AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056925


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056925.t1 MTR_1g058570 96.907 194 6 0 1 194 1 194 2.18e-136 379
MS.gene056925.t1 MTR_1g058540 79.082 196 39 1 1 194 1 196 1.02e-100 289
MS.gene056925.t1 MTR_1g058560 87.692 195 23 1 1 194 1 195 3.34e-100 288
MS.gene056925.t1 MTR_2g086740 56.818 176 76 0 18 193 44 219 1.98e-57 180
MS.gene056925.t1 MTR_7g011090 49.180 183 89 1 12 194 8 186 6.88e-55 172
MS.gene056925.t1 MTR_4g081880 43.784 185 99 2 9 193 7 186 7.07e-49 157
MS.gene056925.t1 MTR_5g041900 46.108 167 90 0 28 194 20 186 8.98e-43 142
MS.gene056925.t1 MTR_2g086740 57.944 107 45 0 18 124 44 150 1.84e-32 114
MS.gene056925.t1 MTR_0004s0210 35.849 159 89 4 31 187 3 150 2.16e-18 78.6
MS.gene056925.t1 MTR_7g108130 28.049 164 113 3 31 191 24 185 8.65e-14 67.0
MS.gene056925.t1 MTR_0097s0070 31.356 118 79 2 71 187 69 185 9.25e-13 64.3
MS.gene056925.t1 MTR_8g089300 31.013 158 96 4 40 187 30 184 1.10e-11 61.2
MS.gene056925.t1 MTR_3g064310 32.203 118 77 2 74 191 77 191 4.79e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056925.t1 AT2G36100 58.911 202 74 1 2 194 5 206 3.22e-72 217
MS.gene056925.t1 AT5G06200 61.929 197 70 2 2 193 5 201 9.82e-65 198
MS.gene056925.t1 AT3G11550 61.000 200 71 2 2 194 5 204 3.59e-63 194
MS.gene056925.t1 AT2G27370 58.929 168 69 0 26 193 52 219 5.76e-61 189
MS.gene056925.t1 AT5G15290 48.000 150 78 0 25 174 17 166 1.12e-47 154
MS.gene056925.t1 AT1G14160 40.606 165 98 0 30 194 45 209 1.35e-41 139
MS.gene056925.t1 AT1G03700 32.215 149 92 2 39 187 12 151 7.01e-19 79.7
MS.gene056925.t1 AT4G03540 33.058 121 72 2 55 175 28 139 2.78e-15 70.5
MS.gene056925.t1 AT1G17200 27.545 167 109 4 29 191 23 181 3.14e-14 68.6
MS.gene056925.t1 AT4G15630 28.492 179 108 5 25 191 19 189 7.25e-14 67.4
MS.gene056925.t1 AT5G44550 30.263 152 97 3 40 183 22 172 2.26e-12 63.2
MS.gene056925.t1 AT3G06390 28.571 133 90 2 36 163 37 169 4.21e-12 62.8

Find 44 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTAATGTTGCTTGATTC+TGG 0.093003 1.4:+48621641 None:intergenic
CTAGCCATTTGCAACCAATT+TGG 0.242258 1.4:-48620950 MS.gene056925:CDS
GTGATGTCTGCTTTGGCTCT+TGG 0.283856 1.4:-48620854 MS.gene056925:CDS
AAGGCCTGGAGGATGGAAAA+AGG 0.302153 1.4:-48621591 MS.gene056925:CDS
CCTTAGAATGAAATCCATTA+TGG 0.309686 1.4:+48621562 None:intergenic
CCATAATGGATTTCATTCTA+AGG 0.312115 1.4:-48621562 MS.gene056925:CDS
TTCCATCCTCCAGGCCTTGT+AGG 0.319831 1.4:+48621596 None:intergenic
TTACGATATCTAAGATAATA+AGG 0.319981 1.4:+48621231 None:intergenic
TGATGTCTGCTTTGGCTCTT+GGG 0.345325 1.4:-48620853 MS.gene056925:CDS
TATTGCCGCTGCCGCCTCAA+TGG 0.357738 1.4:-48621519 MS.gene056925:CDS
ATTGCCGCTGCCGCCTCAAT+GGG 0.364904 1.4:-48621518 MS.gene056925:CDS
TGGTAATGTTGCTTGATTCT+GGG 0.370302 1.4:+48621642 None:intergenic
ATGGATTTCATTCTAAGGTT+AGG 0.379588 1.4:-48621557 MS.gene056925:CDS
AGCTGCTTCCATAGTTTACT+TGG 0.387801 1.4:-48621002 MS.gene056925:CDS
GTTGGTTGTGATGTCTGCTT+TGG 0.390211 1.4:-48620861 MS.gene056925:CDS
GTGCACAGAAATCTCCAAAT+TGG 0.409853 1.4:+48620936 None:intergenic
ATGGAAATCAAGACACAAAC+TGG 0.414877 1.4:-48620973 MS.gene056925:CDS
AGCTGCAGCAGCACTTGAAG+TGG 0.421850 1.4:+48621021 None:intergenic
AAGCCTTGGTCCAGGCGCAT+GGG 0.441787 1.4:+48621256 None:intergenic
AAAGCCTTGGTCCAGGCGCA+TGG 0.443522 1.4:+48621255 None:intergenic
AGCGGCAATAGCCGCTGCTA+TGG 0.444367 1.4:+48621532 None:intergenic
ATCTCCAAATTGGTTGCAAA+TGG 0.445100 1.4:+48620946 None:intergenic
TGGTAGGGATAGAACTAAGT+AGG 0.473587 1.4:+48621304 None:intergenic
TGTTCCCATTGAGGCGGCAG+CGG 0.482406 1.4:+48621514 None:intergenic
CTAGTTGCACCTACAAGGCC+TGG 0.489392 1.4:-48621605 MS.gene056925:CDS
ATTGTGTGCCAAGTAAACTA+TGG 0.493367 1.4:+48620994 None:intergenic
GCTTGATTCTGGGACATCAA+TGG 0.503870 1.4:+48621652 None:intergenic
TGGCTACGACAGAGAATGGT+AGG 0.504458 1.4:+48621288 None:intergenic
CAATTCTAGTTGCACCTACA+AGG 0.507515 1.4:-48621610 MS.gene056925:CDS
CACCTACAAGGCCTGGAGGA+TGG 0.513895 1.4:-48621598 MS.gene056925:CDS
CTGATCACTTGTTCCCATTG+AGG 0.525536 1.4:+48621505 None:intergenic
AGCCTTGGTCCAGGCGCATG+GGG 0.531806 1.4:+48621257 None:intergenic
ATGATGGCTACGACAGAGAA+TGG 0.540767 1.4:+48621284 None:intergenic
ATAAGGAAAAGCCTTGGTCC+AGG 0.552077 1.4:+48621248 None:intergenic
AAGATAATAAGGAAAAGCCT+TGG 0.569952 1.4:+48621242 None:intergenic
ATCACTTGTTCCCATTGAGG+CGG 0.575884 1.4:+48621508 None:intergenic
AGTTGCATCATTTATTACTG+TGG 0.594258 1.4:-48620897 MS.gene056925:CDS
ATAGTTTACTTGGCACACAA+TGG 0.619739 1.4:-48620992 MS.gene056925:CDS
AAGGTTAGGTGCCATAGCAG+CGG 0.656083 1.4:-48621543 MS.gene056925:CDS
GGCTACGACAGAGAATGGTA+GGG 0.661247 1.4:+48621289 None:intergenic
GCCTTGGTCCAGGCGCATGG+GGG 0.663208 1.4:+48621258 None:intergenic
AGAATCAAGCAACATTACCA+AGG 0.678912 1.4:-48621639 MS.gene056925:CDS
GATTTCTGTGCACAGACAAG+TGG 0.683686 1.4:-48620926 MS.gene056925:CDS
GTTGCACCTACAAGGCCTGG+AGG 0.707990 1.4:-48621602 MS.gene056925:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTAATAAAAATATGATAA+GGG - chr1.4:48621345-48621364 MS.gene056925:intron 10.0%
!! TTGTTAATAAAAATATGATA+AGG - chr1.4:48621344-48621363 MS.gene056925:intron 10.0%
!! ATTAAATTGTATACATCAAT+CGG + chr1.4:48621384-48621403 None:intergenic 15.0%
!! GTTAATAAAAATATGATAAG+GGG - chr1.4:48621346-48621365 MS.gene056925:intron 15.0%
!!! GTATTAGCTTATTATAATTA+TGG + chr1.4:48621136-48621155 None:intergenic 15.0%
!!! AACTGTGTTTATGAAATTTT+AGG - chr1.4:48621447-48621466 MS.gene056925:CDS 20.0%
!!! TTACGATATCTAAGATAATA+AGG + chr1.4:48621278-48621297 None:intergenic 20.0%
!!! AACTAGAATTGTTTTTTCCT+TGG + chr1.4:48620887-48620906 None:intergenic 25.0%
!!! CAGGTTTTTTGTTATTTCAA+TGG - chr1.4:48621167-48621186 MS.gene056925:intron 25.0%
AAGATAATAAGGAAAAGCCT+TGG + chr1.4:48621267-48621286 None:intergenic 30.0%
AGTTGCATCATTTATTACTG+TGG - chr1.4:48621609-48621628 MS.gene056925:CDS 30.0%
CCTTAGAATGAAATCCATTA+TGG + chr1.4:48620947-48620966 None:intergenic 30.0%
GAAAGAACTGAGTGAAAAAA+GGG + chr1.4:48621032-48621051 None:intergenic 30.0%
TAAAAATATGATAAGGGGTG+CGG - chr1.4:48621351-48621370 MS.gene056925:intron 30.0%
TGAAAGAACTGAGTGAAAAA+AGG + chr1.4:48621033-48621052 None:intergenic 30.0%
!! ATGGATTTCATTCTAAGGTT+AGG - chr1.4:48620949-48620968 MS.gene056925:CDS 30.0%
!! ATTTTAGGTGTTTCTTACTC+TGG - chr1.4:48621462-48621481 MS.gene056925:CDS 30.0%
!! CCATAATGGATTTCATTCTA+AGG - chr1.4:48620944-48620963 MS.gene056925:CDS 30.0%
!!! AAGTTTTGAGTGTTACATAC+TGG + chr1.4:48621089-48621108 None:intergenic 30.0%
!!! TGTGGTTGTTTTAATCTTGT+TGG - chr1.4:48621627-48621646 MS.gene056925:CDS 30.0%
AGAATCAAGCAACATTACCA+AGG - chr1.4:48620867-48620886 MS.gene056925:CDS 35.0%
ATAGTTTACTTGGCACACAA+TGG - chr1.4:48621514-48621533 MS.gene056925:CDS 35.0%
ATCTCCAAATTGGTTGCAAA+TGG + chr1.4:48621563-48621582 None:intergenic 35.0%
ATGGAAATCAAGACACAAAC+TGG - chr1.4:48621533-48621552 MS.gene056925:CDS 35.0%
ATTGTGTGCCAAGTAAACTA+TGG + chr1.4:48621515-48621534 None:intergenic 35.0%
GCTAATACTTGAAACAATGC+AGG - chr1.4:48621148-48621167 MS.gene056925:intron 35.0%
TTTGAGTGTTACATACTGGA+AGG + chr1.4:48621085-48621104 None:intergenic 35.0%
! TGGTAATGTTGCTTGATTCT+GGG + chr1.4:48620867-48620886 None:intergenic 35.0%
!! TTGGTAATGTTGCTTGATTC+TGG + chr1.4:48620868-48620887 None:intergenic 35.0%
AGCTGCTTCCATAGTTTACT+TGG - chr1.4:48621504-48621523 MS.gene056925:CDS 40.0%
CTAGCCATTTGCAACCAATT+TGG - chr1.4:48621556-48621575 MS.gene056925:CDS 40.0%
GAAAAAGGGAACAGCCATAA+TGG - chr1.4:48620930-48620949 MS.gene056925:CDS 40.0%
GTGCACAGAAATCTCCAAAT+TGG + chr1.4:48621573-48621592 None:intergenic 40.0%
TGGTAGGGATAGAACTAAGT+AGG + chr1.4:48621205-48621224 None:intergenic 40.0%
! CAATTCTAGTTGCACCTACA+AGG - chr1.4:48620896-48620915 MS.gene056925:CDS 40.0%
ATAAGGAAAAGCCTTGGTCC+AGG + chr1.4:48621261-48621280 None:intergenic 45.0%
ATCACTTGTTCCCATTGAGG+CGG + chr1.4:48621001-48621020 None:intergenic 45.0%
ATGATGGCTACGACAGAGAA+TGG + chr1.4:48621225-48621244 None:intergenic 45.0%
CTGATCACTTGTTCCCATTG+AGG + chr1.4:48621004-48621023 None:intergenic 45.0%
GATTTCTGTGCACAGACAAG+TGG - chr1.4:48621580-48621599 MS.gene056925:CDS 45.0%
GTTGGTTGTGATGTCTGCTT+TGG - chr1.4:48621645-48621664 MS.gene056925:CDS 45.0%
!! GCTTGATTCTGGGACATCAA+TGG + chr1.4:48620857-48620876 None:intergenic 45.0%
!! TGATGTCTGCTTTGGCTCTT+GGG - chr1.4:48621653-48621672 MS.gene056925:CDS 45.0%
AAGGCCTGGAGGATGGAAAA+AGG - chr1.4:48620915-48620934 MS.gene056925:CDS 50.0%
AGGCCTGGAGGATGGAAAAA+GGG - chr1.4:48620916-48620935 MS.gene056925:CDS 50.0%
GGCTACGACAGAGAATGGTA+GGG + chr1.4:48621220-48621239 None:intergenic 50.0%
TGGCTACGACAGAGAATGGT+AGG + chr1.4:48621221-48621240 None:intergenic 50.0%
! AAGGTTAGGTGCCATAGCAG+CGG - chr1.4:48620963-48620982 MS.gene056925:CDS 50.0%
! GTTCCCTTTTTCCATCCTCC+AGG + chr1.4:48620922-48620941 None:intergenic 50.0%
!! GTGATGTCTGCTTTGGCTCT+TGG - chr1.4:48621652-48621671 MS.gene056925:CDS 50.0%
AGCTGCAGCAGCACTTGAAG+TGG + chr1.4:48621488-48621507 None:intergenic 55.0%
CTAGTTGCACCTACAAGGCC+TGG - chr1.4:48620901-48620920 MS.gene056925:CDS 55.0%
! TTCCATCCTCCAGGCCTTGT+AGG + chr1.4:48620913-48620932 None:intergenic 55.0%
AAAGCCTTGGTCCAGGCGCA+TGG + chr1.4:48621254-48621273 None:intergenic 60.0%
AAGCCTTGGTCCAGGCGCAT+GGG + chr1.4:48621253-48621272 None:intergenic 60.0%
AGCGGCAATAGCCGCTGCTA+TGG + chr1.4:48620977-48620996 None:intergenic 60.0%
ATTGCCGCTGCCGCCTCAAT+GGG - chr1.4:48620988-48621007 MS.gene056925:CDS 60.0%
CACCTACAAGGCCTGGAGGA+TGG - chr1.4:48620908-48620927 MS.gene056925:CDS 60.0%
GTTGCACCTACAAGGCCTGG+AGG - chr1.4:48620904-48620923 MS.gene056925:CDS 60.0%
TATTGCCGCTGCCGCCTCAA+TGG - chr1.4:48620987-48621006 MS.gene056925:CDS 60.0%
TGTTCCCATTGAGGCGGCAG+CGG + chr1.4:48620995-48621014 None:intergenic 60.0%
AGCCTTGGTCCAGGCGCATG+GGG + chr1.4:48621252-48621271 None:intergenic 65.0%
ATCATACGCCCCCATGCGCC+TGG - chr1.4:48621240-48621259 MS.gene056925:CDS 65.0%
! AGGCGCATGGGGGCGTATGA+TGG + chr1.4:48621241-48621260 None:intergenic 65.0%
GCCTTGGTCCAGGCGCATGG+GGG + chr1.4:48621251-48621270 None:intergenic 70.0%
GCCCCCATGCGCCTGGACCA+AGG - chr1.4:48621247-48621266 MS.gene056925:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 48620844 48621684 48620844 ID=MS.gene056925
chr1.4 mRNA 48620844 48621684 48620844 ID=MS.gene056925.t1;Parent=MS.gene056925
chr1.4 exon 48621440 48621684 48621440 ID=MS.gene056925.t1.exon1;Parent=MS.gene056925.t1
chr1.4 CDS 48621440 48621684 48621440 ID=cds.MS.gene056925.t1;Parent=MS.gene056925.t1
chr1.4 exon 48621235 48621358 48621235 ID=MS.gene056925.t1.exon2;Parent=MS.gene056925.t1
chr1.4 CDS 48621235 48621358 48621235 ID=cds.MS.gene056925.t1;Parent=MS.gene056925.t1
chr1.4 exon 48620844 48621059 48620844 ID=MS.gene056925.t1.exon3;Parent=MS.gene056925.t1
chr1.4 CDS 48620844 48621059 48620844 ID=cds.MS.gene056925.t1;Parent=MS.gene056925.t1
Gene Sequence

>MS.gene056925

ATGTCAACAACCATTGATGTCCCAGAATCAAGCAACATTACCAAGGAAAAAACAATTCTAGTTGCACCTACAAGGCCTGGAGGATGGAAAAAGGGAACAGCCATAATGGATTTCATTCTAAGGTTAGGTGCCATAGCAGCGGCTATTGCCGCTGCCGCCTCAATGGGAACAAGTGATCAGACTCTCCCTTTTTTCACTCAGTTCTTTCAATTTGAAGCTAGCTATGATAGCTTTACAACCTTCCAGTATGTAACACTCAAAACTTTTTTTTTTCTTTCAAAACAATAATCCATAATTATAATAAGCTAATACTTGAAACAATGCAGGTTTTTTGTTATTTCAATGGCAATAGTAGCTTCCTACTTAGTTCTATCCCTACCATTCTCTGTCGTAGCCATCATACGCCCCCATGCGCCTGGACCAAGGCTTTTCCTTATTATCTTAGATATCGTAAGCTTTTCTGTCTGTCTGTCTGTCTATCTATCTATCTATTCTATTTGTTGTTAATAAAAATATGATAAGGGGTGCGGCAAGTGACCGATTGATGTATACAATTTAATTAAGTTCAATGTTAAATATTATCATAAATTAATTCAACTTTGAAACTGTGTTTATGAAATTTTAGGTGTTTCTTACTCTGGCCACTTCAAGTGCTGCTGCAGCTGCTTCCATAGTTTACTTGGCACACAATGGAAATCAAGACACAAACTGGCTAGCCATTTGCAACCAATTTGGAGATTTCTGTGCACAGACAAGTGGTGCAGTAGTTGCATCATTTATTACTGTGGTTGTTTTAATCTTGTTGGTTGTGATGTCTGCTTTGGCTCTTGGGAAGCATTGA

Protein sequence

>MS.gene056925.t1

MSTTIDVPESSNITKEKTILVAPTRPGGWKKGTAIMDFILRLGAIAAAIAAAASMGTSDQTLPFFTQFFQFEASYDSFTTFQFFVISMAIVASYLVLSLPFSVVAIIRPHAPGPRLFLIILDIVFLTLATSSAAAAASIVYLAHNGNQDTNWLAICNQFGDFCAQTSGAVVASFITVVVLILLVVMSALALGKH