AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053966


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTCTACATATGGATTCT+TGG 0.262711 5.2:-42965401 MS.gene053966:CDS
AAAAGGACAAAGAGCATCTA+AGG 0.339255 5.2:-42965507 MS.gene053966:CDS
TGAATTGATGTCTCTAGAAA+AGG 0.480662 5.2:-42965524 MS.gene053966:CDS
AAAGACGAGTCTTCTACATA+TGG 0.492701 5.2:-42965409 MS.gene053966:CDS
AGCTGCTGTCAATGTGGAGC+GGG 0.505097 5.2:-42965348 None:intergenic
TAAGCTCGAAAGAGCTGAAG+AGG 0.554165 5.2:-42965482 MS.gene053966:CDS
TGACCTCCTTGACTCTCTTG+TGG 0.562654 5.2:-42965446 MS.gene053966:CDS
GCTCGAAAGAGCTGAAGAGG+AGG 0.578578 5.2:-42965479 MS.gene053966:CDS
AAAGAGCTGAAGAGGAGGTG+AGG 0.606699 5.2:-42965474 MS.gene053966:CDS
GTAGCTTAAAACAATCAACT+CGG 0.647486 5.2:-42965567 None:intergenic
AAGCTGCTGTCAATGTGGAG+CGG 0.655453 5.2:-42965349 MS.gene053966:CDS
CTGCTGCCACAAGAGAGTCA+AGG 0.705247 5.2:+42965440 None:intergenic
CTGCCACAAGAGAGTCAAGG+AGG 0.721597 5.2:+42965443 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGAATTGATGTCTCTAGAAA+AGG - chr5.2:42965388-42965407 MS.gene053966:CDS 30.0%
AAAGACGAGTCTTCTACATA+TGG - chr5.2:42965503-42965522 MS.gene053966:CDS 35.0%
GTCTTCTACATATGGATTCT+TGG - chr5.2:42965511-42965530 MS.gene053966:CDS 35.0%
!! AAAAGGACAAAGAGCATCTA+AGG - chr5.2:42965405-42965424 MS.gene053966:CDS 35.0%
GTAAAAAGCTGCTGTCAATG+TGG - chr5.2:42965558-42965577 MS.gene053966:CDS 40.0%
TAAGCTCGAAAGAGCTGAAG+AGG - chr5.2:42965430-42965449 MS.gene053966:CDS 45.0%
AAAGAGCTGAAGAGGAGGTG+AGG - chr5.2:42965438-42965457 MS.gene053966:CDS 50.0%
AAGCTGCTGTCAATGTGGAG+CGG - chr5.2:42965563-42965582 MS.gene053966:CDS 50.0%
TGACCTCCTTGACTCTCTTG+TGG - chr5.2:42965466-42965485 MS.gene053966:CDS 50.0%
CTGCCACAAGAGAGTCAAGG+AGG + chr5.2:42965472-42965491 None:intergenic 55.0%
CTGCTGCCACAAGAGAGTCA+AGG + chr5.2:42965475-42965494 None:intergenic 55.0%
GCTCGAAAGAGCTGAAGAGG+AGG - chr5.2:42965433-42965452 MS.gene053966:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 42965349 42965585 42965349 ID=MS.gene053966
chr5.2 mRNA 42965349 42965585 42965349 ID=MS.gene053966.t1;Parent=MS.gene053966
chr5.2 exon 42965349 42965585 42965349 ID=MS.gene053966.t1.exon1;Parent=MS.gene053966.t1
chr5.2 CDS 42965349 42965585 42965349 ID=cds.MS.gene053966.t1;Parent=MS.gene053966.t1
Gene Sequence

>MS.gene053966

CTTAAAACAATCAACTCGGAGAGTATTGTCTCTCGTTGCTGAATTGATGTCTCTAGAAAAGGACAAAGAGCATCTAAGGATTAAGCTCGAAAGAGCTGAAGAGGAGGTGAGGATTGCTGACCTCCTTGACTCTCTTGTGGCAGCAGTTATTACTAAAGACGAGTCTTCTACATATGGATTCTTGGTTGCAAATAGTGAAAATGAGCATTGTAAAAAGCTGCTGTCAATGTGGAGCGG

Protein sequence

>MS.gene053966.t1

LKTINSESIVSRCUIDVSRKGQRASKDUARKSURGGEDCUPPULSCGSSYYURRVFYIWILGCKUUKUALUKAAVNVER