Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38201.t1 | XP_024638984.1 | 92.3 | 274 | 21 | 0 | 1 | 274 | 1 | 274 | 4.60E-145 | 523.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38201.t1 | Q9FMS6 | 59.9 | 277 | 104 | 4 | 3 | 272 | 1 | 277 | 4.2e-86 | 319.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38201.t1 | A0A396HXU6 | 92.3 | 274 | 21 | 0 | 1 | 274 | 1 | 274 | 3.3e-145 | 523.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene38201 | MS.gene007154 | PPI |
| MS.gene38201 | MS.gene72977 | PPI |
| MS.gene38201 | MS.gene41101 | PPI |
| MS.gene053225 | MS.gene38201 | PPI |
| MS.gene38201 | MS.gene065708 | PPI |
| MS.gene034815 | MS.gene38201 | PPI |
| MS.gene38201 | MS.gene065709 | PPI |
| MS.gene38201 | MS.gene065707 | PPI |
| MS.gene025629 | MS.gene38201 | PPI |
| MS.gene38201 | MS.gene025628 | PPI |
| MS.gene034250 | MS.gene38201 | PPI |
| MS.gene38201 | MS.gene034816 | PPI |
| MS.gene38201 | MS.gene72978 | PPI |
| MS.gene38201 | MS.gene41100 | PPI |
| MS.gene38201 | MS.gene41097 | PPI |
| MS.gene38201 | MS.gene000609 | PPI |
| MS.gene38201 | MS.gene21541 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38201.t1 | AT5G22250 | 59.928 | 277 | 104 | 4 | 3 | 272 | 1 | 277 | 2.58e-113 | 327 |
| MS.gene38201.t1 | AT3G44260 | 55.926 | 270 | 114 | 3 | 7 | 272 | 11 | 279 | 4.47e-105 | 306 |
| MS.gene38201.t1 | AT5G10960 | 46.667 | 270 | 140 | 4 | 4 | 272 | 1 | 267 | 1.76e-84 | 254 |
| MS.gene38201.t1 | AT2G32070 | 48.092 | 262 | 131 | 4 | 13 | 272 | 10 | 268 | 8.36e-82 | 247 |
| MS.gene38201.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 9.42e-80 | 242 |
| MS.gene38201.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 9.42e-80 | 242 |
| MS.gene38201.t1 | AT1G80780 | 46.743 | 261 | 135 | 4 | 13 | 272 | 10 | 267 | 1.32e-79 | 242 |
| MS.gene38201.t1 | AT1G15920 | 46.154 | 273 | 135 | 6 | 7 | 271 | 8 | 276 | 4.83e-76 | 233 |
| MS.gene38201.t1 | AT1G15920 | 46.154 | 273 | 135 | 6 | 7 | 271 | 8 | 276 | 4.83e-76 | 233 |
| MS.gene38201.t1 | AT1G61470 | 36.777 | 242 | 136 | 6 | 14 | 251 | 1 | 229 | 7.47e-41 | 142 |
| MS.gene38201.t1 | AT1G06450 | 34.426 | 244 | 147 | 6 | 14 | 254 | 8 | 241 | 1.86e-37 | 135 |
| MS.gene38201.t1 | AT3G44240 | 34.061 | 229 | 138 | 6 | 28 | 254 | 2 | 219 | 3.37e-34 | 124 |
| MS.gene38201.t1 | AT1G27820 | 34.000 | 250 | 140 | 9 | 11 | 251 | 2 | 235 | 5.79e-34 | 125 |
| MS.gene38201.t1 | AT1G27890 | 33.471 | 242 | 140 | 8 | 17 | 251 | 3 | 230 | 1.38e-31 | 119 |
Find 58 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTACTCTAATGCACTTTA+TGG | 0.122182 | 5.3:+78748857 | MS.gene38201:CDS |
| TTCCACAGTGCGTATGATTT+TGG | 0.151783 | 5.3:+78748722 | MS.gene38201:CDS |
| CTCAATGGACACTGAATTTC+CGG | 0.199766 | 5.3:+78748355 | MS.gene38201:CDS |
| AATTGTATCCCCACACTTTA+CGG | 0.267621 | 5.3:-78748280 | None:intergenic |
| TATTGACTTGCTCCGCCGTC+AGG | 0.317248 | 5.3:+78748592 | MS.gene38201:CDS |
| CAAGTAACTGTATTGCTCAT+TGG | 0.320243 | 5.3:-78748417 | None:intergenic |
| TCAGATCGGTCTTACCTTAT+CGG | 0.320555 | 5.3:+78748469 | MS.gene38201:CDS |
| TCCGTGACTTTAACGTCAAC+AGG | 0.331875 | 5.3:+78748549 | MS.gene38201:CDS |
| AAGTAACTGTATTGCTCATT+GGG | 0.348772 | 5.3:-78748416 | None:intergenic |
| ATGAGAACAGTCGCTACATC+TGG | 0.354062 | 5.3:+78748516 | MS.gene38201:CDS |
| TTCTTTCGCAACACTGTTCA+AGG | 0.356056 | 5.3:+78748623 | MS.gene38201:CDS |
| CACACTTTACGGATCATGAT+TGG | 0.401574 | 5.3:-78748269 | None:intergenic |
| TAAATACAATTTCATCTCAA+TGG | 0.417841 | 5.3:+78748340 | MS.gene38201:CDS |
| AACTGTCGGATCCAGCTTGA+TGG | 0.430459 | 5.3:-78748934 | None:intergenic |
| GCTACGCTGATGCGGTGGTC+TGG | 0.430872 | 5.3:+78748665 | MS.gene38201:CDS |
| AAGCACGCTGGATCAATATT+CGG | 0.452737 | 5.3:+78749019 | MS.gene38201:CDS |
| CGAGTATTCTTCAAGTGGAT+CGG | 0.453450 | 5.3:+78748897 | MS.gene38201:CDS |
| AACTGTATTGCTCATTGGGT+TGG | 0.457601 | 5.3:-78748412 | None:intergenic |
| CTTACCTTATCGGATGCAAT+AGG | 0.458429 | 5.3:+78748479 | MS.gene38201:CDS |
| TTGGAGGCGACGACGGATTG+TGG | 0.464331 | 5.3:-78748393 | None:intergenic |
| TGTATTGCTCATTGGGTTGG+AGG | 0.469216 | 5.3:-78748409 | None:intergenic |
| ATTCAATATCATTCGTCAGT+TGG | 0.476456 | 5.3:+78748313 | MS.gene38201:CDS |
| CTTCAAGTGGATCGGGCCAT+TGG | 0.478735 | 5.3:+78748905 | MS.gene38201:CDS |
| TGGAGGCGACGACGGATTGT+GGG | 0.482460 | 5.3:-78748392 | None:intergenic |
| TCAATGGACACTGAATTTCC+GGG | 0.492924 | 5.3:+78748356 | MS.gene38201:CDS |
| GAGTATTCTTCAAGTGGATC+GGG | 0.498516 | 5.3:+78748898 | MS.gene38201:CDS |
| AATATCATTCGTCAGTTGGT+TGG | 0.513084 | 5.3:+78748317 | MS.gene38201:CDS |
| TGAGCAATACAGTTACTTGA+AGG | 0.514649 | 5.3:+78748421 | MS.gene38201:CDS |
| GTTACCTATTGCATCCGATA+AGG | 0.524193 | 5.3:-78748483 | None:intergenic |
| ATTGGTAAGTGCCATCAAGC+TGG | 0.527440 | 5.3:+78748923 | MS.gene38201:CDS |
| ATTGACTTGCTCCGCCGTCA+GGG | 0.527736 | 5.3:+78748593 | MS.gene38201:CDS |
| GACTGCAATGATAATGATGA+AGG | 0.560933 | 5.3:+78748229 | None:intergenic |
| GACGACGGATTGTGGGTGAA+TGG | 0.565974 | 5.3:-78748385 | None:intergenic |
| GTGGGTGAATGGACGATGCC+CGG | 0.568102 | 5.3:-78748374 | None:intergenic |
| CCCTGTTGACGTTAAAGTCA+CGG | 0.570625 | 5.3:-78748550 | None:intergenic |
| CAATAGAGTCTTTGTTGTGG+AGG | 0.575807 | 5.3:-78748574 | None:intergenic |
| CATTGGGTTGGAGGCGACGA+CGG | 0.576634 | 5.3:-78748400 | None:intergenic |
| AATCATGATCCGTAAAGTGT+GGG | 0.578633 | 5.3:+78748271 | MS.gene38201:CDS |
| AGTTGCGAGTATTCTTCAAG+TGG | 0.590018 | 5.3:+78748892 | MS.gene38201:CDS |
| CAATCATGATCCGTAAAGTG+TGG | 0.590475 | 5.3:+78748270 | MS.gene38201:CDS |
| TGAGAACAGTCGCTACATCT+GGG | 0.592809 | 5.3:+78748517 | MS.gene38201:CDS |
| TAGCGACTGTTCTCATCATG+AGG | 0.601877 | 5.3:-78748509 | None:intergenic |
| TTTCAACAACTCAGTGACAT+GGG | 0.602278 | 5.3:+78748694 | MS.gene38201:CDS |
| TACTCTAATGCACTTTATGG+CGG | 0.605426 | 5.3:+78748860 | MS.gene38201:CDS |
| AGCTTGATGGCACTTACCAA+TGG | 0.623590 | 5.3:-78748921 | None:intergenic |
| CCGTGACTTTAACGTCAACA+GGG | 0.626499 | 5.3:+78748550 | MS.gene38201:CDS |
| AGTCAATAGAGTCTTTGTTG+TGG | 0.637942 | 5.3:-78748577 | None:intergenic |
| CATAAAGTGCATTAGAGTAG+CGG | 0.649775 | 5.3:-78748856 | None:intergenic |
| GACTGTTCTCATCATGAGGG+AGG | 0.654467 | 5.3:-78748505 | None:intergenic |
| GATATTGAGAGCGTCAACGT+TGG | 0.662239 | 5.3:-78748444 | None:intergenic |
| ATCATGATCCGTAAAGTGTG+GGG | 0.662913 | 5.3:+78748272 | MS.gene38201:CDS |
| AGCGACTGTTCTCATCATGA+GGG | 0.665310 | 5.3:-78748508 | None:intergenic |
| ATCCAAAATCATACGCACTG+TGG | 0.668840 | 5.3:-78748724 | None:intergenic |
| GCTCTCAATATCATTCAGAT+CGG | 0.674471 | 5.3:+78748455 | MS.gene38201:CDS |
| CTGCGACGTCAACAAACTGT+CGG | 0.689188 | 5.3:-78748948 | None:intergenic |
| ATCTGGTGAAAATCTTAACG+CGG | 0.715080 | 5.3:+78748747 | MS.gene38201:CDS |
| GAAAGAAGTCAATACCCTGA+CGG | 0.715946 | 5.3:-78748607 | None:intergenic |
| AGAAGTCAATACCCTGACGG+CGG | 0.736815 | 5.3:-78748604 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATACAATTTCATCTCAA+TGG | + | chr5.3:78748340-78748359 | MS.gene38201:CDS | 20.0% |
| !!! | CATGTTTTGAGAAAATTATT+TGG | + | chr5.3:78748803-78748822 | MS.gene38201:CDS | 20.0% |
| AAGTAACTGTATTGCTCATT+GGG | - | chr5.3:78748419-78748438 | None:intergenic | 30.0% | |
| ATTCAATATCATTCGTCAGT+TGG | + | chr5.3:78748313-78748332 | MS.gene38201:CDS | 30.0% | |
| !!! | ATCATGATTGGTTTTGAACT+AGG | - | chr5.3:78748260-78748279 | None:intergenic | 30.0% |
| AAAAACTCTTTCAAGCTACG+AGG | - | chr5.3:78748782-78748801 | None:intergenic | 35.0% | |
| AATATCATTCGTCAGTTGGT+TGG | + | chr5.3:78748317-78748336 | MS.gene38201:CDS | 35.0% | |
| AATCATGATCCGTAAAGTGT+GGG | + | chr5.3:78748271-78748290 | MS.gene38201:CDS | 35.0% | |
| AATTGTATCCCCACACTTTA+CGG | - | chr5.3:78748283-78748302 | None:intergenic | 35.0% | |
| AGTCAATAGAGTCTTTGTTG+TGG | - | chr5.3:78748580-78748599 | None:intergenic | 35.0% | |
| ATCTGGTGAAAATCTTAACG+CGG | + | chr5.3:78748747-78748766 | MS.gene38201:CDS | 35.0% | |
| CAAGTAACTGTATTGCTCAT+TGG | - | chr5.3:78748420-78748439 | None:intergenic | 35.0% | |
| CATAAAGTGCATTAGAGTAG+CGG | - | chr5.3:78748859-78748878 | None:intergenic | 35.0% | |
| GCTCTCAATATCATTCAGAT+CGG | + | chr5.3:78748455-78748474 | MS.gene38201:CDS | 35.0% | |
| TACTCTAATGCACTTTATGG+CGG | + | chr5.3:78748860-78748879 | MS.gene38201:CDS | 35.0% | |
| TGAGCAATACAGTTACTTGA+AGG | + | chr5.3:78748421-78748440 | MS.gene38201:CDS | 35.0% | |
| TTTCAACAACTCAGTGACAT+GGG | + | chr5.3:78748694-78748713 | MS.gene38201:CDS | 35.0% | |
| ! | TGCGTATGATTTTGGATATC+TGG | + | chr5.3:78748730-78748749 | MS.gene38201:CDS | 35.0% |
| ! | TTTTCAACAACTCAGTGACA+TGG | + | chr5.3:78748693-78748712 | MS.gene38201:CDS | 35.0% |
| AACTGTATTGCTCATTGGGT+TGG | - | chr5.3:78748415-78748434 | None:intergenic | 40.0% | |
| AAGCACGCTGGATCAATATT+CGG | + | chr5.3:78749019-78749038 | MS.gene38201:CDS | 40.0% | |
| AGTTGCGAGTATTCTTCAAG+TGG | + | chr5.3:78748892-78748911 | MS.gene38201:CDS | 40.0% | |
| ATCATGATCCGTAAAGTGTG+GGG | + | chr5.3:78748272-78748291 | MS.gene38201:CDS | 40.0% | |
| ATCCAAAATCATACGCACTG+TGG | - | chr5.3:78748727-78748746 | None:intergenic | 40.0% | |
| CAATCATGATCCGTAAAGTG+TGG | + | chr5.3:78748270-78748289 | MS.gene38201:CDS | 40.0% | |
| CACACTTTACGGATCATGAT+TGG | - | chr5.3:78748272-78748291 | None:intergenic | 40.0% | |
| CGAGTATTCTTCAAGTGGAT+CGG | + | chr5.3:78748897-78748916 | MS.gene38201:CDS | 40.0% | |
| CGCTACTCTAATGCACTTTA+TGG | + | chr5.3:78748857-78748876 | MS.gene38201:CDS | 40.0% | |
| CTCAATGGACACTGAATTTC+CGG | + | chr5.3:78748355-78748374 | MS.gene38201:CDS | 40.0% | |
| CTTACCTTATCGGATGCAAT+AGG | + | chr5.3:78748479-78748498 | MS.gene38201:CDS | 40.0% | |
| GAAAGAAGTCAATACCCTGA+CGG | - | chr5.3:78748610-78748629 | None:intergenic | 40.0% | |
| GAGTATTCTTCAAGTGGATC+GGG | + | chr5.3:78748898-78748917 | MS.gene38201:CDS | 40.0% | |
| GTTACCTATTGCATCCGATA+AGG | - | chr5.3:78748486-78748505 | None:intergenic | 40.0% | |
| TCAATGGACACTGAATTTCC+GGG | + | chr5.3:78748356-78748375 | MS.gene38201:CDS | 40.0% | |
| TCAGATCGGTCTTACCTTAT+CGG | + | chr5.3:78748469-78748488 | MS.gene38201:CDS | 40.0% | |
| TTCTTTCGCAACACTGTTCA+AGG | + | chr5.3:78748623-78748642 | MS.gene38201:CDS | 40.0% | |
| ! | AATGAAGTCAAAAAGCACGC+TGG | + | chr5.3:78749007-78749026 | MS.gene38201:CDS | 40.0% |
| ! | CAATAGAGTCTTTGTTGTGG+AGG | - | chr5.3:78748577-78748596 | None:intergenic | 40.0% |
| ! | TTCATTTTGCTACGCTGATG+CGG | + | chr5.3:78748657-78748676 | MS.gene38201:CDS | 40.0% |
| ! | TTCCACAGTGCGTATGATTT+TGG | + | chr5.3:78748722-78748741 | MS.gene38201:CDS | 40.0% |
| AGCGACTGTTCTCATCATGA+GGG | - | chr5.3:78748511-78748530 | None:intergenic | 45.0% | |
| ATGAGAACAGTCGCTACATC+TGG | + | chr5.3:78748516-78748535 | MS.gene38201:CDS | 45.0% | |
| ATTGGTAAGTGCCATCAAGC+TGG | + | chr5.3:78748923-78748942 | MS.gene38201:CDS | 45.0% | |
| CCCTGTTGACGTTAAAGTCA+CGG | - | chr5.3:78748553-78748572 | None:intergenic | 45.0% | |
| CCGTGACTTTAACGTCAACA+GGG | + | chr5.3:78748550-78748569 | MS.gene38201:CDS | 45.0% | |
| TAGCGACTGTTCTCATCATG+AGG | - | chr5.3:78748512-78748531 | None:intergenic | 45.0% | |
| TCCGTGACTTTAACGTCAAC+AGG | + | chr5.3:78748549-78748568 | MS.gene38201:CDS | 45.0% | |
| TGAGAACAGTCGCTACATCT+GGG | + | chr5.3:78748517-78748536 | MS.gene38201:CDS | 45.0% | |
| ! | AGCTTGATGGCACTTACCAA+TGG | - | chr5.3:78748924-78748943 | None:intergenic | 45.0% |
| ! | GTCGCAGACTTTTCACAAGA+TGG | + | chr5.3:78748964-78748983 | MS.gene38201:CDS | 45.0% |
| !! | GATATTGAGAGCGTCAACGT+TGG | - | chr5.3:78748447-78748466 | None:intergenic | 45.0% |
| !! | TGTATTGCTCATTGGGTTGG+AGG | - | chr5.3:78748412-78748431 | None:intergenic | 45.0% |
| AACTGTCGGATCCAGCTTGA+TGG | - | chr5.3:78748937-78748956 | None:intergenic | 50.0% | |
| AGAAGTCAATACCCTGACGG+CGG | - | chr5.3:78748607-78748626 | None:intergenic | 50.0% | |
| CTGCGACGTCAACAAACTGT+CGG | - | chr5.3:78748951-78748970 | None:intergenic | 50.0% | |
| GACTGTTCTCATCATGAGGG+AGG | - | chr5.3:78748508-78748527 | None:intergenic | 50.0% | |
| ! | ATTTTGCTACGCTGATGCGG+TGG | + | chr5.3:78748660-78748679 | MS.gene38201:CDS | 50.0% |
| CTTCAAGTGGATCGGGCCAT+TGG | + | chr5.3:78748905-78748924 | MS.gene38201:CDS | 55.0% | |
| !! | ATTGACTTGCTCCGCCGTCA+GGG | + | chr5.3:78748593-78748612 | MS.gene38201:CDS | 55.0% |
| !! | GACGACGGATTGTGGGTGAA+TGG | - | chr5.3:78748388-78748407 | None:intergenic | 55.0% |
| !! | TATTGACTTGCTCCGCCGTC+AGG | + | chr5.3:78748592-78748611 | MS.gene38201:CDS | 55.0% |
| GTGGGTGAATGGACGATGCC+CGG | - | chr5.3:78748377-78748396 | None:intergenic | 60.0% | |
| !! | CATTGGGTTGGAGGCGACGA+CGG | - | chr5.3:78748403-78748422 | None:intergenic | 60.0% |
| !! | TGGAGGCGACGACGGATTGT+GGG | - | chr5.3:78748395-78748414 | None:intergenic | 60.0% |
| !! | TTGGAGGCGACGACGGATTG+TGG | - | chr5.3:78748396-78748415 | None:intergenic | 60.0% |
| GCTACGCTGATGCGGTGGTC+TGG | + | chr5.3:78748665-78748684 | MS.gene38201:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 78748236 | 78749060 | 78748236 | ID=MS.gene38201 |
| chr5.3 | mRNA | 78748236 | 78749060 | 78748236 | ID=MS.gene38201.t1;Parent=MS.gene38201 |
| chr5.3 | exon | 78748236 | 78749060 | 78748236 | ID=MS.gene38201.t1.exon1;Parent=MS.gene38201.t1 |
| chr5.3 | CDS | 78748236 | 78749060 | 78748236 | ID=cds.MS.gene38201.t1;Parent=MS.gene38201.t1 |
| Gene Sequence |
| Protein sequence |
