AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene44316


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44316.t1 MTR_2g087350 94.253 87 5 0 52 138 296 382 3.59e-49 162
MS.gene44316.t1 MTR_1g056880 68.675 83 26 0 56 138 331 413 9.53e-31 114
MS.gene44316.t1 MTR_7g106340 67.470 83 27 0 56 138 315 397 1.15e-30 114
MS.gene44316.t1 MTR_1g056870 66.265 83 28 0 56 138 331 413 1.48e-28 108
MS.gene44316.t1 MTR_2g019860 87.500 56 7 0 2 57 93 148 2.42e-28 109
MS.gene44316.t1 MTR_1g056840 68.116 69 22 0 70 138 319 387 1.82e-22 91.7
MS.gene44316.t1 MTR_4g119100 73.214 56 15 0 2 57 101 156 5.04e-22 91.3
MS.gene44316.t1 MTR_4g119100 73.214 56 15 0 2 57 101 156 5.67e-22 91.3
MS.gene44316.t1 MTR_4g119100 73.214 56 15 0 2 57 100 155 7.15e-22 90.9
MS.gene44316.t1 MTR_4g051515 43.373 83 47 0 56 138 309 391 1.04e-18 81.3
MS.gene44316.t1 MTR_1g056910 39.759 83 50 0 56 138 304 386 7.80e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene44316.t1 AT2G35930 63.855 83 30 0 56 138 307 389 3.40e-29 110
MS.gene44316.t1 AT3G52450 61.446 83 32 0 56 138 331 413 7.46e-28 107
MS.gene44316.t1 AT3G11840 48.193 83 43 0 56 138 344 426 2.11e-20 86.3
MS.gene44316.t1 AT5G18590 67.857 56 18 0 2 57 83 138 2.41e-19 83.6
MS.gene44316.t1 AT5G18590 67.857 56 18 0 2 57 83 138 2.41e-19 83.6

Find 24 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTATGAGTTCTATTTGT+AGG 0.259477 5.4:-23475337 MS.gene44316:CDS
ACTCAAACATGTAAATGATA+TGG 0.362904 5.4:-23475198 MS.gene44316:CDS
AAACTATGACCCTTGCAAAC+TGG 0.372760 5.4:+23476621 None:intergenic
ATAGTGTTTGGTGGTGAATC+TGG 0.382024 5.4:-23476891 MS.gene44316:CDS
AGGAGTTTCAAAGTTGTGTT+TGG 0.433429 5.4:-23475270 MS.gene44316:CDS
ATCTGTAACGGCAGACTACT+TGG 0.440551 5.4:+23476645 None:intergenic
TCTCATGTTGCAAGTGATAG+AGG 0.444489 5.4:-23475365 MS.gene44316:CDS
GTTAAATCATGGAGCAGGAT+TGG 0.461494 5.4:-23475417 MS.gene44316:CDS
GAGAATAAGATGATAGTGTT+TGG 0.481251 5.4:-23476903 MS.gene44316:CDS
AGAGCAGAGTTGTTAAATCA+TGG 0.490916 5.4:-23475428 MS.gene44316:CDS
TTTGGTGGTGAATCTGGAAA+TGG 0.492857 5.4:-23476885 MS.gene44316:CDS
CATGAAATGTTGCGTGTTGG+AGG 0.510565 5.4:-23475290 MS.gene44316:CDS
GAGTTGTTAAATCATGGAGC+AGG 0.539941 5.4:-23475422 MS.gene44316:CDS
GTTACAGATTCCAGTTTGCA+AGG 0.549468 5.4:-23476631 MS.gene44316:CDS
TGGATTGCTAGATGAGATGC+AGG 0.556131 5.4:-23476865 MS.gene44316:intron
CAAGACAAAAGAGAGGGCTA+AGG 0.587076 5.4:-23475225 MS.gene44316:CDS
TTACAGATTCCAGTTTGCAA+GGG 0.593058 5.4:-23476630 MS.gene44316:intron
TTACATGAAATGTTGCGTGT+TGG 0.602505 5.4:-23475293 MS.gene44316:CDS
CATCTTATTCTCAATGACAC+AGG 0.607094 5.4:+23476914 None:intergenic
TTGCAAACTGGAATCTGTAA+CGG 0.615964 5.4:+23476633 None:intergenic
AATAAGATGATAGTGTTTGG+TGG 0.637222 5.4:-23476900 MS.gene44316:CDS
GTAATTTCAAGACAAAAGAG+AGG 0.637702 5.4:-23475232 MS.gene44316:CDS
TAATTTCAAGACAAAAGAGA+GGG 0.648697 5.4:-23475231 MS.gene44316:CDS
GTATAGCTTAGATGACACAG+CGG 0.844638 5.4:+23476674 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTAATTATAATGCAAA+TGG - chr5.4:23475987-23476006 MS.gene44316:intron 10.0%
!! TAATTAATTATAATGCAAAT+GGG - chr5.4:23475988-23476007 MS.gene44316:intron 10.0%
!!! ATTAATATTGACTAATTATT+TGG - chr5.4:23475519-23475538 MS.gene44316:intron 10.0%
!! AAAAAAAAATCCTTTAATCT+GGG + chr5.4:23476166-23476185 None:intergenic 15.0%
!!! AAAATACGTTATTTTTAAGA+AGG + chr5.4:23476134-23476153 None:intergenic 15.0%
!!! TAAATTTTGACAAATTTTCA+TGG - chr5.4:23475592-23475611 MS.gene44316:intron 15.0%
!! GAAAAAAAAATCCTTTAATC+TGG + chr5.4:23476167-23476186 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCCAAAT+AGG - chr5.4:23475260-23475279 MS.gene44316:CDS 20.0%
!!! CAACATACTATTCATTTTAT+TGG - chr5.4:23475562-23475581 MS.gene44316:intron 20.0%
!!! TTAAGTTGTTTATCCAAATA+GGG - chr5.4:23475261-23475280 MS.gene44316:CDS 20.0%
!!! TTCGTTTATTCTTTTTTACT+GGG - chr5.4:23476639-23476658 MS.gene44316:CDS 20.0%
!!! TTTCGTTTATTCTTTTTTAC+TGG - chr5.4:23476638-23476657 MS.gene44316:CDS 20.0%
! AAAATAAGAAAATAGTGACC+AGG - chr5.4:23476395-23476414 MS.gene44316:intron 25.0%
! AAACTATCATTCATCAAGTT+AGG + chr5.4:23476614-23476633 None:intergenic 25.0%
! AAATGAATAGTATGTTGATG+TGG + chr5.4:23475559-23475578 None:intergenic 25.0%
! AACTGACAATAAGTTTAACA+AGG - chr5.4:23475904-23475923 MS.gene44316:intron 25.0%
! ACTCAAACATGTAAATGATA+TGG - chr5.4:23477092-23477111 MS.gene44316:intron 25.0%
! ACTTAAGTCCATTCAAAAAT+TGG + chr5.4:23476530-23476549 None:intergenic 25.0%
! ATGTAAAAGCTAAAGTCATT+AGG - chr5.4:23475195-23475214 MS.gene44316:CDS 25.0%
! ATTCAAATTCTCAAAACTTC+AGG + chr5.4:23476323-23476342 None:intergenic 25.0%
! ATTCATCAAGTTAGGATTAT+TGG + chr5.4:23476606-23476625 None:intergenic 25.0%
! GAATTATGAGTTCTATTTGT+AGG - chr5.4:23476953-23476972 MS.gene44316:intron 25.0%
! GATCAATGCAGATTATAATA+TGG + chr5.4:23476101-23476120 None:intergenic 25.0%
! TAATTTCAAGACAAAAGAGA+GGG - chr5.4:23477059-23477078 MS.gene44316:intron 25.0%
! TGGATAAACAACTTAATGAA+AGG + chr5.4:23475257-23475276 None:intergenic 25.0%
! TTGACTAATTATTTGGTTGT+TGG - chr5.4:23475526-23475545 MS.gene44316:intron 25.0%
!! ATACTATTCATTTTATTGGC+AGG - chr5.4:23475566-23475585 MS.gene44316:intron 25.0%
!! TGTGTCAAAGAAAATTTTGA+GGG - chr5.4:23476900-23476919 MS.gene44316:CDS 25.0%
!! TTGTGTCAAAGAAAATTTTG+AGG - chr5.4:23476899-23476918 MS.gene44316:CDS 25.0%
!!! TATTCTTTTTTACTGGGAAT+CGG - chr5.4:23476645-23476664 MS.gene44316:CDS 25.0%
AAGCTAAAGTCATTAGGTTA+TGG - chr5.4:23475201-23475220 MS.gene44316:CDS 30.0%
ACTAATTATTTGGTTGTTGG+TGG - chr5.4:23475529-23475548 MS.gene44316:intron 30.0%
AGTTGTGAATGAAATTCGAT+TGG + chr5.4:23475888-23475907 None:intergenic 30.0%
ATGTCATTATTAAGGTCACT+TGG + chr5.4:23475757-23475776 None:intergenic 30.0%
GTAATTTCAAGACAAAAGAG+AGG - chr5.4:23477058-23477077 MS.gene44316:intron 30.0%
GTTTGTGAATTGATGTTGAT+AGG - chr5.4:23476808-23476827 MS.gene44316:intron 30.0%
! AATAAGATGATAGTGTTTGG+TGG - chr5.4:23475390-23475409 MS.gene44316:CDS 30.0%
! AGAGCATCATGTCATTATTA+AGG + chr5.4:23475765-23475784 None:intergenic 30.0%
! CAAACTTCTTTTCGATGTTT+CGG - chr5.4:23476777-23476796 MS.gene44316:intron 30.0%
! GAGAATAAGATGATAGTGTT+TGG - chr5.4:23475387-23475406 MS.gene44316:CDS 30.0%
! TACATTTTCCTAGGAAAACT+AGG - chr5.4:23476424-23476443 MS.gene44316:intron 30.0%
! TGAATTGATGTTGATAGGTT+TGG - chr5.4:23476813-23476832 MS.gene44316:intron 30.0%
!! TATTGTTAAGTACTGACTAC+TGG - chr5.4:23475326-23475345 MS.gene44316:CDS 30.0%
AATCCACCCTTAGTAAATTG+AGG - chr5.4:23475832-23475851 MS.gene44316:intron 35.0%
AATTATAATGCAAATGGGCG+TGG - chr5.4:23475993-23476012 MS.gene44316:intron 35.0%
ACTTGATTCATGTAGATGGT+TGG - chr5.4:23476240-23476259 MS.gene44316:intron 35.0%
AGAGCAGAGTTGTTAAATCA+TGG - chr5.4:23476862-23476881 MS.gene44316:intron 35.0%
AGGAGTTTCAAAGTTGTGTT+TGG - chr5.4:23477020-23477039 MS.gene44316:intron 35.0%
AGTCCATTCAAAAATTGGTC+AGG + chr5.4:23476525-23476544 None:intergenic 35.0%
ATAGGTTTGGATCAACTTTG+TGG - chr5.4:23476826-23476845 MS.gene44316:intron 35.0%
ATGGATGAAGTTACATACCA+TGG + chr5.4:23476082-23476101 None:intergenic 35.0%
CATCACTTGATTCATGTAGA+TGG - chr5.4:23476236-23476255 MS.gene44316:intron 35.0%
CATCTTATTCTCAATGACAC+AGG + chr5.4:23475379-23475398 None:intergenic 35.0%
CTTGCTTTCATTCTTGGAAT+AGG - chr5.4:23476030-23476049 MS.gene44316:intron 35.0%
GGACTAACATAAGGATACAT+TGG + chr5.4:23476753-23476772 None:intergenic 35.0%
GTCCATTCAAAAATTGGTCA+GGG + chr5.4:23476524-23476543 None:intergenic 35.0%
GTTTGCTTAGGACTAACATA+AGG + chr5.4:23476762-23476781 None:intergenic 35.0%
TAGCAGCCTCAATTTACTAA+GGG + chr5.4:23475841-23475860 None:intergenic 35.0%
TAGGAAAATGTACAAAGACC+TGG + chr5.4:23476416-23476435 None:intergenic 35.0%
TCCATTCAAAAATTGGTCAG+GGG + chr5.4:23476523-23476542 None:intergenic 35.0%
TCCTAGGAAAACTAGGTAAA+AGG - chr5.4:23476431-23476450 MS.gene44316:intron 35.0%
TCTTGAATGACATGCAGAAT+TGG - chr5.4:23476461-23476480 MS.gene44316:intron 35.0%
TTACAGATTCCAGTTTGCAA+GGG - chr5.4:23475660-23475679 MS.gene44316:intron 35.0%
TTACATGAAATGTTGCGTGT+TGG - chr5.4:23476997-23477016 MS.gene44316:intron 35.0%
TTCGAAAAGCAATTAGTGAG+CGG + chr5.4:23475940-23475959 None:intergenic 35.0%
TTGCAAACTGGAATCTGTAA+CGG + chr5.4:23475660-23475679 None:intergenic 35.0%
TTTCGATGTTTCGGAAAGAA+GGG - chr5.4:23476786-23476805 MS.gene44316:intron 35.0%
! ACTACTGGTCTGTTATTCAT+TGG - chr5.4:23475341-23475360 MS.gene44316:CDS 35.0%
! AGGTCTTTGTACATTTTCCT+AGG - chr5.4:23476415-23476434 MS.gene44316:intron 35.0%
! CATCGAAAAGAAGTTTGCTT+AGG + chr5.4:23476774-23476793 None:intergenic 35.0%
! CTTGGAATAGGATTGCATTT+TGG - chr5.4:23476042-23476061 MS.gene44316:intron 35.0%
! TTTTCGATGTTTCGGAAAGA+AGG - chr5.4:23476785-23476804 MS.gene44316:intron 35.0%
!! ACCTTTTACCTAGTTTTCCT+AGG + chr5.4:23476435-23476454 None:intergenic 35.0%
!! CTTAAGTTGAAGTTCATTGC+AGG - chr5.4:23476543-23476562 MS.gene44316:intron 35.0%
!!! AACATTTCTGGTTTTGGACT+TGG - chr5.4:23475458-23475477 MS.gene44316:intron 35.0%
AAACTATGACCCTTGCAAAC+TGG + chr5.4:23475672-23475691 None:intergenic 40.0%
AACTTTGTGGTTGTGCAGAA+GGG - chr5.4:23476839-23476858 MS.gene44316:intron 40.0%
AGTTTGCAAGGGTCATAGTT+TGG - chr5.4:23475671-23475690 MS.gene44316:intron 40.0%
CATATGACGAAGACCCTATT+TGG + chr5.4:23475277-23475296 None:intergenic 40.0%
GAAGTTACATACCATGGAGA+AGG + chr5.4:23476076-23476095 None:intergenic 40.0%
GAGTTGTTAAATCATGGAGC+AGG - chr5.4:23476868-23476887 MS.gene44316:CDS 40.0%
GTAGCAGCCTCAATTTACTA+AGG + chr5.4:23475842-23475861 None:intergenic 40.0%
GTATAGCTTAGATGACACAG+CGG + chr5.4:23475619-23475638 None:intergenic 40.0%
GTTAAATCATGGAGCAGGAT+TGG - chr5.4:23476873-23476892 MS.gene44316:CDS 40.0%
GTTACAGATTCCAGTTTGCA+AGG - chr5.4:23475659-23475678 MS.gene44316:intron 40.0%
TCTCATGTTGCAAGTGATAG+AGG - chr5.4:23476925-23476944 MS.gene44316:intron 40.0%
TGATGCTCTCATCATAGACA+TGG - chr5.4:23475776-23475795 MS.gene44316:intron 40.0%
TGGATATTCATCCTTCTCCA+TGG - chr5.4:23476062-23476081 MS.gene44316:intron 40.0%
TGTTCAGATGCCCAGATTAA+AGG - chr5.4:23476153-23476172 MS.gene44316:intron 40.0%
TTGGCACTTGCTTTCATTCT+TGG - chr5.4:23476024-23476043 MS.gene44316:intron 40.0%
! ACCCCTGACCAATTTTTGAA+TGG - chr5.4:23476519-23476538 MS.gene44316:intron 40.0%
!! ATAGTGTTTGGTGGTGAATC+TGG - chr5.4:23475399-23475418 MS.gene44316:CDS 40.0%
!! TTTGGTGGTGAATCTGGAAA+TGG - chr5.4:23475405-23475424 MS.gene44316:CDS 40.0%
!!! TGAGGGAACATTTCTGGTTT+TGG - chr5.4:23475452-23475471 MS.gene44316:intron 40.0%
ATCTGTAACGGCAGACTACT+TGG + chr5.4:23475648-23475667 None:intergenic 45.0%
CAACTTTGTGGTTGTGCAGA+AGG - chr5.4:23476838-23476857 MS.gene44316:intron 45.0%
CAAGACAAAAGAGAGGGCTA+AGG - chr5.4:23477065-23477084 MS.gene44316:intron 45.0%
CAGCCTCAATTTACTAAGGG+TGG + chr5.4:23475838-23475857 None:intergenic 45.0%
CATGAAATGTTGCGTGTTGG+AGG - chr5.4:23477000-23477019 MS.gene44316:intron 45.0%
GGACTGTCACAGACTTTCAT+AGG - chr5.4:23475222-23475241 MS.gene44316:CDS 45.0%
TGGATTGCTAGATGAGATGC+AGG - chr5.4:23475425-23475444 MS.gene44316:CDS 45.0%
AATGGGCGTGGTTCTTGGAT+TGG - chr5.4:23476005-23476024 MS.gene44316:intron 50.0%
AGATGAGATGCAGGTGAGTG+AGG - chr5.4:23475434-23475453 MS.gene44316:CDS 50.0%
ATGCAAATGGGCGTGGTTCT+TGG - chr5.4:23476000-23476019 MS.gene44316:intron 50.0%
GATGAGATGCAGGTGAGTGA+GGG - chr5.4:23475435-23475454 MS.gene44316:CDS 50.0%
GGTGAGTGAGGGAACATTTC+TGG - chr5.4:23475446-23475465 MS.gene44316:intron 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 23475190 23477122 23475190 ID=MS.gene44316
chr5.4 mRNA 23475190 23477122 23475190 ID=MS.gene44316.t1;Parent=MS.gene44316
chr5.4 exon 23477116 23477122 23477116 ID=MS.gene44316.t1.exon1;Parent=MS.gene44316.t1
chr5.4 CDS 23477116 23477122 23477116 ID=cds.MS.gene44316.t1;Parent=MS.gene44316.t1
chr5.4 exon 23476866 23476936 23476866 ID=MS.gene44316.t1.exon2;Parent=MS.gene44316.t1
chr5.4 CDS 23476866 23476936 23476866 ID=cds.MS.gene44316.t1;Parent=MS.gene44316.t1
chr5.4 exon 23476631 23476724 23476631 ID=MS.gene44316.t1.exon3;Parent=MS.gene44316.t1
chr5.4 CDS 23476631 23476724 23476631 ID=cds.MS.gene44316.t1;Parent=MS.gene44316.t1
chr5.4 exon 23475190 23475455 23475190 ID=MS.gene44316.t1.exon4;Parent=MS.gene44316.t1
chr5.4 CDS 23475190 23475455 23475190 ID=cds.MS.gene44316.t1;Parent=MS.gene44316.t1
Gene Sequence

>MS.gene44316

ATGGCATGTAAAAGCTAAAGTCATTAGGTTATGGACTGTCACAGACTTTCATAGGCAAATTGTACCTTTCATTAAGTTGTTTATCCAAATAGGGTCTTCGTCATATGTCATAATTTATAGAAATGTTATGAGTTGTTATTGTTAAGTACTGACTACTGGTCTGTTATTCATTGGAATAACATGCAGCCTGTGTCATTGAGAATAAGATGATAGTGTTTGGTGGTGAATCTGGAAATGGATTGCTAGATGAGATGCAGGTGAGTGAGGGAACATTTCTGGTTTTGGACTTGGAAAATTTATGATAGAGAGTTTGTTGTTACTATATGAGCATTAATATTGACTAATTATTTGGTTGTTGGTGGTTAACCACATCAACATACTATTCATTTTATTGGCAGGTTCTAAATTTTGACAAATTTTCATGGACCGCTGTGTCATCTAAGCTATACTTGTCACCAAGTAGTCTGCCGTTACAGATTCCAGTTTGCAAGGGTCATAGTTTGGTATATAAATATATGTTTTTGTCAATAATTGTTATCATTCAGACATGTTTAATTAATTTTTCCAAGTGACCTTAATAATGACATGATGCTCTCATCATAGACATGGAATAACATGTCTGTTTAGATGAGAAGTTCATGTAATCCACCCTTAGTAAATTGAGGCTGCTACAAATGAGATGCTTACAAATGCAACCAATCGAATTTCATTCACAACTGACAATAAGTTTAACAAGGACTATAAACTCCGCTCACTAATTGCTTTTCGAATAATACATTGAGTTGTAAATGAGATAGATAATTAATTATAATGCAAATGGGCGTGGTTCTTGGATTGGCACTTGCTTTCATTCTTGGAATAGGATTGCATTTTGGATATTCATCCTTCTCCATGGTATGTAACTTCATCCATATTATAATCTGCATTGATCTTTTATTTAGCCTTCTTAAAAATAACGTATTTTGTTCAGATGCCCAGATTAAAGGATTTTTTTTTCAAACATAACTTAATTCAAGTGTATTTTATACTTCTTAAACATTAAATGACATCACTTGATTCATGTAGATGGTTGGACGTAATAAAAAACATTGACTGTTGTTTTGTGTCGCTGCTGCTATATACAAGTTATTCCTGAAGTTTTGAGAATTTGAATTATGAGTTCAACACTTAATGTATTTGCTTCTGTTAGCTTGATTGTACTGTTTAAAATAAGAAAATAGTGACCAGGTCTTTGTACATTTTCCTAGGAAAACTAGGTAAAAGGTTTTGATTCTTGAATGACATGCAGAATTGGCATTTTGCATTGTCTTAATTATGACATTGAATATAACCCCTGACCAATTTTTGAATGGACTTAAGTTGAAGTTCATTGCAGGATAATATTGCTAAATATATCTTTCTTTTATTGTTTAACCAATAATCCTAACTTGATGAATGATAGTTTAGTTTTTCGTTTATTCTTTTTTACTGGGAATCGGTTTTTCTGTATTTGTTCATATCTAATGTCAGCAAGCTTTAGTAATATTTCAATCTAAATATTATGTAGTTGTAATGTCTCTACCAATGTATCCTTATGTTAGTCCTAAGCAAACTTCTTTTCGATGTTTCGGAAAGAAGGGTTTGTGAATTGATGTTGATAGGTTTGGATCAACTTTGTGGTTGTGCAGAAGGGAGAGCAGAGTTGTTAAATCATGGAGCAGGATTGGCTATTGTGTCAAAGAAAATTTTGAGGGTTTCTCATGTTGCAAGTGATAGAGGTGTTAGAATTATGAGTTCTATTTGTAGGTATTCTGCAAATTCTAGAGTTTTACATGAAATGTTGCGTGTTGGAGGAGTTTCAAAGTTGTGTTTGGTGCTTCAAGTTGATAGTAATTTCAAGACAAAAGAGAGGGCTAAGGAGATACTCAAACATGTAAATGATATGGCTAACTAA

Protein sequence

>MS.gene44316.t1

MASCVIENKMIVFGGESGNGLLDEMQVLNFDKFSWTAVSSKLYLSPSSLPLQIPVCKEGRAELLNHGAGLAIVSKKILRVSHVASDRGVRIMSSICRYSANSRVLHEMLRVGGVSKLCLVLQVDSNFKTKERAKEILKHVNDMAN