AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene051926


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051926.t1 MTR_7g107400 100.000 44 0 0 1 44 1 44 3.42e-26 96.3
MS.gene051926.t1 MTR_3g095810 97.500 40 1 0 5 44 9 48 2.53e-22 85.9
MS.gene051926.t1 MTR_3g095810 97.500 40 1 0 5 44 9 48 2.54e-22 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene051926.t1 AT3G04770 80.952 42 8 0 3 44 9 50 2.05e-20 80.9
MS.gene051926.t1 AT3G04770 79.070 43 9 0 2 44 8 50 5.62e-20 80.1
MS.gene051926.t1 AT1G72370 76.744 43 10 0 2 44 7 49 4.52e-19 77.4
MS.gene051926.t1 AT1G72370 76.744 43 10 0 2 44 7 49 5.01e-19 77.4

Find 13 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAATTTAGGGTTTGGAAAA+TGG 0.189223 7.1:+5938659 None:intergenic
AAATTGGATAATTTAGGGTT+TGG 0.349997 7.1:+5938652 None:intergenic
CATCGGCGGCAAGCATCATC+TGG 0.408335 7.1:+5938756 None:intergenic
GGCAAGCATCATCTGGATAT+CGG 0.457963 7.1:+5938763 None:intergenic
ATCTTCAAGCGCCGTAACGA+TGG 0.529765 7.1:-5938689 MS.gene051926:CDS
ATGGAATGTTACCATCGTTA+CGG 0.545997 7.1:+5938678 None:intergenic
TTTAGTACCGAGATGGACAT+CGG 0.561895 7.1:+5938739 None:intergenic
AAGTTGACGAGGGGCAGCCA+TGG 0.573667 7.1:+5938799 None:intergenic
CTTTCTGAGAAAGTTGACGA+GGG 0.588586 7.1:+5938789 None:intergenic
CTTGCCGCCGATGTCCATCT+CGG 0.588724 7.1:-5938746 MS.gene051926:CDS
TCTTTCTGAGAAAGTTGACG+AGG 0.624055 7.1:+5938788 None:intergenic
TTTCTGAGAAAGTTGACGAG+GGG 0.682900 7.1:+5938790 None:intergenic
AGTACCGAGATGGACATCGG+CGG 0.738432 7.1:+5938742 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGGAATGTTACCATCGTTA+CGG + chr7.1:5938786-5938805 None:intergenic 35.0%
TAAAAACTGCGACTTTCAGA+TGG - chr7.1:5938741-5938760 MS.gene051926:CDS 35.0%
! CTTTCTGAGAAAGTTGACGA+GGG + chr7.1:5938675-5938694 None:intergenic 40.0%
! TCTTTCTGAGAAAGTTGACG+AGG + chr7.1:5938676-5938695 None:intergenic 40.0%
! TTTAGTACCGAGATGGACAT+CGG + chr7.1:5938725-5938744 None:intergenic 40.0%
! TTTCTGAGAAAGTTGACGAG+GGG + chr7.1:5938674-5938693 None:intergenic 40.0%
GGCAAGCATCATCTGGATAT+CGG + chr7.1:5938701-5938720 None:intergenic 45.0%
!!! CGCAGTTTTTAGTACCGAGA+TGG + chr7.1:5938732-5938751 None:intergenic 45.0%
! ATCTTCAAGCGCCGTAACGA+TGG - chr7.1:5938772-5938791 MS.gene051926:CDS 50.0%
! AGTACCGAGATGGACATCGG+CGG + chr7.1:5938722-5938741 None:intergenic 55.0%
CATCGGCGGCAAGCATCATC+TGG + chr7.1:5938708-5938727 None:intergenic 60.0%
CTTGCCGCCGATGTCCATCT+CGG - chr7.1:5938715-5938734 MS.gene051926:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 5938664 5938819 5938664 ID=MS.gene051926
chr7.1 mRNA 5938664 5938819 5938664 ID=MS.gene051926.t1;Parent=MS.gene051926
chr7.1 exon 5938664 5938819 5938664 ID=MS.gene051926.t1.exon1;Parent=MS.gene051926.t1
chr7.1 CDS 5938664 5938819 5938664 ID=cds.MS.gene051926.t1;Parent=MS.gene051926.t1
Gene Sequence

>MS.gene051926

ATGGCTGCCCCTCGTCAACTTTCTCAGAAAGAAGCCGATATCCAGATGATGCTTGCCGCCGATGTCCATCTCGGTACTAAAAACTGCGACTTTCAGATGGAAAGATACATCTTCAAGCGCCGTAACGATGGTAACATTCCATTTTCCAAACCCTAA

Protein sequence

>MS.gene051926.t1

MAAPRQLSQKEADIQMMLAADVHLGTKNCDFQMERYIFKRRNDGNIPFSKP