AlfalfaGEDB | MS.gene43987

M. sativa cultivar XinJiangDaYe / MS.gene43987

KEGG: K11275
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0006325 GO:0006334 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0032991 GO:0032993 GO:0034622 GO:0034728 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044815 GO:0065003 GO:0065004 GO:0071824 GO:0071840 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00538.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43987.t1	XP_013468967.1	95.8	96	2	1	1	96	1	94	9.70E-38	166.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43987.t1	P08283	68.9	74	23	0	22	95	59	132	4.6e-20	99.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43987.t1	A0A072VLX5	95.8	96	2	1	1	96	1	94	7.0e-38	166.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05124	MS.gene43987	0.803884	2.82E-49	-1.69E-46
MS.gene051914	MS.gene43987	0.814	1.92E-51	-1.69E-46
MS.gene052308	MS.gene43987	0.813216	2.86E-51	-1.69E-46
MS.gene053173	MS.gene43987	0.830361	3.07E-55	-1.69E-46
MS.gene054018	MS.gene43987	0.806488	8.03E-50	-1.69E-46
MS.gene054551	MS.gene43987	0.823997	1.02E-53	-1.69E-46
MS.gene054593	MS.gene43987	0.805565	1.26E-49	-1.69E-46
MS.gene054633	MS.gene43987	0.850437	1.70E-60	-1.69E-46
MS.gene055578	MS.gene43987	0.814652	1.38E-51	-1.69E-46
MS.gene05640	MS.gene43987	0.847219	1.33E-59	-1.69E-46
MS.gene057458	MS.gene43987	0.801571	8.46E-49	-1.69E-46
MS.gene058128	MS.gene43987	0.813926	2.00E-51	-1.69E-46
MS.gene058853	MS.gene43987	0.821962	3.05E-53	-1.69E-46
MS.gene06093	MS.gene43987	0.812763	3.60E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43987.t1	MTR_1g080700	88.000	100	10	1	1	100	1	98	2.27e-51	163
MS.gene43987.t1	MTR_1g070390	69.880	83	25	0	12	94	50	132	7.95e-27	102
MS.gene43987.t1	MTR_1g070435	69.880	83	25	0	12	94	50	132	1.95e-26	101
MS.gene43987.t1	MTR_7g096280	61.644	73	28	0	19	91	56	128	1.19e-25	100
MS.gene43987.t1	MTR_1g070440	56.296	135	27	4	24	150	9	119	2.75e-25	97.8
MS.gene43987.t1	MTR_5g021730	46.316	95	39	3	1	94	22	105	9.55e-18	76.6
MS.gene43987.t1	MTR_8g077160	69.048	42	13	0	12	53	49	90	2.48e-13	63.2
MS.gene43987.t1	MTR_3g065570	42.857	70	40	0	20	89	8	77	7.73e-13	63.9
MS.gene43987.t1	MTR_6g079520	44.444	72	37	1	23	91	40	111	1.80e-12	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43987.t1	AT2G30620	57.025	121	25	2	24	142	61	156	6.60e-33	117
MS.gene43987.t1	AT2G30620	57.143	119	24	2	24	140	61	154	7.96e-32	116
MS.gene43987.t1	AT1G06760	77.941	68	15	0	24	91	61	128	1.28e-23	94.7
MS.gene43987.t1	AT2G18050	44.094	127	62	3	14	131	8	134	6.03e-21	85.1
MS.gene43987.t1	AT2G18050	39.024	123	70	3	31	148	1	123	9.51e-17	73.6

Find 50 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGGCTTCGCCGCTGCTTT+TGG	0.203938	1.3:+57100609	None:intergenic
TTTGGCTTTGCGGCAGCTTT+CGG	0.250457	1.3:+57100627	None:intergenic
CCCTTAACACTCTTCGCTTT+CGG	0.311339	1.3:+57100573	None:intergenic
CTGAAGAAATCTGTGGCTTC+TGG	0.328322	1.3:-57100807	MS.gene43987:CDS
CTTGGTCTGAGCAGCGGCGG+TGG	0.350352	1.3:+57101109	None:intergenic
TTCACAGCGGCCTTCGGCTT+CGG	0.355109	1.3:+57100651	None:intergenic
TTCGGCTTCACAACAGCTTT+AGG	0.372527	1.3:+57100591	None:intergenic
TTAGTTGCAGCCTTAGCGGC+TGG	0.381676	1.3:+57100675	None:intergenic
ACCGGAGTCTTAACACTCTT+CGG	0.394389	1.3:+57100507	None:intergenic
TTCGGCTTCACAGCGGCCTT+CGG	0.402526	1.3:+57100645	None:intergenic
TTCTTCGCCGCTTTCTTCAC+AGG	0.416978	1.3:+57100546	None:intergenic
CGGCTTAGTTGCAGCCTTAG+CGG	0.439406	1.3:+57100671	None:intergenic
AGAATGAGTTTGCGGAATGT+TGG	0.442341	1.3:+57100837	None:intergenic
GATGAAGAAGAATGAGTTTG+CGG	0.452593	1.3:+57100829	None:intergenic
GTGAGTTTGAAAGAAAGAAC+TGG	0.455638	1.3:-57100915	MS.gene43987:CDS
CACTTCTTAGCTTTCTTCAC+CGG	0.455761	1.3:+57100489	None:intergenic
ACAGCCTTAGCCTTCTTAGC+AGG	0.457075	1.3:+57100705	None:intergenic
CCGAAAGCGAAGAGTGTTAA+GGG	0.457605	1.3:-57100573	MS.gene43987:CDS
GCCGAAAGCGAAGAGTGTTA+AGG	0.460757	1.3:-57100574	MS.gene43987:CDS
CACTACTGCTCCTGCTAAGA+AGG	0.470357	1.3:-57100715	MS.gene43987:CDS
TTGGTCTGAGCAGCGGCGGT+GGG	0.488362	1.3:+57101110	None:intergenic
GGCGAAAACCGCTCCGGTGA+AGG	0.507258	1.3:-57100739	MS.gene43987:CDS
TGTGAAGAAAGCGGCGAAGA+AGG	0.511913	1.3:-57100544	MS.gene43987:CDS
GGAGCAGTAGTGGCCTTCAC+CGG	0.512024	1.3:+57100726	None:intergenic
AGCAGCGGCGGTGGGAGACA+TGG	0.520541	1.3:+57101118	None:intergenic
AAACTCCTTCAAAATTGCTC+CGG	0.521510	1.3:-57100766	MS.gene43987:CDS
TGAATTTCGTTATCGCGTGT+TGG	0.523300	1.3:+57100886	None:intergenic
CTTCAAAATTGCTCCGGCGG+CGG	0.525336	1.3:-57100760	MS.gene43987:CDS
CTTCTTAGCAGGAGCAGTAG+TGG	0.533775	1.3:+57100716	None:intergenic
ATTCATCGAAGAGAAGCATA+AGG	0.536890	1.3:-57100868	MS.gene43987:CDS
TGTTACTAAGCCAGCCGCTA+AGG	0.548744	1.3:-57100685	MS.gene43987:CDS
TGCCGCAAAGCCAAAAGCAG+CGG	0.551299	1.3:-57100619	MS.gene43987:CDS
TGCTCCTGCTAAGAAGGCTA+AGG	0.552889	1.3:-57100709	MS.gene43987:CDS
GAGAGGCTTCTTTGCTGCAG+CGG	0.555043	1.3:+57101070	None:intergenic
GGGCTTGGTCTGAGCAGCGG+CGG	0.555862	1.3:+57101106	None:intergenic
GGCGGCGGCGAAAACCGCTC+CGG	0.557405	1.3:-57100745	MS.gene43987:CDS
GTTATTACCTCAGCGTAGGT+AGG	0.578831	1.3:+57101041	None:intergenic
TCTTCATCTGAAGAAATCTG+TGG	0.580993	1.3:-57100814	MS.gene43987:CDS
CTTGGGCTTGGTCTGAGCAG+CGG	0.582303	1.3:+57101103	None:intergenic
TGCAACTAAGCCGAAGCCGA+AGG	0.584073	1.3:-57100661	MS.gene43987:CDS
GGCTTCTGGCAAGCTCGTGA+AGG	0.588674	1.3:-57100793	MS.gene43987:CDS
GCCGAAGAGTGTTAAGACTC+CGG	0.591306	1.3:-57100508	MS.gene43987:CDS
ATTTGTTATTACCTCAGCGT+AGG	0.593667	1.3:+57101037	None:intergenic
CTCCTTCAAAATTGCTCCGG+CGG	0.596987	1.3:-57100763	MS.gene43987:CDS
GAAGAAAGCGGCGAAGAAGG+TGG	0.636706	1.3:-57100541	MS.gene43987:CDS
AGTAGTGGCCTTCACCGGAG+CGG	0.652910	1.3:+57100731	None:intergenic
GCGTAGGTAGGATGAGAGAG+AGG	0.655352	1.3:+57101053	None:intergenic
CTCTCATCCTACCTACGCTG+AGG	0.671811	1.3:-57101048	MS.gene43987:intron
GGCAGCTTTCGGCTTCACAG+CGG	0.683123	1.3:+57100638	None:intergenic
GGGTACGCCTGTGAAGAAAG+CGG	0.711847	1.3:-57100553	MS.gene43987:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAACTCCTTCAAAATTGCTC+CGG	-	chr1.3:57100838-57100857	MS.gene43987:CDS	35.0%
	GATGAAGAAGAATGAGTTTG+CGG	+	chr1.3:57100778-57100797	None:intergenic	35.0%
	GTGAGTTTGAAAGAAAGAAC+TGG	-	chr1.3:57100689-57100708	MS.gene43987:CDS	35.0%
	TCTTCATCTGAAGAAATCTG+TGG	-	chr1.3:57100790-57100809	MS.gene43987:CDS	35.0%
!	ATTCATCGAAGAGAAGCATA+AGG	-	chr1.3:57100736-57100755	MS.gene43987:CDS	35.0%
!!	ATTTGTTATTACCTCAGCGT+AGG	+	chr1.3:57100570-57100589	None:intergenic	35.0%
	AGAATGAGTTTGCGGAATGT+TGG	+	chr1.3:57100770-57100789	None:intergenic	40.0%
	TGAATTTCGTTATCGCGTGT+TGG	+	chr1.3:57100721-57100740	None:intergenic	40.0%
!	CACTTCTTAGCTTTCTTCAC+CGG	+	chr1.3:57101118-57101137	None:intergenic	40.0%
	ACCGGAGTCTTAACACTCTT+CGG	+	chr1.3:57101100-57101119	None:intergenic	45.0%
	CCCTTAACACTCTTCGCTTT+CGG	+	chr1.3:57101034-57101053	None:intergenic	45.0%
	CTGAAGAAATCTGTGGCTTC+TGG	-	chr1.3:57100797-57100816	MS.gene43987:CDS	45.0%
	GTTATTACCTCAGCGTAGGT+AGG	+	chr1.3:57100566-57100585	None:intergenic	45.0%
	TTCGGCTTCACAACAGCTTT+AGG	+	chr1.3:57101016-57101035	None:intergenic	45.0%
!	TTTGCTGCAGCGGTTTTCTT+GGG	+	chr1.3:57100527-57100546	None:intergenic	45.0%
!!	CCGAAAGCGAAGAGTGTTAA+GGG	-	chr1.3:57101031-57101050	MS.gene43987:intron	45.0%
	CACTACTGCTCCTGCTAAGA+AGG	-	chr1.3:57100889-57100908	MS.gene43987:CDS	50.0%
	CTCCTTCAAAATTGCTCCGG+CGG	-	chr1.3:57100841-57100860	MS.gene43987:CDS	50.0%
	CTTCTTAGCAGGAGCAGTAG+TGG	+	chr1.3:57100891-57100910	None:intergenic	50.0%
	TGCTCCTGCTAAGAAGGCTA+AGG	-	chr1.3:57100895-57100914	MS.gene43987:CDS	50.0%
	TGTGAAGAAAGCGGCGAAGA+AGG	-	chr1.3:57101060-57101079	MS.gene43987:CDS	50.0%
	TGTTACTAAGCCAGCCGCTA+AGG	-	chr1.3:57100919-57100938	MS.gene43987:CDS	50.0%
!	ACAGCCTTAGCCTTCTTAGC+AGG	+	chr1.3:57100902-57100921	None:intergenic	50.0%
!	CTTTGCTGCAGCGGTTTTCT+TGG	+	chr1.3:57100528-57100547	None:intergenic	50.0%
!	TTCTTCGCCGCTTTCTTCAC+AGG	+	chr1.3:57101061-57101080	None:intergenic	50.0%
!!	GCCGAAAGCGAAGAGTGTTA+AGG	-	chr1.3:57101030-57101049	MS.gene43987:intron	50.0%
!!	GCCGAAGAGTGTTAAGACTC+CGG	-	chr1.3:57101096-57101115	MS.gene43987:CDS	50.0%
!!	TTTGGCTTTGCGGCAGCTTT+CGG	+	chr1.3:57100980-57100999	None:intergenic	50.0%
	CGGCTTAGTTGCAGCCTTAG+CGG	+	chr1.3:57100936-57100955	None:intergenic	55.0%
	CTCTCATCCTACCTACGCTG+AGG	-	chr1.3:57100556-57100575	MS.gene43987:CDS	55.0%
	CTTCAAAATTGCTCCGGCGG+CGG	-	chr1.3:57100844-57100863	MS.gene43987:CDS	55.0%
	GAAGAAAGCGGCGAAGAAGG+TGG	-	chr1.3:57101063-57101082	MS.gene43987:CDS	55.0%
	GAGAGGCTTCTTTGCTGCAG+CGG	+	chr1.3:57100537-57100556	None:intergenic	55.0%
	GCGTAGGTAGGATGAGAGAG+AGG	+	chr1.3:57100554-57100573	None:intergenic	55.0%
	GGGTACGCCTGTGAAGAAAG+CGG	-	chr1.3:57101051-57101070	MS.gene43987:CDS	55.0%
	TGCAACTAAGCCGAAGCCGA+AGG	-	chr1.3:57100943-57100962	MS.gene43987:intron	55.0%
	TGCCGCAAAGCCAAAAGCAG+CGG	-	chr1.3:57100985-57101004	MS.gene43987:intron	55.0%
	TTAGTTGCAGCCTTAGCGGC+TGG	+	chr1.3:57100932-57100951	None:intergenic	55.0%
!	GCAGCGGTTTTCTTGGGCTT+GGG	+	chr1.3:57100521-57100540	None:intergenic	55.0%
!	GGTTTTCTTGGGCTTGGGCT+TGG	+	chr1.3:57100516-57100535	None:intergenic	55.0%
!	TGCAGCGGTTTTCTTGGGCT+TGG	+	chr1.3:57100522-57100541	None:intergenic	55.0%
!!	TTAGGCTTCGCCGCTGCTTT+TGG	+	chr1.3:57100998-57101017	None:intergenic	55.0%
	AGTAGTGGCCTTCACCGGAG+CGG	+	chr1.3:57100876-57100895	None:intergenic	60.0%
	GGAGCAGTAGTGGCCTTCAC+CGG	+	chr1.3:57100881-57100900	None:intergenic	60.0%
	GGCAGCTTTCGGCTTCACAG+CGG	+	chr1.3:57100969-57100988	None:intergenic	60.0%
	GGCTTCTGGCAAGCTCGTGA+AGG	-	chr1.3:57100811-57100830	MS.gene43987:CDS	60.0%
	TTCACAGCGGCCTTCGGCTT+CGG	+	chr1.3:57100956-57100975	None:intergenic	60.0%
	TTCGGCTTCACAGCGGCCTT+CGG	+	chr1.3:57100962-57100981	None:intergenic	60.0%
!	CGCCGCCGGAGCAATTTTGA+AGG	+	chr1.3:57100846-57100865	None:intergenic	60.0%
!	CTTGGGCTTGGTCTGAGCAG+CGG	+	chr1.3:57100504-57100523	None:intergenic	60.0%
!!!	CGCCGCTGCTTTTGGCTTTG+CGG	+	chr1.3:57100990-57101009	None:intergenic	60.0%
	GGCGAAAACCGCTCCGGTGA+AGG	-	chr1.3:57100865-57100884	MS.gene43987:CDS	65.0%
	TTGGTCTGAGCAGCGGCGGT+GGG	+	chr1.3:57100497-57100516	None:intergenic	65.0%
	CTTGGTCTGAGCAGCGGCGG+TGG	+	chr1.3:57100498-57100517	None:intergenic	70.0%
!	GGGCTTGGTCTGAGCAGCGG+CGG	+	chr1.3:57100501-57100520	None:intergenic	70.0%
	GGCGGCGGCGAAAACCGCTC+CGG	-	chr1.3:57100859-57100878	MS.gene43987:CDS	75.0%
!	GGAGCGGTTTTCGCCGCCGC+CGG	+	chr1.3:57100860-57100879	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	57100488	57101138	57100488	ID=MS.gene43987
chr1.3	mRNA	57100488	57101138	57100488	ID=MS.gene43987.t1;Parent=MS.gene43987
chr1.3	exon	57101049	57101138	57101049	ID=MS.gene43987.t1.exon1;Parent=MS.gene43987.t1
chr1.3	CDS	57101049	57101138	57101049	ID=cds.MS.gene43987.t1;Parent=MS.gene43987.t1
chr1.3	exon	57100488	57100955	57100488	ID=MS.gene43987.t1.exon2;Parent=MS.gene43987.t1
chr1.3	CDS	57100488	57100955	57100488	ID=cds.MS.gene43987.t1;Parent=MS.gene43987.t1

Gene Sequence

>MS.gene43987

ATGTCTCCCACCGCCGCTGCTCAGACCAAGCCCAAGCCCAAGAAAACCGCTGCAGCAAAGAAGCCTCTCTCTCATCCTACCTACGCTGAGGTAATAACAAATCTCAATTTTCAAATTTTGTCTTAATTTTCTTAATTTTTGAAATTTGAATTCTGATTTTGTTTTTTTTTTTTTTTTTTGTAGATGATAACTGAAGCAATTGTGAGTTTGAAAGAAAGAACTGGTTCAAGCCAACACGCGATAACGAAATTCATCGAAGAGAAGCATAAGGATTTATCTCCAACATTCCGCAAACTCATTCTTCTTCATCTGAAGAAATCTGTGGCTTCTGGCAAGCTCGTGAAGGTGAAAAACTCCTTCAAAATTGCTCCGGCGGCGGCGAAAACCGCTCCGGTGAAGGCCACTACTGCTCCTGCTAAGAAGGCTAAGGCTGTTACTAAGCCAGCCGCTAAGGCTGCAACTAAGCCGAAGCCGAAGGCCGCTGTGAAGCCGAAAGCTGCCGCAAAGCCAAAAGCAGCGGCGAAGCCTAAAGCTGTTGTGAAGCCGAAAGCGAAGAGTGTTAAGGGTACGCCTGTGAAGAAAGCGGCGAAGAAGGTGGTTGCGAAGAAGCCGAAGAGTGTTAAGACTCCGGTGAAGAAAGCTAAGAAGTGA

Protein sequence

>MS.gene43987.t1

MSPTAAAQTKPKPKKTAAAKKPLSHPTYAEMITEAIVSLKERTGSSQHAITKFIEEKHKDLSPTFRKLILLHLKKSVASGKLVKVKNSFKIAPAAAKTAPVKATTAPAKKAKAVTKPAAKAATKPKPKAAVKPKAAAKPKAAAKPKAVVKPKAKSVKGTPVKKAAKKVVAKKPKSVKTPVKKAKK