AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41726


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41726.t1 MTR_7g085640 98.182 110 2 0 1 110 93 202 7.15e-66 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41726.t1 AT3G03920 79.817 109 22 0 2 110 94 202 5.23e-36 122
MS.gene41726.t1 AT5G18180 84.127 63 9 1 2 64 81 142 5.40e-30 106

Find 46 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGACGTGGTGGTGGTTTC+AGG 0.152779 7.2:+22158654 MS.gene41726:CDS
GGGAAAGTTGATGAAATATT+TGG 0.233909 7.2:+22157372 MS.gene41726:CDS
GGCCGTGGAGGTGGTGGTTT+TGG 0.280477 7.2:+22158557 MS.gene41726:CDS
GAGGACCTCTTCCACCCCTT+GGG 0.305063 7.2:-22158625 None:intergenic
CAGGGGAAGGGGCAGATTCT+AGG 0.306220 7.2:+22158673 MS.gene41726:CDS
GGAGGACCTCTTCCACCCCT+TGG 0.349525 7.2:-22158626 None:intergenic
GCTAGAGGCAAGAGCTTCCT+AGG 0.395733 7.2:-22157572 None:intergenic
CTGCAAAACATGACTCAGAT+TGG 0.398576 7.2:+22157351 None:intergenic
AGAGGTGGTGGCCGTGGAGG+TGG 0.402289 7.2:+22158581 MS.gene41726:CDS
GGTGGTGGTTTCAGGGGAAG+GGG 0.406506 7.2:+22158662 MS.gene41726:CDS
AGAGGTGGAGGCCGTGGAGG+TGG 0.421064 7.2:+22158548 MS.gene41726:CDS
TTTGGTAGAGGTGGTGGCCG+TGG 0.427705 7.2:+22158575 MS.gene41726:CDS
GTTGCTACTTCATATGCATC+TGG 0.429953 7.2:+22157530 MS.gene41726:CDS
GGTGGTAGAGGTGGAGGCCG+TGG 0.431868 7.2:+22158542 MS.gene41726:CDS
GTGGTGGTGGTTTCAGGGGA+AGG 0.432062 7.2:+22158660 MS.gene41726:CDS
TATTGCAGGGGACAAGCATC+AGG 0.437663 7.2:+22158521 MS.gene41726:intron
GGTGGAGGCCGTGGAGGTGG+TGG 0.442100 7.2:+22158551 MS.gene41726:CDS
CCACCACCACGTCCACCTCT+TGG 0.448869 7.2:-22158647 None:intergenic
GGTGGTGGCCGTGGAGGTGG+TGG 0.449672 7.2:+22158584 MS.gene41726:CDS
TGGTGGTGGTTTCAGGGGAA+GGG 0.471183 7.2:+22158661 MS.gene41726:CDS
TGCAAAACATGACTCAGATT+GGG 0.474986 7.2:+22157352 None:intergenic
TGGACGTGGTGGTGGTTTCA+GGG 0.482520 7.2:+22158655 MS.gene41726:CDS
CCAAGAGGTGGACGTGGTGG+TGG 0.483436 7.2:+22158647 MS.gene41726:CDS
AGGACCTCTTCCACCCCTTG+GGG 0.496542 7.2:-22158624 None:intergenic
GAAACTCACAGATTCATTGA+TGG 0.508035 7.2:-22157397 None:intergenic
CCACCACGTCCACCTCTTGG+AGG 0.512302 7.2:-22158644 None:intergenic
GGACAAGCATCAGGTGGTAG+AGG 0.524486 7.2:+22158530 MS.gene41726:CDS
TTTCTATTAAAATGATGGAA+GGG 0.525814 7.2:+22157504 MS.gene41726:CDS
AGGGGAAGGGGCAGATTCTA+GGG 0.527082 7.2:+22158674 MS.gene41726:CDS
GCATCAGGTGGTAGAGGTGG+AGG 0.530555 7.2:+22158536 MS.gene41726:CDS
CAAGCATCAGGTGGTAGAGG+TGG 0.537811 7.2:+22158533 MS.gene41726:CDS
GGACCTCTTCCACCCCTTGG+GGG 0.557362 7.2:-22158623 None:intergenic
GGTAGAGGTGGAGGCCGTGG+AGG 0.559108 7.2:+22158545 MS.gene41726:CDS
AAACTCACAGATTCATTGAT+GGG 0.576692 7.2:-22157396 None:intergenic
GGTAGAGGTGGTGGCCGTGG+AGG 0.585512 7.2:+22158578 MS.gene41726:CDS
GTGAGAACATACTTCGGCTG+AGG 0.591544 7.2:-22157605 None:intergenic
CACGAAAACCACCACCTCCA+CGG 0.595827 7.2:-22158592 None:intergenic
CCTCCAAGAGGTGGACGTGG+TGG 0.609010 7.2:+22158644 MS.gene41726:CDS
GGAAGAGGTCCTCCAAGAGG+TGG 0.617947 7.2:+22158635 MS.gene41726:CDS
GGTCCTCCAAGAGGTGGACG+TGG 0.619296 7.2:+22158641 MS.gene41726:CDS
CTAGAGGCAAGAGCTTCCTA+GGG 0.630481 7.2:-22157571 None:intergenic
TACCAAAACCACCACCTCCA+CGG 0.632501 7.2:-22158559 None:intergenic
GGACGTGGTGGTGGTTTCAG+GGG 0.633942 7.2:+22158656 MS.gene41726:CDS
TGAGGAAGAAATCTTGCTAG+AGG 0.638685 7.2:-22157587 None:intergenic
GGTGGAAGAGGTCCTCCAAG+AGG 0.663197 7.2:+22158632 MS.gene41726:CDS
TGCAGGGGACAAGCATCAGG+TGG 0.672433 7.2:+22158524 MS.gene41726:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAATATCAAAAACTAATTT+TGG - chr7.2:22158189-22158208 None:intergenic 15.0%
!!! CTAATATCAAAAACTAATTT+TGG - chr7.2:22158315-22158334 None:intergenic 15.0%
!!! TATTCATTTTTTCAGTAAAA+CGG - chr7.2:22157996-22158015 None:intergenic 15.0%
!!! TTTTTGATATTTGCTATATA+CGG + chr7.2:22158196-22158215 MS.gene41726:intron 15.0%
!! ACATCAGATAAAAATAAGAA+GGG + chr7.2:22158261-22158280 MS.gene41726:intron 20.0%
!! CATAAACAAGGAAATTAATT+AGG - chr7.2:22157710-22157729 None:intergenic 20.0%
!! TACATCAGATAAAAATAAGA+AGG + chr7.2:22158260-22158279 MS.gene41726:intron 20.0%
!!! ATTTTTTATTGGCTTTTGAA+AGG + chr7.2:22158458-22158477 MS.gene41726:intron 20.0%
!!! CTTATATGTTTTGTTGAAAT+GGG + chr7.2:22157443-22157462 MS.gene41726:intron 20.0%
!!! GTACTTTTCTATTAAAATGA+TGG + chr7.2:22157499-22157518 MS.gene41726:intron 20.0%
!!! TCTTATATGTTTTGTTGAAA+TGG + chr7.2:22157442-22157461 MS.gene41726:intron 20.0%
!!! TTTCTATTAAAATGATGGAA+GGG + chr7.2:22157504-22157523 MS.gene41726:CDS 20.0%
!!! TTTTCTATTAAAATGATGGA+AGG + chr7.2:22157503-22157522 MS.gene41726:CDS 20.0%
!!! TTTTTTATTGGCTTTTGAAA+GGG + chr7.2:22158459-22158478 MS.gene41726:intron 20.0%
! AATAAATTGTTGTATGCAGT+TGG + chr7.2:22158422-22158441 MS.gene41726:intron 25.0%
! AATTGACTGATTATTATTGC+AGG + chr7.2:22158507-22158526 MS.gene41726:intron 25.0%
! ATACTTGTTTGTTTGTTCAA+AGG + chr7.2:22158049-22158068 MS.gene41726:intron 25.0%
! ATCTGATGTAACATCAATTA+GGG - chr7.2:22158250-22158269 None:intergenic 25.0%
! ATTGACTGATTATTATTGCA+GGG + chr7.2:22158508-22158527 MS.gene41726:intron 25.0%
! CATACAAAACAATTAACACA+AGG - chr7.2:22158384-22158403 None:intergenic 25.0%
! CATCAGATAAAAATAAGAAG+GGG + chr7.2:22158262-22158281 MS.gene41726:intron 25.0%
! TATCTGATGTAACATCAATT+AGG - chr7.2:22158251-22158270 None:intergenic 25.0%
! TCAAAGGATTCTTATTGTTA+TGG + chr7.2:22158065-22158084 MS.gene41726:intron 25.0%
!! AATTTCCTTGTTTATGTTTG+TGG + chr7.2:22157714-22157733 MS.gene41726:intron 25.0%
!! ACTTAGACAGGTTAATTTTA+AGG - chr7.2:22157633-22157652 None:intergenic 25.0%
!! ATTGTACAGTTGATGTTAAA+TGG + chr7.2:22157653-22157672 MS.gene41726:intron 25.0%
!! TTGTACAGTTGATGTTAAAT+GGG + chr7.2:22157654-22157673 MS.gene41726:intron 25.0%
!!! CTTTGCTTGGAATTTTTTAT+TGG + chr7.2:22158447-22158466 MS.gene41726:intron 25.0%
AAACTCACAGATTCATTGAT+GGG - chr7.2:22157399-22157418 None:intergenic 30.0%
ACAATTAACACAAGGAAATG+TGG - chr7.2:22158376-22158395 None:intergenic 30.0%
CAATTAACACAAGGAAATGT+GGG - chr7.2:22158375-22158394 None:intergenic 30.0%
CATTACCACAAACATAAACA+AGG - chr7.2:22157722-22157741 None:intergenic 30.0%
TTGACTGATTATTATTGCAG+GGG + chr7.2:22158509-22158528 MS.gene41726:intron 30.0%
! CTATTTCTTGATTGCTATGT+TGG + chr7.2:22157768-22157787 MS.gene41726:intron 30.0%
!! GGGAAAGTTGATGAAATATT+TGG + chr7.2:22157372-22157391 MS.gene41726:CDS 30.0%
!! TGAAAGGGTATTACAATTGA+TGG + chr7.2:22158474-22158493 MS.gene41726:intron 30.0%
AAAATTAGACATCTGCAGCA+TGG - chr7.2:22158111-22158130 None:intergenic 35.0%
ATCTGTGAGTTTCTCTTTGT+TGG + chr7.2:22157407-22157426 MS.gene41726:intron 35.0%
CAACTGTACAATACTTAGAC+AGG - chr7.2:22157645-22157664 None:intergenic 35.0%
GAAACTCACAGATTCATTGA+TGG - chr7.2:22157400-22157419 None:intergenic 35.0%
GCAGATTACATCAAGACAAA+AGG - chr7.2:22158022-22158041 None:intergenic 35.0%
! ACAAGTTTTACATTGACCCT+AGG + chr7.2:22157555-22157574 MS.gene41726:CDS 35.0%
!! GTCTAATTTTGAGTGTGAGA+GGG + chr7.2:22158121-22158140 MS.gene41726:intron 35.0%
!! TGTCTAATTTTGAGTGTGAG+AGG + chr7.2:22158120-22158139 MS.gene41726:intron 35.0%
AAGGCAGTGAGAACATACTT+CGG - chr7.2:22157614-22157633 None:intergenic 40.0%
ACATCAATTAGGGACAGTAC+AGG - chr7.2:22158240-22158259 None:intergenic 40.0%
CAGTTCACACATTCTTCACA+TGG - chr7.2:22157858-22157877 None:intergenic 40.0%
TAACACAAGGAAATGTGGGA+CGG - chr7.2:22158371-22158390 None:intergenic 40.0%
TATGCAGTTGGATCTTTGCT+TGG + chr7.2:22158434-22158453 MS.gene41726:intron 40.0%
TGAGGAAGAAATCTTGCTAG+AGG - chr7.2:22157590-22157609 None:intergenic 40.0%
! GTTGCTACTTCATATGCATC+TGG + chr7.2:22157530-22157549 MS.gene41726:CDS 40.0%
GGTTACACTCTAGCATGTAG+AGG - chr7.2:22158344-22158363 None:intergenic 45.0%
!! TTGAGTGTGAGAGGGTGTTA+AGG + chr7.2:22158129-22158148 MS.gene41726:intron 45.0%
AAGGAAATGTGGGACGGTAC+AGG - chr7.2:22158365-22158384 None:intergenic 50.0%
CTAGAGGCAAGAGCTTCCTA+GGG - chr7.2:22157574-22157593 None:intergenic 50.0%
GTGAGAACATACTTCGGCTG+AGG - chr7.2:22157608-22157627 None:intergenic 50.0%
TACCAAAACCACCACCTCCA+CGG - chr7.2:22158562-22158581 None:intergenic 50.0%
TATTGCAGGGGACAAGCATC+AGG + chr7.2:22158521-22158540 MS.gene41726:intron 50.0%
!! AGAGGGTGTTAAGGAGAGGT+GGG + chr7.2:22158138-22158157 MS.gene41726:intron 50.0%
!! AGGTGGTGGTTTTCGTGGAA+GGG + chr7.2:22158598-22158617 MS.gene41726:CDS 50.0%
!! TGTGAGAGGGTGTTAAGGAG+AGG + chr7.2:22158134-22158153 MS.gene41726:intron 50.0%
CAAGCATCAGGTGGTAGAGG+TGG + chr7.2:22158533-22158552 MS.gene41726:CDS 55.0%
CACGAAAACCACCACCTCCA+CGG - chr7.2:22158595-22158614 None:intergenic 55.0%
GCTAGAGGCAAGAGCTTCCT+AGG - chr7.2:22157575-22157594 None:intergenic 55.0%
GGACAAGCATCAGGTGGTAG+AGG + chr7.2:22158530-22158549 MS.gene41726:CDS 55.0%
!! GAGAGGGTGTTAAGGAGAGG+TGG + chr7.2:22158137-22158156 MS.gene41726:intron 55.0%
!! GAGGTGGTGGTTTTCGTGGA+AGG + chr7.2:22158597-22158616 MS.gene41726:CDS 55.0%
!! GGTGGTGGTTTTCGTGGAAG+GGG + chr7.2:22158599-22158618 MS.gene41726:CDS 55.0%
!! TGGACGTGGTGGTGGTTTCA+GGG + chr7.2:22158655-22158674 MS.gene41726:CDS 55.0%
!! TGGTGGTGGTTTCAGGGGAA+GGG + chr7.2:22158661-22158680 MS.gene41726:CDS 55.0%
!!! GGAGGTGGTGGTTTTGGTAG+AGG + chr7.2:22158563-22158582 MS.gene41726:CDS 55.0%
!!! GGTGGTGGTTTTGGTAGAGG+TGG + chr7.2:22158566-22158585 MS.gene41726:CDS 55.0%
!!! GGTGGTTTTGGTAGAGGTGG+TGG + chr7.2:22158569-22158588 MS.gene41726:CDS 55.0%
AGGACCTCTTCCACCCCTTG+GGG - chr7.2:22158627-22158646 None:intergenic 60.0%
GAGGACCTCTTCCACCCCTT+GGG - chr7.2:22158628-22158647 None:intergenic 60.0%
GCATCAGGTGGTAGAGGTGG+AGG + chr7.2:22158536-22158555 MS.gene41726:CDS 60.0%
GGAAGAGGTCCTCCAAGAGG+TGG + chr7.2:22158635-22158654 MS.gene41726:CDS 60.0%
GGTGGAAGAGGTCCTCCAAG+AGG + chr7.2:22158632-22158651 MS.gene41726:CDS 60.0%
TGCAGGGGACAAGCATCAGG+TGG + chr7.2:22158524-22158543 MS.gene41726:intron 60.0%
! GGTGGTGGTTTCAGGGGAAG+GGG + chr7.2:22158662-22158681 MS.gene41726:CDS 60.0%
! TTTGGTAGAGGTGGTGGCCG+TGG + chr7.2:22158575-22158594 MS.gene41726:CDS 60.0%
!! CGTGGAGGTGGTGGTTTTCG+TGG + chr7.2:22158593-22158612 MS.gene41726:CDS 60.0%
!! GGACGTGGTGGTGGTTTCAG+GGG + chr7.2:22158656-22158675 MS.gene41726:CDS 60.0%
!! GTGGACGTGGTGGTGGTTTC+AGG + chr7.2:22158654-22158673 MS.gene41726:CDS 60.0%
!! GTGGTGGTGGTTTCAGGGGA+AGG + chr7.2:22158660-22158679 MS.gene41726:CDS 60.0%
CCAAGAGGTGGACGTGGTGG+TGG + chr7.2:22158647-22158666 MS.gene41726:CDS 65.0%
CCACCACCACGTCCACCTCT+TGG - chr7.2:22158650-22158669 None:intergenic 65.0%
CCACCACGTCCACCTCTTGG+AGG - chr7.2:22158647-22158666 None:intergenic 65.0%
CCTCCAAGAGGTGGACGTGG+TGG + chr7.2:22158644-22158663 MS.gene41726:CDS 65.0%
GGACCTCTTCCACCCCTTGG+GGG - chr7.2:22158626-22158645 None:intergenic 65.0%
GGAGGACCTCTTCCACCCCT+TGG - chr7.2:22158629-22158648 None:intergenic 65.0%
GGTCCTCCAAGAGGTGGACG+TGG + chr7.2:22158641-22158660 MS.gene41726:CDS 65.0%
TCGTGGAAGGGGTCCCCCAA+GGG + chr7.2:22158610-22158629 MS.gene41726:CDS 65.0%
TTCGTGGAAGGGGTCCCCCA+AGG + chr7.2:22158609-22158628 MS.gene41726:CDS 65.0%
!! GGCCGTGGAGGTGGTGGTTT+TGG + chr7.2:22158557-22158576 MS.gene41726:CDS 65.0%
AGAGGTGGAGGCCGTGGAGG+TGG + chr7.2:22158548-22158567 MS.gene41726:CDS 70.0%
CGTGGAAGGGGTCCCCCAAG+GGG + chr7.2:22158611-22158630 MS.gene41726:CDS 70.0%
GGTAGAGGTGGAGGCCGTGG+AGG + chr7.2:22158545-22158564 MS.gene41726:CDS 70.0%
GGTCCCCCAAGGGGTGGAAG+AGG + chr7.2:22158620-22158639 MS.gene41726:CDS 70.0%
GGTGGTAGAGGTGGAGGCCG+TGG + chr7.2:22158542-22158561 MS.gene41726:CDS 70.0%
! AGAGGTGGTGGCCGTGGAGG+TGG + chr7.2:22158581-22158600 MS.gene41726:CDS 70.0%
! GGTAGAGGTGGTGGCCGTGG+AGG + chr7.2:22158578-22158597 MS.gene41726:CDS 70.0%
GGAAGGGGTCCCCCAAGGGG+TGG + chr7.2:22158614-22158633 MS.gene41726:CDS 75.0%
GGTGGAGGCCGTGGAGGTGG+TGG + chr7.2:22158551-22158570 MS.gene41726:CDS 75.0%
! GGTGGTGGCCGTGGAGGTGG+TGG + chr7.2:22158584-22158603 MS.gene41726:CDS 75.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 22157360 22158694 22157360 ID=MS.gene41726
chr7.2 mRNA 22157360 22158694 22157360 ID=MS.gene41726.t1;Parent=MS.gene41726
chr7.2 exon 22157360 22157410 22157360 ID=MS.gene41726.t1.exon1;Parent=MS.gene41726.t1
chr7.2 CDS 22157360 22157410 22157360 ID=cds.MS.gene41726.t1;Parent=MS.gene41726.t1
chr7.2 exon 22157500 22157615 22157500 ID=MS.gene41726.t1.exon2;Parent=MS.gene41726.t1
chr7.2 CDS 22157500 22157615 22157500 ID=cds.MS.gene41726.t1;Parent=MS.gene41726.t1
chr7.2 exon 22158529 22158694 22158529 ID=MS.gene41726.t1.exon3;Parent=MS.gene41726.t1
chr7.2 CDS 22158529 22158694 22158529 ID=cds.MS.gene41726.t1;Parent=MS.gene41726.t1
Gene Sequence

>MS.gene41726

ATGACTCAGATTGGGAAAGTTGATGAAATATTTGGTCCCATCAATGAATCTGTGAGTTTCTCTTTGTTGGTTGATTTTATCTTCTTATATGTTTTGTTGAAATGGGTTTTTATGTAATTTTTGATTTGTTTGAATTGCAGTACTTTTCTATTAAAATGATGGAAGGGATTGTTGCTACTTCATATGCATCTGGTGACAAGTTTTACATTGACCCTAGGAAGCTCTTGCCTCTAGCAAGATTTCTTCCTCAGCCGAAGTATGTTCTCACTGCCTTAAAATTAACCTGTCTAAGTATTGTACAGTTGATGTTAAATGGGTATTGAAAAGTAGTTTGACATAAAGATTTTCCTAATTAATTTCCTTGTTTATGTTTGTGGTAATGTTTGTTTTGTATATCTAACACTTGAACTATTTCTTGATTGCTATGTTGGTGTATTGTGTTGCATATCATAGCATGATGCTATTCATGCATCATTACTGTTATGTTTGTTATAACCATGTGAAGAATGTGTGAACTGCTTTATATTTCACATGTTGTTACTGTTATGTTTGCAGTTTGTTAAAAGTTGTGCTTATTTCGTTGTGAGCATGCAGTGCTTCTATTATTAACTAAATTAATGATTGCTTGTGAAACCGTTTTACTGAAAAAATGAATATGACCTTTTGTCTTGATGTAATCTGCTATGATTATACTTGTTTGTTTGTTCAAAGGATTCTTATTGTTATGGTTGTTGAGACATGCATTAGACCATGCTGCAGATGTCTAATTTTGAGTGTGAGAGGGTGTTAAGGAGAGGTGGGTGATGATTTACGTAGAATGATGAAGCCAAAATTAGTTTTTGATATTTGCTATATACGGATACATGCTAGAATGTAACCTGTACTGTCCCTAATTGATGTTACATCAGATAAAAATAAGAAGGGGCAATGAATTACTGAGATATGATGAAAGCCAAAATTAGTTTTTGATATTAGCTACATCCTCTACATGCTAGAGTGTAACCTGTACCGTCCCACATTTCCTTGTGTTAATTGTTTTGTATGCAGTTCTGCATAAAATAAAATAAATTGTTGTATGCAGTTGGATCTTTGCTTGGAATTTTTTATTGGCTTTTGAAAGGGTATTACAATTGATGGAAGTTAACACAATTGACTGATTATTATTGCAGGGGACAAGCATCAGGTGGTAGAGGTGGAGGCCGTGGAGGTGGTGGTTTTGGTAGAGGTGGTGGCCGTGGAGGTGGTGGTTTTCGTGGAAGGGGTCCCCCAAGGGGTGGAAGAGGTCCTCCAAGAGGTGGACGTGGTGGTGGTTTCAGGGGAAGGGGCAGATTCTAG

Protein sequence

>MS.gene41726.t1

MTQIGKVDEIFGPINESYFSIKMMEGIVATSYASGDKFYIDPRKLLPLARFLPQPKGQASGGRGGGRGGGGFGRGGGRGGGGFRGRGPPRGGRGPPRGGRGGGFRGRGRF