Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38199.t1 | XP_024638984.1 | 92.3 | 274 | 21 | 0 | 1 | 274 | 1 | 274 | 1.00E-144 | 522.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38199.t1 | Q9FMS6 | 59.9 | 277 | 104 | 4 | 3 | 272 | 1 | 277 | 1.2e-85 | 317.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38199.t1 | A0A396HXU6 | 92.3 | 274 | 21 | 0 | 1 | 274 | 1 | 274 | 7.4e-145 | 522.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene000609 | MS.gene38199 | PPI |
| MS.gene065707 | MS.gene38199 | PPI |
| MS.gene41101 | MS.gene38199 | PPI |
| MS.gene41097 | MS.gene38199 | PPI |
| MS.gene065708 | MS.gene38199 | PPI |
| MS.gene053225 | MS.gene38199 | PPI |
| MS.gene034815 | MS.gene38199 | PPI |
| MS.gene025629 | MS.gene38199 | PPI |
| MS.gene21541 | MS.gene38199 | PPI |
| MS.gene034250 | MS.gene38199 | PPI |
| MS.gene034816 | MS.gene38199 | PPI |
| MS.gene72977 | MS.gene38199 | PPI |
| MS.gene025628 | MS.gene38199 | PPI |
| MS.gene065709 | MS.gene38199 | PPI |
| MS.gene72978 | MS.gene38199 | PPI |
| MS.gene007154 | MS.gene38199 | PPI |
| MS.gene41100 | MS.gene38199 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38199.t1 | AT5G22250 | 59.928 | 277 | 104 | 4 | 3 | 272 | 1 | 277 | 2.67e-112 | 325 |
| MS.gene38199.t1 | AT3G44260 | 55.926 | 270 | 114 | 3 | 7 | 272 | 11 | 279 | 2.27e-104 | 305 |
| MS.gene38199.t1 | AT5G10960 | 46.667 | 270 | 140 | 4 | 4 | 272 | 1 | 267 | 1.15e-83 | 252 |
| MS.gene38199.t1 | AT2G32070 | 48.092 | 262 | 131 | 4 | 13 | 272 | 10 | 268 | 3.21e-82 | 248 |
| MS.gene38199.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 3.96e-79 | 241 |
| MS.gene38199.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 3.96e-79 | 241 |
| MS.gene38199.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 5.60e-79 | 241 |
| MS.gene38199.t1 | AT1G15920 | 45.788 | 273 | 136 | 6 | 7 | 271 | 8 | 276 | 1.30e-75 | 232 |
| MS.gene38199.t1 | AT1G15920 | 45.788 | 273 | 136 | 6 | 7 | 271 | 8 | 276 | 1.30e-75 | 232 |
| MS.gene38199.t1 | AT1G61470 | 36.885 | 244 | 133 | 7 | 14 | 251 | 1 | 229 | 2.71e-41 | 144 |
| MS.gene38199.t1 | AT1G06450 | 34.016 | 244 | 148 | 6 | 14 | 254 | 8 | 241 | 3.72e-36 | 132 |
| MS.gene38199.t1 | AT1G27820 | 34.000 | 250 | 140 | 9 | 11 | 251 | 2 | 235 | 7.86e-35 | 127 |
| MS.gene38199.t1 | AT3G44240 | 34.061 | 229 | 138 | 6 | 28 | 254 | 2 | 219 | 5.03e-33 | 121 |
| MS.gene38199.t1 | AT1G27890 | 33.471 | 242 | 140 | 8 | 17 | 251 | 3 | 230 | 1.11e-31 | 119 |
Find 58 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTACTCTAATGCACTTTA+TGG | 0.134494 | 5.3:+78764292 | MS.gene38199:CDS |
| TTCCACAGTGCGTACGATTT+TGG | 0.171860 | 5.3:+78764157 | MS.gene38199:CDS |
| CTCAATGGACACTGAATTTC+CGG | 0.199766 | 5.3:+78763790 | MS.gene38199:CDS |
| AATTGTATCCCCACACTTTA+CGG | 0.267621 | 5.3:-78763715 | None:intergenic |
| TTCTTTCGCAACACTGTTCT+AGG | 0.295965 | 5.3:+78764058 | MS.gene38199:CDS |
| TATTGACTTGCTCCGCCGTC+AGG | 0.317248 | 5.3:+78764027 | MS.gene38199:CDS |
| CAAGTAACTGTATTGCTCAT+TGG | 0.320243 | 5.3:-78763852 | None:intergenic |
| TCAGATCGGTCTTACCTTAT+CGG | 0.320555 | 5.3:+78763904 | MS.gene38199:CDS |
| TCCGTGACTTTAACGTCAAC+AGG | 0.331875 | 5.3:+78763984 | MS.gene38199:CDS |
| AAGTAACTGTATTGCTCATT+GGG | 0.348772 | 5.3:-78763851 | None:intergenic |
| ATGAGAACAGTCGCTACATC+TGG | 0.354062 | 5.3:+78763951 | MS.gene38199:CDS |
| AACTGTCCGATCCAGCTTGA+TGG | 0.397043 | 5.3:-78764369 | None:intergenic |
| CACACTTTACGGATCATGAT+TGG | 0.401574 | 5.3:-78763704 | None:intergenic |
| AAGCATGCTGGATCAATATT+CGG | 0.415547 | 5.3:+78764454 | MS.gene38199:CDS |
| TAAATACAATTTCATCTCAA+TGG | 0.417841 | 5.3:+78763775 | MS.gene38199:CDS |
| GCTACGCTGATGCGGTGGTC+TGG | 0.430872 | 5.3:+78764100 | MS.gene38199:CDS |
| CGAGTATTCTTCAAGTGGAT+CGG | 0.453450 | 5.3:+78764332 | MS.gene38199:CDS |
| AACTGTATTGCTCATTGGGT+TGG | 0.457601 | 5.3:-78763847 | None:intergenic |
| CTTACCTTATCGGATGCAAT+AGG | 0.458429 | 5.3:+78763914 | MS.gene38199:CDS |
| TTGGAGGCGACGACGGATTG+TGG | 0.464331 | 5.3:-78763828 | None:intergenic |
| TGTATTGCTCATTGGGTTGG+AGG | 0.469216 | 5.3:-78763844 | None:intergenic |
| ATTCAATATCATTCGTCAGT+TGG | 0.476456 | 5.3:+78763748 | MS.gene38199:CDS |
| CTTCAAGTGGATCGGGCCAT+TGG | 0.478735 | 5.3:+78764340 | MS.gene38199:CDS |
| TGGAGGCGACGACGGATTGT+GGG | 0.482460 | 5.3:-78763827 | None:intergenic |
| TCAATGGACACTGAATTTCC+GGG | 0.492924 | 5.3:+78763791 | MS.gene38199:CDS |
| GAGTATTCTTCAAGTGGATC+GGG | 0.498516 | 5.3:+78764333 | MS.gene38199:CDS |
| AATATCATTCGTCAGTTGGT+TGG | 0.513084 | 5.3:+78763752 | MS.gene38199:CDS |
| TGAGCAATACAGTTACTTGA+AGG | 0.514649 | 5.3:+78763856 | MS.gene38199:CDS |
| GTTACCTATTGCATCCGATA+AGG | 0.524193 | 5.3:-78763918 | None:intergenic |
| ATTGGTAAGTGCCATCAAGC+TGG | 0.527440 | 5.3:+78764358 | MS.gene38199:CDS |
| ATTGACTTGCTCCGCCGTCA+GGG | 0.527736 | 5.3:+78764028 | MS.gene38199:CDS |
| GACTGCAATGATAATGATGA+AGG | 0.560933 | 5.3:+78763664 | None:intergenic |
| GACGACGGATTGTGGGTGAA+TGG | 0.565974 | 5.3:-78763820 | None:intergenic |
| GTGGGTGAATGGACGATGCC+CGG | 0.568102 | 5.3:-78763809 | None:intergenic |
| CCCTGTTGACGTTAAAGTCA+CGG | 0.570625 | 5.3:-78763985 | None:intergenic |
| TACTCTAATGCACTTTATGG+CGG | 0.574864 | 5.3:+78764295 | MS.gene38199:CDS |
| CAATAGAGTCTTTGTTGTGG+AGG | 0.575807 | 5.3:-78764009 | None:intergenic |
| CATTGGGTTGGAGGCGACGA+CGG | 0.576634 | 5.3:-78763835 | None:intergenic |
| AATCATGATCCGTAAAGTGT+GGG | 0.578633 | 5.3:+78763706 | MS.gene38199:CDS |
| CATAAAGTGCATTAGAGTAA+CGG | 0.587105 | 5.3:-78764291 | None:intergenic |
| AGTTGCGAGTATTCTTCAAG+TGG | 0.590018 | 5.3:+78764327 | MS.gene38199:CDS |
| CAATCATGATCCGTAAAGTG+TGG | 0.590475 | 5.3:+78763705 | MS.gene38199:CDS |
| TGAGAACAGTCGCTACATCT+GGG | 0.592809 | 5.3:+78763952 | MS.gene38199:CDS |
| TAGCGACTGTTCTCATCATG+AGG | 0.601877 | 5.3:-78763944 | None:intergenic |
| TTTCAACAACTCAGTGACAT+GGG | 0.602278 | 5.3:+78764129 | MS.gene38199:CDS |
| TAAGTGCCATCAAGCTGGAT+CGG | 0.612952 | 5.3:+78764363 | MS.gene38199:CDS |
| AGCTTGATGGCACTTACCAA+TGG | 0.623590 | 5.3:-78764356 | None:intergenic |
| CCGTGACTTTAACGTCAACA+GGG | 0.626499 | 5.3:+78763985 | MS.gene38199:CDS |
| AGTCAATAGAGTCTTTGTTG+TGG | 0.637942 | 5.3:-78764012 | None:intergenic |
| GACTGTTCTCATCATGAGGG+AGG | 0.654467 | 5.3:-78763940 | None:intergenic |
| GATATTGAGAGCGTCAACGT+TGG | 0.662239 | 5.3:-78763879 | None:intergenic |
| ATCATGATCCGTAAAGTGTG+GGG | 0.662913 | 5.3:+78763707 | MS.gene38199:CDS |
| AGCGACTGTTCTCATCATGA+GGG | 0.665310 | 5.3:-78763943 | None:intergenic |
| ATCCAAAATCGTACGCACTG+TGG | 0.669380 | 5.3:-78764159 | None:intergenic |
| GCTCTCAATATCATTCAGAT+CGG | 0.674471 | 5.3:+78763890 | MS.gene38199:CDS |
| ATCTGGTGAAAATCTTAACG+CGG | 0.715080 | 5.3:+78764182 | MS.gene38199:CDS |
| GAAAGAAGTCGATACCCTGA+CGG | 0.733589 | 5.3:-78764042 | None:intergenic |
| AGAAGTCGATACCCTGACGG+CGG | 0.736815 | 5.3:-78764039 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATACAATTTCATCTCAA+TGG | + | chr5.3:78763775-78763794 | MS.gene38199:CDS | 20.0% |
| !!! | CATGTTTTGAGAGAATTATT+TGG | + | chr5.3:78764238-78764257 | MS.gene38199:CDS | 25.0% |
| AAGTAACTGTATTGCTCATT+GGG | - | chr5.3:78763854-78763873 | None:intergenic | 30.0% | |
| ATTCAATATCATTCGTCAGT+TGG | + | chr5.3:78763748-78763767 | MS.gene38199:CDS | 30.0% | |
| CATAAAGTGCATTAGAGTAA+CGG | - | chr5.3:78764294-78764313 | None:intergenic | 30.0% | |
| !!! | ATCATGATTGGTTTTGAACT+AGG | - | chr5.3:78763695-78763714 | None:intergenic | 30.0% |
| AAAAACTCTTTCAAGCTACG+AGG | - | chr5.3:78764217-78764236 | None:intergenic | 35.0% | |
| AAGCATGCTGGATCAATATT+CGG | + | chr5.3:78764454-78764473 | MS.gene38199:CDS | 35.0% | |
| AATATCATTCGTCAGTTGGT+TGG | + | chr5.3:78763752-78763771 | MS.gene38199:CDS | 35.0% | |
| AATCATGATCCGTAAAGTGT+GGG | + | chr5.3:78763706-78763725 | MS.gene38199:CDS | 35.0% | |
| AATTGTATCCCCACACTTTA+CGG | - | chr5.3:78763718-78763737 | None:intergenic | 35.0% | |
| AGTCAATAGAGTCTTTGTTG+TGG | - | chr5.3:78764015-78764034 | None:intergenic | 35.0% | |
| ATCTGGTGAAAATCTTAACG+CGG | + | chr5.3:78764182-78764201 | MS.gene38199:CDS | 35.0% | |
| CAAGTAACTGTATTGCTCAT+TGG | - | chr5.3:78763855-78763874 | None:intergenic | 35.0% | |
| CGTTACTCTAATGCACTTTA+TGG | + | chr5.3:78764292-78764311 | MS.gene38199:CDS | 35.0% | |
| GCTCTCAATATCATTCAGAT+CGG | + | chr5.3:78763890-78763909 | MS.gene38199:CDS | 35.0% | |
| TACTCTAATGCACTTTATGG+CGG | + | chr5.3:78764295-78764314 | MS.gene38199:CDS | 35.0% | |
| TGAGCAATACAGTTACTTGA+AGG | + | chr5.3:78763856-78763875 | MS.gene38199:CDS | 35.0% | |
| TTTCAACAACTCAGTGACAT+GGG | + | chr5.3:78764129-78764148 | MS.gene38199:CDS | 35.0% | |
| ! | AATGAAGTCAAAAAGCATGC+TGG | + | chr5.3:78764442-78764461 | MS.gene38199:CDS | 35.0% |
| ! | TTTTCAACAACTCAGTGACA+TGG | + | chr5.3:78764128-78764147 | MS.gene38199:CDS | 35.0% |
| AACTGTATTGCTCATTGGGT+TGG | - | chr5.3:78763850-78763869 | None:intergenic | 40.0% | |
| AGTTGCGAGTATTCTTCAAG+TGG | + | chr5.3:78764327-78764346 | MS.gene38199:CDS | 40.0% | |
| ATCATGATCCGTAAAGTGTG+GGG | + | chr5.3:78763707-78763726 | MS.gene38199:CDS | 40.0% | |
| CAATCATGATCCGTAAAGTG+TGG | + | chr5.3:78763705-78763724 | MS.gene38199:CDS | 40.0% | |
| CACACTTTACGGATCATGAT+TGG | - | chr5.3:78763707-78763726 | None:intergenic | 40.0% | |
| CGAGTATTCTTCAAGTGGAT+CGG | + | chr5.3:78764332-78764351 | MS.gene38199:CDS | 40.0% | |
| CTCAATGGACACTGAATTTC+CGG | + | chr5.3:78763790-78763809 | MS.gene38199:CDS | 40.0% | |
| CTTACCTTATCGGATGCAAT+AGG | + | chr5.3:78763914-78763933 | MS.gene38199:CDS | 40.0% | |
| GAGTATTCTTCAAGTGGATC+GGG | + | chr5.3:78764333-78764352 | MS.gene38199:CDS | 40.0% | |
| GTTACCTATTGCATCCGATA+AGG | - | chr5.3:78763921-78763940 | None:intergenic | 40.0% | |
| TCAATGGACACTGAATTTCC+GGG | + | chr5.3:78763791-78763810 | MS.gene38199:CDS | 40.0% | |
| TCAGATCGGTCTTACCTTAT+CGG | + | chr5.3:78763904-78763923 | MS.gene38199:CDS | 40.0% | |
| TTCTTTCGCAACACTGTTCT+AGG | + | chr5.3:78764058-78764077 | MS.gene38199:CDS | 40.0% | |
| ! | CAATAGAGTCTTTGTTGTGG+AGG | - | chr5.3:78764012-78764031 | None:intergenic | 40.0% |
| ! | TGCGTACGATTTTGGATATC+TGG | + | chr5.3:78764165-78764184 | MS.gene38199:CDS | 40.0% |
| ! | TTCATTTTGCTACGCTGATG+CGG | + | chr5.3:78764092-78764111 | MS.gene38199:CDS | 40.0% |
| AGCGACTGTTCTCATCATGA+GGG | - | chr5.3:78763946-78763965 | None:intergenic | 45.0% | |
| ATCCAAAATCGTACGCACTG+TGG | - | chr5.3:78764162-78764181 | None:intergenic | 45.0% | |
| ATGAGAACAGTCGCTACATC+TGG | + | chr5.3:78763951-78763970 | MS.gene38199:CDS | 45.0% | |
| ATTGGTAAGTGCCATCAAGC+TGG | + | chr5.3:78764358-78764377 | MS.gene38199:CDS | 45.0% | |
| CCCTGTTGACGTTAAAGTCA+CGG | - | chr5.3:78763988-78764007 | None:intergenic | 45.0% | |
| CCGTGACTTTAACGTCAACA+GGG | + | chr5.3:78763985-78764004 | MS.gene38199:CDS | 45.0% | |
| GAAAGAAGTCGATACCCTGA+CGG | - | chr5.3:78764045-78764064 | None:intergenic | 45.0% | |
| TAAGTGCCATCAAGCTGGAT+CGG | + | chr5.3:78764363-78764382 | MS.gene38199:CDS | 45.0% | |
| TAGCGACTGTTCTCATCATG+AGG | - | chr5.3:78763947-78763966 | None:intergenic | 45.0% | |
| TCCGTGACTTTAACGTCAAC+AGG | + | chr5.3:78763984-78764003 | MS.gene38199:CDS | 45.0% | |
| TGAGAACAGTCGCTACATCT+GGG | + | chr5.3:78763952-78763971 | MS.gene38199:CDS | 45.0% | |
| ! | AGCTTGATGGCACTTACCAA+TGG | - | chr5.3:78764359-78764378 | None:intergenic | 45.0% |
| ! | GTCGCAGACTTTTCACAAGA+TGG | + | chr5.3:78764399-78764418 | MS.gene38199:CDS | 45.0% |
| ! | TTCCACAGTGCGTACGATTT+TGG | + | chr5.3:78764157-78764176 | MS.gene38199:CDS | 45.0% |
| !! | GATATTGAGAGCGTCAACGT+TGG | - | chr5.3:78763882-78763901 | None:intergenic | 45.0% |
| !! | TGTATTGCTCATTGGGTTGG+AGG | - | chr5.3:78763847-78763866 | None:intergenic | 45.0% |
| AACTGTCCGATCCAGCTTGA+TGG | - | chr5.3:78764372-78764391 | None:intergenic | 50.0% | |
| GACTGTTCTCATCATGAGGG+AGG | - | chr5.3:78763943-78763962 | None:intergenic | 50.0% | |
| ! | ATTTTGCTACGCTGATGCGG+TGG | + | chr5.3:78764095-78764114 | MS.gene38199:CDS | 50.0% |
| AGAAGTCGATACCCTGACGG+CGG | - | chr5.3:78764042-78764061 | None:intergenic | 55.0% | |
| CTTCAAGTGGATCGGGCCAT+TGG | + | chr5.3:78764340-78764359 | MS.gene38199:CDS | 55.0% | |
| !! | ATTGACTTGCTCCGCCGTCA+GGG | + | chr5.3:78764028-78764047 | MS.gene38199:CDS | 55.0% |
| !! | GACGACGGATTGTGGGTGAA+TGG | - | chr5.3:78763823-78763842 | None:intergenic | 55.0% |
| !! | TATTGACTTGCTCCGCCGTC+AGG | + | chr5.3:78764027-78764046 | MS.gene38199:CDS | 55.0% |
| GTGGGTGAATGGACGATGCC+CGG | - | chr5.3:78763812-78763831 | None:intergenic | 60.0% | |
| !! | CATTGGGTTGGAGGCGACGA+CGG | - | chr5.3:78763838-78763857 | None:intergenic | 60.0% |
| !! | TGGAGGCGACGACGGATTGT+GGG | - | chr5.3:78763830-78763849 | None:intergenic | 60.0% |
| !! | TTGGAGGCGACGACGGATTG+TGG | - | chr5.3:78763831-78763850 | None:intergenic | 60.0% |
| GCTACGCTGATGCGGTGGTC+TGG | + | chr5.3:78764100-78764119 | MS.gene38199:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 78763671 | 78764495 | 78763671 | ID=MS.gene38199 |
| chr5.3 | mRNA | 78763671 | 78764495 | 78763671 | ID=MS.gene38199.t1;Parent=MS.gene38199 |
| chr5.3 | exon | 78763671 | 78764495 | 78763671 | ID=MS.gene38199.t1.exon1;Parent=MS.gene38199.t1 |
| chr5.3 | CDS | 78763671 | 78764495 | 78763671 | ID=cds.MS.gene38199.t1;Parent=MS.gene38199.t1 |
| Gene Sequence |
| Protein sequence |
