Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44375.t1 | XP_003613224.1 | 98.1 | 108 | 2 | 0 | 61 | 168 | 61 | 168 | 1.50E-42 | 182.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44375.t1 | G7K1C9 | 98.1 | 108 | 2 | 0 | 61 | 168 | 61 | 168 | 1.1e-42 | 182.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene44375 | 0.879122 | 1.61E-69 | -1.69E-46 |
| MS.gene050297 | MS.gene44375 | 0.80755 | 4.79E-50 | -1.69E-46 |
| MS.gene051067 | MS.gene44375 | 0.812172 | 4.85E-51 | -1.69E-46 |
| MS.gene051478 | MS.gene44375 | 0.845123 | 4.94E-59 | -1.69E-46 |
| MS.gene053144 | MS.gene44375 | 0.817494 | 3.20E-52 | -1.69E-46 |
| MS.gene053536 | MS.gene44375 | 0.821723 | 3.47E-53 | -1.69E-46 |
| MS.gene053538 | MS.gene44375 | 0.822729 | 2.03E-53 | -1.69E-46 |
| MS.gene054118 | MS.gene44375 | 0.827371 | 1.62E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene44375 | 0.810462 | 1.14E-50 | -1.69E-46 |
| MS.gene054999 | MS.gene44375 | 0.803756 | 2.99E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene44375 | 0.819839 | 9.40E-53 | -1.69E-46 |
| MS.gene055764 | MS.gene44375 | 0.804311 | 2.30E-49 | -1.69E-46 |
| MS.gene056704 | MS.gene44375 | 0.803222 | 3.86E-49 | -1.69E-46 |
| MS.gene057082 | MS.gene44375 | 0.82136 | 4.21E-53 | -1.69E-46 |
| MS.gene05866 | MS.gene44375 | 0.80367 | 3.12E-49 | -1.69E-46 |
| MS.gene058909 | MS.gene44375 | 0.820875 | 5.44E-53 | -1.69E-46 |
| MS.gene058912 | MS.gene44375 | 0.820963 | 5.20E-53 | -1.69E-46 |
| MS.gene05894 | MS.gene44375 | 0.811356 | 7.29E-51 | -1.69E-46 |
| MS.gene059300 | MS.gene44375 | 0.804513 | 2.08E-49 | -1.69E-46 |
| MS.gene059424 | MS.gene44375 | 0.811724 | 6.07E-51 | -1.69E-46 |
| MS.gene06057 | MS.gene44375 | 0.800305 | 1.53E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44375.t1 | MTR_5g034200 | 99.405 | 168 | 1 | 0 | 1 | 168 | 1 | 168 | 4.74e-122 | 341 |
| MS.gene44375.t1 | MTR_8g064760 | 38.608 | 158 | 73 | 6 | 20 | 167 | 18 | 161 | 5.43e-21 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44375.t1 | AT1G23530 | 33.690 | 187 | 89 | 8 | 11 | 167 | 7 | 188 | 2.93e-16 | 72.8 |
Find 33 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAAGTTGATATTGAATT+TGG | 0.135097 | 5.1:+25120919 | MS.gene44375:CDS |
| AATTCAATATCAACTTCTTT+TGG | 0.244618 | 5.1:-25120916 | None:intergenic |
| CATTCATCAAATGGATCATT+TGG | 0.283971 | 5.1:+25120745 | MS.gene44375:CDS |
| CTGTTGCTTGTTGTCTTGGA+AGG | 0.386601 | 5.1:+25120809 | MS.gene44375:CDS |
| TCTGCTGTTGCTTGTTGTCT+TGG | 0.387132 | 5.1:+25120805 | MS.gene44375:CDS |
| CCTCCATGTCCATTGTTCAT+AGG | 0.387145 | 5.1:-25120970 | None:intergenic |
| GATGAATGGTGGTCAGGTTC+AGG | 0.392297 | 5.1:-25120730 | None:intergenic |
| TTTCAAAACTCTTCATATCA+TGG | 0.397060 | 5.1:-25121080 | None:intergenic |
| TCATGGTGACCTTGAGCAAC+TGG | 0.407489 | 5.1:-25121063 | None:intergenic |
| TGTTGTTGTTGTTGCTGCAT+TGG | 0.421046 | 5.1:-25120658 | None:intergenic |
| ATTCATCAAATGGATCATTT+GGG | 0.438895 | 5.1:+25120746 | MS.gene44375:CDS |
| AGTATAGGCCAGGTGCATAA+TGG | 0.443427 | 5.1:+25121127 | MS.gene44375:CDS |
| CCTGACCACCATTCATCAAA+TGG | 0.451998 | 5.1:+25120736 | MS.gene44375:CDS |
| CAGGTTCAGGTGGTGCATAC+TGG | 0.460578 | 5.1:-25120717 | None:intergenic |
| CCATTTGATGAATGGTGGTC+AGG | 0.470979 | 5.1:-25120736 | None:intergenic |
| GTGGTTGGATAAACTTGAAC+TGG | 0.498993 | 5.1:-25120685 | None:intergenic |
| AGCAGAAATTACTATTATGA+TGG | 0.518824 | 5.1:-25120788 | None:intergenic |
| GTCCTCCTCAAGGAAAGTAT+AGG | 0.536696 | 5.1:+25121112 | MS.gene44375:CDS |
| CAAATGATCCATTTGATGAA+TGG | 0.542377 | 5.1:-25120744 | None:intergenic |
| TACTGGTATGTGACAGTGGT+TGG | 0.552488 | 5.1:-25120700 | None:intergenic |
| GCTTAACAGGTTTCTGACTG+TGG | 0.553296 | 5.1:-25120861 | None:intergenic |
| ATAAAGCTTGGTCCTCCTCA+AGG | 0.553613 | 5.1:+25121102 | MS.gene44375:CDS |
| TTGTAACTCCATTATGCACC+TGG | 0.558632 | 5.1:-25121135 | None:intergenic |
| CCTCAAGGAAAGTATAGGCC+AGG | 0.560026 | 5.1:+25121117 | MS.gene44375:CDS |
| GGCCTATACTTTCCTTGAGG+AGG | 0.568497 | 5.1:-25121114 | None:intergenic |
| CCTGGCCTATACTTTCCTTG+AGG | 0.589930 | 5.1:-25121117 | None:intergenic |
| GTACAGAAGCCAGTTGCTCA+AGG | 0.601141 | 5.1:+25121054 | MS.gene44375:CDS |
| GAATGGTGGTCAGGTTCAGG+TGG | 0.612964 | 5.1:-25120727 | None:intergenic |
| GCTTCTAAGCCTATGAACAA+TGG | 0.625263 | 5.1:+25120961 | MS.gene44375:CDS |
| AAGCCTATGAACAATGGACA+TGG | 0.633051 | 5.1:+25120967 | MS.gene44375:CDS |
| TGCATACTGGTATGTGACAG+TGG | 0.646713 | 5.1:-25120704 | None:intergenic |
| ATGATCCATTTGATGAATGG+TGG | 0.679103 | 5.1:-25120741 | None:intergenic |
| CCTATGAACAATGGACATGG+AGG | 0.679752 | 5.1:+25120970 | MS.gene44375:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAAGTTGATATTGAATT+TGG | + | chr5.1:25120919-25120938 | MS.gene44375:CDS | 20.0% |
| !!! | AATTCAATATCAACTTCTTT+TGG | - | chr5.1:25120919-25120938 | None:intergenic | 20.0% |
| !!! | TTTTCAAAAAATAATGGACA+TGG | + | chr5.1:25120994-25121013 | MS.gene44375:CDS | 20.0% |
| ! | AGCAGAAATTACTATTATGA+TGG | - | chr5.1:25120791-25120810 | None:intergenic | 25.0% |
| ! | ATTCATCAAATGGATCATTT+GGG | + | chr5.1:25120746-25120765 | MS.gene44375:CDS | 25.0% |
| ! | TTTCAAAACTCTTCATATCA+TGG | - | chr5.1:25121083-25121102 | None:intergenic | 25.0% |
| !!! | AAGAGTTTTGAAATAAAGCT+TGG | + | chr5.1:25121090-25121109 | MS.gene44375:CDS | 25.0% |
| CAAATGATCCATTTGATGAA+TGG | - | chr5.1:25120747-25120766 | None:intergenic | 30.0% | |
| CATTCATCAAATGGATCATT+TGG | + | chr5.1:25120745-25120764 | MS.gene44375:CDS | 30.0% | |
| ! | GGAGGCTTTTCAAAAAATAA+TGG | + | chr5.1:25120988-25121007 | MS.gene44375:CDS | 30.0% |
| !! | TCAAAAAATAATGGACATGG+AGG | + | chr5.1:25120997-25121016 | MS.gene44375:CDS | 30.0% |
| ATGATCCATTTGATGAATGG+TGG | - | chr5.1:25120744-25120763 | None:intergenic | 35.0% | |
| AAGCCTATGAACAATGGACA+TGG | + | chr5.1:25120967-25120986 | MS.gene44375:CDS | 40.0% | |
| GCTTCTAAGCCTATGAACAA+TGG | + | chr5.1:25120961-25120980 | MS.gene44375:CDS | 40.0% | |
| GTGGTTGGATAAACTTGAAC+TGG | - | chr5.1:25120688-25120707 | None:intergenic | 40.0% | |
| ! | TGTTGTTGTTGTTGCTGCAT+TGG | - | chr5.1:25120661-25120680 | None:intergenic | 40.0% |
| !!! | TGCTGCTTTTGATGCTTAAC+AGG | - | chr5.1:25120877-25120896 | None:intergenic | 40.0% |
| ATAAAGCTTGGTCCTCCTCA+AGG | + | chr5.1:25121102-25121121 | MS.gene44375:CDS | 45.0% | |
| CCATTTGATGAATGGTGGTC+AGG | - | chr5.1:25120739-25120758 | None:intergenic | 45.0% | |
| CCTATGAACAATGGACATGG+AGG | + | chr5.1:25120970-25120989 | MS.gene44375:CDS | 45.0% | |
| CCTCCATGTCCATTGTTCAT+AGG | - | chr5.1:25120973-25120992 | None:intergenic | 45.0% | |
| CCTGACCACCATTCATCAAA+TGG | + | chr5.1:25120736-25120755 | MS.gene44375:CDS | 45.0% | |
| GCTTAACAGGTTTCTGACTG+TGG | - | chr5.1:25120864-25120883 | None:intergenic | 45.0% | |
| TACTGGTATGTGACAGTGGT+TGG | - | chr5.1:25120703-25120722 | None:intergenic | 45.0% | |
| TGCATACTGGTATGTGACAG+TGG | - | chr5.1:25120707-25120726 | None:intergenic | 45.0% | |
| ! | CTTCTTTTGGACGTGAGTGT+TGG | - | chr5.1:25120906-25120925 | None:intergenic | 45.0% |
| ! | GTCCTCCTCAAGGAAAGTAT+AGG | + | chr5.1:25121112-25121131 | MS.gene44375:CDS | 45.0% |
| !! | CTGTTGCTTGTTGTCTTGGA+AGG | + | chr5.1:25120809-25120828 | MS.gene44375:CDS | 45.0% |
| !! | TCTGCTGTTGCTTGTTGTCT+TGG | + | chr5.1:25120805-25120824 | MS.gene44375:CDS | 45.0% |
| CCTGGCCTATACTTTCCTTG+AGG | - | chr5.1:25121120-25121139 | None:intergenic | 50.0% | |
| GATGAATGGTGGTCAGGTTC+AGG | - | chr5.1:25120733-25120752 | None:intergenic | 50.0% | |
| GGCCTATACTTTCCTTGAGG+AGG | - | chr5.1:25121117-25121136 | None:intergenic | 50.0% | |
| GTACAGAAGCCAGTTGCTCA+AGG | + | chr5.1:25121054-25121073 | MS.gene44375:CDS | 50.0% | |
| TCATGGTGACCTTGAGCAAC+TGG | - | chr5.1:25121066-25121085 | None:intergenic | 50.0% | |
| ! | CCTCAAGGAAAGTATAGGCC+AGG | + | chr5.1:25121117-25121136 | MS.gene44375:CDS | 50.0% |
| CAGGTTCAGGTGGTGCATAC+TGG | - | chr5.1:25120720-25120739 | None:intergenic | 55.0% | |
| GAATGGTGGTCAGGTTCAGG+TGG | - | chr5.1:25120730-25120749 | None:intergenic | 55.0% | |
| ! | TGTGGCTTTTGCCGTTGCCG+CGG | - | chr5.1:25120846-25120865 | None:intergenic | 60.0% |
| ! | GGAAGGTTTTGCAGCCGCCG+CGG | + | chr5.1:25120826-25120845 | MS.gene44375:CDS | 65.0% |
| ! | TTTTGCAGCCGCCGCGGCAA+CGG | + | chr5.1:25120832-25120851 | MS.gene44375:CDS | 65.0% |
| ! | GGCTTTTGCCGTTGCCGCGG+CGG | - | chr5.1:25120843-25120862 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 25120640 | 25121146 | 25120640 | ID=MS.gene44375 |
| chr5.1 | mRNA | 25120640 | 25121146 | 25120640 | ID=MS.gene44375.t1;Parent=MS.gene44375 |
| chr5.1 | exon | 25120640 | 25121146 | 25120640 | ID=MS.gene44375.t1.exon1;Parent=MS.gene44375.t1 |
| chr5.1 | CDS | 25120640 | 25121146 | 25120640 | ID=cds.MS.gene44375.t1;Parent=MS.gene44375.t1 |
| Gene Sequence |
| Protein sequence |
