714349-70714368 | MS.gene37637:intron | 40.0% | |
| ! | GGGAATATTGTGGACTATCA+TGG | + | chr5.3:70714162-70714181 | MS.gene37637:CDS | 40.0% |
| !!! | CTTTCAAGTTTTGCTTGAGG+AGG | + | chr5.3:70714413-70714432 | MS.gene37637:CDS | 40.0% |
| ACACAATCAAACCATCGCTC+GGG | - | chr5.3:70714201-70714220 | None:intergenic | 45.0% | |
| AGGATGTTATGGAAGAGGGA+GGG | + | chr5.3:70714142-70714161 | MS.gene37637:CDS | 45.0% | |
| CAACTCTGACAGATTGGATC+AGG | + | chr5.3:70714511-70714530 | MS.gene37637:CDS | 45.0% | |
| CACACAATCAAACCATCGCT+CGG | - | chr5.3:70714202-70714221 | None:intergenic | 45.0% | |
| GCTCAGACGGTCATACAAAA+TGG | - | chr5.3:70714247-70714266 | None:intergenic | 45.0% | |
| GGAGGCTAAAGAAAGTTACG+CGG | + | chr5.3:70714431-70714450 | MS.gene37637:CDS | 45.0% | |
| TGACATACCTTCTGCTCAGA+CGG | - | chr5.3:70714260-70714279 | None:intergenic | 45.0% | |
| CGAGCGATGGTTTGATTGTG+TGG | + | chr5.3:70714200-70714219 | MS.gene37637:CDS | 50.0% | |
| GAATTGGCCCATCCCAGTAT+AGG | + | chr5.3:70714533-70714552 | MS.gene37637:CDS | 50.0% | |
| GAGGATGTTATGGAAGAGGG+AGG | + | chr5.3:70714141-70714160 | MS.gene37637:CDS | 50.0% | |
| GGAAGAGGGAGGGAATATTG+TGG | + | chr5.3:70714152-70714171 | MS.gene37637:CDS | 50.0% | |
| GGCTAAAGAAAGTTACGCGG+AGG | + | chr5.3:70714434-70714453 | MS.gene37637:CDS | 50.0% | |
| GTGATTTCTTTCCCGAGCGA+TGG | + | chr5.3:70714187-70714206 | MS.gene37637:CDS | 50.0% | |
| TGTATGACCGTCTGAGCAGA+AGG | + | chr5.3:70714250-70714269 | MS.gene37637:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 70714126 | 70714554 | 70714126 | ID=MS.gene37637 |
| chr5.3 | mRNA | 70714126 | 70714554 | 70714126 | ID=MS.gene37637.t1;Parent=MS.gene37637 |
| chr5.3 | exon | 70714126 | 70714271 | 70714126 | ID=MS.gene37637.t1.exon1;Parent=MS.gene37637.t1 |
| chr5.3 | CDS | 70714126 | 70714271 | 70714126 | ID=cds.MS.gene37637.t1;Parent=MS.gene37637.t1 |
| chr5.3 | exon | 70714368 | 70714554 | 70714368 | ID=MS.gene37637.t1.exon2;Parent=MS.gene37637.t1 |
| chr5.3 | CDS | 70714368 | 70714554 | 70714368 | ID=cds.MS.gene37637.t1;Parent=MS.gene37637.t1 |
| Gene Sequence |
| Protein sequence |
