AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056064


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056064.t1 MTR_1g083350 99.405 168 1 0 1 168 1 168 1.37e-121 340
MS.gene056064.t1 MTR_1g083380 98.810 168 2 0 1 168 1 168 1.12e-120 337
MS.gene056064.t1 MTR_2g436620 86.310 168 22 1 1 168 1 167 3.04e-103 293
MS.gene056064.t1 MTR_1g083340 91.720 157 3 1 1 147 1 157 9.42e-99 281
MS.gene056064.t1 MTR_6g071090 81.366 161 29 1 1 161 1 160 1.18e-90 261
MS.gene056064.t1 MTR_2g436620 84.507 142 21 1 27 168 1 141 1.27e-83 243
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056064.t1 AT3G16640 79.167 168 35 0 1 168 1 168 4.54e-97 278
MS.gene056064.t1 AT3G05540 80.473 169 31 2 1 168 1 168 3.23e-90 260

Find 42 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATATTGTTGACACTTTC+AGG 0.110440 1.1:-61650252 MS.gene056064:CDS
AATTGTAGGTCCTTGAGTTT+AGG 0.253357 1.1:+61650088 None:intergenic
ATTGTAGGTCCTTGAGTTTA+GGG 0.271566 1.1:+61650089 None:intergenic
GTATACCAGGATCTTCTTAC+AGG 0.300413 1.1:-61651131 MS.gene056064:intron
TGTTGACACTTTCAGGCTTC+AGG 0.310211 1.1:-61650245 MS.gene056064:CDS
TCCATTCTCAATTTCCTTGT+AGG 0.360282 1.1:+61650518 None:intergenic
CTACTTTGCCTATGGCTTGA+AGG 0.378916 1.1:-61649680 MS.gene056064:CDS
AACAATATCAACCACTTTGA+CGG 0.381711 1.1:+61650264 None:intergenic
GATGAAATACATACAATTGT+AGG 0.387035 1.1:+61650074 None:intergenic
CCATTCTCAATTTCCTTGTA+GGG 0.404442 1.1:+61650519 None:intergenic
AATTGCTTCTTATCAAAAGC+TGG 0.428621 1.1:+61650217 None:intergenic
CCTTGTGTTTGCTTACTACA+AGG 0.455642 1.1:-61649728 MS.gene056064:CDS
GAATGGAATGTTGTGGGAAG+TGG 0.459626 1.1:-61650502 MS.gene056064:CDS
TAGGCAAAGTAGAGAAAAGT+TGG 0.474181 1.1:+61649691 None:intergenic
CCCTACAAGGAAATTGAGAA+TGG 0.477742 1.1:-61650519 MS.gene056064:CDS
TCGTCTCCACCCTCTGCAGA+AGG 0.482179 1.1:+61650310 None:intergenic
TATTTGCAGTGGGTTGTTCA+AGG 0.483236 1.1:-61650361 MS.gene056064:intron
CTCTGACTCATTCCCCTACA+AGG 0.486361 1.1:-61650532 MS.gene056064:CDS
CTTGATTTCCTTCAAGCCAT+AGG 0.498561 1.1:+61649672 None:intergenic
GGAGCAGTGGATGTGAATAT+TGG 0.501116 1.1:-61650340 MS.gene056064:CDS
TGCAACCATGCTTGTATACC+AGG 0.506466 1.1:-61651144 None:intergenic
GGTGCTAACCCTTCTGCAGA+GGG 0.509467 1.1:-61650319 MS.gene056064:CDS
GTGTTTGCTTACTACAAGGA+TGG 0.538874 1.1:-61649724 MS.gene056064:CDS
GGTCCTTGAGTTTAGGGAGA+AGG 0.545288 1.1:+61650095 None:intergenic
CATTCTCAATTTCCTTGTAG+GGG 0.549656 1.1:+61650520 None:intergenic
GTTCCTTCTCCCTAAACTCA+AGG 0.563294 1.1:-61650098 MS.gene056064:CDS
GGGGAGAGCATGCATGATGA+TGG 0.565967 1.1:-61649754 MS.gene056064:CDS
CGTCTCCACCCTCTGCAGAA+GGG 0.579433 1.1:+61650311 None:intergenic
ATTTACCTGTAAGAAGATCC+TGG 0.582754 1.1:+61651126 None:intergenic
GTTGTGGGAAGTGGAAGGAA+AGG 0.585239 1.1:-61650493 MS.gene056064:intron
AATTGAGAATGGAATGTTGT+GGG 0.593952 1.1:-61650508 MS.gene056064:CDS
TTCTGCAGAGGGTGGAGACG+AGG 0.598044 1.1:-61650308 MS.gene056064:CDS
GCTAACCCTTCTGCAGAGGG+TGG 0.604009 1.1:-61650316 MS.gene056064:CDS
GTAGAGAAAAGTTGGATCAG+TGG 0.605758 1.1:+61649699 None:intergenic
GAGGGTGGAGACGAGGATGA+CGG 0.612656 1.1:-61650301 MS.gene056064:CDS
AAGATCCTGGTATACAAGCA+TGG 0.618624 1.1:+61651139 None:intergenic
TGGTGCTAACCCTTCTGCAG+AGG 0.618744 1.1:-61650320 MS.gene056064:CDS
CCTTGTAGTAAGCAAACACA+AGG 0.635355 1.1:+61649728 None:intergenic
GTGGGTTGTTCAAGGAGCAG+TGG 0.636439 1.1:-61650353 MS.gene056064:CDS
GGAATGTTGTGGGAAGTGGA+AGG 0.687071 1.1:-61650498 MS.gene056064:CDS
TGATGATCAAGCCGTCAAAG+TGG 0.714042 1.1:-61650275 MS.gene056064:CDS
AAATTGAGAATGGAATGTTG+TGG 0.718062 1.1:-61650509 MS.gene056064:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAGTAAATATTATTTATTTA+TGG + chr1.1:61650997-61651016 None:intergenic 10.0%
!!! AATTATTTAAAACTAAAGTG+AGG + chr1.1:61649854-61649873 None:intergenic 15.0%
!!! ATATTAGAGATTATTGTTTT+TGG - chr1.1:61650180-61650199 MS.gene056064:CDS 15.0%
!!! TTATCTTTCATCTTTTTATT+TGG - chr1.1:61650753-61650772 MS.gene056064:intron 15.0%
!! CTAATTTATGAACATACATT+TGG + chr1.1:61650394-61650413 None:intergenic 20.0%
!! CTTAATCTAATACTTGATTA+TGG - chr1.1:61650125-61650144 MS.gene056064:CDS 20.0%
!! TAAGAGATAATCATGTTTAA+GGG + chr1.1:61650109-61650128 None:intergenic 20.0%
!! TAGAAAGAAACATTATAGAA+AGG + chr1.1:61650350-61650369 None:intergenic 20.0%
!! TTAAGAGATAATCATGTTTA+AGG + chr1.1:61650110-61650129 None:intergenic 20.0%
!!! ATAAAAACAGAGAAGTTTTA+AGG - chr1.1:61649951-61649970 MS.gene056064:intron 20.0%
!!! CTTTTTTGTGGATAAATAAA+GGG - chr1.1:61650792-61650811 MS.gene056064:intron 20.0%
!!! TAAAACTTCTCTGTTTTTAT+GGG + chr1.1:61649951-61649970 None:intergenic 20.0%
!!! TCTTTTTTGTGGATAAATAA+AGG - chr1.1:61650791-61650810 MS.gene056064:intron 20.0%
!!! TTAAAACTTCTCTGTTTTTA+TGG + chr1.1:61649952-61649971 None:intergenic 20.0%
! AAAAATCTCAAAGATGATGA+TGG + chr1.1:61649703-61649722 None:intergenic 25.0%
! ATTACACAACAATCAACTTT+AGG + chr1.1:61650417-61650436 None:intergenic 25.0%
! GAAATTCATTCACTAAATAC+AGG + chr1.1:61649795-61649814 None:intergenic 25.0%
! GATGAAATACATACAATTGT+AGG + chr1.1:61650732-61650751 None:intergenic 25.0%
! TTGTGACAGGATTAAATTTA+TGG - chr1.1:61650050-61650069 MS.gene056064:intron 25.0%
!! TCGTTTGTATTTCTTTTCAT+AGG - chr1.1:61649734-61649753 MS.gene056064:CDS 25.0%
!!! ATTAAGTTCTCTCTTTTTTG+TGG - chr1.1:61650780-61650799 MS.gene056064:intron 25.0%
AAATTGAGAATGGAATGTTG+TGG - chr1.1:61650294-61650313 MS.gene056064:CDS 30.0%
AACAATATCAACCACTTTGA+CGG + chr1.1:61650542-61650561 None:intergenic 30.0%
AATTGAGAATGGAATGTTGT+GGG - chr1.1:61650295-61650314 MS.gene056064:CDS 30.0%
AATTGCTTCTTATCAAAAGC+TGG + chr1.1:61650589-61650608 None:intergenic 30.0%
CTTTGCTGATGAATTTCTTT+AGG - chr1.1:61650236-61650255 MS.gene056064:CDS 30.0%
GTAATGTGATTATTTGCAGT+GGG - chr1.1:61650432-61650451 MS.gene056064:intron 30.0%
TGTAATGTGATTATTTGCAG+TGG - chr1.1:61650431-61650450 MS.gene056064:intron 30.0%
TTGATATTGTTGACACTTTC+AGG - chr1.1:61650551-61650570 MS.gene056064:intron 30.0%
! TTATGTTGTTTGTGTTCTGT+TGG - chr1.1:61649995-61650014 MS.gene056064:intron 30.0%
!! AATCAATGTTGCAGTTTTGT+TGG - chr1.1:61651028-61651047 MS.gene056064:intron 30.0%
!!! ATCAATGTTGCAGTTTTGTT+GGG - chr1.1:61651029-61651048 MS.gene056064:intron 30.0%
!!! TTATTGTTTTTGGTTGCTGA+TGG - chr1.1:61650190-61650209 MS.gene056064:CDS 30.0%
AATTGTAGGTCCTTGAGTTT+AGG + chr1.1:61650718-61650737 None:intergenic 35.0%
ATTGTAGGTCCTTGAGTTTA+GGG + chr1.1:61650717-61650736 None:intergenic 35.0%
ATTTACCTGTAAGAAGATCC+TGG + chr1.1:61649680-61649699 None:intergenic 35.0%
CATTCTCAATTTCCTTGTAG+GGG + chr1.1:61650286-61650305 None:intergenic 35.0%
CCATTCTCAATTTCCTTGTA+GGG + chr1.1:61650287-61650306 None:intergenic 35.0%
TAGGCAAAGTAGAGAAAAGT+TGG + chr1.1:61651115-61651134 None:intergenic 35.0%
TCCATTCTCAATTTCCTTGT+AGG + chr1.1:61650288-61650307 None:intergenic 35.0%
! ACTTTTCTCTACTTTGCCTA+TGG - chr1.1:61651115-61651134 MS.gene056064:intron 35.0%
!! AAATGTTAACTTGTGCCCTT+GGG - chr1.1:61650815-61650834 MS.gene056064:intron 35.0%
!! TTGAACAACTCTTGCTTTTC+TGG + chr1.1:61650661-61650680 None:intergenic 35.0%
!!! TCAATGTTGCAGTTTTGTTG+GGG - chr1.1:61651030-61651049 MS.gene056064:intron 35.0%
CCCTACAAGGAAATTGAGAA+TGG - chr1.1:61650284-61650303 MS.gene056064:CDS 40.0%
CCTTGTAGTAAGCAAACACA+AGG + chr1.1:61651078-61651097 None:intergenic 40.0%
CCTTGTGTTTGCTTACTACA+AGG - chr1.1:61651075-61651094 MS.gene056064:intron 40.0%
CTTCTTAACTTATGCCCCAA+GGG + chr1.1:61650833-61650852 None:intergenic 40.0%
CTTGATTTCCTTCAAGCCAT+AGG + chr1.1:61651134-61651153 None:intergenic 40.0%
GTAGAGAAAAGTTGGATCAG+TGG + chr1.1:61651107-61651126 None:intergenic 40.0%
GTATACCAGGATCTTCTTAC+AGG - chr1.1:61649672-61649691 MS.gene056064:CDS 40.0%
GTGTTTGCTTACTACAAGGA+TGG - chr1.1:61651079-61651098 MS.gene056064:intron 40.0%
TCTTCTTAACTTATGCCCCA+AGG + chr1.1:61650834-61650853 None:intergenic 40.0%
TGTTGTGTTTGTGTTGTGAC+AGG - chr1.1:61650037-61650056 MS.gene056064:intron 40.0%
!! AATGTTAACTTGTGCCCTTG+GGG - chr1.1:61650816-61650835 MS.gene056064:intron 40.0%
!! CATGTTAACTTGTGCCCTAA+GGG + chr1.1:61650972-61650991 None:intergenic 40.0%
!! GAAATGTTAACTTGTGCCCT+TGG - chr1.1:61650814-61650833 MS.gene056064:intron 40.0%
!! TATTTGCAGTGGGTTGTTCA+AGG - chr1.1:61650442-61650461 MS.gene056064:intron 40.0%
!! TCATGTTAACTTGTGCCCTA+AGG + chr1.1:61650973-61650992 None:intergenic 40.0%
CTACTTTGCCTATGGCTTGA+AGG - chr1.1:61651123-61651142 MS.gene056064:intron 45.0%
GAATGGAATGTTGTGGGAAG+TGG - chr1.1:61650301-61650320 MS.gene056064:CDS 45.0%
GGAGCAGTGGATGTGAATAT+TGG - chr1.1:61650463-61650482 MS.gene056064:intron 45.0%
GTTCCTTCTCCCTAAACTCA+AGG - chr1.1:61650705-61650724 MS.gene056064:intron 45.0%
TGATGATCAAGCCGTCAAAG+TGG - chr1.1:61650528-61650547 MS.gene056064:CDS 45.0%
TGTTGACACTTTCAGGCTTC+AGG - chr1.1:61650558-61650577 MS.gene056064:intron 45.0%
!! CTCCTTAACTTGTGCCCTTA+GGG - chr1.1:61650955-61650974 MS.gene056064:intron 45.0%
!! TCTCCTTAACTTGTGCCCTT+AGG - chr1.1:61650954-61650973 MS.gene056064:intron 45.0%
!! AGTAAATATTATTTATTTAT+GGG + chr1.1:61650996-61651015 None:intergenic 5.0%
CTCTGACTCATTCCCCTACA+AGG - chr1.1:61650271-61650290 MS.gene056064:CDS 50.0%
GGAATGTTGTGGGAAGTGGA+AGG - chr1.1:61650305-61650324 MS.gene056064:CDS 50.0%
GGGAAGTGGAAGGAAAGGTT+AGG - chr1.1:61650315-61650334 MS.gene056064:CDS 50.0%
GGTCCTTGAGTTTAGGGAGA+AGG + chr1.1:61650711-61650730 None:intergenic 50.0%
GTTGTGGGAAGTGGAAGGAA+AGG - chr1.1:61650310-61650329 MS.gene056064:CDS 50.0%
! TGCCCTAAGGGCACAAGTTA+AGG + chr1.1:61650960-61650979 None:intergenic 50.0%
GGTGCTAACCCTTCTGCAGA+GGG - chr1.1:61650484-61650503 MS.gene056064:intron 55.0%
! GGGGAGAGCATGCATGATGA+TGG - chr1.1:61651049-61651068 MS.gene056064:intron 55.0%
! GTGGGTTGTTCAAGGAGCAG+TGG - chr1.1:61650450-61650469 MS.gene056064:intron 55.0%
! TGGTGCTAACCCTTCTGCAG+AGG - chr1.1:61650483-61650502 MS.gene056064:intron 55.0%
CGTCTCCACCCTCTGCAGAA+GGG + chr1.1:61650495-61650514 None:intergenic 60.0%
GAGGGTGGAGACGAGGATGA+CGG - chr1.1:61650502-61650521 MS.gene056064:CDS 60.0%
GCTAACCCTTCTGCAGAGGG+TGG - chr1.1:61650487-61650506 MS.gene056064:intron 60.0%
TCGTCTCCACCCTCTGCAGA+AGG + chr1.1:61650496-61650515 None:intergenic 60.0%
TTCTGCAGAGGGTGGAGACG+AGG - chr1.1:61650495-61650514 MS.gene056064:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 61649666 61651159 61649666 ID=MS.gene056064
chr1.1 mRNA 61649666 61651159 61649666 ID=MS.gene056064.t1;Parent=MS.gene056064
chr1.1 exon 61651132 61651159 61651132 ID=MS.gene056064.t1.exon1;Parent=MS.gene056064.t1
chr1.1 CDS 61651132 61651159 61651132 ID=cds.MS.gene056064.t1;Parent=MS.gene056064.t1
chr1.1 exon 61650494 61650567 61650494 ID=MS.gene056064.t1.exon2;Parent=MS.gene056064.t1
chr1.1 CDS 61650494 61650567 61650494 ID=cds.MS.gene056064.t1;Parent=MS.gene056064.t1
chr1.1 exon 61650088 61650374 61650088 ID=MS.gene056064.t1.exon3;Parent=MS.gene056064.t1
chr1.1 CDS 61650088 61650374 61650088 ID=cds.MS.gene056064.t1;Parent=MS.gene056064.t1
chr1.1 exon 61649666 61649783 61649666 ID=MS.gene056064.t1.exon4;Parent=MS.gene056064.t1
chr1.1 CDS 61649666 61649783 61649666 ID=cds.MS.gene056064.t1;Parent=MS.gene056064.t1
Gene Sequence

>MS.gene056064

ATGCTTGTATACCAGGATCTTCTTACAGGTAAATCCATCATCATCTTTGAGATTTTTTGCTTTAGTTTTCGTTTGTATTTCTTTTCATAGGATTTTGATATTGTGATGAATATTGAATACTTGTTTCCTGTATTTAGTGAATGAATTTCTTGTTATGTTTTTATGCATATGTTGTTCATAATAAACCTCACTTTAGTTTTAAATAATTCTTCTTTATCTTGTTAGATAATTAATATTTTTCTAGCTGTAGAGTTTTTTTTTTTTTTTTGTGATGTGTTTTGTCCCATAAAAACAGAGAAGTTTTAAGGTTTTCAAAAGAATATGAAATTTTATGTTGTTTGTGTTCTGTTGGTCTAATTTAAAGTAAAAGTTGTTGTGTTTGTGTTGTGACAGGATTAAATTTATGGTTTTATGACAAAGTGCTAAATTAATATTTTCAACCCTTAAACATGATTATCTCTTAATCTAATACTTGATTATGGATTTTAGTTTCTGAGATTGCTAACTGCTTCAAATATTAGAGATTATTGTTTTTGGTTGCTGATGGATTGAGTGAAATAATGTTTCTAACTTTGCTGATGAATTTCTTTAGGTGATGAGCTTCTCTCTGACTCATTCCCCTACAAGGAAATTGAGAATGGAATGTTGTGGGAAGTGGAAGGAAAGGTTAGGAACAAATTTCCTTTCTATAATGTTTCTTTCTATATGCTTTATTTTTCATGTCACCAAATGTATGTTCATAAATTAGCCTAAAGTTGATTGTTGTGTAATGTGATTATTTGCAGTGGGTTGTTCAAGGAGCAGTGGATGTGAATATTGGTGCTAACCCTTCTGCAGAGGGTGGAGACGAGGATGACGGTGTTGATGATCAAGCCGTCAAAGTGGTTGATATTGTTGACACTTTCAGGCTTCAGGAGCAACCAGCTTTTGATAAGAAGCAATTTGTTACTTATATGAAAAGATATATTAAGTTGCTGACATCTAAATTAGAGCCAGAAAAGCAAGAGTTGTTCAAGAAGCACATTGAAGCAGCAACTAAGTTCCTTCTCCCTAAACTCAAGGACCTACAATTGTATGTATTTCATCATTATCTTTCATCTTTTTATTTGGTTTTATTAAGTTCTCTCTTTTTTGTGGATAAATAAAGGGAAATGTTAACTTGTGCCCTTGGGGCATAAGTTAAGAAGACAAATATAGAAAGTTTTTCTTGAAACTTGTGTATTCAATGTCTAAAACGTTGCAAAAGTTATACTTTCAATGTAAAATTTATTTTTAATTTCTATTTTAATCTCCTTAACTTGTGCCCTTAGGGCACAAGTTAACATGACCCATAAATAAATAATATTTACTGATTAAGTAATTAATCAATGTTGCAGTTTTGTTGGGGAGAGCATGCATGATGATGGAAGCCTTGTGTTTGCTTACTACAAGGATGGTGCCACTGATCCAACTTTTCTCTACTTTGCCTATGGCTTGAAGGAAATCAAGTGCTAA

Protein sequence

>MS.gene056064.t1

MLVYQDLLTGDELLSDSFPYKEIENGMLWEVEGKWVVQGAVDVNIGANPSAEGGDEDDGVDDQAVKVVDIVDTFRLQEQPAFDKKQFVTYMKRYIKLLTSKLEPEKQELFKKHIEAATKFLLPKLKDLQFFVGESMHDDGSLVFAYYKDGATDPTFLYFAYGLKEIKC