AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44131


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44131.t1 MTR_1g083380 98.810 168 2 0 1 168 1 168 2.09e-120 337
MS.gene44131.t1 MTR_1g083350 98.214 168 3 0 1 168 1 168 1.74e-119 334
MS.gene44131.t1 MTR_2g436620 86.310 168 22 1 1 168 1 167 9.72e-103 292
MS.gene44131.t1 MTR_1g083340 91.083 157 4 1 1 147 1 157 6.42e-98 280
MS.gene44131.t1 MTR_6g071090 81.366 161 29 1 1 161 1 160 3.31e-90 260
MS.gene44131.t1 MTR_2g436620 85.211 142 20 1 27 168 1 141 2.61e-84 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44131.t1 AT3G16640 78.571 168 36 0 1 168 1 168 4.91e-96 275
MS.gene44131.t1 AT3G05540 79.882 169 32 2 1 168 1 168 1.60e-89 259

Find 43 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATATTGTTGACACTTTC+AGG 0.110440 1.3:-58927236 MS.gene44131:CDS
AATTGTAGGTCCTTGAGTTT+AGG 0.253357 1.3:+58927072 None:intergenic
TCCATTCTCAATTTCCTTGC+AGG 0.264878 1.3:+58927502 None:intergenic
ATTGTAGGTCCTTGAGTTTA+GGG 0.271566 1.3:+58927073 None:intergenic
GTATACCAGGATCTTCTTAC+AGG 0.300413 1.3:-58928389 MS.gene44131:intron
TGTTGACACTTTCAGGCTTC+AGG 0.310211 1.3:-58927229 MS.gene44131:CDS
GTTGCTGACATCTAAATTAG+AGG 0.319278 1.3:-58927151 MS.gene44131:CDS
CTACTTTGCCTATGGCTTGA+AGG 0.378916 1.3:-58926664 MS.gene44131:CDS
AACAATATCAACCACTTTGA+CGG 0.381711 1.3:+58927248 None:intergenic
GATGAAATACATACAATTGT+AGG 0.387035 1.3:+58927058 None:intergenic
AATTGCTTCTTATCAAAAGC+TGG 0.428621 1.3:+58927201 None:intergenic
CCTTGTGTTTGCTTACTACA+AGG 0.455642 1.3:-58926712 MS.gene44131:CDS
GAATGGAATGTTGTGGGAAG+TGG 0.459626 1.3:-58927486 MS.gene44131:CDS
CCATTCTCAATTTCCTTGCA+GGG 0.465811 1.3:+58927503 None:intergenic
TAGGCAAAGTAGAGAAAAGT+TGG 0.474181 1.3:+58926675 None:intergenic
TCGTCTCCACCCTCTGCAGA+AGG 0.482179 1.3:+58927294 None:intergenic
TATTTGCAGTGGGTTGTTCA+AGG 0.483236 1.3:-58927345 MS.gene44131:intron
CCCTGCAAGGAAATTGAGAA+TGG 0.490398 1.3:-58927503 MS.gene44131:CDS
CTTGATTTCCTTCAAGCCAT+AGG 0.498561 1.3:+58926656 None:intergenic
GGAGCAGTGGATGTGAATAT+TGG 0.501116 1.3:-58927324 MS.gene44131:CDS
TGCAACCATGCTTGTATACC+AGG 0.506466 1.3:-58928402 None:intergenic
GGTGCTAACCCTTCTGCAGA+GGG 0.509467 1.3:-58927303 MS.gene44131:CDS
CTCTGACTCATTCCCCTGCA+AGG 0.534349 1.3:-58927516 MS.gene44131:CDS
GTGTTTGCTTACTACAAGGA+TGG 0.538874 1.3:-58926708 MS.gene44131:CDS
GGTCCTTGAGTTTAGGGAGA+AGG 0.545288 1.3:+58927079 None:intergenic
GTTCCTTCTCCCTAAACTCA+AGG 0.563294 1.3:-58927082 MS.gene44131:CDS
GGGGAGAGCATGCATGATGA+TGG 0.565967 1.3:-58926738 MS.gene44131:CDS
CATTCTCAATTTCCTTGCAG+GGG 0.578074 1.3:+58927504 None:intergenic
CGTCTCCACCCTCTGCAGAA+GGG 0.579433 1.3:+58927295 None:intergenic
ATTTACCTGTAAGAAGATCC+TGG 0.582754 1.3:+58928384 None:intergenic
GTTGTGGGAAGTGGAAGGAA+AGG 0.585239 1.3:-58927477 MS.gene44131:intron
AATTGAGAATGGAATGTTGT+GGG 0.593952 1.3:-58927492 MS.gene44131:CDS
TTCTGCAGAGGGTGGAGACG+AGG 0.598044 1.3:-58927292 MS.gene44131:CDS
GCTAACCCTTCTGCAGAGGG+TGG 0.604009 1.3:-58927300 MS.gene44131:CDS
GTAGAGAAAAGTTGGATCAG+TGG 0.605758 1.3:+58926683 None:intergenic
GAGGGTGGAGACGAGGATGA+CGG 0.612656 1.3:-58927285 MS.gene44131:CDS
AAGATCCTGGTATACAAGCA+TGG 0.618624 1.3:+58928397 None:intergenic
TGGTGCTAACCCTTCTGCAG+AGG 0.618744 1.3:-58927304 MS.gene44131:CDS
CCTTGTAGTAAGCAAACACA+AGG 0.635355 1.3:+58926712 None:intergenic
GTGGGTTGTTCAAGGAGCAG+TGG 0.636439 1.3:-58927337 MS.gene44131:CDS
GGAATGTTGTGGGAAGTGGA+AGG 0.687071 1.3:-58927482 MS.gene44131:CDS
TGATGATCAAGCCGTCAAAG+TGG 0.714042 1.3:-58927259 MS.gene44131:CDS
AAATTGAGAATGGAATGTTG+TGG 0.718062 1.3:-58927493 MS.gene44131:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAAAGATTATTTATTTAT+GGG + chr1.3:58928254-58928273 None:intergenic 10.0%
!! AATGTAGTATTAAATGATTA+AGG + chr1.3:58926995-58927014 None:intergenic 15.0%
!! CAGTAAAGATTATTTATTTA+TGG + chr1.3:58928255-58928274 None:intergenic 15.0%
!! CTAATTTATGAACATATATT+TGG + chr1.3:58927652-58927671 None:intergenic 15.0%
!!! AATTATTTAAAACTAAAGTG+AGG + chr1.3:58926838-58926857 None:intergenic 15.0%
!!! ATATTAGAGATTATTGTTTT+TGG - chr1.3:58927438-58927457 MS.gene44131:intron 15.0%
!!! TTATCTTTCATCTTTTTATT+TGG - chr1.3:58928011-58928030 MS.gene44131:intron 15.0%
!! CTTAATCTAATACTTGATTA+TGG - chr1.3:58927383-58927402 MS.gene44131:intron 20.0%
!! TAAATATATAGGGGTAATAT+AGG - chr1.3:58927037-58927056 MS.gene44131:intron 20.0%
!! TAAGAGATAATCATGTTTAA+GGG + chr1.3:58927367-58927386 None:intergenic 20.0%
!! TAGAAAGAAACATTATAGAA+AGG + chr1.3:58927608-58927627 None:intergenic 20.0%
!! TTAAGAGATAATCATGTTTA+AGG + chr1.3:58927368-58927387 None:intergenic 20.0%
!!! ATAAAAACAGAGAAGTTTTA+AGG - chr1.3:58927209-58927228 MS.gene44131:CDS 20.0%
!!! CTTTTTTGTGGATAAATATA+GGG - chr1.3:58928050-58928069 MS.gene44131:intron 20.0%
!!! TAAAACTTCTCTGTTTTTAT+GGG + chr1.3:58927209-58927228 None:intergenic 20.0%
!!! TCTTTTTTGTGGATAAATAT+AGG - chr1.3:58928049-58928068 MS.gene44131:intron 20.0%
!!! TTAAAACTTCTCTGTTTTTA+TGG + chr1.3:58927210-58927229 None:intergenic 20.0%
! AAAAATCTCAAAGATGATGA+TGG + chr1.3:58926687-58926706 None:intergenic 25.0%
! ATGCAAAAGTGACAATTATA+AGG - chr1.3:58927123-58927142 MS.gene44131:CDS 25.0%
! ATTACACAACAATCAACTTT+AGG + chr1.3:58927675-58927694 None:intergenic 25.0%
! CTCTCTGCAATAAATATATA+GGG - chr1.3:58927027-58927046 MS.gene44131:intron 25.0%
! GAAATTCATTCACTAAATAC+AGG + chr1.3:58926779-58926798 None:intergenic 25.0%
! GATGAAATACATACAATTGT+AGG + chr1.3:58927990-58928009 None:intergenic 25.0%
! TCGAAATGTGACAATTAAAA+AGG + chr1.3:58926899-58926918 None:intergenic 25.0%
! TCTCTCTGCAATAAATATAT+AGG - chr1.3:58927026-58927045 MS.gene44131:intron 25.0%
! TCTCTGCAATAAATATATAG+GGG - chr1.3:58927028-58927047 MS.gene44131:intron 25.0%
! TGCAAAAGTGACAATTATAA+GGG - chr1.3:58927124-58927143 MS.gene44131:CDS 25.0%
! TTGTGACAGGATTAAATTTA+TGG - chr1.3:58927308-58927327 MS.gene44131:CDS 25.0%
!! GTTCAAAGTATCATTAAATG+TGG + chr1.3:58927068-58927087 None:intergenic 25.0%
!! TCGTTTGTATTTCTTTTCAT+AGG - chr1.3:58926718-58926737 MS.gene44131:CDS 25.0%
!!! AGTAAAAGTATTTGTTGTCT+TGG + chr1.3:58926935-58926954 None:intergenic 25.0%
!!! ATTAAGTTCTCTCTTTTTTG+TGG - chr1.3:58928038-58928057 MS.gene44131:intron 25.0%
AAATTGAGAATGGAATGTTG+TGG - chr1.3:58927552-58927571 MS.gene44131:intron 30.0%
AACAATATCAACCACTTTGA+CGG + chr1.3:58927800-58927819 None:intergenic 30.0%
AAGTGACAATTATAAGGGAA+CGG - chr1.3:58927129-58927148 MS.gene44131:CDS 30.0%
AATTGAGAATGGAATGTTGT+GGG - chr1.3:58927553-58927572 MS.gene44131:intron 30.0%
AATTGCTTCTTATCAAAAGC+TGG + chr1.3:58927847-58927866 None:intergenic 30.0%
CTTTGCTGATGAATTTCTTT+AGG - chr1.3:58927494-58927513 MS.gene44131:CDS 30.0%
GACAATTAAAAAGGAACAGA+GGG + chr1.3:58926890-58926909 None:intergenic 30.0%
GTAATGTGATTATTTGCAGT+GGG - chr1.3:58927690-58927709 MS.gene44131:intron 30.0%
TGACAATTAAAAAGGAACAG+AGG + chr1.3:58926891-58926910 None:intergenic 30.0%
TGTAATGTGATTATTTGCAG+TGG - chr1.3:58927689-58927708 MS.gene44131:intron 30.0%
TTGATATTGTTGACACTTTC+AGG - chr1.3:58927809-58927828 MS.gene44131:intron 30.0%
! TTATGTTGTTTGTGTTCTGT+TGG - chr1.3:58927253-58927272 MS.gene44131:CDS 30.0%
!! AATCAATGTTGCAGTTTTGT+TGG - chr1.3:58928286-58928305 MS.gene44131:intron 30.0%
!!! ATCAATGTTGCAGTTTTGTT+GGG - chr1.3:58928287-58928306 MS.gene44131:intron 30.0%
!!! TTATTGTTTTTGGTTGCTGA+TGG - chr1.3:58927448-58927467 MS.gene44131:intron 30.0%
AATTGTAGGTCCTTGAGTTT+AGG + chr1.3:58927976-58927995 None:intergenic 35.0%
ATTGTAGGTCCTTGAGTTTA+GGG + chr1.3:58927975-58927994 None:intergenic 35.0%
ATTTACCTGTAAGAAGATCC+TGG + chr1.3:58926664-58926683 None:intergenic 35.0%
GTTGCTGACATCTAAATTAG+AGG - chr1.3:58927894-58927913 MS.gene44131:intron 35.0%
TAGGCAAAGTAGAGAAAAGT+TGG + chr1.3:58928373-58928392 None:intergenic 35.0%
! ACTTTTCTCTACTTTGCCTA+TGG - chr1.3:58928373-58928392 MS.gene44131:intron 35.0%
!! AAATGTTAACTTGTGCCCTT+GGG - chr1.3:58928073-58928092 MS.gene44131:intron 35.0%
!!! TCAATGTTGCAGTTTTGTTG+GGG - chr1.3:58928288-58928307 MS.gene44131:intron 35.0%
CATTCTCAATTTCCTTGCAG+GGG + chr1.3:58927544-58927563 None:intergenic 40.0%
CCATTCTCAATTTCCTTGCA+GGG + chr1.3:58927545-58927564 None:intergenic 40.0%
CCTTGTAGTAAGCAAACACA+AGG + chr1.3:58928336-58928355 None:intergenic 40.0%
CCTTGTGTTTGCTTACTACA+AGG - chr1.3:58928333-58928352 MS.gene44131:intron 40.0%
CTTCTTAACTTATGCCCCAA+GGG + chr1.3:58928091-58928110 None:intergenic 40.0%
CTTGATTTCCTTCAAGCCAT+AGG + chr1.3:58928392-58928411 None:intergenic 40.0%
GACAATTATAAGGGAACGGA+GGG - chr1.3:58927133-58927152 MS.gene44131:CDS 40.0%
GTAGAGAAAAGTTGGATCAG+TGG + chr1.3:58928365-58928384 None:intergenic 40.0%
GTATACCAGGATCTTCTTAC+AGG - chr1.3:58926656-58926675 MS.gene44131:CDS 40.0%
GTGTTTGCTTACTACAAGGA+TGG - chr1.3:58928337-58928356 MS.gene44131:intron 40.0%
TCCATTCTCAATTTCCTTGC+AGG + chr1.3:58927546-58927565 None:intergenic 40.0%
TCTTCTTAACTTATGCCCCA+AGG + chr1.3:58928092-58928111 None:intergenic 40.0%
TGACAATTATAAGGGAACGG+AGG - chr1.3:58927132-58927151 MS.gene44131:CDS 40.0%
TGTTGTGTTTGTGTTGTGAC+AGG - chr1.3:58927295-58927314 MS.gene44131:CDS 40.0%
!! AATGTTAACTTGTGCCCTTG+GGG - chr1.3:58928074-58928093 MS.gene44131:intron 40.0%
!! CATGTTAACTTGTGCCCTAA+GGG + chr1.3:58928230-58928249 None:intergenic 40.0%
!! GAAATGTTAACTTGTGCCCT+TGG - chr1.3:58928072-58928091 MS.gene44131:intron 40.0%
!! TATTTGCAGTGGGTTGTTCA+AGG - chr1.3:58927700-58927719 MS.gene44131:intron 40.0%
!! TCATGTTAACTTGTGCCCTA+AGG + chr1.3:58928231-58928250 None:intergenic 40.0%
CCCTGCAAGGAAATTGAGAA+TGG - chr1.3:58927542-58927561 MS.gene44131:intron 45.0%
CTACTTTGCCTATGGCTTGA+AGG - chr1.3:58928381-58928400 MS.gene44131:intron 45.0%
GAATGGAATGTTGTGGGAAG+TGG - chr1.3:58927559-58927578 MS.gene44131:intron 45.0%
GGAGCAGTGGATGTGAATAT+TGG - chr1.3:58927721-58927740 MS.gene44131:intron 45.0%
GTTCCTTCTCCCTAAACTCA+AGG - chr1.3:58927963-58927982 MS.gene44131:intron 45.0%
TGATGATCAAGCCGTCAAAG+TGG - chr1.3:58927786-58927805 MS.gene44131:intron 45.0%
TGTTGACACTTTCAGGCTTC+AGG - chr1.3:58927816-58927835 MS.gene44131:intron 45.0%
!! CTCCTTAACTTGTGCCCTTA+GGG - chr1.3:58928213-58928232 MS.gene44131:intron 45.0%
!! TCTCCTTAACTTGTGCCCTT+AGG - chr1.3:58928212-58928231 MS.gene44131:intron 45.0%
GGAATGTTGTGGGAAGTGGA+AGG - chr1.3:58927563-58927582 MS.gene44131:intron 50.0%
GGGAAGTGGAAGGAAAGGTT+AGG - chr1.3:58927573-58927592 MS.gene44131:intron 50.0%
GGTCCTTGAGTTTAGGGAGA+AGG + chr1.3:58927969-58927988 None:intergenic 50.0%
GTTGTGGGAAGTGGAAGGAA+AGG - chr1.3:58927568-58927587 MS.gene44131:intron 50.0%
! TGCCCTAAGGGCACAAGTTA+AGG + chr1.3:58928218-58928237 None:intergenic 50.0%
CTCTGACTCATTCCCCTGCA+AGG - chr1.3:58927529-58927548 MS.gene44131:CDS 55.0%
GGTGCTAACCCTTCTGCAGA+GGG - chr1.3:58927742-58927761 MS.gene44131:intron 55.0%
! GGGGAGAGCATGCATGATGA+TGG - chr1.3:58928307-58928326 MS.gene44131:intron 55.0%
! GTGGGTTGTTCAAGGAGCAG+TGG - chr1.3:58927708-58927727 MS.gene44131:intron 55.0%
! TGGTGCTAACCCTTCTGCAG+AGG - chr1.3:58927741-58927760 MS.gene44131:intron 55.0%
CGTCTCCACCCTCTGCAGAA+GGG + chr1.3:58927753-58927772 None:intergenic 60.0%
GAGGGTGGAGACGAGGATGA+CGG - chr1.3:58927760-58927779 MS.gene44131:intron 60.0%
GCTAACCCTTCTGCAGAGGG+TGG - chr1.3:58927745-58927764 MS.gene44131:intron 60.0%
TCGTCTCCACCCTCTGCAGA+AGG + chr1.3:58927754-58927773 None:intergenic 60.0%
TTCTGCAGAGGGTGGAGACG+AGG - chr1.3:58927753-58927772 MS.gene44131:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 58926650 58928417 58926650 ID=MS.gene44131
chr1.3 mRNA 58926650 58928417 58926650 ID=MS.gene44131.t1;Parent=MS.gene44131
chr1.3 exon 58928390 58928417 58928390 ID=MS.gene44131.t1.exon1;Parent=MS.gene44131.t1
chr1.3 CDS 58928390 58928417 58928390 ID=cds.MS.gene44131.t1;Parent=MS.gene44131.t1
chr1.3 exon 58927478 58927551 58927478 ID=MS.gene44131.t1.exon2;Parent=MS.gene44131.t1
chr1.3 CDS 58927478 58927551 58927478 ID=cds.MS.gene44131.t1;Parent=MS.gene44131.t1
chr1.3 exon 58927072 58927358 58927072 ID=MS.gene44131.t1.exon3;Parent=MS.gene44131.t1
chr1.3 CDS 58927072 58927358 58927072 ID=cds.MS.gene44131.t1;Parent=MS.gene44131.t1
chr1.3 exon 58926650 58926767 58926650 ID=MS.gene44131.t1.exon4;Parent=MS.gene44131.t1
chr1.3 CDS 58926650 58926767 58926650 ID=cds.MS.gene44131.t1;Parent=MS.gene44131.t1
Gene Sequence

>MS.gene44131

ATGCTTGTATACCAGGATCTTCTTACAGGTAAATCCATCATCATCTTTGAGATTTTTTGCTTTAGTTTTCGTTTGTATTTCTTTTCATAGGATTTTGATATTGTGATGAATATTGAATACTTGTTTCCTGTATTTAGTGAATGAATTTCTTGTTATGTTTTTATGCATATGTTGTTCATAATAAACCTCACTTTAGTTTTAAATAATTCTTCTTTATCTTGTTAGATAATTAATACTCCCTCTGTTCCTTTTTAATTGTCACATTTCGACTTTTTACACAAACCAAGACAACAAATACTTTTACTACTTTTGATACAACAATTTATCTTTTTTCTATAATAACCTTAATCATTTAATACTACATTTCATACATTTCTCTCTCTGCAATAAATATATAGGGGTAATATAGGTAAAACCACATTTAATGATACTTTGAACTTTGAAAATGACAGTTATTTAGAAACAAAAATTTTATGCAAAAGTGACAATTATAAGGGAACGGAGGGAGTATTTTTCTAGCTGTAGAGTTTTTTTTTTTTTTTGTGATGTGTTTTGTCCCATAAAAACAGAGAAGTTTTAAGGTTTTCAAAAGAATATGAAATTTTATGTTGTTTGTGTTCTGTTGGTCTAATTTAAAGTAAAAGTTGTTGTGTTTGTGTTGTGACAGGATTAAATTTATGGTTTTATGACAAAGTGCTAAATTAATATTTTCAACCCTTAAACATGATTATCTCTTAATCTAATACTTGATTATGGATTTTAGTTTCTGAGATTGCTAACTGCTTCAAATATTAGAGATTATTGTTTTTGGTTGCTGATGGATTGAGTGAAATAATGTTTCTAACTTTGCTGATGAATTTCTTTAGGTGATGAGCTTCTCTCTGACTCATTCCCCTGCAAGGAAATTGAGAATGGAATGTTGTGGGAAGTGGAAGGAAAGGTTAGGAACAAATTTCCTTTCTATAATGTTTCTTTCTATATGCTTTATTTTTCATGTCACCAAATATATGTTCATAAATTAGCCTAAAGTTGATTGTTGTGTAATGTGATTATTTGCAGTGGGTTGTTCAAGGAGCAGTGGATGTGAATATTGGTGCTAACCCTTCTGCAGAGGGTGGAGACGAGGATGACGGTGTTGATGATCAAGCCGTCAAAGTGGTTGATATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGAAGCAATTTGTTACTTATATGAAAAGATATATTAAGTTGCTGACATCTAAATTAGAGGCAGAAAAGCAAGAGTTGTTCAAGAAGCACATTGAAGCAGCAACTAAGTTCCTTCTCCCTAAACTCAAGGACCTACAATTGTATGTATTTCATCATTATCTTTCATCTTTTTATTTGGTTTTATTAAGTTCTCTCTTTTTTGTGGATAAATATAGGGAAATGTTAACTTGTGCCCTTGGGGCATAAGTTAAGAAGACAAATATAGAAAGTTTTTCTTGAAACTTGTGTATTCAATGTCTAAAACGTTGCAAAAGTTATACTTTCAATGTAAAATTTATTTTTAATTTCTATTTTAATCTCCTTAACTTGTGCCCTTAGGGCACAAGTTAACATGACCCATAAATAAATAATCTTTACTGATTAAGTAATTAATCAATGTTGCAGTTTTGTTGGGGAGAGCATGCATGATGATGGAAGCCTTGTGTTTGCTTACTACAAGGATGGTGCCACTGATCCAACTTTTCTCTACTTTGCCTATGGCTTGAAGGAAATCAAGTGCTAA

Protein sequence

>MS.gene44131.t1

MLVYQDLLTGDELLSDSFPCKEIENGMLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKKQFVTYMKRYIKLLTSKLEAEKQELFKKHIEAATKFLLPKLKDLQFFVGESMHDDGSLVFAYYKDGATDPTFLYFAYGLKEIKC