Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43643.t1 | XP_003615419.1 | 92.1 | 151 | 11 | 1 | 1 | 151 | 1 | 150 | 1.40E-71 | 278.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43643.t1 | G7KD75 | 92.1 | 151 | 11 | 1 | 1 | 151 | 1 | 150 | 1.0e-71 | 278.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene43643 | 0.824927 | 6.19E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene43643 | 0.820283 | 7.44E-53 | -1.69E-46 |
| MS.gene050945 | MS.gene43643 | 0.825424 | 4.72E-54 | -1.69E-46 |
| MS.gene050955 | MS.gene43643 | 0.803745 | 3.01E-49 | -1.69E-46 |
| MS.gene051003 | MS.gene43643 | 0.806818 | 6.84E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene43643 | 0.805143 | 1.54E-49 | -1.69E-46 |
| MS.gene051275 | MS.gene43643 | 0.805101 | 1.57E-49 | -1.69E-46 |
| MS.gene051343 | MS.gene43643 | 0.82834 | 9.50E-55 | -1.69E-46 |
| MS.gene051902 | MS.gene43643 | 0.808961 | 2.39E-50 | -1.69E-46 |
| MS.gene051975 | MS.gene43643 | 0.800069 | 1.71E-48 | -1.69E-46 |
| MS.gene053058 | MS.gene43643 | 0.819767 | 9.77E-53 | -1.69E-46 |
| MS.gene053564 | MS.gene43643 | 0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene43643 | 0.817393 | 3.38E-52 | -1.69E-46 |
| MS.gene054403 | MS.gene43643 | 0.823406 | 1.41E-53 | -1.69E-46 |
| MS.gene059146 | MS.gene43643 | 0.820453 | 6.80E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43643.t1 | MTR_5g067740 | 92.053 | 151 | 11 | 1 | 1 | 151 | 1 | 150 | 1.37e-99 | 283 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene43643.t1 | AT5G05220 | 56.250 | 64 | 26 | 1 | 90 | 151 | 71 | 134 | 7.22e-18 | 76.3 |
| MS.gene43643.t1 | AT5G05220 | 61.364 | 44 | 17 | 0 | 107 | 150 | 136 | 179 | 1.24e-12 | 62.4 |
Find 33 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTGGGATGTGCTAGTT+GGG | 0.317379 | 5.1:-52526125 | MS.gene43643:CDS |
| TTTGGAAAAGGAAGCAATAA+TGG | 0.320210 | 5.1:-52525972 | MS.gene43643:CDS |
| GGAATTTCTCATAAACAAAA+AGG | 0.325228 | 5.1:-52526160 | MS.gene43643:CDS |
| TGGAGAAAGGGAAGGGGTTG+TGG | 0.334523 | 5.1:-52526008 | MS.gene43643:CDS |
| GGTTTCAATGTCACATTACT+AGG | 0.361956 | 5.1:+52526199 | None:intergenic |
| TGAAGTATTTCAAGCAGTTA+AGG | 0.368214 | 5.1:-52525885 | MS.gene43643:CDS |
| TGAGATTGATGATGAAGAAA+AGG | 0.384552 | 5.1:-52526065 | MS.gene43643:CDS |
| GATGAAGAAAAGGGTAAAAG+TGG | 0.435549 | 5.1:-52526055 | MS.gene43643:CDS |
| ATTCCCACCTTTGTCCTCAA+TGG | 0.437329 | 5.1:+52526178 | None:intergenic |
| GGGATGTGCTAGTTGGGGAT+TGG | 0.446491 | 5.1:-52526119 | MS.gene43643:CDS |
| GAGATTGATGATGAAGAAAA+GGG | 0.457481 | 5.1:-52526064 | MS.gene43643:CDS |
| TAGAGAAAAGTGTGGAGAAA+GGG | 0.459998 | 5.1:-52526020 | MS.gene43643:CDS |
| TTGTTGTGGGATGTGCTAGT+TGG | 0.465218 | 5.1:-52526126 | MS.gene43643:CDS |
| ATAGAGAAAAGTGTGGAGAA+AGG | 0.467324 | 5.1:-52526021 | MS.gene43643:CDS |
| GCCCTTCGATTGTAATCATT+AGG | 0.469511 | 5.1:+52525929 | None:intergenic |
| AGGTAAAAGGGAATTTGTTG+TGG | 0.499813 | 5.1:-52526140 | MS.gene43643:CDS |
| TTGGAAAAGGAAGCAATAAT+GGG | 0.513066 | 5.1:-52525971 | MS.gene43643:CDS |
| AAAAGTGTGGAGAAAGGGAA+GGG | 0.513638 | 5.1:-52526015 | MS.gene43643:CDS |
| GGTAAAAGGGAATTTGTTGT+GGG | 0.517108 | 5.1:-52526139 | MS.gene43643:CDS |
| AGCCTAATGATTACAATCGA+AGG | 0.534075 | 5.1:-52525931 | MS.gene43643:CDS |
| GCAATAATGGGTGATGATGA+AGG | 0.538475 | 5.1:-52525959 | MS.gene43643:CDS |
| ATCATGAACATTAGCAACAA+AGG | 0.562063 | 5.1:+52526285 | None:intergenic |
| GAAAAGTGTGGAGAAAGGGA+AGG | 0.573580 | 5.1:-52526016 | MS.gene43643:CDS |
| AAAGTGTGGAGAAAGGGAAG+GGG | 0.577573 | 5.1:-52526014 | MS.gene43643:CDS |
| GAAACCATTGAGGACAAAGG+TGG | 0.582861 | 5.1:-52526182 | MS.gene43643:CDS |
| GAGTTAGAACAAATCAAAGA+AGG | 0.587741 | 5.1:+52526250 | None:intergenic |
| ACAAGGTATAGAGAAAAGTG+TGG | 0.590729 | 5.1:-52526028 | MS.gene43643:CDS |
| GCCTAATGATTACAATCGAA+GGG | 0.594969 | 5.1:-52525930 | MS.gene43643:CDS |
| GTTGTGGGATGTGCTAGTTG+GGG | 0.607040 | 5.1:-52526124 | MS.gene43643:CDS |
| AGGGTAAAAGTGGAATAACA+AGG | 0.635782 | 5.1:-52526045 | MS.gene43643:CDS |
| AAACCATTGAGGACAAAGGT+GGG | 0.637541 | 5.1:-52526181 | MS.gene43643:CDS |
| ATGTGACATTGAAACCATTG+AGG | 0.645312 | 5.1:-52526192 | MS.gene43643:CDS |
| ATTGAAACCATTGAGGACAA+AGG | 0.674535 | 5.1:-52526185 | MS.gene43643:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATAAACAAAAAGGTAAAA+GGG | - | chr5.1:52526011-52526030 | MS.gene43643:CDS | 20.0% |
| ! | CTCATAAACAAAAAGGTAAA+AGG | - | chr5.1:52526010-52526029 | MS.gene43643:CDS | 25.0% |
| ! | GGAATTTCTCATAAACAAAA+AGG | - | chr5.1:52526003-52526022 | MS.gene43643:CDS | 25.0% |
| ! | TACTAGGAAAAACATTACTT+TGG | + | chr5.1:52525951-52525970 | None:intergenic | 25.0% |
| ATCATGAACATTAGCAACAA+AGG | + | chr5.1:52525881-52525900 | None:intergenic | 30.0% | |
| GAGTTAGAACAAATCAAAGA+AGG | + | chr5.1:52525916-52525935 | None:intergenic | 30.0% | |
| TTGGAAAAGGAAGCAATAAT+GGG | - | chr5.1:52526192-52526211 | MS.gene43643:CDS | 30.0% | |
| TTTGGAAAAGGAAGCAATAA+TGG | - | chr5.1:52526191-52526210 | MS.gene43643:CDS | 30.0% | |
| ! | GGAAAAACATTACTTTGGAA+TGG | + | chr5.1:52525946-52525965 | None:intergenic | 30.0% |
| ! | TGAAGTATTTCAAGCAGTTA+AGG | - | chr5.1:52526278-52526297 | MS.gene43643:CDS | 30.0% |
| !! | GAGATTGATGATGAAGAAAA+GGG | - | chr5.1:52526099-52526118 | MS.gene43643:CDS | 30.0% |
| !! | TGAGATTGATGATGAAGAAA+AGG | - | chr5.1:52526098-52526117 | MS.gene43643:CDS | 30.0% |
| ACAAGGTATAGAGAAAAGTG+TGG | - | chr5.1:52526135-52526154 | MS.gene43643:CDS | 35.0% | |
| AGCCTAATGATTACAATCGA+AGG | - | chr5.1:52526232-52526251 | MS.gene43643:CDS | 35.0% | |
| AGGGTAAAAGTGGAATAACA+AGG | - | chr5.1:52526118-52526137 | MS.gene43643:CDS | 35.0% | |
| ATGTGACATTGAAACCATTG+AGG | - | chr5.1:52525971-52525990 | MS.gene43643:CDS | 35.0% | |
| ATTGAAACCATTGAGGACAA+AGG | - | chr5.1:52525978-52525997 | MS.gene43643:CDS | 35.0% | |
| GATGAAGAAAAGGGTAAAAG+TGG | - | chr5.1:52526108-52526127 | MS.gene43643:CDS | 35.0% | |
| GCCTAATGATTACAATCGAA+GGG | - | chr5.1:52526233-52526252 | MS.gene43643:CDS | 35.0% | |
| GGTTTCAATGTCACATTACT+AGG | + | chr5.1:52525967-52525986 | None:intergenic | 35.0% | |
| ! | ATAGAGAAAAGTGTGGAGAA+AGG | - | chr5.1:52526142-52526161 | MS.gene43643:CDS | 35.0% |
| ! | TAGAGAAAAGTGTGGAGAAA+GGG | - | chr5.1:52526143-52526162 | MS.gene43643:CDS | 35.0% |
| !! | AGGTAAAAGGGAATTTGTTG+TGG | - | chr5.1:52526023-52526042 | MS.gene43643:CDS | 35.0% |
| !! | GGTAAAAGGGAATTTGTTGT+GGG | - | chr5.1:52526024-52526043 | MS.gene43643:CDS | 35.0% |
| !!! | GTTTTTGGAGTGTTTGGAAA+AGG | - | chr5.1:52526179-52526198 | MS.gene43643:CDS | 35.0% |
| AAACCATTGAGGACAAAGGT+GGG | - | chr5.1:52525982-52526001 | MS.gene43643:CDS | 40.0% | |
| GCCCTTCGATTGTAATCATT+AGG | + | chr5.1:52526237-52526256 | None:intergenic | 40.0% | |
| ! | AAAAGTGTGGAGAAAGGGAA+GGG | - | chr5.1:52526148-52526167 | MS.gene43643:CDS | 40.0% |
| !! | GCAATAATGGGTGATGATGA+AGG | - | chr5.1:52526204-52526223 | MS.gene43643:CDS | 40.0% |
| !!! | TGTGGAGTTTTTGGAGTGTT+TGG | - | chr5.1:52526173-52526192 | MS.gene43643:CDS | 40.0% |
| AAAGTGTGGAGAAAGGGAAG+GGG | - | chr5.1:52526149-52526168 | MS.gene43643:CDS | 45.0% | |
| ATTCCCACCTTTGTCCTCAA+TGG | + | chr5.1:52525988-52526007 | None:intergenic | 45.0% | |
| GAAACCATTGAGGACAAAGG+TGG | - | chr5.1:52525981-52526000 | MS.gene43643:CDS | 45.0% | |
| TGTTGTGGGATGTGCTAGTT+GGG | - | chr5.1:52526038-52526057 | MS.gene43643:CDS | 45.0% | |
| TTGTTGTGGGATGTGCTAGT+TGG | - | chr5.1:52526037-52526056 | MS.gene43643:CDS | 45.0% | |
| ! | GAAAAGTGTGGAGAAAGGGA+AGG | - | chr5.1:52526147-52526166 | MS.gene43643:CDS | 45.0% |
| GTTGTGGGATGTGCTAGTTG+GGG | - | chr5.1:52526039-52526058 | MS.gene43643:CDS | 50.0% | |
| ! | GGAAGGGGTTGTGGAGTTTT+TGG | - | chr5.1:52526164-52526183 | MS.gene43643:CDS | 50.0% |
| GGGATGTGCTAGTTGGGGAT+TGG | - | chr5.1:52526044-52526063 | MS.gene43643:CDS | 55.0% | |
| TGGAGAAAGGGAAGGGGTTG+TGG | - | chr5.1:52526155-52526174 | MS.gene43643:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 52525865 | 52526320 | 52525865 | ID=MS.gene43643 |
| chr5.1 | mRNA | 52525865 | 52526320 | 52525865 | ID=MS.gene43643.t1;Parent=MS.gene43643 |
| chr5.1 | exon | 52525865 | 52526320 | 52525865 | ID=MS.gene43643.t1.exon1;Parent=MS.gene43643.t1 |
| chr5.1 | CDS | 52525865 | 52526320 | 52525865 | ID=cds.MS.gene43643.t1;Parent=MS.gene43643.t1 |
| Gene Sequence |
| Protein sequence |
