AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45142


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45142.t1 MTR_5g035980 95.595 454 14 3 1 454 1 448 0.0 878
MS.gene45142.t1 MTR_3g078380 52.315 432 150 14 37 454 25 414 7.08e-136 397
MS.gene45142.t1 MTR_4g106480 50.824 425 162 10 35 454 19 401 4.15e-133 390
MS.gene45142.t1 MTR_8g089650 47.017 419 158 13 45 451 64 430 2.36e-110 333
MS.gene45142.t1 MTR_4g104410 42.184 403 170 15 55 449 16 363 6.33e-82 257
MS.gene45142.t1 MTR_5g018520 34.624 439 227 9 13 450 41 420 4.53e-79 253
MS.gene45142.t1 MTR_1g021590 35.821 402 203 8 48 449 85 431 1.26e-77 249
MS.gene45142.t1 MTR_4g068457 35.731 431 208 12 36 449 78 456 1.57e-71 234
MS.gene45142.t1 MTR_2g060070 34.518 394 194 14 58 449 50 381 2.12e-65 216
MS.gene45142.t1 MTR_3g110185 32.373 451 229 12 18 451 20 411 9.40e-51 184
MS.gene45142.t1 MTR_2g101410 31.514 403 203 15 58 451 71 409 3.38e-44 160
MS.gene45142.t1 MTR_2g086140 33.728 169 99 5 247 412 16 174 3.66e-14 75.5
MS.gene45142.t1 MTR_4g053675 30.994 171 105 5 247 412 16 178 8.91e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45142.t1 AT1G24130 54.746 453 156 10 6 454 8 415 1.01e-140 409
MS.gene45142.t1 AT5G50120 45.652 414 185 9 43 452 10 387 2.12e-117 349
MS.gene45142.t1 AT1G24530 45.278 413 167 12 39 451 52 405 5.39e-103 313
MS.gene45142.t1 AT2G26490 36.883 385 185 9 70 454 97 423 8.01e-75 242
MS.gene45142.t1 AT3G50390 35.222 406 195 11 54 449 77 424 1.15e-71 234
MS.gene45142.t1 AT3G51930 34.783 414 222 11 38 449 27 394 9.21e-70 227
MS.gene45142.t1 AT3G18950 35.279 394 196 10 60 449 126 464 1.04e-68 226
MS.gene45142.t1 AT4G34380 34.615 390 201 9 64 449 117 456 3.41e-67 223
MS.gene45142.t1 AT1G49450 33.941 439 225 11 24 449 80 466 4.15e-67 222
MS.gene45142.t1 AT1G47610 33.760 391 200 13 64 452 16 349 3.12e-61 203
MS.gene45142.t1 AT1G21651 27.368 190 105 6 199 386 522 680 6.17e-12 68.6
MS.gene45142.t1 AT1G21650 27.368 190 105 6 199 386 522 680 6.29e-12 68.6
MS.gene45142.t1 AT3G44530 30.539 167 101 5 247 409 16 171 4.77e-11 65.9
MS.gene45142.t1 AT5G16750 25.681 257 145 12 167 408 361 586 5.06e-11 65.5
MS.gene45142.t1 AT3G44530 30.539 167 101 5 247 409 16 171 5.68e-11 65.5

Find 56 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTGTTGTTTCATCTTC+TGG 0.080562 5.1:+26675047 MS.gene45142:CDS
GGGATAGAACATTCAAAATT+TGG 0.193532 5.1:+26674964 MS.gene45142:CDS
TAGCCTTAGTATGCCCTCTT+AGG 0.234642 5.1:-26675370 None:intergenic
CTGATTATAGTGTTAGATTA+TGG 0.250597 5.1:+26675438 MS.gene45142:CDS
AACAACAATCATTGACTTGA+TGG 0.262801 5.1:-26674670 None:intergenic
CACTCTAAATTTCTCTTTAG+TGG 0.264901 5.1:+26674543 MS.gene45142:CDS
CAATTGTTGTTTCATCTTCT+GGG 0.279540 5.1:+26675048 MS.gene45142:CDS
AAATCTTCTTATTTCACTAT+TGG 0.283165 5.1:-26674571 None:intergenic
CTGATAGAAAGATCAAAATT+TGG 0.302795 5.1:+26675090 MS.gene45142:CDS
TCTTCTGGGGTTGTTTATAC+AGG 0.311488 5.1:+26675062 MS.gene45142:CDS
TCTCTTGCTGTTTCGAAAGA+TGG 0.341375 5.1:+26674918 MS.gene45142:CDS
TACTAAGGCTATATTGTGTT+TGG 0.351474 5.1:+26675382 MS.gene45142:CDS
TGTAAAGCAAGGGATGAGAT+AGG 0.355875 5.1:-26674522 None:intergenic
AGCATTATTCGAAATTGTTC+CGG 0.359690 5.1:+26675302 MS.gene45142:CDS
CTCATCAAGATCACAAAATC+CGG 0.361786 5.1:+26674712 MS.gene45142:CDS
TTGATTGTGATATCAAAGTT+TGG 0.373353 5.1:+26675621 MS.gene45142:CDS
TTTGGTTGTGATGGATGATT+TGG 0.391313 5.1:+26675400 MS.gene45142:CDS
AAGATCACAAAATCCGGGTT+TGG 0.420178 5.1:+26674718 MS.gene45142:CDS
TAAAGCAAGGGATGAGATAG+GGG 0.420234 5.1:-26674520 None:intergenic
TTTGAAGCTTGAAGAGCAAA+AGG 0.427788 5.1:-26674603 None:intergenic
AGACCCAGACGACGAAAATA+TGG 0.436892 5.1:+26675334 MS.gene45142:CDS
AGATGGATTGTTTCTTTACT+CGG 0.453556 5.1:+26674935 MS.gene45142:CDS
AGTGTTAGATTATGGAAAAG+AGG 0.466262 5.1:+26675446 MS.gene45142:CDS
TATATTGTGTTTGGTTGTGA+TGG 0.472582 5.1:+26675391 MS.gene45142:CDS
ATTCTGAATGAATGTGATCA+TGG 0.475540 5.1:-26674380 None:intergenic
GTCTGGGTCTTGGTCTAGTC+CGG 0.475863 5.1:-26675321 None:intergenic
GTAAAGCAAGGGATGAGATA+GGG 0.485394 5.1:-26674521 None:intergenic
GTCCTAGTTGGTGCCCTAAG+AGG 0.490038 5.1:+26675356 MS.gene45142:CDS
TGTTTCTTTACTCGGCTTCG+TGG 0.493077 5.1:+26674943 MS.gene45142:CDS
GAAATTTAGAGTGTAAAGCA+AGG 0.496059 5.1:-26674533 None:intergenic
AAACTTCTAACAACAATCAA+TGG 0.516871 5.1:+26674489 MS.gene45142:CDS
GAAGGAACAGAAGGTAGACT+TGG 0.519410 5.1:-26674432 None:intergenic
AGCCTTAGTATGCCCTCTTA+GGG 0.526558 5.1:-26675369 None:intergenic
GTTTCTTTACTCGGCTTCGT+GGG 0.536613 5.1:+26674944 MS.gene45142:CDS
GCTTTAGCACTAAACAAAGA+TGG 0.541869 5.1:+26675221 MS.gene45142:CDS
GAAGTGAGAGAAGGAACAGA+AGG 0.544583 5.1:-26674441 None:intergenic
ATGGATGATTTGGTGTGTAG+TGG 0.553153 5.1:+26675410 MS.gene45142:CDS
TCCTAGTTGGTGCCCTAAGA+GGG 0.557068 5.1:+26675357 MS.gene45142:CDS
ATAGCAGATGATTCCAAAAG+TGG 0.557521 5.1:+26675539 MS.gene45142:CDS
TTGAAGCTTGAAGAGCAAAA+GGG 0.560630 5.1:-26674602 None:intergenic
AAATTTAGAGTGTAAAGCAA+GGG 0.561546 5.1:-26674532 None:intergenic
TGCCCTAAGAGGGCATACTA+AGG 0.569818 5.1:+26675367 MS.gene45142:CDS
GACGAAAATATGGTCCTAGT+TGG 0.572008 5.1:+26675344 MS.gene45142:CDS
TTCAAACAATATCAACAACT+TGG 0.579730 5.1:+26674620 MS.gene45142:CDS
GCCCTCTTAGGGCACCAACT+AGG 0.579791 5.1:-26675358 None:intergenic
AATTGTTGTTTCATCTTCTG+GGG 0.581898 5.1:+26675049 MS.gene45142:CDS
AACTTGGTAGCAATCAGTAA+TGG 0.589681 5.1:+26674636 MS.gene45142:CDS
AGTTCTTATCTTGTTTACAG+TGG 0.604622 5.1:+26675593 MS.gene45142:CDS
TCATCAAGATCACAAAATCC+GGG 0.616047 5.1:+26674713 MS.gene45142:CDS
GGTGGGGTTGATGATGATGA+TGG 0.618938 5.1:+26675569 MS.gene45142:CDS
TTCTGAATGAATGTGATCAT+GGG 0.626189 5.1:-26674379 None:intergenic
GATTGTTGTGAAGTGAGAGA+AGG 0.627747 5.1:-26674450 None:intergenic
GGAGACATAAGAAGTATACA+TGG 0.631337 5.1:+26674871 MS.gene45142:CDS
ATAGTACTAATCAAGATCAG+AGG 0.646621 5.1:+26674766 MS.gene45142:CDS
GAGACATAAGAAGTATACAT+GGG 0.687899 5.1:+26674872 MS.gene45142:CDS
ATGTGATCATGGGAAGAATG+AGG 0.690153 5.1:-26674369 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAGAAAAAGCATTTTT+TGG + chr5.1:26675163-26675182 MS.gene45142:CDS 15.0%
!!! AAATCTTCTTATTTCACTAT+TGG - chr5.1:26674574-26674593 None:intergenic 20.0%
!!! TGATAGATCAATTTTAGTTT+GGG + chr5.1:26675268-26675287 MS.gene45142:CDS 20.0%
! AAACTTCTAACAACAATCAA+TGG + chr5.1:26674489-26674508 MS.gene45142:CDS 25.0%
! CTGATAGAAAGATCAAAATT+TGG + chr5.1:26675090-26675109 MS.gene45142:CDS 25.0%
! CTGATTATAGTGTTAGATTA+TGG + chr5.1:26675438-26675457 MS.gene45142:CDS 25.0%
! TAAAAAAGCTGTTAAGTGTT+TGG + chr5.1:26675514-26675533 MS.gene45142:CDS 25.0%
! TGAAAAAAGTTATGCATGTT+TGG + chr5.1:26675475-26675494 MS.gene45142:CDS 25.0%
! TTCAAACAATATCAACAACT+TGG + chr5.1:26674620-26674639 MS.gene45142:CDS 25.0%
! TTGATTGTGATATCAAAGTT+TGG + chr5.1:26675621-26675640 MS.gene45142:CDS 25.0%
!! AAATTTAGAGTGTAAAGCAA+GGG - chr5.1:26674535-26674554 None:intergenic 25.0%
!! TTTTTCATCGAAAAATTACG+TGG + chr5.1:26674842-26674861 MS.gene45142:CDS 25.0%
!!! AAGAAAAAGCATTTTTTGGT+TGG + chr5.1:26675167-26675186 MS.gene45142:CDS 25.0%
!!! CTGTTTTTTGTTTTTGTTGT+TGG - chr5.1:26674347-26674366 None:intergenic 25.0%
!!! GTGATAGATCAATTTTAGTT+TGG + chr5.1:26675267-26675286 MS.gene45142:CDS 25.0%
AGCATTATTCGAAATTGTTC+CGG + chr5.1:26675302-26675321 MS.gene45142:CDS 30.0%
AGTGTTAGATTATGGAAAAG+AGG + chr5.1:26675446-26675465 MS.gene45142:CDS 30.0%
ATAGTACTAATCAAGATCAG+AGG + chr5.1:26674766-26674785 MS.gene45142:CDS 30.0%
ATTCTGAATGAATGTGATCA+TGG - chr5.1:26674383-26674402 None:intergenic 30.0%
CACTCTAAATTTCTCTTTAG+TGG + chr5.1:26674543-26674562 MS.gene45142:CDS 30.0%
GAAAAACGATCATTGAATGT+TGG - chr5.1:26674816-26674835 None:intergenic 30.0%
GATTCCAAAAGTGGTAAAAA+TGG + chr5.1:26675548-26675567 MS.gene45142:CDS 30.0%
GGGATAGAACATTCAAAATT+TGG + chr5.1:26674964-26674983 MS.gene45142:CDS 30.0%
TACTAAGGCTATATTGTGTT+TGG + chr5.1:26675382-26675401 MS.gene45142:CDS 30.0%
TATATTGTGTTTGGTTGTGA+TGG + chr5.1:26675391-26675410 MS.gene45142:CDS 30.0%
TTCTGAATGAATGTGATCAT+GGG - chr5.1:26674382-26674401 None:intergenic 30.0%
! AGTTCTTATCTTGTTTACAG+TGG + chr5.1:26675593-26675612 MS.gene45142:CDS 30.0%
! GAAATTTAGAGTGTAAAGCA+AGG - chr5.1:26674536-26674555 None:intergenic 30.0%
! GAGACATAAGAAGTATACAT+GGG + chr5.1:26674872-26674891 MS.gene45142:CDS 30.0%
! TTTTGTGTGTAGCAACATAA+AGG - chr5.1:26674324-26674343 None:intergenic 30.0%
!! AACAACAATCATTGACTTGA+TGG - chr5.1:26674673-26674692 None:intergenic 30.0%
!! AATTGTTGTTTCATCTTCTG+GGG + chr5.1:26675049-26675068 MS.gene45142:CDS 30.0%
!! AGATGGATTGTTTCTTTACT+CGG + chr5.1:26674935-26674954 MS.gene45142:CDS 30.0%
!! CAATTGTTGTTTCATCTTCT+GGG + chr5.1:26675048-26675067 MS.gene45142:CDS 30.0%
!! GATGGATCAGTTTTATATTC+AGG + chr5.1:26675239-26675258 MS.gene45142:CDS 30.0%
ATAGCAGATGATTCCAAAAG+TGG + chr5.1:26675539-26675558 MS.gene45142:CDS 35.0%
CAAAAGTGGTAAAAATGGTG+GGG + chr5.1:26675553-26675572 MS.gene45142:CDS 35.0%
CCAAAAGTGGTAAAAATGGT+GGG + chr5.1:26675552-26675571 MS.gene45142:CDS 35.0%
CTCATCAAGATCACAAAATC+CGG + chr5.1:26674712-26674731 MS.gene45142:CDS 35.0%
GCTTTAGCACTAAACAAAGA+TGG + chr5.1:26675221-26675240 MS.gene45142:CDS 35.0%
TCATCAAGATCACAAAATCC+GGG + chr5.1:26674713-26674732 MS.gene45142:CDS 35.0%
TCCAAAAGTGGTAAAAATGG+TGG + chr5.1:26675551-26675570 MS.gene45142:CDS 35.0%
TTGAAGCTTGAAGAGCAAAA+GGG - chr5.1:26674605-26674624 None:intergenic 35.0%
! AACTTGGTAGCAATCAGTAA+TGG + chr5.1:26674636-26674655 MS.gene45142:CDS 35.0%
! GGAGACATAAGAAGTATACA+TGG + chr5.1:26674871-26674890 MS.gene45142:CDS 35.0%
! TTGTTTCGATTTTCCAAACC+CGG - chr5.1:26674734-26674753 None:intergenic 35.0%
! TTTGAAGCTTGAAGAGCAAA+AGG - chr5.1:26674606-26674625 None:intergenic 35.0%
!! GCAATTGTTGTTTCATCTTC+TGG + chr5.1:26675047-26675066 MS.gene45142:CDS 35.0%
!! TTTGGTTGTGATGGATGATT+TGG + chr5.1:26675400-26675419 MS.gene45142:CDS 35.0%
AAGATCACAAAATCCGGGTT+TGG + chr5.1:26674718-26674737 MS.gene45142:CDS 40.0%
ATGTGATCATGGGAAGAATG+AGG - chr5.1:26674372-26674391 None:intergenic 40.0%
CGAAAAATTACGTGGAAGTC+AGG + chr5.1:26674850-26674869 MS.gene45142:CDS 40.0%
GACGAAAATATGGTCCTAGT+TGG + chr5.1:26675344-26675363 MS.gene45142:CDS 40.0%
GATTGTTGTGAAGTGAGAGA+AGG - chr5.1:26674453-26674472 None:intergenic 40.0%
GTAAAGCAAGGGATGAGATA+GGG - chr5.1:26674524-26674543 None:intergenic 40.0%
TAAAGCAAGGGATGAGATAG+GGG - chr5.1:26674523-26674542 None:intergenic 40.0%
TGTAAAGCAAGGGATGAGAT+AGG - chr5.1:26674525-26674544 None:intergenic 40.0%
!! ATGGATGATTTGGTGTGTAG+TGG + chr5.1:26675410-26675429 MS.gene45142:CDS 40.0%
!! CCCACCATTTTTACCACTTT+TGG - chr5.1:26675555-26675574 None:intergenic 40.0%
!! TCTCTTGCTGTTTCGAAAGA+TGG + chr5.1:26674918-26674937 MS.gene45142:CDS 40.0%
!! TCTTCTGGGGTTGTTTATAC+AGG + chr5.1:26675062-26675081 MS.gene45142:CDS 40.0%
AGACCCAGACGACGAAAATA+TGG + chr5.1:26675334-26675353 MS.gene45142:CDS 45.0%
AGCCTTAGTATGCCCTCTTA+GGG - chr5.1:26675372-26675391 None:intergenic 45.0%
GAAGGAACAGAAGGTAGACT+TGG - chr5.1:26674435-26674454 None:intergenic 45.0%
GAAGTGAGAGAAGGAACAGA+AGG - chr5.1:26674444-26674463 None:intergenic 45.0%
GTTTCTTTACTCGGCTTCGT+GGG + chr5.1:26674944-26674963 MS.gene45142:CDS 45.0%
TGTTTCTTTACTCGGCTTCG+TGG + chr5.1:26674943-26674962 MS.gene45142:CDS 45.0%
! AGGACCATATTTTCGTCGTC+TGG - chr5.1:26675341-26675360 None:intergenic 45.0%
! GGACCATATTTTCGTCGTCT+GGG - chr5.1:26675340-26675359 None:intergenic 45.0%
! TAGCCTTAGTATGCCCTCTT+AGG - chr5.1:26675373-26675392 None:intergenic 45.0%
! TATTTTCGTCGTCTGGGTCT+TGG - chr5.1:26675334-26675353 None:intergenic 45.0%
!! GCATGTTTGGCTGTTTTGCA+AGG + chr5.1:26675488-26675507 MS.gene45142:CDS 45.0%
! TCCTAGTTGGTGCCCTAAGA+GGG + chr5.1:26675357-26675376 MS.gene45142:CDS 50.0%
! TGCCCTAAGAGGGCATACTA+AGG + chr5.1:26675367-26675386 MS.gene45142:CDS 50.0%
!! GGTGGGGTTGATGATGATGA+TGG + chr5.1:26675569-26675588 MS.gene45142:CDS 50.0%
GTCTGGGTCTTGGTCTAGTC+CGG - chr5.1:26675324-26675343 None:intergenic 55.0%
! GTCCTAGTTGGTGCCCTAAG+AGG + chr5.1:26675356-26675375 MS.gene45142:CDS 55.0%
! GCCCTCTTAGGGCACCAACT+AGG - chr5.1:26675361-26675380 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 26674303 26675667 26674303 ID=MS.gene45142
chr5.1 mRNA 26674303 26675667 26674303 ID=MS.gene45142.t1;Parent=MS.gene45142
chr5.1 exon 26674303 26675667 26674303 ID=MS.gene45142.t1.exon1;Parent=MS.gene45142.t1
chr5.1 CDS 26674303 26675667 26674303 ID=cds.MS.gene45142.t1;Parent=MS.gene45142.t1
Gene Sequence

>MS.gene45142

ATGGTACTTCTTCAATGTCCTTTATGTTGCTACACACAAAACCAACAACAAAAACAAAAAACAGAACCTCATTCTTCCCATGATCACATTCATTCAGAATCATCAACTTCATCATCTCTTTCTTCACAACCAAGTCTACCTTCTGTTCCTTCTCTCACTTCACAACAATCTCAAAGCACACATCACAAACTTCTAACAACAATCAATGGTCACTCTTCCCCTATCTCATCCCTTGCTTTACACTCTAAATTTCTCTTTAGTGGTTCTTCCAATAGTGAAATAAGAAGATTTGACAAAGACCCTTTTGCTCTTCAAGCTTCAAACAATATCAACAACTTGGTAGCAATCAGTAATGGATCAAAATCAACCATCAAGTCAATGATTGTTGTTAATGATATGTTATTTAGTGCTCATCAAGATCACAAAATCCGGGTTTGGAAAATCGAAACAACGACAGCTAGTAATAGTACTAATCAAGATCAGAGGCTATTTAAATGCATAGCAACACTTCCAACATTCAATGATCGTTTTTCGAAGCTTTTTTCATCGAAAAATTACGTGGAAGTCAGGAGACATAAGAAGTATACATGGGTGAATCATATTGATGCTGTTTCTTCTCTTGCTGTTTCGAAAGATGGATTGTTTCTTTACTCGGCTTCGTGGGATAGAACATTCAAAATTTGGAGAGTTTCAGATTTCAAGTGTTTAGAGTCAGTGAAAAATGCACATGAAGATGCAATCAATGCAATTGTTGTTTCATCTTCTGGGGTTGTTTATACAGGTTCAGCTGATAGAAAGATCAAAATTTGGAAGAAAAAAAATCTTGAAGAAGATGAGAAAATGAAAAGTAAAAGTAAAAGTAAAAAGAAAAAGCATTTTTTGGTTGGAACATTAGAGAAGCATAAATCAGCTGTGAATGCTTTAGCACTAAACAAAGATGGATCAGTTTTATATTCAGGTGCATGTGATAGATCAATTTTAGTTTGGGAAAAAAGTAATAGCATTATTCGAAATTGTTCCGGACTAGACCAAGACCCAGACGACGAAAATATGGTCCTAGTTGGTGCCCTAAGAGGGCATACTAAGGCTATATTGTGTTTGGTTGTGATGGATGATTTGGTGTGTAGTGGATCAGCTGATTATAGTGTTAGATTATGGAAAAGAGGAATTGATGAAAAAAGTTATGCATGTTTGGCTGTTTTGCAAGGTCATAAAAAAGCTGTTAAGTGTTTGGCTATAGCAGATGATTCCAAAAGTGGTAAAAATGGTGGGGTTGATGATGATGATGGTAGTTCTTATCTTGTTTACAGTGGTAGTCTTGATTGTGATATCAAAGTTTGGCAAATACGCGTTCCCTTAATTTGA

Protein sequence

>MS.gene45142.t1

MVLLQCPLCCYTQNQQQKQKTEPHSSHDHIHSESSTSSSLSSQPSLPSVPSLTSQQSQSTHHKLLTTINGHSSPISSLALHSKFLFSGSSNSEIRRFDKDPFALQASNNINNLVAISNGSKSTIKSMIVVNDMLFSAHQDHKIRVWKIETTTASNSTNQDQRLFKCIATLPTFNDRFSKLFSSKNYVEVRRHKKYTWVNHIDAVSSLAVSKDGLFLYSASWDRTFKIWRVSDFKCLESVKNAHEDAINAIVVSSSGVVYTGSADRKIKIWKKKNLEEDEKMKSKSKSKKKKHFLVGTLEKHKSAVNALALNKDGSVLYSGACDRSILVWEKSNSIIRNCSGLDQDPDDENMVLVGALRGHTKAILCLVVMDDLVCSGSADYSVRLWKRGIDEKSYACLAVLQGHKKAVKCLAIADDSKSGKNGGVDDDDGSSYLVYSGSLDCDIKVWQIRVPLI