AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45463

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45463.t1 MTR_4g069570 90.801 337 25 2 111 447 6 336 0.0 625
MS.gene45463.t1 MTR_4g069550 62.448 482 127 11 1 447 1 463 0.0 572
MS.gene45463.t1 MTR_4g069520 61.957 460 113 6 41 447 273 723 0.0 561
MS.gene45463.t1 MTR_4g069520 42.345 307 58 8 1 197 1 298 9.37e-53 189
MS.gene45463.t1 MTR_4g069520 45.415 229 54 5 41 198 179 407 3.09e-44 165
MS.gene45463.t1 MTR_4g069520 61.957 460 113 6 41 447 259 709 0.0 560
MS.gene45463.t1 MTR_4g069520 52.439 246 59 6 1 197 1 237 8.69e-63 216
MS.gene45463.t1 MTR_4g069520 45.415 229 54 5 41 198 165 393 3.76e-44 164
MS.gene45463.t1 MTR_4g069040 57.270 337 121 5 118 446 188 509 1.48e-131 389
MS.gene45463.t1 MTR_3g116320 42.069 435 215 11 36 447 268 688 8.57e-102 318
MS.gene45463.t1 MTR_3g116270 36.731 520 242 16 1 447 1 506 9.45e-101 310
MS.gene45463.t1 MTR_6g086530 71.220 205 58 1 243 446 1 205 4.02e-97 290
MS.gene45463.t1 MTR_3g107830 46.575 219 112 4 231 446 39 255 1.02e-67 217
MS.gene45463.t1 MTR_3g116410 46.575 219 112 4 231 446 59 275 1.91e-67 217
MS.gene45463.t1 MTR_4g069680 45.662 219 114 4 231 446 70 286 1.31e-65 213
MS.gene45463.t1 MTR_3g116380 45.000 220 115 4 231 447 87 303 2.56e-64 209
MS.gene45463.t1 MTR_4g068420 62.590 139 33 1 309 447 2 121 5.09e-60 192
MS.gene45463.t1 MTR_4g069540 89.412 85 9 0 210 294 13 97 7.76e-46 154
MS.gene45463.t1 MTR_1g008500 38.813 219 124 6 231 445 171 383 3.56e-45 161
MS.gene45463.t1 MTR_1g008510 37.615 218 129 5 229 445 113 324 1.36e-42 153
MS.gene45463.t1 MTR_3g109490 40.455 220 122 7 231 446 107 321 2.54e-42 152
MS.gene45463.t1 MTR_0312s0030 38.073 218 125 7 231 445 113 323 2.93e-42 152
MS.gene45463.t1 MTR_1g007520 38.073 218 125 7 231 445 113 323 2.93e-42 152
MS.gene45463.t1 MTR_1g008590 38.073 218 125 7 231 445 113 323 1.05e-41 150
MS.gene45463.t1 MTR_1g008530 36.818 220 124 7 233 445 11 222 2.05e-41 147
MS.gene45463.t1 MTR_1g008470 35.484 217 131 5 231 445 89 298 4.28e-41 148
MS.gene45463.t1 MTR_1g008460 36.279 215 129 6 231 445 89 295 1.19e-40 147
MS.gene45463.t1 MTR_1g008560 36.486 222 130 6 229 445 113 328 3.65e-40 147
MS.gene45463.t1 MTR_1g008450 36.019 211 126 6 236 444 107 310 1.43e-35 134
MS.gene45463.t1 MTR_1g008580 35.795 176 106 5 271 445 1 170 5.25e-33 123
MS.gene45463.t1 MTR_3g078090 33.182 220 133 6 231 444 413 624 3.81e-31 126
MS.gene45463.t1 MTR_5g034320 30.734 218 141 6 231 444 415 626 4.62e-29 120
MS.gene45463.t1 MTR_8g064560 30.415 217 143 5 231 444 412 623 3.03e-27 115
MS.gene45463.t1 MTR_8g064500 30.126 239 151 7 209 444 145 370 2.15e-26 110
MS.gene45463.t1 MTR_8g064530 29.032 217 146 5 231 444 429 640 1.23e-24 107
MS.gene45463.t1 MTR_4g133840 32.620 187 118 7 260 444 6 186 5.89e-23 96.3
MS.gene45463.t1 MTR_5g033350 28.191 188 122 4 265 444 16 198 1.18e-19 87.4
MS.gene45463.t1 MTR_5g033340 26.364 220 154 5 228 444 163 377 1.12e-18 87.8
MS.gene45463.t1 MTR_5g033340 28.859 149 102 2 276 424 6 150 4.92e-16 79.7
MS.gene45463.t1 MTR_2g081770 30.962 239 133 12 211 446 61 270 3.48e-17 82.0
MS.gene45463.t1 MTR_8g046000 36.364 154 84 6 295 447 83 223 3.97e-17 80.9
MS.gene45463.t1 MTR_5g033330 26.500 200 140 4 228 424 194 389 6.64e-17 83.6
MS.gene45463.t1 MTR_5g033330 27.976 168 109 3 265 424 18 181 4.03e-16 81.3
MS.gene45463.t1 MTR_5g033330 25.455 220 156 5 228 444 402 616 9.57e-16 80.1
MS.gene45463.t1 MTR_8g445140 34.667 150 86 7 297 446 165 302 2.69e-16 79.7
MS.gene45463.t1 MTR_8g045890 34.667 150 86 7 297 446 294 431 5.09e-16 80.1
MS.gene45463.t1 MTR_8g045890 34.667 150 86 7 297 446 335 472 5.89e-16 80.1
MS.gene45463.t1 MTR_8g045890 34.667 150 86 7 297 446 372 509 6.05e-16 80.5
MS.gene45463.t1 MTR_8g445170 32.500 160 96 7 287 446 236 383 1.09e-15 79.0
MS.gene45463.t1 MTR_8g045880 34.667 150 86 6 297 446 177 314 1.86e-14 74.7
MS.gene45463.t1 MTR_8g045880 34.667 150 86 6 297 446 212 349 2.52e-14 74.7
MS.gene45463.t1 MTR_8g045880 34.667 150 86 6 297 446 214 351 2.91e-14 74.3
MS.gene45463.t1 MTR_2g081610 33.566 143 83 6 304 446 129 259 1.44e-13 71.2
MS.gene45463.t1 MTR_2g081590 27.386 241 140 12 208 446 52 259 1.10e-12 68.6
MS.gene45463.t1 MTR_8g075930 40.278 72 41 2 358 428 11 81 8.66e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45463.t1 AT1G49320 37.387 222 131 3 231 445 58 278 4.77e-52 177
MS.gene45463.t1 AT5G25610 36.652 221 128 7 231 445 176 390 1.71e-41 152
MS.gene45463.t1 AT1G60390 27.027 407 246 18 62 444 241 620 2.41e-26 112
MS.gene45463.t1 AT1G70370 26.905 420 261 18 48 444 226 622 5.42e-25 108
MS.gene45463.t1 AT1G70370 26.905 420 261 18 48 444 226 622 5.42e-25 108
MS.gene45463.t1 AT1G23760 29.319 191 129 4 255 444 433 618 1.97e-23 103

Find 132 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAGCTCCGTTGGATTTA+TGG 0.143157 4.1:-31938382 None:intergenic
TTTGATCTCCGTTGGATTTA+TGG 0.163493 4.1:-31938115 None:intergenic
GAATTTGTTGACACAATTAT+TGG 0.204523 4.1:+31938818 MS.gene45463:CDS
ATTCAGACAACTTGGGATTA+AGG 0.231832 4.1:+31939123 MS.gene45463:CDS
GTATGATCCAAGTTAGAAAT+TGG 0.234762 4.1:-31938515 None:intergenic
CTCCGTTGGATTTATGGTTA+TGG 0.234942 4.1:-31938109 None:intergenic
TTGTACCCAGTAATGTAATT+TGG 0.253989 4.1:-31938437 None:intergenic
CTATACCCGGTAATGTAATT+TGG 0.262932 4.1:-31938083 None:intergenic
TCCGTTGGATTTATGGTTAT+GGG 0.267348 4.1:-31938108 None:intergenic
TTCATTTGATTCTGGTTGTT+TGG 0.269582 4.1:-31938021 None:intergenic
TTCATTTGATTCTGGTTGTT+TGG 0.269582 4.1:-31938186 None:intergenic
AAACTTCTTGGACCGGAAAT+TGG 0.272020 4.1:-31938601 None:intergenic
TGTAATGACCCTCCAATTTC+CGG 0.278512 4.1:+31938589 MS.gene45463:CDS
TGATGAGAATGGAGATAAAA+TGG 0.280906 4.1:+31939045 MS.gene45463:CDS
CTCCGTTGGATTTATGGTTA+TGG 0.282351 4.1:-31938376 None:intergenic
ATGTAAGGTTCATTTGATTC+TGG 0.296295 4.1:-31938029 None:intergenic
ATGTAAGGTTCATTTGATTC+TGG 0.296295 4.1:-31938194 None:intergenic
TAGTAAATGGCATATGGATA+TGG 0.303048 4.1:-31938971 None:intergenic
TGTCTGAATATAATATGATT+AGG 0.304357 4.1:-31939109 None:intergenic
AGTTCTAGGTGAGGGGTTGC+TGG 0.311022 4.1:-31938870 None:intergenic
AAGTGCTCCTCTCATGGATT+TGG 0.314509 4.1:-31938726 None:intergenic
CAAAATCAAGTGGATTTAAA+TGG 0.315181 4.1:+31937969 MS.gene45463:CDS
TCCGTTGGATTTATGGTTAT+GGG 0.315611 4.1:-31938375 None:intergenic
GAACCTTACATAACTGGATA+TGG 0.323449 4.1:+31938041 MS.gene45463:CDS
GAACCTTACATAACTGGATA+TGG 0.323449 4.1:+31938206 MS.gene45463:CDS
GGTAATGTAATTTGATTGTT+TGG 0.326516 4.1:-31938240 None:intergenic
CAATATCGTATAACTCAATA+TGG 0.334238 4.1:+31938308 MS.gene45463:CDS
CTTGAACACTTTAGTCTTTG+TGG 0.337552 4.1:-31939008 None:intergenic
TCCATTCAGATGTATCAAAA+TGG 0.341451 4.1:-31939084 None:intergenic
GACCATTGGATTCAAGGTTA+TGG 0.344436 4.1:-31938274 None:intergenic
TCAAATGAACCTTACATAAC+TGG 0.344743 4.1:+31938035 MS.gene45463:CDS
TCAAATGAACCTTACATAAC+TGG 0.344743 4.1:+31938200 MS.gene45463:CDS
TGCGAAGGGGAAACCGTTAC+AGG 0.345500 4.1:+31938755 MS.gene45463:CDS
AGAGGTAAGGAGAAACTTCT+TGG 0.347982 4.1:-31938613 None:intergenic
AAGCAACCAAATTACATTAC+TGG 0.350332 4.1:+31938431 MS.gene45463:CDS
GGTAATGTAATTTGGTTGTT+TGG 0.352526 4.1:-31938075 None:intergenic
TTGGCTTGAGGAGATCCTTC+AGG 0.355790 4.1:-31938707 None:intergenic
ACCATTGGATTCAAGGTTAT+GGG 0.355863 4.1:-31938273 None:intergenic
GTCTGAATATAATATGATTA+GGG 0.381294 4.1:-31939108 None:intergenic
AAACAATCAAATTACATTAC+CGG 0.386224 4.1:+31938242 MS.gene45463:CDS
AAGCAATCAAATTACATTAC+CGG 0.386224 4.1:+31938344 MS.gene45463:CDS
AATGTAATGGCAATAGTAAA+TGG 0.389637 4.1:-31938984 None:intergenic
AAACAACCAAATTACATTAC+CGG 0.395982 4.1:+31938077 MS.gene45463:CDS
TGTAAGGACCATTGGATTCA+AGG 0.402435 4.1:-31938280 None:intergenic
CACAAAGACTAAAGTGTTCA+AGG 0.405308 4.1:+31939009 MS.gene45463:CDS
GAAAAGAGTTGAAGAATACT+TGG 0.405738 4.1:-31938680 None:intergenic
GGATGACAAGCTACCCATTT+GGG 0.407784 4.1:-31938944 None:intergenic
CATATTATATTCAGACAACT+TGG 0.410545 4.1:+31939115 MS.gene45463:CDS
AGATCTCCCAATTTCTAACT+TGG 0.414347 4.1:+31938508 MS.gene45463:CDS
GGTAATGTAATTTGATTGCT+TGG 0.415162 4.1:-31938342 None:intergenic
CAAGACAGGATATTTCAGTT+TGG 0.426765 4.1:+31938547 MS.gene45463:CDS
CTCTTCAAGTGCTCCTCTCA+TGG 0.434563 4.1:-31938732 None:intergenic
CAAATTACATTACCGGGTAT+AGG 0.434802 4.1:+31938084 MS.gene45463:CDS
CAAATTACATTACCGGGTAT+AGG 0.434802 4.1:+31938351 MS.gene45463:CDS
TTGAGTTATGTAAGGACCAT+TGG 0.436047 4.1:-31938288 None:intergenic
ATGATATTGATGTTCCCAAA+TGG 0.438419 4.1:+31938930 MS.gene45463:CDS
TTACCATATCCAGTTATGTA+AGG 0.446290 4.1:-31938044 None:intergenic
TTACCATATCCAGTTATGTA+AGG 0.446290 4.1:-31938209 None:intergenic
CCTCTCATGGATTTGGCTTG+AGG 0.446591 4.1:-31938719 None:intergenic
GGGATGACAAGCTACCCATT+TGG 0.447559 4.1:-31938945 None:intergenic
TCCCTGTGAACCATCTTATG+TGG 0.449193 4.1:+31939176 MS.gene45463:CDS
GAGCATGGACTCTAGAGAAT+TGG 0.449401 4.1:-31938795 None:intergenic
TAGTCTTTGTGGCAATGTAA+TGG 0.454439 4.1:-31938997 None:intergenic
TGGCAATAGTAAATGGCATA+TGG 0.457847 4.1:-31938977 None:intergenic
GGAGATAAAATGGAAGCTCT+TGG 0.462321 4.1:+31939055 MS.gene45463:CDS
AGACAACTTGGGATTAAGGC+TGG 0.463334 4.1:+31939127 MS.gene45463:CDS
ATACGATATTGAGTTATGTA+AGG 0.463612 4.1:-31938296 None:intergenic
TATGATCCAAGTTAGAAATT+GGG 0.467513 4.1:-31938514 None:intergenic
TGGCTTGAGGAGATCCTTCA+GGG 0.473228 4.1:-31938706 None:intergenic
CTTCTACGGAGTTGGTCGAT+GGG 0.477729 4.1:-31938487 None:intergenic
TGAAGTTATGTAAGCTCCGT+TGG 0.478150 4.1:-31938390 None:intergenic
ATGATTATAAGCAGTTATGT+AGG 0.479774 4.1:-31937994 None:intergenic
ATGATTATAAGCAGTTATGT+AGG 0.479774 4.1:-31938159 None:intergenic
CAAAATCAAGTGGATTCAAA+TGG 0.483359 4.1:+31938134 MS.gene45463:CDS
TGATATTGATGTTCCCAAAT+GGG 0.483777 4.1:+31938931 MS.gene45463:CDS
TGGGAGATCTTCTACGGAGT+TGG 0.494365 4.1:-31938495 None:intergenic
TATGATGTCATTGATCCAAA+AGG 0.494967 4.1:+31938461 MS.gene45463:CDS
GAGTTACCATAAAGAAGAAA+AGG 0.502080 4.1:-31937146 None:intergenic
AGTAAATGGCATATGGATAT+GGG 0.502163 4.1:-31938970 None:intergenic
TGGCTTTGGGATGGCTACAT+TGG 0.503222 4.1:-31939202 None:intergenic
ATCATGTGATACTTCTAAAA+CGG 0.508731 4.1:-31938912 None:intergenic
AGAGGGATAGTTCTAGGTGA+GGG 0.508920 4.1:-31938878 None:intergenic
TCTTCTACGGAGTTGGTCGA+TGG 0.511805 4.1:-31938488 None:intergenic
GAGCACTTGAAGAGTGCGAA+GGG 0.516914 4.1:+31938741 MS.gene45463:CDS
CTTCTTGGACCGGAAATTGG+AGG 0.517939 4.1:-31938598 None:intergenic
TTCTACGGAGTTGGTCGATG+GGG 0.525922 4.1:-31938486 None:intergenic
AGAAATTGGGAGATCTTCTA+CGG 0.530034 4.1:-31938501 None:intergenic
GGTCTTTGTCTCCCCTGTAA+CGG 0.530396 4.1:-31938768 None:intergenic
GGGAGTTGTGACATCGAGAA+AGG 0.530459 4.1:-31938659 None:intergenic
CATACAGAAGCTTTCAAGAC+AGG 0.532423 4.1:+31938533 MS.gene45463:CDS
AACAACCAAATTACATTACC+GGG 0.532937 4.1:+31938078 MS.gene45463:CDS
AGCAACCAAATTACATTACT+GGG 0.535052 4.1:+31938432 MS.gene45463:CDS
TCTACGGAGTTGGTCGATGG+GGG 0.538983 4.1:-31938485 None:intergenic
AAAAGAGTTGAAGAATACTT+GGG 0.539336 4.1:-31938679 None:intergenic
GACAACTTGGGATTAAGGCT+GGG 0.540548 4.1:+31939128 MS.gene45463:CDS
ACATTGGAATCCACATAAGA+TGG 0.540746 4.1:-31939186 None:intergenic
AACAATCAAATTACATTACC+GGG 0.544310 4.1:+31938243 MS.gene45463:CDS
ACATCATTGTAGATTGGCAG+AGG 0.551845 4.1:+31937909 MS.gene45463:intron
ATGGTTATGGGTCCTATACC+CGG 0.552015 4.1:-31938096 None:intergenic
ATGGTTATGGGTCCTATACC+CGG 0.552015 4.1:-31938363 None:intergenic
TCACTTGTTAGTGATGAGAA+TGG 0.555520 4.1:+31939034 MS.gene45463:CDS
TAAGGAGAAACTTCTTGGAC+CGG 0.557579 4.1:-31938608 None:intergenic
ATCCACATAAGATGGTTCAC+AGG 0.559204 4.1:-31939178 None:intergenic
ATATTATATTCAGACAACTT+GGG 0.559590 4.1:+31939116 MS.gene45463:CDS
TCTAGGTGAGGGGTTGCTGG+TGG 0.565202 4.1:-31938867 None:intergenic
ACTTCATATGATAAACATGA+AGG 0.566668 4.1:+31938407 MS.gene45463:CDS
TTCTTGGACCGGAAATTGGA+GGG 0.569528 4.1:-31938597 None:intergenic
AGTTTGGATGATCTTCACGT+TGG 0.576107 4.1:+31938563 MS.gene45463:CDS
TAGAGGGATAGTTCTAGGTG+AGG 0.581869 4.1:-31938879 None:intergenic
TTGTGACATCGAGAAAGGAA+TGG 0.584970 4.1:-31938654 None:intergenic
GCTGCGAGATCAAAATCAAG+TGG 0.587722 4.1:+31937959 MS.gene45463:CDS
GCGAAGGGGAAACCGTTACA+GGG 0.589433 4.1:+31938756 MS.gene45463:CDS
GGAGCACTTGAAGAGTGCGA+AGG 0.589477 4.1:+31938740 MS.gene45463:CDS
ACCCATAACCTTGAATCCAA+TGG 0.589959 4.1:+31938272 MS.gene45463:CDS
CAACGGAGATCAAAATCAAG+TGG 0.591165 4.1:+31938124 MS.gene45463:CDS
AGCAATCAAATTACATTACC+GGG 0.594581 4.1:+31938345 MS.gene45463:CDS
AAATGGCATATGGATATGGG+AGG 0.595703 4.1:-31938967 None:intergenic
CTACGGAGTTGGTCGATGGG+GGG 0.604712 4.1:-31938484 None:intergenic
AAGGTTATGGGTCTTATACC+CGG 0.607957 4.1:-31938261 None:intergenic
ATGGCATATGGATATGGGAG+GGG 0.608580 4.1:-31938965 None:intergenic
TGTGTCAACAAATTCGAGCA+TGG 0.613242 4.1:-31938810 None:intergenic
GAGGGATAGTTCTAGGTGAG+GGG 0.618840 4.1:-31938877 None:intergenic
ACCCATAACCATAAATCCAA+CGG 0.633019 4.1:+31938107 MS.gene45463:CDS
ACCCATAACCATAAATCCAA+CGG 0.633019 4.1:+31938374 MS.gene45463:CDS
GGACTCTAGAGAATTGGCAC+AGG 0.635769 4.1:-31938789 None:intergenic
CATCGAGAAAGGAATGGAGT+CGG 0.647174 4.1:-31938648 None:intergenic
CCTCAAGCCAAATCCATGAG+AGG 0.648458 4.1:+31938719 MS.gene45463:CDS
TCCACATAAGATGGTTCACA+GGG 0.658935 4.1:-31939177 None:intergenic
CGAAGGGGAAACCGTTACAG+GGG 0.669441 4.1:+31938757 MS.gene45463:CDS
AGCACTTGAAGAGTGCGAAG+GGG 0.678572 4.1:+31938742 MS.gene45463:CDS
ACAGGGAAGAAATGACACAC+TGG 0.683295 4.1:-31939160 None:intergenic
AATGGCATATGGATATGGGA+GGG 0.696886 4.1:-31938966 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG - chr4.1:31937583-31937564 None:intergenic 0.0%
!! TATATATATATATATATATA+GGG - chr4.1:31937582-31937563 None:intergenic 0.0%
!! ACCTTAAAAAAAATTAAAAG+TGG - chr4.1:31937507-31937526 None:intergenic 15.0%
!!! TCCACTTTTAATTTTTTTTA+AGG + chr4.1:31937503-31937522 MS.gene45463:intron 15.0%
!!! TTTCAATTAATTTTTCTTTG+AGG + chr4.1:31937689-31937708 MS.gene45463:intron 15.0%
!! AAACAATCAAATTACATTAC+CGG + chr4.1:31938242-31938261 MS.gene45463:CDS 20.0%
!! ATATTATATTCAGACAACTT+GGG + chr4.1:31939116-31939135 MS.gene45463:CDS 20.0%
!! GTCTGAATATAATATGATTA+GGG - chr4.1:31939111-31939130 None:intergenic 20.0%
!! TAGTAATAGTGATATCAATT+TGG + chr4.1:31937789-31937808 MS.gene45463:intron 20.0%
!! TGTCTGAATATAATATGATT+AGG - chr4.1:31939112-31939131 None:intergenic 20.0%
!!! AGGACATGATATTTTTTTTT+TGG + chr4.1:31937457-31937476 MS.gene45463:intron 20.0%
! AAAAGAGTTGAAGAATACTT+GGG - chr4.1:31938682-31938701 None:intergenic 25.0%
! AAACAACCAAATTACATTAC+CGG + chr4.1:31938077-31938096 MS.gene45463:CDS 25.0%
! AACAATCAAATTACATTACC+GGG + chr4.1:31938243-31938262 MS.gene45463:CDS 25.0%
! AAGCAATCAAATTACATTAC+CGG + chr4.1:31938344-31938363 MS.gene45463:CDS 25.0%
! AATGTAATGGCAATAGTAAA+TGG - chr4.1:31938987-31939006 None:intergenic 25.0%
! ACTTCATATGATAAACATGA+AGG + chr4.1:31938407-31938426 MS.gene45463:CDS 25.0%
! ATACGATATTGAGTTATGTA+AGG - chr4.1:31938299-31938318 None:intergenic 25.0%
! ATCAATTTGGCTAATCAATT+GGG + chr4.1:31937802-31937821 MS.gene45463:intron 25.0%
! ATGATTATAAGCAGTTATGT+AGG - chr4.1:31937997-31938016 None:intergenic 25.0%
! CAATATCGTATAACTCAATA+TGG + chr4.1:31938308-31938327 MS.gene45463:CDS 25.0%
! CATATTATATTCAGACAACT+TGG + chr4.1:31939115-31939134 MS.gene45463:CDS 25.0%
! GGTAATGTAATTTGATTGTT+TGG - chr4.1:31938243-31938262 None:intergenic 25.0%
! TAGTTACTAACTCAGTTATA+GGG + chr4.1:31937189-31937208 MS.gene45463:intron 25.0%
! TATCAATTTGGCTAATCAAT+TGG + chr4.1:31937801-31937820 MS.gene45463:intron 25.0%
! TATGATCCAAGTTAGAAATT+GGG - chr4.1:31938517-31938536 None:intergenic 25.0%
! TTAGTTACTAACTCAGTTAT+AGG + chr4.1:31937188-31937207 MS.gene45463:intron 25.0%
!! ATCATGTGATACTTCTAAAA+CGG - chr4.1:31938915-31938934 None:intergenic 25.0%
!! ATTTGGTTCAAATATCACAA+AGG + chr4.1:31937237-31937256 MS.gene45463:intron 25.0%
!! CAAAATCAAGTGGATTTAAA+TGG + chr4.1:31937969-31937988 MS.gene45463:CDS 25.0%
!! TTTAGTCCTTTTCTTCTTTA+TGG + chr4.1:31937140-31937159 MS.gene45463:CDS 25.0%
!! TTTGGTTCAAATATCACAAA+GGG + chr4.1:31937238-31937257 MS.gene45463:intron 25.0%
!!! GAATTTGTTGACACAATTAT+TGG + chr4.1:31938818-31938837 MS.gene45463:CDS 25.0%
AACAACCAAATTACATTACC+GGG + chr4.1:31938078-31938097 MS.gene45463:CDS 30.0%
AAGCAACCAAATTACATTAC+TGG + chr4.1:31938431-31938450 MS.gene45463:CDS 30.0%
AGCAACCAAATTACATTACT+GGG + chr4.1:31938432-31938451 MS.gene45463:CDS 30.0%
AGCAATCAAATTACATTACC+GGG + chr4.1:31938345-31938364 MS.gene45463:CDS 30.0%
AGTAAATGGCATATGGATAT+GGG - chr4.1:31938973-31938992 None:intergenic 30.0%
ATGATATTGATGTTCCCAAA+TGG + chr4.1:31938930-31938949 MS.gene45463:CDS 30.0%
ATGTAAGGTTCATTTGATTC+TGG - chr4.1:31938032-31938051 None:intergenic 30.0%
GAAAAGAGTTGAAGAATACT+TGG - chr4.1:31938683-31938702 None:intergenic 30.0%
GAGTTACCATAAAGAAGAAA+AGG - chr4.1:31937149-31937168 None:intergenic 30.0%
GGTAATGTAATTTGATTGCT+TGG - chr4.1:31938345-31938364 None:intergenic 30.0%
GGTAATGTAATTTGGTTGTT+TGG - chr4.1:31938078-31938097 None:intergenic 30.0%
GTATGATCCAAGTTAGAAAT+TGG - chr4.1:31938518-31938537 None:intergenic 30.0%
TAGTAAATGGCATATGGATA+TGG - chr4.1:31938974-31938993 None:intergenic 30.0%
TATGAAATATTCATACCGAC+AGG + chr4.1:31937612-31937631 MS.gene45463:intron 30.0%
TATGATGTCATTGATCCAAA+AGG + chr4.1:31938461-31938480 MS.gene45463:CDS 30.0%
TCAAATGAACCTTACATAAC+TGG + chr4.1:31938035-31938054 MS.gene45463:CDS 30.0%
TCATTGACATCATTGTAGAT+TGG + chr4.1:31937903-31937922 MS.gene45463:intron 30.0%
TCCATTCAGATGTATCAAAA+TGG - chr4.1:31939087-31939106 None:intergenic 30.0%
TCTAAATTCACAAGGACATA+CGG + chr4.1:31937635-31937654 MS.gene45463:intron 30.0%
TGATATTGATGTTCCCAAAT+GGG + chr4.1:31938931-31938950 MS.gene45463:CDS 30.0%
TGATGAGAATGGAGATAAAA+TGG + chr4.1:31939045-31939064 MS.gene45463:CDS 30.0%
TTAATTGCAAGGACTTTCAA+GGG - chr4.1:31937756-31937775 None:intergenic 30.0%
TTACCATATCCAGTTATGTA+AGG - chr4.1:31938047-31938066 None:intergenic 30.0%
TTGTACCCAGTAATGTAATT+TGG - chr4.1:31938440-31938459 None:intergenic 30.0%
TTTAATTGCAAGGACTTTCA+AGG - chr4.1:31937757-31937776 None:intergenic 30.0%
! CAAAATCAAGTGGATTCAAA+TGG + chr4.1:31938134-31938153 MS.gene45463:CDS 30.0%
! TTCATTTGATTCTGGTTGTT+TGG - chr4.1:31938024-31938043 None:intergenic 30.0%
! TTTAGAACCTAGAGCTTTAT+GGG - chr4.1:31937724-31937743 None:intergenic 30.0%
!!! TACATCACGTTTTAATTGCA+AGG - chr4.1:31937767-31937786 None:intergenic 30.0%
ACATTGGAATCCACATAAGA+TGG - chr4.1:31939189-31939208 None:intergenic 35.0%
ACCATTGGATTCAAGGTTAT+GGG - chr4.1:31938276-31938295 None:intergenic 35.0%
ACCCATAACCATAAATCCAA+CGG + chr4.1:31938107-31938126 MS.gene45463:CDS 35.0%
AGAAATTGGGAGATCTTCTA+CGG - chr4.1:31938504-31938523 None:intergenic 35.0%
AGATCTCCCAATTTCTAACT+TGG + chr4.1:31938508-31938527 MS.gene45463:CDS 35.0%
ATTCAGACAACTTGGGATTA+AGG + chr4.1:31939123-31939142 MS.gene45463:CDS 35.0%
CAAATTACATTACCGGGTAT+AGG + chr4.1:31938084-31938103 MS.gene45463:CDS 35.0%
CAAGACAGGATATTTCAGTT+TGG + chr4.1:31938547-31938566 MS.gene45463:CDS 35.0%
CTATACCCGGTAATGTAATT+TGG - chr4.1:31938086-31938105 None:intergenic 35.0%
GAACCTTACATAACTGGATA+TGG + chr4.1:31938041-31938060 MS.gene45463:CDS 35.0%
GTTCAAATATCACAAAGGGA+GGG + chr4.1:31937242-31937261 MS.gene45463:intron 35.0%
TAATTGCAAGGACTTTCAAG+GGG - chr4.1:31937755-31937774 None:intergenic 35.0%
TAGTCTTTGTGGCAATGTAA+TGG - chr4.1:31939000-31939019 None:intergenic 35.0%
TCATGTCCTACAAAACTCTA+CGG - chr4.1:31937446-31937465 None:intergenic 35.0%
TCCGTTGGATTTATGGTTAT+GGG - chr4.1:31938111-31938130 None:intergenic 35.0%
TCCTTACCTCTCAAAAAAAG+TGG + chr4.1:31938625-31938644 MS.gene45463:CDS 35.0%
TGGCAATAGTAAATGGCATA+TGG - chr4.1:31938980-31938999 None:intergenic 35.0%
TTGAGTTATGTAAGGACCAT+TGG - chr4.1:31938291-31938310 None:intergenic 35.0%
TTTGATCTCCGTTGGATTTA+TGG - chr4.1:31938118-31938137 None:intergenic 35.0%
! CACAAAGACTAAAGTGTTCA+AGG + chr4.1:31939009-31939028 MS.gene45463:CDS 35.0%
! CTTGAACACTTTAGTCTTTG+TGG - chr4.1:31939011-31939030 None:intergenic 35.0%
! TCACTTGTTAGTGATGAGAA+TGG + chr4.1:31939034-31939053 MS.gene45463:CDS 35.0%
! TTTTGTAGAGGGATAGTTCT+AGG - chr4.1:31938887-31938906 None:intergenic 35.0%
!! CTTTAGAACCTAGAGCTTTA+TGG - chr4.1:31937725-31937744 None:intergenic 35.0%
!! GCCATTTTGATACATCTGAA+TGG + chr4.1:31939083-31939102 MS.gene45463:CDS 35.0%
!!! AAAACGGTGTAGTTTTGTAG+AGG - chr4.1:31938899-31938918 None:intergenic 35.0%
!!! AAACGGTGTAGTTTTGTAGA+GGG - chr4.1:31938898-31938917 None:intergenic 35.0%
AAACTTCTTGGACCGGAAAT+TGG - chr4.1:31938604-31938623 None:intergenic 40.0%
AAATGGCATATGGATATGGG+AGG - chr4.1:31938970-31938989 None:intergenic 40.0%
AATGGCATATGGATATGGGA+GGG - chr4.1:31938969-31938988 None:intergenic 40.0%
ACATCATTGTAGATTGGCAG+AGG + chr4.1:31937909-31937928 MS.gene45463:intron 40.0%
ACCCATAACCTTGAATCCAA+TGG + chr4.1:31938272-31938291 MS.gene45463:CDS 40.0%
AGAGGTAAGGAGAAACTTCT+TGG - chr4.1:31938616-31938635 None:intergenic 40.0%
AGTTTGGATGATCTTCACGT+TGG + chr4.1:31938563-31938582 MS.gene45463:CDS 40.0%
ATCCACATAAGATGGTTCAC+AGG - chr4.1:31939181-31939200 None:intergenic 40.0%
ATTTGGAGGGGGAAATGATA+TGG + chr4.1:31937314-31937333 MS.gene45463:intron 40.0%
CAACGGAGATCAAAATCAAG+TGG + chr4.1:31938124-31938143 MS.gene45463:CDS 40.0%
CATACAGAAGCTTTCAAGAC+AGG + chr4.1:31938533-31938552 MS.gene45463:CDS 40.0%
CCAATATCCCATAAAGCTCT+AGG + chr4.1:31937714-31937733 MS.gene45463:intron 40.0%
CCTTGTGAATTTAGACCTGT+CGG - chr4.1:31937630-31937649 None:intergenic 40.0%
CTCCGTTGGATTTATGGTTA+TGG - chr4.1:31938112-31938131 None:intergenic 40.0%
GACCATTGGATTCAAGGTTA+TGG - chr4.1:31938277-31938296 None:intergenic 40.0%
GGAGATAAAATGGAAGCTCT+TGG + chr4.1:31939055-31939074 MS.gene45463:CDS 40.0%
GGTTCAAATATCACAAAGGG+AGG + chr4.1:31937241-31937260 MS.gene45463:intron 40.0%
TAAGGAGAAACTTCTTGGAC+CGG - chr4.1:31938611-31938630 None:intergenic 40.0%
TCCAAATATCCATCAACTGG+AGG - chr4.1:31937301-31937320 None:intergenic 40.0%
TCCACATAAGATGGTTCACA+GGG - chr4.1:31939180-31939199 None:intergenic 40.0%
TGAAGTTATGTAAGCTCCGT+TGG - chr4.1:31938393-31938412 None:intergenic 40.0%
TGTAAGCTCCGTTGGATTTA+TGG - chr4.1:31938385-31938404 None:intergenic 40.0%
TGTAAGGACCATTGGATTCA+AGG - chr4.1:31938283-31938302 None:intergenic 40.0%
TGTAATGACCCTCCAATTTC+CGG + chr4.1:31938589-31938608 MS.gene45463:CDS 40.0%
TGTGTCAACAAATTCGAGCA+TGG - chr4.1:31938813-31938832 None:intergenic 40.0%
TTGTGACATCGAGAAAGGAA+TGG - chr4.1:31938657-31938676 None:intergenic 40.0%
! AAGGTTATGGGTCTTATACC+CGG - chr4.1:31938264-31938283 None:intergenic 40.0%
! CAACTCTTTTCAATCCCTGA+AGG + chr4.1:31938692-31938711 MS.gene45463:CDS 40.0%
! CCTAGAGCTTTATGGGATAT+TGG - chr4.1:31937717-31937736 None:intergenic 40.0%
!! AGTTGATGGATATTTGGAGG+GGG + chr4.1:31937303-31937322 MS.gene45463:intron 40.0%
!! CACTTGATTTTGATCTCCGT+TGG - chr4.1:31938126-31938145 None:intergenic 40.0%
!! CAGTTGATGGATATTTGGAG+GGG + chr4.1:31937302-31937321 MS.gene45463:intron 40.0%
!! CCAGTTGATGGATATTTGGA+GGG + chr4.1:31937301-31937320 MS.gene45463:intron 40.0%
!! TCCAGTTGATGGATATTTGG+AGG + chr4.1:31937300-31937319 MS.gene45463:intron 40.0%
!! TCCTCCAGTTGATGGATATT+TGG + chr4.1:31937297-31937316 MS.gene45463:intron 40.0%
!! TTAGCACCGTAGAGTTTTGT+AGG + chr4.1:31937437-31937456 MS.gene45463:intron 40.0%
!!! GCCACTTTTTTTGAGAGGTA+AGG - chr4.1:31938629-31938648 None:intergenic 40.0%
AAGTGCTCCTCTCATGGATT+TGG - chr4.1:31938729-31938748 None:intergenic 45.0%
ACAGGGAAGAAATGACACAC+TGG - chr4.1:31939163-31939182 None:intergenic 45.0%
AGACAACTTGGGATTAAGGC+TGG + chr4.1:31939127-31939146 MS.gene45463:CDS 45.0%
ATGGCATATGGATATGGGAG+GGG - chr4.1:31938968-31938987 None:intergenic 45.0%
ATGGTTATGGGTCCTATACC+CGG - chr4.1:31938099-31938118 None:intergenic 45.0%
CATCGAGAAAGGAATGGAGT+CGG - chr4.1:31938651-31938670 None:intergenic 45.0%
CCCTCCAAATATCCATCAAC+TGG - chr4.1:31937304-31937323 None:intergenic 45.0%
CCGACAGGTCTAAATTCACA+AGG + chr4.1:31937627-31937646 MS.gene45463:intron 45.0%
GACAACTTGGGATTAAGGCT+GGG + chr4.1:31939128-31939147 MS.gene45463:CDS 45.0%
GCTGCGAGATCAAAATCAAG+TGG + chr4.1:31937959-31937978 MS.gene45463:CDS 45.0%
GGATGACAAGCTACCCATTT+GGG - chr4.1:31938947-31938966 None:intergenic 45.0%
TCCCTGTGAACCATCTTATG+TGG + chr4.1:31939176-31939195 MS.gene45463:CDS 45.0%
TCTAACTCTCCTCCAGTTGA+TGG + chr4.1:31937289-31937308 MS.gene45463:intron 45.0%
TTCTTGGACCGGAAATTGGA+GGG - chr4.1:31938600-31938619 None:intergenic 45.0%
! GAGCATGGACTCTAGAGAAT+TGG - chr4.1:31938798-31938817 None:intergenic 45.0%
! GTGAATAAGTAGAGTGCCTG+AGG + chr4.1:31937525-31937544 MS.gene45463:intron 45.0%
!! AGAGGGATAGTTCTAGGTGA+GGG - chr4.1:31938881-31938900 None:intergenic 45.0%
!! TAGAGGGATAGTTCTAGGTG+AGG - chr4.1:31938882-31938901 None:intergenic 45.0%
!!! AGTCGGCCACTTTTTTTGAG+AGG - chr4.1:31938634-31938653 None:intergenic 45.0%
!! ATATATATATATATATATAG+GGG - chr4.1:31937562-31937581 None:intergenic 5.0%
!!! TATTTTAAATTTTAGTTATT+TGG + chr4.1:31937220-31937239 MS.gene45463:intron 5.0%
AGGGGTTGAAGTTTGACCTC+AGG - chr4.1:31937544-31937563 None:intergenic 50.0%
CCTCAAGCCAAATCCATGAG+AGG + chr4.1:31938719-31938738 MS.gene45463:CDS 50.0%
CTCTTCAAGTGCTCCTCTCA+TGG - chr4.1:31938735-31938754 None:intergenic 50.0%
CTTCTTGGACCGGAAATTGG+AGG - chr4.1:31938601-31938620 None:intergenic 50.0%
GGGAGTTGTGACATCGAGAA+AGG - chr4.1:31938662-31938681 None:intergenic 50.0%
GGGATGACAAGCTACCCATT+TGG - chr4.1:31938948-31938967 None:intergenic 50.0%
GGTCTTTGTCTCCCCTGTAA+CGG - chr4.1:31938771-31938790 None:intergenic 50.0%
TGGCTTGAGGAGATCCTTCA+GGG - chr4.1:31938709-31938728 None:intergenic 50.0%
TGGGAGATCTTCTACGGAGT+TGG - chr4.1:31938498-31938517 None:intergenic 50.0%
! AGCACTTGAAGAGTGCGAAG+GGG + chr4.1:31938742-31938761 MS.gene45463:CDS 50.0%
! CTTCTACGGAGTTGGTCGAT+GGG - chr4.1:31938490-31938509 None:intergenic 50.0%
! GAGCACTTGAAGAGTGCGAA+GGG + chr4.1:31938741-31938760 MS.gene45463:CDS 50.0%
! GGACTCTAGAGAATTGGCAC+AGG - chr4.1:31938792-31938811 None:intergenic 50.0%
! TCTTCTACGGAGTTGGTCGA+TGG - chr4.1:31938491-31938510 None:intergenic 50.0%
! TTCTACGGAGTTGGTCGATG+GGG - chr4.1:31938489-31938508 None:intergenic 50.0%
! TTGGCTTGAGGAGATCCTTC+AGG - chr4.1:31938710-31938729 None:intergenic 50.0%
!! CCTCTCATGGATTTGGCTTG+AGG - chr4.1:31938722-31938741 None:intergenic 50.0%
!! GAGGGATAGTTCTAGGTGAG+GGG - chr4.1:31938880-31938899 None:intergenic 50.0%
CGAAGGGGAAACCGTTACAG+GGG + chr4.1:31938757-31938776 MS.gene45463:CDS 55.0%
GCGAAGGGGAAACCGTTACA+GGG + chr4.1:31938756-31938775 MS.gene45463:CDS 55.0%
TGCGAAGGGGAAACCGTTAC+AGG + chr4.1:31938755-31938774 MS.gene45463:CDS 55.0%
! GGAGCACTTGAAGAGTGCGA+AGG + chr4.1:31938740-31938759 MS.gene45463:CDS 55.0%
! TCTACGGAGTTGGTCGATGG+GGG - chr4.1:31938488-31938507 None:intergenic 55.0%
!! AGTTCTAGGTGAGGGGTTGC+TGG - chr4.1:31938873-31938892 None:intergenic 55.0%
TCTAGGTGAGGGGTTGCTGG+TGG - chr4.1:31938870-31938889 None:intergenic 60.0%
! CTACGGAGTTGGTCGATGGG+GGG - chr4.1:31938487-31938506 None:intergenic 60.0%
! TTGGTCGATGGGGGGCCTTT+TGG - chr4.1:31938479-31938498 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31937108 31939210 31937108 ID=MS.gene45463
chr4.1 mRNA 31937108 31939210 31937108 ID=MS.gene45463.t1;Parent=MS.gene45463
chr4.1 exon 31937108 31937161 31937108 ID=MS.gene45463.t1.exon1;Parent=MS.gene45463.t1
chr4.1 CDS 31937108 31937161 31937108 ID=cds.MS.gene45463.t1;Parent=MS.gene45463.t1
chr4.1 exon 31937921 31939210 31937921 ID=MS.gene45463.t1.exon2;Parent=MS.gene45463.t1
chr4.1 CDS 31937921 31939210 31937921 ID=cds.MS.gene45463.t1;Parent=MS.gene45463.t1
Gene Sequence

>MS.gene45463

ATGGCTACTACACTTACACTTCAGTTTCTCACTTTAGTCCTTTTCTTCTTTATGGTAACTCTGAGTTATTATTTTAAATTTTAGTTACTAACTCAGTTATAGGGAGAGATATTATTTTAAATTTTAGTTATTTGGTTCAAATATCACAAAGGGAGGGAAACAAATTATCTCAGAAACAAATTCTAACTCTCCTCCAGTTGATGGATATTTGGAGGGGGAAATGATATGGCTTTTACATTTAGAATTGAATGCTCTTTTATTTAATAATATTTTTTGACAACTATTTAATATCATCTACAATTTTTTTTGAACTATTGTTTTATCAACACTTAGCACCGTAGAGTTTTGTAGGACATGATATTTTTTTTTTGGTTTAATAGTTGAATGACTGTAAATCCACTTTTAATTTTTTTTAAGGTGAATAAGTAGAGTGCCTGAGGTCAAACTTCAACCCCTATATATATATATATATATATATATATATATATATATATATATATATATTATGAAATATTCATACCGACAGGTCTAAATTCACAAGGACATACGGTTGTTTTAATTATTTTGTACTGAATTTAAATTTTCAATTAATTTTTCTTTGAGGTTCCAATATCCCATAAAGCTCTAGGTTCTAAAGAATTTTTCCCCTTGAAAGTCCTTGCAATTAAAACGTGATGTAATTAGTAATAGTGATATCAATTTGGCTAATCAATTGGGACACTAATAATTTATATTTTTTTTTTGAAATAGTCTAACAATTTATATTCTTCTCTCAAAAAAAAAAAATTTATATTCTCATTGACATCATTGTAGATTGGCAGAGGAATCAATGCGAGAGACTTGAAAGTAGAGCTGCGAGATCAAAATCAAGTGGATTTAAATGGTGCCTACATAACTGCTTATAATCATGAAGCCAAACAACCAGAATCAAATGAACCTTACATAACTGGATATGGTAAACATGAAGCCAAACAACCAAATTACATTACCGGGTATAGGACCCATAACCATAAATCCAACGGAGATCAAAATCAAGTGGATTCAAATGGTGCCTACATAACTGCTTATAATCATGAAGCCAAACAACCAGAATCAAATGAACCTTACATAACTGGATATGGTAAACATGAAGCCAAACAATCAAATTACATTACCGGGTATAAGACCCATAACCTTGAATCCAATGGTCCTTACATAACTCAATATCGTATAACTCAATATGGTAAACATAAAACCAAGCAATCAAATTACATTACCGGGTATAGGACCCATAACCATAAATCCAACGGAGCTTACATAACTTCATATGATAAACATGAAGGCAAGCAACCAAATTACATTACTGGGTACAAATATGATGTCATTGATCCAAAAGGCCCCCCATCGACCAACTCCGTAGAAGATCTCCCAATTTCTAACTTGGATCATACAGAAGCTTTCAAGACAGGATATTTCAGTTTGGATGATCTTCACGTTGGAAATGTAATGACCCTCCAATTTCCGGTCCAAGAAGTTTCTCCTTACCTCTCAAAAAAAGTGGCCGACTCCATTCCTTTCTCGATGTCACAACTCCCAAGTATTCTTCAACTCTTTTCAATCCCTGAAGGATCTCCTCAAGCCAAATCCATGAGAGGAGCACTTGAAGAGTGCGAAGGGGAAACCGTTACAGGGGAGACAAAGACCTGTGCCAATTCTCTAGAGTCCATGCTCGAATTTGTTGACACAATTATTGGTTCAAACGTCAAATATAGTATTCTAACCACCAGCAACCCCTCACCTAGAACTATCCCTCTACAAAACTACACCGTTTTAGAAGTATCACATGATATTGATGTTCCCAAATGGGTAGCTTGTCATCCCCTCCCATATCCATATGCCATTTACTATTGCCATTACATTGCCACAAAGACTAAAGTGTTCAAGGTCTCACTTGTTAGTGATGAGAATGGAGATAAAATGGAAGCTCTTGGAGTTTGCCATTTTGATACATCTGAATGGAACCCTAATCATATTATATTCAGACAACTTGGGATTAAGGCTGGGAAGAATTCTCCAGTGTGTCATTTCTTCCCTGTGAACCATCTTATGTGGATTCCAATGTAG
Protein sequence

>MS.gene45463.t1

MATTLTLQFLTLVLFFFMIGRGINARDLKVELRDQNQVDLNGAYITAYNHEAKQPESNEPYITGYGKHEAKQPNYITGYRTHNHKSNGDQNQVDSNGAYITAYNHEAKQPESNEPYITGYGKHEAKQSNYITGYKTHNLESNGPYITQYRITQYGKHKTKQSNYITGYRTHNHKSNGAYITSYDKHEGKQPNYITGYKYDVIDPKGPPSTNSVEDLPISNLDHTEAFKTGYFSLDDLHVGNVMTLQFPVQEVSPYLSKKVADSIPFSMSQLPSILQLFSIPEGSPQAKSMRGALEECEGETVTGETKTCANSLESMLEFVDTIIGSNVKYSILTTSNPSPRTIPLQNYTVLEVSHDIDVPKWVACHPLPYPYAIYYCHYIATKTKVFKVSLVSDENGDKMEALGVCHFDTSEWNPNHIIFRQLGIKAGKNSPVCHFFPVNHLMWIPM